Structural proteins protein conformation. Medical search. Frequent questions

Using complementary approaches, i.e. controlled enzymatic hydrolysis and production of recombinant fusion proteins, we demonstrated that, for these patients, the allergenicity of the caprine β-casein could be abolished by substituting five amino acids residues. (cea.fr)

But trying to capture the conformations of proteins using X-ray crystallography, which was the standard methodology back then, frustrated him. (rockefeller.edu)
You will find out about the experimental techniques, such as X-ray crystallography, nuclear magnetic resonance and electron microscopy, which are used to determine the 3D structure of proteins. (manchester.ac.uk)
Small molecule ligand binding to the ATAD2 bromodomain is investigated here through the synergistic combination of molecular dynamics and protein crystallography. (rcsb.org)

however, a detailed understanding of effects of specific residues upon beta-turn stability and conformation is lacking. (rcsb.org)
As such, they represent a useful system with which to study the role that turn sequences play in determining structure, stability, and folding of the protein. (rcsb.org)
Two turns related by the threefold structural symmetry, the beta4/beta5 and beta8/beta9 turns, were subjected to both sequence-swapping and poly-glycine substitution mutations, and the effects upon stability, folding, and structure were investigated. (rcsb.org)
Stability, dynamics and interactions of proteins: packing and electrostatics. (lu.se)
This review critically focuses on opportunities to employ protein-graphene oxide structures either as nanocomposites or as biocomplexes and highlights the effects of carbonaceous nanostructures on protein conformation and structural stability for applications in tissue engineering and regenerative medicine. (mdpi.com)
We have found that although protein stability can be altered by single amino acid substitution, evolution for optimal function requires more subtle and delocalized mechanisms.Recent results implicate structure in the unfolded state as playing an important and novel role for the thermostability of these proteins. (berkeley.edu)
13 , 14 However, the amino acid perfectly maintains the stability of the mutual structural conformation of the virus S-protein and the ACE2 receptor in a holistic manner. (medsci.org)
Adsorption by electrostatic interaction of proteins in nanopores of an optimal size provides a favorably confining and protecting environment, which may lead to considerably enhanced structural stability and catalytic activity. (rsc.org)
Select appropriate experimental techniques for protein purification and characterization (conformation and stability), and describe the relative precision of various approaches and the assumptions implied by each method. (manchester.ac.uk)

born 9 May 1947) is a South African-born biophysicist and a professor of structural biology at Stanford University, a position he has held since 1987. (wikipedia.org)
Levitt was a PhD student in Computational biology at Peterhouse, Cambridge, and was based at the Laboratory of Molecular Biology from 1968 to 1972, where he developed a computer program for studying the conformations of molecules that underpinned much of his later work. (wikipedia.org)
To carry out their activities, Research Teams of the Frédéric Joliot Institute for Life Sciences have developed high-profile technological platforms in many areas : biomedical imaging, structural biology, metabolomics, High-Throughput screening, level 3 microbiological safety laboratory. (cea.fr)
Hybrid methods that combine computational biophysics with experimental structural biology have proved successful in describing protein conformation, namely their 3D shape. (europa.eu)
This is now becoming possible through the use of hybrid methods, which combine computational biophysics with experimental structural biology and overcome the limitations of either approach alone. (europa.eu)
We expect our results to be of great benefit to the broad structural biology community and to be instrumental in understanding brain physiology and designing treatments for a wide range of diseases. (europa.eu)
By the time he was in a PhD program at Berkeley, he'd dived deep into biochemistry and structural biology, studying how ribosomes and other higher-order assemblies take shape. (rockefeller.edu)
They also intrigued him because they seemed to merge a classical way to think about biology-as a system of genes and proteins-with the lesser understood concept of mechanobiology, "occupying an existential gray area between the microscopic chemical and the macroscopic physical worlds," Alushin says. (rockefeller.edu)
The volume of information included and the relevance to the field of structural biology are compared. (iucr.org)
Predicting and designing the structures of proteins with biologically useful accuracy has been a key challenge in computational structural biology and molecular engineering. (ucsf.edu)
Nanobodies have been employed effectively to trap transient conformations of medically relevant proteins for structural biology, facilitate non-invasive diagnostic imaging, imaging of dynamic processes in the cell, super resolution imaging of protein complexes , point of care diagnostic biosensors and as next generation cancer and SARS-CoV-2 therapies. (edu.au)
Section F, Structural biology communications. (lu.se)

Utilization of Fast Photochemical Oxidation of Proteins and Both Bottom-up and Top-down Mass Spectrometry for Structural Characterization of a Transcription Factor-dsDNA Complex. (cuni.cz)
Motif orientation matters: Structural characterization of TEAD1 recognition of genomic DNA. (cuni.cz)
2009. Characterization of sulfoxygenation and structural implications of human flavin-containing monooxygenase isoform 2 (FMO2.1) variants S195L and N413K. . (oregonstate.edu)
2017. Identification and structural characterization of the precursor conformation of the prion protein which directly initiates misfolding and oligomerization. . (ncbs.res.in)
3) Protein interactions and enzyme characterization. (manchester.ac.uk)

These isolates carried the mutations T2504A and C2534T in multiple 23S rRNA copies and 2 mutations leading to relevant amino acid substitutions in L3 protein. (cdc.gov)

Gurry T., Stu ltz CM. Intrinsically Disordered Proteins: Where Computation Meets Experiment. (mit.edu)
in Intrinsically Disordered Proteins . (mit.edu)
Stultz CM. Constructing Ensembles for Intrinsically Disordered Proteins. (mit.edu)
Huang A., Stultz CM. Modeling Intrinsically Disordered Proteins with Bayesian Statistics. (mit.edu)

Short coding motifs, which appear to lack secondary structure, include those that label proteins for delivery to particular parts of a cell, or mark them for phosphorylation. (wikipedia.org)
beta-Turns related by the characteristic threefold structural symmetry of this superfold exhibit different primary structures, and in some cases, different secondary structures. (rcsb.org)
Protein secondary and three-dimensional structure. (lu.se)
The amide I bands showed that the secondary structures of free and adsorbed protein molecules differ, and that the secondary structure of the adsorbed protein is influenced by the local geometry as well as by the surface chemistry of the nanopores. (rsc.org)
You will learn how proteins are produced, how they fold into their secondary and tertiary structures and how they interact with each other. (manchester.ac.uk)
Describe protein folds in terms of secondary structural elements and the restrictions on peptide structure imposed by the planarity of the peptide bond. (manchester.ac.uk)

Moreover, targeting protein functional motions bears a great potential to control and modulate proteins' activities and interactions in physio-pathological contexts. (biorxiv.org)
These techniques provide valuable insights on proteins' functioning and interactions with their environment. (biorxiv.org)
Over the past several years, we have engineered a range of proteins with new functions, including protein-protein interactions that are specific enough to control complex biological processes in mammalian cells (Kapp*, Liu* et al. (ucsf.edu)
The interactions of proteins with the surface of cylindrical nanopores are systematically investigated to elucidate how surface curvature and surface chemistry affect the conformation and activity of confined proteins in an aqueous, buffered environment. (rsc.org)

Biochimica et Biophysica Acta - Proteins and Proteomics , 2022, Roč. (cuni.cz)

Intramolecular Backbone···Backbone Hydrogen Bonds in Polypeptide Conformations. (ncbs.res.in)
Basic knowledge of protein structure: polypeptide conformation. (lu.se)
Despite its deleterious impact on fitness, protein aggregation is a generic property of polypeptide chains, indissociable from protein structure and function. (uab.cat)

Scholars@Duke publication: Biochemical and structural studies of tenascin/hexabrachion proteins. (duke.edu)
The technique promises insight into the intracellular behavior of disease-causing proteins and novel drug screening applications, allowing in-situ visualization of how proteins respond to biochemical stimuli. (analytica-world.com)
Activated receptors directly or indirectly regulate cellular biochemical processes (eg, ion conductance, protein phosphorylation, DNA transcription, enzymatic activity). (msdmanuals.com)

He is currently well known for developing approaches to predict macromolecular structures, having participated in many Critical Assessment of Techniques for Protein Structure Prediction (CASP) competitions, where he criticised molecular dynamics for inability to refine protein structures. (wikipedia.org)
He has also worked on simplified representations of protein structure for analysing folding and packing, as well as developing scoring systems for large-scale sequence-structure comparisons. (wikipedia.org)
Given a structural motif of interest, such as a functional site, MA searches a target protein structure for a match: the set of atoms with the greatest geometric and chemical similarity. (nih.gov)
Structure of the EGL-1/CED-9 complex revealed that EGL-1 adopts an extended alpha-helical conformation and induces substantial structural rearrangements in CED-9 upon binding. (nih.gov)
A molecular dynamic (MD) modeling approach was applied to evaluate the effect of external electric field on gliadin protein structure and surface properties. (mdpi.com)
that is a stereotypical element of the overall structure of the protein. (wikipedia.org)
Here we report the cryo-electron microscopy structure of a tubular HIV-1 capsid-protein assembly at 8 Å resolution and the three-dimensional structure of a native HIV-1 core by cryo-electron tomography. (nature.com)
Structure of the amino-terminal core domain of the HIV-1 capsid protein. (nature.com)
Crystal structure of dimeric HIV-1 capsid protein. (nature.com)
Structure of the HIV-1 full-length capsid protein in a conformationally trapped unassembled state induced by small-molecule binding. (nature.com)
We have brilliant colleagues who are in the forefront of their respective research and focused on an understanding of protein structure and dynamics, and how we might understand how life actually works by probing these things at an intimate level of detail. (nih.gov)
Our method guaranties preservation of the protein structure during the transition and allows to access conformations that are unreachable with classical normal mode analysis. (biorxiv.org)
This allows us to produce a wide range of motions, some of them previously inaccessible, and to preserve the structure of the protein during the motion. (biorxiv.org)
Nevertheless, experimental protein structure determination remains a time consuming and costly process. (biorxiv.org)
The cryo-electron microscopy structure reveals domain organization and structural details of the Nf1 exon 23a splicing 3 isoform 2 in a closed, self-inhibited, Zn-stabilized state and an open state. (nature.com)
Burger V., Arenas D., Stultz CM. A Structure-free Method for Quantifying Conformational Flexibility in proteins. (mit.edu)
Burger V., Nolasco D., Stultz CM., Expanding the Range of Protein Function at the Far end of the Order-Structure Continuum. (mit.edu)
Linder D., Gurry T., Stultz CM. Towards a Consensus in Protein Structure Nomenclature. (mit.edu)
Stultz CM. Protein Structure along the order-disorder continuum. (mit.edu)
Intrinsically disordered regions (IDRs) lacking a fixed three-dimensional protein structure are widespread and play a central role in cell regulation. (iucr.org)
We also aim at an understanding of the forces that underpin the structures of proteins, as well as a basic understanding of the methods used in structure-based drug design. (lu.se)
assimilate and critically evaluate the scientific literature dealing with protein structure and function, particularly in terms of the experimental methods. (lu.se)
Training in the relevant theoretical and experimental methods described for the study of protein structure and dynamics. (lu.se)
Includes protein crystallization, data collection at MAX IV, data processing, structure determination and modelling, as well as a simple exercise in ligand docking. (lu.se)
For instance, many proteins share the same native structure yet their cellular dynamics and function, in other words their energy landscapes, are different. (berkeley.edu)
This would fundamentally change our conventional thinking about protein structure-function relationship, no? (alzforum.org)
The reason why Peter Lansbury and others used the term 'natively unfolded' for a-synuclein and other proteins, especially peptides, is that they do not exist, to a significant degree, in the same kind of stable structure that larger native proteins like myoglobin do. (alzforum.org)
I do not think there is a contradiction to the conventional thinking of protein structure-function relation. (alzforum.org)
In this paper, we describe an extension of DeepView/Swiss-PdbViewer through which structural motifs may be defined and searched for in large protein structure databases, and we show that common structural motifs involved in stabilizing protein folds are present in evolutionarily and structurally unrelated proteins, also in deeply buried locations which are not obviously related to protein function. (biomedcentral.com)
The three-dimensional structure of proteins has been an extensively studied topic for several decades. (biomedcentral.com)
Subsequently, a variety of further patterns and regularities ( e.g. , [ 2 - 4 ]) in protein structures have been found, that have proven useful in the context of protein structure determination and quality assessment of determined structures. (biomedcentral.com)
Proteins are involved in virtually all biological processes and their structure is key to their function. (manchester.ac.uk)
The emphasis of the course is on practical applications, for example selecting the best technique to address a particular question on protein structure and function. (manchester.ac.uk)
However, this protein has regions that can vary in structure, meaning that P. falciparaum can generate many VAR2CSA variants. (elifesciences.org)
Researchers from Tokyo Metropolitan University have successfully determined the high-resolution three-dimensional structure of proteins inside living eukaryotic cells. (analytica-world.com)
Unique statistical analysis with the help of Bayesian inference is applied to calculate the accurate 3D structure of the proteins with unprecedented precision. (analytica-world.com)
Their internal structure is extremely complex and varied, with an intricate structural hierarchy and a vast range of biomacromolecules distributed around a cytoskeletal network. (analytica-world.com)
With this new data, the team were able to elucidate the 3D structure of three model proteins with unprecedentedly high resolution, with a precision of 0.5 Angstroms (0.05 nanometers) for the position of the protein's main chain atoms. (analytica-world.com)
In particular, they identified a significantly different conformation in a localized region of one of the proteins compared to its reference structure in dilute solution. (analytica-world.com)
UT Southwestern researchers have identified the structure of a key member of a family of proteins called nicotinic acetylcholine receptors in three different shapes. (phys.org)

Alushin studies how these little-understood physical dynamics act on the cell's cytoskeleton, an internal network of protein filaments that constantly reconfigures itself to help the cell move, change shape, or ferry molecules from one cell compartment to another. (rockefeller.edu)
To provide core knowledge of the form, function and role of protein molecules in biological processes and further enable an understanding of biological phenomena at the molecular level. (manchester.ac.uk)
Red blood cells infected with the malaria parasite Plasmodium falciparum display a protein, VAR2CSA, which can recognize and bind CSA molecules present on placental cells and in placental blood spaces. (elifesciences.org)
The problem with simply applying the same techniques to proteins in sf9 cells was the significantly lower concentration of target proteins and short lifetime of cells, making it difficult to collect high quality multi-dimensional NMR spectra for nuclear Overhauser effect spectroscopy (NOESY) which would give precise information about how different atoms are spaced inside individual molecules. (analytica-world.com)
They plan to continue studying a7 in various cell types and how it interacts with other molecules and proteins. (phys.org)

Our novel interdisciplinary pipeline will involve the use of efficient coarse-grained representations of proteins from computational biophysics coupled with sophisticated image processing tools including 3D reconstruction, classification, and dimensionality reduction. (europa.eu)
Gregory M. Alushin , head of Rockefeller's Laboratory of Structural Biophysics and Mechanobiology , doesn't think about his career in science as a predestined calling as much as the result of accumulated experiences. (rockefeller.edu)
Protein Folding and Neurodegeneration: Biophysics to the Rescue? (alzforum.org)

Noncoding" sequences are not translated into proteins, and nucleic acids with such motifs need not deviate from the typical shape (e.g. the "B-form" DNA double helix). (wikipedia.org)

Due to their large surface area and ability to interact with proteins and peptides, graphene oxides offer valuable physiochemical and biological features for biomedical applications and have been successfully employed for optimizing scaffold architectures for a wide range of organs, from the skin to cardiac tissue. (mdpi.com)

The ability to directly visualize proteins on nanostructures in situ holds great promise for assessing the conformation and thickness of the protein corona, which is key to understanding and predicting the properties of engineered nanomaterials in a biological environment. (lu.se)

This study represents a breakthrough in our understanding of the pathological conformation of tau," commented Markus Zweckstetter of the Max Planck Institute for Biophysical Chemistry in Göttingen, Germany. (alzforum.org)

The course aims to provide a deeper understanding of some of the most important experimental methods used to determine the three-dimensional structures of proteins, as a basis for understanding their biological functions. (lu.se)

There is considerable use of graphs and underlying theory in the description of properties of proteins and how these are studied. (manchester.ac.uk)
The course covers both the principles that determine the properties of proteins and the experimental methods that are used to study these properties in modern molecular protein science. (lu.se)

Proteins are dynamic entities that undergo many structural transitions and fluctuations, which are essential to their biological functions. (europa.eu)
Proteins undergo an incredible transformation from one-dimensional sequence information into complex three-dimensional shapes that carry out intricate cellular functions. (berkeley.edu)
Another key result is the critical role of rare, transient, high-energy conformations in biological function: proteins spontaneously undergo transitions between multiple structural states that are either active or inactive. (lu.se)

Two globular proteins , lysozyme and myoglobin , with different catalytic functions, were used as model proteins to analyze structural changes in proteins after adsorption on ordered mesoporous silica SBA-15 and propyl-functionalized SBA-15 (C 3 SBA-15) with carefully controlled pore size. (rsc.org)

The EU-funded LeukoTheranostics project designed a proof of concept treatment for IBD by doping lipid nanoparticles with extraordinarily specific proteins that target only the immune cells that are actively causing inflammation. (europa.eu)
To tackle this challenge, a team from Tokyo Metropolitan University led by Assistant Professor Teppei Ikeya and Professor Yutaka Ito applied nuclear magnetic resonance (NMR) spectroscopy measurements to specific proteins expressed inside sf9 cultured insect cells, a strain of cells originally derived from a type of moth larva widely used for protein production. (analytica-world.com)

Fisher C., Ullman O., Stultz CM., Comparative Studies of Disordered Proteins with Similar Sequences: Application to AB40 and AB42. (mit.edu)
Actual algorithms work on both protein sequences and structures, some of them accounting also for conformational fluctuations around the native state and the protein microenvironment. (uab.cat)

In addition to the native conformation, a protein sequence populates small fluctuations around the native state, partially unfolded forms and even the globally unfolded conformation. (berkeley.edu)

We have made methodological advances that address one of the main bottlenecks: sampling the vast number of conformations accessible to proteins. (ucsf.edu)

Large macromolecules, including proteins and their complexes, very often adopt multiple conformations. (biorxiv.org)
Large macromolecules, including proteins and their complexes, are intrinsically flexible, and this flexibility is often linked with their function. (biorxiv.org)

A new study by researchers at Mahidol University in Bangkok, Thailand, reports a COVID-19 vaccine candidate called HexaPro in producing neutralizing antibodies against the SARS-CoV-2 spike protein in an animal model. (news-medical.net)
Our data show that the stable knockdown of HIPK2 led to wtp53 misfolding, as detected by p53 immunoprecipitation with conformation-specific antibodies, and that p53 protein misfolding impaired p53 DNA binding and transcription of target genes. (aacrjournals.org)

Today, recognition and classification of sequence motifs and protein folds is a mature field, thanks to the availability of numerous comprehensive and easy to use software packages and web-based services. (biomedcentral.com)

Allosteric Communication in the Multifunctional and Redox NQO1 Protein Studied by Cavity-Making Mutations. (cuni.cz)
The autosomal dominant monogenetic disease neurofibromatosis type 1 (NF1) affects approximately one in 3,000 individuals and is caused by mutations in the NF1 tumour suppressor gene, leading to dysfunction in the protein neurofibromin (Nf1) 1 , 2 . (nature.com)
Linezolid resistance in S. epidermidis has been attributed to specific 23S rRNA mutations (G2576U, G2447U, U2504A, C2534U, and G2631U) ( 5 , 6 ), cfr gene ( 7 ), or mutations in ribosomal proteins L3, L4, and L22 ( 7 ). (cdc.gov)
In 8 LRSE isolates representing all PFGE types, genes encoding the L3, L4, and L22 ribosomal proteins that factor in ribosome assembly were sequenced to identify mutations conferring linezolid resistance ( 6 ). (cdc.gov)

He shifted his focus from microtubules to actin filaments, another key part of the cytoskeleton made up of long polymers of proteins linked end-to-end. (rockefeller.edu)

This study demonstrated that it is necessary to gain insight into protein dynamics under external electric field stress, in order to develop the novel food processing techniques that can be potentially used to reduce or eradicate food allergens. (mdpi.com)
Funded by the Marie Skłodowska-Curie Actions programme, the EnLaCES project will present a new hybrid methodology that leverages recent innovations in cryogenic electron microscopy to examine the continuous dynamics and energy landscapes of large, multi-domain proteins. (europa.eu)
In this proposal, we present a new hybrid methodology that leverages recent innovations in cryo-electron microscopy image analysis to examine continuous dynamics and free energy landscapes of large, multi-domain proteins, which are not achievable with existing methods. (europa.eu)
I am interested in how protein dynamics contribute to biological function. (lu.se)
In my research group we primarily use nuclear magnetic resonance (NMR) spectroscopy to study protein dynamics over a wide range of time scales, from picoseconds to seconds. (lu.se)
We address specific questions regarding the role of protein dynamics in various types of function and dysfunction, for example: ligand binding and allostery, enzyme catalysis, and protein misfolding and aggregation. (lu.se)

Back then, electron microscopy methods to study structures were in their infancy, so one of the things we had to do was to take pictures of proteins using photographic film," he says. (rockefeller.edu)
Our use of different hydrogen exchange methods to study three different types of intermediates of E. coli ribonuclease H (the acid molten globule, the higher energy conformations of the native state and a kinetic folding intermediate) has been particularly powerful. (berkeley.edu)
I. Develop computational methods for modeling & design of proteins, in the program Rosetta ( www.rosettacommons.org ). (ucsf.edu)
Thus, they combined a sparse sampling -based rapid NMR measurement scheme with state-of-the-art computational methods employing statistical techniques like Bayesian inference , methods tailored to elucidate protein structures efficiently based on a limited amount of structural information from in-cell NMR spectra with inherently low-sensitivity. (analytica-world.com)

Walker S., Ullman O., Stultz CM. Using Intramolecular Disulfide Bonds in Tau Protein to Deduce Structural Features of Aggregation-resistant Conformations. (mit.edu)
See also David Thirumalai's Emerging Ideas on the Molecular Basis of Protein and Peptide Aggregation ' (.pdf). (alzforum.org)
Protein aggregation is behind the onset of neurodegenerative disorders and one of the serious obstacles in the production of protein-based therapeutics. (uab.cat)
The development of computational tools opened a new avenue to rationalize this phenomenon, enabling prediction of the aggregation propensity of individual proteins as well as proteome-wide analysis. (uab.cat)
These studies spotted aggregation as a major force driving protein evolution. (uab.cat)
This toolbox allows to delineate conformation-specific routines to assist in the identification of aggregation-prone regions and to guide the optimization of more soluble and stable biotherapeutics. (uab.cat)
Here we review how the advent of predictive tools has change the way we think and address protein aggregation. (uab.cat)

We present a structural model for the assembly of the observed fragments into the elaborate native hexabrachion. (duke.edu)

For example, LAB exhibit peptidolytic and proteolytic activity that could break down the proteins causing wine haze, potentially reducing the need for bentonite addition. (frontiersin.org)

Our results show that the hierarchy of functional importance within structural motifs speeds up the search within targets, and points to a new method to score their statistical significance. (nih.gov)

Other projects in the lab focus on the mechanism of protein misfolding, the structural and energetic mechanism of signaling, computational identification of folding modules, and the role of ligand binding. (berkeley.edu)

These types of transitions can mediate allosteric signaling within proteins via distinct pathways that we have detected using NMR spectroscopy. (lu.se)

One example is a study describing the control of precise shape transitions of a large protein assembly with optical inputs, where we successfully exchanged the 'engine' of a protein-based ATP-driven molecular machine to be powered by light (Hoersch et al. (ucsf.edu)

Liquid phase ATR-FTIR spectroscopy was used to study the amide I and II bands of the adsorbed proteins . (rsc.org)
Available online 25 January 2010 peaks have been linked to a dipolar flip-flop polarization transfer from 1H nuclei to rapidly relaxing amide 14N nuclei in rotationally immobilized proteins. (lu.se)

Our high-resolution single particle cryo-electron microscopy (EM) structures reveal the domain organization and structural details of the full-length Nf1-23a isoform 2 dimer (Fig. 1a-c , Extended Data Figs. 1 - 3 ). (nature.com)

Recognition of structural motifs, by comparison, is less well developed and much less frequently used, possibly due to a lack of easily accessible and easy to use software. (biomedcentral.com)
The possibility to define custom motifs and search for their occurrence in other proteins permits the identification of recurrent arrangements of residues that could have structural implications. (biomedcentral.com)
Our purpose in this paper is to present the mechanisms and facilities whereby structural motifs can be defined and searched for using the freely available and well established modelling tool Swiss-PdbViewer [ 26 ], and to present illustrative examples of the types of information that can be obtained by doing so. (biomedcentral.com)
In particular, the facilities for defining and searching for structural motifs now available in Swiss-PdbViewer include an interactive visual interface for defining structural motifs, and a machinery that is able to quickly search very large collections of structures for such motifs. (biomedcentral.com)
The main reason for our interest in general ( i.e. , sequentially non-contiguous) structural motifs, is the crucial role played by side-chains in the correct packing of proteins. (biomedcentral.com)

Nicotinic acetylcholine receptors are members of a broader super-family of proteins called Cys-loop receptors that function as ion channels on cell surfaces and are found in the membranes of many cell types. (phys.org)

Our predictions generate hypotheses on protein conformations controlling biological processes - such as protein recognition, signal transduction, and enzyme active site gating - and are laying the foundation for our work reengineering and "reshaping" protein interfaces and active sites for new functions. (ucsf.edu)

The comparison of structural subsites in proteins is increasingly relevant to the prediction of their biological function. (nih.gov)
MA is extremely efficient because it exploits the fact that the amino acids in a structural motif are not equally important to function. (nih.gov)
This structural heterogeneity is not occasional and is frequently linked with specific biological function. (biorxiv.org)
As a GTPase-activating protein, a key function of Nf1 is repression of the Ras oncogene signalling cascade. (nature.com)
Homeodomain-interacting protein kinase-2 (HIPK2) is a positive regulator of p53 oncosuppressor function. (aacrjournals.org)
Such non-native states on the energy landscape are thought to play a determining function in many cellular processes such as translocation, protein synthesis, degradation, signaling and allostery. (berkeley.edu)
Our studies on the mechanism of folding include investigating the role of topological complexity using permuted proteins, the nature and function of folding intermediates, and studies on the role of mechanical unfolding using single molecule measurements. (berkeley.edu)
Analyze data relating to protein function and demonstrate an understanding of associated variables (resolution, Kd, Km & Vmax). (manchester.ac.uk)
The general aim of the course is to enable students to acquire an advanced understanding of proteins with an emphasis on their three-dimensional structures, the connection of structures to biological function and how these structures are produced. (lu.se)

The systematic description of the variety of shapes a protein adopts under particular environmental conditions, upon post-translational modifications and/or partner binding still remains out of reach. (biorxiv.org)

The lifetime of the drug-receptor complex is affected by dynamic processes (conformation changes) that control the rate of drug association and dissociation from the target. (msdmanuals.com)

Describe the features of proteins which lead to successful folding, post-translational modification and degradation. (manchester.ac.uk)

They combined "in-cell" nuclear magnetic resonance (NMR) spectroscopy, a bioreactor system and cutting-edge computational algorithms to determine protein structures in crowded intracellular environments for the first time. (analytica-world.com)

Target proteins are expressed inside sf9 cells, then measured using "in-cell" NMR spectroscopy. (analytica-world.com)

Anatomy12

Organisms38

Diseases1

Chemicals and Drugs83

Analytical, Diagnostic and Therapeutic Techniques and Equipment49

Phenomena and Processes75

Disciplines and Occupations5

Information Science10

Health Care2

Recombinant Fusion P1

Crystallography3

Stability9

Biology12

Characterization5

Amino Acid1

Intrinsically Disordered Proteins4

Secondary6

Interactions4

Proteomics1

Polypeptide3

Biochemical3

Structure40

Molecules5

Biophysics3

Nucleic acids1

Peptides1

Nanostructures1

Chemistry1

Structures of proteins1

Properties of proteins2

Undergo3

Globular1

Specific proteins2

Sequences2

Fluctuations around the native state1

Advances1

Macromolecules2

Antibodies2

Classification1

Mutations4

Polymers1

Dynamics6

Methods4

Aggregation7

Fragments1

Proteolytic1

Functional1

Ligand1

Allosteric1

Transitions1

Amide2

Cryo-electron m1

Motifs5

Receptors1

Interfaces1

Function9

Adopts1

Dynamic processes1

Degradation1

Algorithms1

Spectroscopy1