Species polymorphism single stranded conformational. Medical search. Frequent questions

These species have been identified, either togeth- strand conformational polymorphism (SSCP) banding er or separately, in areas where RMSF is endemic. (cdc.gov)
In search of a quick and reliable HPG classification, three different molecular approaches were tested: the restriction fragment length polymorphism (RFLP) method applied to mtDNA control region (CR), the single strand conformational polymorphism (SSCP) method applied to NADH dehydrogenase subunit 2 (MT-ND2) and to NADH dehydrogenase subunit 4 (MT-ND4) regions. (metu.edu.tr)

Because alternative RNA splicing allows different types of mRNA molecules to be created from a single gene, it generates the diversity of protein function and structure that is essential to complex organisms. (genomicglossaries.com)
Our results show that one out of 60 bulls tested exhibited polymorphism (G→T) at position 559 in exon 4 of SLC35A3 gene. (researchgate.net)
In this paper, we have targeted a 27-bp AluI fragment of porcine mitochondrial cytochrome b (cytb) gene to construct porcine-specific hybrid nanobiosensor probe that was able to detect pork in heterogeneous autoclaved meat products in a single step eliminating the need of washing or separation steps. (hindawi.com)
High density arrays of extraordinarily sensitive integrated microring resonators will allow many gene and protein signatures to be simultaneously quantitated from a single patient sample. (nih.gov)
Fontsere C, de Manuel M, Marques-Bonet T, Kuhlwilm M. Admixture in mammals and how to understand its functional implications: on the abundance of gene flow in mammalian species, its impact on the genome, and roads into a functional understanding. (upf.edu)

Schistosoma haematobium and S. bovis are widespread schistosome species causing human and cattle schistosomiasis, respectively, in Africa. (imperial.ac.uk)
A single forward primer and two species-specific reverse primers were used to produce a polymerase chain reaction (PCR) fragment of 306 bp and 543 bp for S. bovis and S. haematobium, respectively. (imperial.ac.uk)
This RD-PCR proved highly sensitive, detecting a single larval stage and as little as 0.78 ng of genomic DNA (gDNA) from an adult schistosome, providing a cost-effective, rapid and robust molecular tool for high-throughput screening of S. haematobium and S. bovis populations. (imperial.ac.uk)

Quality of the modified ed, and researchers have suggested that "every rickettsial hexadecyltrimethylammonium bromide (CTAB) DNA species may have pathogenic potential, provided that its extractions was verified by amplifying a tick 16S mtDNA reservoir arthropod is capable of biting humans" (1,2). (cdc.gov)
In view to find putative mitochondrial genes in alternative reading frames on both coding and "presupposed" non-coding strands, numerous complete mtDNA genomes have been analyzed. (biomedcentral.com)

PCR single-stranded conformational polymorphism analysis demonstrated that these upstream flanking sequences and the 231-bp, 5' coding region are highly conserved among both drug-sensitive and multiply-drug-resistant isolates of M. tuberculosis. (bvsalud.org)
Hormones of the glucagon family have a common evolutionary origin that is reflected in high homology among their primary sequences in all vertebrate species 17 (Fig. 1b and Supplementary Fig. 1 ). (nature.com)
Nanoscaffolding and nanoquenching properties of thiol-capped gold nanocrystals (GNCs), covalently linked to fluorophore-lebeled oligonucleotide through metal-sulfur bond, were extensively studied for decades to detect specific sequences and single-nucleotide mismatches [ 1 - 3 , 21 , 22 ]. (hindawi.com)
Furthermore, the analysis of HPV6 E6 and E7 sequences revealed seven/five single-nucleotide mutations, two/four and five/one of which were non-synonymous and synonymous, respectively. (biomedcentral.com)
The formation of G-quadruplex DNA is easily conceivable in DNA sequences that are present as single strands in cells, such as the telomeric overhang. (hindawi.com)
Nevertheless, we have to keep in mind that these G-quadruplex-forming sequences although transiently single-stranded are a priori not as easily accessible as the telomeric sequences, due to the presence of the transcription machinery [ 28 ]. (hindawi.com)

It was found that single nucleotide mutation in the VRN-box can influence the vernalization requirement and growth habit of wheat. (biomedcentral.com)

After second- strand cDNA synthesis, T7 RNA polymerase was used to generate aRNA. (genomicglossaries.com)
The aim of this study was to detect carriers of CVM in Holstein population using Polymerase Chain Reaction- Primer-introduced restriction analysis and Restriction Fragment Length Polymorphism (PCR-PIRA and RFLP) methods. (researchgate.net)

We considered clusters of isolates with 5 single-nucleotide polymorphisms identified by whole-genome sequencing to indicate recent transmission and clusters of 10 persons to be outbreaks. (cdc.gov)

In spite of the biological and economic importance of plants, relatively few plant species have been sequenced. (mdpi.com)

citation needed] The studies that followed generally found that no specific "pathogenic" or nonpathogenic species of Blastocystis exists. (wikipedia.org)
However, efpA was not present in 17 other opportunistically pathogenic or nonpathogenic mycobacterial species. (bvsalud.org)

Single strand conformational polymorphism analysis, and the DNA sequence of the PCR product, shows differences between M. kansasii strains, supporting the suggestion that M. kansasii is not a homogeneous species. (aber.ac.uk)
The deduced protein product showed high sequence similarity to MalE-like proteins from a variety of bacterial species, including Mycobacterium leprae. (bvsalud.org)
The re-annotation also utilises sequence data from additional Campylobacter strains and species not available during the original annotation. (omictools.com)

G-rich DNA strands are naturally involved in duplex-DNA architecture through association with their complementary C-rich DNA strands by the canonical Watson-Crick pairing [ 1 - 3 ]. (hindawi.com)
A positionally conserved ORF has been found on the complementary strand of the cox1 genes of both eukaryotic mitochondria (protist, plant, fungal and animal) and alpha-proteobacteria. (biomedcentral.com)
This search has led to the observation of an unknown, positionally conserved open reading frame (ORF) on the complementary strand of eukaryotic cox1 genes. (biomedcentral.com)

Few plant species are dioecious and only a small fraction of these species are known to have sex chromosomes. (ox.ac.uk)
Each plant species has its own optimal temperature for growth, and its geographical distribution is determined to a major extent by the temperature zone in which it can survive. (allindianpatents.com)

We developed and used new single-molecule fluorescence spectroscopic and fluorescence lifetime imaging methods, combined with deep learning for image analysis. (biorxiv.org)
In this initial study, we combine single-molecule Förster resonance energy transfer (FRET) spectroscopy, fluorescence lifetime imaging (FLIM), and image analysis using deep learning (codes are available at https://github.com/hoisunglab/FNet ) to interrogate several steps during the aggregation process of the 42-residue Aβ peptide (Aβ42), including dimerization, formation of stable oligomers, and fibril elongation. (biorxiv.org)

We obtained six nanobodies with a broad specificity, that recognize strains belonging to the two most relevant species, Campylobacter jejuni and Campylobacter coli . (biomedcentral.com)

The sympatric occurrence of these two species and their ability to infect the same Bulinus intermediate snail hosts necessitates precise methods of identification of the larval stages. (imperial.ac.uk)
A related, often sympatric species is Borrelia bissettii , but this species has not been associated with human disease. (cdc.gov)

Such magnetic relaxation dispersion (MRD1) structural polymorphism of quadruplexes ( 2, 5, 6 ), but little measurements have been used to study ion binding (via 23Na is known about the energetics and kinetics of ion binding. (lu.se)

Mitochondria are both sources and targets of reactive oxygen species, and there is growing evidence that mitochondrial dysfunction may be a relevant intermediate mechanism by which cardiovascular risk factors lead to the formation of vascular lesions. (biomedcentral.com)
Mitochondrial DNA is probably the most sensitive cellular target of reactive oxygen species. (biomedcentral.com)
Similarly - in addition to being a cause of endothelial dysfunction, reactive oxygen species and subsequent mitochondrial dysfunction - hypertension may develop in the presence of mitochondrial DNA mutations. (biomedcentral.com)

This technique has the potential to identify toxic species. (biorxiv.org)

Indeed, we could not find any homologue to these genes in the genome of Hanseniaspora uvarum, H. guillermondii, Lachancea thermotolerans, Torulaspora delbreueckii, Metschnikowia pulcherrima, or Starmarella bacillaris species. (bvsalud.org)
The specialization of sterol import function for post genome-duplication species may have favored growth under anaerobiosis. (bvsalud.org)

Some researchers believe the debate has been resolved by finding of multiple species of Blastocystis that can infect humans, with some causing symptoms and others being harmless (see Genetics and Symptoms). (wikipedia.org)

The sterol uptake efficiency of 39 strains was evaluated by flow cytometry (with 25-NBD Cholesterol, a fluorescent cholesterol probe introduced in the medium) and we found an important discrepancy between Saccharomyces and non-Saccharomyces wine yeast species that we correlated to a lower final cell population and a lower fermentation rate. (bvsalud.org)

Polymorphism in the structure of fibrils formed by aggregation suggests the existence of many different assembly pathways and therefore a heterogeneous ensemble of soluble oligomers. (biorxiv.org)
Aggregation to form fibrils is also highly heterogeneous in terms of the number of strands in a fibril and the elongation speed and conformation of fibrils. (biorxiv.org)
This polymorphism indicates that the entire aggregation process, including oligomerization, should be heterogeneous, which complicates biophysical and biochemical characterizations of Aβ. (biorxiv.org)

Here we investigated sterol uptake efficiency and the expression of genes related to sterol import in Saccharomyces and non-Saccharomyces wine yeast species fermenting under anaerobic conditions. (bvsalud.org)
In some mtDNAs (e.g., nematodes and annelids), all genes are transcribed in the same direction, whereas, in others, both strands encode genes. (biomedcentral.com)

The human protein structure consists of a globular domain with three α-helices and a two-strand antiparallel β-sheet , an NH 2 -terminal tail, and a short COOH -terminal tail. (wikidoc.org)
The mechanism for conformational conversion to the scrapie isoform is speculated to be an elusive ligand -protein, but, so far, no such compound has been identified. (wikidoc.org)

Single-molecule spectroscopy can be an effective tool to probe heterogeneity because molecular species can be observed one at a time, so individual oligomers can be detected without separation. (biorxiv.org)

PrP Sc is a conformational isoform of PrP C , but this orientation tends to accumulate in compact, protease -resistant aggregates within neural tissue. (wikidoc.org)
these structures differ by strand orientation (grey dashed arrows) and loop arrangement (represented in orange, yellow, and red). (hindawi.com)

Serial dilutions were carried out on various lifecycle stages and species combinations to test the sensitivity and specificity of the primers. (imperial.ac.uk)

Blastocystis is a protozoal, single-celled parasite that inhabits the gastrointestinal tracts of humans and other animals. (wikipedia.org)
Prion diseases are rare, invariably lethal and rapidly progressive neurodegenerative disorders that affect humans and other species of mammals [ 1 ]. (biomedcentral.com)
It is the beginning of 21st century when the species of SARS-CoV start spreading the deadly pneumonia into in humans (Drosten et al. (pdfplayer.org)

Due to the nutritional content and commercial value of its seeds, Bertholletia excelsa is one of the most important species exploited in the Amazon region. (geneticsmr.com)

NMR shows copper binding results in a conformational change at the N-terminus . (wikidoc.org)

These data may then be analyzed using species-tree methods that use principles of population genetics to resolve incongruence among loci (e.g. (biomedcentral.com)
Length Polymorphism (PCR-PIRA and RF LP) methods. (researchgate.net)

Single strand conformational it an ideal PCR target to identify SFG Rickettsia infections. (cdc.gov)

This cross-sectional ever, the only species considered to cause human disease study examined the prevalence and composition of SFG in Maryland is Rickettsia rickettsii , the causative agent of Rickettsia in D. variabilis in Maryland. (cdc.gov)

To confirm this polymorphism, the PCR product was purified using ExoSAP-IT followed by sequencing by Applied Biosystems 3130XL Automated Sequencer using the ABI BigDye Ver 3.1. (researchgate.net)

The biosensor detected 0.5% and 1% pork in raw and 2.5-h autoclaved pork-beef binary admixtures in a single step without any separation or washing. (hindawi.com)

The greatest diversity of the new variants of VRN-B1 (designated as VRN-B1.f , VRN-B1.s and VRN - B1.m ) was found in the tetraploid and some hexaploid wheat species. (biomedcentral.com)

NMR spectroscopy or X-ray crystallography, but important width measurements at a single resonance frequency (or questions remain concerning the energetics, stability, and magnetic field strength), making it difficult to separate dynamics of this structurally diverse nucleic acid class. (lu.se)

In this paper, we have structurally and functionally integrated a 3-nm diameter citrate-tannate-coated gold nanocrystal to fabricate a novel class of species-specific nanobiosensor to determine pork adulteration in raw as well as processed mixed meats. (hindawi.com)

Anatomy2

Organisms4

Diseases4

Chemicals and Drugs80

Analytical, Diagnostic and Therapeutic Techniques and Equipment60

Psychiatry and Psychology1

Phenomena and Processes102

Disciplines and Occupations6

Anthropology, Education, Sociology and Social Phenomena1

Technology, Industry, Agriculture1

Information Science9

Named Groups3

Health Care13

Geographicals5

SSCP2

Gene5

Bovis3

MtDNA2

Sequences6

Mutation1

Polymerase2

Nucleotide1

Biological1

Pathogenic2

Sequence3

Complementary3

Plant species2

Analysis2

Campylobacter1

Sympatric2

Structural1

Mitochondrial3

Technique1

Genome2

Infect1

Strains1

Heterogeneous3

Genes2

Protein2

Molecular1

Orientation2

Sensitivity1

Humans3

Region1

Results1

Methods2

Target1

Study1

Product1

Separation1

Found1

Important1

Specific1