AnatomyOrganismsChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesInformation Science
Electric StimulationPhosphorylationSerineTyrosineEnzyme ActivationPhosphoproteinsProtein-Serine-Threonine KinasesCell LineProtein KinasesCells, CulturedMolecular Sequence DataAmino Acid SequenceOxidative PhosphorylationPhosphoserineProtein-Tyrosine KinasesProtein Kinase CThreonineCyclic AMP-Dependent Protein KinasesElectric Stimulation TherapyEnzyme InhibitorsKineticsSignal TransductionStimulation, ChemicalProtein BindingPhosphotyrosineCalciumPhosphopeptidesProto-Oncogene Proteins c-aktMitogen-Activated Protein KinasesBlotting, WesternTransfectionTime FactorsMutationPhysical StimulationDeep Brain StimulationPhosphothreonineRecombinant Proteinssrc-Family KinasesProto-Oncogene ProteinsProtein Processing, Post-TranslationalRats, Sprague-DawleyPhosphatidylinositol 3-KinasesMitogen-Activated Protein Kinase 1Peptide MappingBinding SitesIntracellular Signaling Peptides and ProteinsCyclic AMPCalcium-Calmodulin-Dependent Protein KinasesCasein Kinase IIPhosphoprotein PhosphatasesMitogen-Activated Protein Kinase 3DNA-Binding ProteinsTetradecanoylphorbol AcetateDose-Response Relationship, DrugProtein Structure, TertiaryCell Line, TumorTranscranial Magnetic StimulationImmunoblottingAdenosine TriphosphateMAP Kinase Signaling SystemExtracellular Signal-Regulated MAP KinasesGlycogen Synthase Kinase 3Precipitin TestsModels, BiologicalGene Expression RegulationProteinsMutagenesis, Site-DirectedAdaptor Proteins, Signal TransducingImmunoprecipitationRecombinant Fusion ProteinsHeLa Cellsp38 Mitogen-Activated Protein KinasesCOS CellsCarrier ProteinsRNA, MessengerElectrophoresis, Polyacrylamide GelInsulinTumor Cells, CulturedProtein TransportCell MembraneMembrane ProteinsApoptosisCell NucleusIsoenzymesCell Cycle ProteinsBase SequenceTranscription FactorsOkadaic Acid3T3 CellsMyosin Light ChainsMice, Inbred C57BLSubstrate SpecificityRabbitsProtein Kinase InhibitorsNeuronsTranscription, GeneticFibroblastsCell DivisionProtein Tyrosine PhosphatasesCattle
Tyrosine phosphorylationProteinDopaminergic stimulationERK1Kinase activityPI3KGlucose uptakeRegulatesLong-term potentiPathwayMyosinStriatalResiduesAMPKInhibitorULK1SMAD2IsoformMiceIntracellularRegulationDephosphorylationAMPAThrombinMechanismsCellsAdenosinePhosphatidylinositolReceptorInsulinIncreaseDecreaseActivitySignificantlyModulateEffectMobilizationExpressionActivationResponseRegulatoryMolecularFoundEffectsStateValuesGroup
Tyrosine phosphorylation12
Protein12
  • Glutamate inputs activate Cdk5, which inhibits postsynaptic dopamine D1 receptor (D1R)-mediated signaling by phosphorylating DARPP-32 at Thr75 (Thr75-DARPP-32) in the striatum, since DARPP-32 with Thr75 phosphorylation (DARPP-32-pThr75) functions as an inhibitor of cAMP-dependent protein kinase A (PKA). (frontiersin.org)
  • Here, we used time-resolved high-throughput proteomic analyses to identify and quantify the phosphorylation events and protein-protein interactions encoding T cell ligand discrimination in antigen-experienced T cells. (nature.com)
  • We defined quantitative signatures of ligand affinity based on protein phosphorylation and protein-protein interaction (PPI) stoichiometry for critical molecular events associated with TCR signaling. (nature.com)
  • cAMP-mediated stimulation of tyrosine hydroxylase mRNA translation is mediated by polypyrimidine-rich sequences within its 3'-untranslated region and poly(C)-binding protein 2. (rochester.edu)
  • Protein kinase C (PKC) activator TPA also induced the phosphorylation of p70 S6K. (nih.gov)
  • The effect of phosphorylation by cyclic GMP-dependent protein kinase (G-kinase) on the activity of the plasmalemmal Ca2+-transport ATPase was studied on isolated plasma membranes and on the ATPase purified from pig erythrocytes and from the smooth muscle of pig stomach and pig aorta. (portlandpress.com)
  • Evidence is presented showing that the apparent phosphorylation occurs in a contaminant protein, possibly myosin light-chain kinase. (portlandpress.com)
  • Rho-kinase regulates a second pathway that involves activation of LIM-kinases (LIMKs) and subsequent phosphorylation and inactivation of cofilin, an actin dynamizing protein. (uni-muenchen.de)
  • Using gene knockout, transgenic and molecular approaches we are investigating the signal mechanisms underlying regulation of protein phosphorylation and sperm function. (kent.edu)
  • Snow AJ, Puri P, Acker-Palmer A, Bouwmeester T, Vijayaraghavan S, Kline D. Phosphorylation-dependent interaction of tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein (YWHA) with PADI6 following oocyte maturation in mice.Biol Reprod. (kent.edu)
  • Transfection of cells in culture with ASO targeting PTEN reduced PTEN mRNA and protein levels and increased insulin-stimulated Akt phosphorylation in α-mouse liver-12 (AML12) cells. (diabetesjournals.org)
  • We demonstrate that DARPP-32 inhibits MCF-7 cell migration and that this effect requires phosphorylation of Threonine-34, an event catalyzed by protein kinase A (PKA) and strongly induced by detachment of cells from the culture substrate. (lu.se)
Dopaminergic stimulation1
  • We here report that Cdk5 with Tyr15 phosphorylation (Cdk5-pTyr15) is enriched in the mouse striatum, where dopaminergic stimulation inhibited phosphorylation of Tyr15-Cdk5 by acting through the D2 class dopamine receptors. (frontiersin.org)
ERK13
  • Based on a previous report, we studied the potential involvement of the adenosine A1 receptor in the effect of CBC on these cells and found that the selective adenosine A1 receptor antagonist, DPCPX, counteracted both ERK1/2 phosphorylation and up-regulation of nestin by CBC, indicating that also adenosine is involved in these effects of CBC, but possibly not in CBC inhibitory effect on GFAP expression. (nih.gov)
  • Functional studies revealed that WSG reduced phosphorylation of ERK1/2 in cells upon either EGF or TGFβ stimulation. (greenmedinfo.com)
  • Isoproterenol-stimulated phosphorylation of ERK1/2 was impaired in SHR. (aspetjournals.org)
Kinase activity1
  • One of the regulatory mechanisms that modulate the Cdk5 kinase activity is phosphorylation of conserved residues ( Dhavan and Tsai, 2001 ). (frontiersin.org)
PI3K4
Glucose uptake1
Regulates2
  • Here, we show that in the mouse striatum, dopamine receptor stimulation negatively regulates phosphorylation of Tyr15-Cdk5 most likely through a D2R-mediated mechanism. (frontiersin.org)
  • The phosphorylation of MLC primarily regulates the force production and maintenance in the smooth muscle [ 6 ]. (hindawi.com)
Long-term potenti2
  • Subthreshold stimulation induced spinal long-term potentiation in GluA1 phosphomimetic mutant mice, but not in wild-type mice. (jneurosci.org)
  • Another effect of GSK3-beta over-expression was the impeded transport of synaptically active a-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors to the cell surface after glycine stimulation, indicating negative effects on long-term potentiation and cognition. (europa.eu)
Pathway2
  • The signaling pathway Rho-kinase/LIMKs/cofilin phosphorylation during platelet activation and the question, how the phosphorylation of cofilin affects the actin dynamics underlying platelet activation, has not previously been studied. (uni-muenchen.de)
  • item [-] A distinct pathway for Erk phosphorylation is activated, and those cells are anergized for IgE, but not fMLP stim %note here: maybe we want to do pErk/ 63 staining with fMLP stim \item [-] while peroxide effect is thought to be secondary to PTPs, neither lyn, nor syk are activated %should we try to look at fyn? (openwetware.org)
Myosin2
Striatal2
  • attenuates abnormally increased striatal phosphorylation of both Tyr15-Cdk5 and Thr75-DARPP-32, as does L-3,4-dihydroxyphenylalanine (L-dopa). (frontiersin.org)
  • Using the 6-hydroxydopamine lesion model of Parkinson's disease and differential display PCR, we have identified a set of more than 30 genes whose expression rapidly increases in response to stimulation of striatal dopamine D 1 receptors. (jneurosci.org)
Residues2
  • This is consistent with reports that the slow phosphorylation of tyrosine residues of the Lat adaptor associates with recruitment and activation of the phospholipase Plcγ1, thereby constituting an important kinetic bottleneck for ligand discrimination 11 . (nature.com)
  • While the phosphorylation of tyrosine residues in the immunoreceptor tyrosine-based activation motifs (ITAMs) of Cd3 and the inducible interaction between Cd3 and Zap70 remained largely unaffected, the abundance of activated Zap70 dropped with decreasing peptide affinity. (nature.com)
AMPK1
  • During starvation AMPK promotes autophagy by activating ULK1 via phosphorylation at Ser317, Ser555, and Ser777, whereas in the presence of nutrients mTORC1 suppresses autophagy by hampering interaction between AMPK and ULK1 through phosphorylation of ULK1 at Ser757 [ 7 ]. (springer.com)
Inhibitor2
  • Moreover, in the 1-methyl-4-phenyl-1,2,4,6-tetrahydropyridine (MPTP) mouse model, dopamine deficiency caused increased phosphorylation of both Tyr15-Cdk5 and Thr75-DARPP-32 in the striatum, which could be attenuated by administration of L-3,4-dihydroxyphenylalanine and imatinib (STI-571), a selective c-Abl inhibitor. (frontiersin.org)
  • Both the PI-3K inhibitor wortmannin and PKC inhibitor calphostin C blocked the phosphorylation of p70 S6K mediated by the growth factors. (nih.gov)
ULK11
  • constitutively energetic (ca)RhoA repressed mTORC1 signaling as evaluated by phosphorylation of p70S6K1 (Thr389), 4E-BP1 (Ser65) and ULK1 (Ser757). (nos-nop.org)
SMAD22
  • Reduced SMO-dependent expression of Gli1 was demonstrated in both Tsc1 −/− and Tsc2 −/− cells, and we found that Tsc1 is required for TGF-β induced phosphorylation of SMAD2/3 and subsequent expression of the HH signaling effector and transcription factor GLI2. (springer.com)
  • Furthermore, we show expression of MSTN by CML mesenchymal stromal cells, and that myostatin propeptide has a direct and instant effect on CML cells, independent of myostatin, by demonstrating binding of myostatin propeptide to the cell surface and increased phosphorylation of STAT5 and SMAD2/3. (haematologica.org)
Isoform2
  • Each isoform-specific antibody recognizes endogenous levels of the specified Akt isoform independent of its phosphorylation state. (cellsignal.com)
  • Differential phosphorylation and isoform expression of tau are believed to regulate the assembly and stabilisation of microtubuli. (europa.eu)
Mice1
Intracellular1
Regulation1
  • One of the important aspects of platelet activation is to understand the dynamic regulation and rearrangement of the cytoskeleton after stimulation. (uni-muenchen.de)
Dephosphorylation2
  • EA caused a significant increase in the MLC dephosphorylation and MLCK phosphorylation levels in the group compared to the sham EA and model groups. (hindawi.com)
  • It is suggested that the phosphorylation of MLC catalyzed by Ca 2+ -calmodulin- (CAM-) dependent MLCK and dephosphorylation catalyzed by MLCP help regulate the contraction and relaxation of smooth muscle [ 8 , 9 ]. (hindawi.com)
AMPA3
  • Here we showed that social defeat stress enhanced plantar incision-induced AMPA receptor GluA1 phosphorylation at the Ser831 site in the spinal cord and greatly prolonged plantar incision-induced pain. (jneurosci.org)
  • In addition, stress hormones enhanced GluA1 phosphorylation and AMPA receptor-mediated electrical activity in the spinal cord. (jneurosci.org)
  • Therefore, spinal AMPA receptor phosphorylation contributes to the mechanisms underlying stress-induced pain transition. (jneurosci.org)
Thrombin2
  • Surprisingly, the activation of LIMK-1 failed to increase cofilin phosphorylation during shape change induced by LPA as well as by thrombin. (uni-muenchen.de)
  • After stimulation with LPA and thrombin under conditions, where platelet aggregation could not occur, the kinetics of cofilin de- and rephosphorylation were unperturbed indicating their independence of integrin IIbβ3 engagement. (uni-muenchen.de)
Mechanisms1
Cells6
  • In this study we have investigated the involvement of PI-3K and its downstream target p70 S6K in the signaling response of corneal epithelial cells after HGF and KGF stimulation. (nih.gov)
  • These studies suggest that PI-3K and p70 S6K are important signal transducers in the stimulation of corneal epithelial cells by HGF and KGF. (nih.gov)
  • Alterations of histone phosphorylation in rat spleen cells after treatment with the aromatic amine, 4,4'-methylene-bis(2-chloroaniline). (cdc.gov)
  • A study was conducted on the effects of 4,4'-methylene-bis-(2- chloroaniline) (95534) (MOCA) on histone phosphorylation in rodent spleen cells. (cdc.gov)
  • It is proposed that G-kinase stimulates the plasmalemmal Ca2+ pump of smooth-muscle cells indirectly via the phosphorylation of an associated PI kinase. (portlandpress.com)
  • The experiments consist of kinetic (time- kinetic data is typically not sufficient to constrain the course) measurements after TGF-b stimulation under differ- parameter values to a single optimal solution, and multiple ent conditions: untreated cells and three cases in which parameter sets can explain the available data. (lu.se)
Adenosine1
Phosphatidylinositol2
  • The stimulation of the (Ca2+ + Mg2+)-ATPase activity by G-kinase was only observed in the presence of phosphatidylinositol (PI). (portlandpress.com)
  • G-kinase, but not A-kinase, stimulated the phosphorylation of PI to phosphatidylinositol phosphate (PIP) in a preparation of (Ca2+ + Mg2+)-ATPase obtained by calmodulin affinity chromatography from smooth muscle, but not in a similar preparation from erythrocytes. (portlandpress.com)
Receptor5
  • In 2002, it was discovered by Hilmar Bading and co-workers that the cellular consequences of NMDA receptor stimulation depend on the receptor's location on the neuronal cell surface. (wikipedia.org)
  • Although low-affinity ligands induced phosphorylation of the Cd3 chains of the T cell receptor and the interaction of Cd3 with the Zap70 kinase as strongly as high-affinity ligands, they failed to activate Zap70 to the same extent. (nature.com)
  • In this study, we examined whether H1R desensitization contributes to regulating the decay rate of TRPC6 activity upon receptor stimulation. (mdpi.com)
  • Binding of insulin to its receptor activates the insulin receptor tyrosine kinase, resulting in autophosphorylation and phosphorylation of several substrates, including insulin receptor substrate (IRS)-1 through -4. (diabetesjournals.org)
  • Mutated or overexpressed ALK receptor tyrosine kinase no longer requires stimulation from outside the cell to be phosphorylated. (medlineplus.gov)
Insulin1
  • The RhoAGTP mediated repression of mTORC1 signaling occurred independent of leucine or insulin induced stimulation. (nos-nop.org)
Increase3
  • HGF induced three- to five-fold increase in PI-3K activity in 5-10 min, whereas KGF stimulation resulted in two- to three-fold increase in activity in 2-10 min. (nih.gov)
  • A significant increase in histone phosphorylation was seen following exposure to 10microM MOCA. (cdc.gov)
  • Not only GSK3-beta, but also its isoenzyme GSK3-alpha were shown to increase tau phosphorylation. (europa.eu)
Decrease1
  • MOCA incubation resulted in an initial transient decrease in histone phosphorylation followed by stimulation. (cdc.gov)
Activity2
  • Both growth factors also caused the phosphorylation of p70 S6K and stimulation of its activity. (nih.gov)
  • The stimulation of the (Ca2+ + Mg2+)-dependent ATPase activity of the purified Ca2+ pump reconstituted in liposomes depended on the phospholipid used for reconstitution. (portlandpress.com)
Significantly1
Modulate1
  • Neurotransmitters modulate the SA node discharge rate by stimulation of beta-adrenergic and muscarinic receptors. (medscape.com)
Effect1
  • however, this effect had a slower onset in comparison to typical MAPK stimulation. (nih.gov)
Mobilization1
  • The expression of Egr2 and FasL, and the phosphorylation of AKT and ERK, after ionomycin and PMA co-stimulation, was detected, while the Ca 2+ mobilization stimulated by K + solution was determined. (researchsquare.com)
Expression2
  • NFAT2 overexpression induced the expression of Egr2 and FasL and suppressed the phosphorylation of AKT and ERK. (researchsquare.com)
  • We will use genetic tools to knock out genes and study the response of these mutants to touch stimulation in comparison to WT with regards to gene expression, phosphorylation cascades and alteration in growth and development. (lu.se)
Activation1
  • In contrast, both vasopressin and angiotensin 2-mediated stimulation of ERK activation was enhanced in SHR. (aspetjournals.org)
Response2
  • Some of the induced genes, such as CREM, CHOP, and MAP kinase phosphatase-1, may be components of a homeostatic response to excessive stimulation. (jneurosci.org)
  • In addition, we found that Wnt-5a could directly trigger a cAMP response that resulted in phosphorylation of DARPP-32 and stimulation with Wnt-5a was necessary for DARPP-32 mediated inhibition of cell migration in wound healing assay. (lu.se)
Regulatory1
Molecular1
  • Here, we used mass spectrometry (MS)-based methods to determine and quantify, in a time-dependent manner, early molecular events triggered following TCR stimulation with peptides of varying affinities. (nature.com)
Found2
  • In contrast with previous results of Furukawa & Nakamura [(1987) J. Biochem (Tokyo) 101, 287-290], no convincing evidence for a phosphorylation of the (Ca2+ + Mg2+)-ATPase was found. (portlandpress.com)
  • In this study, L-selectin was found to be phosphorylated in lymphoblastoid cell lines, and phosphorylation was enhanced by phorbol ester (phorbol 12-myristate 13-acetate (PMA)) treatment. (upenn.edu)
Effects1
  • In this paper we will examine the effects of the repeated electromagnetic field stimulation (REMFS) on cell cultures, mouse models, and computer simulations for diagnostic purposes. (intechopen.com)
State1
  • Our results showing that only dephosphorylated (activated) cofilin binds with F-actin support previous observations that the state of cofilin phosphorylation determines its association with F-actin. (uni-muenchen.de)
Values1
  • The degree of phosphorylation was unaffected by increasing MOCA concentrations with higher concentrations resulting in normal histone phosphorylation values for all histone groups with the exception of H3, which remained elevated following exposure to concentrations greater than 10microM. (cdc.gov)
Group1
  • After dimerization, the kinase is tagged with a marker called a phosphate group (a cluster of oxygen and phosphorus atoms) in a process called phosphorylation. (medlineplus.gov)