• Use of whole genome sequencing to estimate the mutation rate of Mycobacterium tuberculosis during latent infection. (tamu.edu)
  • The results suggest that P2XM may play a significant role in the proliferation and/or differentiation of skeletal muscle cells and that its altered expression may be involved in the development of some sarcomas. (drugbank.com)
  • Of these, three miRNAs were differentially expressed miRNAs and 18 miRNAs involved in sexual differentiation as determined by functional analysis with GO annotation and KEGG pathway analysis. (peerj.com)
  • The comparison with the recently sequenced genome of the African congener Latimeria chalumnae and with the available genomic resources of other vertebrates revealed a good reconstruction of full length transcripts and a high coverage of the predicted full coelacanth transcriptome. (biomedcentral.com)
  • The genome sequences of Arachis duranensis and Arachis ipaensis, the diploid ancestors of cultivated peanut. (tamu.edu)
  • Through cloning of functional p53-binding sites (p53-tagged sites) from the human genome, we isolated a novel gene inducible by wild-type p53. (drugbank.com)
  • Nora Chapman et al from the Enterovirus Research Laboratory, Department of Pathology and Microbiology, University of Nebraska Medical Centre, have shown that human enteroviruses Coxsackie B can naturally delete sequence from the 5' end of the RNA genome and that this deletional mechanism results in long-term viral persistence, which has substantially altered the previously held view (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2440640/?tool=pubmed). (investinme.org)
  • The FACS and INTACT methods require plant transgenic lines respectively expressing fluorescent proteins or the biotinylated nuclear envelope protein in the cell type of interest. (nature.com)
  • Some DEGs, such as those encoding transcription factors, disease course-related proteins, peroxidase (POD), chitinase and phenylalanine ammonialyase (PAL), were highly expressed in the infection stage. (bvsalud.org)
  • Even though the locus can be indicated, transcriptional prevent sequences block manifestation of yellowish fluorescent proteins (YFP). (cancercurehere.com)
  • Western blot assessment confirmed that myc tagged human SOD1 proteins were induced by doxycycline in these cell lines. (hormonespathway.com)
  • The deep RNA sequencing performed with Illumina technologies generated 145,435,156 paired-end reads, accounting for ~14 GB of sequence data, which were de novo assembled using a Trinity/CLC combined strategy. (biomedcentral.com)
  • In the current study, we sequenced two small non-coding RNA libraries made from the gonads of adult male and female budgerigars using Illumina paired-end sequencing technology. (peerj.com)
  • Over the last few years next generation sequencing technologies (NGS) have revolutionized the fields of genomics and transcriptomics, providing the opportunity to analyze genomes and transcriptomes with high sequencing depth in a relatively short time in comparison with Sanger sequencing. (biomedcentral.com)
  • Sanger sequencing of a dissected desmoid tumor demonstrates base pair edits culminating in amino acid changes corresponding the human S45F mutation in CTNNB1 associated with sporadic desmoid tumorigenesis. (xenbase.org)
  • Northern blot analysis showed that it was expressed predominantly in skeletal muscle. (drugbank.com)
  • Therefore, ven-1 expression is required during the embryonic stages when nerves and muscles differentiate and attach to the body wall. (wormbook.org)
  • This gene encodes a myosin light chain kinase, a calcium / calmodulin dependent enzyme, that is exclusively expressed in adult skeletal muscle. (wikipedia.org)
  • The MYLK2 gene expresses skMLCK more prevalently in fast twitch muscle fibers as compared to slow twitch muscle fibers. (wikipedia.org)
  • Global gene-expression analysis is carried out using different technologies that are either array- or sequence-tag-based. (biomedcentral.com)
  • Array probes are produced by polymerase chain reaction (PCR) amplification of selected cDNAs or designed on the basis of cDNA or gene sequence information. (biomedcentral.com)
  • Because no unique gene nomenclature or sequence identifier is used for all platforms, a database linking the different identifiers belonging to one gene and its orthologs is required. (biomedcentral.com)
  • In addition, the database can be used to annotate array probes with reference sequences and updated gene descriptions from UniGene. (biomedcentral.com)
  • A comparative analysis of 3495 differentially expressed genes (DEGs) in distinct cell types demonstrated that fad2 inhibited the expression of the cytokinin synthesis gene LOG in vascular cells, thereby repressing leaf growth. (bvsalud.org)
  • Hence, we designated the gene P2XM (P2X specifically expressed in skeletal muscle). (drugbank.com)
  • Although we detected no genetic alteration in the coding sequences, one of four rhabdomyosarcoma cell lines examined had completely lost expression of this gene. (drugbank.com)
  • Primary annotations based on sequence similarity are linked to networks of systematic annotation in Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) and can be queried and computationally utilized in downstream analyses. (biomedcentral.com)
  • Desmoid tumors were dissected from 3-mo-old postmetamorphic animals, and both CRISPR/Cas9 target sites were subjected to targeted amplicon sequencing to determine gene editing outcomes. (xenbase.org)
  • From cDNA libraries, we isolated a 345 bp cDNA clone corresponding to the C53A.2 gene assigned on WormBase. (wormbook.org)
  • HGF c Met signaling can induce paxillin phosphorylation at its tyrosine residue, which in turn promotes tumor progression by enhancing tumor cell migration and spread.ten Activating c Met mutations are already proven to increase paxillin phosphorylation in SCLC.5 Furthermore, paxillin has become proven to be very expressed, and its gene from time to time amplified or mutated in NSCLC 11. (hormonespathway.com)
  • When expressed in Xenopus laevis oocytes, hOAT3 mediated the transport of estrone sulfate ( K m = 3.1 μM), p -aminohippurate ( K m = 87.2 μM), methotrexate ( K m = 10.9 μM), and cimetidine ( K m = 57.4 μM) in a sodium-independent manner. (aspetjournals.org)
  • Xenopus laevis oocytes expressing hOAT1 supported probenecid-sensitive uptake of [ 3 H] p -aminohippurate ( K m = 4 μM), which was trans -stimulated in oocytes preloaded with glutarate. (aspetjournals.org)
  • The other two elongation factors, SseEF1A3 and SseEF1A4 , represent novel genes that are mainly expressed in gills and skin. (biomedcentral.com)
  • Approximately 24,988 individual cells with 10,249 expressed genes were classified into five major cell types. (bvsalud.org)
  • The aim of this study was to analyze the changes in differentially expressed genes (DEGs) and differential metabolites during A. flavus infection between Zhonghua 6 and Yuanza 9102 by transcriptomic and metabolomic analysis. (bvsalud.org)
  • The three mammalian genes encoding members of the caveolin family are similar in sequence (Table 1 ). (biomedcentral.com)
  • Although the evolutionary history of the caveolin genes has not been clearly defined, there are clues within their sequences and genomic organization to suggest possible mechanisms for their origin. (biomedcentral.com)
  • In this study, we report a novel population of SSCs with osteoblast-chondrocyte transitional (OCT) identities in the bone marrow endosteal space, which highly expresses Fgfr3 and contributes to both normal and aberrant osteogenesis. (nature.com)
  • A paternally expressed QTL affecting skeletal and cardiac muscle mass in pigs maps to the IGF2 locus. (tamu.edu)
  • All of these isoforms are commonly expressed in the kidney, and their potential roles in renal handling of organic anions have been indicated. (aspetjournals.org)
  • 2022). Fibronectin isoforms in skeletal development and associated disorders. (mcgill.ca)
  • These and many other functional genomics studies rely on full genomic sequence to establish well-annotated databases. (biomedcentral.com)
  • A cDNA clone was isolated from a human renal library and designated human organic anion transporter 1 (hOAT1). (aspetjournals.org)
  • These include cDNA clone libraries, Affymetrix GeneChips, and oligonucleotide sets for human, mouse and rat (Table 1 ). (biomedcentral.com)
  • The past two decades has seen major advances towards our current understanding of skeletal myogenesis (reviewed in Perry and Rudnicki, 2000 ). (biologists.com)
  • Many ovarian TVMs are not expressed in normal tissues or other tumors. (nih.gov)
  • Second-generation shRNA libraries covering the mouse and human genomes. (tamu.edu)
  • Comparative anchor tagged sequences (CATS) for integrative mapping of mammalian genomes. (tamu.edu)
  • Northern blot analysis revealed that hOAT3 mRNA is expressed in the kidney, brain, and skeletal muscle. (aspetjournals.org)
  • We describe a bioinformatics pipeline known as FunnyBase that has been used to store, annotate, and analyze 40,363 expressed sequence tags (ESTs) from the heart and liver of the fish, Fundulus heteroclitus . (biomedcentral.com)
  • Isolation of the cDNA encoding rat skeletal muscle myosin light chain kinase. (wikipedia.org)
  • No YFP-labeled cells had been recognized in the liver organ of mice treated using the control vector AAV8-CMV-RFP (B). AAV8-CMV-Cre tagged CK19-expressing cholangiocytes and HNF4-expressing hepatocytes (C,D). Yellowish arrowheads: YFP+CK19+ cholangiocytes. (cancercurehere.com)
  • Residues that match the sequence are highlighted in yellowish. (healthweblognews.info)
  • The bone marrow contains various populations of skeletal stem cells (SSCs) in the stromal compartment, which are important regulators of bone formation. (nature.com)
  • In this study, we isolated fad2 mutant leaf protoplast cells to perform single-cell RNA sequencing. (bvsalud.org)
  • Removal of floxed prevent sequences by Cre IKK-gamma (phospho-Ser376) antibody recombinase qualified prospects to long term labeling of transduced cells with YFP. (cancercurehere.com)
  • Open up in another window Shape 2 Quantification from the percentage of AAV8-CMV-Cre/YFP-labeled cells within CK19-expressing cholangiocytes and HNF4-expressing hepatocytes. (cancercurehere.com)
  • In the comma stage, it was expressed in hypodermal cells: Abpl/raapppp (H7), P1/2 L/R, P3/4 L/R, P5/6 L/R, P7/8 L/R, P9/10 L/R, and P11/12. (wormbook.org)
  • sequence rescued the Ven phenotype, and the HA epitope was detected in hypodermal cells (Figure 2B). (wormbook.org)
  • The signal consisted of short oblique parallel lines arranged in four rows along the lateral body wall, corresponding to the position of muscle cells. (wormbook.org)
  • Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells. (yeasen.com)
  • The highest levels of caveolin-1 (also called caveolin, Cav-1 and VIP2I) are found in terminally-differentiated cell types, such as adipocytes, endothelia, smooth muscle cells, and type I pneumocytes. (biomedcentral.com)
  • Caveolin-3 (Cav-3) has greater protein-sequence similarity to Cav-1 than to Cav-2, but it is expressed mainly in muscle cells, including smooth, skeletal, and cardiac myocytes. (biomedcentral.com)
  • Western blot assessment revealed that the expression of c Abl was better in cells expressing mutant SOD1s than cells expressing wild variety SOD1. (hormonespathway.com)
  • Spudich, the Douglass M. and Nola Leishman Professor of Cardiovascular Disease at the Stanford University School of Medicine, will receive the 2012 Albert Lasker Basic Medical Research Award for his trailblazing investigations of the molecular motors that drive our skeletal muscle contractions and heartbeats, enable our cells to divide, and power patrolling immune cells through our tissues. (stanford.edu)
  • check the tag ADOLESCENCE HN - 2008 BX - Nutrition in Adolescence FX - Adolescent Nutrition Physiology MH - Peritoneal Stomata UI - D054048 MN - A01.047.025.600.700 MN - A10.810 MS - Natural openings in the subdiaphragmatic lymphatic plexus in the PERITONEUM, delimited by adjacent mesothelial cells. (bvsalud.org)
  • Pannexin 1 (PANX1) is expressed in many tissue types including tissues of neural origin. (jcancer.org)
  • Panx1 in rodents), which forms homo-heptameric single membrane channels permeable to ion and metabolite [ 7 , 8 ], is expressed in many tissue types including tissues of neural origin [ 9 ]. (jcancer.org)
  • The forced expression of the transcript in body wall muscle tissue rescued the Ven phenotype as efficiently as the genomic fragment did (Figure 2C). (wormbook.org)
  • A phylogenetic tree of all known caveolins shows that the C. elegans Cav-1 sequence is only distantly related to all the others (Figure 1 ). (biomedcentral.com)
  • Main characteristics and sequence identities with other fish and mammalian eEF1As are described. (biomedcentral.com)
  • The ven-1 transcript encoded a polypeptide of 86 amino acid residues, including the signal sequence at the N-terminus, suggesting that VEN-1 is a secreted protein (Figure 1B). (wormbook.org)
  • We believe that is also essential to highlight the need for this finding for the potential treatment of skeletal muscles degenerative disorders, among which Duchenne muscular dystrophy (DMD) may be the emblematic case. (biomasswars.com)
  • In conclusion, this work is the first report of sex-biased miRNAs expression in the budgerigar, and provides additional sequences to the avian miRNAome database which will foster further functional genomic research. (peerj.com)
  • To investigate the functional significance of this interaction, we expressed green fluorescent protein fusion constructs encoding defined fragments of titin's M-line region and MURF-1 in cardiac myocytes. (rupress.org)
  • These changes include elongation, polarization, aggregation and fusion, which ultimately give rise to functional muscle. (biologists.com)
  • We also examined the temporal requirement of ven-1 activity by expressing the transcript using a heat shock promoter. (wormbook.org)
  • Two of these differ in the presence of juxtamembrane extracellular sequences which regulate the ability of TACE (TNF-alpha converting enzyme) to proteolytically cleave ErbB4 from the cell surface. (bio-techne.com)
  • Advances in single-cell RNA-sequencing technology have resulted in a wealth of studies aiming to identify transcriptomic cell types in various biological. (rna-seqblog.com)
  • For immunoprecipitation experiments, HA-tag or Myc-tag antibody-conjugated agarose beads had been purchased from Cellular Signaling Technology. (healthweblognews.info)
  • Here we show that, concurrent with a decrease in chondrogenesis, inhibition of p38 in limb bud cultures dramatically promotes muscle development. (biologists.com)
  • However, reverse transcription-polymerase chain reaction revealed hOAT1 expression in human brain and skeletal muscle, as well. (aspetjournals.org)
  • An integrated framework for cDNA library production, sequencing, quality control, expression data generation, and systems-level analysis is presented and utilized. (biomedcentral.com)
  • Here, we show that while PANX1 is expressed in NB of all stages, high PANX1 expression in high-risk NB is associated with a reduced survival probability. (jcancer.org)
  • They are expressed ubiquitously in mammals, but their expression levels vary considerably between tissues. (biomedcentral.com)
  • 2022). Proteolysis of fibrillin-2 microfibrils is essential for normal skeletal development. (mcgill.ca)
  • PAX5, a transcription element necessary for B cell development, was strongly expressed in most SCLC situations and appeared to upregulate c Met transcription. (hormonespathway.com)
  • 1,100 muscle-targeted RNAi interventions to comprehensively assess the function of 447 transcription factors in the developmental growth of body wall skeletal muscles in Drosophila. (sdbonline.org)
  • Researcher from University of Nice-Sophia Antipolis, Nice, France about iDeal ChIP-seq kit for Transcription Factors , iDeal ChIP-seq kit for Transcription Factors , iDeal ChIP-seq Kit for Transcription Factors , 24 SI for ChIPmentation , TAG Kit for ChIPmentation . (diagenode.com)
  • To solve this issue, we used the Diagenode ChIPmentation solution composed of iDeal ChIP-seq Kit for Transcription Factor, TAG Kit for ChIPmentation, and 24 SI for ChIPmentation. (diagenode.com)
  • A cDNA encoding a multispecific organic anion transporter 3 (hOAT3) was isolated from a human kidney cDNA library. (aspetjournals.org)
  • To compare experiments that are performed on these different platforms, array probes and sequence tags need to be linked. (biomedcentral.com)
  • RNA interference experiments using this cDNA sequence reproduced the Ven phenotype. (wormbook.org)
  • ErbB4 is expressed in normal skeletal muscle, heart, pituitary, brain and several breast carcinomas. (bio-techne.com)
  • It is unknown if these are 'normal' or 'tumor' vessels, expressing tumor vascular markers (TVMs). (nih.gov)
  • SAGE results in quantitative information connected to 10-14 base-pair (bp) sequence tags derived from the 3' ends of transcripts. (biomedcentral.com)