• Neural plasticity secondary to carpal tunnel syndrome: a pseudo-continuous arterial spin labeling study. (iasp-pain.org)
  • To overcome the problem with excessive examination time for diagnosis of cerebral perfusion and/or the use of contrast agent to determine penumbra, we adopted a new magnetic resonance imaging technique named Acute Stroke Assessment using rapid Pseudo-continuous arterial spin labeling (ASAP-ASL) method. (minervamedica.it)
  • The signal to noise ratio (SNR) and acquisition time were compared with various numbers of signal average (NSA) of rapid pseudo-continuous arterial spin labeling (pCASL) using the 10-mm thick slice width and narrow scan range focusing the level of basal ganglia by healthy volunteers. (minervamedica.it)
  • Off-resonance correction for pseudo-continuous arterial spin labeling using the optimized encoding scheme. (ox.ac.uk)
  • Pseudo-continuous arterial spin labeling (PCASL) MRI has become a popular tool for non-invasive perfusion imaging and angiography. (ox.ac.uk)
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  • Pseudo-continuous arterial spinning labeling (pCASL) as an efficient non-invasive neuroimaging technique can be applied to directly quantify the neuronal activities of individual brain regions that show the persistent symptoms owing to its better spatial resolution and increased signal-to-noise ratio. (iasp-pain.org)
  • More recently, however, a hybrid approach, known as pulsed continuous or pseudocontinuous ASL (pcASL), has become popular, in which many short-duration RF pulses can be used to effectively label arterial water over a longer duration. (radiologykey.com)
  • ASLtbx is a MATLAB and Statistical Parametric Mapping (SPM12) based software used for processing Arterial Spin Labeled (ASL) data acquired with either the pulsed ASL (PASL), continuous ASL (CASL), or the pseudo-CASL technique. (va.gov)
  • Arterial spin labeling (ASL) magnetic resonance imaging (MRI) is unique in being a completely non-invasive method for imaging perfusion in the brain. (ox.ac.uk)
  • Arterial spin labeling (ASL) is a noncontrast perfusion imaging method that relies on the magnetic labeling of arterial water. (radiologykey.com)
  • The aim of my main research is to develop and validate MRI pulse sequences and post-processing tools for assessment of perfusion-related parameters using gd-based contrast agents (Dynamic Susceptibility Contrast MRI - DSC-MRI) and non-invasive methods (Arterial Spin Labelling - ASL and Intra Voxel Incoherent Motion - IVIM). (lu.se)
  • A micromagnet on top of the device provides a magnetic field gradient enabling electric-dipole spin resonance 19 and separates the resonance frequencies of the qubits in the presence of an external magnetic field (~320 mT) to 11.993 GHz (Q 1 ) and 11.890 GHz (Q 2 ). (nature.com)
  • Spin labels are molecules containing sites with unpaired electrons, usually stable nitroxide free radicals, and generate a paramagnetic center that can be probed using magnetic resonance spectroscopy. (glenresearch.com)
  • The site-directed spin labeling (SDSL) technique allows one to monitor a specific region within a protein. (wikipedia.org)
  • As shown in Figure 1, we offer two new nitroxide spin labels, TEMPO Azide (1) and TEMPO-TEG Azide (2), compatible with Click Chemistry for site directed spin labelling (SDSL). (glenresearch.com)
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  • Spin labels are normally used as tools for probing proteins or biological membrane-local dynamics using electron paramagnetic resonance spectroscopy. (wikipedia.org)
  • The goal of spin labeling is somewhat similar to that of isotopic substitution in NMR spectroscopy. (wikipedia.org)
  • Electron Paramagnetic Resonance (EPR) spectroscopy is a sensitive technique for the characterization of materials and has been used to determine the molecular environment of spin labels 1,2 , including the location, orientation and motion of spin labels within biomolecules. (glenresearch.com)
  • Consequently, spin labels localized within biomolecules can act as bioorthogonal chemical reporters for studying the structure and dynamics of biomolecules using EPR spectroscopy. (glenresearch.com)
  • We hope that the addition of these new spin labels, along with 5-Ethynyl-dU, will prove valuable to the growing field of EPR spectroscopy. (glenresearch.com)
  • A spin label (SL) is an organic molecule which possesses an unpaired electron, usually on a nitrogen atom, and the ability to bind to another molecule. (wikipedia.org)
  • Pushing the two-qubit gate fidelity well beyond 99% requires not only low charge-noise levels and the elimination of nuclear spins by isotopic enrichment but also careful Hamiltonian engineering. (nature.com)
  • This method combines the high labeling efficiency of CASL with the reduced hardware demands of PASL. (radiologykey.com)
  • Transfer the supernatant to a clean, labeled tube and store the DNA sample at 4°C until PCR amplification. (cdc.gov)
  • 2. Aliquot 1ml portions of supernatant into label ed cryovials. (who.int)
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  • We have undertaken electron paramagnetic resonance and spin trapping investigations of the photochemistry of kynurenine (KN), a natural component of the human eye and close analog of the principal chromophore in the young human lens 3-OH-kynurenine O-glucoside (3HKG). (nih.gov)
  • It defines the time duration for spins to travel from the labeling plane or volume to the imaged slices and is typically between 1500 and 2000 ms. Because ischemic stroke is defined by reduced flow via proximal routes, any remaining flow to the tissue is often supplied via collateral routes, which leads to increased delays between the labeling of the spins and the arrival of those spins in the imaged voxel. (radiologykey.com)
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  • There one replaces an atom lacking a nuclear spin (and so is NMR-silent) with an isotope having a spin I ≠ 0 (and so is NMR-active). (wikipedia.org)
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  • Here, we solved this problem and present the molecular design of a dual label (DL) compound comprising a highly fluorescent dye together with an EPR spin probe, which also renders the fluorescence lifetime to be concentration sensitive. (fu-berlin.de)
  • Regardless of the labeling approach, images are then acquired with and without labeling after allowing time for the labeled blood to pass from the site of labeling into the tissue of interest. (radiologykey.com)
  • Images are acquired without (control) and with (label) application of arterial inversion at the site of the labeling plane. (radiologykey.com)
  • The wide variety of alkyne modifications already available from Glen Research offers a diverse set of options for site-specific conjugation of spin labels to oligonucleotides. (glenresearch.com)
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  • 9. Using a swirling motion, remove the pel et with a pipette and transfer to label ed cryovial. (who.int)
  • Recently, spin-labelling has also been used to probe chemical local environment in NMR itself, in a technique known as Paramagnetic Relaxation Enhancement (PRE). (wikipedia.org)
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  • We have used these spin labels with 5-ethynyl-dU, our Copper-Free DBCO click labels, our Oligo Click Kits, and using copper sulfate mediated Click Chemistry. (glenresearch.com)
  • After the addition of biotinylated T3 and streptavidin-coated microparticles, the still-free binding sites of the labeled antibody become occupied, with formation of an antibody-hapten complex. (cdc.gov)
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  • although longer label times lead to a larger buildup of labeled spins in the voxel, the increased time may be better spent on acquiring additional pairs of label and control images for signal averaging. (radiologykey.com)
  • Sears Holdings Corp. says it will spin of the Lands' End brand amid continued financial struggles. (today.com)
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  • 32 P-labeled DNA probes were made from 2 µg PCR amplicons (purified by Montage PCR Cleanup Spin Column (Milipore Corp., Burlington, MA, USA). (cdc.gov)
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  • Proton-density images are acquired after the labeling and the PLD time. (radiologykey.com)
  • However, it suffers from sensitivity to off-resonance effects within the labeling plane, which can be exacerbated at high field or in the presence of metallic implants, leading to spatially varying signal loss and cerebral blood flow underestimation. (ox.ac.uk)
  • In this work we propose a prospective correction technique based on the optimized encoding scheme, which allows the rapid calculation of transverse gradient blips and RF phase modulations that best cancel phase offsets due to off-resonance at the locations of the feeding arteries within the labeling plane. (ox.ac.uk)
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  • 1. Transfer blood from the original tube to a label ed 50ml tube. (who.int)
  • 3. Transfer to a label ed 15ml tube and centrifuge at 2500xg for 10 minutes at 4°C. (who.int)
  • 1. Transfer the remaining blood from the plasma spin to a label ed 50ml tube containing 10ml RPMI 1640. (who.int)
  • Select and label enough unused 1.5 ml siliconized tubes to perform the extractions. (cdc.gov)
  • Spin 5 minutes at 14,000 × g at room temperature in a centrifuge designed to hold 1.5 ml tubes. (cdc.gov)
  • 6. Spin both tubes at 1200xg for 10 minutes. (who.int)
  • Their combination in a multiplexed approach promises a successful but ambitious strategy because of spin label-mediated fluorescence quenching. (fu-berlin.de)
  • She tells SPIN, "I'm only thinking about music right now. (spin.com)
  • Will Roberts is the primary lyricist for Spinning Lucy, and I have already developed much respect for the man. (blogspot.com)
  • Here we report a spin-based quantum processor in silicon with single-qubit and two-qubit gate fidelities, all of which are above 99.5%, extracted from gate-set tomography. (nature.com)
  • Reaching two-qubit gate fidelities above 99% has been a long-standing major goal for semiconductor spin qubits. (nature.com)
  • The sample is incubated with anti-TPO antibodies labeled with a ruthenium complexa. (cdc.gov)
  • After the addition of biotinylated TPO and streptavidin-coated microparticles, the anti-TPO antibodies in the sample compete with the ruthenium-labeled anti-TPO antibodies for the biotinylated TPO antigen. (cdc.gov)
  • 50 μL of sample, a biotinylated monoclonal TSH-specific antibody and a monoclonal TSH-specific antibody labeled with a ruthenium complex react to form a sandwich complex. (cdc.gov)
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  • Arterial spin labeling (ASL) is an emerging MRI technique for non-invasive measurement of cerebral blood flow (CBF). (edu.au)
  • In this schematic, the inverted blood in the label image is shown in blue. (radiologykey.com)
  • The label image has slightly less signal intensity due to the infusion of inverted magnetization provided by the inflowing labeled blood. (radiologykey.com)
  • 1. Spin vacutainer (about 9ml) at 815xg for 10 minutes at 4°C to separate plasma from blood cel s. (who.int)
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