Size genomic library. Medical search. Frequent questions

The quality and purity of genomic DNA are analyzed by spectrophotometer and electrophoresis. (3hbiomedical.com)

A genomic library is a collection of overlapping DNA fragments that together make up the total genomic DNA of a single organism. (wikipedia.org)
Forty-three sequences containing simple sequence repeats or microsatellites were generated from an M13 library of total genomic mouse DNA. (ox.ac.uk)

Illumina genomic adapters were ligated and the sample was size-fractionated (250-350 bp) on a 2% agarose gel. (nih.gov)
Libraries were pooled and sequenced on an Illumina ® MiSeq ® (2 x 75 bases). (neb.com)
ezRAD libraries were generated by digesting samples with the isoschizomer restriction enzymes MboI and Sau3AI (New England BioLab), which cleave at GATC cut sites, and libraries were generated with the KAPA HyperPrep Kit (Roche) using TruSeq DNA indexes (Illumina). (bco-dmo.org)
Genomic DNA was used to construct Ovation Ultralow libraries for Illumina sequencing. (cdc.gov)
If you have more specific questions regarding preparing the library pools for loading on the requested sequencer, please contact Illumina tech support . (lu.se)
Your libraries need to be compatible with requested Illumina platform. (lu.se)
Illumina has guidelines about library loading concentration, but these often need optimization. (lu.se)
If library preparation kit is not manufactured by Illumina, please check with the kit producer. (lu.se)

The comparison between the direct and fosmids sequencing revealed a bias in the fosmid libraries against low-GC DNA and specifically against the two most dominant members of the community, Candidatus Pelagibacter and P. marinus subsp. (nih.gov)
Genomic fosmid libraries were constructed for the BDGP by Andreas Gnirke at Exelixis, Inc. (South San Francisco) from Drosophila erecta, D. pseudoobscura, D. virilis, and D. willistoni. (fruitfly.org)

My work contributes to the important and growing field of cnidarian evolutionary genomics and hopefully paves the way for future evolutionary genomic work on these and other intriguing cnidarian systems. (ku.edu)

and a cDNA library, which represents the mRNA from a cell or tissue at a specific point of time. (biologydiscussion.com)
The choice of vector also depends on whether a genomic library or cDNA library is constructed. (biologydiscussion.com)
A cDNA library was constructed from a thymus from a five-week-old BALB/c mouse. (caltech.edu)

In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. (wikipedia.org)
The vector containing the inserted fragments of genomic DNA can then be introduced into a host organism. (wikipedia.org)
This creates fragments that are similar in size, each containing one or more genes. (wikipedia.org)
pastoris, thus unexpectedly providing a feasible method to obtain large genomic fragments from other less prevalent members of this community. (nih.gov)
A DNA library is a set of cloned fragments that collectively represent the genes of a particular organism. (biologydiscussion.com)
By digesting complex genomic DNA from an organism it is possible to reproducibly divide its genome into a large number of small fragments, each approximately the size of a single gene. (biologydiscussion.com)
This means that digesting genomic DNA with EcoRI, which recognizes the sequence 5′-GAATTC-3′, will produce fragments each of which is, on average, just over 4 kb. (biologydiscussion.com)
Adaptors were ligated to DNA fragments, which were subsequently size selected (~300bp). (nih.gov)
For ChIP-Seq, the 36 bp sequence reads were directionally extended to 300 bp, corresponding to the length of the original fragments used for sequencing, and tags mapping on exact the same genomic locus were discarded to obtain a non-redundant set. (nih.gov)
The template or genomic DNA is fragmented using the restriction enzyme into smaller-sized fragments and ligated to the known DNA adapters. (geneticeducation.co.in)
Partial EcoRI and randomly sheared fragments, average insert size 152 and 156 kb, respectively. (fruitfly.org)

Human PBMC Genomic DNA was prepared from purified healthy human peripheral blood mononuclear cells (PBMCs) of single donor. (3hbiomedical.com)

In my dissertation, I use phylogenetic, genomic, and population genetic approaches to study genome-scale and population-level changes associated with the evolution of major life history transitions. (ku.edu)

The high abundance of phage sequences in the cellular size fraction indicated a remarkably high proportion of cells suffering phage lytic attack. (nih.gov)
Dataset: Genetic sequence accessions, collection information, and methodology for raw sequences from ezRAD libraries of Pocillopora spp. (bco-dmo.org)
These sequences were analyzed for size variation using the polymerase chain reaction and gel electrophoresis without the need for radiolabeling. (ox.ac.uk)
By using next generation sequencing, genomic sequences can be obtained and compared to identify potential genotyping markers. (cdc.gov)
MiSeq sequencing reads were taxonomically profiled for contamination, de novo assembled, and mapped to a draft genome available in GenBank to assess the quality of the resulting genomic sequences. (cdc.gov)
The methods described here will facilitate the accumulation of genomic sequences from various samples, which is a prerequisite for the development of typing tools to aid in outbreak investigations. (cdc.gov)

In Chapter 2, I investigate the cnidarian transition to endoparasitism by contributing to phylogenomic analyses to place these parasites within the cnidaria, and characterizing major genome-scale changes, such as gene loss and genome size change, as compared with other non-parasitic cnidarians. (ku.edu)
Estimates of genome size based on k-mer frequency analysis were highly similar to the total size of the finished genome, suggesting that the entirety of the genome was recovered. (authorea.com)

Genomic DNA is extracted from plant leaves and subjected for treatment to remove contaminant RNA, protein and polysaccharide, Genomic DNA is measured by Nanodrop (a spectrophotometer technology, not by fluorometer/Qubit) and provided in 1.5-ml tubes at a concentration of 0.5mg/ml or 1mg/ml in TE buffer. (delos.info)

An organism's genomic DNA is extracted and then digested with a restriction enzyme. (wikipedia.org)
The small size of this active enzymatic domain and its temperature stability suggest that it may be of value in the enzyme-enhanced bleaching of kraft pulp. (edu.au)

These are due to the advance of genome-wide association studies from the ability to create and sequence genomic libraries. (wikipedia.org)
The principal aim of this study was to address this knowledge gap and to assess predictive ability of genomic Best Linear Unbiased Prediction (gBLUP) for traits of commercial importance in a yellowtail kingfish population comprising 752 individuals that had DNA sequence and phenotypic records for growth traits (body weight, fork length and condition index). (biomedcentral.com)
To further pursue the characterization of the Thy19.4 gene, a genomic clone containing this gene was isolated from a size-selected insert library, and the DNA sequence of the Thy19.4 gene was obtained. (caltech.edu)

Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis. (wikipedia.org)
Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. (wikipedia.org)
Researchers can choose a vector also considering the ideal insert size to find the desired number of clones necessary for full genome coverage. (wikipedia.org)
After a genomic library is constructed with a viral vector, such as lambda phage, the titer of the library can be determined. (wikipedia.org)
Cosmid vector for genomic library construction. (addgene.org)

We undertook an integrated analysis of genomic and epidemiological data to investigate a large health-care- associated outbreak of coronavirus disease 2019 (COVID-19) and to better understand the epidemiology of COVID-19 cases in Tasmania, Australia. (who.int)
Genomic clusters were determined and integrated with epidemiological data to provide additional information. (who.int)
Genomic analysis provided useful additional information on COVID-19 in Tasmania, including evidence of a large health-care-associated outbreak linked to an overseas cruise, the probable source of infection in cases with no previously identified epidemiological link and confirmation that there was no identified community transmission from other imported cases. (who.int)

Construction of a genomic library involves creating many recombinant DNA molecules. (wikipedia.org)
These recombinant molecules are taken up by a host bacterium by transformation, creating a DNA library. (wikipedia.org)

To further investigate the genomic diversity among this group and to help characterize lineages of the plague organism that have no sequenced members, we present here the genomes of two isolates of the "classical" antiqua biovar, strains Antiqua and Nepal516. (asm.org)
Since the genome of an organism is fixed, chromosomal DNA may be isolated from almost any cell type in order to prepare genomic DNA. (biologydiscussion.com)
Similar to DArT methods based on array hybridisations, the technology is optimized for each organism and application by selecting the most appropriate complexity reduction method (both the size of the representation and the fraction of a genome selected for assays) and it can generate a large number of markers at a reasonable price per sample (approx. (biomedcentral.com)

Zyagen offers a pure and intact high molecular size control genomic DNA of 32 different plant species. (delos.info)
Along with the conventional selective breeding approach based on phenotype and pedigree, we are interested in exploring potential use of molecular and genomic information to accelerate genetic gain in this line of YTK, especially improving traits that are difficult or expensive to measure or lowly heritable. (biomedcentral.com)

Genomic prediction using Diversity Arrays Technology (DArT) genotype by sequencing platform has not been reported in yellowtail kingfish ( Seriola lalandi ). (biomedcentral.com)

Collectively our results demonstrated, for the first time in yellowtail kingfish, the potential application of genomic selection for growth-related traits in the future breeding program for this species, S. lalandi . (biomedcentral.com)
In a new study, an international research team found that this genetic colour variation that is shared between several species arose through changes in a specific genomic region at least five million years ago. (lu.se)

CF27 exhibits a large number of genomic islands (mostly containing genes of unknown function), suggesting that a large number of genes has been acquired by horizontal gene transfer over time. (frontiersin.org)
Particular genes can be isolated from DNA libraries, much as books can be obtained from conventional libraries. (biologydiscussion.com)
From this library, 69 class I cDNA transcripts from 15 different class I genes were isolated and analyzed. (caltech.edu)

Presence of correctly-sized bands were checked on an E-Gel electrophoresis machine. (cdc.gov)
Perform quality and size calculation of libraries on a micro-capillary gel electrophoresis system e.g. (lu.se)

After a final PCR amplification step (18 cycles), the resulting DNA libraries were quantified and sequenced on HiSeq 2000. (nih.gov)
over-amplification during library preparation can produce inefficient, inaccurate or biased results. (geneticeducation.co.in)

We have developed a method for the assessment of genomic copy number profiling data, and suggest to apply the method in addition to and after other state-of-the-art noise correction and quality control procedures. (uzh.ch)

The genomic DNA was treated with RNase A, dissolved in buffer and frozen immediately. (3hbiomedical.com)
Dilute and pool your libraries in EB buffer (10mM Tris-HCl, pH 8.5). (lu.se)

Genomic insights are an important component of the response to COVID-19, and continuing genomic surveillance is warranted. (who.int)

Nanopore MinION device at Instituto Nacional Investigação em Saúde (INIS), Ministry of Health of Angola, Luanda, on the AO-1 isolate, as part of the ArboSPREAD project focused on genomic surveillance of arthropod-borne viruses. (cdc.gov)

Colonies containing AG, AC, and ATG repeats were selected from enriched genomic libraries of Douglas-fir, and 603 were sequenced. (psu.edu)

abstract = "A genomic library of the extremely thermophilic eubacterial strain Rt8B.4 was constructed in λZapII and screened for the expression of xylanase activity. (edu.au)

Altered segments ranged in size from those involving a single clone to regions as large as 14 Mb. (bmj.com)

Lysates were cleared by centrifugation and stored at -80 C. Genomic DNA (Input) was prepared by treating aliquots of chromatin with RNaseA, proteinase K and heat for de-crosslinking, followed by phenol/chloroform extraction and ethanol precipitation. (nih.gov)

In all populations of limited size, genetic variation is lost over time. (lu.se)

We used reduced representation genomic libraries to infer the role of neutral processes (drift and restricted gene flow) and divergent selection in driving variation in thermoregulatory traits with an emphasis on the mechanisms that maintain bill divergence among islands. (nsf.gov)
Collectively, these results suggest strong divergent selection across an island archipelago results in genomic changes in a suite of traits associated with climate adaptation over small spatial scales. (nsf.gov)

Human Genomic DNA is sourced from whole blood that has tested negative for HIV antibodies and Hepatitis B surface antigen. (takarabio.com)

CNARA could be instrumental in improving the assessment of data used for genomic data mining experiments and support the reliable functional attribution of copy number aberrations especially in cancer research. (uzh.ch)

1976), and is suitable for Southern hybridization analysis and genomic library construction. (takarabio.com)
Thus, it is important to consider carefully the cell or tissue type from which the mRNA is to be deriver in the construction of cDNA libraries. (biologydiscussion.com)

This allows researchers to also determine the percentage of infectious viral particles actually carrying a fragment of the library. (wikipedia.org)
Aligns were extended in silico at their 3'-ends to a length of 200 bp, which is the average genomic fragment length in the size-selected library, and assigned to 32-nt bins along the genome. (nih.gov)
Peak locations were determined using the SICER algorithm (v1.1) with a cutoff of FDR = 1E-10 and a Gap size of 3x fragment size (200 bp) for H3K27me3 and 1x fragment size for H3K4me3. (nih.gov)
The choice of the particular type of gene library depends on a number of factors, the most important being the final application of any DNA fragment derived from the library. (biologydiscussion.com)
Every fragment that we are going to add to the library must be amplified almost evenly. (geneticeducation.co.in)
In addition, preparing a multiplex PCR reaction or setting up different PCR for every fragment from the library is also not possible, realistically. (geneticeducation.co.in)

EC 2.1.1.6) was determined from rat cDNA and genomic libraries were screened with DNA probes and specific antiserum. (lu.se)

It was used technique of documentation based on literature available at Scielo (Scientific Electronic Library Online) and MEDLINE between 2000 and 2010. (bvsalud.org)

However, if the goal is the production of new or modified proteins, or the determination of tissue-specific expression of timing patterns, cDNA libraries are more appropriate. (biologydiscussion.com)
Genomic DNA was extracted from tissue using the OMEGA (BIO-TEK) E.Z.N.A. Tissue DNA Kit. (bco-dmo.org)

Genomic libraries are commonly used for sequencing applications. (wikipedia.org)
Sanger and his team of scientists created a library of the bacteriophage, phi X 174, for use in DNA sequencing. (wikipedia.org)
The library was loaded onto a flow cell (FLO-MIN106) and sequencing data were collected for 24 hours. (cdc.gov)
Conclusions Results suggested that the NGS approach based on AmpliSeq™ libraries and Ion Personal Genome Machine (PGM) sequencing is a highly efficient mutation detection method. (researchgate.net)
Genotyping by sequencing (GBS) has been used as an alternative to whole genome sequencing approach due to its low cost of genotyping per animal, large amount of high quality genetic markers and the suitability of the markers for genomic prediction/selection. (biomedcentral.com)
CTG provides service for sequencing of user-prepared libraries on NovaSeq 6000 and NextSeq2000. (lu.se)
However, as the libraries are not prepared at CTG for these types of projects we cannot guarantee the output of the sequencing run. (lu.se)
For the sequencing recommendations, we refer you to the library preparation kit manufacturer. (lu.se)
Optimal pool loading concentration depends on the library type, insert size and is specific to the sequencing machine and kit. (lu.se)
In Australia, integration of genomic sequencing METHODS into the response to COVID-19 has al owed clusters and outbreaks to be identified and transmission chains to COVID-19 cases notified to the Tasmanian Department of be rapidly detected. (who.int)

Below are the steps for creating a genomic library from a large genome. (wikipedia.org)
Here, we propose a method to distinguish reliable genomic copy number profiles from those containing heavy wave artefacts and/or large DLRS. (uzh.ch)
Analyses of 22,029 neutral SNPs confirm distinct population structure by island with restricted gene flow and relatively large effective population sizes, suggesting bill differences are probably not a product of genetic drift. (nsf.gov)

Thus, conventional PCR can't perform library enrichment for NGS, effectively. (geneticeducation.co.in)

Data showed consistent GC coverage (A) and insert size (B). 2 million paired-end reads from each library were sampled (seqtk v1.3), adapter-trimmed (seqprep v0.1) and mapped to the GRCh38 reference (bowtie2 v2.4.5), and GC coverage information was calculated using Picard's CollectGCBiasMetrics (v 1.56.0). (neb.com)
9 Genomic data enhance national Health between 2 March and 15 May 2020 were included surveil ance data by clarifying the source of infection in in the analysis. (who.int)

We screened a mouse BAC library with this cDNA to isolate the IGRP gene, which spans approximately 8 kbp of genomic DNA. (diabetesjournals.org)

In under 2 hours, the protocol enables the creation of high-yield, high-quality libraries, from a broad input amount range, while generating less plastic waste. (neb.com)
Further, with simplicity at the forefront, the kit features a single protocol for all input amounts, making NEBNext UltraExpress DNA Library Prep well suited for all throughputs. (neb.com)
Responding to user need for a faster, streamlined DNA prep workflow that delivers high quality libraries from a variety of sample types, the NEBNext UltraExpress DNA Library Prep Kit has a single protocol for all DNA inputs (10 - 200 ng of pre-sheared DNA). (neb.com)
Libraries were prepared using a single protocol from cell-free DNA (cfDNA, 12 ng) without shearing and Maize DNA (10 ng and 100 ng) sheared to 200bp (Covaris ® ME220). (neb.com)

A similar method can be used to titer genomic libraries made with non-viral vectors, such as plasmids and BACs. (wikipedia.org)
This genomic DNA is isolated by the method of Blin and Stafford (Blin, et al. (takarabio.com)

I use phylogenomic and population genomic techniques in order to study the relationship between salinity level and population structure, reconstruct the evolution of salinity tolerance within Cordylophora, and to clarify some taxonomic uncertainty within the genus. (ku.edu)

Genomic and phenotypic analysis of one specific lncRNA demonstrate its importance in sexual differentiation and reproduction. (bvsalud.org)
4-6 Locally, genomic shown to be beneficial in investigating health-care- analysis has been used to analyse transmission routes, associated infections, 9,17 monitoring community trans- assign likely origins of infection, link outbreak cases and mission, 8-10 informing public health responses 9,10,18 and inform public health interventions and policies. (who.int)

This high-quality, highly pure genomic DNA is isolated from the whole blood of disease-free sources that contain no glycogen. (takarabio.com)
Extensive quality control procedures ensure high quality genomic DNA. (3hbiomedical.com)

Anatomy9

Organisms29

Diseases3

Chemicals and Drugs59

Analytical, Diagnostic and Therapeutic Techniques and Equipment32

Phenomena and Processes109

Disciplines and Occupations2

Anthropology, Education, Sociology and Social Phenomena2

Technology, Industry, Agriculture6

Information Science29

Named Groups1

Health Care5

Purity of genomic1

Total genomic2

Illumina8

Fosmid libraries2

Evolutionary1

CDNA library3

Fragments11

Single donor1

Phylogenetic1

Sequences6

Genome Size2

Polysaccharide1

Enzyme2

Sequence3

Vector5

Epidemiological3

Recombinant2

Organism3

Molecular2

Genotype1

Species2

Genes3

Electrophoresis2

Amplification2

Profiling1

Buffer2

Insights1

Surveillance1

Repeats1

Abstract1

Clone1

Centrifugation1

Genetic Variation1

Traits2

Antibodies1

Experiments1

Construction2

Fragment6

Probes1

20001

Tissue2

Sequencing10

Large3

Effectively1

Data2

Isolate1

Protocol4

Method2

Order1

Analysis2

High-quality2