• Out of 31 clinical isolates of Staphylococcus spp. (nuthealth.org)
  • In this study, a large number of virulence factors were examined in S. aureus nasal isolates, and the data obtained from this study can be used for monitoring the prevalence of virulence genes in S. aureus strains isolated from nasal carriers. (balkanmedicaljournal.org)
  • Isolates of S. aureus are generally susceptible to β-lactam antibiotics, but extensive use of this class of drugs has led to increasing emergence of resistant strains. (biomedcentral.com)
  • Because the occurrence of fully vancomycin-resistant staphylococcal infection in a hospital could result in serious public health consequences, CDC and the Hospital Infection Control Practices Advisory Committee have developed interim guidelines to direct medical and public health responses when isolates of staphylococci with reduced vancomycin susceptibility are identified. (cdc.gov)
  • Among isolates of the genus Staphylococcus, a typical representative of starter cul- tures for meat, there prevailed: S. xylosus (5.56 log10CFU/g), S warneri (5.36 log10CFU/g), S. lentus (3.30 log10CFU/g) and S. auricula- ris (3.30 log10CFU/g). (srce.hr)
  • Cefoxitin (30g) was used as surrogate to determine phenotypic methicillin resistance in staphylococcus isolates, and the methicillin resistance ( mec A) gene was detected by conventional PCR assay. (who.int)
  • Suspected Staphylococcus isolates were identified initially using standard biochemical tests, then strains that were confirmed by polymerase chain reaction (29 S. aureus and 17 S. saprophyticus ) were evaluated for their susceptibility to different antimicrobials. (who.int)
  • In total, 114 217 test results were included for the clinical isolates, and 3582 test results for control strains. (lu.se)
  • CA-MRSA, community-associated methicillin-resistant Staphylococcus aureus . (cdc.gov)
  • MRSA strains may spread in all geographic regions between different ecological niches resulting in major healthcare costs [ 2 - 4 , 6 , 9 , 10 , 17 ] and relevant economic losses in the food animal industry [ 2 , 11 , 15 - 17 ]. (hindawi.com)
  • ABSTRACT We describe and validate a novel PCR assay to detect the pandemic hospital-acquired methicillin-resistant Staphylococcus aureus (HA-MRSA) lineage ST 239. (tropmedres.ac)
  • Some Staphylococcus species are frequently recognized as etiological agents of many animal and human opportunistic infections This is the first report testing the antibiotic resistance-modifying activity of Turnera ulmifolia against methicillin-resistant Staphylococcus aureus - MRSA strain. (springer.com)
  • In this study an ethanol extract of Turnera ulmifolia L. and chlorpromazine were tested for their antimicrobial activity alone or in combination with aminoglycosides against an MRSA strain. (springer.com)
  • It is therefore suggested that extracts from Turnera ulmifolia could be used as a source of plant-derived natural products with resistance-modifying activity, constituting a new weapon against the problem of bacterial resistance to antibiotics demonstrated in MRSA strains. (springer.com)
  • The combination of the P. guineense extract with the antimicrobial agents resulted in an eight-fold reduction in the MIC of these agents, which showed a FICI ranging from 0.125 to 0.5, suggesting a synergistic interaction against methicillin-resistant Staphylococcus aureus ( MRSA ) strains . (bvsalud.org)
  • This study demonstrated that the aqueous extract of P. guineense combined with beta lactamics antimicrobials, fluoroquinolones , and carbapenems , acts synergistically by inhibiting MRSA strains . (bvsalud.org)
  • In many parts of the world, epidemiologically and genotypically defined community-associated (CA) methicillin-resistant Staphylococcus aureus (MRSA) strains have emerged to become frequent causes of hospital infection. (ox.ac.uk)
  • The aim of this study was to use spatial models with adjustment for area-level hospital attendance to determine the transmission niche of genotypically defined CA- and health-care-associated (HA)-MRSA strains across a diverse region of South East London and to explore a potential link between MRSA carriage and markers of social and material deprivation. (ox.ac.uk)
  • CONCLUSIONS: There was no evidence of community transmission of HA-MRSA strains, implying that HA-MRSA cases identified in the community originate from the hospital reservoir and are maintained by frequent attendance at health care facilities. (ox.ac.uk)
  • Staphylococcus aureus is a predominant pathogen in keratitis, and the rate of methicillin-resistant S. aureus (MRSA) is increasing. (elsevierpure.com)
  • The emergence of methicillin-resistant Staphylococcus aureus (MRSA) is a main concern in burn care centers worldwide. (biomedcentral.com)
  • Dru typing can be used to differentiate MRSA strains with SCC mec IIIA. (biomedcentral.com)
  • Preliminary analysis differentiating MRSA from MSSA demonstrated the feasibility of simultaneous determination of strain identification and antibiotic resistance. (biomedcentral.com)
  • Methicillin-resistant Staphylococcus aureus (MRSA) is the name for the strains of staph bacteria … cause more serious infections like sepsis. (wisconsin.gov)
  • Resources Resources for individuals and families Methicillin-resistant Staphylococcus aureus (MRSA) Frequently Asked Questions (FAQ) : FAQ for patients and family members of patients with MRSA. (wisconsin.gov)
  • ABSTRACT We evaluated the utility of 2 methods for detection of methicillin-resistant Staphylococcus aureus (MRSA) directly from signal-positive blood culture bottles: loop-mediated isothermal amplification (LAMP) assay, and direct cefoxitin disk diffusion (DCDD) test using a 30 g cefoxitin disk. (who.int)
  • This cellulitis was caused by community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA). (medscape.com)
  • Methicillin-resistant Staphylococcus aureus (MRSA) strains formed 25of this species. (bvsalud.org)
  • The new action plan sets specific reduction goals for many resistant strains, such as reducing MRSA bloodstream infections by 50 percent by 2020. (medscape.com)
  • MEFOXIN is indicated for the treatment of serious infections caused by susceptible strains of the designated microorganisms in the diseases listed below. (rxlist.com)
  • Clarithromycin and erythromycin are macrolide antimicrobials indicated for the treatment of infections caused by susceptible strains of designated microorganisms. (medscape.com)
  • Staphylococcus epidermidis is a Gram-positive bacterium, and one of over 40 species belonging to the genus Staphylococcus. (wikipedia.org)
  • The Staphylococcus genus is composed of 44 species, with S. aureus being the most pathogenic. (biomedcentral.com)
  • Leave-one-spectrum-out cross-validation indicated 100 % correct assignment at the species and strain level. (biomedcentral.com)
  • The utility of CeO 2 -MOLI MS FA profiling coupled with multivariate statistical analysis for performing strain-level differentiation of various Staphylococcus species proved to be a fast and reliable tool for identification. (biomedcentral.com)
  • Staphylococci are Gram-positive facultative anaerobes comprising 44 species commonly found in soil or on the skin of animals [ 1 ]. (biomedcentral.com)
  • After repeat testing, if species identification and vancomycin test results are consistent, immediately contact the state health department (SHD) and CDC's Hospital Infections Program, National Center for Infectious Diseases, telephone (404) 639-6400, to report the occurrence of a 'presumptive' staphylococcal strain with reduced susceptibility to vancomycin and to obtain epidemiologic and laboratory assistance. (cdc.gov)
  • All S. aureus strains were susceptible to P. guineense as determined by the microdilution method . (bvsalud.org)
  • Although 5 strains were phenotypically resistant to penicillin, the other 5 (all belonging the clonal complex 8) were penicillin susceptible. (gstsvs.ch)
  • Using the genetically altered blaZ genes detected as targets, a real-time PCR system for detecting CC8 associated blaZ positive S. aureus strains that still remain susceptible to penicillin was developed. (gstsvs.ch)
  • The normal distribution of the ideal population of susceptible strains was then determined by using the least-squares method for probit values against zone diameters, and the ideal population was thereby. (lu.se)
  • ulase-negative staphylococci (CoNS) need for a thermocycler, the speed of Approval to perform the study was and methicillin-susceptible strains has the reaction and visual read-out make obtained from the ethics committee in significant prognostic, therapeutic and LAMP a promising platform for the the Faculty of Medicine, Suez Canal economic value. (who.int)
  • It is sensitive to novobiocin, providing an important test to distinguish it from Staphylococcus saprophyticus, which is coagulase-negative, as well, but novobiocin-resistant. (wikipedia.org)
  • RÉSUMÉ La présente étude a évalué la résistance aux antimicrobiens, après caractérisation moléculaire, de souches de Staphylococcus aureus et S. saprophyticus isolées à partir de trois produits libanais dérivés du lait, parfois consommés crus : le kishk , le shanklish et le fromage baladi . (who.int)
  • Des isolats suspects de Staphylococcus ont d'abord été identifiés par l'analyse biochimique classique, puis les souches confirmées par amplification en chaîne par polymérase (29 isolats de S. aureus et 17 isolats de S. saprophyticus ) ont été analysées pour déterminer leur sensibilité aux différents antimicrobiens. (who.int)
  • 1].Unpasteurizedmilkmaybecome S. saprophyticus in baladi cheese, kishk able, unsatisfactory and potentially contaminated with enterotoxigenic and shankleesh .Identificationofthese hazardous.Inthecaseof Staphylococcus coagulase-positive Staphylococcus spp. (who.int)
  • More than half of the strains (54%) were resistant to penicillin, 29.4% to tetracycline, 23.5% to erythromycin, and 17% showed resistance to ciprofloxacin. (hindawi.com)
  • Staphylococcus aureus is an opportunistic pathogen related to a variety of life-threatening infections but for which antimicrobial resistance is liming the treatment options. (nature.com)
  • Four S. aureus strains showed resistance to all antimicrobial agents tested and were selected for the study of the interaction between aqueous extract of P. guineense and antimicrobial agents , by the checkerboard method . (bvsalud.org)
  • Molecular based approaches have gained increasing importance in routine mastitis diagnostics for typing and antibiotic resistance testing of Staphylococcus aureus. (gstsvs.ch)
  • Such strains are part of detection challenges that must be considered in routine application of genotypic resistance testing of bovine mastitis S. aureus. (gstsvs.ch)
  • Increased occurrence of coagulase-negative staphylococci as well as S. aureus infections , some with resistance to multiple classes of antibiotics, has driven the necessity for innovative options for treatment and infection control. (biomedcentral.com)
  • This study describes the use of metal oxide laser ionization mass spectrometry fatty acid (FA) profiling as a rapid, simultaneous Staphylococcus identification and antibiotic resistance determination method. (biomedcentral.com)
  • The simultaneous strain-level detection and antibiotic resistance determination achieved with this method should greatly improve outcomes and reduce clinical costs for therapeutic management and infection control. (biomedcentral.com)
  • with antibiotics, but there are some strains that have developed antibiotic resistance. (wisconsin.gov)
  • We retrospectively investigated the bacteria from endophthalmitis samples (aqueous humor and vitreous) and found that the three organisms most commonly responsible are Staphylococcus spp . (molvis.org)
  • Staphylococcus epidermidis is a bacteria that thrives in warm, moist environments and is a common bacteria in the human microbiome. (wikipedia.org)
  • It is this bacteria that is primarily responsible for foot odor because feet have more sweat glands than any other part of the body and thus are often moist, which creates an ideal environment for Staphylococcus epidermidis to live in. (wikipedia.org)
  • Furthermore, as a part of the indigenous microbial population and potential starter cultures that are responsible for a certain avor and texture of traditional meat products, except for lactic acid bacteria, bacteria of the genus Staphylococcus were isolated. (srce.hr)
  • Staphylococcus aureus is the most dangerous of all of the many common staphylococcal bacteria. (msdmanuals.com)
  • Staphylococcus aureus is an important human opportunistic pathogen involved in a wide range of human infections. (nature.com)
  • In this study, we identified the intrinsic resistome to a broad spectrum of antimicrobials in the human pathogen, Staphylococcus aureus . (frontiersin.org)
  • TAE6080, a possibly new bacterium isolated in 1994 during water column samplings near the French Antarctic station Dumont d'Urville, is capable of inhibiting the formation of Staphylococcus epidermidis biofilm, known to be an important opportunistic pathogen in infections associated to medical devices. (unisa.it)
  • Staphylococcus cohnii has become increasingly recognized as a potential pathogen of clinically significant nosocomial and farm animal infections. (pacb.com)
  • The virulence-associated three synergistic hemolysins have been identified in the strain.To the best of our knowledge, this is the first complete genome of S. cohnii, and will provide important insights into the biodiversity of CoNS and valuable information for the control of this emerging pathogen. (pacb.com)
  • Biochemical characterization of clinical strains of staphylococcus spp. (nuthealth.org)
  • Some of the most common approaches for analysis of the specific biochemical characteristics of staphylococci include: coagulase and phosphatase activity, hemolysis, nitrate reduction, and aerobic acid production from carbohydrate metabolism [ 6 ]. (biomedcentral.com)
  • Kloos and coworkers reported a simplified scheme for analyzing the extensive data produced by biochemical results to characterize staphylococci. (biomedcentral.com)
  • Here we report the biochemical features of the Staphylococcus aureus NDK and the mutant enzymes. (rcsb.org)
  • Staphylococcus genus is widely spread in nature being part of the indigenous microbiota of skin and mucosa of animal and birds. (springer.com)
  • In this article, we describe for the first time the high-resolution crystal structure of a phenylalanine tRNA synthetase from the pathogenic bacterium Staphylococcus haemolyticus. (rcsb.org)
  • The evolution of S. aureus in the antibiotic era has revealed the emergence of virulent strains, many of which include acquisition of antibiotic resistant to methicillin [ 15 , 16 ]. (hindawi.com)
  • Staphylococcus aureus infections range from mild to life threatening. (msdmanuals.com)
  • To date, the mechanisms of biofilm formation are not well understood, therefore the purpose of this study was to determine the relationship between the adhesive properties, the level of teichoic acids and the ability to form biofilms of clinical and reference strains of Staphylococcus aureus. (scholarsresearchlibrary.com)
  • The method relies on well standardized disk tests, but is independent of differences in MIC limits and zone breakpoints, and does not require the use of reference strains. (lu.se)
  • Colonization of mice with an S. epidermidis strain producing 6-HAP reduced the incidence of ultraviolet-induced tumors compared to mice colonized by a control strain that did not produce 6-HAP. (jax.org)
  • Reduction of overuse and misuse of antimicrobials will decrease the risk for emergence of staphylococci with reduced susceptibility to vancomycin. (cdc.gov)
  • Hypertoxin producing strains of C. difficile cause increased morbidity and mortality, as these infections can be refractory to antimicrobial therapy and may require colectomy. (nih.gov)
  • Staphylococcus aureus and coagulase-negative staphylococci are the most common infections in both neonatal and paediatric intensive care units 1,2 and have a significant impact on patients' mortality and morbidity 3-5 . (bvsalud.org)
  • Zone diameter histograms for control strains were first analyzed to validate the procedure, and a comparison of actual means with the calculated means showed a correlation coefficient of r = 0.998. (lu.se)
  • Other than the isolate reported in Japan (4), all Staphylococcus aureus strains with putative reduced vancomycin susceptibility sent to CDC for confirmation have been misidentified or mixed with other microorganisms. (cdc.gov)
  • We screened the Nebraska Transposon Mutant Library of 1920 single-gene inactivations in S. aureus strain JE2, for increased susceptibility to the anti-staphylococcal antimicrobials (ciprofloxacin, oxacillin, linezolid, fosfomycin, daptomycin, mupirocin, vancomycin, and gentamicin). (frontiersin.org)
  • Rare cases of infection in the United States (1) have been caused by coagulase-negative staphylococci with reduced susceptibility to vancomycin (minimum inhibitory concentration {MIC} greater than or equal to 8 ug/mL) * (2). (cdc.gov)
  • Use of recommended laboratory methods (including media and incubation methods, antimicrobial susceptibility testing methods, and susceptibility breakpoints) for identifying such strains is essential. (cdc.gov)
  • The most accurate form of antimicrobial susceptibility testing for staphylococci is a minimal inhibitory concentration method (broth dilution, agar dilution, or agar-gradient diffusion) using a full 24-hour incubation. (cdc.gov)
  • All strains with a MIC greater than or equal to 4 ug/mL should be considered candidate strains for reduced vancomycin susceptibility. (cdc.gov)
  • Furthermore, the isolate S. xylosus SK1 has also shown adhesion properties on epithelial cells of mice intestine and thus satised one of the selection criteria for the selection of probiotic strains. (srce.hr)
  • Thus, the aim of this study was to reveal the antibacterial mechanism of MDA on Staphylococcus xylosus and Lactiplantibacillus plantarum isolated from dry-cured fish. (frontiersin.org)
  • This decreased metabolism, in combination with impaired diffusion of antibiotics, makes it difficult for antibiotics to effectively clear this type of infection.S. epidermidis strains are often resistant to antibiotics, including rifamycin, fluoroquinolones, gentamicin, tetracycline, clindamycin, and sulfonamides. (wikipedia.org)
  • 7 However, during the past two decades, reports from several cholera-endemic countries of strains resistant to antibiotics including tetracycline, ampicillin, kanamycin, streptomycin, sulphonamides, trimethoprim and gentamicin have appeared. (who.int)
  • That threat has manifested in a number of health problems, from methicillin-resistant Staphylococcus aureus to multidrug-resistant tuberculosis, of which there were 450,000 new cases worldwide in 2012. (medscape.com)
  • Staphylococcus aureus is one of the most common causes of skin and soft tissue infection in both the health care and community settings. (cdc.gov)
  • Staphylococci are one of the most common causes of community- and hospital-acquired infection. (cdc.gov)
  • Antibiotics are chosen based on whether they are likely to be effective against the strain causing the infection. (msdmanuals.com)
  • The Food and Drug Administration (FDA) approved vancomycin for use in the United States in 1958 to treat penicillin-resistant1 Staphylococcus aureus infection. (bvsalud.org)
  • Staphylococci produce two types of coagulase: Staphylocoagulase, a free coagulase that produces true clotting of plasma, and Staphylococcal clumping factor , a bound coagulase in the cell wall that induces clumping of cells in the presence of fibrinogen . (online-medical-dictionary.org)
  • Staphylococcal scalded skin syndrome (SSSS) is a rare dermatological condition associated with extensive desquamation by toxicogenic strains of staphylococcus aureus. (amrita.edu)
  • We characterized a number of clinical strains of Staphylococcus spp. (nuthealth.org)
  • Strains of staphylococci with a MIC=8 ug/mL (classified as intermediate using National Committee for Clinical Laboratory Standards breakpoints) were not detected by using the current disk diffusion procedure. (cdc.gov)
  • In order to improve the specificity and selectivity of Staphylococcus detection, molecular methods for analyzing specific genetic markers have been explored. (biomedcentral.com)
  • We examined strains associated with pyomyositis using Bugwas, which identifies bacterial lineages and tests them for association with phenotype. (ox.ac.uk)
  • added volatile aldehydes and ketones extracted from oyster mushroom to bacterial cultures and found that the growth and reproduction of the test strains were completely inhibited. (frontiersin.org)
  • The aim of this study was to evaluate the antimicrobial activity of aqueous extract of Psidium guineense Swartz (Araçá-do-campo) and five antimicrobials ( ampicillin , amoxicillin / clavulanic acid , cefoxitin , ciprofloxacin , and meropenem ) against twelve strains of Staphylococcus aureus with a resistant phenotype previously determined by the disk diffusion method . (bvsalud.org)
  • Out of 78 S. aureus strains isolated from bovine mastitis milk 10 of them harbored blaZ, blaI and blaR genes. (gstsvs.ch)
  • PCR amplification confirmed the presence of the blaZ, blaR and blaI genes in all 5 strains. (gstsvs.ch)
  • Sequencing of these genes uncovered a 29 base deletion within the blaZ gene in all these strains that causes a translational frame shift, which is predicted to induce abrogation of BlaZ expression. (gstsvs.ch)