• Apoptosis
  • In the present study, we investigated whether the chronic effects of Ang II via AT1 receptor (AT1R) would result in endoplasmic reticulum (ER) stress/PKC-delta/p38 MAPK stimulation, and consequently podocyte apoptosis. (springer.com)
  • Together, our results indicate that Ang II-induced podocyte apoptosis is associated with AT1R/ER stress/PKC-delta/p38 MAPK axis and enhanced NHE1-mediated pHi recovery rate. (springer.com)
  • Results: In podocytes, PAN stimulated expressions of survivin and the apoptosis related molecule caspase 3. (bjmu.edu.cn)
  • Knockdown of survivin expression by siRNA increased the activation of caspase 3, induced podocyte apoptosis and remarkable rearrangement of actin cytoskeleton. (bjmu.edu.cn)
  • Moreover, over-expression of survivin inhibited PAN-induced podocyte apoptosis and cytoskeleton rearrangement. (bjmu.edu.cn)
  • Conclusion: Our data provides the evidence that survivin plays an important role in protecting podocytes from apoptosis induced by PAN. (bjmu.edu.cn)
  • In this episode, Alessia Fornoni reveals that TNF promotes free cholesterol-dependent podocyte apoptosis via an NFATc1/ ABCA1-dependent mechanism. (jci.org)
  • Exogenous TNF administration or local TNF expression was equally sufficient to cause free cholesterol-dependent apoptosis in podocytes by acting through a dual mechanism that required a reduction in ATP-binding cassette transporter A1-mediated (ABCA1-mediated) cholesterol efflux and reduced cholesterol esterification by sterol- O -acyltransferase 1 (SOAT1). (jci.org)
  • TNF-stimulated free cholesterol-dependent apoptosis in podocytes was mediated by nuclear factor of activated T cells 1 (NFATc1). (jci.org)
  • cells
  • This blot shows control (C) basal cells, insulin-stimulated podocytes, and phosphorylation of PKB [phosphorylated (p)PKB]. (nih.gov)
  • Podocytes are specialised cells forming an important part of the glomerular filtration barrier. (biomedcentral.com)
  • However, other groups have challenged those findings by demonstrating that podocytes express ACE2, but not ACE, suggesting that pathways opposing ANG II synthesis may be more active in these cells ( 54 ). (physiology.org)
  • Active ROCK in these cells seems to stimulate the disruption of E-Cadherin-mediated cell-cell contacts by activating actomyosin contractility. (wikipedia.org)
  • Inflammation that affects the cells surrounding the glomerulus, podocytes, increases the permeability to proteins, resulting in an increase in excreted proteins. (wikipedia.org)
  • It was shown to stimulate EMT in some systems by promoting/maintaining stemness of cancer cells, as is the case in pancreatic cancer, head and neck cancer, endometrial cancer, nasopharyngeal cancer, prostate cancer and non-small lung cancer. (wikipedia.org)
  • mechanism
  • Our data implicate an NFATc1/ABCA1-dependent mechanism in which local TNF is sufficient to cause free cholesterol-dependent podocyte injury irrespective of TNF, TNFR1, or TNFR2 serum levels. (jci.org)
  • pathway
  • differentiated mouse podocytes responded to insulin predominantly through the phosphatidylinositol 3-kinase (PI3K) pathway. (nih.gov)
  • Conditionally-immortalised human podocyte cell lines were cultured to examine expression of TLRs and the effects of their activation on the NFkB pathway and cell proliferation. (biomedcentral.com)
  • expression
  • Minimal change disease (MCD) is the most common cause of nephrotic syndrome in children and is associated with the expression of CD80 in podocytes and the increased excretion of CD80 in urine. (springer.com)
  • We hypothesized that serum from patients with MCD might stimulate CD80 expression in cultured podocytes. (springer.com)
  • Sera from MCD patients in relapse, but not in remission, stimulated CD80 expression in cultured podocytes. (springer.com)
  • No role in CD80 expression by podocytes was found for cytokines released by PBMCs. (springer.com)
  • Biopsies from LN patients have demonstrated higher TLR7 and TLR9 expression in glomeruli compared with controls and suggest that these TLRs are localised to podocytes. (biomedcentral.com)
  • Treatment of podocytes with LPS was associated with increased expression of TLR7 at both mRNA and protein levels whereas there was comparatively little change in TLR9. (biomedcentral.com)
  • Exposure of the podocytes to dexamethasone was associated with lower expression of TLR7 and TLR9 at the mRNA level. (biomedcentral.com)
  • Cyto-protection function of survivin was also studied in cultured podocyte injured by puromycin aminonucleoside (PAN), transfected with survivin siRNA to down-regulate survivin expression, or with survivin plasmid to transiently over-express survivin. (bjmu.edu.cn)
  • It is also more widely expressed in the earlier developing kidney, but again including definite podocyte expression. (wikipedia.org)
  • Klf4 was required for accumulation of β-catenin at the Tert promoter but was unable to stimulate Tert expression in the absence of β-catenin. (wikipedia.org)
  • sera
  • Immortalized human podocytes were incubated with culture media containing patient sera or supernatants from patient and control PBMC cultures. (springer.com)
  • We cultured human podocytes with sera collected from DKD patients, who displayed elevated TNF levels, and focal segmental glomerulosclerosis (FSGS) patients, whose TNF levels resembled those of healthy patients. (jci.org)
  • role
  • This research aims to examine the role of TLR7 and TLR9 in podocytes to identify potential targets for more effective therapies of lupus nephritis. (biomedcentral.com)
  • The Role of Survivin in Podocyte Injury Induced by Puromycin Aminonucleoside[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2014,15(4):6657-6673. (bjmu.edu.cn)
  • 2014).The Role of Survivin in Podocyte Injury Induced by Puromycin Aminonucleoside. (bjmu.edu.cn)
  • human
  • Insulin directly increased VEGF-A mRNA levels and protein production in conditionally immortalized wild-type human and murine podocytes. (nih.gov)
  • 2. Other functions and targets RhoA-GTP stimulates the phospholipid phosphatase activity of PTEN (phosphatase and tensin homologue), a human tumor suppressor protein. (wikipedia.org)
  • cultures
  • No such CD80 increase was observed when podocytes were incubated with supernatants of PBMC cultures from patients in relapse. (springer.com)
  • conditions
  • Modulation of VEGF-A by insulin in the podocyte may be another important factor in the development of glomerular disease associated with conditions in which insulin signaling to the podocyte is deranged. (nih.gov)
  • recent
  • Recent reports suggest that podocytes may contain an intrinsic RAS and endogenously form ANG II without the addition of exogenous substrate ( 15 , 30 ). (physiology.org)