Studies conformational protein protein proteins magnetic resonance spectroscopy. Medical search. Frequent questions

My group exploits the complementary nature and distance dependence of nuclear magnetic resonance (NMR) and single molecule fluorescence, in particular single molecule Förster Resonance Energy Transfer (FRET), to study the conformational landscape and dynamics of IDPs at molecular resolution. (fu-berlin.de)
In CONCERT we will develop a method based on the use of nuclear magnetic resonance spectroscopy in solution that will provide very detailed descriptions of such changes by using the information about structural heterogeneity contained in a parameter that is exquisitely sensitive to molecular shape called residual dipolar coupling measured in steric alignment. (europa.eu)
To complement the structural information obtained by nuclear magnetic resonance and, especially, measure the rate of information transfer across the androgen receptor we will carry out in a collaborative fashion high precision single molecule Förster resonance energy transfer and fluorescence correlation spectroscopy experiments on AR constructs labelled with fluorescent dyes. (europa.eu)
Our lab studies the structure and dynamics of proteins, protein-protein complexes, and protein-nucleic acid complexes using multidimensional nuclear magnetic resonance (NMR) spectroscopy, and we develop and apply novel NMR and computational methods to aid in these studies. (nih.gov)
Our studies rely on nuclear magnetic resonance (NMR) spectroscopy, a research approach that relies on the magnetic properties of the nucleus of certain atoms to determine physical and chemical properties of the molecules in which they are contained. (nih.gov)
Nuclear magnetic resonance spectroscopy, Radiolabeling, Biomolecules-Analysis, Isotope Labeling-methods, Nuclear Magnetic Resonance, Biomolecular Here we describe a protocol for the labeling of proteins that facilitates their study using a technique that is sensitive to millisecond conformational exchange restrict to techniques of labeling with isotopes (stable or radioactive): do not use for radioisotope tracer studies of "labeled" cells, microorganisms or chemicals Pris: 1862 kr. (firebaseapp.com)
To gain an atomic-detail description of these processes and to characterize novel therapeutic targets, we integrate cutting-edge structural biology methods from Nuclear Magnetic Resonance Spectroscopy, complementary biophysical and biochemical techniques, computational modeling and functional immunoassays. (upenn.edu)
Via mutagenesis, biophysical studies, and Nuclear Magnetic Resonance spectroscopy (NMR), we study A2AR conformational dynamics in response to drugs, to understand the role of microswitches in GPCR signalling - addressing questions of energetics, efficacy, biased signaling, allostery via cations and membrane adjuvants, and cooperative dynamics in the associated G protein. (cuny.edu)
Using a technique called nuclear magnetic resonance spectroscopy, Fong et al. (elifesciences.org)
In this chapter, we describe the use of high-pressure coupled to Nuclear Magnetic Resonance (NMR) spectroscopy to reveal unprecedented details on the folding energy landscape of proteins. (pasteur.fr)
In my research group we primarily use nuclear magnetic resonance (NMR) spectroscopy to study protein dynamics over a wide range of time scales, from picoseconds to seconds. (lu.se)
An overview of the metabolic differences between Bradyrhizobium japonicum 110 bacteria and differentiated bacteroids from soybean (Glycine max) root nodules : an in vitro C- and P-nuclear magnetic resonance spectroscopy study. (ibs.fr)
Hydrogen-deuterium exchange mapping of Abeta 1-40 amyloid fibril secondary structure using nuclear magnetic resonance spectroscopy. (jnu.ac.in)
Two proteins putatively involved in the persistence of Mycobacterium tuberculosis infection have been investigated by heteronuclear nuclear magnetic resonance (NMR) spectroscopy and other methods. (ndltd.org)
Nuclear Magnetic Resonance ( NMR ) spectroscopy is a powerful tool for the study of structural biology, structure/activity relationships and macromolecule and small-molecule analysis. (irbm.com)
Our principal experimental tool is nuclear magnetic resonance (NMR), in particular nuclear spin relaxation measurements. (lu.se)
This is normally associated with fast dissociation rates of the corresponding complexes, in turn leading to fast exchange on the nuclear magnetic resonance (NMR) chemical shift time scale and on the NMR relaxation time scale. (uni-luebeck.de)
Whereas transferred nuclear Overhauser effect (NOE) experiments deliver bioactive conformations of carbohydrates binding to proteins, saturation transfer difference (STD) NMR spectra provide binding epitopes and valuable information about the binding thermodynamics and kinetics. (uni-luebeck.de)
These so-called FYRN and FYRC motifs are also found in transforming growth factor beta regulator 1 (TBRG1)/nuclear interactor of ARF and MDM2 (NIAM), a growth inhibitory protein that also plays a role in maintaining chromosomal stability. (diamond.ac.uk)
In particular, they are and nuclear magnetic resonance (NMR) refinement. (lu.se)

The binding of αKG and substrate has been analyzed by X-ray crystallography, molecular dynamics calculations, and NMR spectroscopy. (wikipedia.org)
The purpose of my lab's research is to understand the interrelationship between the structure, dynamics, and function of proteins. (nih.gov)
Our lab is developing new tools and techniques that facilitate the study of the structure and dynamics of proteins and protein complexes, functional units that include one or more proteins. (nih.gov)
To date, most dynamics studies have been performed using uniform 15 N enrichment. (firebaseapp.com)
Experimental methods of multi-dimensional NMR spectroscopy, dynamics and theoretical description of spin system evolution, multiple resonance NMR technique for structural studies of proteins and nucleic acids by NMR spectroscopy, contemporary methods of structural biology, studies of complex biomolecular systems. (muni.cz)
These include characterizing the structural, thermodynamic and dynamic properties of the barriers between conformational states on the protein energy landscape, understanding the sequence dependence of folding cooperativity, defining more clearly the role of solvation in controlling protein stability and dynamics and probing the high energy thermodynamic states in the native state basin and their role in misfolding and aggregation. (pasteur.fr)
I am interested in how protein dynamics contribute to biological function. (lu.se)
We address specific questions regarding the role of protein dynamics in various types of function and dysfunction, for example: ligand binding and allostery, enzyme catalysis, and protein misfolding and aggregation. (lu.se)
Change of dynamics of raft-model membrane induced by amyloid-beta protein binding. (ibs.fr)
The ecology of protein dynamics. (ibs.fr)
The scope of the systems covered ranges from cellular to tissue, organ, systems studies, and up to populational dynamics. (nih.gov)
This includes gene, protein and metabolic networks, cellular architecture and intracellular dynamics, cell communication and motility, cell division and differentiation, tissue formation and organogenesis, tissue and organ functions, changes in population characteristics as a consequence of interaction of organisms with their physical environment, with individuals of their own species, and with organisms of other species. (nih.gov)
The combination of these diverse methods contributes to a holistic understanding of the intricate dynamics and functions of these signaling proteins. (drgpcr.com)
Quantitative studies of biomolecular interactions, dynamics and structure at the molecular level establish the necessary foundation for analyzing higher levels of biological complexity. (lu.se)
Quasielastic incoherent neutron scattering (QENS) is a well-suited experimental method to study protein dynamics from the picosecond to several nanoseconds and in the Ångström length-scale. (lu.se)
In my presentation, I will summarize recent QENS and NSE results on the dynamics of the intrinsically disordered myelin basic protein (MBP) and the chemically denatured bovine serum albumin (BSA) (1,2,3). (lu.se)
For metal- powerful methods to obtain and compare reaction and activation loproteins, a third method to obtain local information about the energies for suggested enzyme mechanisms and they can also provide atomic details about the protein dynamics. (lu.se)

The class-I and class-II proteins of the Major Histocompatibility Complex play an essential role in immune surveillance, by displaying epitopic peptides derived from the processing of aberrant proteins on the cell surface, where they can be recognized through interactions with specialized receptors on T cells and Natural Killer cells. (upenn.edu)
The reason why Peter Lansbury and others used the term 'natively unfolded' for a-synuclein and other proteins, especially peptides, is that they do not exist, to a significant degree, in the same kind of stable structure that larger native proteins like myoglobin do. (alzforum.org)
As generation of site-specific PTMs in proteins is challenging, we used short synthetic peptides to quantify the effects on tubulin binding of three site-specific PTMs located within the PHF6∗ (paired helical filament [PHF] residues 275-280) and PHF6 (residues 306-311) hexapeptide motifs: K280 acetylation, Y310 phosphorylation, and K311 succinylation. (bvsalud.org)
Bioinformatic algorithms allowing predicting of biomolecular folding for proteins, peptides, and RNAs, even though sometimes successful, have all their limitations. (biosyn.com)
RNA molecules in solution may adopt secondary structures that are only partially determined by thermodynamics since RNA molecules can undergo conformational changes during interaction with other RNAs, RNA binding proteins or RNA binding peptides. (biosyn.com)
To support our discovery programs we can use CD spectroscopy for different applications for the study of secondary structure content of peptides and proteins, monitoring conformational transitions , and the study of the thermodynamics of unfolding and protein-ligand interactions . (irbm.com)
The fast and real time analysis of the kinetic and thermodynamic binding parameters of small molecules , peptides , antibodies , or other protein-protein interactions, are crucial information for target characterization, hit identification, lead optimization or characterization of the binding properties of a molecule to its target. (irbm.com)
Apelin peptides are cognate ligands for the apelin receptor, a G-protein-coupled receptor (GPCR). (phoenixpeptide.com)
This 1H NMR invisible matrix was used for the solid-phase synthesis of peptides, oligoureas, and a series of amides as well as their characterization by HRMAS NMR spectroscopy. (cnrs.fr)
A series of [L-(alphaMe)Leu]n (n = 1-5) homo-peptides have been covalently linked to Tentagel and POEPOP resins and submitted to a conformational study using HRMAS NMR spectroscopy. (cnrs.fr)

Their dynamic features and broad range of interaction modes, however, render them difficult to study, and analyzing their complexes often requires integratedapproaches. (fu-berlin.de)
A particular focus is the study of rare, highly transient, "excited" states of proteins and their complexes that play a key role of molecular recognition. (nih.gov)
We are particularly interested in complexes involved in signal transduction and transcriptional regulation, and on AIDS and AIDS-related proteins. (nih.gov)
The many proteins and protein complexes we describe are resulting in new insights into fundamental cell operations. (nih.gov)
Our lab's principal efforts focus on mechanistic, structural and functional studies of protein complexes, with emphasis on the development of an iterative experimental / computational approach. (upenn.edu)
However, removing RbmA led to visually similar biofilms, despite the absence of the cross-linked protein complexes. (elifesciences.org)
However, structural studies on serpin-peptidase complexes reveal a broader set of contacts on the scaffold of inhibitory serpins that have substantial influence on guiding peptidase recognition. (cnrs.fr)
Over the last decades, a variety of RNA methods have been developed for the study of RNA-DNA, RNA-RNA, and RNA-protein interactions including RNA complexes with ligand molecules. (biosyn.com)
Exploration of the supramolecular interactions involving tris-dipicolinate lanthanide complexes in protein crystals by a combined biostructural, computational and NMR study. (ibs.fr)
The formation of functional complexes involving GPCRs and β-arrestins hinges on their specific conformational states, influenced by their intricate three-dimensional structures. (drgpcr.com)
We demonstrate the power of a combined transfer NOE/STD NMR approach for the analysis of carbohydrate-protein complexes using selected examples. (uni-luebeck.de)
Protein complexes, which are formed as a result of these interactions, consist of two or more components that associate along specific pathways - protein association pathways. (lu.se)

Such states, which are invisible to conventional biophysical techniques, including crystallography, play a critical role in macromolecular recognition, allostery induced fit, conformational selection, and molecular assembly. (nih.gov)
My research focuses on understanding the intricate molecular mechanisms that determine the vast repertoire of peptide antigens displayed by the proteins of the Major Histocompatibility Complex for immune surveillance by T cells and Natural Killer cells. (upenn.edu)
Our detailed biophysical characterization of the molecular chaperone TAPBPR provided us with a high-resolution view of peptide exchange on MHC-I proteins and led to the development a high-throughput methodology for generating barcoded MHC tetramer libraries encompassing hundreds of unique antigen specificities. (upenn.edu)
See also David Thirumalai's Emerging Ideas on the Molecular Basis of Protein and Peptide Aggregation ' (.pdf). (alzforum.org)
Since RNA-binding proteins (RBPs) are key players in the post-transcriptional regulation of gene expression precise knowledge of their binding sites is critical for determining their molecular function and for understanding their roles in cell development and disease. (biosyn.com)
Because most receptor proteins to which carbohydrates bind are rather large with molecular weights significantly exceeding 30 kDa, the analysis of the corresponding protein spectra is not trivial, and only very few studies have been addressing this issue so far. (uni-luebeck.de)
Protein structures are invaluable for biochemical studies and for mechanical or molecular mechanics calculations have become the understanding of how proteins work. (lu.se)

Qualitative and quantitative approaches are undertaken to effectively integrate parameters from both techniques to shed new light onto how IDPs and their interactions with folded proteins regulate and enable various biological processes. (fu-berlin.de)
As the assembly was carried out in the absence of ATP, this suggests that formation of the holoenzyme from the individual subcomplexes is solely driven by protein-protein interactions at the interface. (upstate.edu)
Unlike reversible disassembly, which requires ATP hydrolysis on V 1 to break protein-protein interactions, Oxr1p mediated disassembly of V-ATPase is ATP independent and therefore a novel mode of activity regulation. (upstate.edu)
Impact of nucleic acid self-alignment in a strong magnetic field on the interpretation of indirect spin-spin interactions. (muni.cz)
The mapping of RNA-protein or RNA-RNA interactions by protein pull-down or affinity pull-down methods allow studying RNA structures, as well as RNA-protein, and RNA-RNA interactions. (biosyn.com)
Carbohydrate-protein interactions are frequently characterized by dissociation constants in the μM to mM range. (uni-luebeck.de)
Therefore, NMR experiments that take advantage of fast exchange are well suited to study carbohydrate-protein interactions. (uni-luebeck.de)
Two types of NMR experiments have been extremely valuable to analyze carbohydrate-protein interactions at atomic resolution. (uni-luebeck.de)
In addition, we will have regular interactions with the permanent staff at Physical Chemistry and will get to meet students in their fi nal studies thereby enlarging our network and creating possibilities for future recruitment. (lu.se)

Many assays were developed to study αKG-dependent dioxygenases so that information such as enzyme kinetics, enzyme inhibition and ligand binding can be obtained. (wikipedia.org)
For example, the rhodopsin (class A) family, encompassing ~700 GPCRs, possesses a common network of "microswitches" that extend from the ligand binding pocket to the G protein binding interface. (cuny.edu)
Our research has resulted in unique insights showing how conformational entropy can contribute significantly to ligand binding and allostery, which has future applications in the field of drug design. (lu.se)
RNA molecules are functionally diverse and involved in many cellular processes such as catalysis, ligand binding, and protein recognition. (biosyn.com)
In this study, the long binding helix of this ligand was downsized to an enzymatically stable cyclic peptide endowed with sub-nanomolar binding affinity toward the αvβ6 receptor and remarkable selectivity against other integrins. (cipsm.de)
In general, it is possible to analyze ligand binding by observing either protein signals or ligand resonances. (uni-luebeck.de)

The Department Structure and Function of Proteins uses protein crystallography to investigate proteins that play a role in infectious disease, e.g. by controlling the production of toxic molecules or by acting as toxins themselves. (helmholtz-hzi.de)
Cryo-EM, on the other hand, requires less protein and has evolved to achieve resolutions comparable to X-ray crystallography ( García-Nafría, J., & Tate, C. G. 2021 ). (drgpcr.com)
The most common strong alternative and complement to experimental methods to methods to obtain protein structures are X-ray crystallography obtain insight in biochemical processes. (lu.se)

CD is a powerful tool for studying peptide and protein conformation requiring a minimal amount of sample compared to other techniques. (irbm.com)
The infectious agent in the prion disease is composed mainly or entirely of an abnormal conformation of a host-encoded glycoprotein called the prion protein. (medscape.com)
The replication of prions involves the recruitment of the normally expressed prion protein, which has mainly an alpha-helical structure, into a disease-specific conformation that is rich in beta-sheet. (medscape.com)

The scope of studies ranges from those focused-on sequencing, mass spectrometry, bioimaging and other high-through-put techniques in data to medicine, pharmacology, and populational studies. (nih.gov)
In biochemical studies, mass spectrometry is used for enzyme activity measurement by product/substrate detection or determination or for the measurement of covalent binding . (irbm.com)

volume = {285}, number = {32}, pages = {24307--24312}, abstract = {Inhibitory serpins are metastable proteins that undergo a substantial conformational rearrangement to covalently trap target peptidases. (cnrs.fr)
Another key result is the critical role of rare, transient, high-energy conformations in biological function: proteins spontaneously undergo transitions between multiple structural states that are either active or inactive. (lu.se)
Zinc-binding motifs are stable structures, and they rarely undergo conformational changes upon binding their target. (embl.de)

Interleukin-2 druggability is modulated by global conformational transitions controlled by a helical capping switch. (upenn.edu)
These types of transitions can mediate allosteric signaling within proteins via distinct pathways that we have detected using NMR spectroscopy. (lu.se)

Genetic studies in Arabidopsis implicate an α/β-hydrolase, KARRIKIN-INSENSITIVE 2 (KAI2) as a receptor for karrikins, germination-promoting butenolide small molecules found in the smoke of burned plants. (nih.gov)
a protein required for the differentiation of the set of six touch receptor neurons in this nematode. (embl.de)
Assessing the conformational changes of pb5, the receptor-binding protein of phage T5, upon binding to its Escherichia coli receptor FhuA. (ibs.fr)

In recent work, our team found that the dynamic plasticity of MHC-I proteins, occurring over a range of timescales from microseconds to milliseconds, plays an essential role in their peptide selector function though the sampling of minor, "excited-state" conformations. (upenn.edu)
Two peptide fragments, corresponding to the amino acid residues 106-126 (PrP[Ac-106-126-NH2]) and 106-114 (PrP[Ac-106-114-NH2]) of the human prion protein have been synthesised in the acetylated and amide form at their N- and C-termini, respectively. (cnr.it)

When we're taught biochemistry, we see proteins in their physiologically active forms. (alzforum.org)

These amyloid plaques are immunoreactive with antibodies to the prion protein and do not immunoreact with antibodies to other amyloidogenic proteins, such as the amyloid-beta (which is deposited in Alzheimer disease). (medscape.com)

Changes in the redox state of cells affect proteins, lipids, and nucleic acids in different ways. (hindawi.com)
Highly divergent hypotheses have been put forward regarding the makeup of the prions, including that they consist of nucleic acid only or protein only, are lacking both protein and nucleic acid, or are a polysaccharide. (medscape.com)

This work highlights a new approach for studying minor conformational changes due to structural plasticity within a single dimeric interface in solution. (umn.edu)
Consideration of related TPx crystal structures indicates that the missing cross peaks correspond to a region of the protein that must display conformational plasticity necessary for the mechanism of action suggesting that the spectrum is affected by conformational exchange processes. (ndltd.org)

Multidimensional heteronuclear NMR spectroscopy was applied to recombinant wild-type (Wt) and Cys60→Ser (C60S) TPx, both of which are clearly folded but display slightly different spectral characteristics. (ndltd.org)

A recent study from the Wilkens lab in collaboration with Seoul National University uncovered a novel mechanism of yeast V-ATPase regulation based on biochemical experiments and cryo electron microscopy (cryo-EM) structures of holo V-ATPase and Oxr1 bound V 1 subcomplex. (upstate.edu)

Some LIM domains bind protein partners via tyrosine-containing motifs. (embl.de)
Zinc finger (Znf) domains are relatively small protein motifs which contain multiple finger-like protrusions that make tandem contacts with their target molecule. (embl.de)
They display considerable versatility in binding modes, even between members of the same class (e.g. some bind DNA, others protein), suggesting that Znf motifs are stable scaffolds that have evolved specialised functions. (embl.de)
Many chromatin-associated proteins contain two sequence motifs rich in phenylalanine/tyrosine residues of unknown function. (diamond.ac.uk)

Triple resonance NMR experiments recorded using 2H, 13C, 15N -labelled C60S yielded assignments for the majority of the backbone resonances. (ndltd.org)
From CD experiments at different SDS concentrations, an alpha-helix/beta-sheet conformational transition was only observed in the blocked PrP[Ac-106-126-NH2] sequence. (cnr.it)
Perturbation of this nature was also observed in magnetically aligned bicelles by 31P solid-state NMR spectroscopy and spin relaxation experiments. (phoenixpeptide.com)
In QENS experiments of protein solutions hydrogens act as reporters for the motions of methyl groups or amino acids to which they are bound. (lu.se)

Efficient refolding of aggregation prone Citrate Synthase by polyol osmolytes: How well are protein folding and stability aspects coupled? (jnu.ac.in)
Interestingly, these variable regions determine the differential effects of Hsp70 isoforms on the aggregation or degradation of the Alzheimer's-disease-related protein tau [ 16 ] . (encyclopedia.pub)

Computational design of closely related proteins that adopt two well-defined but structurally divergent folds. (upenn.edu)
In general, the computational load rises rapidly with the number of atoms and electrons considered and therefore, only rather small systems can be studied accurately. (lu.se)

Although both the hydrophobic aliphatic chain and hydrophilic ζ -amino group of the Lys side chain presumably contribute to the structures and functions of proteins, the dual nature of the Lys residue has not been fully investigated using NMR spectroscopy, due to the lack of appropriate methods to acquire comprehensive information on its long consecutive methylene chain. (copernicus.org)
Despite their contributions, these methods often lack cellular auxiliary structures and proteins. (drgpcr.com)
Structures of a light-oxygen-voltage paradigm at around 1 Å resolution revealed highly similar light-induced conformational changes, irrespective of whether the glutamine is present. (nature.com)
CD spectra in the far UV range report for secondary structures while in the near UV they are characteristics of the tertiary structure of proteins. (irbm.com)

Labeling occurs by site-specific incorporation of stable isotope labeled tags at cysteine residues or the general labeling of amines and carboxyl groups in protein samples. (firebaseapp.com)

Structural and biophysical studies also reveal how aberrant serpin folding can lead to the formation of domain-swapped serpin multimers rather than the monomeric metastable state. (cnrs.fr)

However, efficient labeling of proteins with selected amino acids necessitates auxotrophic hosts, which are often not available. (firebaseapp.com)
Rhombotin 1 (RBTN1 or TTG-1) and rhombotin-2 (RBTN2 or TTG-2) are proteins of about 160 amino acids whose genes are disrupted by chromosomal translocations in T-cell leukemia. (embl.de)

Hsp70, Hsp90, and their co-chaperones are crucial members of the proteostasis network that are able to recognize misfolded proteins, aberrant condensates and protein aggregates, triaging proteins for refolding or degradation. (encyclopedia.pub)

The degree of conformational order in the prefibrillar aggregates is not yet known, I don't think. (alzforum.org)

In mammals and humans, αKG-dependent dioxygenase have functional roles in biosyntheses (e.g. collagen biosynthesis and L-carnitine biosynthesis), post-translational modifications (e.g. protein hydroxylation), epigenetic regulations (e.g. histone and DNA demethylation), as well as sensors of energy metabolism. (wikipedia.org)
12. C. This gives a Amino-acid selective isotope labeling of proteins offers numerous advantages in mechanistic studies by revealing structural and functional information unattainable from a crystallographic approach. (firebaseapp.com)

GPCR signaling is a complex process modulated by protein conformational states. (drgpcr.com)

Mammalian cysteine-rich intestinal protein (CRIP), a small protein which seems to have a role in zinc absorption and may function as an intracellular zinc transport protein. (embl.de)

Limited chemical shift dispersion represents a significant barrier to studying multistate equilibria of large membrane proteins by 19F NMR. (bvsalud.org)
To investigate the reaction catalysed by IdmH, 88% of the backbone NMR resonances were assigned, and using chemical shift perturbation of [ 15 N]-labelled IdmH it was demonstrated that indanomycin binds in the active-site pocket. (iucr.org)

Zn2+ binding to the Met66 and Val66 prodomains results in distinct conformational and macroscopic differences observed by NMR, light scattering and cryoEM. (bvsalud.org)

Human brain derived neurotrophic factor (BDNF) encodes a protein product consisting of a C-terminal mature domain (mature BDNF) and an N-terminal prodomain, which is an intrinsically disordered protein. (bvsalud.org)

Chemical chaperone mediated protein folding: Stabilization of P22 tailspike folding intermediates by glycerol. (jnu.ac.in)

HMGB1 is an abundant protein, 10 6 molecules per cell [ 7 ], which has been postulated as a redox sensor [ 8 ]. (hindawi.com)
As measured by proton HRMAS of the swollen polymer, the signal intensity of the oxyethylene protons is reduced by a factor of 110 relative to the corresponding nondeuterated poly(oxyethylene)poly(oxypropylene) (POEPOP) resin, thus facilitating detailed HRMAS NMR studies of covalently linked molecules. (cnrs.fr)
The complete absence of the parasite resin signals will be of great advantage, for example, for the optimization of multistep solid-phase stereoselective reactions, and for the conformational study of resin-bound molecules in a large variety of solvents. (cnrs.fr)

Small angle neutron scattering for the study of solubilised membrane proteins. (ibs.fr)
Combining small-angle neutron and X-ray scattering for studying protein denaturation. (ibs.fr)
Combining NMR and small angle X-ray and neutron scattering in the structural analysis of a ternary protein-RNA complex. (ibs.fr)
The similar problem is faced when resolving conformational ensembles from small angle scattering data. (lu.se)

These extremely dynamic proteins are highly flexible and easily adaptable to different binding partners, making them important players in many biological processes, often with vital regulatory functions. (fu-berlin.de)
De novo design of a homo-trimeric amantadine-binding protein. (upenn.edu)
KAR1 binding induces a conformational change in KAI2 at the active site entrance. (nih.gov)
Finally, recent structural studies reveal how the serpin fold has been adapted for non-inhibitory functions such as hormone binding. (cnrs.fr)
To determine if Zn2+ mediated conformational change in the Met66 prodomain is required for biological effect, we mutated His40, a Zn2+ binding site, and observed a loss of Met66 prodomain bioactivity. (bvsalud.org)
Vertebrate insulin gene enhancer binding protein isl-1. (embl.de)
Isl-1 binds to one of the two cis-acting protein-binding domains of the insulin gene. (embl.de)
Lysozyme amyloidogenesis is accelerated by specific nicking and fragmentation but decelerated by intact protein binding and conversion. (jnu.ac.in)

For example, alkaline vacuole/lysosome are deficient in autophagy, Golgi pH regulates its ability to glycosylate proteins and failure to maintain endosomal pH perturbs with its ability to recycle receptors to the Plasma membrane or, the trans-Golgi. (upstate.edu)
The improved conformational sensitivity and line shape enable the detection of previously unresolved states in one-dimensional (1D) 19F NMR spectra of a 134 kDa membrane transporter. (bvsalud.org)

1 Jack H. Skirball Center for Chemical Biology and Proteomics, Salk Institute for Biological Studies, La Jolla, CA 92037, USA. (nih.gov)
Protein performs its biological functions by interacting with other proteins. (lu.se)

The purpose of this report is to describe the study design and methodological issues emerging from this pilot work relevant for the follow-up of this large birth cohort. (cdc.gov)

Neutron Spin-Echo spectroscopy (NSE) on the other hand, offers the highest energy resolution in the field of neutron spectroscopy and allows the study of slow collective motions in proteins up to several hundred nanoseconds and in the nanometer length-scale. (lu.se)

Intrinsically disordered proteins (IDPs) lack clearly defined three-dimensional structure and sample many different conformations on a sub-microsecond time scale. (fu-berlin.de)
Oligomeric proteins are important targets for structure determination in solution. (umn.edu)
While in most cases the fold of individual subunits can be determined experimentally, or predicted by homology-based methods, protein-protein interfaces are challenging to determine de novo using conventional NMR structure determination protocols. (umn.edu)
This would fundamentally change our conventional thinking about protein structure-function relationship, no? (alzforum.org)
I do not think there is a contradiction to the conventional thinking of protein structure-function relation. (alzforum.org)
However, it is still unclear how these matrix proteins interact to simultaneously remain a connected and flexible multicellular structure. (elifesciences.org)
To understand V-ATPase's role in health and disease, the Wilkens lab studies the structure and mechanism of the enzymes from yeast and human. (upstate.edu)
During protein synthesis, ribosomes become stalled on polyproline-containing sequences, unless they are rescued in archaea and eukaryotes by the initiation factor 5A (a/eIF-5A) and in bacteria by the homologous protein EF-P. While a structure of EF-P bound to the 70S ribosome exists, structural insight into eIF-5A on the 80S ribosome has been lacking. (cipsm.de)

Circular dichroism (CD) spectroscopy is a measurement of the difference in absorbance of right and left-circularly polarized light. (irbm.com)

The prime result of theoretical calculations is the energy of the studied system. (lu.se)

Mammalian and avian cysteine-rich protein (CRP), a 192 amino-acid protein of unknown function. (embl.de)

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