Structural protein engineering. Medical search. Frequent questions

Milan Mrksich , the Henry Wade Rogers Professor of Biomedical Engineering, Chemistry, and Cell and Molecular Biology at Northwestern's McCormick School of Engineering, is a co-author on the paper. (northwestern.edu)
My research focuses on understanding the intricate molecular mechanisms that determine the vast repertoire of peptide antigens displayed by the proteins of the Major Histocompatibility Complex for immune surveillance by T cells and Natural Killer cells. (upenn.edu)
Our detailed biophysical characterization of the molecular chaperone TAPBPR provided us with a high-resolution view of peptide exchange on MHC-I proteins and led to the development a high-throughput methodology for generating barcoded MHC tetramer libraries encompassing hundreds of unique antigen specificities. (upenn.edu)
probes, molecular reporters and fluorescent indicators for structural or functional imaging or microscopy/nanoscopy. (nih.gov)
First, utilizing computational structural biology techniques, I investigate the molecular mechanism that enables engineered adeno-associated viral (AAV) capsids to cross the blood-brain barrier (BBB). (caltech.edu)
We seek to understand the molecular basis of this fine-tuned self-foreign discrimination system by applying interdisciplinary approaches such as, antigen-discovery platforms, protein engineering techniques and structural biology tools. (mskcc.org)
I co-convene BIOL2162/6162 Molecular Gene Technology with Dr Tony Millar, and teach modules in BIOL3106 (Plant and Animal Bacterial Pathogenesis) and BIOL3107 (Structure and Function of Protein Kinases in Plant Immunity). (edu.au)
Unique spatial arrangement of polypeptide chains yields 3D molecular structures, which define protein function and interactions with other biomolecules. (kdnuggets.com)
studies at University of Oxford, where I learned the modern aspects of (b-lactam antibiotics) biosynthesis, molecular biology and enzymology (structural and mechanistic). (bradford.ac.uk)
PROFESSIONAL EXPERIENCE: Postdoc (2003-2006) at the Jean-Pierre Ebel Structural Biology Institute (IBS) of the Scientific Campus of Grenoble (France), which includes the European Synchrotron Radiation Facility (ESRF), the Institut Laue-Langevin for neutron science (ILL), and the European Molecular Biology Lab (EMBL). (iit.it)
The research in my lab is at the interface of chemistry and biology to investigate the molecular basis of diseases and discover novel therapeutic/technological approaches (e.g., ligands, biodrugs, nanodiscs, targeted nanovectors, lipid-nanoparticles for nucleic acid delivery, engineered viral cages and antibodies). (iit.it)
This work resulted in a faculty position in the Department of Biochemistry, Molecular Biology and cell Biology, Northestern Univerisy in Chicago.During this time he worked on the structure and function of the enzyme Dienelactone Hydrolase and as part of this study he was involved in the identification of a new protein fold - the alpha / beta hydroalse fold. (edu.au)
The Center for Molecular Protein Science (CMPS) brings together scientists active within the fields of biochemistry, molecular biophysics, structural biology, and physical and theoretical chemistry. (lu.se)
We want to understand the molecular mechanisms of biological processes by exploring the structure, dynamics, interactions and function of proteins. (lu.se)
Mauno Vihinen is well-known for his experience and interest in investigating variations and their effects whether they emerge at molecular levels (DNA, RNA protein), in structural context or in the cellular networks and pathways. (lu.se)
The experiment shows that the setup, based on JUNGFRAU detector and Jungfraujoch data-acquisition system, can provide a molecular moving picture of up to 500 microseconds in resolution of protein dynamics - providing ten times finer details than the previously available method. (lu.se)

DCAF15 has a distinct topology that embraces the RBM39(RRM2) domain largely via non-polar interactions, and indisulam binds between DCAF15 and RBM39(RRM2), coordinating additional interactions between the two proteins. (nature.com)
The class-I and class-II proteins of the Major Histocompatibility Complex play an essential role in immune surveillance, by displaying epitopic peptides derived from the processing of aberrant proteins on the cell surface, where they can be recognized through interactions with specialized receptors on T cells and Natural Killer cells. (upenn.edu)
It has been attributed to specific patterns in protein sequence , and it has an immediate consequence for protein stability , susceptibility to enzymatic digestion inside living cells, protein-protein interactions and in turn a decisive role in many debilitating human pathologies . (kdnuggets.com)
We engineer the membranes of microbes, i.e. bacteria and yeasts, regarding lipid and membrane protein composition, either to understand the complex interactions of these hydrophobic compounds or to apply the modified microorganisms for interesting goals of industrial biotechnology. (tugraz.at)
Our particular focus in the yeast lipid field are sterols, their biosynthesis and homeostasis, and their interactions with membrane proteins. (tugraz.at)
This reviews the pairing of next generation sequencing technologies with high throughput screens for studying and designing protein-protein interactions and enzymatic functions. (msu.edu)
Non-cellular assessment of nanoparticle durability, protein interactions, complement activation, and pro-oxidant activity is also considered. (cdc.gov)

Sugar structures allow these proteins to remain stable while enabling them to perform tasks, like attack a cancer cell or retrain the immune system. (northwestern.edu)
These mammalian cells naturally produce glycosylated proteins, but are slow-growing and can be difficult to engineer, limiting the number and diversity of glycosylation structures that can be built and tested. (northwestern.edu)
In just a few months, Kightlinger used the system to construct 37 pathways, creating 23 unique sugar structures, 18 of which have never been synthesized on proteins. (northwestern.edu)
This review critically focuses on opportunities to employ protein-graphene oxide structures either as nanocomposites or as biocomplexes and highlights the effects of carbonaceous nanostructures on protein conformation and structural stability for applications in tissue engineering and regenerative medicine. (mdpi.com)
It is accepted that complete understanding of protein functions and activity requires knowledge of structures and dynamics. (kdnuggets.com)
Following the doctrine of "Structure Determines Function", our research evolves around determining key protein structures that are mechanistically informative on biochemical processes. (luc.edu)
Putting together the obtained structures like snapshots, key knowledge on the biological functions of those critical proteins can be deduced. (luc.edu)
Structural biology - we use protein crystallography to determine the structures of interesting proteins - cell surface receptors such as the IL5 beta common receptor being one example. (edu.au)
Most of our structure work is aimed at looking at the structures of proteins that have been evolved to have enhanced activities. (edu.au)
During this time, Professor Ollis worked on the first structure of a DNA polymerase as well as the structures of a number of other proteins. (edu.au)
The major part of his production relates to variations ranging from protein engineering to effects and mechanisms of variations in protein structures, genes and diseases. (lu.se)

Jewett's lab has developed cell-free systems that create enzymes needed to create certain proteins, but up until now, these processes could not create glycosylated products without the need to reengineer living cells. (northwestern.edu)
Weston Kightlinger, a PhD student in the Jewett lab, developed a new approach to build, test, and assess sets of enzymes that can modularly build sugars for protein therapeutics. (northwestern.edu)
Moreover, NiRs are structurally homologous enzymes, performing the same reaction, but exhibiting a wide diversity of structural controls. (europa.eu)
Frequently, researchers are aiming at improving catalytic performance of protein enzymes, or adding completely new types of chemical activities to known proteins. (kdnuggets.com)
Challenging chemical transformations and forcing enzymes, via engineering, to catalyse these reactions, with exquisite regio- and stereoselectivity, for biocatalytic purposes, is one of the main themes of my research. (bradford.ac.uk)
Extending my research from engineering enzymes to redesign of biosynthetic pathways to produce unnatural products of pharmaceutical interest is another objective for my research. (bradford.ac.uk)
Rational and evolutionary enzyme engineering approaches allow us to make novel enzymes or enzyme families fit for biocatalytic applications, as isolated enzymes or in whole-cell applications. (tugraz.at)

3DSIG focuses on structural bioinformatics and computational biophysics. (iscb.org)
Computational studies and Bioinformatics is an interdisciplinary field that progresses and applies computational methods to explore biological data collections such as protein samples, cell populations and genetic sequences for newer predictions or find out new Biology. (wikicfp.com)

More and more, we realize the important link between PTFPs protein dynamics and PTFPs photophysical behavior, so that NMR , which can address the dynamical behavior of proteins with great detail, is also becoming a central tool for our investigations. (ibs.fr)
This includes gene, protein and metabolic networks, cellular architecture and intracellular dynamics, cell communication and motility, cell division and differentiation, tissue formation and organogenesis, tissue and organ functions, changes in population characteristics as a consequence of interaction of organisms with their physical environment, with individuals of their own species, and with organisms of other species. (nih.gov)
Moreover, I have intentionally left out a fundamentally important fact - mutations may significantly affect protein dynamics, and thus its function). (kdnuggets.com)
Protein structure, function and dynamics predictions through Machine Learning methodology are not an exception. (kdnuggets.com)
However, even with the relatively sparse (compared to a number of possible combinations of all protein amino acids in lengthy polypeptide chains) protein databases, Machine Learning can help to unravel complex, non-linear relationships between protein sequences and their structural variability and dynamics. (kdnuggets.com)
Thus predictions using complex numerical models that underlie Machine Learning methodology, can be further tweaked and refined by providing independent experimental proxies of protein structure and dynamics. (kdnuggets.com)
CONTACT-guided mutagenesis can exploit the structure-dynamics-function relationship in protein engineering and design. (rcsb.org)

Our lab's principal efforts focus on mechanistic, structural and functional studies of protein complexes, with emphasis on the development of an iterative experimental / computational approach. (upenn.edu)
As of July 2022, the unit has produced assays for more than 1,500 recombinant proteins, including multisubunit complexes, DNA- and RNA-modifying proteins, and membrane proteins. (bioprocessintl.com)
We use these engineered Cas9 activation complexes to investigate sgRNA targeting rules for effective transcriptional activation, demonstrate multiplexed activation of 10 genes simultaneously, and upregulate long intergenic non-coding RNA (lincRNA) transcripts. (cdc.gov)

These have enabled associated mechanistic details of protein and sugar biology to be elucidated and exploited for biotechnological applications. (wikipedia.org)
Dr. Liu's research group primarily employs X-ray crystallography in combination with mechanistic, biological and computational methods to study proteins involved in bacterial infections and other human diseases. (luc.edu)

The isolated antigens and their parent proteins for 'orphan' TCRs would then allow specific perturbation on each target to see how T cell responses are affected. (mskcc.org)
In particular, we developed 2 IA assays, in which the engineered antigens were used either as capture (F1 format) or detector (F2 format), resulting in slight difference in sensitivity and specificity. (cdc.gov)

We combine high-throughput enzyme discovery and evolution with structure-based rational engineering to elucidate the structural determinants of key enzymatic properties such as Substrate specificityEnzymatic efficiencyThermostability and pH tolerance Our core methods are X-ray crystallography, enzymology, and other classical protein chemistry techniques for biophysical characterization. (dtu.dk)
To gain an atomic-detail description of these processes and to characterize novel therapeutic targets, we integrate cutting-edge structural biology methods from Nuclear Magnetic Resonance Spectroscopy, complementary biophysical and biochemical techniques, computational modeling and functional immunoassays. (upenn.edu)
The biggest value of Machine Learning methods in prediction of biophysical properties of proteins is their ability to " equate " loosely related protein features to measurable experimental data. (kdnuggets.com)
In the future, Odessa aims to apply her expertise of structural biology and modern biophysical techniques to engineer novel function into proteins. (unc.edu)
Today, the unit provides a rapid and cost-effective platform for protein generation, screening, biophysical and biochemical analysis, structural determination, and "hit" confirmation. (bioprocessintl.com)
CMPS is a highly integrated center comprising the divisions of Biochemistry and Structural Biology (Faculty of Science) and Biophysical Chemistry (Faculty of Engineering, LTH). (lu.se)

Once the viruses and their gene cargoes infected liver cells, the infected cells expressed the gene and produced iRFP protein. (sciencedaily.com)
Programmable DNA binding proteins have emerged as an exciting platform for engineering synthetic transcription factors for modulating endogenous gene expression 5 - 11 . (cdc.gov)
[ 1 ] is a congenital hypomyelination disorder caused by changes affecting the proteolipid protein 1 gene (PLP1) located on Xq22.2. (medscape.com)
Although Pelizaeus-Merzbacher disease and X-linked spastic paraplegia type 2 are nosologically distinguished, they are at opposite ends of a clinical spectrum of X-linked diseases caused by mutations of the same gene, the proteolipid protein 1 ( PLP1 ) gene, and result in defective central nervous system (CNS) myelination (see the image below). (medscape.com)

Pichia pastoris , a methylotrophic yeast, is our favourite host, which we employ for expression and secretion of recombinant soluble proteins as well as for the biosynthesis of valuable metabolites, e.g. plant terpenoids, through heterologous expression of membrane proteins. (tugraz.at)

Upregulation of several target genes has improved recombinant protein titers and yields. (tugraz.at)

UDG activity is controlled by various cellular factors, including the uracil-DNA glycosylase inhibitors, which are DNA mimic proteins that prevent the DNA binding sites of UDGs from interacting with their DNA substrate. (tmu.edu.tw)
Glycosylation - the attachment of sugars to proteins - plays a critical role in both cellular function and in the development of therapeutics, like vaccines. (northwestern.edu)
The latter represents a conformationally modified form of a normal cellular PrP C , which is a normal host protein found on the surface of many cells, in particular neurons. (medscape.com)

Assay-grade proteins can be expressed using microbial, insect, or mammalian cell lines, and regardless of host, production activities typically can be performed in four to eight weeks. (bioprocessintl.com)
This new phytochrome-based fluorescent protein, dubbed iRFP, both absorbs and emits light in the near-infrared portion of the electromagnetic spectrum- the spectral region in which mammalian tissues are nearly transparent. (sciencedaily.com)

nanoBIOTECHNOLOGY , with applications that range from design and generation of bioactive 3D (nano-)biomaterials (e.g., nanodiscs, targeted nanovectors, lipid-nanoparticles for nucleic acid delivery, engineered viral cages and antibodies) to biodrugs and cascade biocatalysts in sustainable technology. (iit.it)
Highly divergent hypotheses have been put forward regarding the makeup of the prions, including that they consist of nucleic acid only or protein only, are lacking both protein and nucleic acid, or are a polysaccharide. (medscape.com)

Get high medical value with Access SARS-CoV-2 IgG assay as it detects antibodies against the virus spike protein that may be more likely to confer immunity. (beckmancoulter.com)
Studies have also shown that the S protein plays the most important roles in viral attachment, fusion and entry, and it serves as a target for development of antibodies, entry inhibitors and vaccines. (beckmancoulter.com)
The coronavirus spike protein mediates entry into host cells by attaching to a receptor on respiratory cells called angiotensin-converting enzyme 2, or ACE2, 11 the existence of antibodies against it may suggest neutralization of SARS-CoV-2 infection. (beckmancoulter.com)
The Access SARS-CoV-2 IgG assay is designed to detect antibodies to the RBD (receptor-binding domain) of the spike protein, which may be important for immunity. (beckmancoulter.com)
We engineered the ZIKV nonstructural protein 1 (NS1) for sensitive serologic detection with low cross reactivity against dengue and developed monoclonal antibodies specific for the ZIKV NS1 antigen. (cdc.gov)
These amyloid plaques are immunoreactive with antibodies to the prion protein and do not immunoreact with antibodies to other amyloidogenic proteins, such as the amyloid-beta (which is deposited in Alzheimer disease). (medscape.com)
a foreign substance, usually protein or carbohydrate substance (as a toxin or enzyme) capable of stimulating an immune response, usually the production of antibodies. (cdc.gov)

One of our central themes concerns photophysical studies of phototransformable fluorescent proteins (PTFPs). (ibs.fr)
In addition, photoblinking (the stochastic and transient loss of fluorescence) and photobleaching (the irreversible loss of fluorescence) are crucial photophysical properties that apply to all fluorescent proteins and that we investigate in great details, although they still hide a lot of mysteries. (ibs.fr)
Many groups worldwide study and develop fluorescent proteins to create ever more performing markers or sensors. (ibs.fr)
For the past 20 years, scientists have used a variety of colored fluorescent proteins, derived from jellyfish and corals, to visualize cells and their organelles and molecules. (sciencedaily.com)
The reason: hemoglobin in an animal's blood effectively absorbs the blue, green, red and other wavelengths used to stimulate standard fluorescent proteins along with any wavelengths emitted by the proteins when they do light up. (sciencedaily.com)
Our study found that iRFP was far superior to the other fluorescent proteins that reportedly help in visualizing the livers of live animals," said Grigory Filonov, Ph.D., a postdoctoral fellow in Dr. Verkhusha''''s laboratory at Einstein, and the first author of the Nature Biotechnology paper. (sciencedaily.com)
iRFP not only produced a far brighter image, with higher contrast than the other fluorescent proteins, but was also very stable over time. (sciencedaily.com)

Proteins are complex biomolecules made of 20 building blocks , amino acids, which are connected sequentially into long non-branching chains, commonly known as polypeptide chains. (kdnuggets.com)

Matching of structural motifs using hashing on residue labels and geometric filtering for protein function prediction. (uni-marburg.de)

Therefore, we study enzyme characteristics by classical protein analytics and devise novel enzyme assays. (tugraz.at)
Drug developers can leverage such assays for focused screening activities - e.g., to determine whether a chemical entity binds with a given protein. (bioprocessintl.com)

His group's research centres on the chemical understanding and exploitation of biomolecular function (Synthetic Biology, Chemical Biology and Chemical Medicine), with an emphasis on carbohydrates and proteins. (wikipedia.org)
His certificate of election reads: Professor Davis is noted for his chemical interrogation and manipulation of biological systems, particularly those that hinge on carbohydrates and proteins. (wikipedia.org)

Ciechanover, A. Intracellular protein degradation: from a vague Idea, through the lysosome and the ubiquitin-proteasome system, and onto human diseases and drug targeting (Nobel Lecture). (nature.com)

The ability to directly visualize proteins on nanostructures in situ holds great promise for assessing the conformation and thickness of the protein corona, which is key to understanding and predicting the properties of engineered nanomaterials in a biological environment. (lu.se)
To understand the potential toxicology of engineered nanomaterials it is fundamental to understand how they deposit in our respiratory system, and what the mechanisms of effects are. (lu.se)
We study the interaction between proteins in biological fluids as well as single proteins to nanomaterials of different kinds. (lu.se)

Bondeson, D. P. & Crew, C. M. Targeted protein degradation by small molecules. (nature.com)

We have uncovered several endogenous yeast proteins and mechanisms that render protein secretion or membrane protein expression more efficient and sustainable. (tugraz.at)

We are also interested using directed evolution to predict antibiotic resistance and have developed protocols to evolve proteins to be more soluble. (edu.au)

Cytoskeletal proteins as targets for organophosphorous compound and aliphatic hexacarbo n- induced neurotoxicity. (cdc.gov)

The researchers used this process to develop a protein vaccine candidate modified with a sugar structure that could trigger the immune system, as well as a therapeutic antibody fragment with a sugar that can stabilize proteins as they circulate in the body. (northwestern.edu)
My Ph.D. projects centered on using computational structural biology tools to develop protein engineering methods for targeted therapeutic delivery, emphasizing delivering molecules to the brain. (caltech.edu)
on-going studies aim to employ lactonase as a protein therapeutic to disrupt bacterial cell-to-cell communication, quorum-quenching. (luc.edu)

This is an exciting new method that accelerates the design and engineering of potential medicines and vaccines using glycosylation," said Michael Jewett , the Charles Deering McCormick Professor of Teaching Excellence, professor of chemical and biological engineering, and director of Northwestern's Center for Synthetic Biology , who led the research. (northwestern.edu)

We aim to study low concentrations of nanoparticles down to single particles in biologically relevant concentrations of proteins. (lu.se)

After his PhD, Davis spent two years as a postdoctoral research fellow in the laboratory of J. Bryan Jones [Wikidata] at the University of Toronto, exploring protein chemistry and biocatalysis. (wikipedia.org)
In structural chemistry and mineralogy. (royalsociety.org)
He was the first graduate student to solve a protein structure in Australia - this led to post-doctoral position in the laboraory of Professor Tom Steitz (Nobel Prize in Chemistry, 2008) of Yale University. (edu.au)
SciFinder-N provides access to the world's most comprehensive and authoritative source of references, substances and reactions in chemistry, chemical engineering, food, biomedicine and related sciences. (lu.se)
SpringerMaterials provides curated data and advanced functionalities to support research in materials science, physics, chemistry, engineering, and other related fields. (lu.se)

In the present work, accelerated methods for the comparison of protein binding sites as well as an extended procedure for the assessment of ligand poses in protein binding sites are presented. (uni-marburg.de)
Methods for the assessment of ligand poses in protein binding sites are also used in the early phase of drug development within docking programs. (uni-marburg.de)
How to Measure the Similarity Between Protein Ligand-binding Sites. (uni-marburg.de)
The Automatic Search for Ligand Binding Sites in Proteins of Known Three-dimensional Structure Using only Geometric Criteria. (uni-marburg.de)
Allosteric ligand binding or distal mutations can stabilize specific active-site conformations and consequently alter protein function. (rcsb.org)
PROTEIN BIOTECHNOLOGY , from design of engineered proteins to the structure of membrane protein targets for ligand discovery and biomolecular medicine. (iit.it)

Recently, in collaboration with the teams of J.B Sibarita and M. Sainlos ( IINS, Bordeaux ), we started an ANR-funded project aiming at further improving the photostability of PCFPs, by combining structural studies with high-content-screening single-molecule imaging approaches to achieve efficient semi-rational engineering. (ibs.fr)
We have applied multiple approaches to identify key genes that determine protein secretion rates in P. pastoris. (tugraz.at)
Primary RESEARCH TOOLS include approaches of structural biophysics, biotechnology and computation. (iit.it)

I show that APPRAISE is capable of distinguishing between receptor-dependent and receptor-independent adeno-associated viral vectors and ranking various engineered proteins, such as miniproteins binding to the SARS-CoV-2 spike and nanobodies binding to a G-protein-coupled receptor. (caltech.edu)
However, there are questions about which viral protein to use as an antigen for serology testing. (beckmancoulter.com)
Some diagnostic developers are not able to be definitive because this is a new virus, although based on the information we have, the viral protein is perceived as the obvious candidate. (beckmancoulter.com)
After the spike protein binds to the human cell receptor, the viral membrane fuses with the human cell membrane, allowing the genome of the virus to enter human cells and begin infection. (beckmancoulter.com)

NWs incubated with both laminin and HSA show protein coronas with a similar appearance to NWs incubated with laminin alone, indicating that the presence of HSA does not affect the laminin conformation on the NWs. (lu.se)
The infectious agent in the prion disease is composed mainly or entirely of an abnormal conformation of a host-encoded glycoprotein called the prion protein. (medscape.com)
The replication of prions involves the recruitment of the normally expressed prion protein, which has mainly an alpha-helical structure, into a disease-specific conformation that is rich in beta-sheet. (medscape.com)

The biofabrication process consists of engineering the DNA of yeast to produce collagen protein which is then fermented and eventually assembled into materials. (forbes.com)
Recently, we built an engineered Saccharomyces cerevisiae (yeast) which can produce both santalenes and santalols, with 35.7% Z - α -santalol and 17.8% Z - β -santalol which is very close to the standard of ISO 3518:2002 22 . (nature.com)

This study represents a paradigm of constructing biotechnological platforms of PEOs with desirable component ratios by the combination of metabolic and enzymatic engineering. (nature.com)

We currently focus on three protein targets involved in difference aspects of virulence expression in pathogenic bacteria. (luc.edu)

Research in the Davis laboratory has been funded by the Engineering and Physical Sciences Research Council, the Biotechnology and Biological Sciences Research Council, the Medical Research Council, UCB-Celltech, AstraZeneca, the European Union, GlaxoSmithKline, Cancer Research UK, the Wellcome Trust and the Royal Society. (wikipedia.org)
Protein biotechnology is to a large extent hampered by scale and complexity of mutational analysis . (kdnuggets.com)

Moreover, binding site comparisons are used as an idea generator for bioisosteric replacements of individual functional groups of the newly developed drug and to unravel the function of hitherto orphan proteins. (uni-marburg.de)

Through collaborative efforts, we are also interested in elucidating the mechanism of allosteric regulators and rational drug design against protein targets in humans. (luc.edu)

This extensive imaging methodology simultaneously reveals structural, chemical and morphological details of individual nanowires and the adsorbed proteins. (lu.se)

Due to their large surface area and ability to interact with proteins and peptides, graphene oxides offer valuable physiochemical and biological features for biomedical applications and have been successfully employed for optimizing scaffold architectures for a wide range of organs, from the skin to cardiac tissue. (mdpi.com)

Team leader (2010-2015) of the group of Structural Biophysics at the Dept of Drug Discovery and Development of the Istituto Italiano di Tecnologia (IIT), Genoa (Italy). (iit.it)

Biochimica et Biophysica Acta: Proteins and Proteomics 2009, 1794 (7), 995-1000. (ncl.ac.uk)
Later, I moved to NNF Center for Protein Research in Copenhagen, where I was working with application of machine learning on LC-MS/MS proteomics data and PPI networks. (lu.se)

The current focus is copper-containing proteins and we use a multi-disciplinary approach to study the relationship between their structure and function. (ncl.ac.uk)
RNA Biology: Tertiary Structure and RNA-Protein. (hhmi.org)
Bioenergetics: Protein Structure, Kinetics, and. (hhmi.org)
The NiR system presents an ensemble of favourable characteristics: It is well characterised from structural, biological and kinetical points of view: the structure of oxidised cd1 NiR from Thiosphaera pantotropha (Tp) has already been solved at 1.5A resolution, and that of Pseudomonas aeruginosa (Pa) is likely to be finished soon. (europa.eu)
https://www.livescience.com/coronavirus-spike-protein-structure.html. (beckmancoulter.com)
Although the very basic forces that govern protein 3D structure formation are known and understood, the exact nature of polypeptide folding remains elusive and has been studied extensively for the past 50 years. (kdnuggets.com)
We describe structure-guided engineering of a CRISPR-Cas9 complex to mediate efficient transcriptional activation at endogenous genomic loci. (cdc.gov)
In order to improve and expand applications of Cas9, we recently undertook crystallographic studies to elucidate the atomic structure of the Cas9-sgRNA-target DNA tertiary complex 14 , thus enabling rational engineering of Cas9 and sgRNA. (cdc.gov)

Enzyme Engineering and Structural Biology We develop enzyme biocatalysts for the industrial production of natural products used as e.g. aromas, fragrances, dyes, and pharmaceuticals. (dtu.dk)
In the case of SARS-CoV-2, the spike proteins bind angiotensin-converting enzyme 2 (ACE2 receptor) on human cells with high affinity. (beckmancoulter.com)

abstract = "Uracil-DNA glycosylases (UDGs) are highly conserved proteins that can be found in a wide range of organisms, and are involved in the DNA repair and host defense systems. (tmu.edu.tw)

Therefore, we have executed a rational surface mutagenesis strategy that has yielded crystals of this 2300-amino acid multidomain protein, diffracting to 2A or better. (rcsb.org)
and postdoctoral studies time, I have developed myself into a rational-design protein engineer. (bradford.ac.uk)

We are also developing methods to identify ligands for non-druggable protein surfaces. (stanford.edu)
The structural and spectral properties of these new macrocyclic ligands were thoroughly investigated, revealing promising coordination properties towards f-elements (Am, Eu). (bvsalud.org)

The researchers targeted their fluorescent protein to the liver -- an organ particularly difficult to visualize because of its high blood content. (sciencedaily.com)

The structural basis of immunology. (iscb.org)

His current research interests include protein engineering (direcetd evolution) and structural biology (protein crystallography). (edu.au)

Concurrent movements of a loop adjacent to the active site demonstrate how this protein can effect large changes in the size and shape of the substrate binding pocket to accommodate a diverse range of substrates. (acs.org)
Scaffolds play an important role in tissue engineering as a substrate that can mimic the native extracellular matrix and the properties of scaffolds have been shown to affect the cell behavior such as the cell attachment, proliferation and differentiation. (wjgnet.com)

Dr. Vladislav V. Yakovlev is a Fellow of the Optical Society of America, the American Institute of Medical and Biological Engineering and the International Society for Optics and Photonics. (tamu.edu)

Jag är anknuten till Integrated Structural Biology och UCMR. (umu.se)

This includes selective binding of proteins, structural change after binding and the functional implications. (lu.se)

Studies with RBM39 point mutants and indisulam analogs validated the structural model and defined the RBM39 α-helical degron motif. (nature.com)
Binding sites of other proteins which are similar to the binding site of the target protein can offer hints for possible side effects of a new drug prior to clinical studies. (uni-marburg.de)
Currently, Odessa studies computational protein engineering. (unc.edu)

Computational design of closely related proteins that adopt two well-defined but structurally divergent folds. (upenn.edu)
De novo design of a homo-trimeric amantadine-binding protein. (upenn.edu)
Since the flagellar filament is external to the cell membrane, this poses an engineering problem: namely, how can we design the flagellum such that a large number of proteins assemble outside the cell? (evcforum.net)
Protein Engineering Design and Selection 2007 Mar;20(3):133-41. (lu.se)

The field of tissue engineering is constantly evolving as it aims to develop bioengineered and functional tissues and organs for repair or replacement. (mdpi.com)
Thus, we are engaged in a systematic study of secondary metabolism GT structural and functional determinants, to provide the framework for efficient secondary metabolism GT discovery and engineering. (dtu.dk)

An order-to-disorder structural switch activates the FoxM1 transcription factor. (upenn.edu)
It is now clear that Armadillo and beta-catenin bind directly to members of the T-cell factor/lymphoid enhancer factor subfamily of HMG box DNA-binding proteins, forming bipartite transcription factors that regulate Wingless/Wnt responsive genes in both Drosophila and vertebrates. (embl.de)
Among the established custom DNA binding domains, Cas9 is most easily scaled to facilitate genome-scale perturbations 3 , 4 due to its simplicity of programming relative to zinc finger proteins and transcription activator-like effectors (TALEs). (cdc.gov)
Cas9 nuclease can be converted into an RNA-guided DNA binding protein (dCas9) via inactivation of its two catalytic domains 12 , 13 and then fused to transcription activation domains. (cdc.gov)

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