• amino acid
  • Plant and bacterial cystathionine beta-lyases are inhibited by the antimicrobial amino acid, L-aminoethoxybinylglycine (AVG), and the antibacterial amino acid, rhizobitoxine. (wikipedia.org)
  • The enzyme is required for ketogenesis in the liver, and is also responsible for processing the amino acid leucine inside the mitochondrion Mutations in the HMGCL gene cause 3-hydroxy-3-methylglutaryl-CoA lyase deficiency (HMGCLD), a rare autosomal recessive inborn error of metabolism characterized by disruption of ketogenesis and L-leucine catabolism. (wikipedia.org)
  • reaction
  • In biochemistry, a lyase is an enzyme that catalyzes the breaking (an "elimination" reaction) of various chemical bonds by means other than hydrolysis (a "substitution" reaction) and oxidation, often forming a new double bond or a new ring structure. (wikipedia.org)
  • A combination of both an elimination and a Michael addition is seen in O-succinylhomoserine (thiol)-lyase (MetY or MetZ) which catalyses first the γ-elimination of O-succinylhomoserine (with succinate as a leaving group) and then the addition of sulfide to the vinyl intermediate, this reaction was first classified as a lyase (EC 4.2.99.9), but was then reclassified as a transferase (EC 2.5.1.48). (wikipedia.org)
  • Adenylosuccinate lyase deficiency, also called adenylosuccinase deficiency, is a rare autosomal recessive metabolic disorder characterized by the appearance of succinylaminoimidazolecarboxamide riboside (SAICA riboside) and succinyladenosine (S-Ado) in cerebrospinal fluid, urine.These two succinylpurines are the dephosphorylated derivatives of SAICA ribotide (SAICAR) and adenylosuccinate (S-AMP), the two substrates of adenylosuccinate lyase (ADSL), which catalyzes an important reaction in the de novo pathway of purine biosynthesis. (wikipedia.org)
  • His68 and His141 seem to serve as the general acid and base catalysts, and are critical to the catalyzing reaction of the substrate. (wikipedia.org)
  • mechanism
  • The precise molecular mechanism by which selenols, generated by β-lyase, provide protection against cisplatin-induced cytotoxicity, however, remains to be established. (aspetjournals.org)
  • As shown in the mechanism below, cystathionine beta-lyase facilitates the S-C bond cleavage in cystathionine with the use of a PLP cofactor bounded to a catalytic lysine residue. (wikipedia.org)
  • Class I AP endonucleases (EC 4.2.99.18) cleave 3' to AP sites by a β-lyase mechanism, leaving an unsaturated aldehyde, termed a 3'-(4-hydroxy-5-phospho-2-pentenal) residue, and a 5'-phosphate. (wikipedia.org)
  • structurally
  • The cystathionine beta-lyase monomer consists of three functionally and structurally distinct domains: Composed of three α-helices and one beta-strand that contribute to the formation of the quaternary structure. (wikipedia.org)
  • methionine
  • Three-dimensional structures of noncovalent complexes of Citrobacter freundii methionine γ-lyase with substrates. (sigmaaldrich.com)
  • Crystal structures of Citrobacter freundii methionine γ-lyase complexes with the substrates of γ- (L-1-amino-3-methylthiopropylphosphinic acid) and β- (S-ethyl-L-cysteine) elimination reactions and the competitive inhibitor L-norleucine have been determined at 1.45, 1.8, and 1.63 Å resolution, respectively. (sigmaaldrich.com)
  • Found in plants, bacteria, and yeast, cystathionine beta-lyase is an essential part of the methionine biosynthesis pathway as homocysteine can be directly converted into methionine by methionine synthase. (wikipedia.org)
  • Other members include cystathionine gamma-synthase, cystathionine gamma-lyase, and methionine gamma lyase. (wikipedia.org)
  • Treatment of adenylosuccinate lyase deficiency can be done via epilepsy management with anticonvulsive drugs.Additionally the following options include: D-ribose and uridine administration Ketogenic diet S-adenosyl-l-methionine The prognosis of this condition in childhood usually has a stable outcome, whereas in neonatal is almost always fatal, according to Jurecka, et al. (wikipedia.org)
  • Hevea
  • Commonly studied (S)-selective hydroxynitrile lyases include MeHNL from Manihot esculenta and HbHNL from Hevea brasiliensis. (wikipedia.org)
  • structures
  • The binary complex of the truncated enzyme with cyanocobalamin and the ternary complex with cyanocobalamin or adeninylpentylcobalamin and substrates were crystallized, and their x-ray structures were analyzed. (pubmedcentralcanada.ca)
  • cleavage
  • This subclass contains the decarboxylases ( EC 4.1.1 ), the aldehyde-lyases catalysing the reversal of an aldol condensation ( EC 4.1.2 ), and the oxo-acid-lyases, catalysing the cleavage of a 3-hydroxy acid ( EC 4.1.3 ), or the reverse reactions. (qmul.ac.uk)
  • inhibitor
  • Arg374 and Ser339 are involved in the binding of carboxyl groups of the substrates and the inhibitor. (sigmaaldrich.com)
  • The protective activity against cisplatin-induced cytotoxicity and ROS generation was blocked by aminooxyacetic acid, a selective inhibitor of pyridoxal 5′-phosphate-dependent cysteine conjugate β-lyases, further supporting the role of β-lyase in the observed chemoprotection. (aspetjournals.org)
  • Molecule
  • When acting on the single substrate, a molecule is eliminated and this generates either a new double bond or a new ring. (qmul.ac.uk)
  • deficiency
  • In addition, the lysine 48 residue which is mutated in patients with 3-hydroxy-3-methylglutaryl-CoA lyase deficiency is also found to be necessary for substrate binding. (wikipedia.org)
  • Among the signs and symptoms of adenylosuccinate lyase deficiency are the following: Aggressive behavior Microcephaly Autism Brachycephaly Mild Cerebellar hypoplasia Seizures Adenylosuccinate lyase deficiency is responsible for a range of symptoms that involve psychomotor retardation, often accompanied by epileptic seizures, and autistic features. (wikipedia.org)
  • molecular
  • To rationalize the observed structure-affinity relationships of aliginate lyase alyVI with its (GGG) substrate, molecular docking, MD imulations and binding free energy calculations followed by site-directed mutagenesis and alyVI activity assays were carried out. (sigmaaldrich.com)
  • gene
  • Cloning and Sequence Analysis of a Gene(aly PG) Encoding Poly (α-L-guluronate) lyase from Corynebacterium sp. (nii.ac.jp)
  • 3-hydroxy-3-methylglutaryl-CoA lyase (or HMG-CoA lyase) is an enzyme that in human is encoded by the HMGCL gene located on chromosome 1. (wikipedia.org)