• Clb5 specificity depended on an interaction between a hydrophobic patch in Clb5 and a short sequence in the substrate (the RXL or Cy motif). (nature.com)
  • Phosphorylation of Clb5-specific targets during S phase was reduced by replacing Clb5 with Clb2 or by mutating the substrate RXL motif, confirming the importance of Clb5 specificity in vivo . (nature.com)
  • Figure 2: Clb5 specificity depends on an interaction between the Clb5 hydrophobic patch and an RXL motif in the substrate. (nature.com)
  • A- and B-type cyclins differentially modulate substrate specificity of cyclin-cdk complexes. (nature.com)
  • Brown, N. R., Noble, M. E., Endicott, J. A. & Johnson, L. N. The structural basis for specificity of substrate and recruitment peptides for cyclin-dependent kinases. (nature.com)
  • Therefore, comparison of the activity, mechanism, and substrate specificity of Trm10 homologs from yeast and other organisms will help to address whether the multiple homologs function redundantly, or whether there are additional, previously undescribed, cellular roles for the multiple Trm10 enzymes in higher eukaryotic organisms. (osu.edu)
  • Because of the low specificity of the CDK consensus, however, databases of protein sequences are expected to contain large numbers of matches by chance. (biomedcentral.com)
  • The yeast proteins appear to exhibit broad specificity transporting a wide range of di- and trivalent metal cations. (tcdb.org)
  • Initially distinguished on the basis of differences in electrophoretic mobility in starch gel, GANC and GANAB have been shown to have other differences, including those of substrate specificity. (unl.edu)
  • We propose that ribosome specificity may exist in mammals, providing evidence that one ribosomal protein can influence composition of the ribosome by regulating its own paralog. (prolekarniky.cz)
  • The majority of ERG genes downstream of zymosterol (ERG6 ERG2 ERG3 ERG5 and ERG4) showed little or no sequence homology with human genes (KEGG pathways (17)) HMN-214 but instead proteins with unrelated sequence performed comparable enzymatic activities. (bioinf.org)
  • The network generated for ERG25 ERG26 ERG27 ERG28 proteins (Fig. 3A S4) revealed as expected many interactions reflecting their participation in the linear ergosterol biosynthesis pathway (green circles in Fig. 3A) as well as additional interactions with genes annotated for functions in lipid synthesis and metabolism. (bioinf.org)
  • Integrated epigenetics of human breast cancer : synoptic investigation of targeted genes, microRNAs and proteins upon demethylation treatment. (unibas.ch)
  • We also show that human CDK targets are enriched for proteins that contain clustered consensus matches and, by searching human cell cycle genes, we predict several putative CDK targets, including the human orthologs of Schizosaccharomyces pombe CDC5 (CDC5L) and S. cerevisiae Cdc20p (CDC20). (biomedcentral.com)
  • This domain occurred 417 times on human genes ( 967 proteins). (umbc.edu)
  • Here we show that in Saccharomyces cerevisiae a single DSB causes transcriptional inhibition of proximal genes independently of Tel1/ATM and Mec1/ATR. (elifesciences.org)
  • The proteins involved in homologous recombination have to work around other processes that go on inside the nucleus, such as the transcription of DNA in genes into RNA molecules. (elifesciences.org)
  • In most eukaryotes studied, ubiquitin is encoded by two classes of genes: (i) The first comprises genes coding for a single copy of ubiquitin fused to ribosomal proteins (r-proteins), most commonly eL40 and eS31. (123dok.net)
  • Several molecular mechanisms with antifungal agents have been reported for C. albicans where insertions, deletions, and point mutations in genes codifying target proteins are frequently related to the antifungal drug resistance. (intechopen.com)
  • Analysis of a set of stationary phase genes in Saccharomyces cerevisiae. (le.ac.uk)
  • This thesis describes the characterisation of a set of six genes of the baker's yeast Saccharomyces cerevisiae, that are induced when a molasses-grown culture reaches the end of rapid fermentative growth. (le.ac.uk)
  • The rationale for the isolation of such genes was that their promoters may be useful in a biotechnological application, where the regulated expression of heterologous proteins is required. (le.ac.uk)
  • Two had been identified as the previously described genes, HSP26 and HXKl encoding the 26kDa heat shock protein and the glucose-repressible hexokinase, respectively. (le.ac.uk)
  • A further two, previously undescribed genes, encode a second small, 12kDa, stress-induced protein (HSP12) and a thiamine biosynthetic enzyme (THI4). (le.ac.uk)
  • Most yeast ribosomal protein genes are duplicated and their characterization has led to hypotheses regarding the existence of specialized ribosomes with different subunit composition or specifically-tailored functions. (prolekarniky.cz)
  • In yeast, ribosomal protein genes are generally duplicated and evidence has emerged that paralogs might have specific roles. (prolekarniky.cz)
  • A growing number of human diseases have been linked to mutations in genes encoding factors involved in ribosome biogenesis and protein synthesis [5] , [6] . (prolekarniky.cz)
  • Genes that encode the proteins of complement components or their isotypes are distributed throughout different chromosomes, with 19 genes comprising 3 significant complement gene clusters in the human genome. (medscape.com)
  • Although we did not identify any highly Clb2-specific substrates, we found that Clb2-Cdk1 possessed higher intrinsic kinase activity than Clb5-Cdk1, enabling efficient phosphorylation of a broad range of mitotic Cdk1 targets. (nature.com)
  • Identification of a cyclin-cdk2 recognition motif present in substrates and p21-like cyclin-dependent kinase inhibitors. (nature.com)
  • Schulman, B. A., Lindstrom, D. L. & Harlow, E. Substrate recruitment to cyclin-dependent kinase 2 by a multipurpose docking site on cyclin A. (nature.com)
  • A Modified KESTREL Search Reveals a Basophilic Substrate Consensus for the Saccharomyces cerevisiae Npr1 Protein Kinase. (unibas.ch)
  • We show that cyclin-dependent kinase (CDK) consensus motifs are frequently clustered in CDK substrate proteins. (biomedcentral.com)
  • The genome of Saccharomyces cerevisiae has revealed a closely related PAK-type protein kinase called Cla4. (berkeley.edu)
  • The Nak1 subfamily is part of a larger superfamily that includes the catalytic domains of other protein STKs, protein tyrosine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase. (umbc.edu)
  • This subfamily is composed of Schizosaccharomyces pombe Nak1, Saccharomyces cerevisiae Kic1p (kinase that interacts with Cdc31p) and related proteins. (umbc.edu)
  • Pleckstrin, the protein where this domain was first detected, is the major substrate of protein kinase C in platelets. (embl.de)
  • Previous analyses suggest that lack of normal cilia causes the small-body phenotype through the activation of a signaling pathway which consists of the EGL-4 cGMP-dependent protein kinase and the GCY-12 receptor-type guanylyl cyclase. (stanford.edu)
  • Protein kinase A (PKA) is an important mediator of many signal transduction pathways that occur in eukaryotic cells, and it has been implicated as a regulator of stage differentiation in Trypanosoma cruzi. (elsevierpure.com)
  • The yeast metal resistance proteins, which are 850-900 amino acyl residues in length, also exhibit two or three putative TMSs. (tcdb.org)
  • Highly conserved acidic residues found in the short periplasmic loop are not essential for CorA function or Mg 2+ selectivity but may be required for proper protein folding and stability. (tcdb.org)
  • The CorA proteins of E. coli and S. typhimurium are each 316 amino acyl residues in length. (tcdb.org)
  • STKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine residues on protein substrates. (umbc.edu)
  • These biological processes require the coordinated signal cascades of UPS members, including ubiquitin ligases, ubiquitin-conjugating enzymes, deubiquitinases, and proteasomes, to ubiquitination and de-ubiquitination on substrates. (bvsalud.org)
  • In vitro reconstitution of SCF substrate ubiquitination with purified proteins. (deshaieslab.com)
  • The TIM23 and the TIM22 complexes in the inner mitochondrial membrane are essential transport systems for proper insertion of inner membrane and matrix proteins in mitochondria. (uni-goettingen.de)
  • Together, these results expand our knowledge about the molecular interactions between mitochondrial translocase components during active protein import, as well as increase our repertoire of the TIM22 complex substrates. (uni-goettingen.de)
  • As a test case, we reconstruct the topology of the reaction and regulatory network for the mitochondrial ISC biogenesis pathway in S. cerevisiae . (biomedcentral.com)
  • Many of the mammalian Dbl family proteins are tissue-specific and their number in Metazoa varies in proportion of cell signaling complexity. (wikipedia.org)
  • They are members of the rBAT family of mammalian proteins (TC #8.A.9). (tcdb.org)
  • Unlike yeast, most mammalian ribosomal proteins are thought to be encoded by a single gene copy, raising the possibility that heterogenous populations of ribosomes are unique to yeast. (prolekarniky.cz)
  • Ribosome synthesis is a highly controlled process, whereby three distinct RNA polymerases are synchronously coordinated to produce equimolar amounts of four rRNAs and 79 mammalian ribosomal proteins (RPs) [1] - [4] . (prolekarniky.cz)
  • For this we used the yeast sterol pathway proteins as seeds to mine data from large-scale yeast genetic arrays (18) affinity purification and mass spectroscopy resolution of protein complexes (19-21) and protein complementation screens (22) to gain further insight into their function (Physique S4 Table S1 and supplemental Cytoscape file). (bioinf.org)
  • Unexpectedly multiple genetic and protein-protein interactions were HMN-214 also detected between and proteins with Gene Ontology (GO) annotations indicating direct involvement HMN-214 in vesicular transport secretory pathway and cellular localization: of 178 ERG25-interacting proteins 53 had such GO annotations representing a highly significant enrichment (e.g. vesicle-mediated transport p=1.4*10?8) (Fig. 3B). (bioinf.org)
  • The ubiquitin proteasome system (UPS) is a major protein degradation pathway in cells. (bvsalud.org)
  • The more than likely absence of known mechanistic and kinetic data for each of the individual proteins in a novel pathway hinders the process of translating network topology into a mathematical model. (biomedcentral.com)
  • The classical pathway is triggered by interaction of the Fc portion of an antibody (immunoglobulin [Ig] M, IgG1, IgG2, IgG3) or C-reactive protein with C1q. (medscape.com)
  • for example, the proteins factor H and factor I inhibit the formation of the enzyme C3 convertase of the alternative pathway. (medscape.com)
  • Protein kinases are critical to cellular signalling and post-translational gene regulation, but their biological substrates are difficult to identify. (biomedcentral.com)
  • Protein kinases are ubiquitous components of cellular signalling networks [ 1 ]. (biomedcentral.com)
  • The ability of cells to respond to extracellular signals is mediated by signal transduction networks that almost invariably include a cascade of protein kinases. (berkeley.edu)
  • One family of protein kinases that is universally conserved in eukaryotes is called the p21-activated protein kinases (PAKs). (berkeley.edu)
  • Catalytic domain of Fungal Nak1-like Protein Serine/Threonine Kinases. (umbc.edu)
  • Ser/Thr protein kinases such as the Akt/Rac family, the beta-adrenergic receptor kinases, the mu isoform of PKC and the trypanosomal NrkA family. (embl.de)
  • Tyrosine protein kinases belonging to the Btk/Itk/Tec subfamily. (embl.de)
  • Our structure explains how this TPR sub-complex, together with additional scaffolding subunits (Apc1, Apc4 and Apc5), coordinate the juxtaposition of the catalytic and substrate recognition module (Apc2, Apc11 and Apc10 (also known as Doc1)), and TPR-phosphorylation sites, relative to co-activator, regulatory proteins and substrates. (ox.ac.uk)
  • Together, these suggest the dynamic nature of interactions within the subunits of the PAM complex, as well as between TOM and TIM23 subunits, during the process of translocation of a protein into mitochondria. (uni-goettingen.de)
  • About 24% of these proteins were phosphorylated more efficiently by Clb5-Cdk1 than Clb2-Cdk1. (nature.com)
  • C. elegans hypothetical proteins C04D8.1, K06H7.4 and ZK632.12. (embl.de)
  • S. cerevisiae hypothetical proteins YBR129c and YHR155w. (embl.de)
  • Eighteen of these were hypothetical proteins with unknown functions, while the others had putative or known functions. (elsevierpure.com)
  • Enzymatic analysis of recombinant SIRT2 in comparison to a yeast homolog of Sir2 protein (Hst2p) shows a striking preference of SIRT2 for acetylated tubulin peptide as a substrate relative to acetylated histone H3 peptide. (nih.gov)
  • Familia de enzimas reguladoras homólogas, estructuralmente relacionadas con la proteína 2 reguladora de información silente del tipo sexual (Sir2), que se encuentra en Saccharomyces cerevisiae. (bvsalud.org)
  • A homologous family of regulatory enzymes that are structurally related to the protein silent mating type information regulator 2 (Sir2) found in Saccharomyces cerevisiae. (bvsalud.org)
  • GTPases are active when bound to GTP and inactive when bound to GDP, allowing their activity to be regulated by GEFs and the opposing GTPase activating proteins (GAPs). (wikipedia.org)
  • The binding of GEFs to their GTPase substrates catalyzes the dissociation of GDP, allowing a GTP molecule to bind in its place. (wikipedia.org)
  • GAPs (GTPase-activating protein) act antagonistically to inactivate GTPases by increasing their intrinsic rate of GTP hydrolysis. (wikipedia.org)
  • Though this general scheme is common among GEFs, the specific interactions between the regions of the GTPase and GEF vary among individual proteins. (wikipedia.org)
  • Some GEFs are specific to a single GTPase while others have multiple GTPase substrates. (wikipedia.org)
  • Regulators of small G-proteins like guanine nucleotide releasing factor GNRP (Ras-GRF) (which contains 2 PH domains), guanine nucleotide exchange proteins like vav, dbl, SoS and Saccharomyces cerevisiae CDC24, GTPase activating proteins like rasGAP and BEM2/IPL2, and the human break point cluster protein bcr. (embl.de)
  • Charting the protein complexome in yeast by mass spectrometry. (deshaieslab.com)
  • Therefore, when facultatively fermentative yeasts are used to produce biomass, or products directly de- rived from biomass such as (heterologous) proteins, ac- curate control of the dissolved-oxygen concentration is a necessity when glucose is the carbon source. (9lib.org)
  • Yeasts, especially S. cerevisiae and other non-saccharomyces yeasts today are increasingly used for the heterologous production of enzymes and pharmaceutical proteins. (enhalor.com)
  • Having in mind diversity and potential of all yeast species, the cultivation and utilization of Saccharomyces yeasts are still the tip of the iceberg and there is a vast potential yet to be discovered for the production of valuable products using saccharomyces and non-saccharomyces yeasts. (enhalor.com)
  • We contextualized the PTM sites in structural models of the MTases and revealed that many fell in catalytic pockets or enzyme-substrate interfaces. (edu.au)
  • These same principles of mechanistic enzymology, in combination with the powerful approaches of yeast genetics and molecular biology, also allow the lab to investigate the biological function of enzymes in living cells, including a tRNA splicing enzyme and a tRNA methyltransferase whose biological roles in the yeast Saccharomyces cerevisiae are well-defined, but whose possible role(s) in other organisms remain a mystery. (osu.edu)
  • 2003). Trm10, like Thg1, is a member of a previously undescribed family of proteins with no identifiable homology to any other enzyme family, including other S-adenosyl-methionine (SAM)-dependent methyltransferases. (osu.edu)
  • All GH31 enzymes cleave a terminal carbohydrate moiety from a substrate that varies considerably in size, depending on the enzyme, and may be either a starch or a glycoprotein. (unl.edu)
  • α-Glucosidase (α-D-Glucosidase), a carbohydrate hydrolyzing enzyme, catalyzes the liberation of α-glucose from the non-reducing end of the substrate. (medchemexpress.com)
  • To understand the biology of PKA, identification of the particular substrates of this enzyme is essential. (elsevierpure.com)
  • Allergen avoidance strategies including personal protective equipment, engineering controls, protein encapsulation, and reduction of airborne enzyme concentrations are required to mitigate occupational exposure to fungal enzymes. (cdc.gov)
  • Among all sterol metabolizing enzymes and their corresponding substrates ERG1 ERG7 ERG11 ERG24 ERG25 ERG26 ERG27 were conserved between Saccharomyces cerevisiae and humans such that proteins with high levels of sequence homology performed comparable functions in sterol biosynthesis HMN-214 (Fig. 1 S4A S4B and Table S1). (bioinf.org)
  • Our data suggest that regulatory modules may exist in protein sequence as clusters of short sequence motifs. (biomedcentral.com)
  • A beta-hairpin comprising the nuclear localization sequence sustains the self-associated states of nucleosome assembly protein 1. (colorado.edu)
  • It is generated by proteolytic cleavage of precursor proteins in which it is fused either to itself, constituting a polyubiquitin precursor of head-to-tail monomers, or as a single N-terminal moiety to ribosomal proteins. (123dok.net)
  • Understanding the role of the ubiquitin fused to ribosomal proteins becomes relevant, as these proteins are practically invariably eS31 and eL40 in the different eukaryotes. (123dok.net)
  • Herein, we used the amenable yeast Saccharomyces cerevisiae to study whether ubiquitin facilitates the expression of the fused eL40 (Ubi1 and Ubi2 precursors) and eS31 (Ubi3 precursor) ribosomal proteins. (123dok.net)
  • We conclude that ubiquitin might serve as a cis-acting molecular chaperone that assists in the folding and synthesis of the fused eL40 and eS31 ribosomal proteins. (123dok.net)
  • Ubiquitin fusion to other r-proteins, such as P1 or P2, and even to non-ribosomal proteins such as actin, has been reported in diverse genera of rare single-celled algae as, for example, Bigelowiella [6]. (123dok.net)
  • In eukaryotes, secretory proteins are first translocated into the ER by the Sec61 complex before reaching the cell surface by vesicular trafficking. (biorxiv.org)
  • XylS is a glycosyl hydrolase family 31 (GH31) alpha-xylosidase found in prokaryotes, eukaryotes, and archaea, that catalyzes the release of alpha-xylose from the non-reducing terminal side of the alpha-xyloside substrate. (unl.edu)
  • To achieve such a goal, strategies that combine the different theoretical and computational methods to identify proteins and generate a set of plausible alternative network topologies for the process of interest are needed. (biomedcentral.com)
  • In the yeast Saccharomyces cerevisiae , which is widely applied for industrial bioethanol production, uptake of hexoses is mediated by transporters with a facilitated diffusion mechanism. (biomedcentral.com)
  • their products enable the cell to utilise alternative hexoses as growth substrates. (le.ac.uk)
  • The TIM22 complex is required for the import of polytopic inner membrane proteins which lack a presequence but have internal targeting signals. (uni-goettingen.de)
  • The Sec61 complex forms a protein-conducting channel in the endoplasmic reticulum (ER) membrane that is required for secretion of soluble proteins and production of many membrane proteins. (biorxiv.org)
  • The Sec61 complex also mediates membrane integration of many proteins, including most cell surface receptors and cell adhesion molecules. (biorxiv.org)
  • In addition, the channel has a seam (lateral gate) in the wall that can open laterally in the plane of the membrane to release transmembrane segments (TMs) of membrane protein clients into the lipid phase. (biorxiv.org)
  • Ubiquitin functions as a reversible post-translational modifier of proteins to regulate many different cellular processes such as DNA repair, chromatin dynamics, cell cycle regulation, membrane and protein trafficking, endocytosis, autophagy, but most notably proteasome-dependent protein degradation [2-4]. (123dok.net)
  • Rhomboids are an ancient and conserved family of intramembrane-cleaving proteases, a small group of proteolytic enzymes capable of hydrolyzing a peptide bond within a transmembrane helix that anchors a substrate protein to the membrane. (ulaval.ca)
  • The important components of this system are various cell membrane-associated proteins such as complement receptor 1 (CR1), complement receptor 2 (CR2), and decay accelerating factor (DAF). (medscape.com)
  • A large variety of proteins containing the WW domain are known. (embl.de)
  • Pleckstrin homology (PH) domains are small modular domains that occur in a large variety of proteins. (embl.de)
  • The universally conserved heterotrimeric Sec61 complex (SecY in prokaryotes) plays essential roles in biosynthesis of more than one third of proteins in all species (for review, see ref. 1 - 4 ). (biorxiv.org)
  • Many facultatively fermentative yeast species exhibit a 'Kluyver effect': even under oxygen-limited growth con- ditions, certain disaccharides that support aerobic, respi- ratory growth are not fermented, even though the compo- nent monosaccharides are good fermentation substrates. (9lib.org)
  • Saccharomyces cerevisiae has been described as mankind's most domesticated organism and still widely exploited yeast species in industry today. (enhalor.com)
  • Inhibition of protein synthesis by TOR inactivation revealed a conserved regulatory mechanism of the BiP chaperone in Chlamydomonas. (unibas.ch)
  • However, the lack of structural information of the TIM23 complex prevents us from completely deciphering the exact mechanism for the import of a presequence-containing substrate. (uni-goettingen.de)
  • In such environments, the high extracellular glucose concentration can drive the uptake of glucose by yeast cells via diffusion, which is exemplified by the occurrence of many hexose transporters with a facilitated diffusion mechanism in S. cerevisiae . (biomedcentral.com)
  • S. cerevisiae is largest cultivated organism so far. (enhalor.com)
  • While the human (h) ETF has been studied in great detail, very little is known about the biochemical properties of the homologous protein in the model organism Saccharomyces cerevisiae (yETF). (elsevierpure.com)
  • Here, we report that a human ortholog of Sir2p, sirtuin type 2 (SIRT2), is a predominantly cytoplasmic protein that colocalizes with microtubules. (nih.gov)
  • Several other sirtuin members utilize NAD to transfer ADP-RIBOSE to proteins and are categorized as MONO ADP-RIBOSE TRANSFERASES, while a third group of sirtuins appears to have both deacetylase and ADP ribose transferase activities. (bvsalud.org)
  • The smaller proteins are generally of prokaryotic origin while the larger ones are of eukaryotic origin. (tcdb.org)
  • The larger eukaryotic and archaeal proteins possess N- and C-terminal hydrophilic extensions. (tcdb.org)
  • Ubiquitin is a highly conserved small eukaryotic protein. (123dok.net)
  • Ubiquitin is a small eukaryotic protein of 76 amino acids whose name (i.e., it occurs ubiquitously) results from its remarkable evolutionary conservation [1]. (123dok.net)
  • Domain commonly found in eukaryotic signalling proteins. (embl.de)
  • For example, the Ran GEF, RCC1, is present in the nucleus while the Ran GAP is present in the cytosol, modulating nuclear import and export of proteins. (wikipedia.org)
  • This process is carried out by the human spliceosome machinery, in which over 300 proteins sequentially assemble with uridine-rich small nuclear RNA molecules (U snRNAs) to form distinct small nuclear ribonucleoprotein complexes (snRNPs). (biomedcentral.com)
  • Organellar proteomics reveals hundreds of novel nuclear proteins in the malaria parasite Plasmodium falciparum. (unibas.ch)
  • Cytoskeletal proteins such as dynamin (see IPR001401 ), Caenorhabditis elegans kinesin-like protein unc-104 (see IPR001752 ), spectrin beta-chain, syntrophin (2 PH domains) and S. cerevisiae nuclear migration protein NUM1. (embl.de)
  • Mouse protein citron, a putative rho/rac effector that binds to the GTP-bound forms of rho and rac. (embl.de)
  • Protein methylation is one of the major post-translational modifications (PTMs) in the cell. (edu.au)
  • Figure 3: Clb5-specific substrate phosphorylation occurs in vivo . (nature.com)
  • In this study, we designed and generated new proteins for formation of the TOM-TIM23 supercomplex in organello and in vivo. (uni-goettingen.de)
  • The silent information regulator 2 protein (Sir2p) of Saccharomyces cerevisiae is an NAD-dependent histone deacetylase that plays a critical role in transcriptional silencing. (nih.gov)
  • Structural biology: Corralling a protein-degradation regulator. (deshaieslab.com)
  • Information on how its 13 constituent proteins are assembled, and how they interact with co-activators, substrates and regulatory proteins is limited. (ox.ac.uk)
  • Deficiency of any of these regulatory proteins results in a state of overactivation of the complement system, with damaging inflammatory effects. (medscape.com)
  • Microorganisms capable of catabolizing lignin-derived ar- carbohydrate and protein components of the organic matter omatics are essentially fungi and bacteria (Bugg et al. (lu.se)
  • We show that it is possible to predict proteins that are likely to be targets of CDKs in S. cerevisiae by searching for proteins that contain clustered matches to the CDK consensus. (biomedcentral.com)
  • Finally, we examine co-clustering of the CDK consensus motifs with the 'cy' or RXL motif [ 17 ], which is known to be important in determining which CDK-cyclin complex will phosphorylate a given substrate. (biomedcentral.com)
  • However, there are some similarities in how different GEFs alter the conformation of the G protein nucleotide-binding site. (wikipedia.org)
  • UV-triggered affinity capture identifies interactions between the Plasmodium falciparum multidrug resistance protein 1 (PfMDR1) and antimalarial agents in live parasitized cells. (unibas.ch)
  • WW domain-mediated interactions reveal a spliceosome-associated protein that binds a third class of proline-rich motif: the proline glycine and methionine-rich motif. (embl.de)
  • In yeast, this bridging involves interactions between the WW domains in the splicing factor PRP40 and a proline-rich domain in the branchpoint binding protein, BBP. (embl.de)
  • Through these interactions, PH domains play a role in recruiting proteins to different membranes, thus targeting them to appropriate cellular compartments or enabling them to interact with other components of the signal transduction pathways. (embl.de)
  • Thus, CNNM proteins, the vertebrate orthologues of CorB/C, also have Mg 2+ transport capacity. (tcdb.org)
  • Overexpression of the yeast proteins, Al R 1p and Mn R 2p, overcomes toxicity to aluminum and manganese, respectively. (tcdb.org)
  • Parts derived from human and yeast proteins are outlined with solid and dashed lines, respectively. (biorxiv.org)
  • This precompiled information serves as a substrate and matrix to embed your contributions, but it is by no means the final word - Homo sapiens can do much better! (wikigenes.org)
  • These glycoproteins include the CD98 heavy chain protein of Mus musculus (gbU25708) and the orthologous 4F2 cell surface antigen heavy chain of Homo sapiens (spP08195). (tcdb.org)
  • α-Synuclein is an abundant brain protein that binds to lipid membranes and is involved in the recycling of presynaptic vesicles. (ox.ac.uk)
  • Microalgae can efficiently absorb CO 2 in the atmosphere and turn it into abundant high-value products, including polysaccharides, lipids, proteins, pigments, and biofuels [2-5]. (researchsquare.com)
  • Small angle neutron scattering (SANS) provides unique insight into biomacromolecular complexes by combining solvent contrast variation (H2O:D2O exchange) with either natural contrast between different classes of biomolecules (proteins, RNA/DNA, lipids/detergents) and/or by applying artificial contrast, i.e. deuteration of specific biomolecules. (lu.se)
  • Therefore, the conversion of the DSB ends from double-stranded to single-stranded DNA, which is necessary to initiate DSB repair by homologous recombination, is responsible for loss of transcription around a DSB in S. cerevisiae . (elifesciences.org)
  • Additionally, both complexes recognise different targeting signals on a precursor protein. (uni-goettingen.de)
  • In this study, the TIM23 and the TIM22 complexes were investigated regarding their structure and substrate spectrum respectively. (uni-goettingen.de)
  • The TOM and the TIM23 complexes cooperate for importing presequence-containing proteins into mitochondria. (uni-goettingen.de)
  • In a first couple of examples, I will show how distance and shape restraints from SANS have helped to improve the uniqueness of structural models for two multi-protein-RNA complexes, in combination with NMR restraints and building blocks from crystallography [1, 2]. (lu.se)
  • The large ~280-kDa U5 snRNP protein PRPF8 is central to the dynamics of spliceosome assembly [ 11 ]. (biomedcentral.com)
  • Moreover, FBP21 interacts directly with the U1 snRNP protein U1C, the core snRNP proteins SmB and SmB', and the branchpoint binding protein SF1/mBBP. (embl.de)
  • Some animal proteins, for example, those in the LAT family (TC# 2.A.3.8) including ASUR4 (gbY12716) and SPRM1 (gbL25068) associate with a type 1 transmembrane glycoprotein that is essential for insertion or activity of the permease and forms a disulfide bridge with it. (tcdb.org)
  • Hydropathy analysis had predicted two transmembrane α-helical spanners (TMSs) in the C-terminal regions of these proteins. (tcdb.org)
  • These proteins have predominantly been defined to contain either four or six transmembrane domains. (uni-goettingen.de)
  • 2006 ) who identified a soluble oligomeric N-terminal domain of the E. coli CorA that appeared to be tetrameric and to bind its substrates with the same affinity of native CorA ( Wang et al . (tcdb.org)
  • Two-step affinity purification of multiubiquitylated proteins from Saccharomyces cerevisiae. (deshaieslab.com)
  • Therefore, many of the matches in protein sequences are likely to be false-positive predictions. (biomedcentral.com)
  • Predictions regarding how proteins act in ISC biogenesis are validated by comparison with published experimental results. (biomedcentral.com)
  • Several S. cerevisiae proteins involved in cell cycle regulation and bud formation like BEM2, BEM3, BUD4 and the BEM1-binding proteins BOI2 (BEB1) and BOI1 (BOB1). (embl.de)