The bright cell with lots of orange on the right is in metaphase and the cell with lots of green on the left is in anaphase. (concordia.ca)
Metaphase and anaphase are both stages in mitosis and we study the mechanisms that govern them. (concordia.ca)
Metaphase (from the Greek μετά, "adjacent" and φάσις, "stage") is a stage of mitosis in the eukaryotic cell cycle in which chromosomes are at their second-most condensed and coiled stage (they are at their most condensed in anaphase). (askdifference.com)
Oocytes17
Aspiration of antral-stage follicles allowed the retrieval of 15 immature oocytes. (springer.com)
Parthenogenetic activation of metaphase II (MII) oocytes along with a mix of activating agents including ionomycin and 6-dimethylaminopurine (6-DMAP), stops the protrusion of the second polar body thereby allowing the activated oocytes to retain their entire genetic material. (pharmaceutical-technology.com)
Hypertonic medium treatment for localization of nuclear material in bovine metaphase II oocytes. (ox.ac.uk)
Oocytes enucleated at the second metaphase stage (MII) are often used as recipient cytoplasts for nuclear transfer. (ox.ac.uk)
A previous study showed that pretreatment of mouse oocytes with 3% (0.09 M) sucrose allowed visualization of the metaphase spindle and chromosomes under standard light microscopy and led to a 100% enucleation rate. (ox.ac.uk)
Staining of DNA with Hoechst 33342 revealed that these projections coincided with the position of the metaphasechromosomes in 100% of sucrose-treated oocytes, whereas only 31% of oocytes showed alignment of the position of Pb1 with their nuclear materials. (ox.ac.uk)
This study demonstrated that 0.3 M sucrose treatment of bovine oocytes facilitates the localization of metaphasechromosomes under normal light microscopy and hence increases enucleation efficiency without compromising the in vitro development potential of cloned embryos by nuclear transfer. (ox.ac.uk)
The cloning method is based on the fact that cytoplasmic factors in mature, metaphase II oocytes are able to reset the identity of a transplanted adult cell nucleus to an embryonic state. (news-medical.net)
The adult cell nuclei were transferred into metaphase-II stage human oocytes, producing a karyotypically normal diploid embryonic stem cell line from each of the adult male donor cells. (news-medical.net)
Most oocytes (77%) were arrested at a meiotic stage characterized by the presence of a large internal metaphase plate and absence of polar body. (techscience.com)
The aims of this study were to investigate the effect of oviductal cells on in vitro maturation (IVM) of canine oocyte in Tissue Culture Medium 199 (TCM-199) or synthetic oviductal fluid (SOF) supplemented with bovine serum albumin (BSA) or fetal calf serum (FCS) and to compare the maturation rates of oocytes from the diestrus and anestrus stages. (tubitak.gov.tr)
Dot1L was detected diffuse staining in the nuclear of mouse GV (Germinal Vesicle) stage oocytes. (nel.edu)
In Dot1L deficient, accompanying with BubR1 (MAD3/Bub1b) remains on the chromosome, the mouse oocytes was blocked in metaphase of meiosis I. The histone deacetylation was also incomplete in Dot1L-deficient mouse oocytes. (nel.edu)
Dot1L protein is well distributed in mouse GV stage oocytes. (nel.edu)
This report describes the application of a novel tyramine -linked hyaluronan for 3-D in vitro follicle culture and the production of developmentally competent metaphase II oocytes . (bvsalud.org)
Follicle growth , antrum formation and meiotic maturation to metaphase II oocytes was monitored. (bvsalud.org)
This culture model allowed ovulation of functionally competent metaphase II oocytes , capable of fertilization , genomic activation and blastulation. (bvsalud.org)
Interphase7
While in the trophozoite life stage and early cyst stage, the cell is in interphase. (wikipedia.org)
During metaphase the X-shaped structure is called a metaphase chromosome, which is highly condensed and thus easiest to distinguish and study. (wikipedia.org)
When the oocyte is in the metaphase II stage of cell division, the spindle-chromosome complex is removed and inserted into a healthy donor oocyte from which the nucleus has already been removed. (msdmanuals.com)
The three-dimensional relief model shows the 10 stages of meiosis on the basis of a typical mammal cell. (sargentwelch.com)
The first stage of meiosis, constituted by a series of events that include the thickening and coiling of the chromosomes, synapsis of homologous chromosomes, tetrad formation, and crossing over. (askdifference.com)
Mitotic spindle2
Metaphase starts when the mitotic spindle organizes all chromosomes and lines them up in the middle of the cell to divide. (vedantu.com)
It is presumed to stop the metaphase stage therefore preventing microtubule formation in the mitotic spindle. (medscape.com)
The vinca alkaloid agents are naturally occurring or semisynthetic nitrogenous bases that elicit their cytotoxic effects through tubulin-binding properties and disruption of microtubule function, which lead to metaphase arrest. (medscape.com)
Meiotic1
Meiotic maturation rate to the metaphase II stage was 73% (228/314). (bvsalud.org)
The advancement of imaging techniques by 1963 led to the observation of mitotic figures being dispersed throughout a cell's cytoplasm, rather than just being present during metaphase. (databasefootball.com)
Cell's1
Metaphase is the third of the five phases of biological cell division, or more specifically, the division of what is inside that cell's nucleus. (sciencing.com)
In humans, a major roadblock in achieving successful SCNT leading to embryonic stem cells has been the fact that human SCNT embryos fail to progress beyond the eight-cell stage. (news-medical.net)
This is still in experimental stages but has been done on several human embryos. (msdmanuals.com)
Cell division2
Chromosomes are normally visible under a light microscope only during the metaphase of cell division (where all chromosomes are aligned in the center of the cell in their condensed form). (wikipedia.org)
Using early imaging techniques, scientists observed these structures as being most pronounced during metaphase but then gradually disappearing during later stages of cell division. (databasefootball.com)
It has been known for over 125 years that fertilization results in the formation of a new genetically unique living single-cell human organism, a human embryo or human being at the single-cell stage. (lifeissues.net)
Germinal vesicle1
When compared between anestrus and diestrus stages for all parameters (undetermined nuclear material, germinal vesicles, germinal vesicle break down, metaphase I, metaphase II, and degenerated) in different media, the differences were found to be significant statistically in Group IIa (22.9%) and Group IIIb (35.7%) for the germinal vesicle stage (P (tubitak.gov.tr)
Cells5
Following the trophozoite stage, cells enter an obligatory digestive and a later reproductive cyst stage. (wikipedia.org)
The drug we are developing stops cancer cells from continuing to divide and arrests cells in metaphase. (concordia.ca)
Unlike induced pluripotent stem cells (iPSs), hPSCs do not manipulate the gene expression process back to a less differentiated stage, thereby avoiding the regulatory, ethical and safety obstacles. (pharmaceutical-technology.com)
Mitosis is the actual division stage where two daughter cells are created. (biolegend.com)
The spherical nuclei increase in size from 1.5-2.0 µm in the trophozoite stage to 2.5-3.5 µm. (wikipedia.org)
Adult3
Gonads (testes and ovaries) from adult insects (occa- sionally fifth-stage nymphs) were removed, fixed in ethano-acetic acid (3:1), and stored at -20°C. C-banding treatment was carried out on air-dried squash, as previous- ly described (13). (cdc.gov)
The theory that successive stages of individual development (ontogeny) correspond with ('recapitulate') successive adult ancestors in the line of evolutionary descent (phylogeny) became popular in the nineteenth century as the so-called biogenetic law. (lifeissues.net)
Indeed, the early stages in the development of an animal are not like the adult stages of other forms but resemble only the early stages of those animals. (lifeissues.net)
Divide1
Metaphase might best be conceived of as a step in which the elements of the soon-to-divide cell arrange themselves into a neat formation, like a tiny military platoon. (sciencing.com)
HR would depend on sister chromatids as layouts, which is limited to the S/G2 stages from the cell routine 5. (exposed-skin-care.net)
Human1
In 1942, the Carnegie Stages of Early Human Embryonic Development were instituted at the National Museum of Health and Medicine in Washington, D.C. 4 The Carnegie Stages of Early Human Development are the basis for the Nomina Embryologica which was part of the larger Nomina Anatomica for decades until 1989. (lifeissues.net)
Occurs3
Members of the genus alternate between two life stages: a free-living trophozoite stage and a cyst stage in which mitosis occurs. (wikipedia.org)
Orthomitosis in Vampyrella occurs late in the cyst stage. (wikipedia.org)
If this drug is indeed targeting a mechanism unique to highly progressive cancers, then it could provide an alternative treatment for patients in the late stages of their disease. (concordia.ca)
Model1
Following the stages of mitosis during instruction, this model can be set up to mimic the pictures in the book. (perkins.org)
Disease2
The combinations vary by the stage of disease and by the treating institution. (medscape.com)
In India most of patients with breast cancer diagnosed with advanced stage of the disease. (who.int)