OrganismsChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesAnthropology, Education, Sociology and Social PhenomenaInformation Science
Random Amplified Polymorphic DNA TechniqueDNA, RecombinantDNA FingerprintingMolecular Sequence DataBase SequenceCloning, MolecularEscherichia coliPlasmidsPublicationsPublication BiasPublishingPeriodicals as TopicReactive Nitrogen SpeciesInternetDuplicate Publication as Topic
RAPDPolymorphismMarkersPolymerase2000NucleotideAFLPStrainsAmplificationFragmentsGeneGenesSequencesSequenceMarkerPatternsFalciparumVarietiesProteinsGenomicPlantsRegionsEnzymeASSESSMENTPhylogeneticWidelyDiscriminateSpeciesHighlyPrimerMolecular techniquesInvolvesGenomeProductsAnalyticalDiseasesProteinDiagnosisMassCombinationIdentifyStudiesTemplate
RAPD13
  • Thus, the Random Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) technique was used to examine the genetic variability among individuals of S . olivacea from Setiu Wetlands, Terengganu. (mabjournal.com)
  • Twenty reproducible RAPD fragments generated by OPA 08 and OPA 10 with 17 fragments were polymorphic. (mabjournal.com)
  • Application of Random Amplified Polymorphic DNA (RAPD) in Molecular Ecology. (mabjournal.com)
  • A total of 78 RAPD primers were used and 8 presented differences in band patterns with 54% of the amplified polymorphic fragments. (scielo.br)
  • A caracterização molecular foi baseada em marcadores RAPD, sequenciamento da região do espaçador interno transcrito do DNA ribossomal e "fingerprinting" telomérico. (scielo.br)
  • Foi usado um total de 78 primers RAPD, sendo que 8 apresentaram 54% de fragmentos de DNA polimórficos. (scielo.br)
  • The genetic variability of the isolates was first assessed using Random Amplified Polymorphic DNA (RAPD) markers. (bvsalud.org)
  • RAPD (Random Amplified Polymorphic DNA) markers have been most widely used for this purpose 8-10 . (bvsalud.org)
  • have been studied by RAPD (random amplified polymorphic DNA) and statistical cluster analysis. (jipb.net)
  • The results indicate that the application of RAPD technique in the taxonomic study has gained some resolution which could not be obtained by morphological analysis and has provided available data for developing longan production and for breeding new longan cultivars. (jipb.net)
  • 1984). Solanum demissum, phic DNA (RAPD) and restriction fragment length poly- a Mexican hexaploid species, has been used extensively morphism (RFLP) markers that are closely linked to the in potato breeding programs. (health-abstracts.com)
  • The BC progenies RAPD markers were grouped into synteny groups and the synteny were obtained from crosses between three different somatic hy- groups assigned to chromosomes with chromosome-specific RFLP brids (J101, J103, and J138) and the S. tuberosum cultivars Katah- probes as described in McGrath et al. (health-abstracts.com)
  • Restriction Fragment Length Polymorphism (RFLP), Random Amplified Polymorphic DNA (RAPD), Amplified Fragment Length Polymorphism (AFLP), Single-Strand Conformation Polymorphism (SSCP) and Microsatellite DNA. (biomedcentral.com)
Polymorphism5
  • The percentage of polymorphic bands was 85% indicates that the population of S. olivacea has high level of polymorphism due to low inbreeding factor within a population. (mabjournal.com)
  • The special cuhivars, "Lidongben", "Nanhu Jiaohe" and "Lizhiben" were named respectively as Lidongben group, Nanhu Jiaohe group, and Lizhiben group, because of their more characteristic DNA polymorphism tested in all respective cuhivars. (jipb.net)
  • alata ) using amplified fragment length polymorphism (AFLP) technique. (open-science-repository.com)
  • Rapid molecular identification and typing methods that may be useful include 16S rRNA gene sequencing, random amplified polymorphic DNA and a combination polymerase chain reaction-restriction fragment length polymorphism analysis ( 39 , 116 ). (antimicrobe.org)
  • sessed, out of which 249 were polymorphic revealing 96.13% polymorphism. (scirp.org)
Markers11
  • Populations specific genetic markers and diseases. (mabjournal.com)
  • A number of markers are now available to detect nuclear DNA polymorphisms. (intechopen.com)
  • 4, 5 The process involves use of many evenly spaced polymorphic DNA markers to create landmarks across each chromosome. (bmj.com)
  • Specific molecular and biochemical markers associated with Fusarium wilt resistance were identified. (ppjonline.org)
  • The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases. (lookformedical.com)
  • This review gives a brief summary on the development of genetic markers including both the classical genetic markers and more advanced DNA-based molecular markers. (biomedcentral.com)
  • By the 1980s, many different types of DNA molecular markers had been explored, e.g. (biomedcentral.com)
  • All of these DNA-based markers contain specific advantages and have played significant roles in the evaluation of genetic diversity in farm animals. (biomedcentral.com)
  • Chromosomes are the carriers of genetic material and chromosome mutations are crucial sources of genetic variation [ 5 ], we can use these mutations as markers to determine the specific location of a gene on the chromosome, and its position relative to other genes. (biomedcentral.com)
  • Molecular techniques have facilitated the identification, genotyping, and monitoring of food contamination sources, identification of genetically modified organisms, and use as food detection methods to avoid falsification through genetic markers. (peertechzpublications.com)
  • Residual heterozygosity in the initial inbreds can partly explain the observed responses as evidenced by 42 markers derived from both Methyl-Sensitive Amplification- and Amplified Fragment Length- Polymorphisms. (biomedcentral.com)
Polymerase3
  • This has been done using techniques such as polymerase chain reaction, gene restriction, use of probe hybridization and also using primers. (australiaessays.com)
  • Its principle is based on the use of DNA polymerase which is an in vitro replication of specific DNA sequences. (intechopen.com)
  • DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. (lookformedical.com)
20001
  • 2000). The main advantage in this context is that the complex genetic and epigenetic correlations among interconnected parts of the brain can be explored using complementary molecular, developmental, structural, pharmacological, and behavioral techniques. (nervenet.org)
Nucleotide4
  • We can therefore amplify nucleotide sequences from infinitesimal amounts of DNA extract. (intechopen.com)
  • Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. (lookformedical.com)
  • An enzyme capable of hydrolyzing highly polymerized DNA by splitting phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide. (lookformedical.com)
  • It's been approximated that to anticipate the ancestry of people, up to thousand random one nucleotide polymorphisms (SNPs) or brief tandem repeats (STRs) may 864814-88-0 manufacture be required [13]. (tech-strategy.org)
AFLP1
Strains4
  • Effect of Development Stage on the Artemisinin Content and the Sequence Characterized Amplified Region (SCAR) Marker of High-Artemisinin Yielding Strains of Artemisia annua L. [J]. J Integr Plant Biol. (jipb.net)
  • With the release of a genome wide SNPs database, it has become feasible to analyse these genetically determined QTLs for genes polymorphic in these strains. (bmj.com)
  • To discriminate P. falciparum strains, the sizes of amplified products of these genes are compared, routinely based on migration patterns with agarose-gel electrophoresis because of ease of use and low cost. (biomedcentral.com)
  • The application of molecular techniques in food is large, several authors comment on the detection and quantification of strains of pathogenic microorganisms present in foods and the presence of toxins and waste allergens not related to specific goods in production lines. (peertechzpublications.com)
Amplification1
  • Indeed, if the sequence of interest is present in the DNA extract, it is possible to selectively replicate it (we speak of amplification) in very large numbers. (intechopen.com)
Fragments1
  • The monomorphic fragments of 600 bp (P. expansum) and 594 bp (P. griseoroseum) were amplified. (scielo.br)
Gene6
  • Genomics and changes associated with gene patterns have enabled the prediction of etiological factors leading to a disease being shed light on genetic mapping and prognosis on alterations of DNA in novel diseases. (australiaessays.com)
  • Next, the isolates were differentiated by sequencing a specific region of the gene encoding the enzyme glucosyltransferase B ( gtfB ). (bvsalud.org)
  • These species were validated primarily on the basis of DNA-DNA hybridization, 16S rRNA gene sequence analysis, antimicrobial susceptibility and biochemical profiles, and, to a lesser degree, on high-performance liquid chromatography ( 22 , 54 , 55 , 69 ). (antimicrobe.org)
  • Plant transformation techniques are well developed for making transgenic varieties in certain crops and model organisms, yet reagent delivery and plant regeneration remain key bottlenecks to applying the technology of gene editing to most crops. (frontiersin.org)
  • Some examples include delivering DNA-free gene editing reagents such as ribonucleoproteins or mRNA, relying on reagent expression from non-integrated DNA, using novel delivery mechanisms such as viruses or nanoparticles, using unconventional selection methods to avoid integration of transgenes, and/or avoiding tissue culture altogether. (frontiersin.org)
  • Gene editing tools are being applied in basic research to close the knowledge gap, but the requirements for gene editing techniques for agriculture are different than those for research. (frontiersin.org)
Genes4
  • Much of the resistance to resistance have been noted by analysis of three different late blight in this species is due to a series of race-specific backcross-2 populations derived from two different so- resistance (R) genes. (health-abstracts.com)
  • Both gel and capillary methods evaluated polymorphic regions of the merozoite surface protein 1 and 2 and glutamine rich protein genes. (biomedcentral.com)
  • Most commonly, genotyping methods take advantage of variation in highly polymorphic genes of Plasmodium falciparum , including merozoite surface proteins 1 and 2( msp1 and msp2 ) and glutamine-rich protein ( glurp ) [ 2 ]. (biomedcentral.com)
  • Cis-acting DNA sequences which can increase transcription of genes. (lookformedical.com)
Sequences1
  • In this case, the basic principle is to detect specific DNA and RNA sequences via hybridization of a probe, detecting any complementary sequence on the cDNA of this bacterium. (australiaessays.com)
Sequence5
  • These species share a high sequence homology of 16S rDNA and several molecular techniques already applied to discriminate among them give ambiguous results. (biomedcentral.com)
  • This method can generate tens of billions of copies of a particular DNA fragment (the sequence of interest, DNA of interest, or target DNA) from a DNA extract (DNA template). (intechopen.com)
  • A method for determining the sequence specificity of DNA-binding proteins. (lookformedical.com)
  • One could say the primary limitation to crop improvement is no longer, "can we make a precise sequence change in a precise location in eukaryotic genomic DNA? (frontiersin.org)
  • A high-quality reference sequence for the Brassica napus genome is not yet available, thus studies of specific regions of the Brassica napus genome at sequence level have relied on the analysis of bacterial artificial chromosomes (BACs) or BAC contigs. (biomedcentral.com)
Marker1
  • At present, DNA molecular marker techniques are widely applied in the fields of germplasm identification, phylogenetics, and genetic structural analysis. (biomedcentral.com)
Patterns1
  • Based on a combination of mitochondrial and nuclear DNA, the following questions were addressed: 1) the phylogeography of the two O. h. robertsoni phylogroups, 2) regional and local population structure in space and time, and 3) patterns of local dispersal under different isolation-by-distance scenarios. (biomedcentral.com)
Falciparum1
  • RDTs are point-of-care tests that detect circulating antigens, such as the P. falciparum -specific histidine rich protein 2 (PfHRP2) or histidine rich protein 3 (PfHRP3), as well as the pan- Plasmodium spp. (biomedcentral.com)
Varieties1
  • Random amplified polymorphic DNA has proven useful in sorting out the relationships of the C. pepo species, varieties, and cultivars, showing that few, if any, modern cultivars have their origins with C. texana. (wikipedia.org)
Proteins2
  • These diagnostic methods are at the cellular and molecular level since both DNA and proteins are major components of a cell. (australiaessays.com)
  • Proteins which bind to DNA. (lookformedical.com)
Genomic1
  • The genomic DNA was extracted from ten samples of mud crab by using DNeasy kit. (mabjournal.com)
Plants1
  • In a population of BC plants A potentially more durable race-non-specific re- line J10lK6 sistance is present in many of the diploid Mexican spe- [( S. tuberosum PI 203900+ S. bulbocastanum PI 243510) cies, notably S. bulbocastanum (Neiderhauser and Mills ×Katahdin)]×Atlantic, late blight resistance cosegregated 1953). (health-abstracts.com)
Regions1
  • Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. (lookformedical.com)
Enzyme2
  • DNA samples (10 µg) were digested with Eco Rl, Hin dlll, Eco RV, These three BC populations are designated 1K6, 1K27 and LB1, or Dra l at a DNA/enzyme ratio of 7 units per microgram. (health-abstracts.com)
  • The enzyme has a preference for double-stranded DNA. (lookformedical.com)
ASSESSMENT2
  • Mitochondrial DNA diversity of mud crab Scylla olivacea (Portunidae) in Peninsular Malaysia: a preliminary assessment. (mabjournal.com)
  • Provision of broad, method-based PT schemes, such as DNA sequencing, would allow assessment of a large number of tests for which formal PT is not currently available. (cdc.gov)
Phylogenetic1
  • Finally, the integration of both molecular cytogenetic and phylogenetic approaches allowed the determination of specific chromosomes possibly involved in rearrangements and a better understanding about the evolutionary processes involved in the differentiation of Synbranchus genus. (scielo.br)
Widely1
  • The developed PCR-RFLP technique resulted in efficient discrimination of the tested species and subspecies and allowed the construction of a dichotomous key in order to differentiate the most widely distributed Bifidobacterium species as well as the subspecies belonging to B. pseudolongum and B. animalis . (biomedcentral.com)
Discriminate1
  • In order to find those anatomical characters that statistically discriminate among higher basommatophoran taxa, we performed discriminant analyses (DA) with DNA lineages as a grouping variable. (malacsoc.org.uk)
Species3
  • The development of every species under its particular natural ecosystem, environmental, and socio-economic conditions has led to each having its own specific genetic characteristics. (biomedcentral.com)
  • Since Nocardia species infections are very often sporadic, information from randomized clinical trials comparing the clinical efficacy of specific antimicrobial agents is lacking. (antimicrobe.org)
  • Avian MHC organization is of particular interest because the chicken Gallus gallus , the avian species with the best characterized MHC, possesses a highly streamlined minimal essential MHC, which is linked to resistance against specific pathogens. (biomedcentral.com)
Highly1
  • Three primer combinations that produced clear scorable and highly polymorphic bands were screened from 64 Eco RI and Ms eI primer combinations. (open-science-repository.com)
Primer1
  • The use of different polymerases, DNA/primer ratios or different annealing temperatures may lead to a discrepancy in the results obtained in different laboratories[ 24 ]. (biomedcentral.com)
Molecular techniques3
  • In the last years several molecular techniques have been proposed in order to identify bifidobacteria. (biomedcentral.com)
  • This review aims to address the use of molecular techniques applied to the identification of food. (peertechzpublications.com)
  • Studies have been demonstrating the effectiveness of molecular techniques regarding the accuracy of the protocols used and the agility to provide the necessary answers. (peertechzpublications.com)
Involves1
  • A Feature Paper should be a substantial original Article that involves several techniques or approaches, provides an outlook for future research directions and describes possible research applications. (mdpi.com)
Genome1
  • In many crops, genome engineering techniques have enabled researchers and breeders to take advantage of a vast knowledge base of plant physiology, pathology, and genetics. (frontiersin.org)
Products2
  • Despite biocontrol conceptually being a useful way to control specific pests, there are very few products that are used beyond the glasshouse level, into the field. (weizmann.ac.il)
  • This catalyzes endonucleolytic cleavage of DNA yielding 5'-phosphodi- and oligonucleotide end-products. (lookformedical.com)
Analytical1
  • Immunoassay refers to a class of analytical methods based on antigen-antibody-specific interactions. (peertechzpublications.com)
Diseases1
  • The recent developments in proteomics particularly in the field of bioinformatics have given a new light to the diagnosis of specific diseases and also the formulation of the related cures. (australiaessays.com)
Protein1
  • Specific protein-binding measures are often used as assays in diagnostic assessments. (lookformedical.com)
Diagnosis2
  • Diagnosis of anal sphincter tears to prevent fecal incontinence: a random- ized controlled trial. (top5binarybrokers.com)
  • Making the diagnosis may often involve performing invasive techniques on the patient and may take up to 2 or 3 weeks. (antimicrobe.org)
Mass2
  • 1998). For convenience, the gel (Diversified Biotech, Boston, Mass.) 0.6% agarose gel, stained clones of S. bulbocastanum and S. tuberosum used in this study with ethidium bromide and visualized on a UV transilluminator. (health-abstracts.com)
  • Do not send non-specific, mass emails to everyone in the department hoping for a match. (ubc.ca)
Combination1
  • The parts of a macromolecule that directly participate in its specific combination with another molecule. (lookformedical.com)
Identify1
  • Identify specific faculty members who are conducting research in your specific area of interest. (ubc.ca)
Studies1
  • DISCUSSION: The identification of these specific environmental factors associated with ALS highlight the need for future prospective and laboratory studies to assess causality, biological mechanisms, and find prevention or treatment opportunities. (cdc.gov)
Template2