• meiosis
  • These cells exit the mitotic cycle and enter meiosis as spermatocytes, exhibiting an extended G 2 phase characterized by a significant increase in cell mass and robust transcription. (biologists.org)
  • Spermatocytes in ofs mutant males failed to execute the G 2 -M transition of meiosis or substantive post-meiotic differentiation and had a significant cell size defect. (biologists.org)
  • The amount of antibody detected at kinetochores depends upon the stage of the spermatocytes: prometaphase kinetochores in meiosis I stain brightly for dynein (Fig. 2A and Fig. B, arrowheads), whereas metaphase kinetochores are more dimly labeled (Fig. 2C and Fig. D, arrowheads). (nih.gov)
  • chromosomes
  • The pattern of staining in insect spermatocytes was slightly different in the two species, but in general all three anti-D-titin antibodies stained the same components: the chromosomes, prophase and telophase nuclear membranes, the spindle in general, along kinetochore and non-kinetochore microtubules, along apparent connections between partner half-bivalents during anaphase, and various cytoplasmic components, including the contractile ring. (biologists.org)
  • In crane-fly spermatocyte spindles, for example, there are elastic `tethers' between two arms of the separating anaphase chromosomes. (biologists.org)
  • antibody
  • To isolate these genomic sequences, we performed chromatin immunoprecipitation assays in rat spermatocytes using an antibody against SYCP3, a major structural component of the LEs of the SC. (unam.mx)
  • cells
  • This article describes a method to isolate subpopulations of adult germ cells, more specifically pachytene spermatocytes, using two successive gradients, without using an elutriation centrifuge, a specialized device not available in many laboratories. (semanticscholar.org)
  • known
  • The level of ofs expression in spermatocytes is much higher than for the known eIF4G ortholog (known as eIF-4G or eIF4G), suggesting that Ofs substitutes for this protein. (biologists.org)
  • structure
  • The fine structure of bivalents from golden hamster and house cricket spermatocytes has been studied with a whole mount surface-spreading method combined with negative staining. (rupress.org)