• This was inferred from sequence analysis of rrs (16S rRNA) and other genetic loci and suggests that they belong to a novel species. (nih.gov)
  • The NEBNext rRNA Depletion Kit (bacteria) removes the abundant bacterial rRNAs (5S, 16S, and 23S) resulting in the enrichment of bacterial mRNAs and non-coding RNAs of interest. (genomeweb.com)
  • Ribosomal RNA (rRNA) is highly abundant in bacterial samples, and its removal is desirable in order to reveal the biological significance of less abundant transcripts. (neb.com)
  • Specific enrichment of bacterial mRNAs is challenging due to their lack of poly(A) tails, and so the converse - efficient and specific removal of bacterial rRNA - is necessary. (neb.com)
  • The NEBNext ® rRNA Depletion Kit (Bacteria) employs the NEBNext RNase H-based RNA depletion workflow to target removal of rRNA from gram-positive and gram-negative organisms. (neb.com)
  • The great majority of RNA in bacteria is ribosomal RNA (rRNA). (neb.com)
  • Ribosomal RNA was depleted using the NEBNext rRNA Depletion Kit (Bacteria). (neb.com)
  • Effective depletion of sequences overlapping with annotated rRNA regions was observed at 100 ng and 10 ng of input RNA for most of the organisms. (neb.com)
  • Recent advances in high-throughput deep sequencing of the bacterial 16S ribosomal RNA (rRNA) has enabled researchers to analyse the skin microbiome. (cosmeticsdesign-europe.com)
  • Methods Stools were analysed by PCR-DGGE for assessment of the total bacterial and fungal communities by analysis of 16S rRNA and 28S rRNA, respectively. (bmj.com)
  • The 16S ribosomal RNA (16S rRNA) gene is present in most bacterial species. (technologynetworks.com)
  • For bacteria, the assays target the 16S rRNA gene, and for fungi, they target ribosomal RNA genes. (qiagen.com)
  • Our new NEBNext Ultra II RNA kits have streamlined, automatable workflows and are available for directional (strand-specific, using the "dUTP method") and non-directional library prep and are compatible with poly(A) mRNA enrichment or rRNA depletion. (neb.com)
  • For ribosomal RNA (rRNA) depletion from human, mouse, or rat samples, use the NEBNext rRNA Depletion Kit (Human/Mouse/Rat) ( NEB #E6310 ). (neb.com)
  • For depletion of bacterial rRNA from bacterial samples, use the NEBNext rRNA Depletion Kit (Bacteria) ( NEB #E7850 ). (neb.com)
  • Many commercial kits will reduce host and or bacterial rRNA sequences, and use a variety of approaches. (usda.gov)
  • DNA probes, complementary to primarily to rRNA and select mitochondrial sequences host sequence, can be used to degrade host RNA using RNAse H treatment. (usda.gov)
  • The same approach can be used to target bacterial rRNA sequences. (usda.gov)
  • This approach can also leave part of the 16s rRNA bacterial sequences untargeted to allow for their use of identification of important bacterial pathogens. (usda.gov)
  • Because bacterial rRNA sequences are more variable among the different phylum, additional iterative approach to deplete bacterial sequences will be used. (usda.gov)
  • The remaining genes provide instructions for making molecules called transfer RNA (tRNA) and ribosomal RNA (rRNA), which are chemical cousins of DNA. (medlineplus.gov)
  • Some of the enzymes which generate aromatic radicals that break down most prominent bacterial strains found were isolated and tax- the complex linkages present in lignin to compounds of lower onomically identified using 16S ribosomal RNA (rRNA) se- molecular weight. (lu.se)
  • Marraffini, L. A. & Sontheimer, E. J. CRISPR interference: RNA-directed adaptive immunity in bacteria and archaea. (nature.com)
  • Mycoplasma species have also been mistakenly believed to be L-forms of bacteria, which also lack cell walls. (medscape.com)
  • The ProQ/FinO family of RNA binding proteins mediate sRNA-directed gene regulation throughout gram-negative bacteria. (nature.com)
  • Even the bacteria themselves are threatened by pathogens: Certain viruses, the bacteriophages (literally, "bacteria eaters"), have become specialized to invade bacterial cells and proliferate inside of them. (phys.org)
  • In order to get rid of these unwanted guests, many species of bacteria make use of an arsenal of molecules that works according to similar principles as an immune system does. (phys.org)
  • If care were not taken to inhibit metabolism of native bacteria colonizing the bladder, bacterial RNA of high specific activity predominated. (jci.org)
  • The bacteria is gram negative, rod shaped and aerobic belongs to the bacterial family of pseudomonadanceae. (ukessays.com)
  • Briefly, tagRNA-seq makes it possible to identify the 5' ends of RNAs in bacteria and directly probe for their type, primary or processed, by ligating short RNA sequences, the tags, to the beginnings of RNA molecules. (dissertations.se)
  • They trained the model on a dataset of around 50,000 guide RNAs targeting the genome of E. coli bacteria while also taking into account quantum chemical properties, in an approach described in the journal Nucleic Acids Research . (ornl.gov)
  • We determined whether Gram-negative bacterial molecules are associated with Alzheimer disease (AD) neuropathology given that previous studies demonstrate Gram-negative Escherichia coli bacteria can form extracellular amyloid and Gram-negative bacteria have been reported as the predominant bacteria found in normal human brains. (neurology.org)
  • The 6S RNA binds to RNA polymerase and regulates transcription, tmRNA has functions in protein synthesis, including the recycling of stalled ribosomes, 4.5S RNA regulates signal recognition particle (SRP), which is required for the secretion of proteins and RNase P is involved in maturing tRNAs. (wikipedia.org)
  • Here, we investigate the structural basis for RNA recognition by ProQ/FinO proteins, through the crystal structure of the ProQ/FinO domain of the Legionella pneumophila DNA uptake regulator, RocC, bound to the transcriptional terminator of its primary partner, the sRNA RocR. (nature.com)
  • More recently, the application of RNA-seq technologies has enabled the elucidation of the biological partners and targets of several ProQ/FinO family proteins. (nature.com)
  • Insight into how these proteins recognize their cognate RNAs initiated with FinO. (nature.com)
  • The study identified the ways in which proteins cross a cell membrane, a finding that could create a scientific foundation for better ways of delivering drugs into cells in the future, or for treating illnesses caused by bacterial toxins. (scitechdaily.com)
  • RNA codon misreading [formation of toxic, non functional proteins]. (mindmeister.com)
  • These proteins do not bind TMV RNA or form disks at pH 7. (whiterose.ac.uk)
  • Nitrofurantoin functions by inactivating bacterial ribosomal proteins causing inhibition of DNA, RNA, protein, and cell wall synthesis. (goldbio.com)
  • Penicillins inhibit bacterial cell wall synthesis by binding to penicillin-binding proteins. (medscape.com)
  • These types of RNA help assemble protein building blocks (amino acids) into functioning proteins. (medlineplus.gov)
  • Bacterial sRNAs affect how genes are expressed within bacterial cells via interaction with mRNA or protein, and thus can affect a variety of bacterial functions like metabolism, virulence, environmental stress response, and structure. (wikipedia.org)
  • It is now known that most bacterial sRNAs are encoded by free-standing genes located in the intergenic regions (IGR) between two known genes. (wikipedia.org)
  • Synthetic biology aims (among other goals) to harness these processes, to synthesize the switches and program the genes that would make a bacterial cell perform complex tasks. (innovationtoronto.com)
  • They created synthetic computation circuits by combining existing genetic "parts," or engineered genes, in novel ways, and implemented concepts from neuromorphic electronics into bacterial cells. (innovationtoronto.com)
  • Advances in transcriptome sequencing allow for simultaneous interrogation of differentially expressed genes from multiple species originating from a single RNA sample, termed dual or multi-species transcriptomics. (biomedcentral.com)
  • Now, Prof. Emmanuelle Charpentier's work group has scoured the genome of several hundred bacterial species in the search of CRISPR-Cas genes - and has made several discoveries. (phys.org)
  • We were able to identify new CRISPR-Cas genes in a number of bacterial species," says Charpentier, an HZI researcher who also teaches at Hannover Medical School (MHH). (phys.org)
  • TcdR, BotR, TetR and UviA are now known to be related alternative RNA polymerase sigma factors that drive transcription of toxin A and toxin B genes in C. difficile, the neurotoxin genes in C. botulinum and C. tetani, and a bacteriocin gene in C. perfringens. (pasteur.fr)
  • Here, we performed a comparative genomics study of RNA sensor genes in three species of pangolins. (frontiersin.org)
  • Gene Ontology analysis in both species, for the genes that exhibited inverse transcriptomic coordination during aging pointed to alterations in the perception of smell, a known impairment occurring during aging. (nsf.gov)
  • The most extensively characterized class of bacterial sRNAs are the trans -encoded sRNAs that are encoded distant from the genes for their mRNA targets and that typically have only limited complementarity (10-30 nt) [ 2 ]. (biomedcentral.com)
  • Other bacterial sRNAs, referred to as cis -encoded sRNAs, are present in close proximity to their targets, such as upstream, opposite of the 5′ UTR of the target, or between two genes in an operon [ 2 ]. (biomedcentral.com)
  • Following E. ictaluri challenge, the RNA-seq observed differentially expressed genes set indicated the centrality of actin cytoskeletal polymerization and junctional regulation in pathogen entry and subsequent inflammatory responses. (auburn.edu)
  • In a broader sense, viral and bacterial pathogens are among the strongest drivers of evolutionary change and the genomes of vertebrate species have been shaped, to a large extent, by adaptations to pathogens. (frontiersin.org)
  • Although several pathogens have been isolated and extensively studied, the complete infectome of the respiratory complex consists of a more extensive range unrecognised species. (bvsalud.org)
  • The freshwater bacterial pathogens Edwardsiella ictaluri, Aeromonas hydrophila and Flavobacterium columnare infect a variety of farmed fish species worldwide through various mucosal attachment points. (auburn.edu)
  • Emerging Bacterial and Mycotic Diseases Newly identified bacterial, mycobacterial, mycotic, and actinomycotic pathogens and known pathogens with unusual patterns of drug resistance constitute the focus of the Emerging Bacterial and Mycotic Diseases Branch. (cdc.gov)
  • Laboratory sections serve as international resources to identify and characterize bacterial and fungal pathogens. (cdc.gov)
  • Researchers found that the RNA sequences they analyzed contained a signature gene called RdRp that codes for RNA-dependent RNA polymerase-a complex enzyme designed to catalyze replication. (icr.org)
  • An early-morning sputum sam- encoding the B-subunit of RNA of droplet particles aerosolized from ple was collected from 170 patients polymerase ( rpoB DNA, 342-360 persons infected with Mycobacterium into wide-mouthed plastic contain- base pairs) was the target region for tuberculosis or by consumption of milk ers. (who.int)
  • RNA-sequencing, or RNA-seq, is used to analyze expression levels of all transcripts in a genome, including sRNAs. (wikipedia.org)
  • Analysis of average nucleotide identity and genome-to-genome distances, which have recently been proposed as reliable substitutes for classical DNA-DNA hybridization, further confirmed that these isolates should be classified as representatives of a novel species. (nih.gov)
  • Malone, C. D. & Hannon, G. J. Small RNAs as guardians of the genome. (nature.com)
  • The genetic material was inserted into the bacterial cell in the form of a plasmid: a relatively short DNA molecule that remains separate from the bacteria's "natural" genome. (innovationtoronto.com)
  • If this kind of enzyme is specifically equipped with new RNA "profiles," it cleaves the cell's genome at precisely defined sites. (phys.org)
  • The viral genome (total genetic information) can be RNA or DNA. (icr.org)
  • The CRISPR Cas9 tool relies on a single, unique guide RNA that directs the Cas9 enzyme to bind with and cleave the corresponding targeted site in the genome. (ornl.gov)
  • For contact tracing or inquiries related to antibiotic resistance, whole genome sequencing (WGS) of bacterial isolates is offered. (folkhalsomyndigheten.se)
  • Ribosomal RNA analyses demonstrated presence of additional bacterial and fungal species not identified with culture. (cdc.gov)
  • They used FISH probes informed by publicly available bacterial 16S ribosomal RNA databases to search for Fusobacterium species found in some vaginal samples in past studies. (genomeweb.com)
  • Heterodisperse RNA was obvious after 30 min of continuous labeling, but labeling of the 40S precursor of ribosomal RNA was not apparent for 60 min. (jci.org)
  • The recent developments in the taxonomy based on the 16s ribosomal RNA or conserved macromolecules, the family members are divided into eight groups. (ukessays.com)
  • This family is described by a Wikipedia entry Ribosomal RNA . (rfam.org)
  • ribosomal RNA identification of the isolates showed high score similarity with Pseudomonas spp. (lu.se)
  • The new kits complement the NEBNext products for RNA library preparation, including the Ultra II Directional RNA Library Prep Kit for Illumina and the NEBNext Library Quant Kit for Illumina, as well as the Monarch Total RNA Miniprep Kit, the company said. (genomeweb.com)
  • RNA-seq libraries were prepared from untreated and depleted RNA using the NEBNext Ultra™ II Directional RNA Library Prep Kit for Illumina®, followed by paired-end sequencing (2 x 75 bp). (neb.com)
  • sRNAs usually work by pairing with target mRNAs, often with the assistance of protein partners called RNA chaperones. (nature.com)
  • Abundant RNA species such as rRNAs and certain mRNAs can interfere with detection and analysis of biologically relevant RNAs of interest. (neb.com)
  • A limited study had indicated that in Sinorhizobium meliloti the YbeY ortholog regulates the accumulation of sRNAs as well as the target mRNAs, raising the possibility that YbeY may play a previously unrecognized role in bacterial sRNA regulation. (biomedcentral.com)
  • they are 50- to 500-nucleotide non-coding RNA molecules, highly structured and containing several stem-loops. (wikipedia.org)
  • It was also used to study polyadenylation in E. coli, where the ability to identify processed RNA molecules proved to be useful to separate direct and indirect regulatory effects of this mechanism. (dissertations.se)
  • They turned to quantum biology, a field bridging molecular biology and quantum chemistry that investigates the effects that electronic structure can have on the chemical properties and interactions of nucleotides, the molecules that form the building blocks of DNA and RNA. (ornl.gov)
  • The data show that Gram-negative bacterial molecules are associated with AD neuropathology. (neurology.org)
  • This damage results from a buildup of harmful molecules called reactive oxygen species, which are byproducts of energy production in mitochondria. (medlineplus.gov)
  • 2003. Evolutionary connection between the catalytic subunits of DNA-dependent RNA polmerases and eukaryotic RNA-dependent RNA polymerases. (icr.org)
  • Dual-species transcriptomics or dual-RNA-seq studies use transcriptomics to assess the transcriptional profiles of multiple organisms originating from the same sample [ 15 ]. (biomedcentral.com)
  • However, compared to typical transcriptomics studies, dual-species transcriptomics studies are technically challenging due to a difference in the proportion of reads from the major and minor organisms in the system, where major and minor refer to transcript abundance. (biomedcentral.com)
  • The organisms studied in dual-species transcriptomics experiments are present in different relative abundances and while the read proportions between the two organisms differ by system, most infection models, particularly biologically relevant models, have the total RNA content of the host vastly outnumbering microbe [ 15 ]. (biomedcentral.com)
  • This method is targeted to both gram-positive and gram-negative organisms, and is effective with RNA from monocultures or mixed bacterial species, such as those used in metatranscriptome studies, NEB said. (genomeweb.com)
  • Total RNA was extracted from a lyophilized pool of 20 different bacterial organisms (ATCC® #MSA-2002). (neb.com)
  • The normal human gut microbiota consists primarily of organisms from two bacterial phyla, Firmicutes and Bacteroidetes, representing 65% and 23% of organisms, respectively [ 3 ]. (springer.com)
  • Genetic and immunologic studies have helped branch researchers evaluate specific disease-causing factors in organisms such as those causing Brazilian purpuric fever and bacterial meningitis and have paved the way for the development of vaccines to prevent these and other diseases. (cdc.gov)
  • As with penicillins, inhibits third and final stage of bacterial cell wall synthesis by preferentially binding to specific PBPs located inside the bacterial cell wall. (medscape.com)
  • We intend to analyze each successive sequencing run to evaluate which host species are impacting sequencing results and an iterative approach to target additional sequences will be used. (usda.gov)
  • Conceptual parallels exist between bacterial and eukaryotic small-RNA (sRNA) pathways, yet relatively little is known about which protein may recognize and recruit bacterial sRNAs to interact with targets. (biomedcentral.com)
  • tracrRNA-mediated crRNA maturation is conserved among different bacterial species. (nature.com)
  • The team subsequently observed that two structurally different bacterial toxins also employed this same mechanism. (scitechdaily.com)
  • Structural analysis and RNA binding studies reveal that other ProQ/FinO domains also recognize related transcriptional terminators with different specificities for the length of the 3' ssRNA tail. (nature.com)
  • In order to characterize the immune actors and events taking place at the mucosal surfaces and identify the routes of pathogen attachment and entry, RNA-seq and microarray approaches were utilized to broadly investaged the transcriptional effects during bacterial infection in channel catfish. (auburn.edu)
  • We conclude that RNA metabolism in the toad bladder is extraordinarily slow, that a major acceleration of de novo synthesis in response to physiologic doses of aldosterone was not demonstrable, and that some reports to the contrary may have been influenced by artifacts from bacterial RNA metabolism. (jci.org)
  • Numerous sRNAs have been identified using both computational analysis and laboratory-based techniques such as Northern blotting, microarrays and RNA-Seq in a number of bacterial species including Escherichia coli, the model pathogen Salmonella, the nitrogen-fixing alphaproteobacterium Sinorhizobium meliloti, marine cyanobacteria, Francisella tularensis (the causative agent of tularaemia), Streptococcus pyogenes, the pathogen Staphylococcus aureus, and the plant pathogen Xanthomonas oryzae pathovar oryzae. (wikipedia.org)
  • Since these initial discoveries, over six thousand bacterial sRNAs have been identified, largely through RNA-sequencing experiments. (wikipedia.org)
  • Bacterial sRNAs have a wide variety of regulatory mechanisms. (wikipedia.org)
  • Small RNAs (sRNAs) control a variety of physiological responses across bacterial species 1 . (nature.com)
  • Small regulatory RNAs (sRNAs) play key roles in modulating gene expression in both prokaryotes and eukaryotes. (biomedcentral.com)
  • Like microRNAs (miRNAs) in eukaryotes, trans -acting bacterial sRNAs appear to recognize their targets by a seed-pairing mechanism using seeds as small as 6-7 nucleotides. (biomedcentral.com)
  • Here, we describe best practices for multi-species transcriptomics experiments from the initial experimental design to the downstream differential expression analysis, highlighting important considerations that should be taken for these multi-species analyses when compared to traditional single-species transcriptomics analyses. (biomedcentral.com)
  • Analyses of RNA-seq data obtained in the context of these experiments revealed subtle artifacts in the coverage signal towards gene ends, that we were able to explain and quantify based Kolmogorov's broken stick model. (dissertations.se)
  • Our previous RNA-seq library preparation required a minimum input of 500 ng total RNA, but the high efficiency of the NEBNext Ultra II kit has allowed us to reduce this to either 100 ng for a standard pipeline, or down to just 5 ng for a low input option, enabling RNA-seq to be carried out from just 5,000-10,000 cells. (neb.com)
  • Here, we used total RNA sequencing (i.e., metatranscriptomics) of nasal and nasopharyngeal swabs collected from animals with and without BRD from two cattle feedlots in Australia. (bvsalud.org)
  • This highly abundant RNA can conceal the biological significance of less abundant transcripts, and so its efficient and specific removal is desirable. (neb.com)
  • Overall, the most abundant RNA virus, was the bovine nidovirus. (bvsalud.org)
  • 2022. Cryptic and abundant marine viruses at the evolutionary origins of Earth's RNA virome. (icr.org)
  • These isolates are considered to represent a novel species, for which the name Leptospira mayottensis sp. (nih.gov)
  • Bacterial metabolism selected lignin model compounds (vanillin, vanillate, 4- hydroxybenzoate, p-coumarate, benzoate, and ferulate) was carried out with three of the Pseudomonas isolates and in addition with the strain Pseudomonas putida KT2440 from a Introduction culture collection. (lu.se)
  • ABSTRACT This study was done to characterize at the species level Mycobacterium spp. (who.int)
  • Each assay is based on an endpoint PCR amplification of a species-specific genetic region of the relevant microbe or a region of an individual microbial gene. (qiagen.com)
  • Following shifts in gut bacterial composition, the immune system surpasses microbial tolerance and progresses to an inflammatory state, which can induce tissue damage throughout the body and promote disease pathogenesis. (springer.com)
  • We wanted to improve our understanding of guide design rules for optimal cutting efficiency with a microbial species focus given knowledge of the incompatibility of models trained across [biological] kingdoms," Noshay said. (ornl.gov)
  • Eckert said her synthetic biology team plans to work with computational science colleagues at ORNL to take what they've learned with the new microbial CRISPR Cas9 model and improve it further using data from lab experiments or a variety of microbial species. (ornl.gov)
  • We sought to characterise the bacterial and fungal stool flora of preterm neonates and organism viability and evaluate any associations with NEC and sepsis. (bmj.com)
  • Total bacterial communities significantly differed between healthy infants and those with NEC or sepsis, with Sphingomonas spp. (bmj.com)
  • Exploration of viability and non-bacterial contributors to the total community may increase understanding of NEC and sepsis. (bmj.com)
  • Pneumococcal Disease As the most common bacterial cause of otitis media in children and a major cause of bacterial pneumonia, sepsis, and meningitis in adults, Streptococcus pneumoniae is responsible each year for at least one-third of the 24 million outpatient visits for otitis media and for 500,000 cases of pneumonia and meningitis in the United States. (cdc.gov)
  • Circular dichroism study of nine species of transfer ribonucleic acid. (colorado.edu)
  • 2 Viruses enter into a host cell for replication (the process of producing two identical replicas of RNA, ribonucleic acid or DNA, deoxyribonucleic acid) and take over the cell's biological machinery. (icr.org)
  • One such example is diphtheria toxin, which is produced by Corynebacterium diphtheriae and causes diphtheria, a serious and potentially fatal bacterial infection of the nose and throat. (scitechdaily.com)
  • Previous research has shown that infection with coronaviruses activates an innate immune response upon sensing of viral RNA by interferon-induced with helicase C domain 1 (IFIH1), also known as MDA5. (frontiersin.org)
  • The availability of transcriptomic tools including microarrays and RNA-Seq has recently opened a window through which we can observe the complexity of fish host-pathogen interactions during infection. (auburn.edu)
  • In vivo and in vitro with either uridine-5-3H or with methionine-(methyl)-3H as precursors, processing of RNA was extremely slow. (jci.org)
  • For use with NEBNext Multiplex Oligos for Illumina (Unique Dual Index UMI Adaptors RNA Set 1) ( NEB #E7416 ), refer to the Protocols tab for UMI Adaptors-specific guidance. (neb.com)
  • The highly conserved bacterial YbeY RNase has structural similarities to the MID domain of AGOs. (biomedcentral.com)
  • For single cell and ultra-low input RNA, our separate template-switching-based workflow generates full length cDNAs, followed by conversion to sequence-ready libraries using the Ultra ™ II FS workflow, that incorporates enzymatic DNA fragmentation. (neb.com)
  • The method is effective with intact and degraded RNA, from monocultures or samples with mixed bacterial species (e.g., metatranscriptomic). (neb.com)
  • Here, differential RNA sequencing of the human pathogen Streptococcus pyogenes uncovered tracrRNA, a trans -encoded small RNA with 24-nucleotide complementarity to the repeat regions of crRNA precursor transcripts. (nature.com)
  • Among these species are much-feared germs like Streptococcus pyogenes and the meningitis pathogen, Neisseria meningitidis . (phys.org)
  • Along with scientists from the Gwangju Institute of Science and Technology (GIST), the study takes a closer look at the Streptococcus ​ species and its role in skin health. (cosmeticsdesign-europe.com)
  • Conclusions Total and viable communities differ in preterm infants, and non-viable fungal species are present in infants on fungal prophylaxis. (bmj.com)
  • To investigate the clinical importance of a positive Pneumocystis -PCR among HIV-uninfected patients suspected of bacterial pneumonia, a retrospective matched case-control study was conducted. (biomedcentral.com)
  • Respiratory samples from 367 patients suspected of bacterial pneumonia were analysed by PCR amplification of Pneumocystis jiroveci . (biomedcentral.com)
  • The aim of the current study was to evaluate the clinical significance and prevalence of a PCR positive signal in a broad spectrum of clinical samples from HIV-negative patients with suspected bacterial pneumonia by means of an age and sex matched nested case-control study. (biomedcentral.com)
  • The remaining 106 respiratory samples represented all specimens submitted for investigation of bacterial pneumonia during a one-week period in August 1999, at the Department of Clinical Microbiology, Herlev University Hospital. (biomedcentral.com)
  • G- and Pseudomonas species some G+ not Sreptococcus pneumonia. (mindmeister.com)
  • In the 1960s, the abbreviation sRNA was used to refer to "soluble RNA," which is now known as transfer RNA or tRNA (for an example of the abbreviation used in this sense, see). (wikipedia.org)
  • Recognizing viral and bacterial diseases for which specific therapy is available is important. (medscape.com)
  • We also discovered evidences for circularization of a few RNA transcripts, both in our own data sets and publicly available data.Designing the tags used in tagRNA-seq led us to the problem of words absent from a text. (dissertations.se)
  • Compared to single-species differential expression analysis, the design of multi-species differential expression experiments must account for the relative abundances of each organism of interest within the sample, often requiring enrichment methods and yielding differences in total read counts across samples. (biomedcentral.com)
  • As library enrichment methods have improved, dual-species transcriptomics studies expanded to include the study of eukaryote-eukaryote and prokaryote-prokaryote systems. (biomedcentral.com)
  • Earlier evidence for obligatory alterations in RNA metabolism during the latent period is not strong. (jci.org)
  • We used the method to directly probe for transcription start and processing sites in two bacterial species, Escherichiacoli and Enterococcus faecalis. (dissertations.se)
  • The sam- target mycobacterial DNA or RNA directly ples were collected from patients with clin- from clinical samples [ 3 ]. (who.int)
  • Thus, the mechanism generating metabolic connectivity of the chromatophore fundamentally differs from the one for mitochondria and plastids, but likely rather resembles the poorly understood mechanism in various bacterial endosymbionts in plants and insects. (frontiersin.org)
  • Division of Bacterial and Mycotic Diseases --------------------------------------------------------------------------- The Division of Bacterial and Mycotic Diseases (DBMD), National Center for Infectious Diseases (NCID), Centers for Disease Control and Prevention (CDC), is dedicated to preventing and controlling the many emerging, re-emerging, drug-resistant, and other important bacterial and mycotic diseases in the United States and around the world. (cdc.gov)
  • Do you need increased sensitivity and specificity from your RNA-seq experiments? (neb.com)
  • Similarly, a minimal ProQ/FinO domain protein, NMB1681, has been shown to bind a range of structured RNAs in Neisseria meningitidis 15 . (nature.com)
  • DDX58/RIG-I, a sensor of cytoplasmic viral RNA and toll-like receptors (TLR) 3, 7, and 8, which bind RNA in endosomes, are conserved in pangolins. (frontiersin.org)
  • The way electrons are distributed in the molecule influences reactivity and conformational stability, including the likelihood that the Cas9 enzyme-guide RNA complex will effectively bind with the microbe's DNA, said Erica Prates, computational systems biologist at ORNL. (ornl.gov)
  • Bacterial abundance, community composition and production were also determined onboard. (awi.de)
  • SARS-CoV-2 detection status associates with bacterial community composition in patients and the hospital environment. (cdc.gov)
  • To cope with viral infections, vertebrate species have evolved response strategies which can be classified into resistance and tolerance ( 3 ). (frontiersin.org)
  • Species that tolerate infections can carry a high burden of infectious agents, and therefore may be important reservoirs for transmissions to other species. (frontiersin.org)
  • Candidemia accounted for 8% of all hospital-acquired bloodstream infections, and Candida species were the fourth most common cause of septicemia. (cdc.gov)
  • Chest radiography is used to evaluate the extent of pulmonary edema, to reveal secondary bacterial pulmonary infections, and to aid in ventilator management if intubation is required. (medscape.com)
  • Several RNA depletion products are available to selectively degrade and remove unwanted RNAs from your sample. (neb.com)
  • Our study reveals a novel pathway of small guide RNA maturation and the first example of a host factor (RNase III) required for bacterial RNA-mediated immunity against invaders. (nature.com)
  • Aliyari, R. & Ding, S. W. RNA-based viral immunity initiated by the Dicer family of host immune receptors. (nature.com)
  • Krzysztof Chylinski, Anaïs Le Rhun, Emmanuelle Charpentier, The tracrRNA and Cas9 families of type II CRISPR-Cas immunity systems, RNA Biology , 2013. (phys.org)
  • Chikungunya and dengue viruses cocirculate in Paraguay and surrounding countries, are transmitted by the same species of mosquitoes, and have similar clinical presentations during acute illness. (cdc.gov)
  • A group of strains representing species of the genus Leptospira, isolated from patients with leptospirosis in Mayotte (Indian Ocean), were previously found to be considerably divergent from other known species of the genus Leptospira. (nih.gov)
  • Two strains from each serogroup currently identified within this novel species were studied. (nih.gov)
  • Draft genomes of the strains were also analysed to study the DNA relatedness with other species of the genus Leptospira. (nih.gov)
  • mesenteroides strains at the species and subspecies levels. (univr.it)
  • mesenteroides strains at the species (rpoB-species-specific-PCR) and subspecies (phylogenetic comparison) levels. (univr.it)
  • A rapid protocol for the direct identification of Enterococcus faecalis and other Enterococcus species from a positive blood culture using a Peptide Nucleic Acid fluorescent in situ hybridization assay (PNA FISH). (jove.com)
  • Artificial Intelligence algorithms allowed the scientists to produce the required genetic modifications to the bacterial cells at a significantly reduced time and cost. (innovationtoronto.com)
  • This copy, a kind of " molecular profile " of bacteriophage DNA and other foreign genetic material , exists as RNA, an important cellular building block, which is used, among other things, as a temporary storage site of genetic information . (phys.org)
  • Prokaryotic evolution is influenced by the exchange of genetic information between species through a process referred to as recombination. (bvsalud.org)
  • To improve the modeling and design of guide RNA, the ORNL scientists sought a better understanding of what's going on at the most basic level in cell nuclei, where genetic material is stored. (ornl.gov)
  • Antibiotics are often used in clinical in vitro tests known as antimicrobial susceptibility tests or ASTs to determine their efficacy against certain bacterial species. (goldbio.com)
  • By contrast, IFIH1 a sensor of intracellular double-stranded RNA, has been inactivated by mutations in pangolins. (frontiersin.org)
  • Together, the enzyme and the profile RNA constitute the CRISPR-Cas system. (phys.org)
  • Characterisation of bacterial species from the transcriptome revealed a high abundance and diversity of Mollicutes in BRD cases and unaffected control animals. (bvsalud.org)
  • Of the non-Mollicutes species, Histophilus somni was detected, whereas there was a low abundance of Mannheimia haemolytica. (bvsalud.org)