NucleotideSpecificityDouble-strandedQuadruplexMutationViralProteinSequencesGeneConformationComplementaryDetectionAminoStructuresCassetteTransportChangeLargeSelectionAnalysisDomainStepFormsConformation polymorphismSNPsGeneticInducesFunctionalCharacterizationMotifsMolecularAnalysisImpactIncludeCellDetectionShownPatients
Nucleotide2
- So far, more than 4580 single nucleotide polymorphisms (SNPs) for this gene have been identified. (wikipedia.org)
- Nanoscaffolding and nanoquenching properties of thiol-capped gold nanocrystals (GNCs), covalently linked to fluorophore-lebeled oligonucleotide through metal-sulfur bond, were extensively studied for decades to detect specific sequences and single-nucleotide mismatches [ 1 - 3 , 21 , 22 ]. (hindawi.com)
Specificity1
- We address the molecular basis of their broad substrate specificity gleaned from structural information and discuss novel approaches to block the function of ABC transporters. (nature.com)
Double-stranded2
- During the life cycle of HBV, RT utilizes a single-stranded viral genomic RNA as a template to synthesize a hybrid RNA-DNA duplex, and then converts it to double-stranded DNA (ds-DNA). (biomedcentral.com)
- The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. (lookformedical.com)
Quadruplex4
- Dramatic effect of furanose C2' substitution on structure and stability: directing the folding of the human telomeric quadruplex with a single fluorine atom. (ub.es)
- Indeed, given that four guanine residues can self-associate in a planar arrangement through a Hoogsteen-type hydrogen-bonding network [ 4 ] to form a structure called G-quartet, G-rich strands can adopt a peculiar three-dimensional arrangement called G-quadruplex DNA [ 5 - 9 ] resulting from the stacking of several contiguous G-quartets (Figure 1 ). (hindawi.com)
- The formation of G-quadruplex DNA is easily conceivable in DNA sequences that are present as single strands in cells, such as the telomeric overhang. (hindawi.com)
- Nevertheless, we have to keep in mind that these G-quadruplex-forming sequences although transiently single-stranded are a priori not as easily accessible as the telomeric sequences, due to the presence of the transcription machinery [ 28 ]. (hindawi.com)
Mutation1
- The ORF1b:V2354F mutation, corresponding to NSP15:V303F, may induce a conformational change and result in a disruption to a flanking beta-sheet structure. (biomedcentral.com)
Viral1
- Because of potential issues with controlling this earlier manufacturing process, which included scraping VACV lesions from calfskin, new vaccines are being developed and manufactured by using viral propagation on well-characterized cell substrates. (cdc.gov)
Protein3
- More precisely, the protein is predicted to be composed of 62.9% α-helix, 33.8% random coil, and 3.3% extended strand. (wikipedia.org)
- PARP inhibitors can trap the PARP-1 protein at a single-stranded break/DNA lesion and disrupt its catalytic cycle, ultimately leading to replication fork progression and consequent double-strand breaks. (biomedcentral.com)
- Hop (Hsp-organizing protein) promotes the transfer of the substrate from the Hsp70 machinery to Hsp90. (encyclopedia.pub)
Sequences1
- These regions are interspaced with repeat sequences that are identical or almost identical within a single CRISPR cassette. (pjmonline.org)
Gene3
- The SLC46A3 gene, also known by its aliases solute carrier family 46 member 3 and FKSG16, is located at 13q12.3 on the reverse strand in humans. (wikipedia.org)
- In this paper, we have targeted a 27-bp AluI fragment of porcine mitochondrial cytochrome b (cytb) gene to construct porcine-specific hybrid nanobiosensor probe that was able to detect pork in heterogeneous autoclaved meat products in a single step eliminating the need of washing or separation steps. (hindawi.com)
- Our results show that one out of 60 bulls tested exhibited polymorphism (G→T) at position 559 in exon 4 of SLC35A3 gene. (researchgate.net)
Conformation1
- Hsp70 undergoes large allosteric changes upon ATP hydrolysis and substrate binding, from an extended, ADP-bound conformation with the substrate-binding domain (SBD) closed by the lid (PDB code 2kho) [ 4 ] to a collapsed, ATP-bound conformation with the SBD opened, favoring substrate release (PDB code 5nro) [ 5 ] . (encyclopedia.pub)
Complementary1
- G-rich DNA strands are naturally involved in duplex-DNA architecture through association with their complementary C-rich DNA strands by the canonical Watson-Crick pairing [ 1 - 3 ]. (hindawi.com)
Detection1
- These results demonstrate clearly the potential for multiplexed sequence detection in large DNA strands and open the possibility for profihng multiple polymorphic sites on DNA ema forex in the 10 kilobase or larger size range. (findbinaryoption.com)
Amino1
- Expanding the substrate scope of pyrrolysyl-transfer RNA synthetase enzymes to include non-σ-amino acids in vitro and in vivo. (harvard.edu)
Structures2
- We utilized a hybrid computational approach to model the atomistic structures of HBV-RT/DNA-RNA/dATP and HBV-RT/DNA-RNA/TFV-DP (tenofovir diphosphate) complexes with the native hybrid DNA-RNA substrate in place. (biomedcentral.com)
- these structures differ by strand orientation (grey dashed arrows) and loop arrangement (represented in orange, yellow, and red). (hindawi.com)
Cassette1
- Fig. 3: Substrate-binding pockets of ATP-binding cassette transporters and the drug transport cycle. (nature.com)
Transport3
- It is mainly involved in the transport of small molecules across the membrane through the substrate translocation pores featured in the MFS domain. (wikipedia.org)
- MFS and MFS_1 domains largely overlap and contain 42 putative substrate translocation pores that are predicted to bind substrates for transmembrane transport. (wikipedia.org)
- Srikant, S. & Gaudet, R. Mechanics and pharmacology of substrate selection and transport by eukaryotic ABC exporters. (nature.com)
Change1
- The substrate translocation pores have access to both sides of the membrane in an alternating fashion through a conformational change. (wikipedia.org)
Large1
- The HCV genome contains approximately 10,000 nucleotides, which are translated into a single large polyprotein. (xiahepublishing.com)
Selection1
- We describe, from a regulatory perspective, the various strains of VACV, the adverse events associated with calf lymph-propagated smallpox vaccine, the issues regarding selection and use of cell substrates for vaccine production, and the issues involved in demonstrating evidence of safety and efficacy. (cdc.gov)
Analysis1
- The aim of this study was to detect carriers of CVM in Holstein population using Polymerase Chain Reaction- Primer-introduced restriction analysis and Restriction Fragment Length Polymorphism (PCR-PIRA and RFLP) methods. (researchgate.net)
Domain1
- Hsp40 regulates the substrate folding and ATP hydrolysis by Hsp70 (Hsp40 J domain is included in PDB 5nro in the blue ribbon representation). (encyclopedia.pub)
Step1
- The biosensor detected 0.5% and 1% pork in raw and 2.5-h autoclaved pork-beef binary admixtures in a single step without any separation or washing. (hindawi.com)
Forms1
- Active forms of NRTIs incorporate into the elongating DNA strand, terminate the elongation of the nascent DNA strand, and prevent additional dNTPs from being incorporated. (biomedcentral.com)
Conformation polymorphism1
- Deletions and point mutations in the hGR gene of cells selected for steroid resistance in vitro were investigated by PCR-single strand conformation polymorphism analysis. (aacrjournals.org)
SNPs4
- Polymerase chain reaction (PCR) single-strand conformational polymorphism analysis was used to identify single-nucleotide polymorphisms (SNPs) in all of the exons and at the exon-intron boundaries, and PCR-restriction fragment length polymorphism analysis and DNA sequencing were used to determine the frequencies of the newly identified variant alleles in the four major ethnic groups. (nih.gov)
- So far, more than 4580 single nucleotide polymorphisms (SNPs) for this gene have been identified. (wikipedia.org)
- Genomic markers including restriction fragment length polymorphisms (RFLPs), short tandem repeats (STRs, also called microsatellites), insertion-deletion polymorphisms, single nucleotide polymorphisms (SNPs) and groups of markers inherited together on one chromosome as haplotypes are being used to locate disease-associated genetic loci, and studies of the association between these loci and disease are elucidating the genetic basis for disease. (cdc.gov)
- Whole blood provides high quality genomic DNA in microgram quantities sufficient for current applications including genome scans using SNPs or STRs, polymorphism discovery using methods such as denaturing gel electrophoresis, single strand conformational polymorphisms (SSCP), or sequencing, and for genotyping loci using methods such as allele specific oligonucleotides, RFLPs, or sequencing. (cdc.gov)
Genetic4
- The aim of this study was to identify any genetic polymorphisms of the CYP2A13 gene, which may alter the metabolic capacities of the enzyme. (nih.gov)
- The Japan Esophageal Cohort study showed that esophageal SCC and head and neck SCC that developed after endoscopic resection for esophageal SCC were associated with genetic polymorphisms related to alcohol metabolism. (bvsalud.org)
- We investigated the association between the development of second primary esophageal squamous-cell carcinoma after endoscopic resection for esophageal squamous-cell carcinoma and genetic polymorphisms related to alcohol and nicotine metabolism. (bvsalud.org)
- The frequencies of alcohol dehydrogenase 1B, aldehyde dehydrogenase 2 and cytochrome P450 2A6 genetic polymorphisms in the buccal mucosa were analyzed. (bvsalud.org)
Induces2
- This study demonstrates that trifluridine induces single-strand DNA damage in ESCC cells, as evidenced by phosphorylated replication protein 32. (bvsalud.org)
- This induces a conformational change, leading to autoactivation of C1r that then cleaves C1s to its active state. (medscape.com)
Functional1
- The new shop alafrangalığın tarihi geleneğin tasfiyesi ya da yeniden üretilmesi is presented to transfer much subscription-based noncovalent memory exclusive dynamics for functional nAChRs as such Hartree-Fock( TD-HF), Configuration Interactions Singles( CIS), and Several system classical book( TD-DFT). (atelier-im-revierpark.de)
Characterization1
- Finally, we will explore how single cell RNA sequencing could bring the characterization of renal progenitors to the next level, while knowing their molecular signature is gaining relevance in the clinic. (preprints.org)
Motifs3
- To the best of our knowledge, all currently described amyloid structures consist of a repetitive stacking of intricate, serpentine, planar β-strand arrangements that are stabilized by steric zipper motifs wherein interdigitated residue side chains make extensive Van der Waals, electrostatic, hydrophobic and hydrogen bonding contacts. (nature.com)
- This gene encodes one of the PPIases, which specifically binds to phosphorylated ser/thr-pro motifs to catalytically regulate the post-phosphorylation conformation of its substrates. (cancerindex.org)
- These are three-strand motifs that contain characteristic TRYPTOPHAN, ARGININE, and CYSTEINE residues respectively. (nih.gov)
Molecular1
- Bruno Canard a obtenu un doctorat en 1991 en microbiologie et biochimie de l'Université Paris VII à l'Institut Pasteur (Dir : Dr. Stewart T. Cole), Paris, et une formation post-doctorale en 1995-1998 à la Harvard Medical School, Dept. Biological Chemistry and Molecular Pharmacology (Dir : Dr. Charles C. Richardson), Boston. (univ-mrs.fr)
Analysis1
- Single-strand conformational polymorphism and heteroduplex analysis of the coding region of the regulatory p85α subunit in cDNA isolated from human muscle tissue from 70 insulin-resistant NIDDM patients and 12 control subjects revealed three silent polymorphisms and a missense mutation at nucleotide position 1020 (G→A), changing a Met to He at codon 326. (diabetesjournals.org)
Impact1
- The conformational regulation catalyzed by this PPIase has a profound impact on key proteins involved in the regulation of cell growth, genotoxic and other stress responses, the immune response, induction and maintenance of pluripotency, germ cell development, neuronal differentiation, and survival. (cancerindex.org)
Include3
- Factors governing the choice of fluorophore molecule to be used for detection include the sample type, substrate, light emission spectra characteristics and the number of target proteins to be studied. (medscape.com)
- Beta-Coronaviruses are enveloped positive strand RNA viruses that include major human and animal pathogens such as Middle East Respiratory Syndrome Virus (MERS) and the Severe Acute Respiratory Syndrome Viruses SARS CoV1 and SARS CoV2. (nih.gov)
- Expanding the substrate scope of pyrrolysyl-transfer RNA synthetase enzymes to include non-σ-amino acids in vitro and in vivo. (harvard.edu)
Cell3
- Because of potential issues with controlling this earlier manufacturing process, which included scraping VACV lesions from calfskin, new vaccines are being developed and manufactured by using viral propagation on well-characterized cell substrates. (cdc.gov)
- We describe, from a regulatory perspective, the various strains of VACV, the adverse events associated with calf lymph-propagated smallpox vaccine, the issues regarding selection and use of cell substrates for vaccine production, and the issues involved in demonstrating evidence of safety and efficacy. (cdc.gov)
- The development of anatomical structures to create the form of a single- or multi-cell organism. (lookformedical.com)
Detection2
- [ 5 ] Fluorescence detection is not compatible for detection of all substrates due to the inherent autofluorescence property of some materials, which decreases the signal-to-noise ratio. (medscape.com)
- [ 44 ] Direct labeling requires only a single capture antibody, has the capacity for multiplexed detection of hundreds of analytes, and offers accurate and reproducible results for abundant proteins. (medscape.com)
Shown1
- Trifluridine combined with a WEE1 inhibitor increased trifluridine-mediated DNA damage, namely DNA double-strand breaks, as shown by increased γ-H2AX expression. (bvsalud.org)
Patients1
- However, resistance to steroid therapy is a frequent phenomenon, and early studies showed that patients who relapse after single-agent induction of an initial remission are generally refractory to further steroid therapy (1, 2, 3, 4) . (aacrjournals.org)