Compatible with adherent and suspension cells, the animal-origin free Trans IT-X2® achieves both exceptional transient and stable transfections. (bioscience.co.uk)
We recommend using TransPass D2 ( NEB #M2554 ) in combination with TransPass V ( NEB #M2561 ) or Roche's FuGENE® 6 Transfection Reagent for both transient and stable transfections. (neb.com)
Plasmid4
TransI T-X2® from Mirus Bio is a low toxicity, dynamic transfection system for the superior transfection of plasmid DNA, siRNA, miRNA and CRISPR/Cas9 components into mammalian and primary cells. (bioscience.co.uk)
Typically, most of the cells that have not integrated the transfected plasmid will die while the cells that have undergone plasmid integration will survive by 9 days post-transfection. (mirusbio.com)
The procedure stated below is designed for the transfection of CHO cells with 1 µg/µL pSV40-CAT plasmid (diluted in sterile molecular biology grade water). (sigmaaldrich.com)
This plasmid is suitable for stable transfection. (neb.com)
Reagent5
The Effectene Transfection Reagent is intended for molecular biology applications. (qiagen.com)
Due to its low cytotoxicity, Effectene Transfection Reagent is highly suitable for transfection of primary cells and many sensitive cell lines. (qiagen.com)
Transfection using Effectene Transfection Reagent requires low amounts of DNA and minimal handling. (qiagen.com)
In addition, removal of transfection complexes is not required, making this reagent highly suitable for high-throughput screening. (qiagen.com)
Vectors4
Chemicals include methods such as lipofection, which is a lipid-mediated DNA-transfection process utilizing liposome vectors. (wikipedia.org)
Products offered include viral vectors produced by transient transfection method or in stable producer cells, vector purification, and non-GMP quality testing. (cincinnatichildrens.org)
The Viral Vector Core offers production of research-grade vectors, generation of stable producer lines, and non-GMP quality control testing including vector titer by functional assay FACS or PCR, endotoxin, mycoplasma, and USP sterility testing. (cincinnatichildrens.org)
Knockdown of Akt was achieved with siRNA and stable transfection of expression vectors was performed. (jcancer.org)
Cells25
Broadly defined, transfection is the process of artificially introducing nucleic acids ( DNA or RNA ) into cells , utilizing means other than viral infection. (newworldencyclopedia.org)
Commonly, the term transfection is used in the sense of non-viral introductions of nucleic acid specifically into eukaryotic cells, or even more specifically into animal cells. (newworldencyclopedia.org)
Transfection of animal cells typically involves opening transient pores or "holes" in the cell plasma membrane , to allow the uptake of material. (newworldencyclopedia.org)
Because the term transformation had another sense in animal cell biology (a genetic change allowing long-term propagation in culture, or acquisition of properties typical of cancer cells), the term transfection acquired, for animal cells, its present meaning of a change in cell properties caused by introduction of nucleic acids by non-viral methods. (newworldencyclopedia.org)
1990) state that the meaning of transfection is "clear and unambiguous" and refers to the "process by which nucleic acid (DNA or RNA) is introduced to cells with the intent to be expressed in the recipient. (newworldencyclopedia.org)
The transfection process is different from "infection," which is the term used for a viral method of introducing nucleic acids into cells (Promega). (newworldencyclopedia.org)
Since the DNA introduced in the transfection process is usually not inserted into the nuclear genome, the foreign DNA is lost at the later stage when the cells undergo mitosis . (newworldencyclopedia.org)
If it is desired that the transfected gene actually remains in the genome of the cell and its daughter cells, a stable transfection must occur. (newworldencyclopedia.org)
However, efficient delivery of siRNA to target cells is being limited by lack of an effective delivery system that ensures efficient transfection into cells while protecting the encapsulated siRNA from nuclease. (mcw.edu)
These results demonstrate that the novel hybrid nanoparticles could potentially serve as a platform for efficient delivery of siRNA to cells for stable gene knockdown. (mcw.edu)
Transfection is the process of deliberately introducing naked or purified nucleic acids into eukaryotic cells. (wikipedia.org)
In animal cells, transfection is the preferred term as transformation is also used to refer to progression to a cancerous state (carcinogenesis) in these cells. (wikipedia.org)
Transfection can result in unexpected morphologies and abnormalities in target cells. (wikipedia.org)
The original meaning of transfection was "infection by transformation", i.e., introduction of genetic material, DNA or RNA, from a prokaryote-infecting virus or bacteriophage into cells, resulting in an infection. (wikipedia.org)
For work with bacterial and archaeal cells transfection retains its original meaning as a special case of transformation. (wikipedia.org)
Because the term transformation had another sense in animal cell biology (a genetic change allowing long-term propagation in culture, or acquisition of properties typical of cancer cells), the term transfection acquired, for animal cells, its present meaning of a change in cell properties caused by introduction of DNA. (wikipedia.org)
BFP expression in A549-Cas9-Blast stable cells infected with arrayed CRISPR lenti-gRNA viruses, seven days post infection, 20X magnification. (sigmaaldrich.com)
This method can be employed if a detectable marker is expressed on the cells post-transfection. (mirusbio.com)
Calcium phosphate transfection is a commonly used method for the introduction of DNA into eukaryotic cells. (sigmaaldrich.com)
Expression of ACP-ADRβ2 can be achieved by transiently transfecting cells in culture with standard transfection protocols. (neb.com)
Expression of the fusion protein cloned in pSNAP-CaaX can be achieved by transiently transfecting cells in culture with standard transfection protocols. (neb.com)
In transfection, the introduced nucleic acid may be transient, such that it does not replicate, or it may be stable and integrate into the genome of the recipient and replicate when the host genome replicates. (newworldencyclopedia.org)
This combines genome-wide expression profiling after RNA interference and stable transfection in order to detect pathways associated with these genes. (europa.eu)
Efficiencies1
Very high transfection efficiencies of up to 25% sporozoites in transient transfection and up to 9% oocysts following a single round of in vivo selection were achieved. (ox.ac.uk)
Luciferase expression was compared at 24 hours post-transfection using a standard luciferase assay. (bioscience.co.uk)
Efficiency1
A T7E1 mismatch detection assay was used to measure cleavage efficiency at 48 hours post-transfection. (bioscience.co.uk)
Genetic1
Successful development of methods to generate and isolate stable populations of transfected Eimeria parasites will now stimulate rapid expansion of reverse genetic studies in this important coccidian. (ox.ac.uk)
Deposit their cargo1
In addition to electroporation, transfection can be carried out using calcium phosphate, or by mixing a cationic lipid with the material to produce liposomes, which fuse with the cell plasma membrane and deposit their cargo inside. (newworldencyclopedia.org)
Plasmids1
Additionally, rKLK10 injection or ultrasound-mediated transfection of Klk10-expressing plasmids inhibited atherosclerosis in Apoe(-/-) mice. (cdc.gov)
Viral2
Transfection also has been used to refer to infecting a cell specifically with viral nucleic acid that is either isolated from a eukaryote virus or from a bacteriophage . (newworldencyclopedia.org)
As an alternative to the knockout model, RNA interference was used to decrease UOX expression using stable transfection in a mouse hepatic cell line (ATCC, FL83B). (cdc.gov)
Viruses1
Biological transfection is typically mediated by viruses, utilizing the ability of a virus to inject its DNA inside a host cell. (wikipedia.org)
The development of commercially available transfection reagents for gene transfer applications has revolutionized the field of molecular biology and scientific research. (mdpi.com)
Calcium1
The reagents supplied in the Calcium Phosphate Transfection Kit (Product No. CAPHOS) are sterilized by 0.2 µM filter and aseptically filled. (sigmaaldrich.com)
Expression6
E-cadherin local dynamics were studied in mature junctions, that is, junctions engaged in adhesion for many hours, in which cadherin expression level is stable. (cellimagelibrary.org)
Stable Expression pACP-ADRβ2 can be transfected as described above for transient transfection or by other standard transfection methods. (neb.com)
It is possible to use pools of stable cell populations for initial cell labeling to test for the presence of ACP-tag expression. (neb.com)
In most instances, difficulties in expression can be resolved by altering the transfection protocol. (neb.com)
Stable F98 glioma cell lines with MCT4 overexpression, normal expression, and knockdown were generated. (hindawi.com)
Stable flow-induced expression of KLK10 inhibits endothelial inflammation and atherosclerosis. (cdc.gov)
Transient and stable vector transfection: Pitfalls, off-target effects, artifacts. (nih.gov)
Hours5
Images (32X) captured at 48 hours post-transfection. (bioscience.co.uk)
Images (63X) were captured at 24 hours post-transfection using a Nikon A1R confocal microscope. (bioscience.co.uk)
The amount of GAPDH or AHA1 mRNA was measured relative to 18s rRNA levels using qRT-PCR and then normalized to the mRNA levels of the non-targeting control, 48 hours post-transfection. (bioscience.co.uk)
In most cases, ACP-ADRβ2 can be observed within 24 hours post-transfection. (neb.com)
Twenty four to 48 hours after transfection, begin selecting mammalian cultures in 600-1,200 µg/ml G418 (geneticin) depending on the cell line. (neb.com)
Populations1
Strategies to generate and stabilise populations of transfected Eimeria tenella are described here, together with the identification of optimal parameters for the transfection process. (ox.ac.uk)
These processes include transient transfection, and the creation of stable pools and clonal cell lines. (lonza.com)
High2
Other physical methods use different means to poke holes in the cell membrane: Sonoporation uses high-intensity ultrasound (attributed mainly to the cavitation of gas bubbles interacting with nearby cell membranes), optical transfection uses a highly focused laser to form a ~1 µm diameter hole. (wikipedia.org)
The application of recombinant DNA technology to fields such as drug discovery and development has led to an increased need for high-throughput transfection. (qiagen.com)
Selection2
Stable transfection of Eimeria species has been difficult to achieve because of the obligate requirement for in vivo amplification and selection of the parasites. (ox.ac.uk)
After 8-12 days of continuous selection, stable colonies will become visible. (neb.com)
Cell types1
This technique has been used to obtain both transient 1 and stable 2 transfections in a wide variety of cell types. (sigmaaldrich.com)
Transient and stable pool processes are generally used for discovery through early-stage development to enable rapid production and screening of large numbers of protein candidates, while stable clonal cell lines are used for GMP clinical and commercial manufacturing. (lonza.com)