Somatic cell induced pluripotent stem cells. Medical search. Frequent questions

Reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) is an epigenetic phenomenon. (hud.ac.uk)
Gene set enrichment analysis (GSEA) was performed to identify pathways related to the differences and similarities between chemical therapies, aging signatures, and the OSK(M)-induced iPSCs using the Kyoto Encyclopedia of Genes and Genomes (KEGG), Reactome pathways, and HALLMARK genomic databases. (news-medical.net)
Researchers who looked at the effect of aging on induced pluripotent stem cells (iPSCs) found that genetic mutations increased with the age of the donor who provided the source cells, according to study results. (sciencedaily.com)
Researchers at the Scripps Translational Science Institute (STSI) and The Scripps Research Institute (TSRI) who looked at the effect of aging on induced pluripotent stem cells (iPSCs) found that genetic mutations increased with the age of the donor who provided the source cells, according to study results published by the journal Nature Biotechnology . (sciencedaily.com)
Our study highlights that increased risk of mutations in iPSCs made from older donors of source cells. (sciencedaily.com)
Researchers found that iPSCs made from donors in their late 80s had twice as many mutations among protein-encoding genes as stem cells made from donors in their early 20s. (sciencedaily.com)
Unexpectedly, iPSCs made from blood cells donated by people over 90 years old actually contained fewer mutations than what researchers had expected. (sciencedaily.com)
In fact, stem cells from those extremely elderly participants had mutation numbers more comparable to iPSCs made from donors one-half to two-thirds younger. (sciencedaily.com)
Using iPSCs for treatment has already been initiated in Japan in a woman with age-related macular degeneration," said paper co-author and STSI Director Eric Topol, M.D. "Accordingly, it's vital that we fully understand the effects of aging on these cells being cultivated to treat patients in the future. (sciencedaily.com)
Of the 336 different mutations that were identified in the iPSCs generated for the study, 24 were in genes that could impair cell function or trigger tumor growth if they malfunctioned. (sciencedaily.com)
For example, cells made from iPSCs for a bone marrow transplant would be potentially dangerous if they contained a TET2 gene mutation linked to blood cancer, which surfaced during the study. (sciencedaily.com)
When we proposed this study, we weren't sure whether it would even be possible to grow iPSCs from the blood of the participants in the Wellderly Study, since others have reported difficulty in making these stem cells from aged patients," Baldwin said. (sciencedaily.com)
Regenerated airway and alveolar cells derived from human iPSCs express proteins that match human respiratory cell biomarkers and play an important role in pathogenesis, such as ACE2 receptor protein in SARS-CoV-2 infections. (genengnews.com)
In 2012 Shinya Yamanaka and Sir John Gurdon were awarded the Nobel Prize for directly generating induced pluripotent stem cells (iPSCs) from somatic cells by introducing four genes, now collectively called the Yamanaka factors (Myc, Oct3/4, Sox2 and Klf4). (genengnews.com)
Regenerative medicine aims to derive specialized cell types from iPSCs that can provide disease models for therapeutic development and can be used to transplant, replace, engineer or regenerate diseased cells. (genengnews.com)
The variation among induced pluripotent stem cells (iPSCs) in their differentiation capacity to specific lineages is frequently attributed to somatic memory. (nih.gov)
These data demonstrated that variations in the hematopoietic differentiation capacity of iPSCs are not attributable to somatic memories of their origins. (nih.gov)
8 Specifically, Plaintiffs allege that Defendants, individually and acting in concert, make, 9 use, sell, offer for sale, and/or import induced pluripotent stem cells ("iPSCs") that infringe 10 one or more claims of the asserted patents.1 (Id. (justia.com)
212 ("iPSCs used by Defendants to make at least the iPSC-derived 13 natural kill (NK) cell platforms are made by a process that comprises at least each step of 14 claim 1 of the '856 Patent. (justia.com)
Plaintiffs allege that Fate is the 21 22 23 24 25 26 27 28 1 Induced pluripotent stem cells ("iPSCs") "are pluripotent stem cells generated from somatic cells by reprogramming. (justia.com)
No. 184, Counterclaims ¶ 43 ("iPSCs are generated in culture from somatic cells through the introduction of reprogramming factors that transform a somatic cell into a pluripotent state. (justia.com)
This microscopic image shows a cross section from a teratoma, generated in the lab by authors of a June 9 study in Stem Cell Reports that tested the quality of induced pluripotent stem cells (iPSCs). (neurosciencenews.com)
Teratomas, benign tumors containing the developing cells of different body parts, allowed researchers to see if the iPSCs could form the body's three basic germ cell lines - endoderm (gut region), ectoderm (epidermis, nerve tissue, etc.) and mesoderm (muscles, blood cells, etc. (neurosciencenews.com)
In a study published June 9 by the journal Stem Cell Reports , and funded by the National Heart, Lung, and Blood Institute, the multi-institutional research team reports on the comprehensive characterization of a large set of induced pluripotent stem cells (iPSCs). (neurosciencenews.com)
Specialized iPSCs are reprogrammed from adult skin or infant cord blood cells and can become any cell type in the body - a condition called pluripotency that mimics the function of human embryonic stem cells (hESCs). (neurosciencenews.com)
Although the technology to produce safe and effective iPSCs exists, study authors report they encountered an unexpected number of wobbly production processes for the cells. (neurosciencenews.com)
Researchers also compared the molecular and functional characteristics of iPSCs to human embryonic stem cells, which are used sparingly as a gold standard for benchmarking quality. (neurosciencenews.com)
Being pluripotent means iPSCs can generate cells from each of the three basic germ cell lines that form the body - endoderm (gut region), ectoderm (epidermis, nerve tissue, etc.) and mesoderm (muscles, blood cells, etc. (neurosciencenews.com)
NPCs can be differentiated from embryonic stem cells (ESCs) but also from induced pluripotent stem cells (iPSCs) (PMID: 11731782). (sanbio.nl)
These progenitors which are derived from either embryonic stem cells (ESCs) or healthy induced pluripotent stem cells (iPSCs) express wild-type levels of a-syn, thus making them equally susceptible to developing Lewy bodies over time. (lu.se)
The advent of iPSCs has opened up the possibility to graft patient-specific cells which most likely would circumvent the need for immunosuppression. (lu.se)

achieved the production of induced pluripotent stem cells by genetic reprogramming of human dermal fibroblasts (from a baby's foreskin). (wikipedia.org)
Primed hPSCs are treated with a mammalian target of rapamycin inhibitor (Torin1) for 3 h and dissociated to single cells, which are plated on mouse embryonic fibroblasts in 2iLI medium, a condition essentially the same for culturing mouse embryonic stem cells. (nature.com)
Passaging 40.0 times resulted in senescent fibroblasts with no growth over 14 days, morphological alterations typical of aged cells, and a rise in cell-cycle regulator p21 (CDKN1A) transcripts. (news-medical.net)
The researchers discovered that OSK expression in cells, such as murine and human fibroblasts, may significantly repair the epigenetic environment and patterns of gene expression of aged cells. (news-medical.net)
Mouse embryonic fibroblasts may be used to maintain and expand pluripotent stem cells in an undifferentiated state. (rndsystems.com)
Here, we report that a specific glycogen synthase kinase 3 (GSK-3) inhibitor, CHIR99021, can induce the reprogramming of mouse embryonic fibroblasts transduced by only two factors, Oct4 and Klf4. (ca.gov)
Applying MPTR to dermal fibroblasts from middle-aged donors, we found that cells temporarily lose and then reacquire their fibroblast identity, possibly as a result of epigenetic memory at enhancers and/or persistent expression of some fibroblast genes. (elifesciences.org)
Negligible immunogenicity of induced pluripotent stem cells derived from human skin fibroblasts. (1library.org)
By viral expression of neural fate determinants, it is possible to directly reprogram mouse and human fibroblasts into functional neurons, also known as induced neurons. (lu.se)
Secondly, we have developed an all-in-one vector-based strategy to convert adult human dermal fibroblasts directly from Huntington's disease (HD) patients and control individuals into induced neurons (iNs). (lu.se)

Martin GR. Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells. (springer.com)
Establishment in culture of pluripotential cells from mouse embryos. (springer.com)
Stem cells exist both in embryos and adult cells. (articlecity.com)
Embryonic stem cells, which must be extracted from embryos three to five days old (known as blastocysts, which contain only about 150 cells at this point of development). (articlecity.com)
Naive human pluripotent stem cells (hPSCs) can be used to generate mature human cells of all three germ layers in mouse-human chimeric embryos. (nature.com)
Established naive hPSCs are injected into mouse blastocysts, which produce E17.5 mouse embryos containing 0.1-4.0% human cells as quantified by next-generation sequencing of 18S ribosomal DNA amplicons. (nature.com)
The protocol is suitable for studying the development of hPSCs in mouse embryos and may facilitate the generation of human cells, tissues and organs in animals. (nature.com)
Transient inhibition of mTOR in human pluripotent stem cells enables robust formation of mouse-human chimeric embryos. (nature.com)
Gregory Kaebnick recently suggested in Bioethics Forum that apparent differences between induced pluripotent stem (iPS) cells and embryos created by somatic cell nuclear transfer may not be all that relevant. (thehastingscenter.org)
Efficient generation of embryonic stem cells from single blastomeres of cryopreserved mouse embryos in the presence of signalling modulators. (axonmedchem.com)
Embryonic stem (ES) cells are pluripotent stem cells derived from the inner cell mass of pre-implantation embryos. (rndsystems.com)
Depending on the source, stem cells can be classified into two broad categories i.e. embryonic stem cells that are derived from embryos and non-embryonic stem cells that are derived from adult and fetal tissues. (benthamscience.com)
The use of embryonic stem cells has been a source of considerable controversy due to its sacrifice of human embryos in the blastocyst stage, which some people view as the destruction of human life . (citizendium.org)
Human embryos fertilized in the ordinary manner and harvested in the blastocyst stage have been used as an extensive source of stem cells for research purposes, and have been shown to possess therapeutic value in laboratory animals. (citizendium.org)
The most infamous study of embryonic stem cells asserted that cloned human embryos had been created via somatic cell nuclear transfer, and stem cells had been generated from these embryos. (citizendium.org)
Ethical objections to the use of human embryonic stem cells revolve around the destruction of human embryos in the blastocyst stage to obtain the stem cells. (citizendium.org)

Yu is an important leader in developing novel method of reprogramming adult cells to create pluripotent cells which are not from embryonic stem cells. (wikipedia.org)
UW-Madison: University Communications: Introduction of Junying Yu and her work, including photos "UW-Madison News: November 2007, a new study led by UW scientist Junying Yu and conducted in Thomson's lab achieves genetic reprogramming of human skin cells to create cells indistinguishable from embryonic stem cells. (wikipedia.org)
In the field of induced potency and fate reprogramming, it remains unclear what the best starting cell might be and to what extent a cell need be transported back to a more primitive state for translational purposes. (nih.gov)
Reprogramming a committed cell back to pluripotence to then instruct it toward a particular specialized cell type is demanding and may increase risks of neoplasia and undesired cell types. (nih.gov)
In this Chapter, we dissect the proof-of-principle studies that demonstrated that cellular reprogramming to pluripotency can be induced in vivo, in spite of unfavorable pro-differentiation signals present within the tissues. (springer.com)
Thus, a much higher grade of parallelization, and throughput in the production of hiPSCs is needed, which can only be achieved by implementing automated solutions for cell reprogramming, and hiPSC expansion. (frontiersin.org)
We have developed a feeder-free, Sendai virus-mediated reprogramming protocol suitable for cell culture processing via a robotic liquid handling unit that delivers footprint-free hiPSCs within 3 weeks with state-of-the-art efficiencies. (frontiersin.org)
Induced pluripotent stem cells, which harness the power of genetic reprogramming - basically, the altering of a cell's DNA - to change the course of cellular development. (articlecity.com)
Successful reprogramming of epiblast stem cells by blocking nuclear localization of beta-catenin. (nature.com)
Chemically induced reprogramming to reverse cellular aging. (news-medical.net)
To gain new insights into pathogenesis of specific neurological and neurodevelopmental disorders, such as autism, pluripotent stem cells are derived by reprogramming of differentiated, mature cells of affected patients. (yale.edu)
Induced pluripotent stem (iPS) cells can be generated by somatic cell reprogramming following the exogenous expression of specific transcription factors (Oct-3/4, KLF4, SOX2, and c-Myc). (rndsystems.com)
Our portfolio includes human Mesenchymal stem cells, iPS cell lines, disease-specific iPS cell lines, growth and differentiation media, custom iPS reprogramming, and drug screening assay services. (cedarlanelabs.com)
Induced pluripotent stem cells, another types of pluripotent stem cells derived from any tissue by reprogramming and are the homologous source of stem cells. (benthamscience.com)
43.) "Four specific genes-cMYC, OCT3/4, SOX2 and KLF4-encoding transcription factors play a role in converting or reprogramming somatic cells into pluripotent stem cells. (justia.com)
2 The '369 Patent is entitled "Compositions for reprogramming somatic cells" and 3 was issued on December 6, 2011. (justia.com)
The '856 Patent is entitled 4 "Methods for reprogramming somatic cells" and was issued on January 13, 2015. (justia.com)
7 The '536 Patent is entitled "Methods for making somatic cells more susceptible to 8 reprogramming" and was issued on January 27, 2015. (justia.com)
Recent work has demonstrated that the epigenome is already rejuvenated by the maturation phase of somatic cell reprogramming, which suggests full reprogramming is not required to reverse ageing of somatic cells. (elifesciences.org)
This study describes a novel "maturation phase transient reprogramming" (MPTR) method to restore the epigenome of cells to a more youthful state. (elifesciences.org)
Studies that utilize genome-wide profiling methods which attempt to explain the differences between two or more experimental conditions such as cells treated with a drug vs. control, diseased tissue vs. normal, gene or protein expression at different time points during cellular differentiation or reprogramming, or candidate gene lists harboring mutations associated with a particular disease, produce lists of genes/proteins without apparent functional relationship. (biomedcentral.com)
To gain pluripotency, the reprogramming process typically involves transfecting adult somatic cells with certain pluripotency markers. (biomedcentral.com)
How well the 58 iPSC lines met quality criteria depended on the origin of the reprogrammed cells (skin vs. blood, male vs. female) and specific reprogramming methods. (neurosciencenews.com)
The different methods included a variety of reprogramming genes, vectors (engineered viruses that deliver genetic material to cells), or the use of plasmids (small DNA molecules that can deliver reprogramming genes). (neurosciencenews.com)
NPCs can additionally be obtained by direct reprogramming of somatic cells from different cell lineage (PMID: 22445518). (sanbio.nl)
Cellular reprogramming is a new and rapidly emerging field in which somatic cells can be turned into pluripotent stem cells or other somatic cell types simply by the expression of specific combinations of genes. (lu.se)
article{4b5db5d0-c1b6-463c-9ca2-f61b01429744, abstract = {{Cellular reprogramming is a new and rapidly emerging field in which somatic cells can be turned into pluripotent stem cells or other somatic cell types simply by the expression of specific combinations of genes. (lu.se)
Direct neuronal reprogramming of a somatic cell into therapeutic neurons, without a transient pluripotent state, provides new promise for the large number of individuals afflicted by neurodegenerative diseases or brain injury. (lu.se)
The first part of the thesis (Paper I, II, III) shows the development and improvement of a hESC-based system of for virus-mediated direct reprogramming of human glial progenitor cells into both induced dopaminergic neurons (iDANs) and GABAergic interneurons. (lu.se)
Reprogramming of somatic (e.g., skin or blood) cells is an emerging technology which gives the possibility to develop any cell type avoiding the ethical concerns with the use of human embryonic stem (ES) cells. (lu.se)
We are currently generating neurons by iPS and iN cell technology aiming to produce cortical neurons and after intracerebral transplantation or direct in vivo reprogramming of non-neuonal cells restore damaged neuronal network. (lu.se)
The model also predicts that reprogramming the network from a differentiated state, in particular the endoderm state, into a stem cell state, is best achieved by over-expressing Nanog, rather than by suppression of differentiation genes such as Gata-6. (lu.se)
It provides a framework to explore strategies of reprogramming a cell from a differentiated state to a stem cell state through directed perturbations. (lu.se)
Such an approach is highly relevant to regenerative medicine since it allows for a rapid search over the host of possibilities for reprogramming to a stem cell state. (lu.se)
Recent breakthroughs in reprogramming differentiated cells loops. (lu.se)
A computational model of master regulators that are required for successful reprogramming the dynamics of this core network has revealed that it functions as of a differentiated cell into a cell exhibiting stem cell like a bistable switch, which in the on state, corresponds to all these properties. (lu.se)

Here, we report the generation of NLRP3-mutant and non-mutant induced pluripotent stem cell (iPSC) lines from two CINCA syndrome patients with somatic mosaicism, and describe their differentiation into macrophages (iPS-MPs). (nih.gov)
The study assessed the genetic expression patterns of chemically treated cells compared to aged human-origin cells and OSK(MYC)-induced murine and human iPSC. (news-medical.net)
The Scientist brings together a panel of experts to educate you about the successes and caveats of using iPSC-derived cells in toxicological assays. (the-scientist.com)
The development of human induced pluripotent stem cell (iPSC)-derived cell lines as models for drug-toxicity testing offers a promising alternative that is more physiologically relevant, more predictive, and more time and cost efficient. (the-scientist.com)
In this study, we compared hematopoietic differentiation capacity of 35 human iPSC lines derived from four different tissues and four embryonic stem cell lines. (nih.gov)
162 ("Defendants' 11 use of their 'iPSC-derived cell therapy manufacturing platform' infringe[] at least claim 1 12 of the '369 Patent. (justia.com)
It gives scientists open access to data from the study to support their own research into potential iPSC-based stem cell therapies. (neurosciencenews.com)
It also is vital that - prior to being instructed to become a specific cell type - iPSC lines continuously renew and expand in a blank slate form without the introduction of genetic errors. (neurosciencenews.com)
Pluripotency can be tested to determine if iPSC lines are able to form what are called teratomas - benign tumors made up of different cell types (teeth, bone, brain, etc. (neurosciencenews.com)
It had been thought that poor quality iPSC lines could not be pluripotent, Lutzko said, although until the current study this theory had not been carefully tested because of the expense. (neurosciencenews.com)

Similarly, the transplantation of other tissue-specific stem cells, such as stem cells isolated from epithelial and neural tissues, can treat mouse disease models and human patients in which epithelial and neural cells are damaged. (articlecity.com)
Mouse nuclear transfer embryonic stem cells (NT-ESCs) were first established in 2000, and then proved to be able to differentiate either in vivo or in vitro, and give rise to individual tissues through germ line transmission or tetraploid complementation. (benthamscience.com)
endoderm, mesoderm and ectoderm, from which all somatic tissues in an organism can be traced. (edu.au)
Alternatively, NPCs can be isolated from fresh nervous tissues using cell sorting with NPC markers (PMID: 11121071). (sanbio.nl)

She previously worked as a postdoctoral scientist for two years in GlaxoSmithKline's Safety Pharmacology group, where her research focused on developing and validating an electrophysiology assay using human induced pluripotent stem cell-derived cardiomyocytes for cardiac safety screening. (the-scientist.com)
N-Glycans: Phenotypic Homology and Structural Differences between Myocardial Cells and Induced Pluripotent Stem Cell-Derived Cardiomyocytes. (stembook.org)
Functional and Electrical Integration of Induced Pluripotent Stem Cell-Derived Cardiomyocytes in a Myocardial Infarction Rat Heart. (stembook.org)
Transplantation of human induced pluripotent stem cell-derived cardiomyocytes is superior to somatic stem cell therapy for restoring cardiac function and oxygen consumption in a porcine model of myocardial infarction. (stembook.org)
Evaluation of the cardiotoxicity of mitragynine and its analogues using human induced pluripotent stem cell-derived cardiomyocytes. (1library.org)
Efficient and scalable purification of cardiomyocytes from human embryonic and induced pluripotent stem cells by VCAM1 surface expression. (1library.org)

Cell proliferation ceased, and differentiation into electrophysiologically active neurons and other CNS cell types in vivo ensued upon transplantation into rats, both during development and after adult injury--with functional improvement and without neoplasia, overgrowth, deformation, emergence of non-neural cell types, phenotypic or genomic instability, or need for immunosuppression. (nih.gov)
Transplantation of living nuclei from blastula cells into enucleated frogs' eggs. (springer.com)
Hepatocytes derived from iPS cells are suitable for transplantation into patients with liver insufficiency and for use in toxicity tests ( 2 ). (spandidos-publications.com)
For example, because hematopoietic stem cells can reconstitute the entire blood system, bone marrow transplantation has long been used in the clinic to treat various diseases. (articlecity.com)
Cross-species transplantation was possible without the rejection of the human embryonic stem cells by the mice's immune systems because the mice were genetically modified to suppress certain immune responses that would have interfered with transplantation. (citizendium.org)
Cell Spray Transplantation of Adipose-Derived Mesenchymal Stem Cell Recovers Ischemic Cardiomyopathy in a Porcine Model. (stembook.org)
Human induced pluripotent stem cells differentiation into oligodendrocyte progenitors and transplantation in a rat model of optic chiasm demyelination. (1library.org)
In addition, using iPS and iN cell technology patient-specific cells can be generated for transplantation, avoiding the need for immunosuppression and risk for rejection. (lu.se)
By linking together basic and clinical research, we aim to clarify cellular mechanisms of regeneration following damage to the brain and develop new therapeutic strategies to restore function in this organ (primarily in stroke and Parkinson's disease) by transplantation of stem cells or reprogrammed cells and optimization of endogenous repair mechanisms. (lu.se)
Our laboratory for the first time combines transplantation of stem cells, stimulation of endogenous neurogenesis and modulation of inflammatory responses in order to develop clinically effective cell replacement therapies for human neurodegenerative diseases. (lu.se)
Accumulated data indicate that hematopoietic stem cell transplantation may be effective under optimal conditions in preventing the progression of central nervous system symptoms in neuronopathic forms of lysosomal storage diseases, including some of the mucopolysaccharidoses, oligosaccharidoses, sphingolipidoses, and lipidoses. (medscape.com)
Although longitudinal natural history data are limited, published guidelines are available to assist with decisions related to the pursuit of transplantation and whether to use bone marrow or umbilical cord blood-derived cells. (medscape.com)
Some evidence indicates that at least in certain disorders, combination ERT and hematopoietic stem cell transplantation together might be superior to hematopoietic stem cell transplantation alone in patients who are appropriate candidates. (medscape.com)
The availability of both ERT and hematopoietic stem cell transplantation has prompted ongoing consideration of newborn screening efforts to diagnose lysosomal storage diseases. (medscape.com)

We have reprogrammed mesenchymal stromal cells from human bone marrow (iP-MSC) and compared their DNA methylation profiles with initial MSC and embryonic stem cells (ESCs) using high-density DNA methylation arrays covering more than 450,000 CpG sites. (hud.ac.uk)
Embryonic stem cells (ESCs) can grow infinitely and give rise to all types of cells in human body, thus of tremendous therapeutic potentials for a variety of diseases, such as Parkinson's disease, spinal cord injury, and diabetes. (benthamscience.com)
What is more, by deriving NT-ESCs from patient cells, the problem of immune rejection may be avoided. (benthamscience.com)

Precursor/progenitor cells from the organ of therapeutic concern typically lack only one critical attribute--the capacity for sustained self-renewal. (nih.gov)
we conditionally induced self-renewal efficiently within neural progenitors solely by introducing v-myc tightly regulated by a tetracycline (Tet)-on gene expression system. (nih.gov)
This strategy of inducing self-renewal might be applied to progenitors from other organs and may prove to be a safe, effective, efficient, and practical method for optimizing insights gained from the ability to reprogram cells. (nih.gov)
Regulatory microRNA sequences may stimulate self-renewal of these muscle cells. (natureasia.com)
They are able to differentiate into any cell of an organism and have the ability of self-renewal. (articlecity.com)
Stem cells are defined by their capacity for self-renewal and multilineage differentiation, making them uniquely situated to treat a broad spectrum of human diseases. (articlecity.com)
The ground state of embryonic stem cell self-renewal. (nature.com)
The related coactivator complexes SAGA and ATAC control embryonic stem cell self-renewal through acetyltransferase-independent mechanisms. (axonmedchem.com)
Pluripotent stem cells have the innate potential for unrestricted self-renewal and the ability to differentiate into any cell type in the body. (genengnews.com)
The term stem cell is also used in reference to any adult cells that are capable of assisting in the restoration of adult tissue via self-renewal. (citizendium.org)
Rb homeostasis is also essential for self-renewal and survival of human embryonic stem cells 10 . (biolegend.com)
Neural stem cells (NSCs) are undifferentiated neural cells with the capacity for long-term self-renewal and for differentiation into all types of neuronal and glial cells. (sanbio.nl)
Background: Recent studies have associated the transcription factors, Oct4, Sox2 and Nanog as parts of a self-regulating network which is responsible for maintaining embryonic stem cell properties: self renewal and pluripotency. (lu.se)

Viable offspring derived from fetal and adult mammalian cells. (springer.com)
Totipotent cells have the capacity to differentiate to all cell types, including somatic cells, germ cells, and certain cells that exist outside the embryo and are important to fetal development that are termed extraembryonic cells. (citizendium.org)
Inclusion of fetal bovine serum in stem cell media further contributes to an illdefined culture system. (ddw-online.com)
Transplantations of fetal tissue in the 1980s and 1990s provided proof-of-concept for the potential of cell replacement therapy for PD and some patients benefitted greatly from their transplants. (lu.se)

With the addition of four proteins, adult human skin cells can be transformed into neurons over a month-long period. (stanforddaily.com)
The researchers showed they could convert human embryonic stem cells to neurons by infecting them with a virus that expressed the same proteins used in the study. (stanforddaily.com)
This treatment, nicknamed "BAM" after an acronym of the three proteins, converted the embryonic stem cells into functional neurons within six days. (stanforddaily.com)
it triggered the skin cells' transformation into functional neurons within about four to five weeks. (stanforddaily.com)
The cells expressed electrical activity characteristic of neurons and even integrated and interacted with mouse neurons on a laboratory dish. (stanforddaily.com)
While they found that approximately 20 percent of mouse skin cells transform directly into functional neurons, under current culture conditions only about two to four percent of human skin cells do the same. (stanforddaily.com)
Furthermore, the use of diphtheria toxin -- which is far more toxic to human cells than mouse cells -- to destroy the human neurons in the mice reversed the observed improvements in motor function. (citizendium.org)
This result suggests that the observed increase in motor function was indeed produced by neurons derived from the human embryonic stem cells. (citizendium.org)
Figure 3) or PSA-NCAM are markers frequently used for intermediate progenitor cells and early immature neurons (PMID: 29625071). (sanbio.nl)
Generation of induced neurons via direct conversion in vivo. (lu.se)
The resulting cells are nonproliferating and present an alternative to induced pluripotent stem cells for obtaining patient- and disease-specific neurons to be used for disease modeling and for development of cell therapy. (lu.se)
Because of this local degeneration of a relatively small population of dopaminergic neurons in the midbrain, PD has been considered an especially interesting candidate for cell-replacement therapy. (lu.se)
First, we utilized single cell sequencing to dissect the differentiation of stem cells to midbrain dopaminergic neurons. (lu.se)
This approach could be potentially applied directly in the brain by targeting resident cells as a source of new neurons. (lu.se)
We are transplanting different types of neuroblasts generated either from human skin cell-derived induced pluripotent stem (iPS) cells or via direct conversion of skin cells to neurons (iN cells) (Fig.1). (lu.se)

Activation of muscle-specific genes in pigment, nerve, fat, liver, and fibroblast cell lines by forced expression of MyoD. (springer.com)
Found within Wharton's jelly - which is easily harvested from what would otherwise be post-natal medical waste - are several distinct stem cell genes. (articlecity.com)
We have learned that genes in mammalian cells are transcribed into messenger RNAs (mRNAs), which are to be translated into polypeptides (proteins). (intechopen.com)
For example, steroid and thyroid hormones directly bind to nuclear receptors, which induce expression of specific genes. (intechopen.com)
An aberrant miRNA expression could contribute to cancer development and progression [ 6 , 7 ] and could affect their target genes that are involved in many biological processes, such as cell differentiation, proliferation, apoptosis, metabolism, and development [ 8 ]. (hindawi.com)
The underphosphorylated, active form of Rb interacts directly with E2F1 , leading to cell cycle arrest, while the hyperphosphorylated form decouples from E2F1, thus promoting the transcription of genes promoting entry into the S phase. (biolegend.com)
Protein-protein, cell signaling, metabolic, and transcriptional interaction networks are useful for identifying connections between lists of experimentally identified genes/proteins. (biomedcentral.com)
This includes some cell lines contaminated with bacteria or carrying genes and mutations associated with cancer. (neurosciencenews.com)
The cells were generated with a variety of genes, methods and cells of origin, such as adult skin or infant cord blood cells. (neurosciencenews.com)
Role of indole curcumin in the epigenetic activation of apoptosis and cell cycle regulating genes. (cdc.gov)

Unmapped bibliographic data: ST - Induced pluripotent mesenchymal stromal cell clones retain donor-derived differences in DNA methylation profiles [Field not mapped to EPrints] C2 - PMC3538313 [Field not mapped to EPrints] AD - Institute for Neurophysiology, Medical Center, University of Cologne, Cologne, Germany. (hud.ac.uk)
The authors of the present study previously demonstrated that ectopically induced transcription factors, Octamer-binding transcription factor 4 (OCT-4), sex-determining region Y-box 2 (SOX2), and Kruppel-like factor 4 ( KLF-4) (collectively known as OSK factors) among mammals can reverse aging by restoring youthful patterns of deoxyribonucleic acid (DNA) methylation, transcriptomic profiles, and tissue functioning without cell identity loss. (news-medical.net)
DNA methylation dynamics in human induced pluripotent stem cells over time. (1library.org)
DNA methylation profile discriminates sporadic giant cell granulomas of the jaws and cherubism from their giant cell-rich histological mimics. (cdc.gov)
Complex haploinsufficiency in pluripotent cells yields somatic cells with DNA methylation abnormalities and pluripotency induction defects. (cdc.gov)

Embryonic stem cell lines derived from human blastocysts. (springer.com)
Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells' (2007), by Junying Yu et al. (asu.edu)
On 2 December 2007, Science published a report on creating human induced pluripotent stem (iPS) cells from human somatic cells: "Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells. (asu.edu)
The bank will hold 5,000 patient and disease-specific stem cell lines and other human cell lines derived from adult sources. (pharmaceutical-technology.com)

Scaffolds play an important role in tissue engineering as a substrate that can mimic the native extracellular matrix and the properties of scaffolds have been shown to affect the cell behavior such as the cell attachment, proliferation and differentiation. (wjgnet.com)
Gene expression must be appropriately maintained to regulate development, differentiation, and proliferation of cells. (intechopen.com)
These cell types are capable of unlimited, undifferentiated proliferation in vitro and still maintain the capacity to differentiate into a wide variety of somatic cells. (rndsystems.com)

Generation of reprogrammed somatic cells, known as induced pluripotent stem (iPS) cells, was initially performed by ectopic expression of four transcription factors, however, now this process is becoming more streamlined by improved methods. (edu.au)

To understand further the genetic factors influencing red blood cells, we carried out a genome-wide association study of haemoglobin concentration and related parameters in up to 135,367 individuals. (natureasia.com)
While human induced pluripotent stem cells (hiPSCs) provide novel prospects for disease-modeling, the high phenotypic variability seen across different lines demands usage of large hiPSC cohorts to decipher the impact of individual genetic variants. (frontiersin.org)
Exploring the genetic, molecular and biological features of neural differentiation process from embryonic and patient-specific stem cells may offer an unprecedented chance to understand normal and abnormal human brain development and diseases, with implications for drug development and potential cellular replacement therapy. (yale.edu)
But what is not getting such wide reporting is the use of pluripotent stem cells (as well as many other types of cells and genetic engineering techniques) for reproductive purposes . (lifeissues.net)
Genetic background drives transcriptional variation in human induced pluripotent stem cells. (1library.org)
2. Nuclear transfer is a technique used to duplicate genetic material by creating an embryo through the transfer and fusion of a diploid cell in an enucleated female oocyte.2 Cloning has a broader meaning than nuclear transfer as it also involves gene replication and natural or induced embryo splitting (see Annex 1). (who.int)

Progenitor cells are similar to embryonic stem cells in their capacity to differentiate into various cell types. (ddw-online.com)

Heart muscle cells die en masse after injury, yet the adult mammalian heart retains little capacity to regenerate them. (natureasia.com)
Interspecies chimerism with mammalian pluripotent stem cells. (nature.com)
Molecular mechanisms of the initiation of transcription from TATA box have been well known as the most essential nuclear events in mammalian cells. (intechopen.com)

Human liver organoids are formed from the assembly of hepatocyte-like cells differentiated from iPS cells, human umbilical vascular endothelial cells and mesenchymal stem cells ( 9 ). (spandidos-publications.com)
Efficient commitment to functional CD34+ progenitor cells from human bone marrow mesenchymal stem-cell-derived induced pluripotent stem cells. (1library.org)

Stem cells have attracted much interest in tissue engineering as a cell source due to their ability to proliferate in an undifferentiated state for prolonged time and capability of differentiating to different cell types after induction. (wjgnet.com)
With this raw material, biomedical firms can create stem cell lines that, among other things, aid recuperation via the regeneration of tissue that has been lost or damaged. (articlecity.com)
Current research is focused on growing a wide range of new tissue from stem cells, including muscle, blood, brain, and cartilage cells. (articlecity.com)
Adult stem cells, which are present in small amounts in adult tissue but less adaptable than embryonic stem cells, making their use in medical treatments more challenging. (articlecity.com)
They have many of the positive characteristics of embryonic stem cells while sourcing material is far less challenging, since postpartum tissue can be used. (articlecity.com)
CET specializes in stem cell research, tissue engineering and cancer biology. (cedarlanelabs.com)
The use of the pluripotent and/or self-renewing qualities of stem cells is believed to have therapeutic benefits for the regeneration of tissue in humans. (citizendium.org)
Such tissue renewal may be accomplished via the use of adult stem cells, or embryonic stem cells, which may be derived from a human embryo in the blastocyst stage. (citizendium.org)
The Institute has launched its Give Cures programme that has created a network of doctors in several private clinics and hospitals around the country to recruit patients to procure tissue to create the stem cell lines. (pharmaceutical-technology.com)
CET already manufactures a variety of human somatic stem cells, induced pluripotent stem (IPS) cells, cancer cells and specialised tissue culture media to grow and differentiate stem cells. (pharmaceutical-technology.com)
A subset of NSCs is present in the developed nervous system and acts as a reservoir of cells for cell replacement and nervous tissue regeneration (PMID: 20110496). (sanbio.nl)
However, post-mortem analysis of transplanted tissue revealed accumulation of pathological Lewy bodies in a small subset of transplanted cells over time, revealing a host-to-graft disease propagation. (lu.se)

In the present study, researchers devised high-throughput cellular assays that can differentiate between young, older, and aged cells of the body, including transcriptomic aging clocks and real-time quantitative nucleocytoplasmic compartmentalization (NCC) assays, to identify compounds that can reverse the aging process without genomic alterations. (news-medical.net)
More recently, advances in medical biology have shown that the genomic state of a somatic cell can be altered or 'reprogrammed' to become pluripotent. (edu.au)
Bisulfite converted genomic DNA lysates from induced pluripotent stem cell and embryonic stem cell lines were hybridized to Illumina HumanMethylation450 BeadChip. (nih.gov)

Stem cells and regenerative medicine - future perspectives. (springer.com)
Stem cells in regenerative medicine: introduction. (springer.com)
Cells from the blood samples are reprogrammed into clinical-grade human induced pluripotent stem cells, which can be distributed to recipients for regenerative medicine. (wjgnet.com)
Induced pluripotent stem cell technology has attracted enormous interest for potential application in regenerative medicine. (ca.gov)
Stem cells are emerging as an important source of material for diseases in regenerative medicine. (benthamscience.com)
The study of biology of stem cells is the hallmark of the recent emerging field of regenerative medicine and medical biotechnology. (benthamscience.com)
The remarkable potential of stem cells to generate several hundred differentiated cell types is driving their use for regenerative medicine and for supporting the traditional drug discovery and development process (Figure 1). (ddw-online.com)
The use of various types of stem cells for research purposes to make disease "models" in the lab for regenerative medicine and for "therapies" to cure sick patients for diseases is constantly in the news. (lifeissues.net)

Pluripotent cells may differentiate to cells of most types, and multipotent cells are capable only of differentiating to certain types within a group of cells that perform similar functions. (citizendium.org)
The main property that sets ESC apart from other cells is their pluripotent nature, meaning they can give rise/differentiate to cells of the three primary germ layers (mesoderm, endoderm and ectoderm) [ 13 ]. (biomedcentral.com)

We speculated that this could be induced in a regulatable manner such that cells proliferate only in vitro and differentiate in vivo without the need for promoting pluripotence or specifying lineage identity. (nih.gov)
Derivation of pluripotent stem cells with in vivo embryonic and extraembryonic potency. (nature.com)
In addition, because the cells do not pass a stem cell intermediate, direct neural conversion has the potential to be performed in vivo. (lu.se)

The developmental capacity of nuclei taken from differentiating endoderm cells of Xenopus laevis. (springer.com)
Here, we employed a human pancreatic differentiation platform complemented with an shRNA screen in human pluripotent stem cells (PSCs) to identify potential drivers of early endoderm and pancreatic development. (mdpi.com)
Citation: Chickarmane V, Peterson C (2008) A Computational Model for Understanding Stem Cell, Trophectoderm and Endoderm Lineage Determination. (lu.se)

Use of progenitor cell lines can eliminate the need to culture challenging human ES cells. (ddw-online.com)
Part of the Progenitor Cell Biology Consortium, the scientists are working to make sure this growing area of medical research is grounded in safe and sound science. (neurosciencenews.com)

At the core of the network reside Oct4, Sox2 and Nanog, into embryonic stem cells [1,2,3,4,5], have made major inroads which form a self-organized core of the TFs maintaining into stem cell biology. (lu.se)

Given that OKSM (Yamanaka) factors convert somatic cells into induced pluripotent stem (iPS) cells, alterations in transcriptional state could affect destiny of the cells. (intechopen.com)

Human pluripotent stem cells, with their ability to proliferate indefinitely and to differentiate into virtually all cell types of the human body, provide a novel resource to study human development and to implement relevant disease models. (mdpi.com)

The stem cell field witnessed a genuine breakthrough when a combination of solely four transcription factors ( Oct3 / 4 , Sox2 , Klf4 and c-Myc, OSKM ) proved enough to revert, in vitro, the differentiated status of a variety of cell types back to pluripotency, giving rise to so-called induced pluripotent stem (iPS) cells. (springer.com)
Generation of human-induced pluripotent stem cells in the absence of exogenous Sox2. (ca.gov)
To our knowledge, this is the first time that human iPS cells have been generated from somatic cells without exogenous Sox2 expression. (ca.gov)
and cell differentiation ( SOX2 and TGFB3 ) as well as immunohistochemical assay for VEGFA, TP53, Bcl2, TGFB1, and Ki67 protein expression have been performed in 85 FFPE RCC tumor specimens. (hindawi.com)

We show that human and chimpanzee cells differentiate in a similar man¬ner and that the difference in interspecies protein abundance is higher than transcript-level differences, suggesting that post-transcriptional mechanisms play a role in the difference between human and chim¬panzee brain development. (lu.se)

To clarify the metabolic differences between iPS cells in HDI and ReproFF (undifferentiated state), a metabolome analysis was performed. (spandidos-publications.com)
Persistent donor cell gene expression among human induced pluripotent stem cells contributes to differences with human embryonic stem cells. (1library.org)

The capability of these cells to differentiate depends on the stem cell type, the regulation of gene expression by various transcription factors and interaction with the stem cell niche 1,4 . (biolegend.com)
Top PDF Morphologic and gene expression criteria for identifying human induced pluripotent stem cells. (1library.org)

The use of mouse feeder layers and animal serum are particularly problematic in the culturing of stem cells for possible therapeutic applications. (ddw-online.com)
In particular, scientific developments in areas such as iPS cells open new possibilities of research and, at mid term, of therapeutic applications, but they also bring new ethical challenges and problems requiring further reflection and debate. (lifeissues.net)

Automatically expanded hiPSCs exhibit normal growth characteristics, and show sustained expression of the pluripotency associated stem cell marker TRA-1-60 over at least 5 weeks (10 passages). (frontiersin.org)
The advent of the human induced pluripotent stem cells (hiPSCs) technology offers unprecedented opportunities for disease modeling, personalized medicine, and the development of new therapeutic interventions. (frontiersin.org)

RUNX1-deficient mice fail to generate hematopoietic stem cells. (biolegend.com)

Firstly, we present a robust 2-week protocol for the differentiation of human pluripotent stem cells (PSCs) into forebrain neural progenitor cells. (lu.se)

He expressed hope that one day the cells could be used for human therapies. (stanforddaily.com)
This full potentiality makes them particularly suitable for developing cell replacement therapies or establishing cellular model systems. (edu.au)
CET and JP2MRI hope the biobank stem cell lines will be better models to predict the outcome of drug therapies in patients and advance research to bring new drugs to the market much more quickly and cheaply. (pharmaceutical-technology.com)
As the promise of using regenerative stem cell therapies draws closer, a consortium of biomedical scientists reports about 30 percent of induced pluripotent stem cells they analyzed from 10 research institutions were genetically unstable and not safe for clinical use. (neurosciencenews.com)
It was very surprising to us the high number of unstable cell lines identified in the study, which highlights the importance of setting safety standards for stem cell therapies," said Carolyn Lutzko, PhD, senior author and director of translational development in the Translational Core Laboratories at Cincinnati Children's Hospital Medical Center. (neurosciencenews.com)
A good number of the cell lines we studied met quality standards, although the unexpected number of lines that did not meet these standards could not be used for clinical therapies. (neurosciencenews.com)
The data presented in this thesis may serve as valuable resources to help optimize future cell replacement therapies for patients suffering from PD. (lu.se)

What are neural stem cells (NSCs), neural progenitors, and neural precursors (NPCs)? (sanbio.nl)
Neural progenitors are precursor cells able to further differentiate into various neuronal and glial cells. (sanbio.nl)
Today, clinical trials using stem cell-derived dopaminergic progenitors have commenced. (lu.se)
Next, in order to study the potential of autologous cell replacement therapy we transplanted progenitors derived from a PD patient into a pre-clinical rat model. (lu.se)

Transcription factors have an important role in the ability of a cell to self-renew and also differentiate into most cell types, also known as pluripotency 1 . (biolegend.com)

We manufacture specialized media and cell lines, and provide contractual research services with normal and patient-specific stem cells. (cedarlanelabs.com)
CET, a stem cell biotech company, and non-profit organisation JP2MRI hope that the stem cell biobank will help overcome obstacles facing personalised medicines by enabling testing on patient-specific stem cells instead of the currently used model that involves animal testing and expensive clinical trials. (pharmaceutical-technology.com)

After 3-4 d, bright, dome-shaped colonies with mouse embryonic stem cell morphology are passaged in 2iLI medium. (nature.com)

Derivation of novel human ground state naive pluripotent stem cells. (nature.com)
Derivation of naive human embryonic stem cells. (nature.com)

Gene therapy is experimental but in the future may help correct both somatic and neurologic abnormalities in a lysosomal storage disorder. (medscape.com)

The method could detect the consequences of a genetically induced epigenetic reversal of age using lentivirus transduction and gene ontology (GO) analysis. (news-medical.net)

Today, it has become a distinct source of stem cells and, therefore, a critical element in advancing medical treatments for everything from hair loss, the treatment of wounds, and surgical procedures. (articlecity.com)

F urther complicating matters, human ES cells are typically co-cultured with feeder layers of mouse fibroblast cells. (ddw-online.com)

For example, neural progenitor cells derived from a human ES cell line are easily propagated and require less handling than human ES cells. (ddw-online.com)

Stability of Imprinting and Differentiation Capacity in Naïve Human Cells Induced by Chemical Inhibition of CDK8 and CDK19. (axonmedchem.com)

Every cell type has its own unique needs when grown in vitro and stem cells are no exception. (ddw-online.com)
The need to control differentiation of embryonic stem cells in vitro presents another set of challenges. (ddw-online.com)

The pathogenesis of CINCA syndrome patients who carry NLRP3 mutations as somatic mosaicism has not been precisely described because of the difficulty in separating individual cells based on the presence or absence of the mutation. (nih.gov)
Researchers said the reason for this could be tied to the fact that blood stem cells remaining in elderly people have been protected from mutations over their lifetime by dividing less frequently. (sciencedaily.com)
How troublesome these mutations could be depends on how well the stem cells are screened to filter out the defects and how they are used therapeutically, Torkamani said. (sciencedaily.com)

Anatomy59

Organisms13

Diseases11

Chemicals and Drugs56

Analytical, Diagnostic and Therapeutic Techniques and Equipment58

Phenomena and Processes59

Disciplines and Occupations9

Technology, Industry, Agriculture3

Humanities1

Information Science2

Health Care1

IPSCs31

Fibroblasts10

Embryos16

Reprogramming36

IPSC10

Tissues4

Cardiomyocytes6

Transplantation14

ESCs3

Self-renewal13

Fetal4

Neurons15

Genes10

Methylation5

Lines Derived4

Proliferation3

Known as induced1

Genetic6

Capacity to differentiate1

Mammalian3

Mesenchymal2

Tissue12

Genomic3

Regenerative medicine8

Differentiate to cells2

Vivo3

Endoderm3

Progenitor cell2

Biology1

Yamanaka1

Ability to proliferate1

SOX24

Transcriptional1

Differences2

Gene Expression2

Therapeutic applications2

HiPSCs2

Hematopoietic stem1

PSCs1

Therapies7

Progenitors4

Transcription factors1

Patient-specific stem cells2

Mouse embryonic1

Derivation2

Abnormalities1

Genetically1

Source of stem cells1

Typically1

Neural progenitor1

Differentiation Capacity1

Vitro2

Mutations3