Structural k562 cells. Medical search. Frequent questions

VDACs are generally involved in the regulation of cell metabolism, mitochondrial apoptosis, and spermatogenesis. (wikipedia.org)
VDAC2 in particular has demonstrated a protective effect in cells undergoing mitochondrial apoptosis, and may even confer protection during aging. (wikipedia.org)
Apoptosis has proven to be tightly interwoven with other essential cell pathways. (wikipedia.org)
In later stages of apoptosis the entire cell becomes fragmented, forming a number of plasma membrane-bounded apoptotic bodies which contain nuclear and or cytoplasmic elements. (wikipedia.org)
Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) binds to death receptors and induces apoptosis in various cancer cell lines while sparing normal cells. (oncotarget.com)
ADI downregulates survivin, upregulates DR5 receptor and sensitizes cancer cells to TRAIL induced apoptosis. (oncotarget.com)
Many cancer cells are intrinsically resistant to TRAIL-induced apoptosis. (oncotarget.com)
Apoptosis induction potential of each compound determined by mitochondrial membrane potential analysis (Rho123), cleaved caspase-3 expression analysis by flow cytometry and immunofluorescent staining and cell morphology analysis by giemza, hematoxylin and eosin and Papanicolaou protocols. (ijpsonline.com)
In the cell morphology analyzes, chromatin condensation and marginalization, changes in the cell membrane, ghost cells and apoptotic bodies were observed as evidence of apoptosis formation. (ijpsonline.com)
HeLa, A549, and K562), both in vitro and in vivo , with the induction of apoptosis under 808 nm diode laser irradiation. (oncotarget.com)
Inhibition of DNA-dependent protein kinase catalytic subunit by small molecule inhibitor NU7026 sensitizes human leukemic K562 cells to benzene metabolite-induced apoptosis. (cdc.gov)
Numerous studies currently suggest that quadruplex formation in this overhang alters the structure and function of telomeres, inducing a damage response and rapid apoptosis in particular in cancer cells [ 14 - 24 ]. (hindawi.com)
however, the study of ultrafine Pt NCs is at the beginning stage and there are still plenty of challenges like synthesis of near-infrared (NIR) fluorescent Pt NCs, the explicit signal pathway of cell apoptosis, and attempt in diverse biological applications that need to be urgently tackled in future. (hindawi.com)
After treatment with β-iminophosphonate (E,Z)(3-Methyl-2-(pyridin2-ylimino)butyl)diphenylphosphine oxide (4e) proliferation/viability of cancer cells was strongly inhibited and in 65.61% of the cells signs of early apoptosis were observed. (japsonline.com)
The cell apoptosis was detected using flow cytometry. (lookformedical.com)

This protocol tracks engaged transcription complexes across functional genomic regions demonstrated in human K562 erythroleukemia cells. (scilifelab.se)
In K562 erythroleukemia cells, repeated stress refined basal and heat-induced transcription over mitotic division and decelerated termination-coupled pre-mRNA processing. (scilifelab.se)

Cell-based and in vitro assays show that Retro-2 blocks delivery of newly-synthesized TA-proteins to the ER-targeting factor ASNA1 (TRC40). (elifesciences.org)
Treatment with VIP or PACAP prior to in vitro LC Ag presentation to CD4+ T cells enhanced IL-17A, IL-6, and IL-4 production, decreased interferon (IFN)-γ and interleukin (IL)-22 release, and increased RORγt and Gata3 mRNA expression while decreasing T-bet expression. (syksignaling.com)
Intradermal administration of VIP or PACAP prior to application of a contact sensitizer at the injection site, followed by harvesting of draining lymph node CD4+ T cells EPZ015666 in vitro and stimulation with anti-CD3/anti-CD28 mAbs, enhanced IL-17A and IL-4 production but reduced production of IL-22 and IFN-γ. (syksignaling.com)
Bosutinib is a potent inhibitor of CML cell proliferation in vitro and has demonstrated promising activity in CML patients resistant or intolerant to IM as well as in newly diagnosed patients with chronic phase CML (CML-CP). (mdm2-receptor.com)

resulted in variations of IC50 values obtained in HeLa cells. (documents.pub)
complex 2 induced a sub-G1 peak of the cell cycle, in the HeLa cell line. (documents.pub)
Although studied in model human cell lines such as HeLa, very little is known about the role of the CSN in haemopoietic cells. (biomedcentral.com)
IP: HeLa cells Flow Cyt (intra): HEK293 cells, A-431 cells. (abcam.com)

Platelet-derived growth factor (PDGF) is involved in megakaryocytopoiesis and is secreted into the culture medium during megakaryocytic differentiation of human leukemic cells. (edu.hk)
Holyoake, T.L. A comparison of normal and leukemic stem cell biology in chronic myeloid leukemia. (eurekaselect.com)

In neutrophils and myeloid K562 cells ligand ICAM-1 or activating Ab binding promoted switchblade-like extension of the Mac-1 extracellular domain name and separation of the αM and β2 subunit cytoplasmic tails two structural hallmarks of integrin activation. (researchdataservice.com)
Greater than 95% knockdown of the non-catalytic subunit CSN2 and the deneddylating subunit CSN5 of the CSN was achieved in the human myeloid progenitor cell line K562. (biomedcentral.com)

These structural changes ultimately result in extension of the headpiece away from the cell surface in a switchblade-like motion and separation of the cytoplasmic tails of the α and β integrin subunits (5 12 Structural and functional studies suggest that integrins exist in a dynamic equilibrium between three different affinity says: low intermediate and high (5). (researchdataservice.com)
CBP-HSF2 structural and functional interplay in Rubinstein-Taybi neurodevelopmental disorder. (scilifelab.se)
We characterize the structural and functional interaction between CBP/EP300 and heat-shock factor 2 (HSF2), a tuner of brain cortical development and major player in prenatal stress responses in the neocortex: CBP/EP300 acetylates HSF2, leading to the stabilization of the HSF2 protein. (scilifelab.se)
The fusion protein benefits from structural and functional synergies between its two components and has an extended half-life in vivo. (oncotarget.com)
Scaffold/matrix attachment regions (S/MARs) are DNA elements that serve to compartmentalize the chromatin into structural and functional domains. (researchgate.net)
DNA replication allowing time for DNA repair.30,31 p21Cip1 interaction with PCNA results in the inhibition of PCNA and thereby causes G1 and G2 block in T cells.14,32 There was some association of p21Cip1 with PCNA in stimulated control Th1 cells, but the functional significance of this low-level interaction was not determined. (syksignaling.com)
The structural and functional integrity of this overhang is based on its association with a constellation of specific proteins, some of them belonging to the shelterin complex [ 10 ]. (hindawi.com)
The Golby Lab is a translational multidisciplinary group of investigators focusing on functional brain mapping using both structural and functional imaging techniques to guide neurosurgical planning and intra-operative decision making. (dana-farber.org)
We use multiple functional brain mapping techniques (fMRI, diffusion tensor imaging, and intracranial EEG) and structural and molecular imaging techniques to better define individual functional anatomy in patients with neurosurgical diseases, cross-validate information acquired through different techniques, and gain a better understanding of the relationship between these different brain signals. (dana-farber.org)
γδ T cells express unique T cell receptor (TCR) γ and TCR δ chains, with structural and functional heterogeneity. (explorationpub.com)
At present, the phenotypic heterogeneity and functional variation of human γδ T cells are increasing. (explorationpub.com)

DAB2 induction and megakaryocytic differentiation was abrogated when cells were co-treated with the PDGF receptor inhibitor STI571 or when the conditioned medium was derived from TPA-plus STI571-treated cells. (edu.hk)
This is supported by pharmacological targeting where the first-generation pan-BET bromodomain inhibitor JQ1(+) displays anti-inflammatory effects and inhibit tumor cell eradication, while the novel bivalent BET bromodomain inhibitor AZD5153, which shows differential activity towards BET family members, does not. (frontiersin.org)

The low affinity state is usually characterized by a compact structure in which the extracellular domain name is usually bent over and the integrin headpiece is usually in close proximity to the cell membrane with the cytoplasmic tails of the α and β subunits closely apposed (13). (researchdataservice.com)
Adjustments in manifestation of activin/inhibin subunits in Rps19-lacking zebrafish and RPS19-lacking human main cells and cell lines directed to activin upregulation. (acancerjourney.info)
We conclude that CSN2 is required for CSN integrity and the stability of individual CSN subunits, and postulate that CSN2 loss results in a phenotype distinct from that of cells lacking CSN5 possibly as a consequence of altered CSN5 activity within a resultant CSN subcomplex. (biomedcentral.com)

Chromatin looping is important for gene regulation, and studies of 3D chromatin structure across species and cell types have improved our understanding of the principles governing chromatin looping. (nature.com)
While many CTCF binding sites fall within transposable elements (TEs), their contribution to 3D chromatin structural evolution is unknown. (nature.com)
Here, we measured nascent transcription and chromatin accessibility in unconditioned cells and in the daughters of stress-exposed cells. (scilifelab.se)
Recent efforts have shown that structural variations (SVs) can disrupt three-dimensional genome organization and induce enhancer hijacking, yet no computational tools exist to identify such events from chromatin interaction data. (nature.com)

Intracellular signals associated with or triggered by integrin ligation can control cell survival differentiation proliferation and migration. (researchdataservice.com)
The regulated expression and degradation of proteins are critical to all aspects of cell development and proliferation. (biomedcentral.com)

Fingolimod is a sphingosine 1-phosphate (S1P) antagonist with IC50 of 0.033 nM in K562 and NK cells. (csnpharm.cn)
Interestingly, the half maximal inhibitory concentration (IC50) of BLMs in different cancer cell lines varies from nM to μM ranges. (lookformedical.com)

Loss of either CSN subunit dramatically reduced cell growth but resulted in distinct patterns of cell death. (biomedcentral.com)
Structural modeling suggested that six lysines per VP2 subunit are presumably addressable for bioconjugation on the CPV capsid exterior. (biomedcentral.com)

2Department of Cell and Molecular Biology, University of Technology Sydney, Broadway, NSW, 2007, Australia JJN3 MMk cells (IgAk) were incubated with mKap or cKap alone or in the presence of competing free kappa light chains (kBJP). (slideserve.com)
The Hammond lab has a dual focus on engineering nucleic acids as programmable tools for molecular imaging and gene control, and on understanding the chemistry and biology of cyclic dinucleotides as signaling molecules in bacteria and mammalian cells. (utah.edu)
Here, we discuss how research has driven cancer drug development in the past and describe how recent advances in biology, technology, our conceptual understanding of cell networks and removal of some roadblocks may facilitate therapeutic advances in the (hopefully) near future. (biomedcentral.com)
Importantly, vast amounts of information about features distinguishing tumor from normal cells is being accumulated, resulting in frequent, major new insights into cancer biology. (biomedcentral.com)

The antiproliferative effect of compounds was determined by WST-1 test, and nuclear morphology changes in cancer cells stained with Giemsa were evaluated. (japsonline.com)

In this study, anticancer properties of heterocyclic salicylaldimines and their Cu(II) complexes on K562 human chronic myelogenous leukemia cell line were investigated. (ijpsonline.com)

Compounds 3 , 6 and 7 displayed clear activity against K562 (leukemia). (jmcs.org.mx)
The combination of daunorubicin and DNPs were highly effective in killing the multi-drug resistant human leukemia cell K562 (multidrug resistance). (nanouniverse-library.jp)
Leukemia is a blood cancer that develops in the hematopoietic system and presents in the bone marrow with or without peripheral blood cell involvement[ 1 , 2 ]. (ijpsonline.com)
Leukemia cells in patients are intrinsically resistant to standard chemotherapy, and resistance development in leukemia cells toward drugs results in treatment failure[ 4 , 5 ]. (ijpsonline.com)
Three studies have shown that monoclonal antibodies to the DENV fusion loop epitope reliably enhanced Zika virus infection (i.e., antibody-dependent enhancement [ADE]) in Fc receptor-bearing K-562 human myelogenous leukemia cells or the U-937 human monocytic cell line ( 1 - 3 ). (cdc.gov)
Around the same time, and building on the observation that the vitamin folic acid could stimulate acute lymphoblastic leukemia (ALL) cells, Farber used folate analogs such as aminopterin and then amethopterin (methotrexate) to treat ALL, in what is often heralded as the first 'rational' drug development approach [ 4 ]. (biomedcentral.com)

However, the applicability of such traditional chemotherapeutic approaches is primarily limited due to acquired Multi-Drug Resistance (MDR) in cells, which is acquired through the production of anti-apoptotic cascades and the overexpression of cell membrane-based drug efflux pumps, such as P-glycoprotein, for self-defense. (ijpsonline.com)
CSN5 knockdown caused mitotic defects, G2/M arrest and apoptotic cell death. (biomedcentral.com)
CSN2 knockdown resulted in non-apoptotic cell death associated with accumulation of both the autophagy marker LC3-II and autophagic vacuoles. (biomedcentral.com)

In contrast, knockdown of BRD4 but not of BRD2 impairs NK cell cytolytic responses, suggesting BRD4 as critical regulator of NK cell mediated tumor cell elimination. (frontiersin.org)
Attachment of fluorescent dyes neither impaired binding to the TfRs nor affected internalization of the 26 nm-sized VLPs into several human tumor cell lines. (biomedcentral.com)

Here we show that the murine antibody, mKap, binds specifically to a range of kappa-type multiple myeloma (MM) cell lines and mediates in vivo anti-tumor activity in a SCID mouse human myeloma xenograft model. (slideserve.com)
Cell-permeable and active in vivo. (csnpharm.cn)
Among these derivatives, 8a exhibited more than 10-fold antiproliferation activity in several tumor cells than KX01 and significantly improved in vivo antitumor effects. (mirnainhibitors.com)

Although a large burst of reactive oxygen species (ROS) is known to lead to cell damage, a moderate release of ROS from the mitochondria, which occurs during nonlethal short episodes of ischemia, can play a significant triggering role in the signal transduction pathways of ischemic preconditioning leading to reduction of cell damage. (wikipedia.org)
The tyrosine kinase Src is a member of a family of related kinases known as the Src family kinases (SFKs) that share a common structural organization and function as key regulators of signal transduction pathways triggered by a wide variety of surface receptors, including receptor tyrosine kinases, integrins, G-protein-coupled receptors, and antigen receptors (Thomas and Brugge 1997). (mdm2-receptor.com)

It has antioxidant property and inhibites HIV-1 replication in MT-2 cell culture at non-toxic concentrations, a potent and highly selective class of HIV-1 integrase inhibitors. (csnpharm.cn)
Treatment of vector control K562 cells with the autophagy inhibitors 3-methyladenine and bafilomycin A1 recapitulated the growth kinetics, vacuolar morphology and LC3-II accumulation of CSN2 knockdown cells indicating that the cellular phenotype of CSN2 cells arises from autophagy inhibition. (biomedcentral.com)

However, because sufficient specific cell markers are scarce, dyserythropoietic diseases are challenging to identify through flow cytometry. (biomedcentral.com)
Based on the differential expression levels of cell-surface molecules during erythropoiesis, flow cytometry analysis is a useful tool for analyzing the erythropoiesis process [ 8 ]. (biomedcentral.com)

The cytotoxic activity of compounds 1-3 , 6 and 7 was evaluated against selected cancer cell lines. (jmcs.org.mx)
Compounds (C1-8 and doxorubicin) were placed to 96-well culture plate in triplicate order at doses of 1, 10, 100, 1000 μM and then K562 cells were seeded into the wells at the dose of 105/ml. (ijpsonline.com)
Mitochondrial membrane potential was found at low levels in cells treated with Schiff base compounds and doxorubicin. (ijpsonline.com)
Four types of molecular descriptors were generated in other to know the relationship that exit between anti-ulcer activity and structural properties of these compounds. (ufv.br)
Shallangwa, G. Insilico Modelling of Quantitative Structure-Activity Relationship of Pgi50 Anticancer Compounds on k-562 Cell Line. (ufv.br)
Among tested compounds β-iminophosphonate '(E,Z)(3-Methyl-2-(pyridin-2-ylimino)butyl) diphenylphosphine oxide (4e) showed anticancer activity against human fibroblastoma cell line HT 1080. (japsonline.com)

Whereas integrins on circulating PMNs primarily exist in a nonadhesive basal state numerous activators including bacterial products such as fMLP and tissue factors such as TNF-α rapidly increase the cell surface density of Mac-1 and its affinity for extracellular ligands including sites on endothelial cells that collection the blood vessel interior (11). (researchdataservice.com)

Their function is regulated by the activation of a number of activating and inhibitory receptors that bind to specific ligands expressed on the surface of target cells. (frontiersin.org)
In particular, NK cells mediate their cytolytic function through the engagement of activating receptors, such as NKG2D, DNAM-1, NKp30, NKp46, and NKp44 ( 3 , 4 ), or following pro-inflammatory cytokine stimulation ( 5 ). (frontiersin.org)
Interestingly, there exists a subset of viruses with natural affinity for receptors on tumor cells that could be exploited for nanotechnology applications. (biomedcentral.com)
For example, the canine parvovirus (CPV) utilizes transferrin receptors (TfRs) for binding and cell entry into canine as well as human cells. (biomedcentral.com)
Both BLM and A5 are hydrophilic molecules that depend on transporters or endocytosis receptors to get inside of cells. (lookformedical.com)

Furthermore, cKap satisfies the therapeutic criteria of being able to trigger ADCC MMusing human peripheral blood-derived effector cells. (slideserve.com)
γδ T cells recruited into the tumor microenvironment can act as effector cells to mediate cancer immune surveillance. (explorationpub.com)

The dichloromethane-MeOH extract of the aerial parts displayed cytotoxic activity against certain cancer cell lines. (jmcs.org.mx)
We applied NeoLoopFinder in Hi-C data from 50 cancer cell lines and primary tumors and identified tens of recurrent genes associated with enhancer hijacking. (nature.com)
Fig. 2: Detection of neoloops in 50 cancer cell lines or patient samples. (nature.com)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
In the present study, using a facile, low-cost, and solution-based method, we developed and synthesized fucoidan, a natural polymer isolated from seaweed that has demonstrated anti-cancer effect, and coated NPs with it as an ideal candidate in chemo-photothermal therapy against cancer cells. (oncotarget.com)
Fucoidan-coated copper sulfide nanoparticles (F-CuS) act not only as a nanocarrier to enhance the intracellular delivery of fucoidan but also as a photothermal agent to effectively ablate different cancer cells (e.g. (oncotarget.com)
they target tumor cells through the phagocytosis or endocytosis of NPs, and therefore, they are suitable for the delivery of chemotherapeutics in cancer treatment [ 1 ]. (oncotarget.com)
Since the natural products show an efficient anti-cancer effect without imposing any toxicity on normal cells, they are promising as an alternative to chemical drugs [ 2 ]. (oncotarget.com)
Stabilization of quadruplex architecture by small molecules is thus emerging as a potential anticancer approach since it is thought to interfere with oncogenic expression and telomeric maintenance in cancer cells [ 11 - 13 ]. (hindawi.com)
Distinct functions of macrophage-derived and cancer cell-derived cathepsin Z combine to promote tumor malignancy via interactions with the extracellular matrix. (uni-freiburg.de)
It is widely accepted, although clouded by the secrecy of war [ 2 ], that the first tentative steps to treating cancer with drugs emanated from the observation that exposure to chemical warfare agents ('poison gases'), such as nitrogen mustards, could limit the proliferative nature of rapidly dividing lymphoid cells. (biomedcentral.com)
Specific targeting of tumor cells is an important goal for the design of nanotherapeutics for the treatment of cancer. (biomedcentral.com)
In recent studies, this linker modified analog of goniothalamin was shown to be cytotoxic against PC-3 (prostate cancer) and MCF-7 (human breast cancer) cell lines with half maximal inhibitory concentration values of 0.13 Î¼M and 2.6 Î¼M, respectively. (lookformedical.com)

In such cases, stem cell transplant therapy, which is limited by donor availability, is the only proven cure for the patients. (eurekaselect.com)

Anticancer activity on human fibrosarcoma cell line HT 1080 was assessed. (japsonline.com)
The values of LD50 (lethal dose 50%) calculated from experiments are different for each cell line . (lookformedical.com)

Mutations in the Neuraminidase-Like Protein of Bat Influenza H18N11 Virus Enhance Virus Replication in Mammalian Cells, Mice, and Ferrets. (cdc.gov)
Cyclic dinucleotides are an emerging class of intracellular signaling molecules with diverse roles in bacteria and with newfound roles in innate immunity in mammalian cells. (utah.edu)
We recently developed a biosensor-based platereader assay to quantitate cGAMP levels in DNA-stimulated mammalian cell lysates. (utah.edu)

Activated γδ T cells exhibit strong cytotoxic activity and cytokine secretion functions and are effective antitumor lymphocytes with simple and direct recognition modes and rapid responses. (explorationpub.com)

Following activation, NK cells mediate killing of target cells through two major pathways that require direct contact between NK cells and their target cells ( 6 ). (frontiersin.org)

Mitochondria are double membrane-bound organelles of endosymbiotic origin that produce most of the ATP in eukaryotic cells through oxidative phosphorylation (OXPHOS) ( Mitchell, 2011 ). (biorxiv.org)
After cell sorting, the gene expressions of membrane proteins in early and late erythropoiesis precursors and in nonerythroid cells were characterized using microarray analysis. (biomedcentral.com)
To form mature RBCs, the terminal maturation of erythroid cells involves two steps: (1) the condensation and expulsion of the nucleus to form reticulocytes and (2) the organelle clearance and remodeling of the membrane and proteome [ 1 , 7 ]. (biomedcentral.com)

Using a chemo-genetic approach, we identify BET bromodomain containing proteins BRD2 and BRD4 as central regulators of NK cell functions, including direct cytokine secretion, NK cell contact-dependent inflammatory cytokine secretion from monocytes as well as NK cell cytolytic functions. (frontiersin.org)
The testosterone secretion from TM3 cells was measured using the enzyme-linked immunosorbent assay. (lookformedical.com)

This approach was based on the assumption that the deregulation of a protein was causally linked to a disease state, and the pharmacological intervention through inhibition of the deregulated target was able to restore normal cell function. (biomedcentral.com)
suggests that PCNA inhibition Pexidartinib supplier by p21Cip1 is probably not the cause of proliferative unresponsiveness in these Th1 cells. (syksignaling.com)
p21Cip1 in anergic Th1 cells instead appears to work via the inhibition of MAPK, specifically p-JNK and p-c-jun. (syksignaling.com)
We have 'watched' the chemical inhibition of an enzyme involved in biosynthesis of a quorum signaling molecule in live bacterial cells. (utah.edu)
Binding mode and structural elements of Bcr-Abl inhibition are discussed with emphasis on pathways involved in this complex disease to determine alternative strategies and combination therapies. (eurekaselect.com)
These cells may be a key factor ultimately mediating the deviation of the antagonistic response between tumor inhibition and tumor promotion. (explorationpub.com)

It is a mode of cell death defined by characteristic morphological, biochemical and molecular changes. (wikipedia.org)
Consequently, RSTS patient-derived primary cells show decreased levels of HSF2 and HSF2-dependent alteration in their repertoire of molecular chaperones and stress response. (scilifelab.se)
Moreover, we unravel a CBP/EP300-HSF2-N-cadherin cascade that is also active in neurodevelopmental contexts, and show that its deregulation disturbs neuroepithelial integrity in 2D and 3D organoid models of cerebral development, generated from RSTS patient-derived iPSC cells, providing a molecular reading key for this complex pathology. (scilifelab.se)
Heat shock has provided an excellent model to investigate nascent transcription in stressed cells, but the molecular mechanisms orchestrating RNA synthesis during other types of stress are unknown. (scilifelab.se)

Since most of the established VBNPs do not have any specificity for tumor cells and therefore need to be either genetically or chemically modified in order to achieve targeted delivery. (biomedcentral.com)

add a lymphoid cell range from a DBA individual, and fetal liver organ cells and K562 cells transduced with RPS19 shRNA. (acancerjourney.info)
Lymphoid cells have already been suggested to are likely involved in DBA pathophysiology but additional studies didn't demonstrate significant influence of the cells15. (acancerjourney.info)

Eukaryotic cells destroy many endocytosed pathogens by targeting them to the lysosome. (elifesciences.org)

Towards this goal, CPV virus-like particles (VLPs) produced by expression of the CPV-VP2 capsid protein in a baculovirus expression system were examined for attachment of small molecules and delivery to tumor cells. (biomedcentral.com)

However, a single heat exposure in embryonic fibroblasts primed a faster gene induction in their daughter cells by increasing promoter-proximal Pol II pausing and by accelerating the pause release. (scilifelab.se)

Non-human primates (NHPs) can sustain viral replication in relevant cell types and develop a robust immune response, but they do not develop overt disease. (mdpi.com)

First, γδ T cells exposed to complicated cytokine networks are potentially affected by multiple inhibitory mechanisms. (explorationpub.com)

However, with the exception of idiotype, few antigen targets have been identified that would facilitate specific immunotherapy of MM. We have previously described a murine monoclonal antibody that recognizes a conformation-dependent epitope on free human kappa light chains and a cell surface antigen, KMA, expressed on kappa MM (MM) plasma cells 1,2. (slideserve.com)
In T cells, productive antigen stimulation triggers the activation of MAPK including extracellular signal-regulated kinase, p38 and JNK.33 The JNK is activated through the dual phosphorylation of its Thr and Tyr residues by mitogen-activated kinase kinase Pembrolizumab 4 (MKK4) and MKK7. (syksignaling.com)

activity in anergic T cells.18-20,35 Depsipeptide solubility dmso In accordance with these earlier studies, c-fos and c-jun activity was decreased in Th1 cells anergized by exposure to n-butyrate. (syksignaling.com)
We are one of the first labs to develop fluorescent biosensors made of RNA for live cell imaging of enzyme activity. (utah.edu)

Given the important role of both cytokine-mediated inflammatory microenvironment and cytolytic NK cell activities in immune-oncology therapies, our findings present a compelling argument for further clinical investigation. (frontiersin.org)
Therefore, an in-depth understanding of γδ T cells that play conflicting roles in the tumor microenvironment is necessary. (explorationpub.com)

FTY720 Phosphate is a derivative of ISP-1 that is a fungal metabolite as well as a structural analog of sphingosine. (csnpharm.cn)

These variable loops contribute significantly to corresponding gene expression variability across cells and species, possibly by refining sub-TAD-scale loop contacts responsible for cell-type-specific enhancer-promoter interactions. (nature.com)
As second messengers, they are produced transiently in response to environmental stimuli and act inside the cell to change gene expression, physiology, and behavior. (utah.edu)

Early observations revealed that individual chromosomes occupy distinct nuclear territories and, while their arrangement varies between different cell types, this structure is conserved between mother and daughter cells 2 . (nature.com)

Programmed cell death is a distinct genetic and biochemical pathway essential to metazoans. (wikipedia.org)
It plays an important role during embryonal development as programmed cell death and accompanies a variety of normal involutional processes in which it serves as a mechanism to remove "unwanted" cells. (wikipedia.org)

Natural killer (NK) cells are innate lymphocytes that play a pivotal role in the immune surveillance and elimination of transformed or virally infected cells. (frontiersin.org)
Natural killer cells are cytolytic lymphocytes belonging to the innate immune system and are involved in anti-viral and anti-tumor responses ( 1 ) and are recognized as major players in immune-mediated anti-tumor therapies ( 2 ). (frontiersin.org)
Moreover, NK cells are poised to release cytokines such as IFN- γ , TNF-α and growth factors that can initiate inflammatory responses mediated by both the innate and the adaptive arm of the immune system. (frontiersin.org)
This review further emphasizes the diversified cross-talk between γδ T cells and other immune cells. (explorationpub.com)
It is hoped that the host immune status can be accurately predicted and gradually advance γδ T cell precise individualized medicine. (explorationpub.com)

Characterization of large structural genetic mosaicism in human autosomes. (cdc.gov)

HSF1 binds to many chaperone genes upon oxidative and heat stress but activates them only in heat-shocked cells. (scilifelab.se)

Once inside, the anticancer activities rely on their abilities to produce DNA breaks, thus leading to cell death. (lookformedical.com)

However, a growing body of evidence currently testifies that this canonical association is not the unique mode of stabilization of G-rich DNA in cells. (hindawi.com)

We show that pause-release of RNA polymerase II (Pol II) is a universal mechanism regulating gene transcription in stressed cells, while enhancers are activated at the level of Pol II recruitment. (scilifelab.se)
Tracking transcription genome-wide at nucleotide-resolution revealed that cells precisely restored RNA polymerase II (Pol II) distribution at gene bodies and enhancers upon recovery from stress. (scilifelab.se)
To experimentally validate NeoLoopFinder, we deleted the hijacked enhancers in prostate adenocarcinoma cells using CRISPR-Cas9, which significantly reduced expression of the target oncogene. (nature.com)

Western blot analysis revealed that conditioned medium from 12-O-tetradecanoylphorbol-13-acetate (TPA)-treated, megakaryocytic differentiating K562 cells upregulated DAB2 expression. (edu.hk)
However, production of VLPs in adequate quantities has been achieved by expression of virus capsid proteins in heterologous systems (insect cells, yeast, and bacteria). (biomedcentral.com)

This study aimed to explore the role and mechanism of action of sulforaphane (SFN) in protecting mouse Leydigs (TM3) cells from cadmium (Cd)-induced damage. (lookformedical.com)

In comparison with already published PKA and Pim1 binders, we could detect either known but also new structural scaffolds for the interactions with the protein. (uni-marburg.de)
Taking advantage of the diverse γδ TCR repertoire or other ligand-receptor interactions, γδ T cells can recognize a broad spectrum of tumor-associated antigens (TAAs) in a major histocompatibility complex (MHC)-independent manner, thereby activating downstream pleiotropic effects. (explorationpub.com)

Anatomy28

Organisms21

Diseases4

Chemicals and Drugs114

Analytical, Diagnostic and Therapeutic Techniques and Equipment28

Phenomena and Processes62

Disciplines and Occupations4

Information Science3

Health Care2

Apoptosis15

Erythroleukemia cells2

Vitro4

HeLa4

Leukemic2

Myeloid2

Functional11

Inhibitor2

Subunits3

Chromatin4

Proliferation2

IC502

Subunit2

Biology4

Antiproliferative1

Complexes1

Leukemia6

Apoptotic3

Tumor cell2

Vivo3

Transduction2

Inhibitors2

Flow cytometry2

Compounds6

Endothelial cells1

Receptors5

Effector cells2

Cancer13

Stem cell trans1

Line2

Mammalian3

Cytotoxic activity1

Mediate1

Membrane3

Secretion2

Inhibition6

Molecular4

Specificity1

Lymphoid2

Eukaryotic1

Small molecules1

Induction1

Replication1

Inhibitory1

Antigen2

Activity2

Microenvironment2

Fungal1

Gene expression2

Nuclear1

Programmed cell2

Immune5

Genetic1

Oxidative1

Anticancer1

Stabilization1

Enhancers3

Expression2

Cadmium1

Interactions2