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These data demonstrated that ME49Δ cdpk3 inoculation induced effective cellular and humoral immune responses against acute and chronic Toxoplasma infections with various strains and was a potential candidate to develop a vaccine against toxoplasmosis. (nature.com)
Thus, the development of an effective toxoplasmosis vaccine is critical for limiting the infection of various Toxoplasma strains. (nature.com)
This project research project seeks to develop an African swine fever virus (ASFV) sub unit vaccine, using raccoon pox virus as vector, which can protect against the epidemiologically relevant strain Georgia 2007. (usda.gov)
Develop an ASFV subunit vaccine candidate using racoon pox as a vector with the co-expression of several (6-18) immunogenetic viral proteins. (usda.gov)
A bivalent meningococcal polysaccharide protein conjugate vaccine that provides protection against meningococcal serogroups C and Y along with Haemophilus influenzae type b (Hib) (Hib-MenCY-TT [MenHibrix, manufactured by GlaxoSmithKline Biologicals, Rixensart, Belgium]) is licensed for use in children aged 6 weeks through 18 months. (cdc.gov)
It is recommended that an authorized protein subunit COVID-19 vaccine (Novavax Nuvaxovid) should be offered to individuals in the authorized age groups without contraindications to the vaccine who are not able or willing to receive an mRNA COVID-19 vaccine. (canada.ca)
Hence, the S protein is the lead target for vaccine development. (news-medical.net)
The researchers paired Baylor's SARS-CoV-2 RBD recombinant protein formulation vaccine with IDRI's aluminum-based formulation of 3M's Toll-like receptor 7 and 8 agonist 3M-052 (3M-052/Alum) to enhance the immune response against SARS-CoV-2, increasing the vaccine's efficacy against COVID-19. (news-medical.net)
The team believes that the vaccine containing a recombinant RBD protein with its novel 3M-052 adjuvant formulation may be potent against SARS-CoV-2, including the newly emerging variants. (news-medical.net)
This information will be used to identify genes that determine viral virulence that could targeted for deletion of mutation in order to yield attenuated viral strains with potential as vaccine candidates. (usda.gov)
The vaccine is based on two fused proteins and acts by making the immune system react against Streptococcus mutans, the bacteria responsible for tooth decay. (wallstreetpit.com)
To lessen this aspect, researchers improved the vaccine with a new protein fusion now referred to as KFD2-rPAc. (wallstreetpit.com)
Cross-reactivity between vaccine antigens from the chitin deacetylase protein family improves survival in a mouse model of cryptococcosis. (umassmed.edu)
A PG-tb1 hapten from the West Beijing strains of Mycobacterium tuberculosis cell wall has been efficiently synthesized and conjugated to CRM197 in a simple way as linker-equipped carbohydrate by applying squaric acid chemistry for an original neoglycoprotein, creating a potent T-dependent conjugate vaccine. (bvsalud.org)
A quadrivalent recombinant influenza vaccine (RIV4) and a cell culture-based vaccine (ccIIV4) that do not contain egg protein are available. (msdmanuals.com)
Recombinant influenza vaccine (RIV4) can be used in people aged 18 to 49 years. (msdmanuals.com)
Adults ≥ 65 years should be given any one of the quadrivalent high-dose IIV, quadrivalent recombinant influenza vaccine, or quadrivalent adjuvanted IIV. (msdmanuals.com)

Hjc_15-6 was heterologously produced in Escherichia coli and high yields of soluble and biologically active recombinant enzyme were obtained in both complex and defined media. (iucr.org)
Contamination by bacterial viruses is one of the most difficult problems to evaluate in the modern biotechnology industry, especially in the productions of recombinant proteins that are based on Escherichia coli strains. (sld.cu)
A protocol for the incorporation of selenomethionine and selenocysteine into recombinant proteins overexpressed in Escherichia coli based on the use of a suitable cysteine auxotrophic strain. (cnrs.fr)

Once purified by successive purification steps, expression of ASFV recombinant proteins will be tested in vitro using monoclonal and monospecific polyclonal antibodies. (usda.gov)
The company also offers a variety of customized services, mainly focusing on recombinant production of antigens and antibodies. (the-scientist.com)
Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies. (antikoerper-online.de)
Western blot and immunofluorescence assay (IFA) were used to examine the reactivity of rFg14-3-3e protein to anti- F. gigantica and anti-rFg14-3-3e antibodies, respectively. (biomedcentral.com)
Western blot analysis showed that rFg14-3-3e protein is recognized by sera from goats experimentally infected with F. gigantica and immunofluorescence staining using rat anti-rFg14-3-3e antibodies demonstrated the specific binding of rFg14-3-3e protein to the surface of goat PBMCs. (biomedcentral.com)
Antibodies directed against B. burgdorferi's outer surface protein C (OspC) are known to block tick-to-host transmission, as well as dissemination of the spirochete within a mammalian host. (cdc.gov)
Initial studies demonstrated that rabbit antimouse type VII collagen antibodies, as well as rabbit antibodies raised against human recombinant protein type VII collagen, were able to induce blisters in mice. (medscape.com)

The company specializes in recombinant antigen production and antibody development. (the-scientist.com)
Its newly launched ProVir™ collection is the world's largest viral antigen bank, carrying over 1,000 viral products from 350 strains of viruses. (the-scientist.com)
PPE68 (Rv3873), a major antignic protein encoded by Mycobacteriun tuberculosis-specific genomic region of difference (RD)1, is a strong stimulator of peripheral blood mononuclear cells (PBMCs) obtained from tuberculosis patients and Mycobacterium bovis bacillus Calmette Guerin (BCG)-vaccianted healthy subjects in T helper (Th)1 cell assays, i.e. antigen-induced proliferation and interferon-gamma (IFN-Î³) secretion. (bvsalud.org)
To confirm the antigen-specific recognition of PPE68 by T cells in IFN-Î³ assays, antigen-induced human T-cell lines were established from PBMCs of M. Bovis BCG-vaccinated and HLA-heterogeneous healthy subjects and tested with peptide pools of RD1 proteins. (bvsalud.org)
Here, we report the crystal structure of B5 antigen-binding fragments (Fabs) in complex with recombinant OspC type A (OspC(A)). Each OspC monomer within the homodimer was bound by a single B5 Fab in a side-on orientation, with contact points along OspC's -helix 1 and -helix 6, as well as interactions with the loop between -helices 5 and 6. (cdc.gov)

Sandwich ELISA detection of recombinant nucleocapsid protein(s) derived from different strains of SARS-CoV-2 virus (ie. (genetex.com)
Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] (GTX635685) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) as detection antibody at concentration of 1 μg/mL. (genetex.com)
Indirect ELISA analysis was performed by coating plate with 50 μL of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135592-pro) at concentrations ranging from 0.0625 μg/mL to 4 μg/mL. (genetex.com)
Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 Nucleocapsid protein (D63G, R203M, D377Y, R385K), His Tag (delta variant) and SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag (wild type) (GTX135592-pro) (21.91-0.34 nM). (genetex.com)

These include messenger ribonucleic acid (mRNA) vaccines, protein subunit and virus-like particle (VLP) vaccines, and non-replicating viral vector vaccines. (canada.ca)
Identification of this gene and expression of recombinant cytotoxin could facilitate the development of improved vaccines against infectious bovine keratoconjunctivitis. (avma.org)
The committee will discuss and make recommendations on the selection of strain(s) to be included in the periodic updated COVID-19 vaccines for the 2023-2024 vaccination campaign. (fda.gov)
and 2) an open forum for collaborative discussions to facilitate advancement of recombinant protein-based COVID-19 vaccines. (fda.gov)
This action includes authorizing the current bivalent vaccines (original and omicron BA.4/BA.5 strains) to be used for all doses administered to individuals 6 months of age and older, including for an additional dose or doses for certain populations. (fda.gov)
Hester MM, Lee CK, Abraham A, Khoshkenar P, Ostroff GR, Levitz SM, Specht CA. Protection of mice against experimental cryptococcosis using glucan particle-based vaccines containing novel recombinant antigens. (umassmed.edu)
To provide insight into the molecular basis of B5 serotype specificity, we solved the crystal structures of recombinant OspC types B and K and compared them to OspC(A). This study represents the first structure of a protective B cell epitope on OspC and will aid in the rational design of OspC-based vaccines and therapeutics for Lyme disease. (cdc.gov)
are modified annually to include the most prevalent strains (usually 2 strains of influenza A and 1 or 2 strains of influenza B). Sometimes slightly different vaccines are used in the northern and southern hemispheres. (msdmanuals.com)
Trivalent vaccines are gradually being superseded by quadrivalent vaccines that cover an additional B virus strain. (msdmanuals.com)

The protein is produced by a recombinant E. coli strain carrying the Bst X DNA Polymerase (exo-) polymerase gene. (qiagen.com)
Compatible with any E. coli strain. (neuromics.com)
The protein is produced by a recombinant E. coli strain carrying the recombinant thermolabile uracil DNA glycosylase gene isolated from a marine bacteria. (qiagen.com)

Those include its use as a critical starting material for transient transfection to produce both viral-vector constructs (e.g., lentivirus or adenoassociated virus) and transient proteins in development (e.g., monoclonal antibody). (bioprocessintl.com)

KBI's approach is the best fit when selecting E. coli as the preferred host for expression of non-glycosylated proteins, including both soluble and insoluble product accumulation including downstream capture, host-cell impurity clearance, and, where necessary, controlled and scalable refolding. (kbibiopharma.com)
Pichia pastoris , a methylotrophic yeast, is our favourite host, which we employ for expression and secretion of recombinant soluble proteins as well as for the biosynthesis of valuable metabolites, e.g. plant terpenoids, through heterologous expression of membrane proteins. (tugraz.at)

A unique method to screen large-scale libraries for industrially useful bacterial strains was recently developed by Tokyo Tech researchers. (titech.ac.jp)
Nonetheless, the traditional method of genetically engineering bacterial strains for high protein production is extremely time consuming. (titech.ac.jp)
The study was led by Associate Professor Tetsuya Kitaguchi from Tokyo Institute of Technology (Tokyo Tech), Japan, and was conducted in collaboration with Ajinomoto Co., Inc. The innovative method, which combines microfluidics and versatile biosensing to quickly identify enhanced bacterial strains that produce the highest amount of a desired protein, is reported in their study published in the journal Small on April 24, 2023. (titech.ac.jp)
Thus, fluorescence intensity is directly related to the amount of the desired protein that each bacterial strain can produce. (titech.ac.jp)
The researchers put their method to the test by screening a huge library of bacterial strains created to produce FGF9, a human cytokine, and subjected to circumstances that cause random mutations. (titech.ac.jp)
Oligonucleotide primers, designed on the basis of amino acid sequences of 2 tryptic peptides derived from 1 such protein and conserved regions of the C and B genes from members of the repeats in the structural toxin (RTX) family of bacterial toxins, were used to amplify cytotoxin-specific genes from M bovis genomic DNA. (avma.org)

The SARS-CoV-2 enters the cells via the human angiotensin-converting enzyme 2 (ACE2) receptor with the use of its spike protein 's receptor-binding domain (RBD). (news-medical.net)
Therefore, we study enzyme characteristics by classical protein analytics and devise novel enzyme assays. (tugraz.at)

Sino Biological, Inc. (SZSE: 301047) has recently deposited reagents for the Omicron variant, including recombinant proteins to Spike and Nucleocapsid, and antibody products to BEI Resources, a central repository for reagents to support infectious disease research. (the-scientist.com)
The coated protein is detected with SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135361) at 1 μg/mL. (genetex.com)
Coated protein was probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (1 μg/mL). (genetex.com)
Several decades ago, it was shown that the OspC-specific monoclonal antibody, B5, was able to passively protect mice from experimental tick-transmitted infection by B. burgdorferi strain B31. (cdc.gov)
Analysis of variable major protein antigenic variation in the relapsing fever spirochete, Borrelia miyamotoi, in response to polyclonal antibody selection pressure. (cdc.gov)
We incubated a low passage parent strain with mouse anti-B. miyamotoi polyclonal antiserum which killed the majority population, however, antibody-resistant reisolates were recovered. (cdc.gov)
Gel electrophoresis protein profiles and immunoblots of the reisolates revealed additional Vlps indicating that new serotype populations were selected by antibody pressure. (cdc.gov)
Osteonectin expression was evaluated using immunohistochemistry with anti-secreted protein acidic and rich in cystein (anti-SPARC) antibody. (bvsalud.org)

Conclusions and Clinical Relevance -A gene was identified in M bovis that encodes a protein with sequence homology to other RTX toxins. (avma.org)
Based on a genetically defined model strain with plasmid based production of the gene for the green fluorescent protein ( GFP ), at first, the optimization of the basic culture parameters pH-value, temperature and dissolved oxygen concentration was performed. (cuvillier.de)
The large number of clinical cases in 2001 selective pressure on the envelope (E) protein gene of may have been due, in part, to a preponderance of infec- some dengue viruses is evident (19), the most extensive tions with dengue 1 viruses. (cdc.gov)

NK cells were detected in all strains except NOD-scid IL2Rgamma(null), and in vitro activated scid, NOD-scid, and NOD-scid beta2m(null) NK cells kill human melanoma lines and primary melanoma cells. (nih.gov)
RNA, in vitro protein production, and cell therapies). (bioprocessintl.com)
Our data suggest that rFg14-3-3e protein can influence various cellular and immunological functions of goat PBMCs in vitro and may be involved in mediating F. gigantica pathogenesis. (biomedcentral.com)
In vitro studies demonstrate that exogenous recombinant Gal3 is internalised by neuronal cell lines and primary neurons where it interacts with endogenous αSyn fibrils. (lu.se)
Cells derived from bone marrow can undergo osteoinduction in vitro in the absence of osteoinductive factors such as bone morphogenetic proteins. (bvsalud.org)

Based on this information, the corresponding recombinant plasmids will be commercially synthesized placing the selected ASFV proteins under the regulation of specific different promoters already tested in their efficacy in our laboratory. (usda.gov)

A pure microbial strain for clean, efficient therapeutic protein production. (kbibiopharma.com)
I am currently expanding my expertise in microbial strain development for protein production, both recombinant and native, as well as the fermentation of filamentous fungi on complex substrates. (lu.se)

rFg14-3-3e protein was expressed in Pichia pastoris . (biomedcentral.com)

Ahead of upstream process development, KBI focuses on microfermentation screening for microbial cell line development (CLD) to yield consistent, high titers across strains and molecules. (kbibiopharma.com)
Upregulation of several target genes has improved recombinant protein titers and yields. (tugraz.at)

The influence of recombinant human interleukins-1 beta and -1 alpha and rat interleukin-1 beta on gastric acid secretion was investigated in awake rats with pylorus ligation. (nih.gov)
The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. (antikoerper-online.de)
i>The chassis of choice for production and secretion of the Histamine Binding Protein is Staphyloccocus Epidermidis, an organism endogenous to the human nasal flora. (igem.org)
Various assays were used to investigate the stimulatory effects of the purified rFg14-3-3e protein on specific functions of goat PBMCs, including cytokine secretion, proliferation, migration, nitric oxide (NO) production, phagocytosis, and apoptotic capabilities. (biomedcentral.com)
We have uncovered several endogenous yeast proteins and mechanisms that render protein secretion or membrane protein expression more efficient and sustainable. (tugraz.at)
We have devised a novel, medium-throughput, random mutagenesis/screening protocol for improved protein secretion in P. pastoris. (tugraz.at)
We have applied multiple approaches to identify key genes that determine protein secretion rates in P. pastoris. (tugraz.at)

Toxoplasma strains in South America are more genetically diverse than those in North America and Europe. (nature.com)
Borrelia miyamotoi is a tick-transmitted spirochete that is genetically grouped with relapsing fever Borrelia and possesses multiple archived pseudogenes that encode variable major proteins (Vmps). (cdc.gov)

The amino acid sequence of the prion protein (PrP) is sion are not well understood. (cdc.gov)
Furthermore, 2 poly- amino acids 108 and 189 are associated with either short morphic regions of the prion protein amino acid sequence (Leu108/Thr189) or long (Phe108/Val189) incubation pe- correlated with profi le differences in these TSE-infected riods in mice ( 7 ). (cdc.gov)
Sequence analysis revealed that rFg14-3-3e protein had 100% identity to 14-3-3 protein from Fasciola hepatica . (biomedcentral.com)

In this study, we observed that the knockout of calcium-dependent protein kinase 3 (CDPK3) in the type II ME49 strain greatly attenuated virulence in mice and significantly reduced cyst formation. (nature.com)
Mice vaccinated with ME49Δ cdpk3 were efficiently protected against the tachyzoites of a variety of wild-type strains, including type I RH, type II ME49, Chinese 1 WH3 and Chinese 1 WH6, as well as the cysts of wild-type strains ME49 and WH6. (nature.com)
Vaccination with Recombinant Cryptococcus Proteins in Glucan Particles Protects Mice against Cryptococcosis in a Manner Dependent upon Mouse Strain and Cryptococcal Species. (umassmed.edu)

Description: Saccharomyces Cerevisiae (strain ATCC 204508 / S288c) Thp1p protein, recombinant protein. (iowaodes.com)

An open reading frame encoding a 927-amino acid protein with a predicted molecular mass of 98.8 kd was amplified from M bovis genomic DNA. (avma.org)

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization). (antikoerper-online.de)
Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. (sigmaaldrich.com)
Qualified cell banks should be used and tested for identity, viability, strain, genotype/phenotype, and presence/copy number of the plasmid vector of interest, along with a description of its structural elements. (bioprocessintl.com)

rFg14-3-3e protein stimulated goat PBMCs to produce interleukin-10 (IL-10) and transforming growth factor beta (TGF-β), corresponding with low levels of IL-4 and interferon gamma (IFN-γ). (biomedcentral.com)

Natural killer (NK) cell function was examined in each mouse strain using standard (51)Chromium release assays. (nih.gov)
5, 6] More recently, affinity-purified antitype VII collagen autoantibodies from epidermolysis bullosa acquisita patients have induced blisters in an adult hairless mouse strain (SKH1), further supporting a pathogenic role of antitype collagen VII autoantibodies. (medscape.com)
Induction of an active model of autoimmunity against type VII collagen in a hairless mouse strain (SKH1) revealed that regulatory T cells, which have been identified for their ability to inhibit the development of autoimmune diseases, do not inhibit the development of autoantibodies against the self-protein mouse-type VII collagen. (medscape.com)

In particular, MST has capabilities to measure ligands/drugs for difficult drug targets such as transcription factors and membrane proteins. (cedars-sinai.edu)
Our particular focus in the yeast lipid field are sterols, their biosynthesis and homeostasis, and their interactions with membrane proteins. (tugraz.at)
We also performed computational assignment of immunodominant epitopes as coordinates of prospective antigenic candidates in most important class of SASPs, the outer membrane proteins (OMPs). (bvsalud.org)

This approach of increasing microbial secretory protein expression has led to breakthroughs in medicine, industry, and agriculture. (titech.ac.jp)
The vector carries the inducible tetracycline promoter/operator for the regulated expression of proteins, the Twin-Strep-tag® for N-terminal and the FLAG-tag for C-terminal fusion to the recombinant protein as well as the Ampicillin Resistance cassette. (neuromics.com)
The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. (antikoerper-online.de)
In response to a brain injury, astrocytes proliferate and become hypertrophic with an increased expression of intermediate filament proteins. (jneurosci.org)
Forced expression of lcn2 or treatment with LCN2 protein increased the sensitivity of astrocytes to cytotoxic stimuli. (jneurosci.org)
In this process, astrocytes proliferate to fill gaps and undergo a typical morphological change: a large cytoplasmic mass, long and branched processes, and increased expression of intermediate filaments such as glial fibrillary acidic protein (GFAP). (jneurosci.org)
We report on the cloning and expression of a recombinant F. gigantica 14-3-3 epsilon protein (rFg14-3-3e), and testing its effects on specific functions of goat peripheral blood mononuclear cells (PBMCs). (biomedcentral.com)
this includes vectors for ligation-independent cloning (LIC), numerous strains of E. coli for expression of challenging constructs and a refrigerated incubator for large-scale production of temperature-sensitive proteins. (cedars-sinai.edu)
What are E. coli Expression Strains? (goldbio.com)
E. coli expression strains, BL21(DE3) and DL39 for example, are used for recombinant RNA/protein exp. (goldbio.com)
We found very high levels of expression of the central complement protein, C3, and complement inhibitor CD59 in human pancreatic islets. (lu.se)
we found that the expression of cartilage protein COMP is associated with metastases and a poor prognosis for patients with various types of solid cancers. (lu.se)

The simple approach combines biosensors and microfluidics to quickly identify mutant strains that secrete large amounts of industrially useful proteins, opening the doors to more applications, like reasonably priced biopharmaceuticals. (titech.ac.jp)
As an alternative, researchers sometimes rely on screening of large-scale libraries for identifying strains which secrete a high amount of protein. (titech.ac.jp)

Recombinant viruses will be then produced using racoon pox vectored virus (RPVV) as templated. (usda.gov)
Phylogenetic studies have shown regular extinction of 1,816 cases of DHF/DSS were reported and dengue 1 also strains of dengue viruses in single locations and emergence was the most frequently isolated serotype. (cdc.gov)

In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. (antikoerper-online.de)
Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. (antikoerper-online.de)

Human PUM3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade. (antikoerper-online.de)

The projects feature state-of-the-art methods for the investigation of cell biology, such as flow cytometry and confocal microscopy, protein interaction analyses using the proximity-ligation assay and Biacore, and genetic manipulation employing the Cas9/CRISPR system. (lu.se)

This protein promotes adhesion of the bacteria to the teeth's surface. (wallstreetpit.com)
We engineer the membranes of microbes, i.e. bacteria and yeasts, regarding lipid and membrane protein composition, either to understand the complex interactions of these hydrophobic compounds or to apply the modified microorganisms for interesting goals of industrial biotechnology. (tugraz.at)
Surface-associated and secretory proteins (SASPs) are attractive targets against bacteria. (bvsalud.org)

Bst X DNA Polymerase is a recombinant, thermostable DNA polymerase from Geobacillus that is homologous to the well-known DNA polymerase from Bacillus stearothermophilus (Bst). (qiagen.com)
Finally, the results from the model strain were successfully transferred to the production of a homologous protein, the levansucrase SacB. (cuvillier.de)

Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. (antikoerper-online.de)

Thus distortion causing the substrate molecule to adopt a strained conformation similar to that of the transition state will lower the energy barrier of the reaction. (wikipedia.org)
A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. (antikoerper-online.de)

Infection by beta coronaviruses (CoVs) in humans causes symptoms ranging from mild, caused by common cold-causing strains, to severe acute respiratory syndrome (SARS), caused by highly virulent strains. (news-medical.net)

The 5-10 most immunogenic African swine fever virus (ASFV) proteins will be selected based in results previously obtained by ARS, PIADC, where these proteins were identified by peptide scanning immune microarray. (usda.gov)

The RBD in the spike protein represents the most dominant target for neutralizing SARS-CoV-2. (news-medical.net)

With modern genetic engineering tools, it is now possible to modify microorganisms so that their production of industrially useful proteins-such as those used in biopharmaceuticals-is enhanced. (titech.ac.jp)
This is because it relies on introducing genetic modifications in individual strains and evaluating the effectiveness of protein production. (titech.ac.jp)
In this case, they were designed to bind to the desired protein, establishing a connection between fluorescence intensity and target protein production. (titech.ac.jp)
Made in Germany - from design to production - by highly experienced protein experts. (antikoerper-online.de)
The aim of this project was the development and targeted optimization of recombinant protein production with Bacillus megaterium. (cuvillier.de)
Subsequently, recombinant protein production was also assessed on the cellular level - using system wide transcriptome and proteome analysis. (cuvillier.de)
Overall, our data suggest a prominent role for GAL3 in the aggregation process of αSYN and LB formation, leading to the production of short species to the detriment of larger strains which triggers neuronal degeneration in a mouse model of PD. (lu.se)

2019) Cartilage Oligomeric Matrix Protein initiates cancer stem cells through activation of Jagged1-Notch3 signaling. (lu.se)

Because investigators typically select one or two immunodeficient mouse strains as recipients, no comprehensive study has been published documenting differences in human tumor engraftment. (nih.gov)
Taking advantage of the increased metastatic potential of RhoC-expressing human (A375) melanoma cells, we evaluate four immunodeficient mouse strains: severe combined immunodeficiency (scid), nonobese diabetic (NOD)-scid, NOD-scid beta2m(null), and NOD-scid IL2Rgamma(null) as xenograft tumor recipients. (nih.gov)
Murine NKG2D recognition of MICA/B is an important receptor-ligand interaction used by NK cells in immunodeficient strains to limit engraftment of human tumors. (nih.gov)
The absolute NK deficiency in NOD-scid IL2Rgamma(null) animals makes this strain an excellent recipient of melanoma and potentially other human malignancies. (nih.gov)
Homology modelling revealed 65% identity between rFg14-3-3e and human 14-3-3 protein YWHAE. (biomedcentral.com)
REFERENCE 1 (bases 1 to 2852) AUTHORS Kuenkel,U. and Schreier,E. TITLE Characterization of recombinant human calicivirus circulating in Germany JOURNAL Unpublished REFERENCE 2 (bases 1 to 2852) AUTHORS Kuenkel,U. and Schreier,E. TITLE Direct Submission JOURNAL Submitted (20-AUG-2002) Molecular Virology, Robert Koch Institute, Nordufer 20, Berlin 13353, Germany FEATURES Location/Qualifiers source 1. (cdc.gov)
Objective: This study evaluated the osteogenic induction of human bone marrow cells by human recombinant bone morphogenetic protein-4 (rhBMP-4) and proteins released by Saos-2 (human osteosarcoma cell line). (bvsalud.org)

OspC is a helical-rich homodimer that interacts with tick salivary proteins, as well as components of the mammalian immune system. (cdc.gov)
Unlocking the Mysteries of the Immune System: new Roles of complement proteins in Diabetes and Cancer. (lu.se)

One of the functions of the FtsZ ring is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. (joplink.net)
However, the involvement of this protein in parasite pathogenesis remains elusive and its effect on the functions of innate immune cells is unknown. (biomedcentral.com)
Because of its involvement in F. gigantica recognition by innate immune cells, rFg14-3-3e protein may have applications for development of diagnostics and therapeutic interventions. (biomedcentral.com)
It is a galactose-binding protein without known catalytic activity and is expressed mainly by activated microglial cells in the central nervous system (CNS). (lu.se)

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. (antikoerper-online.de)
In cases of insoluble protein accumulation in inclusion bodies (IBs), KBI develops high-yielding recovery processes with analytical characterization of conversion to an active product by solubilization and refolding techniques. (kbibiopharma.com)

The concentration of our recombinant proteins is measured using the absorbance at 280nm. (antikoerper-online.de)
Protein concentration is determined by OD 280 absorbance. (qiagen.com)

Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. (joplink.net)
A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. (antikoerper-online.de)
Iron and BIM (Bcl-2-interacting mediator of cell death) proteins were involved in the cytotoxic sensitization process. (jneurosci.org)
LCN2 protein induced upregulation of glial fibrillary acidic protein (GFAP), cell migration, and morphological changes similar to characteristic phenotypic changes termed reactive astrocytosis. (jneurosci.org)
Also, this recombinant protein promoted the release of NO and cell apoptosis, and inhibited the proliferation and migration of goat PBMCs and suppressed monocyte phagocytosis. (biomedcentral.com)
In high cell density cultivations, a high product level, which was significantly higher than that of any other extracellular proteins, produced so far with B. megaterium, could be obtained. (cuvillier.de)
The key feature of this technology is the ability to measure affinity either using a recombinant proteins or proteins expressed in a cell lysate with greater precision. (cedars-sinai.edu)

In addition, you will have the opportunity to learn microbiologic and immunologic techniques and to express and purify recombinant proteins. (lu.se)

A pandemic occurs when an influenza virus emerges that most people do not have immunity from because it is so different from any previous strain in humans. (who.int)

Results -Several proteins ranging in molecular mass from 55 to 75 kd were unique to the hemolytic strain. (avma.org)
The molecular structure of Fasciola gigantica 14-3-3 protein has been characterized. (biomedcentral.com)
In addition to the equipment mentioned above, the molecular therapeutics core facility includes GE MicroCal calorimeters for thermodynamic analysis of protein-ligand interactions through ITC. (cedars-sinai.edu)
We combine structure-based protein engineering and molecular genetics to restrict the activity of the potentially toxic endonuclease to spermatogenesis. (nature.com)

A Waters Breeze 2 HPLC equipped with a number of columns for small molecule and protein chromatography is also available for use. (cedars-sinai.edu)

The above characteristics of Toxoplasma strains pose great challenges to the control of toxoplasmosis. (nature.com)

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Phenomena and Processes83

Disciplines and Occupations3

Anthropology, Education, Sociology and Social Phenomena1

Technology, Industry, Agriculture5

Information Science4

Health Care8

Vaccine17

Escherichia3

Antibodies7

Antigen5

ELISA4

Vaccines9

Coli strain3

Viral1

Soluble2

Bacterial6

Enzyme2

Antibody8

Gene3

Vitro5

Plasmids1

Microbial strain2

Pichia1

Titers2

Secretion7

Genetically2

Sequence3

Mice3

ATCC1

Genomic1

Plasmid3

Interferon1

Mouse strain3

Membrane proteins3

Expression12

Secrete2

Viruses2

Purification2

Insect cells1

Flow Cytometry1

Bacteria3

Homologous2

Baculovirus1

Conformation2

Infection1

Peptide1

Spike1

Production7

20191

Human7

Immune system2

Cells4

Insoluble2

Absorbance2

Cell7

Immunologic1

Humans1

Molecular4

Chromatography1

Characteristics1