Fluorescence microscopyAberrationsMicroscopeSpectroscopyPhoton-tunnelingPinholeConventionalPhotonicsAberration correctionDeconvolutionVivoExperimentallyContrastRefractiveNanoparticlesModalitiesFiber opticTomographyImmersion opticsCornealDarkfieldWavefrontImagingBiologicalAngiographyCorrectionExcitationRetinalMeasurementLensesAdaptive optics systemDetectorsElectronWavelengthsResolutionSurfaceLightEquivalentLensObjectivesCliniciansLaserSubjectsTechniquesPhysicsSystems
Fluorescence microscopy5
- Organic dyes: chemical structures and exploitation in fluorescence microscopy. (sns.it)
- It is an imaging technique similar to fluorescence microscopy which is able to detect absorbing nanoparticles (NPs) of sizes down to 1nm and even single absorbing molecules. (jimdofree.com)
- Conventional 3D laser scanning fluorescence microscopy requires repeated optical sectioning to create z-stacks in depth. (nature.com)
- Confocal fluorescence microscopy images of phospholipid bilayers supported by gallium phosphide nanowires. (lu.se)
- A combination of fluorescence microscopy, neutron reflectometry, and time-of-flight secondary-ion mass spectrometry data will be presented which characterizes the structure and composition of this new supported lipid bilayer category. (lu.se)
Aberrations14
- Simulation of specimen-induced aberrations for objects with spherical and cylindrical symmetry. (ox.ac.uk)
- Wavefront aberrations caused by the refractive index structure of the specimen are known to compromise signal intensity and three-dimensional resolution in confocal and multiphoton microscopy. (ox.ac.uk)
- However, adaptive optics can measure and correct specimen-induced aberrations. (ox.ac.uk)
- In this paper we have modelled specimen-induced aberrations caused by spherical and cylindrical objects using a ray tracing method. (ox.ac.uk)
- Aberrations for a simple model of an oocyte cell consisting of two spherical regions and for a model of a well-characterized optical fibre are calculated. (ox.ac.uk)
- However, tissue penetration can still be an issue due to scattering and spherical aberrations induced on focused beams by the tissue. (unimib.it)
- If the main lens is underfocused, it partially compensates for spherical aberrations and details in a -narrow range of sizes can be seen. (laserfocusworld.com)
- The results suggest that the MAL optimization can correct for eye aberrations with a pupil of 5mm or sample induced aberrations in microscopy. (spie.org)
- Since spherical aberrations are the dominating aberrations in confocal microscopy, it is not required to employ all degrees of freedom commonly offered by deformable mirrors. (spie.org)
- The operation at fixed trajectories in terms of focal length and spherical aberrations is demonstrated and investigated in terms of reproducibility. (spie.org)
- With conventional optical imaging, the major factor limiting the achievable resolution is the eye's monochromatic aberrations, which are due to imperfections in the optics of the eye. (touchophthalmology.com)
- Ophthalmic adaptive optics systems are designed to measure and correct for these higher-order aberrations, and can provide image resolution that is limited only by the pupil diameter of the eye, the axial length of the eye, and the wavelength of light. (touchophthalmology.com)
- As shown in Figure 1, ophthalmic adaptive optics imaging systems have three main components-a wavefront sensor (typically a Shack-Hartmann design, for measuring the eye's aberrations), a corrective element (typically a deformable mirror, for correcting the aberrations), and an imaging device (typically a charge-coupled device [CCD] or photomultiplier tube). (touchophthalmology.com)
- Nevertheless, the unifying feature of adaptive optics imaging systems is mitigation of the eye's aberrations to achieve nearly diffraction-limited imaging. (touchophthalmology.com)
Microscope16
- Among techniques that rely on the latter are those that improve the resolution only modestly (up to about a factor of two) beyond the diffraction-limit, such as confocal microscopy with closed pinhole or aided by computational methods such as deconvolution or detector-based pixel reassignment (e.g. re-scan microscopy, pixel reassignment), the 4Pi microscope, and structured-illumination microscopy technologies such as SIM and SMI. (wikipedia.org)
- By 1978, the first theoretical ideas had been developed to break the Abbe limit, which called for using a 4Pi microscope as a confocal laser-scanning fluorescence microscope where the light is focused from all sides to a common focus that is used to scan the object by 'point-by-point' excitation combined with 'point-by-point' detection. (wikipedia.org)
- Near-field optical random mapping (NORM) microscopy is a method of optical near-field acquisition by a far-field microscope through the observation of nanoparticles' Brownian motion in an immersion liquid. (wikipedia.org)
- Up to now, the best quality in a 4Pi microscope has been reached in conjunction with STED microscopy in fixed cells and RESOLFT microscopy with switchable proteins in living cells. (wikipedia.org)
- Harvard researchers integrated an immersion metalens into a commercial scanning confocal microscope, achieving an imaging spatial resolution of approximately 200 nm. (photonics.com)
- By integrating the oil immersion metalens (NA = 1.1) into a commercial scanning confocal microscope, the team was able to achieve an imaging spatial resolution of approximately 200 nm. (photonics.com)
- Deconvolution is often suggested as a good alternative to the confocal microscope, as both techniques seek to minimize the effect of out of focus fluorescence on your final image. (olympus-lifescience.com)
- This is not strictly true because images acquired using a pinhole aperture in a confocal microscope benefit from deconvolution processing. (olympus-lifescience.com)
- These tools can even be combined to reduce the noise in images acquired on a confocal microscope. (olympus-lifescience.com)
- 1 Steve Pennycook and other researchers incorporated advanced electron optics into a 300-kV Z-contrast (so-called because the return-electron intensity depends on the atomic number Z of the element being probed) scanning--transmission electron microscope (STEM), which allowed them to image silicon with a resolution of 0.6 Å (see figure). (laserfocusworld.com)
- The larger aperture also results in a smaller depth of field, which leads to the possibility of using the microscope to image planes at different depths - effectively making an electron-microscope equivalent of a visual confocal microscope. (laserfocusworld.com)
- P. Pankajakshan, Z. Kam, A. Dieterlen, and J.-C. Olivo-Marin, Characterizing the 3-D field distortions in low numerical aperture fluorescence zooming microscope, Optics Express Vol. 20, No. 9, Apr. (univ-mlv.fr)
- The optics of the eye can be thought of as an imperfect microscope objective through which the retina can be observed. (stanford.edu)
- The invention relates to a microscope (1), in particular an ophthalmic surgical microscope having a vitreoretinal viewing system (6), an optics carrier (14) and a support (2). (justia.com)
- To avoid contact of the front piece (30) with an eye (16) while the optics carrier (14) is moved for focusing a microscope lens (18), the position of the front piece (30) is automatically maintained stationary with respect to the support (2). (justia.com)
- Confocal - an interferometric microscope utilizing a rotating aperture and a CCD image analysis. (optekusa.com)
Spectroscopy2
- Furthermore, high-resolution optics are central to the field of spectroscopy, where they enable scientists to analyze the properties of materials and molecules at the atomic and molecular levels. (avantierinc.com)
- In the fluorescence area, specific projects involve the use of advanced fluorescence spectroscopy and microscopy for the analysis of protein adsorption on surfaces, petroleum fluids, biomedical polymers, and fluorophore photophysics. (universityofgalway.ie)
Photon-tunneling1
- Super-resolution imaging techniques rely on the near-field (photon-tunneling microscopy as well as those that use the Pendry Superlens and near field scanning optical microscopy) or on the far-field. (wikipedia.org)
Pinhole2
- Confocal microscopy prevents out of focus light from being detected by placing a pinhole aperture between the objective and the detector, through which only in focus light rays can pass. (olympus-lifescience.com)
- Early work on multi-modal 3D optical imaging is based on confocal laser scanning microscopy (LSM), where a confocal pinhole is applied for the depth sectioning and a stack of transverse images are taken for 3D reconstruction 1 . (nature.com)
Conventional4
- Part I: Conventional microscopy. (microscopyu.com)
- We determined whether neuroretinal rim assessment based on Bruch's membrane opening (BMO), rather than conventional optic disc margin (DM)-based assessment or retinal nerve fiber layer (RNFL) thickness, yielded higher diagnostic accuracy in myopic patients with glaucoma. (nih.gov)
- Non-linear excitation microscopy offers several advantages for in-vivo imaging compared to conventional confocal techniques. (unimib.it)
- This results in the best outcome that can be achieved by conventional microscopy. (lidsen.com)
Photonics2
- Product portfolio consists of a full range of solutions including motion control, optical tables and vibration isolation systems, photonics instruments, optics, and optomechanical components. (photonics.com)
- Optica Publishing Group developed the Optics and Photonics Topics to help organize its diverse content more accurately by topic area. (optica.org)
Aberration correction3
- Deformable mirrors are the standard adaptive optical elements for aberration correction in confocal microscopy. (spie.org)
- In this contribution, we present an alternative approach for aberration correction in confocal microscopy based on a novel adaptive lens with two degrees of freedom. (spie.org)
- Using digital holography, we characterize the tuning range of the focal length and the spherical aberration correction ability of the adaptive lens. (spie.org)
Deconvolution4
- Confocal microscopy: set-up, point spread function, hints on deconvolution, comparison with TIRF and 2-photon microscopy. (sns.it)
- 1 - Blind Deconvolution for Confocal Laser Scanning Microscopy . (inria.fr)
- Confocal microscopy is especially well suited for examining thick specimens such as embryos or tussues, while widefield deconvolution processing has proven to be a powerful tool for imaging specimens requiring extremely low light levels. (olympus-lifescience.com)
- P. Pankajakshan , G. Engler, L. Blanc-F raud and J. Zerubia, Deconvolution and denoising for confocal microscopy, in: Modeling in Computational Biology and Biomedicine: current trends (Ed. F.Cazals and P. Kornprobst) to be published in LN in Mathematical and Computational Biology by Springer, 2012. (univ-mlv.fr)
Vivo4
- These results are opening the way to the application of implanted micro-optics for optical in-vivo inspection of biological processes. (unimib.it)
- Swept-source optical coherence tomography (OCT), OCT angiography, and in vivo corneal confocal microscopy were performed in both groups. (arvojournals.org)
- In vivo confocal microscopy (IVCM) has been shown to reveal early corneal sub-basal nerve fiber changes in patients with diabetes before any apparent funduscopic alterations. (arvojournals.org)
- We will show that the Multi-actuator Adaptive Lens has been successfully used for in-vivo OCT ophthalmic imaging in both mice and humans, as well as confocal and two photon microscopy. (spie.org)
Experimentally1
- The spatial distribution of light emitted from optically pumped QD microdisk lasers with a single Si spherical nanoantenna placed on the top surface of the microdisk was studied experimentally by confocal optical microscopy. (hse.ru)
Contrast1
- Recently, with improving resolution, the technique of Z-contrast microscopy has gained popularity and it has been incorporated into commercial microscopes. (laserfocusworld.com)
Refractive1
- The metalenses can be easily adapted to focus light through multilayers of different refractive indices and mass-produced using modern industrial manufacturing or nanoimprint techniques, leading to cost-effective high-end optics. (photonics.com)
Nanoparticles1
- chemokines: B. Tsai, " Laser-Treated Substrate with Nanoparticles for Surface-Enhanced Raman Scattering, " Optics Letters, vol. (skiclub-todtmoos.de)
Modalities1
- The different modalities of super-resolution microscopy are increasingly being adopted by the biomedical research community, and these techniques are becoming indispensable tools to understanding biological function at the molecular level. (wikipedia.org)
Fiber optic3
- Designs and manufactures optical fiber, fiber optic cable, and specialty optical fiber solutions. (photonics.com)
- These unique capabilities are enabled by the Optics11 Life patented micro-machined fiber-optic sensors. (optics11life.com)
- Because the sensors are fiber-optic the instrument can measure both in-air and liquid. (optics11life.com)
Tomography2
- Participants underwent imaging with confocal scanning laser tomography for measurement of DM rim area (DM-RA) and with spectral domain optical coherence tomography (SD OCT) for quantification of a BMO-based neuroretinal rim parameter, minimum rim width (BMO-MRW), and RNFL thickness. (nih.gov)
- In this paper, we present a method termed oblique scanning laser microscopy (OSLM) to combine optical coherence tomography (OCT), for simultaneously volumetric structural and molecular imaging with cellular resolution in all three dimensions. (nature.com)
Immersion optics1
- They can be mass-produced with existing foundry technology or nanoimprinting for cost-effective high-end immersion optics. (photonics.com)
Corneal2
- Recently, ophthalmologists are able to qualify and quantify the retinal capillary network and choriocapillaries, optic nerve head, and corneal peripheral nerves. (arvojournals.org)
- An optic section of a keratoconic cornea shows corneal thinning. (medscape.com)
Darkfield1
- Using this in combination with a live feedback monitoring setup (using a carefully calibrated and precision-adjusted darkfield microscopy and tracking setup equipped with a multiple APD feedback circuit to lock-on onto a particle in focus) allows for full 3D navigation in a liquid. (jimdofree.com)
Wavefront1
- Our tests show that the MAL can be used both in combination with a wavefront sensor in a "classical" adaptive optics closed loop, or in a wavefront sensorless configuration. (spie.org)
Imaging8
- In digital microscopy , the main source of noise is either the signal itself (often referred to as photon shot noise) or the digital imaging system. (olympus-lifescience.com)
- ZEISS Celldiscoverer 7 is an automated live cell imaging system designed to make complex microscopy simple enabling high-throughput applications, but what about the data? (zeiss.co.uk)
- Optics Balzers provides innovative optical coatings and components, focusing on high-tech markets such as sensors and imaging, biophotonics, space and defense, lighting and projection, and industrial applications. (photonics.com)
- A combination of coherence gating and structured illumination allows a 3D multimodal imaging under a wide field microscopy with only incoherent sources 7 . (nature.com)
- The latter has allowed us to design more compact and simple imaging systems for different microscopy platforms. (spie.org)
- An infinite corrected objective lens with three screwed holes is assembled and mounted in a microscopy imaging system for testing. (avantierinc.com)
- This minimizes spherical aberration and allows for deeper imaging while maintaining high resolution performance. (nikon.com)
- Before getting into the clinical utility of adaptive optics imaging technology, it is prudent to first review the basic principles of imaging with adaptive optics. (touchophthalmology.com)
Biological2
- There are two major groups of methods for super-resolution microscopy in the far-field that can improve the resolution by a much larger factor: Deterministic super-resolution: the most commonly used emitters in biological microscopy, fluorophores, show a nonlinear response to excitation, which can be exploited to enhance resolution. (wikipedia.org)
- Confocal laser scanning microscopy is a powerful technique for studying biological specimens in three dimensions (3D) by optical sectioning. (inria.fr)
Angiography1
- Invited Session V: The eye as a window to systemic and neurodegenerative health: Seeking Answers through a keyhole: Harnessing the Synergy of Dynamic OCT/OCT Angiography and Adaptive Optics SLO for Retinal Assessment of Systemic Disease. (stanford.edu)
Correction1
- Our lab develops and uses adaptive optics, eye movement correction and optical microscopy technologies to improve the non-invasive visualization of the retina to the point that individual retinal structure and function and can be visualized at the cellular and even sub-cellular scale. (stanford.edu)
Excitation2
- Magnified fluorescence images can be retrieved through the microlenses coupled to commercial confocal and two-photon excitation scanning microscopes. (unimib.it)
- By fiber-optics, using one fiber for both excitation and fluorescence detection, the triplet and photo-radical state kinetics of locally applied MB and IR700 could then be monitored in a tissue sample. (bvsalud.org)
Retinal1
- En face OCT reflectance images which accompany OCTA studies offer a glimpse of the macrophage-like cellular activity above the retinal surface which responds to systemically instigated vascular events below. (stanford.edu)
Measurement1
- Provides user-oriented interferometers, transmission spheres, measurement systems, and software applications for precise measurement and testing of optics. (photonics.com)
Lenses3
- The use of adaptive lenses instead of deformable mirrors can simplify the implementation of an adaptive optics system. (spie.org)
- First results indicate that such adaptive lenses are a promising approach towards high-resolution, high-speed three-dimensional microscopy. (spie.org)
- Rigid contact lenses or scleral lenses (mainstay of vision therapy): Patients with early keratoconus may successfully use spectacles or spherical/toric soft contact lenses. (medscape.com)
Adaptive optics system1
- For the design of an adaptive optics system, information on the type and amount of the aberration is required. (ox.ac.uk)
Detectors1
- It gets more and more important due to recent developments in laser technology, more sensitive detectors, robust spectrometer optics, and, last but not least, through its easy use. (raman.de)
Electron2
- We combined serial block-face electron microscopy with light microscopy to determine the cell types targeted by HVC (RA) neurons, which control song timing. (elifesciences.org)
- A technique called 3D electron microscopy allowed the connections between the neurons in the clock brain region to be seen directly. (elifesciences.org)
Wavelengths1
- The team demonstrated liquid immersion metalenses free of spherical aberration at various design wavelengths in the visible spectrum . (photonics.com)
Resolution5
- Super-resolution microscopy is a series of techniques in optical microscopy that allow such images to have resolutions higher than those imposed by the diffraction limit, which is due to the diffraction of light. (wikipedia.org)
- High resolution and long working distance objective plays a crucial role in microscopy systems for medical device, scientific research, and industrial applications. (avantierinc.com)
- In the realm of optical microscopy, high resolution is paramount. (avantierinc.com)
- High-resolution microscopy has been instrumental in advancing medical research, enabling the diagnosis of diseases at earlier stages and facilitating breakthroughs in drug development. (avantierinc.com)
- Adaptive optics-based technologies offer non-invasive, cellular resolution views of the living retina, and bring with them several exciting clinical applications. (touchophthalmology.com)
Surface3
- The Chiaro nanoindenter uses this optical fiber sensor to gently push a spherical glass tip on the surface of the sample. (optics11life.com)
- The speed of a liquid lens is in general limited, because the liquid surface cannot follow fast voltage changes, while providing a spherical surface. (spie.org)
- Tsai, " Surface-enhanced Raman idleness first-order encountered by simple contract, " Optics Letters, vol. (skiclub-todtmoos.de)
Light3
- While polarized light from the sun is used as a compass ( 10 ), the information provided from optic flow (wide-field visual motion) allows bees to maintain a steady flight heading, altitude, and speed, and importantly it functions as a visual odometer with the integration of image velocity over time ( 11 ). (frontiersin.org)
- and a spectrometer configured to process the light signal collected by the optics assembly so as to generate a Raman spectrum of the detected sample. (justia.com)
- The optics assembly includes a first optical element configured to move, during irradiation of the excited light onto the sample, so as to change a position of a light spot of the excited light on the sample. (justia.com)
Equivalent1
- Myopic subjects with refraction error greater than -2 diopters (D) (spherical equivalent) and typical myopic optic disc morphology, with and without glaucoma, were recruited from a glaucoma clinic and a local optometry practice. (nih.gov)
Lens1
- CAMBRIDGE, Mass., June 2, 2017 - A flat lens for immersion microscopy has been developed. (photonics.com)
Objectives2
- Long working distance objectives are a fundamental component in microscopy that play a pivotal role in a wide range of scientific and industrial applications. (avantierinc.com)
- In this article, we will explore the importance of long working distance objectives and their applications in microscopy. (avantierinc.com)
Clinicians2
- The final classification of myopic glaucoma or myopic control was based on consensus assessment by 3 clinicians of visual fields and optic disc photographs. (nih.gov)
- Leveraging the multimodal synergy of combining dynamic methods of OCT/OCTA, with Adaptive Optics SLO, clinicians have new opportunities to better appreciate some of the complexities of human disease. (stanford.edu)
Laser2
- La microscopie confocale balayage laser, est une technique puissante pour tudier les sp cimens biologiques en trois dimensions (3D) par sectionnement optique. (inria.fr)
- Xiao, " Fiber Ring Laser Interrogated Zeolite Coated SMS Structure for Trace Chemical Detection, " Optics Letters, vol. (skiclub-todtmoos.de)
Subjects1
- Confocal microscopy studies have demonstrated a reduction in the number of keratocytes and stromal lamellae in KC compared to normal subjects, the reduction being greater in more advanced cases of KC [ 15 ]. (biomedcentral.com)
Techniques1
- Some of the following information was gathered (with permission) from a chemistry blog's review of sub-diffraction microscopy techniques. (wikipedia.org)
Physics1
- These led me to study physics and optics, first at the Universidad de la República (Uruguay), and later at Imperial College London (UK) and the University or Rochester (USA). (stanford.edu)
Systems1
- These systems use advanced optics to create intricate patterns on microchips with nanometer-level precision. (avantierinc.com)