• The Hep G2 cell line has been isolated from a liver biopsy of a male Caucasian aged 15 years, with a well differentiated hepatocellular carcinoma. (sigmaaldrich.com)
  • Chronic hepatitis B virus (HBV) and hepatitis D virus (HDV) infections are major causes of liver disease and hepatocellular carcinoma worldwide. (mdpi.com)
  • In this study we explored the underlying mechanisms of PFOA and GenX induced hepatocellular damage. (illinois.edu)
  • Liver hepatocellular carcinoma cell line HepG2 was used as a model to study induced liver inflammation in vitro at the cellular, genetic, and epigenetic levels. (illinois.edu)
  • mRNA expression analysis of PFOA exposed cells showed that cell cycle homeostasis genes were affected significantly, as well as the ten-eleven translocation methylcytosine dioxygenases (TETs) and the essential lipid metabolism genes. (illinois.edu)
  • Wen, Y, Mirji, N & Irudayaraj, J 2020, ' Epigenetic toxicity of PFOA and GenX in HepG2 cells and their role in lipid metabolism ', Toxicology in Vitro , vol. 65, 104797. (illinois.edu)
  • Plasminogen activator inhibitor-1 synthesis in the human hepatoma cell line Hep G2. (jci.org)
  • The aim of this study was to analyze the insulin-mediated response of PAI-1 and lipid synthesis in Hep G2 cells after producing an insulin-resistant state by decreasing insulin receptor numbers. (jci.org)
  • The results indicate that PAI-1 synthesis in presence of insulin is markedly increased in down-regulated cells, and that metformin inhibits this effect by acting at the cellular level. (jci.org)
  • Flow cytometry and western-blot further demonstrated that B7-H1 protein synthesis was enhanced in HBx(+)-HepG2 cells. (nih.gov)
  • ATF-3 induces DNA synthesis and expression of cyclin D1 in hepatocytes [ 21 ] and is involved in serum-induced cell proliferation as a target gene of c-myc [ 10 ]. (hindawi.com)
  • HP75 cells treated with overexpressed miR-543 exhibited increased cell proliferation, migration and invasion, while decreased cell apoptosis as well as expression of Cleaved caspase-3 and Cleaved caspase-8 were observed. (cancerindex.org)
  • Hep G2 cell line human has been used to study the cytotoxicity of carbon monoxide-releasing molecules and organic solvents on HepG2 cells and to evaluate the antiproliferative activity of aqueous extracellular polysaccharides (AEPS) for HepG2 using the MTT colorimetric assay. (sigmaaldrich.com)
  • MTT assay was employed to study the anticancer activity against cancer cell lines Hep-G2, MCF7 and normal VERO cell lines. (google.com)
  • Crude methanolic extract of G. corticata had significant anticancer activity followed by E. antenna and E. linza on cancer cell lines Hep-G2, MCF7 and normal VERO cell lines by MTT assay. (google.com)
  • 3. Subject has an APOL1 genotype of G1/G1, G2/G2, or G1/G2 obtained with a Vertex designated investigational clinical study assay. (who.int)
  • The major risk factors include chronic hepatitis B virus (HBV) infection, chronic hepatitis C virus (HCV) infection, environmental carcinogens such as aflatoxin B1 (AFB1), alcoholic cirrhosis, and inherited genetic disorder such as hemochromatosis, Wilson's disease, and tyrosinemia. (hindawi.com)
  • Preincubation by an 18-h exposure of Hep G2 cells to 10(-7) M insulin aimed at reducing the number of insulin receptors, was followed by a subsequent 24-h stimulation with 10(-9) M insulin. (jci.org)
  • Global methylation levels of HepG2 cells were found to be inversely proportional to PFOA exposure levels. (illinois.edu)
  • MicroRNA-543 promotes cell invasion and impedes apoptosis in pituitary adenoma via activating the Wnt/β-catenin pathway by negative regulation of Smad7. (cancerindex.org)
  • Co-culture experiments with HBx(+)-HepG2/T cells showed that the number of apoptotic T cells increased profoundly, and this effect could be partially prevented when a neutralizing mAb against B7-H1 was added to the culture, demonstrating that B7-H1 signaling can promote T cell apoptosis. (nih.gov)
  • Our results suggest that the expression of B7-H1 in hepatocarcimona cells can be initiated by HBx antigen, thus inducing T cell apoptosis and finally potentially facilitates the genesis of HCC. (nih.gov)
  • Activating transcription factor-(ATF-) 3, a stress-inducible transcription factor, is rapidly upregulated under various stress conditions and plays an important role in inducing cancer cell apoptosis. (hindawi.com)
  • First, we found that GS-002 significantly inhibited cell proliferation and induced cell apoptosis in dose-dependent manners. (hindawi.com)
  • Furthermore, we found that GS-002 induced more cell apoptosis in ATF-3-overexpressing cells. (hindawi.com)
  • The cells will respond to stimulation with human growth hormone. (sigmaaldrich.com)
  • In this study, in order to identify the reactive metabolite of NVP mainly responsible for CYP inhibition and liver injury, we synthesized five NVP analogs designed to avoid the proposed bioactivation pathway and evaluated their metabolic stabilities, CYP3A4 time-dependent inhibitory activities, and cytotoxicity. (unboundmedicine.com)
  • A large number of studies have demonstrated that the synergistic collaboration of a number of microRNAs (miRNAs), their growth factors and their downstream agents is required for the initiation and completion of pathogenesis in the liver. (wjgnet.com)
  • Hepatitis B virus surface antigens have not been detected. (sigmaaldrich.com)
  • While the molecular biology of the two viruses has been characterized in great detail, the absence of robust cell culture models for HBV and/or HDV infection has limited the investigation of virus-host interactions. (mdpi.com)
  • The cross-talk between the hepatitis B virus X protein (HBx) and B7-H1 in hepatocarcinoma (HCC) is unclear. (nih.gov)
  • Hep G2 cells may be a suitable model to study PAI-1 regulation in response to hyperinsulinemia. (jci.org)
  • 1998. Protection of human upper respiratory tract cell lines against sulphur mustard toxicity by hexamethylenetetramine (HMT). (cdc.gov)
  • One of human HCC cell lines, HepG2 cells, was transfected to stably express HBx protein (HBx(+)-HepG2). (nih.gov)
  • In this study, we examined the antitumor effects of GS-002 in human hepatoma Hep3B and HepG2 cells in vitro . (hindawi.com)
  • The induction of ATF-3 expression was mediated by mitogen-activated protein kinase (MAPK) signaling pathways in GS-002-treated cells. (hindawi.com)
  • In paper IV, we show that allele substitution at rs2289669 and rs8065082 does not affect SLC47A1 expression or splicing in HepG2 cells. (lu.se)
  • Several main apoptotic indicators were found in GS-002-treated cells, such as the cleaved forms of caspase-3, caspase-9, and poly(ADP-ribose) polymerase (PARP). (hindawi.com)
  • The pyrimidine analog of NVP did not induce cytotoxicity in HepG2 and transchromosomic HepG2 cells, expressing major four CYP enzymes and CYP oxidoreductase. (unboundmedicine.com)
  • The transcription of B7-H1 mRNA was increased significantly in these cells compared to cells transfected with control vector (HBx(-)-HepG2), as confirmed by a comparative genome-wide microarray analysis (Capitalbio) and real time quantitative PCR (qPCR). (nih.gov)
  • In breast cancer, ATF-3 enhances cancer cell-initiating features [ 22 ] and is associated with activation of the canonical Wnt/ β -catenin pathway [ 23 ]. (hindawi.com)
  • B. U-373, SW-480 und Hep-G2, A-431, MCF-7, PC-3) wurde Kanadaptin sowohl auf Protein- als auch auf Transkriptionsebene (RT-PCR) nachgewiesen. (uni-wuerzburg.de)
  • 2000. Reactivity of chloroethyl sulfides in the presence of a chlorinated prophylactic: a kinetic study by EPR/spin trapping and NMR techniques. (cdc.gov)
  • To address those questions, we applied functional genomics approaches to study genetic variants pertinent to obesity and type 2 diabetes-related traits. (lu.se)
  • The cells secrete a variety of major plasma proteins e.g. albumin, α2-macroglobulin, α 1-antitrypsin, transferrin and plasminogen. (sigmaaldrich.com)
  • The aim of the study was to investigate the bioactive properties of three seaweed samples, Enteromorpha antenna, Enteromorpha linza and Gracilaria corticata were collected from the shoreline of Mahabalipuram, Tamilnadu. (google.com)
  • In this study, fatty acids -treated HepG2 cells and orotic acid -treated rats were used to investigate the lipid -lowering effects of sea cucumber saponin derivatives. (bvsalud.org)
  • 1. History of any illness or any clinical condition that, in the opinion of the investigator, might confound the results of the study or pose an additional risk in administering study drug(s) to the subject. (who.int)
  • The present study was conducted with aim of investigating the role of microRNA-543 (miR-543) in PA development. (cancerindex.org)
  • GWAS studies have identified hundreds of loci that associate with obesity and type 2 diabetes. (lu.se)
  • Previous studies reported that ATF-3 was induced by treating cells with antitumorigenic compounds [ 14 - 18 ] and a phosphoinositide 3-kinase inhibitor [ 19 ]. (hindawi.com)
  • This study analyzed the potential relationships between HBx and B7-H1 in hepatocarcinogenesis. (nih.gov)
  • By documenting the traditional uses and biological activities of Moringa species, we hope to support new research on these plants, especially on those species whose biological properties have not been studied to date. (frontiersin.org)