Stem cell mesenchymal stromal cells. Medical search. Frequent questions

Despite almost 50 years of research and over 20 years of preclinical and clinical studies, the question of curative potential of mesenchymal stem/stromal cells (MSCs) is still widely discussed in the scientific community. (frontiersin.org)
In this mini review, we will discuss point by point possible pitfalls in the production of human MSCs for cell therapies, without consideration of material-based applications. (frontiersin.org)
Only a careful consideration and standardization of all pretreatment processes/methods for the different applications of MSCs will ensure robust and reproducible performance of these cell populations in the different experimental and clinical settings. (frontiersin.org)
Mesenchymal stem/stromal cells (MSCs) have the capacity to differentiate into cells and tissues of one germ layer, here the mesodermal lineage, and are consequently multipotent. (frontiersin.org)
Mesenchymal stem/stromal cells (MSCs) obtained from pluripotent stem cells (PSCs) constitute an interesting alternative to classical MSCs in regenerative medicine. (unlp.edu.ar)
Among their many mechanisms of action, MSC extracellular vesicles (EVs) are a potential suitable substitute for MSCs in future cell-free-based therapeutic approaches. (unlp.edu.ar)
Mesenchymal stem/stromal cells (MSCs)‐based therapy brings the reassuring capability to regenerative medicine through their self‐renewal and multilineage potency. (biomedcentral.com)
Although there is no particular quantitative assay to provide MSCs identification in mixed cells population [ 8 ], the International Society for Cellular Therapy (ISCT) has provided minimum principles to determine MSCs. (biomedcentral.com)
However, natural MSCs in vivo survival and their biological effects on tissue recovery decrease with long-term cultivation called aging and also injected cells demonstrate poor targeted migration [ 21 ]. (biomedcentral.com)
Mesenchymal stem/stromal cells (MSCs) are increasingly used as an intravenously applied cellular therapeutic. (stemcellslab.net)
Later, Arnold Caplan's group described mesenchymal stem/stromal cells (MSCs) as multipotent mesenchymal cell populations which can differentiate into several tissue types, and demonstrated roles for MSCs in the regeneration of bone, cartilage or ligaments in animal and clinical studies [2-4]. (stemcellslab.net)
Pivotal studies by the group of Horwitz in children with osteogenesis imperfecta, an inherited enzyme deficiency of collagen synthesis by mesenchymal cells in bone, opened the field for intravenous use of MSCs. (stemcellslab.net)
The tissue source of the MSCs was in most cases not decisive, and cells from various tissue sources were explored. (stemcellslab.net)
and signals from the injected cells were found early after administration of the MSCs at the highest frequencies in the lungs, followed by liver and spleen. (stemcellslab.net)
Mesenchymal stem cells (MSCs) are an adult stromal cell population possessing powerful differentiation capacity and a prospect of use across main histocompatibility complicated barriers. (biongenex.com)
Because of the immunological difficulties associated with allogeneic transplantation mesenchymal stem cells (MSCs) have the potential to be an attractive tissue replacement therapy for a number of reasons. (biongenex.com)
MSCs are multipotent cells that can be readily isolated from a number of adult tissues including bone marrow umbilical cord blood adipose tissue and placenta. (biongenex.com)
They have been well documented to differentiate into osteogenic adipogenic and chondrogenic lineages by activation of the cells through encountering inflammatory cytokines such as IFN-γ [21 23 Allogeneic MSCs from young healthy donors are an attractive source of regenerative cells for the treatment of degenerative diseases with an inflammatory component. (biongenex.com)
As MSCs possess potent immunomodulatory properties and an ability to differentiate into several lineages there is potential for allogeneic 'off the shelf' tissue engineering solutions using these cells. (biongenex.com)
Mesenchymal Stromal Cells (MSCs) are susceptible to tumor signalling and can be recruited to tumor regions. (unicyte.ch)
We found that CSC-derived EVs promoted persistent phenotypical changes in MSCs characterized by an increased expression of genes associated with cell migration (CXCR4, CXCR7), matrix remodeling (COL4A3), angiogenesis and tumor growth (IL-8, Osteopontin and Myeloperoxidase). (unicyte.ch)
Moreover, EV-stimulated MSCs enhanced migration of renal tumor cells and induced vessel-like formation. (unicyte.ch)
In vivo, EV-stimulated MSCs supported tumor development and vascularization, when co-injected with renal tumor cells. (unicyte.ch)
Bone marrow-derived mesenchymal stromal cells (MSCs) are known by their migratory capacity to injury and tumor sites. (unicyte.ch)
Anti- tumoral effects have been reported in different types of tumors, showing a reduction in tumor growth and metastasis with the presence of MSCs or EVs derived from these cells [15-18]. (unicyte.ch)
Such dual role possibly depends on a complex mechanism of interaction between tumor cells and MSCs that can be initially triggered by tumor EVs. (unicyte.ch)
Because of their multi/pluripotency and immunosuppressive properties, mesenchymal stem/stromal cells (MSCs) are important tools for treating immune disorders and for tissue repair. (biomedcentral.com)
Producing cells according to Good Manufacturing Practices (GMPs) is a global challenge for the production of all cells for use in humans, specifically mesenchymal stem/stroma cells (MSCs), one of the most promising tools in cellular therapy and regenerative medicine. (biomedcentral.com)
MSCs with a mesodermic origin were first described by Alexander Friedenstein and colleagues in the 1960s and 1970s[ 1 ] as non-hematopoietic bone marrow (BM) cells that adhered to plastic and that could develop into colonies with a fibroblastic appearance. (biomedcentral.com)
Cells referred to as MSCs originating from various tissues are now used in clinical trials. (biomedcentral.com)
Breast cancer progression is promoted by stromal cells that populate the tumors, including cancer-associated fibroblasts (CAFs) and mesenchymal stem/stromal cells (MSCs). (nih.gov)
TNF-α (and IL-1β) induced the release of CCL2, CXCL8 and CCL5 by MSCs and CAFs generated by prolonged stimulation of MSCs with Tumor CM of MDA-MB-231 and MCF-7 cells. (nih.gov)
Exposure of MSCs to TNF-α led to potent CCL2-induced migration of monocytic cells, a process that may yield pro-cancerous myeloid infiltrates in breast tumors. (nih.gov)
Here, by using a microarray analysis, we demonstrate that human BM mesenchymal stromal/stem cells (MSCs) in an early osteoinductive stage (e-MSCs) are characterized by unique hematopoiesis-associated gene expression with an enhanced hematopoiesis-supportive ability. (elsevierpure.com)
In comparison to BM-MSCs without osteoinductive treatment, gene expression in e-MSCs was significantly altered in terms of their cell adhesion- and chemotaxis-related profiles, as identified with Gene Ontology and Gene Set Enrichment Analysis. (elsevierpure.com)
Noteworthy, expression of the hematopoiesis-associated molecules CXCL12 and vascular cell adhesion molecule 1 was remarkably decreased in e-MSCs. (elsevierpure.com)
e-MSCs supported an enhanced expansion of CD34 + hematopoietic stem and progenitor cells, and generation of myeloid lineage cells in vitro. (elsevierpure.com)
e-MSCs exhibited the absence of decreased stemness-associated gene expression, increased osteogenesis-associated gene expression, and apparent mineralization, thus maintaining the ability to differentiate into adipogenic cells. (elsevierpure.com)
Our findings demonstrate the unique biological characteristics of e-MSCs as hematopoiesis-regulatory stromal cells at differentiation stage between MSCs and osteoprogenitor cells and have significant implications in developing new strategy for using pharmacological osteoinductive treatment to support hematopoiesis in hematopoietic stem and progenitor cell transplantation. (elsevierpure.com)
Recently, mesenchymal stem cells (MSCs) have been suggested for use in the cell-based treatment of cartilage lesions. (researchgate.net)
MSCs versus stem cell-based interventions other than MSCs. (lu.se)

Cell quality, as assessed by differentiation, cell surface marker expression and clonogenic ability, was retained post expansion on the liquid microcarriers. (aston.ac.uk)
They also can inspire angiogenesis by direct differentiation, cell-to-cell interaction, or paracrine effects. (biomedcentral.com)
The stromal cell system has been proposed to consist of marrow mesenchymal stem cells that are capable of self-renewal and differentiation into various connective tissue lineages. (nih.gov)
Recent data from in vitro models demonstrating the essential role of stromal support in hematopoiesis shaped the view that cell-cell interactions in the marrow microenvironment are critical for normal hematopoietic function and differentiation. (nih.gov)
Donor variation among patients, cell heterogeneity, and unpredictable capacity for differentiation reduce effectiveness of BMSCs for regenerative cell therapies. (elsevierpure.com)
The cell surface glycoprotein CD24 exhibits the most prominent differential expression during osteogenic versus adipogenic differentiation of human BMSCs. (elsevierpure.com)
In undifferentiated human BMSCs, CD24 cell surface expression is variable among donors (range: 2%-10%) and increased by two to fourfold upon osteogenic differentiation. (elsevierpure.com)
Strikingly, FACS sorted CD24pos cells exhibit delayed mineralization and reduced capacity for adipocyte differentiation. (elsevierpure.com)
Phenotype switching: plasticity and/or differentiation of stromal cells and their progenitors within the tumour microenvironment regulate tumour fate. (europa.eu)
The cells were phenotypically categorized according to the expression of CD29, CD90, CD34 and CD45, and submitted to adipogenic and chondrogenic differentiation for 21 days and osteogenic differentiation for 7, 14 and 21 days. (scielo.br)
On days 7, 14 and 21 of osteogenic differentiation, the cultures were submitted to evaluations of MTT conversion in formazan, of alkaline phosphatase activity (AP), of collagen and mineralized matrix synthesis, evaluation of the number of cells per field and there was quantification of the gene transcripts for osterix, bone sialoprotein (BSP), osteonectin (ON) and osteocalcin (OC). (scielo.br)
The number of cells per field, the AP activity, the collagen and mineralized matrix synthesis were higher in AT-MSC en differentiation, in relation to BM-MSC under the same conditions in all evaluated times. (scielo.br)
Is Adipocyte Differentiation the Default Lineage for Mesenchymal Stem/Progenitor Cells after Loss of Mechanical Loading? (scirp.org)
Hart, D. (2014) Is Adipocyte Differentiation the Default Lineage for Mesenchymal Stem/Progenitor Cells after Loss of Mechanical Loading? (scirp.org)
Ando, W., Heard, B., Nakamura, N., Frank, C.B. and Hart, D.A. (2012) Ovine Synovial Membrane-Derived Mescenchymal Progenitor Cells Retain the Phenotype of the Original Tissue That Was Exposed to in Vivo Inflammation: Evidence for a Suppressed Chondrogenic Differentiation Potential of the Cells. (scirp.org)
Conclusions: This study showed that an osteoblastic cell lineage may be obtained from human bone marrow derived from adherent cells, and that the presence of the rhBMP-4 seems to have an effect during the first stages of differentiation only. (bvsalud.org)
Temporal multimodal single-cell profiling of native hematopoiesis illuminates altered differentiation trajectories with age. (lu.se)

Sozzi E, Nilsson F, Kajtez J, Parmar M, Fiorenzano A. Generation of Human Ventral Midbrain Organoids Derived from Pluripotent Stem Cells . (lu.se)

In most cases, clinically relevant cell numbers for MSC-based therapies can be only obtained by in vitro expansion of isolated cells. (frontiersin.org)
Restored in vivo-like membrane lipidomics positively influence in vitro features of cultured mesenchymal stromal/stem cells derived from human placenta. (remembrane.com)
Cells derived from bone marrow can undergo osteoinduction in vitro in the absence of osteoinductive factors such as bone morphogenetic proteins. (bvsalud.org)
As a third major line of research, we explore, in collaboration with associate professor Henrik Ahlénius group if GBM cells can be reprogrammed to neuron like cells in vitro and in vivo. (lu.se)

The aim of this study was to compare the osteogenic potential of mesenchymal stem cells obtained from bone marrow (BM-MSC) with those extracted from adipose tissue (AT-MSC) of adult dogs. (scielo.br)

Stromal cells originate from multipotent mesenchymal stem cells. (wikipedia.org)

Also, following transplantation and migration to target tissue, they encounter a harsh milieu accompanied by death signals because of the lack of proper tensegrity structure between the cells and matrix. (biomedcentral.com)
In these studies, however, transplanted cells were followed, if at all, at the site of transplantation, and biodistribution was not an issue. (stemcellslab.net)
This concept started from the observation that bone marrow transplantation can provide stromal cells able to synthesize intact collagen type I, replacing deficient patient cell function and ameliorating disease symptoms [5]. (stemcellslab.net)
Data from preclinical transplantation studies suggested that stromal cell infusions not only prevent the occurrence of graft failure, but they have an immunomodulatory effect. (nih.gov)
Preclinical and early clinical safety studies are paving the way for further applications of mesenchymal stem cells in the field of transplantation with respect to hematopoietic support, immunoregulation, and graft facilitation. (nih.gov)
Likewise, many children are now surviving hematopoietic stem-cell transplantation (HSCT) (see related histology slide below) and require structured long-term follow-up care. (medscape.com)
To stimulate and facilitate further research, the NCI and NHLBI held the First International Consensus Conference on Late Effects after Pediatric Hematopoietic Cell Transplantation on April 28 and 29, 2011. (medscape.com)

Recently, the adipose tissue-derived stromal vascular fraction has become the most popular source for obtaining mesenchymal stem cells because it is less expensive and is easier to perform than bone marrow concentrate harvesting. (balkanmedicaljournal.org)
However, no study has investigated the factors affecting the mesenchymal stem cell population in adipose tissue derived stromal vascular fraction. (balkanmedicaljournal.org)
Understanding the interaction of patient factors with the mesenchymal stem cell count and cell viability in adipose tissue-derived stromal vascular fraction could provide crucial information for surgeons to improve patient selection and outcomes. (balkanmedicaljournal.org)
To evaluate the factors affecting the mesenchymal stem cell count, total cell count, and cell viability in adipose tissue-derived stromal vascular fraction. (balkanmedicaljournal.org)
This study retrospectively reviewed the medical records of 30 patients who underwent liposuction to harvest adipose tissue-derived stromal vascular fraction at our stem cell center. (balkanmedicaljournal.org)

Researchers at the Tisch Multiple Sclerosis Research Center of New York chose to focus their research efforts on a particular type of stem cell, namely bone marrow-derived mesenchymal stromal cells. (stemedix.com)
Bone marrow-derived mesenchymal stem/stromal cells (BMSCs) are fundamental to bone regenerative therapies, tissue engineering, and postmenopausal osteoporosis. (elsevierpure.com)
Borlongan, C.V., Glover, L.E., Tajiri, N., Kaneko, Y. and Freeman, T.B. (2011) The Great Migration of Bone Marrow-Derived Stem Cells towards the Ischemic Brain: Therapeutic Implications for Stroke and Other Neurological Disorders. (scirp.org)
Human bone marrow derived mesenchymal stromal cells (hMSC) is an excellent source, as these cells can be genetically modified to express proteins/drugs and also have an exceptional migratory or homing ability towards tumors, injury or inflammation. (brainsciencefoundation.org)

This is a randomised, double-blinded, placebo-controlled, parallel-group, prospective, single-centre trial investigating the safety , tolerability, and effectiveness of allogeneic stem cells as a treatment for radiation -induced hyposalivation and xerostomia for previous head and neck cancer patients . (bvsalud.org)
Participants will be randomly assigned using block randomisation to one of two parallel groups in a 11 ratio to receive ultrasound-guided injection of allogeneic adipose-derived mesenchymal stem cell (ASC) (n = 60) or placebo (n = 60) into the submandibular glands . (bvsalud.org)

Extracellular vesicles derived from renal cancer stem cells induce a pro-tumorigenic phenotype in mesenchymal stromal cells. (unicyte.ch)
Advances in this field point to a more complex model of tumor development than previously envisioned, in which the stem-like phenotype may be dynamically acquired by cancer cells. (hindawi.com)

The cells were first named colony-forming unit-fibroblasts (CFU-Fs). (biomedcentral.com)

Exosomes secreted by the umbilical cord mesenchymal stromal cells (UMSCs) and circular RNAs (circRNAs) derived from exosomes have been shown to be associated with the progression of GDM-related complications. (hindawi.com)
Batsali, A.K., Kastrinaki, M.C., Papadaki, H.A. and Pontikoglou, C. (2013) Mesenchymal Stem Cells Derived from Wharton's Jelly of the Umbilical Cord: Biological Properties and Emerging Clinical Applications. (scirp.org)

The molecular study of fat cell development in the human body is essential for our understanding of obesity and related diseases. (mdpi.com)
BACKGROUND: Traditional large-scale culture systems for human mesenchymal stem/stromal cells (hMSCs) use solid microcarriers as attachment substrates. (aston.ac.uk)
Further secondary endpoints are safety and immune response ( human leukocyte antigen (HLA) response) to the stem cells will be assessed. (bvsalud.org)
Over the past decade, the identification of stem-like cells as drivers of tumor initiation, recurrence, and metastasis spread in a variety of human cancers had a tremendous impact in basic and translational cancer research. (hindawi.com)
Hence, expression of the cell surface glycoprotein CD24 identifies a subpopulation of human BMSCs with reduced capacity for proliferation and extracellular matrix mineralization. (elsevierpure.com)
To further explore the role of PZR, we assessed the expression of PZR and its ITIM-less isoform, PZRb, in human bone marrow mesenchymal stromal cells (hBM MSC), and its ability to facilitate adhesion to and spreading and migration on various ECM molecules. (ox.ac.uk)
Human placenta-derived mesenchymal stromal cells (PMSCs) -- known for their neuroprotective qualities -- were preserved in hydrogel and applied to the site of the lesion. (sciencedaily.com)
We further demonstrated successful examples of multiple targeting including chromosomal deletions in human cells using the all-in-one CRISPR/Cas9 vectors constructed with our novel system. (nature.com)
The Ottawa Hospital's Biotherapeutics Manufacturing Centre (BMC) is the most experienced and successful facility of its kind in Canada, having manufactured more than a dozen different cell and virus-based products for human clinical trials in Canada, the United States, Europe and Asia. (ohri.ca)
By contrast, somatic alterations in the genes encoding Notch signaling components drive various types of human cancer, such as breast cancer, small-cell lung cancer (SCLC) and T-cell acute lymphoblastic leukemia (T-ALL) ( 6 - 9 ). (spandidos-publications.com)
Frederick, Maryland (PRWEB) July 06, 2017 -- RoosterBio Inc, today, announces the launch of RoosterReplenish™-MSC-XF, a first-in-class bioreactor feed designed to economically and rapidly scale up 3D culture of its standardized, well-characterized high-quality bioprocess hMSC (human mesenchymal stem/stromal cell) banks. (prweb.com)
RoosterBio's products are high volume, affordable, and well-characterized adult human mesenchymal stem/stromal cells (hMSCs) paired with highly engineered media systems. (prweb.com)
Gimble, J.M., Bunnell, B.A. and Guilak, F. (2012) Human Adipose-Derived Cells: An Update on the Transition to Clinical Translation. (scirp.org)
This project will study the use genetically modified human mesenchymal stromal cells as a local therapeutic drug delivery vehicle for the targeted delivery and secretion of chemotherapeutic/biologic agents for treatment of brain tumors. (brainsciencefoundation.org)
Objective: This study evaluated the osteogenic induction of human bone marrow cells by human recombinant bone morphogenetic protein-4 (rhBMP-4) and proteins released by Saos-2 (human osteosarcoma cell line). (bvsalud.org)
Study design: Osteoinduction in the presence or absence of Saos-2 and/or rhBMP-4 was evaluated in cultured human bone marrow cells. (bvsalud.org)
As human osteosarcoma cells are a source of additional growth they did not affect osteoinduction. (bvsalud.org)
Osteoblasts were obtained from human bone marrow cells even in the absence of growth factors and showed a typical morphology. (bvsalud.org)
We have already acquired proof-of-principle showing that reprogramming towards astrocytic fate causes morphological changes, reduces proliferation and bestow astrocytic function in human GBM cell lines. (lu.se)
The applicant is responsible for a brain tumor tissue biobank which provides the basis for the studies on human GBM tumor cells and stroma. (lu.se)

After that, Kaplan firstly proposes the term "mesenchymal stem cells," which are cells isolated from fully developed bone marrow (BM) that can usually differentiate into several types of mesenchymal origin cells [ 4 ]. (biomedcentral.com)

The results at the conclusion of this project will provide us all the information necessary for a pilot phase I feasibility and safety study for treating brain tumor patients with mesenchymal stem cells that have been engineered to express cytosine deaminase (MSC-CD) followed by 5-FC therapy. (brainsciencefoundation.org)

Clinical Relevance: The harvest technique of BM may be of major importance in determining the clinical success of BM mesenchymal stem/stromal cells in cartilage repair. (whiterose.ac.uk)
Impressively, four of the six patients-for whom no other multiple sclerosis treatment worked-had a measurable clinical improvement after stem cell treatment. (stemedix.com)
Based on the results of this clinical study, the scientists concluded that neural progenitor cells created from autologous mesenchymal stromal cells were safe to use in patients with primary progressive multiple sclerosis. (stemedix.com)
2016). Clinical safety of intrathecal administration of mesenchymal stromal cell-derived neural progenitors in multiple sclerosis. (stemedix.com)
The relationship of patient factors (age, body mass index, hypertension, and coronary artery disease) with cell viability and mesenchymal stem cell counts may be important for clinical applications. (balkanmedicaljournal.org)
Phase III clinical trials of Rova‑T for patients with small‑cell lung cancer and a phase III clinical trial of nirogacestat for patients with desmoid tumors are ongoing. (spandidos-publications.com)
Translational scientists and process and product developers moving towards clinical translation, and eventual commercialization, require robust, reproducible, scalable cell culture manufacturing platforms that lower regulatory burden and that can be implemented with a commercially-viable Bill of Materials. (prweb.com)
hMSC is an ideal candidate and compatible for clinical use as these cells can be harvested by bone marrow aspiration from individual patients without difficulty, processed ex vivo very efficiently, and later transplanted back into the same patients during surgery thereby reducing immunological consequences. (brainsciencefoundation.org)

and were called hematopoiesis-supporting cells in BM [ 3 ]. (biomedcentral.com)
In the 1970s, Friedenstein and colleagues [1] first reported that locally applied culture-expanded populations of bone marrow stroma-derived fibroblastic cells remained at their injection sites under the kidney capsule, where an ectopic hematopoiesis was initiated. (stemcellslab.net)
There has been an increasing interest in recent years in the stromal cell system functioning in the support of hematopoiesis. (nih.gov)

Functional specialization among BMSCs populations may support their regenerative potential and therapeutic success by accommodating cell activities that promote skeletal tissue formation, homeostasis, and repair. (elsevierpure.com)
This therapeutic strategy employs genetic modification of hMSC and exploits the tumor tropic behavior, for tracking to sites of tumor cell invasion and allows on-site generation of drug to control tumor growth. (brainsciencefoundation.org)
Stem cell-based therapies offer a potential therapeutic approach to repair, restore, or regenerate injured brain tissue. (lu.se)

Migration of monocytic cells was determined in modified Boyden chambers. (nih.gov)
PZR promotes cell migration on the extracellular matrix (ECM) molecule, fibronectin, by interacting with SHP-2 (Src homology-2 domain-containing protein tyrosine phosphatase-2), a molecule essential for skeletal development and often mutated in Noonan and Leopard syndrome patients sharing overlapping musculoskeletal abnormalities and cardiac defects. (ox.ac.uk)
Li, L. and Jiang, J. (2011) Regulatory Factors of Mesenchymal Stem Cell Migration into Injured Tissues and Their Signal Transduction Mechanisms. (scirp.org)

But the cartilage regeneration capacity of these cells remains unpredictable because of cell heterogeneity. (whiterose.ac.uk)
Hypothesis: The harvest technique of BM may highly influence stem cell heterogeneity and, thus, cartilage formation because these cells have distinct spatial localization within BM from the same bone. (whiterose.ac.uk)
Understanding how cancer stem cells affect tumor heterogeneity, the contribution of resident stem and progenitor cells to the tumor niche, and the search for true cancer stem cell markers are other examples of scientific challenges in this fast-growing research area. (hindawi.com)
Hart, D.A. (2014) Why Mesenchymal Stem Cell/Progenitor Cell Heterogeneity in Specific Environments? (scirp.org)

The interplay between stem cell and tumor biology offers an exceptional opportunity to advance our knowledge about cancer, one of the leading causes of death worldwide, with potential impacts in cancer diagnosis and therapy. (hindawi.com)

Renal carcinomas have been shown to contain a population of cancer stem cells (CSCs) that present self-renewing capacity and support tumor growth and metastasis. (unicyte.ch)
A subset of tumor cells, defined as cancer stem cells (CSCs), are characterized by self-renewal and continuous proliferation capacity, providing the ability to initiate tumor and to generate other heterogeneous cell populations [1]. (unicyte.ch)
Tumor survival and growth are associated with the capacity of cancer stem cells and of their progenies to interact with the surrounding stromal cells [2]. (unicyte.ch)
Effectiveness and safety of mesenchymal stem/stromal cell for radiation-induced hyposalivation and xerostomia in previous head and neck cancer patients (MESRIX-III): a study protocol for a single-centre, double-blinded, randomised, placebo-controlled, phase II study. (bvsalud.org)
Our novel results emphasize the important roles of inflammation-stroma interactions in breast cancer, and suggest that NF-κB may be a potential target for inhibition in tumor-adjacent stromal cells, enabling improved tumor control in inflammation-driven malignancies. (nih.gov)
We invite investigators to submit original research as well as review articles addressing recent advancements in our understanding of stem cells in cancer. (hindawi.com)
Notch signaling is aberrantly activated in breast cancer, non‑small‑cell lung cancer and hematological malignancies, such as T‑cell acute lymphoblastic leukemia and diffuse large B‑cell lymphoma. (spandidos-publications.com)
However, Notch signaling is inactivated in small‑cell lung cancer and squamous cell carcinomas. (spandidos-publications.com)
Notch signaling cascades crosstalk with fibroblast growth factor and WNT signaling cascades in the tumor microenvironment to maintain cancer stem cells and remodel the tumor microenvironment. (spandidos-publications.com)
Small-molecule inhibitors, antagonistic monoclonal antibodies (mAbs), antibody-drug conjugates (ADCs), bispecific antibodies or biologics (bsAbs) and chimeric antigen receptor-modified T cells (CAR-Ts) targeting Notch signaling components have been developed as investigational anti-cancer drugs ( 10 - 12 ). (spandidos-publications.com)
Two major concerns in cancer therapy are the short half-life, availability or excessive toxicity of the drugs, and the extreme invasiveness of the malignant tumor cells to infiltrate into the surrounding tissue and recur locally near the neurosurgical resection site. (brainsciencefoundation.org)
Resolving the Pathogenesis of Anaplastic Wilms Tumors through Spatial Mapping of Cancer Cell Evolution. (lu.se)

The tumor is composed by different cell populations. (unicyte.ch)

The researchers harvested these cells from the patients themselves (autologous stem cells). (stemedix.com)

Dexter demonstrated in 1982 that an adherent stromal-like culture was able to support maintenance of hematopoietic stem as well as early B lymphopoeisis. (nih.gov)
The industry's first Xeno-free (XF) hMSC bioreactor feed, RoosterReplenish-MSC-XF rounds out RoosterBio's development grade product portfolio to support both suspension and adherent cell culture. (prweb.com)
Results: Bone marrow cells were adherent since the first day of culture and were positive for osteonectin. (bvsalud.org)

CSCs were shown to secrete large amount of extracellular vesicles (EVs) that can transfer several molecules (proteins, lipids and nucleic acids) and induce epigenetic changes in target cells. (unicyte.ch)
In addition, tumor cells interaction were also shown to be mediated by secretion of extracellular vesicles (EVs) that can transfer proteins, lipids and nucleic acids and induce epigenetic changes in target cells [4,5]. (unicyte.ch)

Recent efforts demonstrated that the multiple mesenchymal lineages can be clonally derived from a single mesenchymal stem cell, supporting the proposed paradigm. (nih.gov)
Such cells from different sources can proliferate and differentiate into different lineages (e.g. osteogenic, chondrogenic and adipogenic) after suitable stimulation. (scirp.org)

Mesenchymal stem/stromal cells (MSC) are the ideal source to study fat formation as they are the progenitors of adipocytes. (mdpi.com)
Background: Connective tissue progenitors (CTPs) from native bone marrow (BM) or their culture-expanded progeny, often referred to as mesenchymal stem/stromal cells, represents a promising strategy for treatment of cartilage injuries. (whiterose.ac.uk)
Then, in their laboratory, scientists used various means to prompt the cells to become neural progenitors. (stemedix.com)

Operative variables, such as lipoaspirate amount and donor areas from the stromal vascular fraction harvesting site, included the entire abdomen and lower abdomen. (balkanmedicaljournal.org)
We recorded the mesenchymal stem cell population, cell viability, and cell count of stromal vascular fraction, and we analyzed the results to determine statistical significance. (balkanmedicaljournal.org)

Unlike cells, EVs do not elicit acute immune rejection, and they can be produced in large quantities and stored until ready to use. (unlp.edu.ar)
To circumvent these adversities in the delivery of a viral vector to the site of pathology, mesenchymal stem/stromal cells may be used to avoid immune stimulation with resultant vector clearance, to maximize the effective delivery potential, and to augment biodistribution ability in neoplastic tumor masses. (northwestern.edu)
The lymph tissue in the lymph nodes consists of immune cells (95%), for example lymphocytes, and stromal cells (1% to 5%) The genesis of lymph nodes begins within the blood and the lymphatic system. (wikipedia.org)
Lymphocytes leave the lymph node, as effector immune cells, via the efferent lymph vessels. (wikipedia.org)
This means immune cells may leave the lymph node along a chemokine gradient. (wikipedia.org)
Its cytoplasmic tail contains two immune-receptor tyrosine-based inhibitory motifs (ITIMs), implicated in adhesion-dependent signaling and regulating cell adhesion and motility. (ox.ac.uk)
During the process of airway inflammation, complex interactions of innate and adaptive immune cells as well as structural cells and their cytokines have many important roles. (cdc.gov)

Interactions between stromal and hematopoietic cells are important for the development of lymph nodes. (wikipedia.org)

Downregulated genes are associated with cell cycle regulation, and biological assays revealed that CD24pos cells have reduced proliferation. (elsevierpure.com)
Dr. Menon's research is focused on developing new cell based drug delivery systems for continuous delivery of drug/biological agents at tumor site. (brainsciencefoundation.org)

Undifferentiated mesenchymal stem cells are pluripotent cells that can differentiate into various phenotypes and are a source of osteogenic Cells 1,2 . (bvsalud.org)

A fed-batch culture system removes the need to perform costly and labor-intensive half or full media exchanges and significantly improves the productivity of XF media over traditional batch culture methods (millions of cells produced per Liter of media consumed in 2D and 3D culture). (prweb.com)

CCL2 was expressed in CAFs residing in proximity to breast tumor cells in biopsies of patients diagnosed with invasive ductal carcinoma. (nih.gov)
The goal is the direct targeting and eradication of these disseminated tumor cells in the brain. (brainsciencefoundation.org)
This approach will allow the cells to migrate to dispersed tumor cells near the surgical site and also to track down tumor cells migrated to the normal brain parenchyma. (brainsciencefoundation.org)
The 5-FU produced locally will diffuse through cellular membranes thereby increasing the cytotoxicity to neighboring tumor cells without increasing the risk of harmful systemic effects. (brainsciencefoundation.org)

The molecular signaling systems (chemokines) that distribute lymphocytes to appropriate localities within the lymph node (T and B cell segregation) are also created by lymph node stromal cells. (wikipedia.org)
Wu, Y. and Zhao, R.C. (2012) The Role of Chemokines in Mesenchymal Stem Cell Homing to the Myocardium. (scirp.org)

The mechanisms involved in this crosstalk are based on cell-to-cell contact and on paracrine secretion of several molecules including growth factors, cytokines and inflammatory mediators [3]. (unicyte.ch)
In an inflammatory state, lymphatic endothelial cells increase their surface adhesion molecules, and dendritic cells express a surface CCR7 receptor. (wikipedia.org)
We found that SS enriched by a hAMSC-conditioned medium (hAMSC-CM) preserved the viability and delayed the apoptosis of alveolar epithelial cells (A549) through the downregulation of inflammatory factors and the upregulation of antiapoptotic factors. (xvivoperfusion.com)

Next, we compared both datasets and found that while iPSC EVs enclose proteins that modulate RNA and microRNA stability and protein sorting, PD-MSC EVs are rich in proteins that organize extracellular matrix, regulate locomotion, and influence cell-substrate adhesion. (unlp.edu.ar)
RNAseq analysis of CD24pos and CD24neg BMSCs identified a limited number of genes with increased expression in CD24pos cells that are associated with cell adhesion, motility, and extracellular matrix. (elsevierpure.com)

This study presents the validation of a method, quantifying the ability of MSC to inhibit T cell proliferation according to the ICH Q2 standard. (mendeley.com)
Two parameters were calculated: the percentage of inhibition (PI) of T cell proliferation, for each ratio X, determined using the following formula PI ratio X = (PD control - PD ratio X) / PD control, and the corresponding area under the curve (AUC). (mendeley.com)

Mesenchymal stem/stromal cells (MSC) have immunomodulatory properties, studied in a wide range of diseases. (mendeley.com)

The researchers provided between 2 to 5 infusions of neural progenitor cells into the spinal fluid. (stemedix.com)
Two courses of four weekly infusions of rituximab with or without interferon-2a: final results from a randomized phase III study in symptomatic indolent B-cell lymphomas. (cancercentrum.se)

For more information on RoosterBio and adult stem cells, please visit http://www.roosterbio.com , follow on twitter (@RoosterBio), or read our corporate blog ( http://www.roosterbio.blogspot.com ). (prweb.com)

Stem cell therapy has been the subject of many studies for its potential to cure many disorders. (biomedcentral.com)
Find out what potential options you have with stem cell therapy. (stemedix.com)
Intraglandular mesenchymal stem cell therapy has shown promising results as a treatment for xerostomia . (bvsalud.org)
The lower-limb paralysis associated with spina bifida may be effectively treated before birth by combining a unique stem cell therapy with surgery, new research has found. (sciencedaily.com)
high-risk patients included male subjects, recipients of stem cells from female donors, patients younger than 10 years, those with nonidentical donors, and those who received radiation therapy in the transplant preparative regimen. (medscape.com)

Since the iliac crest remains a frequent site of BM harvest for musculoskeletal regeneration, the authors also compared the spatial distribution of these subsets in trabeculae of femoral head and iliac crest and found CD2711CD561 bone-lining cells in both tissues. (whiterose.ac.uk)
Mesenchymal stem/progenitor cells (MSC/MPC) are found in many tissues and fluids including bone marrow, adipose tissues, muscle, synovial membranes, synovial fluid, and blood. (scirp.org)
Cells Tissues Organs. (cancercentrum.se)

Numerous reports showed that tumor EVs are not restricted only to tumor microenvironment but are also present in the blood circulation and other body fluids, supporting the idea that EVs can also stimulate cells at distant sites in the organism [10-13]. (unicyte.ch)
molecules secreted by the tumor and tumor-related stromal cells, promoting their recruitment from bone marrow into the circulation and subsequent engraftment within tumor microenvironment [14]. (unicyte.ch)

Stem cell-based therapies provide a promising approach for treatment of tumors in humans. (brainsciencefoundation.org)
Tissue from both primary surgery and reoperations from the same patients, allows in depth analysis of the evolution of glioblastoma over time with the goal of development of diagnostic monitoring and identification of druggable GBM stromal targets in recurrent brain tumors. (lu.se)

These sinuses are cavities containing macrophages (specialised cells which help to keep the extracellular matrix in order). (wikipedia.org)

A neural progenitor cell is a cell that can become any of the three main types of brain cells: neurons, astrocytes, or oligodendrocytes. (stemedix.com)

Starting with cell source, choice of donor and recipient, as well as isolation methods, we will then discuss existing expansion protocols (two-/three-dimensional cultivation, basal medium, medium supplements, static/dynamic conditions, and hypoxic/normoxic conditions) and influence of these strategies on the cell functionality after implantation. (frontiersin.org)
Results: Cells from the BM after rasping were significantly more chondrogenic than the donor-matched aspirate, whereas no notable difference in their osteogenic or adipogenic potential was observed. (whiterose.ac.uk)

Crosstalk LEC, lymphoid tissue inducer cells and mesenchymal stromal organizer cells initiate the formation of lymph nodes. (wikipedia.org)

The multiple sclerosis patients treated with the cells tolerated the treatment very well. (stemedix.com)
Moreover, the beneficial effect witnessed in two-thirds of treated patients suggests that these cells may be able to help patients with even the most severe and difficult-to-treat forms of multiple sclerosis. (stemedix.com)
These treatment options would significantly decrease costs reduce the number of procedures patients must undergo and provide cells from young healthy donors that may show higher efficacy than cells from aged individuals [24]. (biongenex.com)

Anatomy57

Organisms9

Diseases9

Chemicals and Drugs34

Analytical, Diagnostic and Therapeutic Techniques and Equipment39

Phenomena and Processes40

Disciplines and Occupations3

Technology, Industry, Agriculture2

MSCs41

Differentiation17

Pluripotent stem1

Vitro4

Potential of mesenchymal1

Multipotent mesenchymal1

Transplantation7

Adipose tissue5

Bone marrow-derived4

Allogeneic2

Phenotype2

Fibroblasts1

Umbilical cord2

Human20

Differentiate into several1

Brain tumor1

Clinical8

Hematopoiesis3

Therapeutic3

Migration3

Heterogeneity4

Biology1

Cancer12

Populations1

Autologous1

Adherent3

Vesicles2

Lineages2

Progenitors3

Vascular2

Immune7

Interactions1

Biological2

Phenotypes1

Improves1

Tumor cells4

Chemokines2

Inflammatory3

Adhesion2

Proliferation2

Immunomodulatory1

Infusions2

Adult1

Therapy5

Tissues3

Microenvironment2

Tumors2

Extracellular matrix1

Progenitor cell1

Donor2

Initiate1

Patients3