• molecular
  • In this study, we investigated the molecular basis for this exceptional biosynthetic capability by comparison of alkaloid profiles with gene expression profiles between 16 different Papaver species. (opioids.com)
  • HSPs primarily function as molecular chaperones to prevent aggregation and to promote appropriate refolding of denatured proteins caused by HS, which leads to increased thermotolerance ( Parsell and Lindquist, 1993 ). (plantphysiol.org)
  • By measuring shifts in the absorption spectrum, enhancement of α-parinaric acid fluorescence, induced circular dichroism, and energy transfer between tryptophan amino acids in the protein and the bound chromophore, information may be gleaned about the molecular interactions between protein and lipid. (wikipedia.org)
  • Arabidopsis thaliana is a first class model organism and the single most important species for fundamental research in plant molecular genetics. (wikipedia.org)
  • As molecular biology methods progressed, many investigators sought to focus community effort on common model plant species. (wikipedia.org)
  • Growing amounts of DNA sequence data facilitated development and application of such molecular markers. (wikipedia.org)
  • domain-containi
  • Carboxy-terminal to this, they possess an unstructured domain containing multiple SH2 binding site motifs, which when tyrosine-phosphorylated allow interaction with SH2 domain containing proteins. (wikipedia.org)
  • heat shock pr
  • M. tardigradum and H. dujardini protein clusters and conserved domains imply metabolic storage pathways for glycogen, glycolipids and specific secondary metabolism as well as stress response pathways (including heat shock proteins, bmh2, and specific repair pathways). (uni-regensburg.de)
  • It is under tight regulation by heat shock factors (HSFs) and heat shock proteins (HSPs) after heat shock (HS) to prevent stress damage. (plantphysiol.org)
  • HSR is universally invoked in all organisms and is characterized by elevated synthesis of a specialized set of proteins called heat shock proteins (HSPs). (plantphysiol.org)
  • abundant
  • With abundant sequence data of numerous plants in public databases such as NCBI and PlantGDB, the identification of their miRNAs and targets would benefit researchers as a central resource for the comparative studies of plant miRNAs. (biomedcentral.com)
  • For example, this technique is used to investigate how fatty acids bind to serum albumin (a highly abundant blood protein), lipid transport processes including structural characterization of lipoproteins, and phospholipid-transfer proteins. (wikipedia.org)
  • encodes
  • 1. A recombinant mammalian host cell containing a recombinant or synthetic nucleic acid comprising an open reading frame sequence corresponding to nucleotides 1 to 3315 of SEQ ID NO: 1 that encodes a polypeptide having an amino acid sequence of SEQ ID NO: 2 or an enzymatically active fragment thereof, said open reading frame contained in a human DNA insert of an ˜3.5 kb NotI-BstEII restriction fragment of plasmid pGRN109. (google.com)
  • MALSU1 is a gene on chromosome 7 in humans that encodes the protein MALSU1. (wikipedia.org)
  • The first and second each contain 7 exons and encode the same full-length protein isoform a (786 amino acids, considered the major isoform), the third one contains 6 exons and encodes a shorter isoform b (732 amino acids) and the fourth one contains 5 exons and encodes the shortest isoform c (349 amino acids). (wikipedia.org)
  • membrane
  • Hopefully, this work will facilitate future functional characterization of the anoctamin membrane protein family. (beds.ac.uk)
  • family proteins
  • For the better studied members of the CAS family (BCAR1 and NEDD9), all of these domains have been defined as crucial for recognition and binding by other proteins, reflecting the primary role of CAS family proteins as cell signaling cascades mediators. (wikipedia.org)
  • It also lacks a YDYVHL sequence at the N-terminal end of the FAT-like carboxy-terminal domain, even though this motif is conserved among the other three CAS family proteins and is an important binding site for the Src SH2 domain. (wikipedia.org)
  • organisms
  • In marine broadcast spawning organisms, behavioral and mechanical isolation are absent and prezygotic barriers between species often operate only during the fertilization process. (biomedcentral.com)
  • Therefore, behavioral and mechanical isolation are absent in broadcast spawning organisms and if two sympatric broadcast spawning species are not temporally isolated [ 22 ], prezygotic barriers between these two species occur only during the fertilization process. (biomedcentral.com)
  • This work paralleled the Human Genome Project and related projects for other model organisms, and built on efforts to sequence expressed sequence tags from A. thaliana. (wikipedia.org)
  • Despite these difficulties, extensive transcript and protein sequence databases have been generated for human as well as other important model organisms in biology, such as mice and yeast. (wikipedia.org)
  • in 2008 following in silico screening of databases describing expressed sequence tags from an evolutionarily diverse group of organisms, using the CAS-related proteins (p130Cas, NEDD9/HEF1 and EFS) mRNAs as templates. (wikipedia.org)
  • mutagenesis
  • Insertional mutagenesis is when transposons function as vectors to help remove and integrate genetic sequences. (wikipedia.org)
  • Signature-tagging mutagenesis (also known as STM) is a technique focused on using transposable element insertion to determine the phenotype of a locus in an organism's genome. (wikipedia.org)
  • The A. thaliana genome sequence, and low-cost Sanger sequencing, and ease of transformation facilated genome-wide mutagenesis, yielding collections of sequence-indexed transposon mutant and (especially) T-DNA mutant lines. (wikipedia.org)
  • genetic
  • They can be harnessed as a genetic tool for analysis of gene and protein function. (wikipedia.org)
  • Given their relatively simple design and inherent ability to move DNA sequences, transposons are highly compatible at transducing genetic material, making them ideal genetic tools. (wikipedia.org)
  • In the treatment of genetic diseases, the insertion of a transposon into deleterious gene locus of organism's genome would misalign the locus sequence, truncating any harmful proteins formed and rendering them non-functional. (wikipedia.org)
  • began their experiment by constructing a genetic sequence consisting of the Hmox-1 transposable element and transposase from Sleeping Beauty. (wikipedia.org)
  • A. thaliana was the first plant for which a high-quality reference genome sequence was determined (see below), and a worldwide research community has developed many other genetic resources and tools. (wikipedia.org)
  • Given a protein sequence, a family of possible coding DNA sequences can be derived by reverse translation of the genetic code. (wikipedia.org)
  • evolutionary
  • Our data and analysis suggest that evolutionary strategies in efficient aerobic cellular respiration are controlled by hemoglobin and mitochondrial proteins, which may be important for the adaptation of Antarctic fish to their environment. (biomedcentral.com)
  • We sequenced the genome of an Antarctic bullhead notothen, N. coriiceps , applying a whole genome shotgun approach to a total of 84.5× coverage for its estimated genome size of 637 Mb to understand these evolutionary mechanisms. (biomedcentral.com)
  • Because non-reproductive proteins don't feel the same evolutionary pressure as Acps, they aren't evolving nearly as quickly. (wikipedia.org)
  • Evolutionary divergence of the CAS proteins family members is discussed by Singh et al. (wikipedia.org)
  • predict
  • Using fluorescence 2-D difference gel electrophoresis to compare the proteomes of genotypes in an F2 population of S. graminum that segregated for virus transmission ability we recently discovered a panel of protein biomarkers that predict vector competency. (usda.gov)
  • This has also led us to predict the structural and putative functional motifs, moreover a number of critical amino acid sites that may be of importance for the functional divergence in the anoctamin protein family have been identified. (beds.ac.uk)
  • Predicting the function of a gene and confirming that the gene prediction is accurate still demands in vivo experimentation through gene knockout and other assays, although frontiers of bioinformatics research[citation needed] are making it increasingly possible to predict the function of a gene based on its sequence alone. (wikipedia.org)
  • divergence
  • Our results show that anoctamin protein paralogs evolved from several gene duplication events followed by functional divergence of vertebrate anoctamins. (beds.ac.uk)
  • In addition we identified a number of protein domains, and amino acid residues which contribute to predicted functional divergence. (beds.ac.uk)
  • Consistent with the arms race theory, DNA analyses reveal a two-fold increase in Acp divergence relative to non-reproductive proteins For many females, reproduction can be very dangerous and disadvantageous as in the case of bed bugs mentioned previously. (wikipedia.org)
  • Drosophila
  • Additionally, sexual antagonistic co-evolution can be the cause of rapid evolution, as is thought to be the case in seminal proteins known as Acps in species of Drosophila melanogaster. (wikipedia.org)
  • P elements are transposons originally described in Drosophila melanogaster genome capable of being artificially synthesized or spread to other Drosophila species through horizontal transfer. (wikipedia.org)
  • lipids
  • All three of these proteins are able to deliver lipids to acceptor membranes, but this transfer process in Ov-FAR-1 and ABA-1A1 (a type of NPA) requires an aqueous diffusion step. (wikipedia.org)
  • characteristics
  • Open reading frames from a further 39 novel sequences, without counterparts in the database, were screened for the characteristics of intrinsically disordered proteins, i.e. hydrophilicity and lack of stable secondary structure. (biologists.org)
  • α-Parinaric acid has been used to study the hydrophobicity and foaming characteristics of food proteins, as well as the foam stability of beer. (wikipedia.org)
  • All CAS protein family members have common structural characteristics. (wikipedia.org)
  • mRNAs
  • In 1979 teams at Harvard and Caltech extended the basic idea of making DNA copies of mRNAs in vitro to amplifying a library of such in bacterial plasmids In 1982, the idea of selecting random or semi-random clones from such a cDNA library for sequencing was explored by Greg Sutcliffe and coworkers. (wikipedia.org)
  • mitochondrial
  • This protein localizes to mitochondria and is probably involved in mitochondrial translation or the biogenesis of the large subunit of the mitochondrial ribosome. (wikipedia.org)
  • nematode
  • DUF1693 has been identified as part of the nucleotidyltransferase superfamily and contains four nematode prion-like proteins, but the exact function remains unknown. (wikipedia.org)
  • novel
  • During this project, Ames documented 9 novel Chaetomium species including the culture Ames described as C. cupreum which was sent to him by Paul Marsh of the U.S Department of Agriculture from deteriorating material collected in the Panama Canal Zone. (wikipedia.org)
  • enzymes
  • tested this notion by using Sleeping Beauty transposons to help insert sequences into mice with sickle cell anemia so they can produce the enzymes need to counteract their anemia. (wikipedia.org)
  • recombinant
  • Purified and recombinant proteins TPC2 and TPC3 and recombinant or synthetic oligonucleotides corresponding to those proteins or fragments thereof can be used to detect regulators of telomere length and telomerase activity in mammalian cells and for a variety of related diagnostic and therapeutic pu. (google.com)
  • mammals
  • Candidate gamete recognition proteins from two lineages of C. intestinalis (Type A and B) are evolving more rapidly than control proteins, consistent with patterns seen in insects and mammals. (biomedcentral.com)
  • relatively
  • The resulting sequence is a relatively low-quality fragment whose length is limited by current technology to approximately 500 to 800 nucleotides. (wikipedia.org)
  • Once candidate DNA sequences have been determined, it is a relatively straightforward algorithmic problem to efficiently search a target genome for matches, complete or partial, and exact or inexact. (wikipedia.org)
  • known
  • Enhanced prezygotic isolation in sympatry has become a well-known feature of gamete recognition proteins in marine broadcast spawners. (biomedcentral.com)
  • Monocarboxylate transporter 2 (MCT2) also known as solute carrier family 16 member 7 (SLC16A7) is a protein that in humans is encoded by the SLC16A7 gene. (wikipedia.org)
  • Transport and golgi organization 2 homolog (TANGO2) also known as chromosome 22 open reading frame 25 (C22orf25) is a protein that in humans is encoded by the TANGO2 gene. (wikipedia.org)
  • It is characterized by the NRDE superfamily domain (DUF883), which is strictly known for the conserved amino acid sequence of (N)-Asparagine (R)-Arginine (D)-Aspartic Acid (E)-Glutamic Acid. (wikipedia.org)
  • This domain is found among distantly related species from the six kingdoms: Eubacteria, Archaebacteria, Protista, Fungi, Plantae, and Animalia and is known to be involved in Golgi organization and protein secretion. (wikipedia.org)
  • Palmitoylation is known to play a role in the modulation of proteins' trafficking, stability and sorting. (wikipedia.org)
  • Chaetomium cupreum is known only as a sexually reproducing species and no asexual form has been reported. (wikipedia.org)
  • libraries
  • This makes it difficult to write programs that can unambiguously determine that two EST libraries were sequenced from the same tissue. (wikipedia.org)