Species injection sperm tail. Medical search. Frequent questions

Removal of sperm tail using trypsin and pre-activation of oocyte facilitates intracytoplasmic sperm injection in mice and rats. (stanford.edu)
We examined various methods to enhance the accessibility of intracytoplasmic sperm injection (ICSI) technology to more users by making the technique easier, more efficient, and practical. (stanford.edu)
Creating a viable donkey embryo has been challenging, but UQ's Dr Andres Gambini, in collaboration with Argentinian and Spanish researchers, made it happen using a specialist IVF process known as intracytoplasmic sperm injection (ICSI). (edu.au)
Intracytoplasmic sperm injection ( ICSI / ˈ ɪ k s i / IK -see ) is an in vitro fertilization (IVF) procedure in which a single sperm cell is injected directly into the cytoplasm of an egg . (wikipedia.org)
Schematic image of intracytoplasmic sperm injection in the context of IVF . (wikipedia.org)
or (2) the in vitro fertilization technology known as intracytoplasmic sperm injection (ICSI), in which a technician uses a pipette to mechanically insert a sperm into an ovum. (robertpgeorge.com)

Shug human trials showed a 97% decrease in sperm motility through the vas deferens. (wikipedia.org)
Moreover, the distinction between living round spermatids, to be used in ROSI, and dead round spermatids, to be discarded, needs specific methods and skills, not required in the case of ICSI where sperm cell viability can be easily evaluated on the basis of sperm motility in most cases. (wikipedia.org)
Functional assessments on sperm included counting sperm amount and sperm motility, staining F-actin, and quantifying adenosine triphosphate (ATP) content. (biomedcentral.com)
Testicular torsion-detorsion significantly decreased the amount of sperm, inhibited the motility, declined the F-actin expression, and reduced the content of ATP in sperm. (biomedcentral.com)
Local injection of MSCs improved sperm function, particularly in sperm motility. (biomedcentral.com)
Local injection of MSCs into a testis damaged by testicular torsion-detorsion restores sperm function mainly through the improvement of sperm motility and energy. (biomedcentral.com)
ROS plays a very important role in the regulation of sperm motility, capacitation, and acrosome reaction. (carrottopng.com)
Sperm motility is essential for fertilization, and ROS plays a role in regulating the movement of sperm cells. (carrottopng.com)
Studies have shown that ROS can affect the motility of sperm cells, either positively or negatively. (carrottopng.com)
Low levels of ROS can enhance sperm motility, while high levels can lead to decreased motility and even immobilization. (carrottopng.com)
Oxidative stress can lead to reduced sperm count, motility, and morphology, as well as increased sperm DNA fragmentation. (carrottopng.com)
In addition to their regulatory roles in sperm motility, capacitation, and acrosome reaction, ROS also act as signalling molecules. (carrottopng.com)

In terms of insemination, ICSI needs only one sperm cell per oocyte , while IVF needs 50,000-100,000. (wikipedia.org)
This condition is called non-obstructive or secretory azoospermia, as opposed to obstructive azoospermia, in which complete sperm production does occur in the testicles, and potentially fertilizing spermatozoa can be obtained by testicular sperm extraction (TESE) and used for ICSI. (wikipedia.org)
[2] The microinjection procedure for ROSI also differs slightly from that of ICSI, since additional stimuli are needed to ensure proper oocyte activation after spermatid injection. (wikipedia.org)
On the other hand, when the sperm is mechanically inserted into the ovum via ICSI, it is not clear precisely when the ovum and the sperm unite and cease to be, or at least, it is not clear to us - and we have not taken a position on the question. (robertpgeorge.com)

Sperm cells are highly sensitive to reactive oxygen species (ROS), which are produced during cellular oxidation. (mdpi.com)
The cytoplasmic droplet then becomes known as excess residual cytoplasm, which can impair overall sperm function and produce higher levels of reactive oxygen species, potentially leading to male infertility. (biomedcentral.com)
What are Reactive Oxygen Species? (carrottopng.com)
Reactive oxygen species (ROS) are chemically active molecules that contain oxygen which the body produces as a byproduct of metabolism. (carrottopng.com)
Reactive oxygen species (ROS) are important for the development and maturation of sperm cells in the male reproductive system. (carrottopng.com)

In normal cell biology, ROS levels increase with a decreasing antioxidant response, resulting in oxidative stress which threatens sperm biology. (mdpi.com)
Oxidative stress has numerous effects, including increased apoptosis, reduced motion parameters, and reduced sperm integrity. (mdpi.com)
However, excessive ROS levels can lead to oxidative stress, which can damage sperm DNA and impair male fertility. (carrottopng.com)

Three days after the surgery, orchiectomy was executed and the testis, epididymis, and sperm were separated to each other. (biomedcentral.com)
MSCs significantly reversed the imbalance of glycolysis in sperm and testis induced by testicular torsion-detorsion, as evidenced by increasing the expression of phosphoglycerate kinase 2 and glyceraldehyde-3-phosphate dehydrogenase-spermatogenic, activating Akt, and increasing glycogen synthase kinase 3 (GSK3), which led to the increase in glycolysis cascades and ATP production. (biomedcentral.com)

It can be used in teratozoospermia , because once the egg is fertilized, abnormal sperm morphology does not appear to influence blastocyst development or blastocyst morphology. (wikipedia.org)
[6] Even with severe teratozoospermia, microscopy can still detect the few sperm cells that have a "normal" morphology, allowing for optimal success rate. (wikipedia.org)

and in human subjects, a urethane-coated nylon IVT resulted in significantly reduced sperm count but could not guarantee absolute sterility at the same rate as a vasectomy. (wikipedia.org)

This is because the acrosome reaction has to take place and thousands of sperm cells have to be involved in IVF. (wikipedia.org)
The acrosome reaction is a process where the acrosome, a specialized structure in the sperm head, undergoes exocytosis, allowing the sperm cell to penetrate the egg's outer layer. (carrottopng.com)
ROS help initiate the acrosome reaction, which is essential for sperm to penetrate the egg's outer layer. (carrottopng.com)

The exact mechanism of the deactivation effect on sperm is still unknown. (wikipedia.org)
Serotonin, a potent neuromodulator and tryptophan metabolite, had no effect on sperm swim speeds or on orientation. (biologists.com)

Currently, women undergoing IVF must take a series of extremely expensive hormone injections to stimulate their ovaries to mature eggs, which can cause cramping, bloating, headaches, mood disruptions, and painful ovarian swelling. (bostonglobe.com)
In this review, we summarize the results regarding the activation of primordial oocytes in neonatal and adult ovaries of different species and propose a model in which ovaries of neonatal mammals contain a mixed population of both quiescent and activated primordial oocytes, while almost all primordial oocytes are quiescent in adult females. (go.jp)

Or even same-sex couples to create sperm from biological females or eggs from biological males and conceive children who are genetically related to both parents. (bostonglobe.com)
This procedure is most commonly used to overcome male infertility problems, although it may also be used where eggs cannot easily be penetrated by sperm, and occasionally in addition to sperm donation . (wikipedia.org)
Such cytoskeletal changes appeared to render the eggs more receptive to sperm, as judged by the significant alleviation of polyspermy by latrunculin-A and mycalolide-B. In addition, our fluorimetric assay provided the first evidence that nicotine directly accelerates polymerization kinetics of G-actin and attenuates depolymerization of preassembled F-actin. (mdpi.com)
Third, many amphibian species lay vast numbers of eggs, providing adequate numbers for study. (biomedcentral.com)
In the case of consort males, who left spermatophores inside the female's body, their sperm springs into action as soon as the female's eggs start oozing out of her oviducts. (science20.com)
But in the case of sneaker males, their sperm doesn't get to touch the eggs until they're already outside her body, having passed by the consort's sperm. (science20.com)
This investigation considered the behavioral responses of red abalone ( Haliotis rufescens ) sperm to soluble factors released into sea water by conspecific eggs. (biologists.com)
Sperm in proximity to individual live eggs swam significantly faster and oriented towards the egg surface. (biologists.com)
Eggs released L-tryptophan at concentrations that triggered both activation and chemotaxis in sperm, exhibiting significant activity at levels as low as 10 -8 mol l -1 . (biologists.com)
In experimental treatments involving an elevated, uniform concentration of tryptophan (10 -7 mol l -1 ) or the addition of tryptophanase, an enzyme that selectively digests tryptophan,sperm failed to navigate towards live eggs. (biologists.com)
A natural gradient of L-tryptophan was therefore necessary and sufficient to promote recruitment of sperm to the surface of eggs in red abalone. (biologists.com)

In the male reproductive system, ROS plays a vital role in the development and maturation of sperm cells. (carrottopng.com)

RISUG is composed of styrene maleic anhydride (SMA) dissolved in dimethyl sulfoxide (dimethyl sulfoxide), which is believed to both partially occlude the vas deferens by coating the inner walls of the vas deferens with the polymer chemical, while also deactivating the sperm that are able to pass through the partially occluded vas deferens, thereby preventing successful fertilization. (wikipedia.org)
Round spermatid injection (ROSI) is a technique of assisted reproduction whereby a round spermatid is injected into oocyte cytoplasm in order to achieve fertilization. (wikipedia.org)
Although males of other species employ various mating tactics, when it comes to actual fertilization, it always happens in the same place, either internal (for some species) or external (for other species). (science20.com)
Since the inside of the female's body is a radically different environment than outside in the open ocean, they suspect that it must be better to have small sperm for internal fertilization and large sperm for external fertilization. (science20.com)
Mating likely occurs in late summer or autumn and the sperm is stored in the female's reproductive tract until spring when ovulation and fertilization occurs. (tripod.com)
Mating usually occurs in Autumn, and the sperm is stored within the female until spring, when fertilization occurs. (tripod.com)
Fertilization in humans and other mammals produces a new member of the species in the embryonic stage of its natural development. (robertpgeorge.com)
At fertilization, the ovum and the sperm cease to be and something new comes to be - an organism (the embryo) whose genetic constitution and epigenetic state orient and dispose it to develop in the direction of maturity as a member of the species. (robertpgeorge.com)
In normal (natural or standard in vitro ) fertilization, many sperm penetrate the corona radiata of the ovum (a layer of follicle cells surrounding the ovum). (robertpgeorge.com)
It seems reasonable to hold that if the sperm can be retrieved and still behave as a sperm, without the assistance of extensive manipulation, fertilization has not yet occurred. (robertpgeorge.com)
Dissolved signal molecules that cause sperm to orient and accelerate towards an egg could play pivotal roles in fertilization success,but such compounds are largely undescribed. (biologists.com)

Proof of pregnancy: vaginal plug or sperm in a vaginal lavage. (europa.eu)

Vas-occlusive contraception is a form of male contraception that blocks sperm transport in the vas deferens, the tubes that carry sperm from the epididymis to the ejaculatory ducts. (wikipedia.org)
Human sperm cell is a flagellate cell consisting of a disc-shaped head and a long tail released from the seminiferous tubule and mature in the epididymis. (biomedcentral.com)

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In cases of nonobstructive (secretory) azoospermia, on the other hand, testicular sperm production is blocked at different stages of the process of sperm formation (spermatogenesis). (wikipedia.org)
We previously reported that mesenchymal stem cells (MSCs), through local injection upon testicular torsion-detorsion, restored the spermatogenesis without differentiation into sperm. (biomedcentral.com)
Human stem cell factor contributed the activation of Akt/GSK3 axis when sperm suffered from testicular torsion-detorsion-induced germ cell injury. (biomedcentral.com)
Duration of testicular torsion and the severity of cord twisting are two key prognostic factors for sperm survival and activity. (biomedcentral.com)
We previously explored that local injection of mesenchymal stem cells (MSCs) 30 min before surgical detorsion was applicable to prevent testicular torsion-induced I/R injury on germ cell and maintain the spermatogenesis at acute stage. (biomedcentral.com)

Then, typically, only one sperm will penetrate the zona pellucida (a film of glycoproteins surrounding the oocyte) and reach the oocyte. (robertpgeorge.com)

It is a familiar element of mammalian spermatozoa, though many studies in the past have focused primarily on CDs in non-human species. (biomedcentral.com)
Immunohistochemistry using two sets of antibodies, one that recognized full-length PRL and N-terminal cleaved PRL and another that recognized full-length PRL and C-terminal cleaved PRL, suggested that intact PRL was localized in the nucleus of round spermatids, while N-terminal cleaved PRL variants were localized in the Golgi apparatus of the sperrmatid nuclei of round spermatids, cytoplasms of elongated spermatids and in the spermatozoa tails. (go.jp)

MPVs were previously considered distinct from CDs and characteristic of immature sperm [ 13 ]. (biomedcentral.com)

this allows cytoplasmic factors derived from the ovum to affect the nuclear contents derived from the sperm - for example, new types of histones begin to be associated with those chromosomes, modifying the behavior and interaction of the molecules in these chromosomes. (robertpgeorge.com)

however, the use of activated oocytes resulted in the same fecundity as that of fresh, untreated sperm. (stanford.edu)
Pre-activated oocytes were more resistant to physical damage, showed higher survival rates, and required less time per injection. (stanford.edu)
If all requirements for round spermatid selection and injection are successfully met, the injected oocytes develop to early embryos and can be transferred to the mother's uterus to produce pregnancy. (wikipedia.org)

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Sneaker males make bigger sperm. (science20.com)

Reversible inhibition of sperm under guidance (RISUG) is a non-toxic polymer gel-based contraceptive that is injected into the lumen of the vas deferens and results in infertility within 10 days following injection. (wikipedia.org)
Or enable men with infertility problems to generate healthy sperm from other kinds of cells. (bostonglobe.com)
Thus, MSCs may potentially rescue torsion-detorsion-induced infertility via local injection. (biomedcentral.com)

Chemically mediated behavior may thus be a key component regulating sperm-egg interactions, whether occurring in the turbulent ocean environment or within a mammalian reproductive tract. (biologists.com)

This chemical combination resulted in a 96% azoospermia and a 99% pregnancy prevention eight years follow injection in humans. (wikipedia.org)

The CDs of mature non-human mammalian sperm are found at the distal end of the midpiece. (biomedcentral.com)

Unlike the gametes - the sperm and egg cells independent of each other - it is no mere part of another organism, nor is it merely something that can be used to produce a complete organism. (robertpgeorge.com)

Ectopic expression of gene BCL2 enhances the survival and proliferation of chimpanzee and pig-tailed macaque iPSCs within the pre-implantation embryo, although the identity and long-term contribution of the transplanted cells warrants further investigation. (stanford.edu)
A University of Queensland-led research group has created the world's first successful donkey embryo using in-vitro fertilisation (IVF), which could save dozens of endangered donkey species. (edu.au)
The result would not be a sperm and an ovum, but only nuclear material from a zygote, on the one hand, and a disabled embryo (or perhaps the death of the embryo), on the other. (robertpgeorge.com)

Considerable effort has thus been devoted to generating induced pluripotent stem cells (iPSCs) from multiple non-human primate species. (stanford.edu)
More successful IVF, egg cells from men, and sperm cells from women? (bostonglobe.com)
The sperm cells undergo biochemical and physiological changes that enable them to fertilize an egg. (carrottopng.com)
These toxins can damage sperm cells and lead to reduced fertility. (carrottopng.com)

Injection of medical polyurethane (MPU) to form a plug in the vas deferens resulted in azoospermia in 96% of men, though these result were seen 24 months after injection. (wikipedia.org)

Like all bats , the Eastern Small-Footed Bat has a flight membrane that connects the body to the forelimbs and tail, which creates the bats flight. (tripod.com)

Insertion of an intravasal thread (IVT), also known as an intravasal device (IVD), in the vas deferens has been shown to inhibit sperm transport via mechanical obstruction and the removal of the IVT results in restored vas deferens patency. (wikipedia.org)

The injection of the chemical combination of ethanol and formaldehyde also resulted in sterility in humans. (wikipedia.org)
Chemical communication between sperm and egg is a key factor mediating sexual reproduction. (biologists.com)
Chemical characterization by nuclear magnetic resonance spectroscopy indicated that the free amino acid L-tryptophan was the natural sperm attractant in H. rufescens . (biologists.com)

At this point the genetic material from the ovum (the female pronucleus) and the genetic material from the sperm (the male pronucleus) are both contained within a single new cell, are being moved toward each other, and will eventually intermingle. (robertpgeorge.com)

As a unique functional test of these iPSCs, we injected them into the pre-implantation embryos of another non-human species, rhesus macaques (Macaca mulatta). (stanford.edu)
This is the point just after the fusion of the membranes of the sperm and the ovum, when the ovum and the sperm cease to be, and a new organism - a whole human organism - comes to be. (robertpgeorge.com)

Hermo and coworkers [ 8 ] conducted studies on rat CDs, and in these species, the droplet comprises of lamellae and small vesicles unlike structural components of the Golgi apparatus and endoplasmic reticulum. (biomedcentral.com)

Genetic studies in this organism are not practical because of the tetraploid nature of the genome and the long generation time of this species. (biomedcentral.com)
Two features of X. laevis that have hindered genetic studies are the long generation time (1 to 2 years) and the tetraploid genome of this species. (biomedcentral.com)

But we have no solid evidence for what is the threshold, if any, for the sperm count to exceed in order to allow the male to be fertile [4,5,7]. (infertile.com)

Trypsin treatment was found to efficiently remove the sperm tails. (stanford.edu)
Northerns are often found roosting singly in caves, rather than in the large clusters typical of other Myotis species, like Myotis sodalis . (tripod.com)
The researchers found that humanity could, using this method, cut our species' annual contributions to the greenhouse effect in half at a price that states and large cities spend all the time on highways, subways and other infrastructure projects: a total of about $3.5 billion over the course of the next 15 years to develop the technology. (livescience.com)
A region essential for this Golgi retention was also found in the C-terminal tail of P/rds and supported the cilia targeting of P/rds mediated by unconventional secretion. (jneurosci.org)

Studies revealed that ROS takes part in the activation of signalling pathways that are critical for the fusion of sperm and egg. (carrottopng.com)

But if she is, the female porcupine will curve her tail over her back so that the male won't be impaled when he mounts her. (livescience.com)
That's because squids (and octopuses and all other cephalopods) package their sperm up before passing it from male to female. (science20.com)

Resembling a small sperm whale, it has a personality worthy of the Herman Melville novel. (listverse.com)
The Eastern Small-footed Bat is a species of vesper bat in the Vespertilionidae family. (tripod.com)

For this reason, CDs are not considered detrimental to proper sperm function. (biomedcentral.com)

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Although some muscles obtain well-defined motor endplates, other muscles may insist a more staid spassume from of injection across the muscle 54 or higher dilutions. (upb.ro)

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Anatomy16

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Phenomena and Processes14

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Intracytoplasmic sperm inj6

Motility12

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Testis2

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Acrosome3

Effect on sperm2

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