• The results presented here clearly demonstrate the differences between the two biovar antiqua lineages and support the notion that grouping Y. pestis strains based strictly on the classical definition of biovars (predicated upon two biochemical assays) does not accurately reflect the phylogenetic relationships within this species. (asm.org)
  • The complete sequence of gyrA gene from 77 strains are reported in this study and analyzed in the context of publicly available sequences from N. meningitidis of other CCs as well as other Neisseria species. (biomedcentral.com)
  • Recombination within N.meningitidis strains and between Neisseria species was identified in SimPlot analysis. (biomedcentral.com)
  • Here, we investigated, in females of both D. melanogaster and D. nigrosparsa , the gene expression between animals uninfected and infected with w Mel, using RNA sequencing to see if the two Drosophila species respond to the infection in the same or different ways. (nature.com)
  • Significant changes shared by the fly species belong to the expression of genes involved in processes such as oxidation-reduction process, iron-ion binding, and voltage-gated potassium-channel activity. (nature.com)
  • The GENOBOX platform will allow industry to determine specific functional benefits of bacterial species in a fast, cheap, and reproducible way, according to NIZO Food Research - who coordinate the consortium behind the platform. (nutraingredients.com)
  • Phylogenetic relationships among 4 marine-biofilm bacterial species identified by 16S rDNA sequences were not predictive of their inductive capacity. (int-res.com)
  • Two bacterial species separated by a genetic distance of 30% shared the greatest capacity for inducing metamorphosis in H. elegans . (int-res.com)
  • The percent of larvae that metamorphosed correlated positively with bacterial density in either natural biofilms or biofilms composed of a single bacterial species. (int-res.com)
  • When results of tests across 4 bacterial species were compared, the most inductive bacterial species was effective in much lower biofilm densities than weakly or non-inductive bacteria. (int-res.com)
  • Dendrograms of intraspecific genetic similarity showed the genetic diversity among the isolated species and bacterial strains. (usp.br)
  • Measure using MALDI-TOF MS. The unique mass spectrum for the bacterial species is rapidly obtained. (shimadzu.com)
  • SuperSpectra TM contains peak groups that can act as markers to offer stable detection of commonly detected bacterial species under multiple conditions. (shimadzu.com)
  • Databases can be supplied even for rarely detected bacterial species. (shimadzu.com)
  • Focusing on the S10 -spc-alpha operon, for which about half the ribosomal proteins are coded, enables genetic information to be associated with molecular weight information from MALDI-TOF MS, even for non-genome sequenced bacterial species. (shimadzu.com)
  • The researchers sequenced a subset of the isolated bacteria and found 28 strains from 12 species with urease genes. (oercommons.org)
  • These bacterial species had not previously been found in the rumen, but this team detected them in metagenomes from 6 ruminant species. (oercommons.org)
  • Recurrence of AR leads an array of alterations in plants that includes inhibited seed germination, growth and productivity, biomass accumulation, photosynthesis, enzyme activities, protein synthesis, gene expression patterns, and over production of active oxygen species (AOS). (degruyter.com)
  • The research team gave 23 volunteers a daily dose of the probiotic strain AH1206 of the bacteria Bifidobacterium longum , one of the most common species of bacteria found in the gut. (mentalfloss.com)
  • They had lower levels of that particular species or species with similar genes. (mentalfloss.com)
  • More than 60 bacterial species have been shown to take up and incorporate DNA (undergo transformation). (gmwatch.org)
  • Background: Poly-3-d-hydroxybutyrate (PHB) that is a promising precursor for bioplastic with similar physical properties as polypropylene, is naturally produced by several bacterial species. (lu.se)
  • A catalogue of 178 reference bacterial birth, and the delivery type is the first core of species has been identified genomes distributed among different factor that has an impact. (who.int)
  • To further investigate the genomic diversity among this group and to help characterize lineages of the plague organism that have no sequenced members, we present here the genomes of two isolates of the "classical" antiqua biovar, strains Antiqua and Nepal516. (asm.org)
  • Within L. monocytogenes , sequences homologous to gtcA were found in all serogroup 4 isolates but not in strains of any other serotypes. (asm.org)
  • The aims of this research work were to determine the patterns of antibiotic resistance in Escherichia coli isolates from the meat of wild or domestically reared pigeons from Spain, to detect the presence of virulence and antibiotic resistance genes, and to carry out a phylogenetic classification of the isolates. (mdpi.com)
  • Genetic diversity of the bacterial isolates was evaluated by rep-PCR. (scielo.br)
  • Gram-positive, spore-forming bacilli comprised most bacterial isolates. (scielo.br)
  • In-vitro Antibacterial Activity of a Fosfomycin-Trimethoprim-Sulfamethoxazole Combination Against a Bacterial Collection of Multidrug-Resistant Clinical Isolates. (asm.org)
  • This study was aimed at determining the urinary bacterial isolates and their susceptibility to selected antibiotics among pregnant women attending antenatal clinics of selected Hospitals in Ilorin, Nigeria. (who.int)
  • Cefoxitin (30g) was used as surrogate to determine phenotypic methicillin resistance in staphylococcus isolates, and the methicillin resistance ( mec A) gene was detected by conventional PCR assay. (who.int)
  • Four of the 9 (44.4%) CoNS isolates were cefoxitin resistant and all the 4 (100%) carried mec A gene. (who.int)
  • Bacterial metabolism selected lignin model compounds (vanillin, vanillate, 4- hydroxybenzoate, p-coumarate, benzoate, and ferulate) was carried out with three of the Pseudomonas isolates and in addition with the strain Pseudomonas putida KT2440 from a Introduction culture collection. (lu.se)
  • Two bacterial strains, R. eutropha JMP222 and Pseudomonas putida KT2442, able to produce chlorocatechols from 3-chlorobenzoate (3-CB) were used. (uai.cl)
  • Genomic information for outlier strains of Pseudomonas aeruginosa is exiguous when compared with classical strains. (frontiersin.org)
  • Based on the results of phylogenetic similarity of 16SrDNA gene sequences, strain DB17 was identified to be Pseudomonas putida. (omicsonline.org)
  • If you have six different strains of Pseudomonas aeruginosa , who wins? (livescience.com)
  • The nptII gene in transgenic potato plants coding for kanamycin resistance, transforms naturally competent cells of the soil bacteria Pseudomonas stutzeri and Acinetobacter BD413 (both harboring a plasmid with an nptII gene containing a small deletion (hence nonfunctional) with the same high efficiency as nptII genes on plasmid DNA ( 3x10-5 -1x10-4) despite the presence of a more than 106 fold excess of plant DNA. (gmwatch.org)
  • A new strain of Salmonella caused outbreaks of severe illness in dairy calves and people, mostly children. (cdc.gov)
  • Initial WVDL testing showed that the calves were carrying a strain of Salmonella serotype Heidelberg bacteria. (cdc.gov)
  • None of them had ever seen a Salmonella strain that caused such a rapid onset of illness or such a high death rate in calves. (cdc.gov)
  • The strain caused severe illness with rates of bloodstream infections and hospitalizations higher than usual for Salmonella . (cdc.gov)
  • Next, Wisconsin veterinarians and public health officials asked CDC to assist with characterizing the Salmonella bacteria strains using a molecular technique known as whole genome sequencing (WGS). (cdc.gov)
  • CDC's PulseNet team examined WGS data from Salmonella Heidelberg strains from Wisconsin and other state laboratories. (cdc.gov)
  • Using WGS, NARMS laboratory scientists took a closer look at the Salmonella bacterial genomes and identified genes typically responsible for antibiotic resistance. (cdc.gov)
  • Histidine requirement in the Salmonella typhimurium strains (Histidine operon). (europa.eu)
  • Spontaneous reversion of the test strains to histidine (Salmonella typhimurium strains) independence is measured routinely in mutagenicity experiments and expressed as the number of spontaneous revertants per plate. (europa.eu)
  • The test chemical did not induce mutation in the Salmonella typhimurium strains both in the presence and absence of S9 metabolic activation system and hence is not likely to be mutagenic under the conditions of this study. (europa.eu)
  • By using a specialist bioinformatics infrastructure and expertise to develop, manage and interpret these data the GENOBOX consortium aims to predict the functional properties of bacterial strains based solely on their genomic sequencing and will compare the data with hundreds of genomes of micro-organisms available in the public domain. (nutraingredients.com)
  • However, this analysis requires accurate reference genomes to identify the specific genes from which RNA reads originate. (osti.gov)
  • Sample-specific meta-genomic assembled genomes (MAGs) were used as reference genomes to accurately identify the origin of RNA reads, and transcript ratios of genes with opposite transcription responses were compared to eliminate biases related to differences in organismal abundance, an approach hereafter named the "diametric ratio" method. (osti.gov)
  • Instead of finding small mutations scattered throughout the DNA, the team found that entirely new segments of DNA had been added to the winning strains' genomes. (livescience.com)
  • Laboratory testing detected infections across multiple states from the same strain and identified a new antibiotic resistance gene. (cdc.gov)
  • Here, we report a new strain with chromosomally integrated ethanologenic pathway under the control of a constitutive promoter, without recombinant plasmids and resistance genes. (biomedcentral.com)
  • Only one position, 91 (83 in E.coli gyrA gene), was linked to CIP resistance. (biomedcentral.com)
  • Thirty-one additional putative resistance markers were identified, as amino acid substitutions were only found in resistant strains. (biomedcentral.com)
  • The evolution of gyrA gene of CC4821 N.meningitidis strains is not dependent on CC4821 evolution or on CIP resistance phenotype. (biomedcentral.com)
  • Analyzing the evolution of gyrA gene within CC4821 strains is critical to monitor the CIP resistance phenotype and the acquisition of new resistance markers. (biomedcentral.com)
  • Virulence genotype analysis revealed that strain CR1 lacked hemolytic phospholipase C and D, three genes for LPS biosynthesis and had reduced antibiotic resistance genes when compared with clinical strains. (frontiersin.org)
  • The competence to acquire multidrug resistance and new virulence factors makes these strains a potential threat. (frontiersin.org)
  • XDR Shigella strains can spread antimicrobial resistance genes to other enteric bacteria. (cdc.gov)
  • data are preliminary and based on broth microdilution susceptibility testing and/or presence of resistance genes and mutations found in whole genome sequences of bacterial DNA. (cdc.gov)
  • Confirmation of Phenotypes of Tester Strains: The phenotypes of the tester strains used in the bacterial reverse mutation assays with regard to membrane permeability (rfa), UV sensitivity (uvrA and uvrB), ampicillin resistance (amp), as well as spontaneous mutation frequencies are checked regularly according to Ames et al. (europa.eu)
  • 24. Yet another alarming signal was China's detection last year, in animal and human samples, of a mechanism of drug resistance, involving the mcr-1 gene, that is easily transferred from one bacterial strain to others, including some with epidemic potential. (who.int)
  • Exploring the Effect of Sub-MIC Antibiotic Exposure on Bacterial Hetero-resistance at Single Cell Level. (asm.org)
  • But now, a new study, published Friday (July 16) in the journal Science Advances , hints that phages may also help their bacterial hosts develop resistance against antibiotic treatments . (livescience.com)
  • Insect-resistant maize line CG00526-176 contains three bacterial genes: the cry1A(b) specific to lepidopterans, the bar gene conferring tolerance to glufosinate, and a bla gene encoding TEM-1 b-lactamase (ampicillin resistance). (gmwatch.org)
  • The researchers investigated the survival of DNA from transgenic maize and the transfer of the antibiotic resistance bla gene to bacteria in the presence of saliva, rumen fluid and silage effluent, which are relevant to horizontal gene transfer in the oral cavity, the rumen, and in silage. (gmwatch.org)
  • Bacterial resistance to antimicrobials has become one of the greatest challenges for clinical microbiologists and healthcare practitioners worldwide. (karger.com)
  • Acquisition of resistance genes has proven to be difficult to characterize and is largely uncontrollable in the environment. (karger.com)
  • Here we sought to characterize conjugal horizontal gene transfer of plasmid-encoded fluoroquinolone resistance genes from two strains of Enterobacteriaceae, one clinical and one from a municipal wastewater treatment plant environment. (karger.com)
  • Escherichia coli strain LR09, containing a plasmid with the aac(6 ′ )-Ib-cr fluoroquinolone resistance gene, did not conjugate with any of the 15 strains tested, while Enterobacter aerogenes strain YS11 conjugated with two strains of E. coli . (karger.com)
  • Hata M, Suzuki M, Matsumoto M, Takahashi M, Sato K, Ibe S, Sakae K: Cloning of a novel gene for quinolone resistance from a transferable plasmid in Shigella flexneri 2b. (karger.com)
  • Antimicrobial resistance occurs through different mechanisms, which include spontaneous (natural) genetic mutations and horizontal transfer of resistant genes through deoxyribonucleic acid (DNA). (who.int)
  • This report presents the status of AMR in Africa by analysing the main types of resistance and the underlying genes where possible. (who.int)
  • We previously engineered an Escherichia coli strain for optimized bioethanol production from lactose-rich wastewater like concentrated whey permeate (CWP), a dairy effluent obtained from whey valorization processes. (biomedcentral.com)
  • Bacterial cancer therapy was developed using probiotic Escherichia coli Nissle 1917 (EcN) for medical intervention of colorectal cancer. (nature.com)
  • Tryptophan requirement in the Escherichia coli strain (Tryptophan operon). (europa.eu)
  • A comparison of four virulent Y. pestis strains with the human-avirulent strain 91001 provides further insight into the genetic basis of virulence to humans. (asm.org)
  • The phylogenetic analysis of CC4821 gyrA gene reveals at least 5 distinct genetic clusters. (biomedcentral.com)
  • The plasmid containing the genetic code for NDM-1 can move from one strain of bacteria to another through a process known as horizontal gene transfer (HGT). (medicalnewstoday.com)
  • The genetic study showed promising results in "silencing" the genes that translate into tau protein, the primary component behind dementia. (medicaldaily.com)
  • The new findings, presented at the annual conference of the Microbiology Society in Edinburgh, Scotland, suggest the bacteria comes in two strains, with subtle differences at the genetic level. (vice.com)
  • They compared the genetic sequence of the winning strains with their ancestors - the versions of those same strains that were first introduced to the pig wounds - to see whether any mutations had cropped up as the bacteria divided in the animals. (livescience.com)
  • They also compared the winning strains' genetic sequences with those of the losing strains. (livescience.com)
  • In two larger clusters, phages infecting different bacterial host genera showed no significant genetic difference. (creationresearch.org)
  • From Texas A&M University, Blanca Lupiani reported that she and her colleagues recently showed that insertion of LTR (long term repeat) sequences into the CVI988 resulted in the generation of CVRM, a virus with similar protective efficacy.The CVRM virus was cloned as a bacterial artificial chromosome, by insertion of mini-F sequences into the US2 gene by homologous recombination. (thepoultrysite.com)
  • The bla gene originates from the cloning vector PUC18 and is not expressed in maize, but has bacterial regulatory sequences that would allow it to become functional were it to be transferred back into bacteria. (gmwatch.org)
  • The paper describes the isolation and characterization of a novel pelagic bacterial strain capable of hepatotoxic microcystin (MC) degradation. (degruyter.com)
  • Detection of a methylcarbamate degradation gene in agricultur al soils using PCR amplification of bacterial community DNA. (cdc.gov)
  • The carbamate insecticide carbofuran (2,3-dihydro-2,2-dimethyl-7-benofurayl-N-methylcarbamate) is biodegraded by a methylcarbamate hydrolase enzyme encoded by a methylcarbamate degradation (mcd) gene cloned from Achromobacter sp. (cdc.gov)
  • Indest KJ, Jung CM, Chen HP, Hancock D, Florizone C, Eltis LD, Crocker FH: Functional characterization of pGKT2, a 182-kb plasmid containing the xplAB genes involved in the degradation of RDX by Gordonia sp. (karger.com)
  • The whole-genome sequenced bacterial strain has applications in enteric disease research and infectious disease research. (atcc.org)
  • We identified eight new regions of genomic plasticity and a plasmid pCR1 with a VirB/D4 complex followed by trimeric auto-transporter that can induce virulence phenotype in the genome of strain CR1. (frontiersin.org)
  • V. cholerae strains have incomplete virulence cassettes particularly due to the absence of genes ctxA e tcpA. (usp.br)
  • The software broadens the range of possible applications using the AXIMA-iD Plus Microorganism Identification System by enabling bacterial characterization down to the strain level. (shimadzu.com)
  • Aim Campylobacter is the leading bacterial pathogen that causes foodborne illnesses worldwide. (usda.gov)
  • AND 3) Positive growth of bacterial pathogen(s) identified from respiratory specimen(s) taken on or after day 3 of ventilation. (who.int)
  • Barrier agains t pathogen s gut bacterial genes in the MetaHIT ation when attempting to extrapolate [10], although Proteobacteria, Ver- catalogue were also well represent- results obtained in mouse models to rucomicrobia, and Fusobacteria are ed in the other metagenomes that the situation in humans. (who.int)
  • Therefore, strains lacking T3SS were thought to be less pathogenic due to the absence of injectosome machinery and its effector toxins (ExoS, ExoT, ExoU, and ExoY) that play a prominent role in acute as well as chronic infections ( Hauser, 2009 ). (frontiersin.org)
  • Although several serotypes of L. monocytogenes are present in the environment and in foods, the majority of infections are caused by strains of serogroup 1/2 (mostly serotypes 1/2a and 1/2b) and 4 (almost always serotype 4b) ( 6 , 35 ). (asm.org)
  • Healthcare professionals use them as a last resort for many bacterial infections, such as those that E. coli and Klebsiella pneumoniae cause. (medicalnewstoday.com)
  • In 2022, about 5% of Shigella infections reported to CDC were caused by XDR strains, compared with 0% in 2015. (cdc.gov)
  • In the United States, the percentage of Shigella infections caused by XDR strains reported to CDC increased from zero in 2015 to 5% in 2022 (Figure). (cdc.gov)
  • Antibiotics can effectively treat E. coli infections outside the digestive tract and most intestinal infections but are not used to treat intestinal infections by one strain of these bacteria. (msdmanuals.com)
  • The CVI988 strain of Marek's disease virus (MDV) is a highly effective vaccine to protect chicken against very virulent strains of MDV. (thepoultrysite.com)
  • The virulent strains of etative cells and activate the spores. (who.int)
  • according to morphological, physiological and its 16S rRNA gene sequence. (researchgate.net)
  • Some of the enzymes which generate aromatic radicals that break down most prominent bacterial strains found were isolated and tax- the complex linkages present in lignin to compounds of lower onomically identified using 16S ribosomal RNA (rRNA) se- molecular weight. (lu.se)
  • Though both strains belong to one of the three classical biovars, they represent separate lineages defined by recent phylogenetic studies. (asm.org)
  • Based on the phylogenetic analysis and structural characteristics, 197 identified bHLH genes could be well classified into 21 groups. (biomedcentral.com)
  • We sequenced and constructed the complete genome of an environmental strain CR1 of P. aeruginosa and performed the comparative genomic analysis. (frontiersin.org)
  • "The new pipe line, as I call it, will allow companies to select the most promising strains for - say - a new flavor, based on genomic sequence data alone, instead of doing a lot of elaborate and expensive experiments," ​explained Wynand Alkema of NIZO. (nutraingredients.com)
  • However, because the functional potential of such micro-organisms are largely determined by its genomic sequence that encodes enzymes and proteins, it is theoretically possible to reveal functionalities from sequencing the genes alone. (nutraingredients.com)
  • Many companies have already sequenced their industrial strains, and now the GENOBOX project is targeting novel ways to translate this genomic data into strain functionality traits such as GI-survival, yield, probiotic properties, safety, or production of flavour compounds. (nutraingredients.com)
  • Herein, a targeted discovery method for natural macrolactams was devised by coupling genomic signature-based PCR screening of a bacterial DNA library with spectroscopic signature-based early identification of macrolactams. (bvsalud.org)
  • And in fact, the phages in question first entered the pig wounds on the DNA of the losing bacteria strains. (livescience.com)
  • But there are specific regulatory elements in other strains that give those strains different metabolic capabilities. (frontiersin.org)
  • Test chemical did not induce mutation in mammalian cell line in the presence and absence of metabolic activation and hence it is not likely to classify as a gene mutant in vitro. (europa.eu)
  • The new strains contained unique genes compared to known related strains, indicating new metabolic functions. (oercommons.org)
  • The NDM-1 gene allows the bacterium to produce an enzyme that neutralizes the activity of these antibiotics. (medicalnewstoday.com)
  • A bacterium carrying the NDM-1 gene is considered a very powerful superbug. (medicalnewstoday.com)
  • Under the conditions of this study, the test material had no mutagenic activity in the applied bacterium tester strains. (europa.eu)
  • An effective profenofos and λ-cyhalothrin degrading bacterium (strain DB17) was isolated from soil samples collected from Qassim region, Saudi Arabia. (omicsonline.org)
  • Strain ZGt-1, an Antibacterial Peptide-Producing Bacterium from Hot Springs in Jordan. (lu.se)
  • They have since learned that horizontal gene transfer (HGT) between bacteria that express KPC and NDM-1 is possible . (medicalnewstoday.com)
  • The evidence for horizontal gene transfer is accumulating. (gmwatch.org)
  • E. coli strain DH5a was the test micro-organism for horizontal gene transfer. (gmwatch.org)
  • In summary, horizontal gene transfer can occur before the DNA is completely broken down, even when the breakdown is rapid, as in the rumen or in silage. (gmwatch.org)
  • DNA breakdown is extremely slow in saliva, and hence the oral cavity will be a very important site for horizontal gene transfer. (gmwatch.org)
  • The tfd I CDEF gene cluster was inserted in the chromosome of these strains using miniTn5-derived vectors that allow expression of the Tfd enzymes driven by the lacI q /P trc or tfdR/P tfd-I regulatory systems, and therefore, responding to the inducers isopropyl-ß-Dthiogalactopyranoside (IPTG) or 3-CB, respectively. (uai.cl)
  • These strains have apparent differences in several phenotypic characteristics ( Yang and Yang, 2017 ). (frontiersin.org)
  • LT and ST toxin-producing genes), EPEC strains positive in PCR for LT, ST or both (eae gene), and EAEC (pCVD432 plasmid) were considered ETEC. (who.int)
  • A bacterial strain that carries NDM-1 will be resistant to even some of the strongest antibiotics. (medicalnewstoday.com)
  • The gene makes bacteria resistant to antibiotics. (medicalnewstoday.com)
  • CDC defines XDR Shigella bacteria as strains that are resistant to all commonly recommended empiric and alternative antibiotics - azithromycin, ciprofloxacin, ceftriaxone, trimethoprim-sulfamethoxazole (TMP-SMX), and ampicillin. (cdc.gov)
  • Viruses that infect bacteria may drive the evolution of drug-resistant superbugs by inserting their genes into the bacterial DNA, a new study suggests. (livescience.com)
  • The isolation of bacterial pathogens resistant to the commonly prescribed antibiotics from pregnant women symptomatic and asymptomatic for UTI calls for early screening of all pregnant women for UTI during antenatal care service delivery. (who.int)
  • These two "winning" strains produced small, wrinkled-looking colonies of bacteria that congregated into biofilms - clusters of bacterial cells that secrete a slimy substance that offers them protection from both the host immune system and attacks by phages. (livescience.com)
  • Some phage clusters infected only one family of bacterial hosts, whereas others were more promiscuous. (creationresearch.org)
  • Several smaller phage clusters were found, which were already described in the scientific literature, thereby adding candidate bacteriophage strains to these groups. (creationresearch.org)
  • Public health, animal health, environmental, and agricultural experts worked together to track the spread of this strain and teach about infection prevention. (cdc.gov)
  • The WHO suggest that a woman who is pregnant, for example, could develop a kidney infection that transfers into the bloodstream with a strain containing NDM-1. (medicalnewstoday.com)
  • Shigellosis is an acute enteric infection that is an important cause of domestically acquired and travel-associated bacterial diarrhea in the United States. (cdc.gov)
  • Mice homozygous for a knock-out allele exhibit impaired selective autophagy and abnormal response to bacterial infection in MEFs. (jax.org)
  • In particular, the bacterial infection often affects people with compromised immune systems , whether due to conditions like cystic fibrosis or drugs that suppress the immune system, like steroids. (livescience.com)
  • Knowing that P. aeruginosa can be so difficult to kill, the team wondered how different strains of the microbe stack up against each other, and what makes the superior strains so good at triggering hard-to-treat infection. (livescience.com)
  • that normally reside in the intestine of healthy people, but some strains can cause infection in the digestive tract, urinary tract, or many other parts of the body. (msdmanuals.com)
  • However, some strains of E. coli have acquired genes that enable them to cause infection. (msdmanuals.com)
  • The cause is sometimes a bacterial infection. (msdmanuals.com)
  • The recent success gut microbial genes, obtained after has fantastic potential, it has only of faecal microbiota transplantation, sequencing whole faecal microbiota been about 10 years since the sci- especially in the context of Clostridi- metagenomes from 124 European indi- entific community first realized its im- um difficile infection, argues for such viduals [4]. (who.int)
  • Which bacterial strains, vectors and genes are needed. (igem.org)
  • The two variants exhibit different capability to form biofilms and to express genes involved in polysaccharide biosynthesis, flagellar synthesis, and the AI-2 quorum-sensing system [ 18 ]. (hindawi.com)
  • Later on, CC4821 became also a dominant lineage among serogroup B strains since the first report in 2005. (biomedcentral.com)
  • TA of strains of serogroup 1/2 and 3 consists of polyribitol phosphate, with N -acetylglucosamine (Glc-NAc) and rhamnose (in the case of serogroup 1/2) substituents on ribitol (Fig. 1 ). (asm.org)
  • Heterologous expression of the pathway genes from Cupriavidus necator has enabled PHB production in the yeast Saccharomyces cerevisiae from glucose as well as from xylose, after introduction of the fungal xylose utilization pathway from Scheffersomyces stipitis including xylose reductase (XR) and xylitol dehydrogenase (XDH). (lu.se)
  • This gene cluster allows dechlorination and channelling of chlorocatechols into the intermediate metabolism. (uai.cl)
  • As a single colony can be analyzed, colonies derived from multiple bacterial strains on the agar medium do not hinder analysis. (shimadzu.com)
  • Gut bacterial colonies tend to be very stable even in the face of probiotic treatments, and studies have indicated that alterations in the gut bacteria in cases of fecal transplants tend to be temporary. (mentalfloss.com)
  • As was conducted using traditional microbio- many colonies as required for finding the logical methods and with commercial anti- isolate carrying these particular genes were sera if necessary. (who.int)
  • The strain showed extreme stability in 1-month subculturing, with CWP fermentation performances similar to the ethanologenic plasmid-bearing strain. (biomedcentral.com)
  • Furthermore, the strains that are sharing the sequence of 4 or more genes (identified as a number for convenience) among the 7 genes used to determine STs could be classified into the same clonal complex (CC) [ 3 ]. (biomedcentral.com)
  • Chloroaromatic pollutants from bleached Kraft pulp mill effluents (BKME) are difficult to degrade, because bacterial strains present in BKME aerobic treatments, only partially degrade these compounds, accumulating the corresponding chlorocatechol intermediates. (uai.cl)
  • Experiments with BKME aerobic treatment microcosms showed that the catabolic performance of the engineered bacteria was also lower than the wildtype R. eutropha strain JMP134. (uai.cl)
  • An aerobic bacterial strain C-14-1 was isolated from an acrylic fiber wastewater. (researchgate.net)
  • The team tackled this question by introducing six different strains of P. aeruginosa into burn wounds on pigs. (livescience.com)
  • The two winning strains of P. aeruginosa did not immediately produce biofilms upon entering the pigs, but instead entered this protectively slimy state as time progressed. (livescience.com)
  • Blue squares indicate phenotypes directly attributed to mutations/alleles of this gene. (jax.org)
  • The objectives were to evaluate the effects of Corynebacterium glutamicum cell mass (CGCM) supplementation, replacing blood plasma in diets, on the gene expressions of biomarkers associated with peptidoglycan and their correlation with intestinal health in nursery pigs. (usda.gov)
  • Staphylococcus , Corynebacterium , by dietary habits as well as by the ed, the number of frequent genes and Propionibacterium [8]. (who.int)
  • In serotype 4b, gtcA appears to be the first member of a bicistronic operon which includes a gene with homology to Bacillus subtilis rpmE , encoding ribosomal protein L31. (asm.org)
  • We found 453 single nucleotide polymorphisms in protein-coding regions, which were used to assess the evolutionary relationships of these Y. pestis strains. (asm.org)
  • Crescentin is a protein which is a bacterial relative of the intermediate filaments found in eukaryotic cells. (wikipedia.org)
  • Transcription factors (TFs) are protein molecules with special structure and function of regulating gene expression, which plays many crucial roles in plant growth and development [ 1 ]. (biomedcentral.com)
  • This indicates that the rpoX gene is involved in biofilm formation and stress response but the effects are controlled by colony phase variation in V. alginolyticus . (hindawi.com)
  • In this case, the winning strains showed "hyperbiofilm formation," far beyond any biofilm formation observed in the competing strains. (livescience.com)
  • Procedure for Growing Cultures: The frozen bacterial cultures were thawed at room temperature and 200 μL inoculum were used to inoculate each 50 mL of Nutrient Broth No.2 for the overnight cultures in the assay. (europa.eu)
  • As part of a program to explore bacterial metabolites with immunomodulatory potential, S. pyogenes metabolites were assayed in a cell-based immune assay, and a single membrane lipid, 18:1/18:0/18:1/18:0 cardiolipin, was identified. (bvsalud.org)
  • Serotype 4b strains are unique in bearing both galactose and glucose substituents on the Glc-NAc of TA (Fig. 1 ). (asm.org)
  • PCR) of mixed culture was used for the characterized biochemically according to detection of diarrheogenic E. coli strains. (who.int)
  • There has been much research on AmtR structure, functions, and regulons in the type strain C. glutamicum ATCC 13032, but little research in other C. glutamicum strains. (frontiersin.org)
  • The RS_01910, RS_01915, RS_15995, and RS_16000 are not present in the type strain C. glutamicum ATCC 13032. (frontiersin.org)
  • The research on transcription factors in C. glutamicum was mainly focused on the type strain C. glutamicum ATCC 13032. (frontiersin.org)
  • Dangshansuli') has already been reported, there is still a lack of clarity regarding the bHLH family genes and their evolutionary history. (biomedcentral.com)
  • However, a new study finds that selecting the right bacterial strain for the gut environment can make a major difference in whether or not the probiotic can impact the microbiome. (mentalfloss.com)
  • But if someone has lost a particular strain of bacteria or their body never had it in the first place, it's possible to repopulate the microbiome with that strain. (mentalfloss.com)
  • Preliminary studies can be carried out in bacterial expression systems, using the strong T5 promoter, which is recognized by E. coli polymerase, and allows efficient expression of proteins in any E. coli strain. (qiagen.com)
  • Inserts encoding proteins of interest are cloned into appropriate constructs and transformed into a suitable E. coli strain for expression. (qiagen.com)
  • A total of 2164 orthologous genes were used. (nature.com)
  • In this study, 159 bacteriophage strains which had 200 or more orthologous proteins in their genome were examined using the Gene Content Method. (creationresearch.org)
  • Microbial communities interact at the molecular level with their biotic and abiotic surroundings, forming integral parts of geochemical cycles (e.g. nitrogen, carbon, sulphur and water) and even helping to form the infrastructure that holds that environment in place e.g. the exoproteins formed by the bacterial community in soils hold the soil particles together. (biomedcentral.com)
  • A 0.4-kbp BamHI-KpnI fragment of the mcd gene was used as a DNA probe to monitor soil microbial populations capable of degrading carbofuran in soils from twelve contrasting agricultur al sites, representing seven soil series from five U.S. states. (cdc.gov)
  • Soil bacterial community DNA was extracted and humic acid contaminants removed prior to PCR amplification of mcd. (cdc.gov)
  • Results: In this study the acetoacetyl-CoA reductase gene from C. necator (CnAAR), a NADPH-dependent enzyme, was replaced by the NADH-dependent AAR gene from Allochromatium vinosum (AvAAR) in recombinant xylose-utilizing S. cerevisiae and PHB production was compared. (lu.se)
  • Strain does not induce hardening of the structure, unlike eukaryotic IFs that do. (wikipedia.org)
  • Analytical method was developed and validated for the determination of profenofos and λ-cyhalothrin in bacterial medium to monitor the intended biodegradation of both compounds. (omicsonline.org)
  • Four new chlorinated cycloaromatized enediyne compounds, jejucarbosides B-E (1-4), were discovered together with previously-identified jejucarboside A from a marine actinomycete strain. (bvsalud.org)
  • Many strains that appeared to be different using PFGE testing were found to be genetically similar when compared using WGS, indicating that the strains were all related. (cdc.gov)
  • Genes belonging to proteases, bacterial exporters and DNA stabilization were found to be under strong positive selection, thus facilitating pathogenicity and survival of the outliers. (frontiersin.org)
  • The Wolbachia strain w Mel was recently found to increase locomotor activities and possibly trigger cytoplasmic incompatibility in the transinfected fly Drosophila nigrosparsa . (nature.com)
  • We analyzed twenty fecal samples taken from four premature infants (4-6 time points from each infant), and found significantly higher diametric ratios of genes associated with low oxygen levels in samples of infants later diagnosed with NEC than in samples without NEC. (osti.gov)
  • The gene for this is widespread in bacteria found in India and Pakistan, especially in hospitals. (medicalnewstoday.com)
  • The strain was found to belong toNocardia sp. (researchgate.net)
  • Catechol 2,3-dioxygenase (C23O) gene was found andamplified with the designed primers from the total DNA of C-14-1. (researchgate.net)
  • The P. acnes strain found on people without acne has genes that scientists believe help prevent bacteria from colonizing the skin. (vice.com)
  • An association was found between experience of caries in the children and the presence of bacteria carrying the spaP+ gene. (bvsalud.org)
  • Different gene expression patterns, shown by tools such as quantitative PCR, microarrays, and high-throughput RNA sequencing, reveal how organisms respond to different environments. (nature.com)
  • RNA sequencing is a powerful method to study differential gene expression because it can detect a whole gene expression across particular tissues 1 . (nature.com)
  • The intrinsic limitation of P BAD for the gene expression is known to be negated by glucose and afflicted with all-or-nothing induction in host bacteria. (nature.com)
  • As a result, the reprogrammed strain (designated EcNe) enabled efficient expression of HlyE in a temporal control manner. (nature.com)
  • The objective of the work presented in Chicago was to evaluate CVMR-BAC as an expression vector of foreign genes. (thepoultrysite.com)
  • The objective of work presented by Sanjay M. Reddy of Texas A&M University was to evaluate potential cloning sites in the HVT genome for stable expression of foreign genes. (thepoultrysite.com)
  • GXD's primary emphasis is on endogenous gene expression during development. (jax.org)
  • Spontaneous Reversion of Tester Strains: Each test strain reverts spontaneously at a frequency that is characteristic of the strain. (europa.eu)
  • After mixing the test material solution, the phosphate buffer (pH 7.4) or the S9 mix and the culture of the test strain in a test tube, the mixture was incubated at 37°C for 20 minutes. (europa.eu)
  • Cancer gene therapy approaches include the direct killing of tumor cells by injecting a therapeutic gene into the tumor cell or employing vaccine strategies to deliver an immunomodulatory gene that stimulates the immune system to recognize tumor antigens [ 1 ]. (biomedcentral.com)
  • Abstract Starting from mature vegetable compost, four bac- the utilization of these bacteria as cell factories for upgrading terial strains were selected using a lignin-rich medium. (lu.se)
  • Ampicillin or TMP-SMX are recommended as alternative treatments for susceptible strains [2]. (cdc.gov)
  • The genotypic profiles obtained via ERIC-PCR will be used to construct a database which, through the identification/matching of strains implicated in outbreaks, could be used in the Epidemiologic Vigilance of gastroenteritis. (usp.br)