• compounds
  • Also provided are compounds, devices, and kits for modifying surfaces such as glass surfaces. (google.com)
  • More specifically the invention relates to the use of such peptide-based compounds used as targeting vectors that bind to receptors associated with. (google.com)
  • Compounds are evaluated for their binding to naturally occurring receptors, by employing the natural ligand conjugated to an enzyme donor fragment of β-galactosidase for competing with the sample compound for the natural acceptor binding site or in the absence of competition where the sample compound binds to an allosteric site. (google.com)
  • The technical field of this invention is the measuring of the binding propensity of compounds to natural receptors. (google.com)
  • A large number of drugs are involved with binding to receptor compounds, where a signal may be transduced across a membrane, or the effective concentration of the drug is modulated by binding to a receptor, or the binding of a receptor to its ligand may be modulated allosterically, or the like. (google.com)
  • The invention relates to the use of light absorbing compounds to mediate the destruction of unwanted cells or tissues or, other undesirable materials by irradiation. (google.com)
  • Specifically, the invention relates to the use of hydro-monobenzoporphyrin derivatives having absorption maxima in the range 670-780 nanometers to mediate the irradiation of materials to be destroyed, and to the use of these compounds conjugated to target-specific ligands, such receptor-specific ligands, or immunoglobulins or their immunospecific fragments, to focus the effects of the irradiation on particular targets. (google.com)
  • The advantages of radioiodination, still the most versatile radiolabeling strategy, and other labeled compounds comprising covalently attached radioisotopes are compared to the use of chelator-protein conjugates that are complexed with metallic radioisotopes. (mdpi.com)
  • wherein
  • 2. The method according to Claim 1 , wherein said polymeric cation substance is a polymeric quaternary ammonium compound having a nitrogen content of at least about two percent. (google.ca)
  • 5. The method according to Claim 1 , wherein said polymeric cation substance is incorporated in or on said solid phase immediately before performance of the assay process. (google.ca)
  • 6. The method according to Claim 1 , further comprising the step of combining the test sample and said capture reagent to form a reaction mixture, wherein said reaction mixture is contacted to said solid phase. (google.ca)
  • 7. The method according to Claim 1 , further comprising the addition of an ancillary specific binding member, wherein said ancillary specific binding member is capable of binding the analyte and is capable of binding a member selected from the group consisting of said indicator reagent or said capture reagent in an indirect assay. (google.ca)
  • contacting the tissue with a surgical fastener having a functionalized surface including a plurality of complementary reactive members of the specific binding pair, wherein upon contact of the reactive members attached to the tissue via the linker, with the complimentary reactive members on the surgical fastener, covalent bonds are formed between the reactive members and the complementary reactive members, thus adhering the surgical fastener to the tissue. (google.ca)
  • 2. A method according to claim 1 wherein the members of the specific binding pair bind to one another via a reaction selected from the group consisting of Huisgen cycloaddition reaction, a Diels-Alder reaction and a thiol-ene reaction. (google.ca)
  • 3. A method according to claim 2 wherein the members of the specific binding pair are alkynes and azides. (google.ca)
  • 5. A method according to claim 2 wherein the reaction involves a cyclooctyne reagent and an azide for [3+cycloaddition. (google.ca)
  • 6. A method according to claim 2 wherein the members of the specific binding pair are thiols and alkenes. (google.ca)
  • 7. A method according to claim 2 wherein the surgical fastener is provided with the complimentary reactive members of the specific binding pair by surface modification techniques selected from the group consisting of physical vapor deposition, chemical vapor deposition, plasma treatments, coupling treatments, acid hydrolysis and base hydrolysis. (google.ca)
  • 9. A method according to claim 8 wherein the members of the specific binding pair are dienes and alkenes. (google.ca)
  • 12. A method according to claim 1 wherein the reactive members are attached to components of the tissue via a ligand-receptor linkage. (google.ca)
  • 6. A method of claim 5 wherein the noncleavable link is an amide bond, a bond between a sulfhydryl group an a maleimide derivative, a bond between a thiol and an amino group or a bond between an aldehyde and an amino group. (google.com)
  • d) determining that the cell-free quality control moiety produced a detectable reaction, wherein the detectable reaction provides a measure of the assay performance. (google.com)
  • 3. The method of claim 1 wherein the cell-free quality control moiety is covalently attached to the microscope slide by first attaching reactive groups to the slide wherein the groups are subsequently reacted with at least one cell-free quality control moiety. (google.com)
  • 4. The method of claim 1, wherein the redox-sensitive gene is selected from the group consisting of those media the expression of expressing cytokines involved in initiating the immune response, chemoattractants that promote the migration of inflammatory cells to a point of injury, growth factors, and adhesion molecules. (google.es)
  • 5. The method of claim 1, wherein the substance is pyrrolidine dithiocarbamate, or its pharmaceutically acceptable salt. (google.es)
  • 9. The method of claim 1, wherein the gene mediates the expression of thrombin receptor. (google.es)
  • conjugates
  • Targeting substance-diagnostic/therapeutic agent conjugates joined by stabilized Schiff base or hydrazone linkages are disclosed. (google.com)
  • The claimed invention relates to targeting substance-diagnostic/therapeutic agent conjugates that are joined by improved Schiff base or hydrazone linkages that provide advantageous properties for in vivo imaging and therapy. (google.com)
  • complexes
  • The specific binding of [ 3 H]-dexamethasone to glucocoticoid receptor (GR) and activation of hormone-receptor (H-R) complexes from the liver of chicken at day 0, 5, 10, 30, 60 and 90 were investigated to find out GR regulation during postnatal development. (niscair.res.in)
  • In contrast, we have shown that CADY:siRNA complexes clearly induce a transient cell membrane permeabilization, which is rapidly restored by cell membrane fluidity. (jove.com)
  • Therefore, we propose that direct translocation is the major gate for cell entry of CADY:siRNA complexes. (jove.com)
  • Intravenous delivery of siRNA ITSN /cationic liposome complexes transiently knocked down ITSN-1s protein and mRNA in mouse lungs at day 3, which recovered after additional 3 days. (jove.com)
  • membrane
  • 5. An agent as claimed in claim 1 comprising gas microbubbles stabilised by a coalescence resistant surface membrane, a filmogenic protein, a polymer material, a non-polymeric and non-polymerisable wall-forming material or a surfactant. (patents.com)
  • Membrane perturbation and uptake are driven mainly by the ability of CADY to interact with phospholipids within the cell membrane, followed by rapid localization of the complex in the cytoplasm, without affecting cell integrity or viability. (jove.com)
  • comprises
  • 12. A pharmaceutical composition which is useful in targeting specific biological material which composition comprises an effective amount of the compound of claim 1 or 2 in admixture with at least one pharmaceutically acceptable excipient. (google.com)
  • peptide
  • We recently proposed a peptide-based nanoparticle system, CADY, for efficient delivery of siRNA into numerous cell lines. (jove.com)
  • A method for the treatment of cardiovascular diseases and noncardiovascular inflammatory diseases that are mediated by VCAM-1 is provided that includes the removal, decrease in the concentration of, or prevention of the formation of oxidized polyunsaturated fatty acids, or interferes with a complex formed between a polyunsaturated fatty acid or an oxidized polyunsaturated fatty acid and a protein or peptide that mediates the expression of VCAM-1. (google.es)
  • group
  • A group of hydro-monobenzoporphyrins 'green porphyrins' (Gp) having absorption maxima in the range of 670-780 nanometers is useful in treating disorders or conditions which are subject to hematoporphyrin derivative (HPD) treatment in the presence of light, or in treating virus, cells and tissues generally. (google.com)
  • 1. A method for suppressing the expression of a redox-sensitive gene selected from the group consisting of MCP-1, IL-6 and thrombin receptor comprising administering an effective amount of a substance that prevents or minimizes the oxidation of a polyunsaturated fatty acid. (google.es)
  • novel
  • Thus, the recent pressing challenge of microbial research is the identification of novel drug targets and the development of inhibitors that can be used for therapy of human infections. (aspetjournals.org)
  • modification
  • Surface modification plays an important role in micro-array biomolecule detection technology for controlling backgrounds and spot morphology. (google.com)
  • immune
  • Dendritic cells (DCs) can be considered sentinels of the immune system which play a critical role in its initiation and response to infection 1 . (jove.com)
  • This process is typically characterized by production of type 1 interferon along with other proinflammatory cytokines, upregulation of cell surface markers such as MHCII and CD86 and migration of the mature DC to draining lymph nodes, where interaction with T cells initiates the adaptive immune response 2,3 . (jove.com)
  • diagnosis
  • Endobronchial Ultrasound-guided Transbronchial Needle Aspiration (EBUS-TBNA) is a frequently used procedure for diagnosis and staging of non-small cell lung cancer (NSCLC). (jove.com)
  • biological
  • Liposomes performance and biological activity depend on their size, charge, lipid composition, stability, dose and route of administration 3 Here, efficient and specific KD ITSN in mouse lungs has been obtained using a cholesterol and dimethyl dioctadecyl ammonium bromide combination. (jove.com)
  • form
  • In one embodiment a test sample suspected of containing the analyte of interest may be contacted with the capture reagent to form a charged capture reagent/analyte complex. (google.ca)
  • sample
  • and d) detecting said indicator reagent associated with said solid phase to determine the presence or amount of the analyte in the test sample. (google.ca)
  • step
  • Attaching DNA to a modified glass surface is a central step for many applications in DNA diagnostics industry including gene expression analysis. (google.com)
  • drug
  • As a consequence these drugs allow developing patient-specific therapies that provide the means to go beyond the possibilities of current drug therapy. (mdpi.com)
  • site
  • The tracers used in molecular imaging require labeling techniques that provide site specific conjugation and metabolic stability. (mdpi.com)
  • delivery
  • Cell penetrating peptides constitute a potent approach to overcome the limitations of in vivo siRNA delivery. (jove.com)
  • Long-term Silencing of Intersectin-1s in Mouse Lungs by Repeated Delivery of a Specific siRNA via Cationic Liposomes. (jove.com)
  • different
  • The surface loadings always vary with different silanes and even same silane may not give reproducible results. (google.com)
  • Application
  • Appropriate choice of the radionuclide allows tailoring the properties of the labeled protein to the application required. (mdpi.com)
  • techniques
  • With the techniques using radioactive isotopes as a reporting unit or even the therapeutic principle, care has to be taken to avoid cleavage of the radionuclide from the protein it is linked to. (mdpi.com)
  • least
  • The use of a polycationic substance, having a nitrogen content of at least about two percent (excluding the counter ions), to prepare a suitably charged solid phase provides a solid phase that can be subjected to a greater extent of manipulation, such as multiple washes, without losing the capability to attract and retain the capture reagent. (google.ca)