• An inoculum from a pure culture is transferred aseptically to a tube of OF glucose medium with oil overlay The agar is stabbed through the oil to "plant" the bacteria in the butt of the tube. (vumicro.com)
  • In fact, all strains of Group D Streptococci hydrolyze æsculin in 40% bile. (wikipedia.org)
  • All 4 isolates grew poorly in air and 5% CO 2 , were catalase negative, did not hydrolyse esculin or gelatin, or reduce nitrates. (cdc.gov)
  • tests ability of the organism to hydrolyze esculin to esculetin. (umd.edu)
  • The 4 isolates did not ferment most of the carbohydrates in the 2 API kits, except for ribose, maltose, glucose, and sucrose. (cdc.gov)
  • Commercial systems, such as analytical profile index (API) Rapid ID 32 Strep and Coryne kits (bioMérieux, Marcy l'Etoile, France), used in differentiation of novel bacteria provide biochemical profiles useful for identification of V . cambriense ( 1 ). (cdc.gov)
  • Group B Streptococci can be identified by the "camp effect" by making a single streak of the unknown bacteria perpendicularly to a strain of Staphylococcus aureus that is known to be crystal violet resistant and produces βeta-Lysin. (hylabs.co.il)
  • It favors growth of Beta haemolytic Streptococci ensuring luxuriant growth of the most fastidious microorganisms within 24 hours at 37oC (anaerobically) while the absolutely unique combination of microbial agents contained in the medium inhibits Gram negative and Gram positive bacteria except for Beta - haemolytic Streptococci which are not affected, and can be differentiated by their typical haemolytic reaction. (hylabs.co.il)
  • P. fontium strains give positive tests for Simmons citrate, H2S production, motility, acid production from D-glucose and D-galactose, and gluconate oxidation. (who.int)
  • The majority of strains are positive in tests for methyl red and esculin. (who.int)
  • Aesculin, also called æsculin or esculin, is a coumarin glucoside that naturally occurs in the trees horse chestnut (Aesculus hippocastanum), California buckeye (Aesculus californica), prickly box (Bursaria spinosa), and daphnin (the dark green resin of Daphne mezereum). (wikipedia.org)
  • Group D Streptococci are capable of hydrolyzing Aesculin to glucose and aglycone 6-7 dihydroxycumarin: The aglycone reacts with the iron salts in the medium giving them a black coloring. (hylabs.co.il)
  • The 4 unidentified gram-positive bacilli from these patients were initially characterized by using the API Rapid ID 32 Strep and Coryne kits. (cdc.gov)
  • Highly selective due to the addition of special inhibitors making it highly selective for Streptococci, particularly when Gram negative bacilli (e.g. (hylabs.co.il)
  • Two tubes of OF glucose medium are used, one of which is covered with a layer of sterile mineral oil as an oxygen barrier. (vumicro.com)
  • A selective enriched medium for the isolation of haemolytic Streptococcus from throat swabs and other clinical specimens. (hylabs.co.il)
  • to perform CAMP test for Group B streptococci. (umd.edu)
  • A zone of growth inhibition around a bacitracin (0.04 units) disc allows the specific differentiation of group A Beta haemolytic Streptococci. (hylabs.co.il)
  • A positive result for fermentation of glucose is indicated by the medium turning yellow. (vumicro.com)
  • Zymase is a complex of enzymes found in yeast cells that catalyze the conversion of sugars, such as glucose, into alcohol (ethanol) and carbon dioxide during the process of alcoholic fermentation. (labtestsguide.com)
  • For example, a throat culture is taken by scraping the lining of tissue in the back of the throat and blotting the sample into a medium to be able to screen for harmful microorganisms, such as Streptococcus pyogenes , the causative agent of strep throat. (atozwiki.com)
  • i.e., the beta-hemolytic streptococci and the pneumococci. (cdc.gov)
  • All 4 isolates grew poorly in air and 5% CO 2 , were catalase negative, did not hydrolyse esculin or gelatin, or reduce nitrates. (cdc.gov)
  • The same procedure was repeated for all the tubes while at the same time avoiding the Durham tube when the Glucose broth is being inoculated. (topessaywriting.org)
  • The medium used is MRVP broth , a nutrient broth medium with 0.5% glucose added. (vumicro.com)
  • By hospital day 7, the organism was identified by the API 20 Strep System (bioMerieux, Marcy l'Etoile, France), as S . suis serotype 2. (cdc.gov)
  • Commercial systems, such as analytical profile index (API) Rapid ID 32 Strep and Coryne kits (bioMérieux, Marcy l'Etoile, France), used in differentiation of novel bacteria provide biochemical profiles useful for identification of V . cambriense ( 1 ). (cdc.gov)
  • The 4 isolates did not ferment most of the carbohydrates in the 2 API kits, except for ribose, maltose, glucose, and sucrose. (cdc.gov)
  • An important component of the medium is a modest amount of glucose, necessary for the process to proceed. (vumicro.com)
  • The 4 unidentified gram-positive bacilli from these patients were initially characterized by using the API Rapid ID 32 Strep and Coryne kits. (cdc.gov)