• Hybridization assays involve labelled nucleic acid probes to identify related DNA or RNA molecules (i.e. with significantly high degree of sequence similarity) within a complex mixture of unlabelled nucleic acid molecules. (wikipedia.org)
  • The goal of this RFA is to stimulate the development of a new generation of methods for natural products chemistry, and in doing so, to reinvigorate the investigation of nature as a prolific source of small-molecules with the potential to interact with all of the proteins that participate in cellular processes in health and disease. (nih.gov)
  • Introduction of small molecules to the developing Drosophila embryo offers great potential for characterizing biological activity of novel compounds, drugs, and toxins as well as for probing fundamental developmental pathways. (jove.com)
  • We use the unique characteristics of small molecules to probe and selectively modulate cell motility. (nature.com)
  • We will discuss the structure and function of both proteins and small molecules in biological systems. (columbia.edu)
  • Small organic molecules function in energy production and creating building blocks for the components of cells and can also be used to perturb the functions of proteins directly. (columbia.edu)
  • Total Antioxidant Capacity Assay Kit ab65329 can measure either the combination of both small molecule antioxidants and proteins, or small molecules alone in the presence of our proprietary Protein Mask. (abcam.com)
  • Stabilization of quadruplex architecture by small molecules is thus emerging as a potential anticancer approach since it is thought to interfere with oncogenic expression and telomeric maintenance in cancer cells [ 11 - 13 ]. (hindawi.com)
  • Interestingly, several classes of small molecules have been developed that efficiently target G-quadruplex DNA [ 29 - 32 ]. (hindawi.com)
  • When antibodies, small molecules, or nucleic acids bind to the surface they affect the current flow, which is recorded as a change in impedence in the chip that carries the sample. (genengnews.com)
  • According to the new study, led by Dr Mats Nilsson of Uppsala University in Sweden, existing assays are not sensitive enough: molecules in rare cells can easily escape detection, and it is not possible to tell which of the molecules detected originate from which cells. (nanowerk.com)
  • Padlock probes can amplify the minority signals making it possible to directly identify genetic variation at the mRNA (messenger ribonucleic acid) level (in molecules produced by active genes) in cells in microscopic preparations. (nanowerk.com)
  • The Chemical Proteomics unit (ChemProt) is a national unit expert on supporting drug discovery and development by proteome-wide deconvolution of targets and action mechanisms of small molecules. (scilifelab.se)
  • ChemProt makes use of proteome-wide orthogonal approaches to find out how small molecules work when incubated in cell cultures and in lysates. (scilifelab.se)
  • and discusses the future applications of G-quadruplex interacting small molecules, including the development as potential drug candidates. (novapublishers.com)
  • In conventional solid-phase assays, the solid phase mainly aids in separating molecules that bind to the solid phase from molecules that do not bind to the solid phase. (justia.com)
  • Signalling of hybridization methods can be performed using oligonucleotide probes modified in-synthesis with haptens and small molecule ligands which act homologous to the capture and detection antibodies. (wikipedia.org)
  • In the sandwich hybridization ELISA assay format, the antigen ligand and antibodies in ELISA are replaced with a nucleic acid analyte, complementary oligonucleotide capture and detection probes. (wikipedia.org)
  • Thus, optimal salt concentration in hybridization assays varies dependent upon the length and base composition of the analyte, capture and detection probes. (wikipedia.org)
  • Albeit generic, the ligation probe is similar to a detection probe in that it is labelled with, for example, digoxigenin for downstream signalling. (wikipedia.org)
  • The kinase phosphorylates the 5'-end of the analyte and the ligase will join the capture probe to the analyte to the detection probe. (wikipedia.org)
  • The capture and detection probes in the DLA can thus be termed ligation probes. (wikipedia.org)
  • SBS 2007 Poster #PST1C016) that Kinase-Glo is much less prone to interference from library compounds than PKLight reagent, making it a superior ATP-detection reagent for identifying small-molecule modulators of kinases by HTS. (wtnnews.com)
  • Most recently, GeneXpert MTB/RIF (Cepheid) advanced nucleic acid-based detection by integrating PCR and a highly sensitive molecular-beacon assay into a single, automated system. (cdc.gov)
  • 5.7 Leach L, Zhu Y, Chaturvedi S. Development and Validation of a Real-Time PCR Assay for Rapid Detection of Candida auris from Surveillance Samples. (cdc.gov)
  • Therefore, primer dimers are an issue in reactions using both probe-based and SYBR Green I dye-based detection. (sigmaaldrich.com)
  • With dye-based detection such as SYBR Green I, primer dimers also affect assay specificity because the nonspecific DNA-binding dye will bind to the primers and be detected along with the desired product. (sigmaaldrich.com)
  • Here, we developed a nanoPCR assay for the rapid detection of brain-eating amoebae using various nanoparticles. (springer.com)
  • Using nanoparticle-assisted PCR, the results revealed that graphene oxide, copper oxide and alumina nanoparticles significantly enhanced PCR efficiency in the detection of pathogenic free-living amoebae using genus-specific probes. (springer.com)
  • Virus particles monitored by fluorescence spectroscopy: a potential detection assay for macromolecular assembly. (cuny.edu)
  • One problem with conventional multiplex assays is that they typically cannot detect more than about five analytes simultaneously, because of difficulties with simultaneous detection and differentiation of more than about five analytes. (justia.com)
  • Assays based on high-affinity detection of secretory TMKmyc levels in culture using specific antibodies are considered. (biomedcentral.com)
  • Incorporating "chemiluminiscent or fluorescent" strategies may enable "photo-detection of TMKmyc changes" and hence automation of the entire assay. (biomedcentral.com)
  • The ethanolic extracts of the plant were examined for the presence of bioactive components and their total flavonoid content, with focusing on quercetin detection using thin layer bioautography (TLB) and brine shrimp lethality assay (BSLA) for cytotoxicity. (who.int)
  • The specificity of the hybridization-ligation assay for ligation at the 3'-end is particularly relevant because the predominant nucleases in blood are 3' to 5' exonucleases. (wikipedia.org)
  • The dual ligation hybridization assay (DLA) extends the specificity of the hybridization-ligation assay to a specific method for the parent compound. (wikipedia.org)
  • Despite hybridization-ligation assay's robustness, sensitivity and added specificity for the 3'-end of the oligonculeotide analyte, the hybridization-ligation assay is not specific for the 5' end of the analyte. (wikipedia.org)
  • The specificity of the assay is confirmed by a panel of clinically relevant non-MTB bacteria, and the clinical utility is demonstrated by the measurements in MTB-positive patient specimens. (cdc.gov)
  • However, small molecule redox imaging tools are underdeveloped: their protein specificity, spectral properties, and applicability remain poorly precedented. (uni-muenchen.de)
  • Assays requiring high specificity are particularly vulnerable when performed without optimization and adequate controls. (sigmaaldrich.com)
  • There are a number of factors that can be altered to obtain optimum assay performance and thereby lead to higher molecular sensitivity, specificity and precision. (sigmaaldrich.com)
  • Specificity takes into account the homology of various primers and probes for the many different HPV genomes, given that there may be numerous species that share some of these sequences. (genengnews.com)
  • Existing methods for measuring transcript levels in single cells include RT-qPCR (1), single molecule counting using digital PCR (2) or hybridization probes (3, 4), and next generation sequencing (5). (justia.com)
  • The assay was a viable alternative approach of qPCR for detecting, quantifying and counting copy number of shorter size DNA sequences to address a wide ranging biological problem in food industry, diagnostic laboratories and forensic medicine. (hindawi.com)
  • An assay that has been designed such that all desirable design criteria were met ( PCR/qPCR/dPCR Assay Design ) is likely to perform well under a wide range of conditions. (sigmaaldrich.com)
  • The primer design can be reviewed with respect to the assay design guidance provided in PCR/qPCR/dPCR Assay Design . (sigmaaldrich.com)
  • This review provides an insight into real-time quantitative PCR (qPCR) assays today. (ddw-online.com)
  • and progress in applying qPCR assays to novel assay application areas. (ddw-online.com)
  • To complement that review, HTStec recently undertook a qPCR end user survey to understand current practices and preferences in qPCR assays and instruments, and to identify future user requirements (2). (ddw-online.com)
  • In this article we highlight some of the main findings of the resulting HTStec qPCR Assay Trends market report. (ddw-online.com)
  • It also provides an insight into how qPCR assays are typically undertaken today in many labs and what influences the choice in purchasing qPCR mastermixes and thermal cycling instruments. (ddw-online.com)
  • The alternative is purchasing qPCR bulk reagents separately, where the end user would optimise and validate each assay in their own lab. (ddw-online.com)
  • Thus, through 96-well or 364-well plates, Luminex ELISA multiplex assays provide higher throughput and density. (magazinexu.com)
  • Disclosed are materials and methods for performing multiplex assays for nucleic acids, in which a transponder is associated with the bead(s) forming the solid phase used in the assay, nucleic acid probes are bound to the surface of the particles, and data concerning the assay is encoded on the transponder. (justia.com)
  • Such assays are known in the art as multiplex assays. (justia.com)
  • Multiplex assays are performed to determine simultaneously the presence or concentration of more than one molecule in the sample being analyzed, or alternatively, several characteristics of a single molecule, such as, the presence of several epitopes on a single protein molecule. (justia.com)
  • Khan NA, Jarroll EL, Paget TA (2001) Acanthamoeba can be differentiated by the polymerase chain reaction and simple plating assays. (springer.com)
  • A multiplex polymerase chain reaction (PCR) assay was also developed as an alternative for identification of isolates, and was shown to be a rapid, sensitive and specific diagnostic assay. (who.int)
  • If you do not have Bicoid DNA, it is acceptable to substitute another non-human non-fungal control DNA in this assay along with the appropriate primers and probes. (cdc.gov)
  • These select chemical probes also cover pathways that are of interest in phenotypic drug-discovery (PDD) campaigns as they include compounds selective for common off-targets. (discoverx.com)
  • A hybridization assay comprises any form of quantifiable hybridization i.e. the quantitative annealing of two complementary strands of nucleic acids, known as nucleic acid hybridization. (wikipedia.org)
  • In the context of biochemistry and drug development, a hybridization assay is a type of Ligand Binding Assay (LBA) used to quantify nucleic acids in biological matrices. (wikipedia.org)
  • Antisense therapy, siRNA, and other oligonucleotide and nucleic acid based biotherapeutics can be quantified with hybridization assays. (wikipedia.org)
  • It possesses a universal sample-processing cartridge for conducting multiplexed assays, either nucleic acid or immunoassay-based, and is field deployable. (genengnews.com)
  • This invention relates to materials and methods for detecting nucleic acids in samples and, more particularly, to solid phase assays wherein transponders are associated with the beads constituting the solid phase, nucleic acid probes are bound to the surface of the particles and data concerning the assay is encoded on the transponders. (justia.com)
  • Solid phase assays have been used to determine the presence of nucleic acids, including deoxyribonucleic acids (DNA), ribonucleic acids (RNA) and their modified forms. (justia.com)
  • In an electronically-indexed multiplex assay of this invention, two or more classes of transponders, each encoded with a different index number and constructed to bind a different nucleic acid sequence, are incubated with the sample in a single vessel. (justia.com)
  • More recently, newer molecular assays for TB have emerged based on serology (detecting the 38 kDa antigen, Early Secretory Antigen or ESAT-6, CFP10 and other secretory antigens), nucleic acid amplification (NAATS), phage amplification and line probe assays for MDR [ 10 ]. (biomedcentral.com)
  • Hybridization assays can be in solution or on a solid support such as 96-well plates or labelled beads. (wikipedia.org)
  • The competitive hybridization assay is similar to a traditional competitive immunoassay. (wikipedia.org)
  • Like other hybridization assays, it relies on complementarity, where the capture probe competes between the analyte and the tracer-a labelled oligonucleotide analog to the analyte. (wikipedia.org)
  • In the hybridization-ligation assay a template probe replaces the capture probe in the sandwich assay for immobilization to the solid support. (wikipedia.org)
  • As for the hybridization-ligation assay, the DLA is specific for the parent compound because the efficiency of ligation over a bulge loop is low, and thus the DLA detects the full-length analyte with both intact 5' and 3'-ends. (wikipedia.org)
  • The limitations with the hybridization-ligation assay also apply to the dual ligation assay, with the 5'-end in addition to the 3'-end requiring to have a free hydroxyl (or a phosphate group). (wikipedia.org)
  • We identify suitable probe compounds for use in studies on the functional effects of PHD inhibition in cells and in animals. (ox.ac.uk)
  • It is intended that this Program promote the development of automated screening projects that can be submitted to the Molecular Libraries Screening Centers Network (MLSCN), which employs a library of chemical compounds that are part of the Molecular Libraries Small Molecule Repository. (nih.gov)
  • Conclusions/Significance: 12 compounds were tested in vitro for direct enzyme inhibition and in a whole blood assay. (uni-frankfurt.de)
  • Different compounds or more than one biological system can be tested simultaneously. (scilifelab.se)
  • Proteome Integral Solubility Alteration (PISA) Assay, high throughput method based on protein solubility/stability upon thermal treatment, with no need of chemical engineering of compounds (now up to 16-plex, i.e. 16 biological samples in one multiplex analysis) (Gaetani M et al. (scilifelab.se)
  • Comprehensive molecular, cellular and systemic-level assays all support a high level of selectivity. (nature.com)
  • We do so by demonstrating selectivity across comprehensive molecular, cellular, and systemic assays. (nature.com)
  • However, the precise cellular roles of the central oxidoreductase systems, including TrxR and Trx, remain inaccessible due to the lack of probes to selectively measure turnover by either of these proteins. (uni-muenchen.de)
  • By comparing a novel 1,2 dithiolane-based probe to the state-of-the-art probes, including commercial TrxR sensors, by screening a conclusive assay panel of cellular TrxR modulations, I clarified that 1,2 dithiolanes are not selective substrates for TrxR in biological settings (Nat Commun 2022). (uni-muenchen.de)
  • Proteins are the primary class of biological macromolecules and serve to carry out most cellular functions. (columbia.edu)
  • Selective small molecule probes for the hypoxia inducible factor (HIF) prolyl hydroxylases. (ox.ac.uk)
  • This addition to lack of histologic lesions in small bowel biopsy chronic infection is characterized by histologic in- samples or lack of clinical involvement of the gastrointes- dication of gastrointestinal involvement, determined by tinal tract. (cdc.gov)
  • DNA was extracted from heart valves, 200 µL of carriage of the organism to a wide spectrum of clinical body fluid (blood in a tube containing EDTA, saliva, or pathologic conditions, including acute and chronic infec- cerebrospinal fluid), small bowel biopsy samples, and 1 tions ( 1 , 2 ). (cdc.gov)
  • However, despite growing biological and clinical significance, little is known about the structural arrangement of this receptor or any of its family members. (uci.edu)
  • These findings suggest that nanoPCR assay has tremendous potential in the clinical diagnosis of parasitic infections as well as for studying epidemiology and pathology and environmental monitoring of other microbes. (springer.com)
  • These probes are well known, and many are approved drugs, facilitating the connection of assay results to clinical effects. (discoverx.com)
  • Chapter Six focuses on the latest advances in the development of the telomerase assays and the authors further discuss the application potentiality of the assays for clinical samples. (novapublishers.com)
  • The chemoproteomic toolkit is anchored by liquid chromatography-tandem mass spectrometry (LC-MS/MS or LC-MS) based quantitative proteomics , which allows for the near complete identification and relative quantification of complex proteome s in biological samples. (explained.today)
  • This process improves access to microfluidics for controlling biological microenvironments, and further enabling visual and quantitative analysis of fungal cultures. (springer.com)
  • Phenotypic chemical biology is the use of target-selective chemical probes and drugs in cell-based or in vivo assays for establishing target function and disease relevance. (discoverx.com)
  • These 17 probes represent the best of the best, covering a broad range of pathway and target mechanisms with probes that are potent and selective for their targets or target class. (discoverx.com)
  • Management and prognosis of disease requires the accurate determination of specific biomarkers indicative of normal or disease-related biological processes or responses to therapy. (mdpi.com)
  • Topics covered will include high-throughput assay development, screening, chemical library creation, high-throughput chemistry, affinity purification of target proteins and target validation, protein microarrays, molecular evolution, protein engineering and synthetic biology. (columbia.edu)
  • Cytokines are small proteins and peptides that are produced by the immune cells. (magazinexu.com)
  • The Protein Mask prevents Cu2 + reduction by proteins, enabling the analysis of only the small molecule antioxidants. (abcam.com)
  • Alimova, A. Katz, P. Gottlieb, and R. R. Alfano, "Proteins and Dipicolinic Acid Released During Heat Shock Activation of Bacillus subtilis Spores Probed by Optical Spectoscopy," Applied Optics 45 (3): 445-450. (cuny.edu)
  • Ubiquitylation describes the covalent attachment of a small 76-amino acid protein, ubiquitin, to other proteins. (ddw-online.com)
  • We study the interaction between proteins in biological fluids as well as single proteins to nanomaterials of different kinds. (lu.se)
  • The last two decades have witnessed major breakthroughs in the identification of genes, gene products, metabolic pathways, and signaling pathways, as well as progress in miniaturization and robotics, enabling the development of high-throughput mechanism-based biological assays. (mdpi.com)
  • MicroRNAs have increasingly emerged in recent years as key regulators of biological processes and pathways. (pharmaceuticalintelligence.com)
  • Countless biological processes allow cells to develop, survive, and proliferate. (uni-muenchen.de)
  • The course is intended to provide a foundation needed for advanced chemical biology research, i.e. the creation and use of chemical probes of biological processes and macromolecular function. (columbia.edu)
  • The method allows us to study biological processes in individual cells, as opposed to the average states of large numbers of cells,' explained Dr Nilsson. (nanowerk.com)
  • Microfluidic systems are well-suited for studying mixed biological communities for improving industrial processes of fermentation, biofuel production, and pharmaceutical production. (springer.com)
  • New assays with single-molecule sensitivity, the authors say, are therefore essential. (nanowerk.com)
  • Microfluidic systems provide numerous advantages for single cell analysis: economies of scale, parallelization and automation, and increased sensitivity and precision that comes from small volume reactions. (justia.com)
  • Several targets of high profile drug s have been identified using chemoproteomics, and the continued improvement of mass spectrometer sensitivity and chemical probe technology indicates that chemoproteomics will play a large role in future drug discovery . (explained.today)
  • Whenever primer dimer products are produced and amplified, they divert reaction components away from synthesis of the desired product, thereby reducing assay efficiency and sensitivity. (sigmaaldrich.com)
  • SPME is simple, can work with small sample sizes, solvent-free extraction technique that provides high sensitivity and excellent reproducibility. (utm.my)
  • Gen-Probe RNA test, interferon-gamma assay, urine-based antigen test). (cdc.gov)
  • However, all assays have a set of optimal conditions and these are dependent on the instrument, selected reagents (buffer conditions), primer concentration and/or annealing temperature (T a ), magnesium concentration and even ramp rate. (sigmaaldrich.com)
  • Since it is usual to run the assay on a selected instrument and under standard buffer conditions, most optimization procedures are focussed on modification of primer binding kinetics using primer concentration or annealing temperature (T a )/melting temperature (T m ). (sigmaaldrich.com)
  • The amount of ammonia in collected blood, urine, saliva, or other biological fluid samples can be affected by several mechanisms that may lead to erroneous ammonia concentration determinations. (cdc.gov)
  • I showed that molecular probes using them as reduction sensors can be mostly processed by thioredoxins while being stable against reduction by GSH. (uni-muenchen.de)
  • Our facilities provide the opportunity to study protein structure, molecular probes and drug design, system biology and molecular interactions in cells and tissues. (lu.se)
  • With the help of various forms of mass spectrometry, synchrotron radiation, protein production & labelling, and bioinformatics, our facilities provide the opportunity to study protein structure and dynamics, molecular probes and drug design. (lu.se)
  • Building on previous methods developed by the same team, the researchers were able to convert messenger RNA (mRNA) into a type of DNA molecule that can be detected by 'padlock probes', fluorescent probes which lock onto the DNA after hybridisation, making it possible to study DNA repair at the molecular level in individual cells. (nanowerk.com)
  • However, such probes would allow measuring their effective reductive activity apart from expression levels in native systems, including in cells, animals, or patient samples. (uni-muenchen.de)
  • Researchers employ several techniques for evaluating cytokines in biological samples. (magazinexu.com)
  • Biological samples are always limited. (magazinexu.com)
  • Thus, a large amount of data can be obtained from a significantly small volume of study samples. (magazinexu.com)
  • The assay can multiplex upto 100 tests in a single run and run upto 1000 study samples every day. (magazinexu.com)
  • Luminex assays can effortlessly evaluate and measure critical biological elements in study samples. (magazinexu.com)
  • Luminex assays are indeed crucial in identifying and quantifying cytokines from study samples. (magazinexu.com)
  • They can analyze a wide range of biological samples such as saliva, sputum, urine, cell and tissue lysate, plasma, and serum, making them a crucial screening modality. (magazinexu.com)
  • Measurement of the combined non-enzymatic antioxidant capacity of biological fluids and other samples provides an indication of the overall capability to counteract reactive oxygen species (ROS), resist oxidative damage and combat oxidative stress-related diseases. (abcam.com)
  • No studies so far practically explored the sequence and mismatch detecting power of the fluorophore-labeled-oligo-nanoparticle conjugates in heterogeneous biological samples. (hindawi.com)
  • Claims relating to the performance of assays should be verified under the conditions of the study, including test samples, specific reagents and the instrument of choice. (sigmaaldrich.com)
  • Dr. de Koning's research group evaluated panels of samples and observed that the accuracy was quite high, 98%, when the samples were tested in a comparison with a reference assay. (genengnews.com)
  • Procedures for the determination of ammonia in biological samples are found in Table 7-1. (cdc.gov)
  • Consequently, small-molecule drug discovery usually involves an iterative process of molecular design, chemical synthesis, biological assay and data analysis feeding directly into the next cycle, but this process always needs a chemical starting point. (ddw-online.com)
  • Since DNA duplication during interphase precedes microbial division, "para-DNA synthesis assays" could be used to predict impending microbial growth. (biomedcentral.com)
  • In vitro phenotypic assays are used in drug discovery as well as for toxicology and alternative methods research, thus a standard set of probes would facilitate assay cross-comparison. (discoverx.com)
  • The aim of this study was to define algorithms for predicting positive TB cultures using antibody-based assays of TMKmyc levels in vitro . (biomedcentral.com)
  • The line probe assay, for instance, colorimetrically detects multi-drug resistant (MDR) strains from PCR-amplified products MTB DNA. (cdc.gov)
  • Thus, the Luminex assay expands the reach of customers in evaluating cytokines in complex biological matrices. (magazinexu.com)
  • This technique is used extensively in the assay of ethanol and other solvents in blood and biological fluids as no sample preparation required. (utm.my)
  • We did so by considering that small chemicals have potent biological properties, that single atom changes in their structure can affect those properties, that chemical structure can be modulated, and that as such they constitute highly refined biological probes. (nature.com)
  • Hence, Luminex ELISAs can multiplex upto 100 analytes in a single assay well. (magazinexu.com)
  • In other words, the number of different analytes that may be assayed in a single solid phase assay is limited by the solid phase. (justia.com)
  • In particular, the invention relates to microfluidic devices and their uses and methods for assaying cells. (justia.com)
  • Several studies implicated that longer DNA targets are broken down to small fragments during extensive food processing causing failure of PCR-based identification methods [ 23 , 26 , 28 , 31 ]. (hindawi.com)
  • ChemProt has recently developed high throughput (HT) chemical proteomics methods which outperform the previous methods for target deconvolution of at least tenfold and decrease by the same factor the time of analysis of the sample amount needed for each biological replicate. (scilifelab.se)
  • These methods may also incorporate simple calculated properties, for example the so-called 'Rule of 5' (5) to ensure libraries stay within sufficiently druglike areas of chemical space as well as existing structure activity relationship data describing the interaction of the chemical matter with the principle biological target, as well as related targets. (ddw-online.com)
  • These activities by the PI may include, but are not limited to, assay adaptation required for HTS implementation in a Center, the performance of secondary and/or counter-screening assays in support of hit follow-up, and the provision of HTS reagents requiring special expertise of the PI in production. (nih.gov)
  • The NIH Molecular Libraries and Imaging Roadmap Initiative ( http://nihroadmap.nih.gov/molecularlibraries/index.asp ) is a major NIH effort to broaden access to rapid assay technologies. (nih.gov)
  • Single cells represent a fundamental biological unit. (justia.com)
  • Researchers can customize study probes on assay panels and develop custom Luminex assays for individual research needs. (magazinexu.com)
  • The idea was to develop a small set of probes to help researchers in assay development using 2D, 3D and even organs-on-a-chip validate their assays for a common set of target and pathway mechanisms. (discoverx.com)
  • Characterizing phenotypic assays with this set of probes will help researchers more quickly deconvolute the mechanisms of any hits. (discoverx.com)
  • We have also proposed a change to the overall testing strategy to a tiered approach that considers animal welfare and alternatives to using vertebrate animals in vivo, including in silico modelling, read across from earthworm bioaccumulation tests, an in chemico digestibility assay to assess bioavailable fractions and ex-vivo measurements on gut sacs of fish. (plymouth.ac.uk)
  • Chemoproteomics entails a broad array of techniques used to identify and interrogate protein - small molecule interactions. (explained.today)
  • Chemoproteomics also attempts to address the inherent challenge of drug promiscuity in small molecule drug discovery by analyzing protein-small molecule interactions on a proteome -wide scale. (explained.today)
  • The overall goal of the Initiative is to facilitate the creation of a public database of biological information about small molecule chemical structures, which then seeds the further development of small molecule pharmacological tools for biological research. (nih.gov)
  • The Assay Development for HTS Program is one component of this Initiative, and funds the development and adaptation of biological assays for use in automated high throughput molecular screening (HTS). (nih.gov)
  • For 2008, the NIH Blueprint for Neuroscience Research ( http://neuroscienceblueprint.nih.gov/ ) is providing administrative supplements to Neuroscience-directed Assay Development for HTS projects. (nih.gov)
  • It is expected that the funds will enable the Investigator to participate in probe development activities that are related to their project. (nih.gov)
  • We hope that the development of a standard set of probes for phenotypic screens will inspire the community and create wide acceptance of criteria for phenotypic assays. (discoverx.com)
  • In the INH resistance scenario, it is shown that despite the presence of optimal doses of INH in LJ to stop M. tb proliferation, bacilli grow and the resulting phenotypic growth changes in colonies/units are predictable through the TMKmyc assay. (biomedcentral.com)
  • have developed a sensitive test, called EPOCH (express probe for on-site cannabis inhalation), that allows THC quantification in saliva on-site in minutes. (harvard.edu)
  • Ultra-Glo was derived by directed evolution and is the key component in Kinase-Glo Assay and a suite of other bioluminescent assays for high throughput screening and profiling of small molecule compound libraries. (wtnnews.com)
  • Combined with portable systems, the magnetic barcode assay holds promise to become a sensitive, high-throughput, and low-cost platform for point-of-care diagnostics. (cdc.gov)
  • The Luminex assay offers both high-throughput and high-density sample analysis. (magazinexu.com)
  • We have targeted expression of the probes to specific subcellular compartments in Arabidopsis and tobacco leaves and have used these plants to uncover new aspects of plant response to high light and pathogens. (plymouth.ac.uk)
  • Furthermore, these collaborative research efforts span the widest possible range in terms of company size, from small-size high-tech companies, over mid-size companies with a strong local base, to large, multinational enterprises. (lu.se)
  • Working in a rather small high-tech early stage company is of course (to use the buzz words you would expect) challenging, exciting and fun. (lu.se)
  • Maurits de Koning, Ph.D., a scientist at DDL Diagnostic Laboratory discussed his company's efforts at validating a human papilloma virus genotyping assay. (genengnews.com)
  • Molecular testing strategies in non-small cell lung cancer: optimizing the diagnostic journey. (cdc.gov)
  • In concept, Luminex assays are numerous ELISA assays on a bead. (magazinexu.com)
  • The DLA can also be used for the determination of individual metabolites in biological matrices. (wikipedia.org)
  • Besides, these results are as accurate and reliable as conventional ELISA assays. (magazinexu.com)
  • Compared to conventional immunoassays, Luminex assays provide a wide dynamic range. (magazinexu.com)
  • Similarly, when multiple targets are to be detected simultaneously in multiplex reactions, assay conditions must be optimized to detect all targets equally. (sigmaaldrich.com)
  • The research, conducted by the NIH Chemical Genomics Center (NCGC), describes Ultra-Glo recombinant luciferase as approximately 90% less susceptible to small molecule inhibition compared to another commercially available luciferase. (wtnnews.com)
  • Conclusions/Significance: This study demonstrates that receptor-based virtual screening with a permissive ('fuzzy') pharmacophore model can help identify small bioactive agents for combating bacterial infection. (uni-frankfurt.de)
  • Assay optimization and validation are essential, even when using assays that have been predesigned and commercially obtained. (sigmaaldrich.com)
  • Validation of primer design is particularly important when adopting primers from a previous publication or using a commercially supplied assay. (sigmaaldrich.com)
  • Luminex ELISA assays produce accurate results similar to traditional ELISA assays. (magazinexu.com)
  • Our results herein can be used to guide future design of MutY/MUTYH specific probes to monitor the activity, or lack thereof, of MutY/MUTYH variants. (ucdavis.edu)
  • Editors select a small number of articles recently published in the journal that they believe will be particularly interesting to readers, or important in the respective research area. (mdpi.com)
  • Validation provides the data required to justify the continued use of the assay in further research projects 1 . (sigmaaldrich.com)