• Moreover, we found that glioblastoma cells, notorious for their VEGF production, elicited a similar response when co-cultured with endothelial cells [11] . (plos.org)
  • Conditioned media from such cells were then used to stimulate human aortic endothelial cells (HAoECs) to enhance tube-like structure formation in a Matrigel assay. (hindawi.com)
  • Neither AT alone in control media nor conditioned media nor AT-stimulated cells affected numbers of endothelial cells in the plug or plug's vascularization. (hindawi.com)
  • In contrast to monolayer cultures, however, the spheroid structures that are formed when ESCs aggregate enables the non-adherent culture of EBs in suspension, making EB cultures inherently scalable, which is useful for bioprocessing approaches, whereby large yields of cells can be produced for potential clinical applications. (wikipedia.org)
  • When cultured as single cells in the absence of anti-differentiation factors, ESCs spontaneously aggregate to form EBs. (wikipedia.org)
  • Such spontaneous formation is often accomplished in bulk suspension cultures whereby the dish is coated with non-adhesive materials, such as agar or hydrophilic polymers, to promote the preferential adhesion between single cells, rather than to the culture substrate. (wikipedia.org)
  • As hESC undergo apoptosis when cultured as single cells, EB formation often necessitates the use of inhibitors of the rho associated kinase (ROCK) pathway, including the small molecules Y-27632 and 2,4 disubstituted thiazole (Thiazovivin/Tzv). (wikipedia.org)
  • Alternatively, to avoid dissociation into single cells, EBs can be formed from hESCs by manual separation of adherent colonies (or regions of colonies) and subsequently cultured in suspension. (wikipedia.org)
  • While this method enables control of EB size by altering the number of cells per drop, the formation of hanging drops is labor-intensive and not easily amenable to scalable cultures. (wikipedia.org)
  • Under AAS culture conditions, the cell killing effect of GEF was synergistically pronounced in the EGFR-expressing cell lines, namely, CAL 27, Detroit 562, A549 and PANC-1 cells compared with those treated with either GEF or AAS alone. (spandidos-publications.com)
  • Electron microscopy demonstrated that the CAL 27 cells treated with GEF under AAS culture conditions exhibited swelling of the cytosol and organelles with an increased number of autophagosomes and autolysosomes, but without chromatin condensation and nuclear fragmentation. (spandidos-publications.com)
  • Our study is aimed at investigating whether precursors of myeloid angiogenic cells (PACs) treated with known pharmaceuticals would produce media with better proangiogenic activity in vitro and if such media can be used to stimulate blood vessel growth in vivo . (hindawi.com)
  • CD34 + cells after six days in culture were stimulated with atorvastatin (AT), acetylsalicylic acid (ASA), sulforaphane (SR), resveratrol (RV), or metformin (Met) for 48 h. (hindawi.com)
  • Then, we used atorvastatin-stimulated cells or conditioned media from them in the Matrigel plug in vivo angiogenic assay. (hindawi.com)
  • In our study, we worked on cells derived from the CD34 + hematopoietic stem and progenitor cells, enriched with CD133 + population, and used culture media promoting growth of hematopoietic progenitors. (hindawi.com)
  • Use of serum to culture cells dates back nearly 70 years and continues to this day, albeit with less devotion. (genengnews.com)
  • In one stage culture strategy, cells were treated by sodium tungstate at the beginning of culture. (hindawi.com)
  • More recently, increased expression of NOTCH pathway genes was implicated in some DIPGs and NOTCH path inhibition contributed to reduced tumor growth in culture 14 , but the specificity of these treatments for H3.3 mutant cells remains unclear. (nature.com)
  • After 6-8 days of culture, non-adherent cells were washed off with room temperature PBS and macrophages were obtained as a homogeneous population of adherent cells which were scraped and subsequently seeded onto tissue culture-treated petri dishes overnight in RPMI 1640 containing 10% FCS, 100 U/ml penicillin/streptomycin, and 10ng/ml M-CSF. (nih.gov)
  • there is a need to quantify the forces that are collectively exerted by invading cancer cells under physiologically relevant conditions. (elifesciences.org)
  • Finally, the action of GLP-1 on β-cell mass expansion is abolished in both transgenic mice and cultured β-cells with increased dosage of SirT1. (diabetesjournals.org)
  • Moreover, GLP-1 has been shown to promote β-cell mass expansion in both experimental animal models ( 8 , 9 ) and cultured β-cells ( 7 , 10 - 14 ). (diabetesjournals.org)
  • Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. (wikipedia.org)
  • After the cells of interest have been isolated from living tissue , they can subsequently be maintained under carefully controlled conditions. (wikipedia.org)
  • Most cells require a surface or an artificial substrate to form an adherent culture as a monolayer (one single-cell thick), whereas others can be grown free floating in a medium as a suspension culture . (wikipedia.org)
  • Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. (wikipedia.org)
  • The lifespan of most cells is genetically determined, but some cell-culturing cells have been "transformed" into immortal cells which will reproduce indefinitely if the optimal conditions are provided. (wikipedia.org)
  • In practice, the term "cell culture" now refers to the culturing of cells derived from multicellular eukaryotes , especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture , fungal culture, and microbiological culture (of microbes ). (wikipedia.org)
  • Viral culture is also related, with cells as hosts for the viruses. (wikipedia.org)
  • In 1907 the zoologist Ross Granville Harrison demonstrated the growth of frog embryonic cells that would give rise to nerve cells in a medium of clotted lymph . (wikipedia.org)
  • [12] He suggested that the potentialities of individual cells via tissue culture as well as that the reciprocal influences of tissues on one another could be determined by this method. (wikipedia.org)
  • Cells were incubated overnight or for 7 days with fresh 1:1 media mixture added to existing media every 2 days before being fixed in 4%.Not all cells in a given raw image were traced. (biotech-angels.com)
  • Furthermore, the degree of the proliferation and osteogenic differentiation of mesenchymal stem cells (MSCs) co-cultured with HGC-27 or SGC-7901 cells was analyzed by colony-formation assay, alizarin red staining, immunofluorescence, qPCR, immunoblotting, and alkaline phosphatase activity assay. (aging-us.com)
  • Cultured cells also produce rich ECM, particularly stromal cells. (bvsalud.org)
  • Gaining better insight into ECM derived from either tissue or cells that regulate 3D tissue reconstruction or organ regeneration helps us to select, produce, and implant the most suitable ECM and thus promote 3D organoid culture and tissue remodeling for in vivo regeneration. (bvsalud.org)
  • Recently, the development of with food (in particular dietary fibre, comprising more than 100 trillion mi- molecular tools and subsequently of enabling energy harvest from oth- crobes living in the intestines, the next-generation sequencing enabled erwise indigestible dietary com- mouth, the skin, the vagina, and the richness of the intestinal ecosys- pounds), interacting with cells (in- elsewhere in the body. (who.int)
  • Each individ- cluding immune cells, but also the was previously cal ed the gastroin- ual harbours hundreds of different metabolic and nervous systems), testinal flora or microflora, the more species, most of which have not and protecting against pathogens pragmatic term "microbiota" is now yet been cultured. (who.int)
  • BMDM Isolation: Bone marrow was harvested and washed once with PBS, seeded/cultured in DMEM containing 20 % FCS, 30 % L-cell conditioned media (as source of macrophage colony stimulating factor, M-CSF), and 100 U/ml penicillin/streptomycin in non-tissue culture-treated petri dishes. (nih.gov)
  • Medium transfer from OGD-treated astrocytes to untreated neurons induced protection against OGD in neurons, which was attenuated strongly by the application of sEpoR and anti-EpoR. (jneurosci.org)
  • Additionally, 100 mM HS directly inhibited NKCC1 up-regulation in astrocytes under hypoxic condition. (biomedcentral.com)
  • In vitro , the sprouting and migratory properties of primary endothelial cell cultures were reduced by inhibiting EZH2 through up-regulation of miR-101, siRNA-mediated knockdown of EZH2, or treatment with 3-Deazaneplanocin-A (DZNep), a small molecule inhibitor of EZH2 methyltransferase activity. (plos.org)
  • Cellular ECM improves 3D culture development in vitro and tissue remodeling during wound healing after implantation into the host as well. (bvsalud.org)
  • Overall, the decellularization methodologies and tissue/cell-derived ECM as scaffolds or cellular-growth supplements used in cell propagation and differentiation for 3D tissue culture in vitro are discussed. (bvsalud.org)
  • And all the yeast which he cultured from the lesions of skin at the sample sites was clinically nor- pityriasis versicolor [ 1 ]. (who.int)
  • however, two-stage culture raised lipids to 50% in which neutral lipids were enhanced 2.14 times. (hindawi.com)
  • Previous studies have demonstrated that the quantity of lipids varies according to the change of growth conditions such as nutrients concentrations, light intensity, or temperature [ 6 , 7 ]. (hindawi.com)
  • This enables microalgae to accumulate a significant amount of neutral lipids under stress conditions. (hindawi.com)
  • Similar to ESCs cultured in monolayer formats, ESCs within embryoid bodies undergo differentiation and cell specification along the three germ lineages - endoderm, ectoderm, and mesoderm - which comprise all somatic cell types. (wikipedia.org)
  • The term "tissue culture" was coined by American pathologist Montrose Thomas Burrows . (wikipedia.org)
  • The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture . (wikipedia.org)
  • [6] In 1885 Wilhelm Roux removed a section of the medullary plate of an embryonic chicken and maintained it in a warm saline solution for several days, establishing the basic principle of tissue culture. (wikipedia.org)
  • Gottlieb Haberlandt first pointed out the possibilities of the culture of isolated tissues, plant tissue culture . (wikipedia.org)
  • This is necessary to compare growth of plants from tissue culture with that of plants from normal vegetative propagation methods. (aff.org.au)
  • 2. Virkon and tissue culture hood with UV-light. (lu.se)
  • 3. 15-cm tissue culture-treated plastic dishes. (lu.se)
  • PAHs could be degraded by bacteria under aerobic conditions through the initial oxidation of the aromatic ring, which is catalyzed by the dioxygenase enzyme. (omicsonline.org)
  • Methods: To achieve this, oral bacteria were isolated using a culture-omics method from 30 samples collected from healthy individuals. (bvsalud.org)
  • These bacteria were then cultured in three different types of media under both aerobic and anaerobic conditions. (bvsalud.org)
  • Subsequently, we performed the isolation of lytic and lysogenic phages targeting all these bacteria. (bvsalud.org)
  • The NRA yields drug-susceptibility information to isoniazid and rifampin 21-28 days after inoculating a smear-positive sputum sample (direct method) ( 10 ) or 8-10 days after obtaining a positive culture (indirect method). (cdc.gov)
  • The pooled sensitivity and specificity of the NRA (on culture isolates and sputum) have been reported to be 97% and 100% for rifampin and 96% and 99% for isoniazid ( 11 ). (cdc.gov)
  • Mycobacterial species isolated from sputum and grown in culture were examined to distinguish between NTM and the Mycobacterium tuberculosis complex (MTBC). (who.int)
  • NTM were detected in 4% (9/225) of sputum samples grown in culture. (who.int)
  • 4. Refractory MAC lung infection, defined as having positive MAC cultures while being treated with a multidrug mycobacterial antibiotic treatment regimen for a minimum of 6 consecutive months and no documented successful treatment, defined as negative sputum culture for MAC and cessation of treatment. (who.int)
  • Others use dextran, but it adheres to culture vessel walls and subsequently removes cholesterol from the media creating a sink. (genengnews.com)
  • Hydroperoxidase-deficient hktE - pgdx - H. influenzae Rd showed a slightly affected aerobic growth phenotype in rich broth, while, in chemically defined medium, growth was completely inhibited by aerobic conditions, unless the medium contained an amino acid/vitamin supplement. (biomedcentral.com)
  • [3] This is typically facilitated via use of a liquid, semi-solid, or solid growth medium , such as broth or agar . (wikipedia.org)
  • This signaling circuit is crucial in renal defenses against uropathogenic Escherichia coli [UPEC, ( 32 )] under regular diet conditions as well as in cutaneous macrophage-driven antimicrobial responses against the protozoan parasite Leishmania major under experimental HS diet ( 8 ). (frontiersin.org)
  • Analysis of genome-wide microarrays reveals 2.8% of transcripts whose expression was regulated in at least one of the four experimental conditions: 47.3% of which were repressed by CRE1, whereas 29.0% were actually induced by CRE1, and 17.2% only affected by the growth rate but CRE1 independent. (biomedcentral.com)
  • In each experimental condition, serial dilutions (10-1 to 10-3) were obtained and 25 μL aliquots were seeded in Sabouraud Dextrose Agar duplicate. (bvsalud.org)
  • Purgatory", 1975), constitutes rare examples on how public authority and commercial film production merged with the more venturesome aspects of experimental film culture. (lu.se)
  • The study focuses on two coherent areas of experimental film culture, which are conceptually placed pre- contra post the inauguration of the Swedish Film Institute. (lu.se)
  • By adapting a media-archaeological and discourse analytical approach these films and their experimental features are used in a summary manner to probe the complex relations between minor and major film cultures within Swedish historiography. (lu.se)
  • Since epidermal growth factor receptor (EGFR)-tyrosine kinase inhibitors, including gefitinib (GEF) have been reported to induce the apoptosis of several cancer cell lines, in the present study, we examined whether the cytotoxic effects of GEF are further enhanced under amino acid starvation (AAS) culture conditions. (spandidos-publications.com)
  • We have previously shown that GLP-1 transactivates the epidermal growth factor receptor ( 12 ) to subsequently activate phosphatidylinositol-3 kinase and Akt signaling ( 7 , 11 ). (diabetesjournals.org)
  • The percent area below the threshold was then calculated by setting the region of interest to the epidermal layer of the organotypic culture and calculating the area appearing positive for melanin. (biotech-angels.com)
  • We found that we could boost protein biomanufacturing of mAbs by adding the supplement even to a previously optimized serum-free media in a scalable bioreactor run representative of 10,000 L bioreactor runs. (genengnews.com)
  • H. influenzae colonizes the nasopharynx of up to 75% of the population, from where the Hib strains in particular can invade the bloodstream and subsequently pass to the central nervous system. (biomedcentral.com)
  • Quantification of dendrite length in 2D culture MCs were grown to 75% confluence in MC media and subsequently switched to a 1:1 ratio of KC-conditioned media:MC media. (biotech-angels.com)
  • SPS was added to the culture medium at various concentrations in time-of-addition assay. (molvis.org)
  • SB203580 and SP600125, specific inhibitors of the p38 and JNK signaling pathways, were used to treat primary microglia cultures to determine whether the two signaling pathways were required for the inhibition of HS on microglia expressing and secreting TNF-α and IL-1β using Western blotting, double immunofluorescence and enzyme-linked immunosorbent assay (ELISA). (biomedcentral.com)
  • H3.3 mutant gliomas are also particularly sensitive to NOTCH pathway gene knockdown and drug inhibition, reducing their viability in culture. (nature.com)
  • The samples were subsequently subjected to full-length 16S rRNA gene sequencing for analysis. (bvsalud.org)
  • Here we chose to use chemostat cultures on D-glucose as a carbon source at two different growth rates (one repressing and one derepressing [ 16 ]) to investigate the genome-wide changes in gene expression in relation to CRE1 function, using a Δcre1 recombinant mutant strain of T. reesei and corresponding control strain. (biomedcentral.com)
  • The optimal conditions for biobleaching were a mixture of xylanase and laccase crude extracts (1 to 2 v/v), at a temperature of 36 °C and a pH of 5.5. (ncsu.edu)
  • In contrast to the influx of amino acids, the degradation of proteins is also the important intracellular mechanism for releasing free amino acids both under steady-state conditions and during cellular stresses. (spandidos-publications.com)
  • In contrast, medium transfer from OGD-treated neurons to untreated neurons induced protection against OGD that did not involve EPO. (jneurosci.org)
  • Every expression system-every scheme for optimizing nutrients and cell culture conditions-presents advantages, disadvantages, and tradeoffs with respect to costs, yields, quality metrics, contaminants, availability of system components, time constraints, and so on. (genengnews.com)
  • Whereas inhibition of p38/MAPK did not result in improved neutrophil defense, pharmacological blockade of the phagocyte oxidase (PHOX) or its genetic ablation mimicked the impaired antimicrobial activity detected under high salt conditions. (frontiersin.org)
  • The method is based on a nitrate-reductase colorimetric reaction that uses Lowenstein-Jensen (LJ) medium prepared with antimicrobial drugs ( 9 ). (cdc.gov)
  • Additionally, the media can not be easily exchanged within the traditional hanging drop format, necessitating the transfer of hanging drops into bulk suspension cultures after 2-3 days of formation, whereby individual EBs tend to agglomerate. (wikipedia.org)
  • We previously showed that VEGF signaling in primary endothelial cell cultures caused overexpression of VEGFR2 in a positive feed-forward loop, which is at least partly regulated by loss of miRNA-mediated control of VEGF receptor degradation. (plos.org)
  • Dendrite branch point measurements in organotypic cultures Primary human MCs were infected with lentivirus expressing a myristoylated tdTomato construct to allow visualization of the MCs when incorporated into 3D organotypic cultures as described above. (biotech-angels.com)
  • The effect of TNF-α and IL-1β on NKCC1 expression in primary astrocyte cultures was determined. (biomedcentral.com)
  • Since Haberlandt's original assertions, methods for tissue and cell culture have been realized, leading to significant discoveries in biology and medicine. (wikipedia.org)
  • Dot blot analysis of cytokines Conditioned media from KCs was concentrated 5 by centrifugation through a Centricon Plus-70 Centrifugal Filter with a cutoff of ACTB 3 kDa (MilliporeSigma), according to the manufacturers instructions. (biotech-angels.com)
  • 7] Gram stains in bacterial infection pericardial fluid have a specificity of 99% but a sensitivity of only 38% compared with bacterial cultures. (medscape.com)
  • Purified 5,11,14 20:3 was taken up by cultured human skin keratinocytes, reduced 20:4n-6, and reduced PGE 2 levels dramatically. (biomedcentral.com)
  • Is 5,11,14 20:3 taken up by cultured human keratinocyte PL, can it displace 20:4n-6 from PL pools and reduce PGE 2 levels, and does it affect tumor necrosis factor α(TNFα) production? (biomedcentral.com)
  • In the pyrene and fluoranthene degradation experiment using a minimum medium, B. fungorum isolate no. 1 and M. gilvum isolate no. 13 were able to degrade 98.6% + 1.9 of pyrene after 20 days, and 99.9% ± 0.1 after a 16-day incubation period, respectively. (omicsonline.org)
  • The recalcitrant lignins, cellulose, phenols, fatty acids, tannins, and resins from materials are washed off as black liquor during the heating step (under high alkaline pH and temperature conditions). (ncsu.edu)
  • Under healthy conditions, the in- nomes obtained from individuals crobiota resembles the mother's skin testinal microbiota is considered to on three continents, including 760 microbial community, composed of be a stable community, influenced samples from Europe. (who.int)
  • Both conditions can be accompanied by decreased levels of heme oxygenase-1 (HMOX1), cytoprotective, heme-degrading enzyme. (hindawi.com)
  • High salt diets ( 1 , 2 ), renal impairment ( 3 - 6 ), inflammation, and infection ( 7 - 11 ) can induce Na + accumulation in skin tissues that can be simulated by addition of approximately 40 mM NaCl to standard cell culture media (= high salt condition, HS) ( 2 , 8 , 12 , 13 ). (frontiersin.org)
  • If bacterial infection is suspected, at least 3 cultures of pericardial fluid for aerobes and anaerobes as well as blood cultures are required. (medscape.com)
  • 15 years, P. orbiculare was not cultured the entire surface of the plate. (who.int)
  • This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. (aging-us.com)
  • In the cell culture, they can bind Ulex europaeus lectin and acetylated low-density lipoprotein (LDL) [ 2 ]. (hindawi.com)
  • Essential Pharma focused on filling in the hole in this cell culture void. (genengnews.com)
  • To facilitate cell culture optimization, Essential Pharma has developed Cell-Ess, a serum replacement that is free of animal-origin components. (genengnews.com)
  • Cell culture techniques were advanced significantly in the 1940s and 1950s to support research in virology . (wikipedia.org)
  • The injectable polio vaccine developed by Jonas Salk was one of the first products mass-produced using cell culture techniques. (wikipedia.org)
  • This vaccine was made possible by the cell culture research of John Franklin Enders , Thomas Huckle Weller , and Frederick Chapman Robbins , who were awarded a Nobel Prize for their discovery of a method of growing the virus in monkey kidney cell cultures. (wikipedia.org)
  • Strikingly, D-limonene addition in the fermentation medium repressed the production of polyunsaturated fatty acid (PUFAs). (mdpi.com)
  • These results strongly suggest the induction of necroptosis in response to GEF under AAS culture conditions. (spandidos-publications.com)
  • There are high function similarities between wild O. sinensis and cultured H. sinensis ,both of them be used to treat weakness after sickness, lung and kidney-associated diseases and sexual dysfunction (Buenz et al. (springeropen.com)
  • The microbiome, the "other ge- species have no representative in nome" or "second genome" of the culture collections. (who.int)
  • The presence of necrostatin-1, an inhibitor of receptor-interacting serine/threonine-protein kinase 1 (RIPK‑1), but not that of Z-VAD-fmk, attenuated the cytotoxic effects of GEF under AAS culture conditions. (spandidos-publications.com)
  • Roots did not form on any cuttings after four months and all cuttings were subsequently discarded. (aff.org.au)
  • Subsequently, a cascade of signaling events initiated by the preconditioning stress establishes delayed protection. (jneurosci.org)