Site recombinant fusion proteins. Medical search. Frequent questions

Site-directed mutagenesis was used to selectively eliminate the IgG binding for each epitope and single and multiple mutations were expressed as recombinant GST fusion proteins. (cdc.gov)
Sequential methionine substitution by SPOTsalogue identified K68, E122, and K131 as critical aa in each epitope to change by site-directed mutagenesis. (cdc.gov)

In molecular biology, recombinant proteins are frequently expressed fused to other proteins ("carrier"Â proteins), to correct purification. (venomsupplies.com)
Vectors containing the preprotrypsin leader (PPT) sequence direct secretion of FLAG fusion proteins into the culture medium for purification using ANTI-FLAG antibodies, resins, and plates. (sigmaaldrich.com)
Rentschler employs cutting-edge technologies to ensure safe and stable cell culture and protein purification processes. (pharmaceutical-business-review.com)
Rentschler delivers upstream process (USP) development for cell cultivation and downstream process (DSP) development for protein purification as well as formulation development. (pharmaceutical-business-review.com)
Result: The purification of the TM domain of p75 neurotrophin receptor using a KSI tag with the introduction of a thrombin cleavage site is described herein. (a-star.edu.sg)
The completed gene DNA sequence coded for Neurotensin and green fluorescence proteins (GFP) where additional iterations were required to add a polyhistidine-tag, HIS-tag, for Nickel column recombinant protein purification. (nanomedic.org)
However, one of the factors that make it difficult and raise the price of production in every system consists in the purification process of the recombinant antigen. (scielo.br)
In addition, DDDDK is a part of the octapeptide FLAG tag (DYKDDDDK), which can be utilized as a fusion tag for recognition by antibody, and for detection of fusion protein expression with Western blot analysis, as well as for purification of the fusion protein by Anti-FLAG affinity chromatography. (abmgood.com)

To improve site-specific cleavage of a methionyl porcine growth hormone [[Met1]-pGH(1-46)-IGF-II] fusion protein by the enzyme H64A subtilisin, a series of flexible, unstructured spacer peptides were introduced N-terminal to the cleavage site. (edu.au)
Extracellular domain with 29aa N-terminal T7-His-TEV cleavage site tag (NP_002600). (abcam.com)
Like the original FLAG tag, 3xFLAG ® is hydrophilic, contains an Enterokinase cleavage site, and is relatively small. (sigmaaldrich.com)
The additional PreScission protease cleavage site allows the removal of the GST-tag. (neuromics.com)
Beads for efficient and convenient cleavage of recombinant fusion proteins containing plasmin-specific cleavage site. (biovisi.com)
A particle according to any one of the preceding claims, wherein the protease cleavage site is accessible to the relevant protease (i.e. that which recognises the cleavage site) before the viral particle becomes bound to an eukaryotic cell. (sumobrain.com)
A particle according to any one of claims 1 to 9, wherein the protease cleavage site becomes accessible to die relevant protease only after the viral particle has become bound to an eukaryotic cell. (sumobrain.com)
A particle acording to claim 11 , wherein the protease cleavage site becomes accessible after the heterologous polypeptide has bound to its cognate receptor on the eukaryotic cell. (sumobrain.com)

I. High resolution epitope mapping and characterization of monoclonal antibody binding sites. (duke.edu)
Antibody recognition of the mutant proteins was determined by inhibition ELISA. (cdc.gov)
T-VIVA-19 is a recombinant fusion protein of the spike protein S1-domain and the Fc portion of the human IgG1 antibody (rS1-Fc). (globenewswire.com)
Unlike other vaccine candidates, our rS1-Fc protein production could utilize our existing therapeutic antibody manufacturing processes, and we therefore believe it would be simple and easy to scale up. (globenewswire.com)
Oncotarget recently published " A novel format for recombinant antibody-interleukin-2 fusion proteins exhibits superior tumor-targeting properties in vivo which reported that here, the authors describe four novel formats for the L19-IL2 fusion, featuring different arrangements of antibody and IL2. (oncotarget.com)
Based on ratiometric analysis of these diffraction images acquired at the detector-array, we demonstrate the proof-of-concept of this biosensor by monitoring in real-time biomolecular interactions of protein A/G with immunoglobulin G (IgG) antibody. (nature.com)
Monoclonal antibody therapy is a form of immunotherapy that uses monoclonal antibodies (mAbs) to bind monospecifically to certain cells or proteins. (wikimili.com)
We show that the S1-Fc fusion protein is extraordinarily immunogenic, as evidenced by robust antibody titers noticed by day 7. (genoimmun.com)
To minimize the problems due to use of pure mouse antibody, researchers have used recombinant DNA techniques to create monoclonal antibodies that are part human and part mouse. (msdmanuals.com)

Assay conditions: The recombinant fusion protein is dissolved in the digestion buffer (20 mM Tris-HCl, 100 mM NaCl, 2 mM CaCl2, pH 8.0). (venomsupplies.com)
The C-terminal domain of the 990-amino acid Schizosaccharomyces pombe Spt5 protein, composed of repeats of a nonapeptide motif (consensus sequence TPAWNSGSK), is necessary and sufficient for binding to the capping enzymes in vivo (in a two-hybrid assay) and in vitro. (mskcc.org)
b) Schematic drawing of a bioanalytical REA assay before (left) and after (right) binding of AuNP-analyte complexes to the capture protein layer. (hindawi.com)
Using an in vitro protein-protein interaction assay, we demonstrate that the two intracellular domains of Kir6.2 physically interact with each other, and we map a region within the N terminus that is responsible for this interaction. (ox.ac.uk)
Sturdy virus neutralizing exercise was noticed on day 14 in rabbits immunized with the S1-Fc fusion protein utilizing a pseudovirus neutralization assay. (genoimmun.com)
Recombinant Immunoblot Assay (RIBA) was used as a confirmatory assay for anti-HCV reactive samples but was discontinued in 2013. (cdc.gov)
We report the development of an anti-HCV assay performed on an automated immunoblot platform using a fourth generation HCV recombinant fusion protein. (cdc.gov)

The designer protein combines the binding site for two cytokines that regulate maturation, function, and survival of B cells - B-lymphocyte stimulator (BLyS) and A proliferation-inducing ligand (APRIL), with the constant region of immunoglobin. (wikipedia.org)
Additionally, the application of biological processes involving recombinant DNA technology, which allowed the production of proteins like cytokines and humanized antibodies, must be credited ( Mazurek and Jahnz-Rozyk, 2012 ). (frontiersin.org)
Focused on mammalian cell lines, our experience covers the development and manufacturing of recombinant proteins such as monoclonal antibodies, fusion proteins, cytokines and enzymes in compliance with international GMP standards (EMA/FDA). (pharmaceutical-business-review.com)

Production of perdeuterated proteins for neutron-scattering experiments. (lu.se)

Antibodies to ribosomal P2 protein were rarely detected, ransmission routes of human parvovirus 4 (PARV4), except in patients with systemic lupus erythematosus ( 5 ). (cdc.gov)
Recombinant SARS-CoV-2 antigens, such as the Spike (S) glycoprotein or Nucleocapsid (N), are used for the isolation of target-specific neutralizing antibodies. (invivogen.com)
We used a series of COOH-terminally deleted recombinant myosin molecules to map precisely the binding sites of 22 monoclonal antibodies along the tail of Acanthamoeba myosin-II. (duke.edu)
Most of the antibodies that bind to the distal 37 nm of the tail disrupt assembly of octameric minifilaments and, depending upon the exact location of the binding site, stop assembly at specific steps yielding, for example, monomers, antiparallel dimers, parallel dimers or antiparallel tetramers. (duke.edu)
The effects of these antibodies on assembly identify sites on the tail that are required for individual steps in minifilament assembly. (duke.edu)
The antibodies did not appear to recognize the epitope 63-68 in the recombinant fusion protein. (cdc.gov)
Immunization with the rS1-Fc protein via intramuscular and intravenous injections induced antibodies against the SARS-CoV-2 protein in all mice within the first week of administration. (globenewswire.com)
Disclosed herein are neutralizing monoclonal antibodies (MAbs) specific for the surface hemagglutinin (HA) protein of the influenza H5N1 strain. (justia.com)

272-630 and aa 604-914 of ORF2, were fused to bacterial and Chien-Ching Hung small ubiquitin-like modifi er (SUMO) protein (a member of a ubiquitin-like protein family) and used as antigens in In studying the epidemiology of parvovirus 4 (PARV4) immunoblot. (cdc.gov)
The obtainment of transgenic edible plants carrying recombinant antigens is a desired issue in search for economic alternatives viewing vaccine production. (scielo.br)
Small synthetic peptides that mimic surface antigens of pathogens and are immunogenic, or vaccines manufactured with the aid of recombinant DNA techniques. (bvsalud.org)

InvivoGen offers a collection of pFUSE-Fc plasmids developed to facilitate the expression and production of Fc-Fusion proteins, by fusing a sequence encoding a given protein to the Fc region of an immunoglobulin (Ig). (invivogen.com)
One of their specialties is the production of unique immunoglobulin based chimeric fusion proteins using advanced cellular and molecular biological techniques. (bio-connect.nl)

Cleavage sites for sequence-specific proteinases are engineered between the leader protein and the recombinant protein. (venomsupplies.com)
This led us to construct recombinant DNA plasmids containing the encoding sequence of HBsAg and the same construction fused with the SEQDEL sequence responsible for ER sorting. (scielo.br)
It is highly specific for cleaving fusion proteins with the recognition sequence, Asp-Asp-Asp-Asp-Lys (DDDDK). (abmgood.com)
Unlike other site-specific proteases that cut within the recognition sequences leaving extra amino acids in the cleaved peptide products, the EK1 cleaves after the C-terminal end of the lysine residue, and the fragment produced from the cleavage reaction does not inherit any residues from the DDDDK recognition sequence. (abmgood.com)
Therefore, the application can be extremely advantageous for producing a 100% native protein sequence and structure from recombinant fusion protein, which has the desired product immediately after the enterokinase recognition sequence, DDDDK. (abmgood.com)
The Enterokinase will also be able to cleave the tag if it is placed at the c-terminus, however please be aware that this will result in the recogition sequence being left intact on your protein of interest, even after the his tag has been removed. (abmgood.com)

When enzymatic digestion preceded refolding of the fusion proteins, IGF-II could only be liberated from substrates which contained spacer peptides. (edu.au)

Clotting factors work by supplying proteins missing in people with hemophilia. (myhemophiliateam.com)
Factor IX is the treatment of choice for acute hemorrhage or presumed acute hemorrhage in patients with hemophilia B. Recombinant factor IX is the preferred source for replacement therapy. (medscape.com)
Recombinant factor VIIa (rFVIIa) has become available for treating people with hemophilia with inhibitors who experience bleeding or require surgery. (medscape.com)

The coagulation of blood is maintained by cellular components and soluble plasma proteins. (medscape.com)
If the membrane-anchor is missing or defective, Fp and Ip subunits accumulate as soluble proteins in the cytoplasm. (lu.se)

Fc-fusion proteins are molecules consisting of an IgG-Fc domain fused to a protein of interest, such as an extracellular domain of a receptor, ligand, enzyme, or peptide. (invivogen.com)
The core substitution A71L impaired T immunogenicity of the target epitope within the protein, but not in the peptide, whereas the N73A substitution impaired the responses in both instances. (aber.ac.uk)
Their molecule is a recombinant, multi-functional fusion protein that has both a targeting peptide domain (tCRK peptide) for a delivery to normal skin and skin wounds and Decorin (DCN) anti-inflammatory and anti-fibrotic protein as an active therapeutic component. (tuni.fi)

The technical name of Alprolix is Coagulation factor IX (recombinant), Fc fusion protein. (myhemophiliateam.com)
The role of the coagulation system is to produce a stable fibrin clot at sites of injury. (medscape.com)

Bone Biologics, Inc. announced that it has successfully completed the initial primate trials validating the efficacy of the proprietary bone growth regeneration UCB-1 (NELL-1) protein in spinal fusion surgery. (medlatest.com)
Bone Biologics has been developing the protein as a Platform Technology since 2004, leveraging the previous ten years of research in the lab performed by the founding scientists at UCLA. (medlatest.com)
This Platform Technology is combined with DBX ® demineralized bone matrix to promote bone growth in spinal fusion. (medlatest.com)
Posterior lumbar interbody fusion using recombinant human bone morphogenetic protein type 2 with cylindrical interbody cages. (wakehealth.edu)
Meta-analysis of the Impact of Patient Characteristics on Estimates of Effectiveness and Harms of Recombinant Human Bone Morphogenetic Protein-2 in Lumbar Spinal Fusion. (medscape.com)
Augment bone graft products compare favorably with autologous bone graft in an ovine model of lumbar interbody spine fusion. (shengsci.com)
abstract： STUDY DESIGN:This study was designed to determine whether Augment Bone Graft (Augment, Biomimetic Therapeutics, Inc., Franklin, TN) and Augment Injectable Bone Graft (Augment Injectable, Biomimetic Therapeutics, Inc., Franklin, TN), 2 combination devices comprising recombinant human platelet-derived growth factor-BB an. (shengsci.com)
Improved understanding of spinal biomechanics, proliferation of sophisticated spinal instrumentation devices, advances in bone fusion techniques, refinement of anterior approaches to the spine, and development of microsurgical and minimally invasive methods have made it possible to stabilize every segment of the spine successfully, regardless of the offending pathology. (medscape.com)
It should be noted that whereas the term fusion, as used in this article and in spine literature to refer to the concept of internal stabilization of the spine, generally refers to fusion with instrumentation (instrumented fusion), such stabilization has also, albeit with decreasing frequency, been accomplished by means of bone grafting alone. (medscape.com)

A comparative quantitative biodistribution analysis in tumor-bearing mice using radioiodinated proteins revealed that the novel format exhibited the best biodistribution results. (oncotarget.com)

On this research, we examined the immunogenicity of CHO-expressed recombinant SARS-CoV-2 S1-Fc fusion protein in mice, rabbits, and monkeys as a possible candidate for a COVID-19 vaccine . (genoimmun.com)

The proper plasma membrane localization was confirmed by observing the fluorescence of the N-terminal yellow fluorescent protein tag. (mdc-berlin.de)

Inhibition ELISA with the mutant proteins indicated that epitope 126-131 was the dominant epitope, but mutation of epitope 120-125 was also required to eliminate mAb reactivity to Hev b 5. (cdc.gov)
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. (rndsystems.com)

Description: CDC37 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 378 amino acids and having a molecular mass of 44.4 kDa. (genoimmun.com)

How accurate are machine learning models at identifying the predictive factors for readmissions after posterior lumbar fusion procedures? (medscape.com)
Mini-open Anterior Lumbar Interbody Fusion for Recurrent Lumbar Disc Herniation Following Posterior Instrumentation. (medscape.com)
Effects of Intraoperative Anesthetic Medications on Postoperative Urinary Retention After Single-Level Lumbar Fusion. (medscape.com)
Modified Mini-open Transforaminal Lumbar Interbody Fusion: Description of Surgical Technique and Assessment of Free-hand Pedicle Screw Insertion. (medscape.com)
Predicting the outcome of selective thoracic fusion in false double major lumbar "C" cases with five- to twenty-four-year follow-up. (shengsci.com)
OBJECTIVE:To examine the long-term outcome of selective thoracic fusion (STF) performed for lumbar "C" modifier curves in adolescent idiopathic scoliosis. (shengsci.com)
Short-term studies revealed equivalent results for disk arthroplasty and lumbar fusion. (medscape.com)

The B7 family consists of structurally related cell-surface protein ligands, which bind to receptors on lymphocytes that regulate immune responses. (bio-connect.nl)

Since 1987 two recombinant vaccines produced into Saccharomices cerevisiae cells by Sharp & Dhome and Smith Kline Beechmam have been utilized for HBV immunization. (scielo.br)

Genetic transformation process was acquired by cocultivation of cotyledons with Agrobacterium tumefaciens harboring the recombinant plasmid. (scielo.br)

We offer native and recombinant proteins expressed and purified from bacterial, insect, and mammalian expression systems. (bio-connect.nl)

Etanercept is a recombinant human tumour necrosis factor (TNF) receptor p75Fc fusion protein. (bmj.com)
The strong human cytomegalovirus (CMV) promoter regulatory region drives constitutive protein expression levels as high as 1 mG/L in COS cells. (sigmaaldrich.com)
T-VIVA-19 ( t argeted vi rus va ccine against COVID- 19 ) is a recombinant fusion protein of the SARS-CoV-2 spike protein S1 domain and human IgG Fc. (globenewswire.com)
When Recombinant Human BTLA (Catalog # 9235-BT ) is immobilized at 1.00 µg/mL (100 µL/well), Biotinylated Recombinant Human HVEM/TNFRSF14 Fc Chimera Avi-tag binds with an ED 50 of 30.0-180 ng/mL. (rndsystems.com)
When Recombinant Human BTLA ( 9235-BT ) is immobilized at 1.00 µg/mL (100 µL/well), Biotinylated Recombinant Human HVEM/TNFRSF14 Fc Chimera Avi-tag binds with an ED 50 of 30.0-180 ng/mL. (rndsystems.com)
2 μg/lane of Biotinylated Recombinant Human HVEM/TNFRSF14 Fc Chimera Avi-tag (Catalog # AVI10726) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 61-67 kDa and 120-130 kDa, respectively. (rndsystems.com)

This has become the case for treatment of hemorrhage with recombinant factor VIIa (rFVIIa). (medscape.com)

Thus, striking improvements in recombinant antigen were achieved by alternative polyadenylation signals and fusion proteins containing targeting signals designed to enhance integration or retention of HBsAg in the endoplasmic reticulum (ER) of plant cells [6]. (scielo.br)

CQ resistance is associated with mutations in the CQ resistance transporter (PfCRT), a membrane protein of the digestive vacuole that allows the efflux of the drug form its site of action. (gla.ac.uk)
HVEM (herpesvirus entry mediator), also known as TNFRSF14 and CD270, is a type I membrane protein in the TNF receptor superfamily, and it can both promote and inhibit T cell activity (1). (rndsystems.com)
The flavin together with the iron-sulfur clusters function to mediate electron transfer from the dicarboxylate binding site on the Fp subunit to the membrane-anchor domain where quinone is reduced to quinol. (lu.se)

Our selection of CMV vectors provides options for transient or stable expression of 3xFLAG ® fusion proteins. (sigmaaldrich.com)

Detection of fusion proteins containing 3xFLAG ® is enhanced up to 200 times more than any other system. (sigmaaldrich.com)
Along these lines, we have recently introduced a handheld plasmonic biosensing device 12 that merges on-chip imaging and nanohole arrays towards detection of ultrathin protein layers, which might find use in field deployable sensing applications. (nature.com)

From our experience on some proteins we have, 1 kit (100ul) will cleave about 1mg of protein. (abmgood.com)

Scholars@Duke publication: Identification of functional regions on the tail of Acanthamoeba myosin-II using recombinant fusion proteins. (duke.edu)
In conclusion, immunological analysis of mutated proteins revealed functional associations between topographically distinct antigenic determinants which may account for the previously observed differences in the specificity of immune responses between immunised and infected hosts. (aber.ac.uk)
Increased ligand binding affinity was accompanied by a gain in calcium mobilization capability, suggesting functional coupling to G proteins. (unm.edu)

The course will be held in collaboration with the Lund Protein Production Platform, LP3 ( http://www.lu.se/lp3 ). (lu.se)

c-Myc transrepression is mediated by blockade of the transactivating activity of Miz-1, which occurs mainly through a Sp1-binding site in the proximal promoter of the gene. (aacrjournals.org)
HBsAg is the abundantly expressed envelope protein as a dimmer of two identical polypeptides encoded by the same gene. (scielo.br)

The expressed recombinant protein will be localized in the cytoplasm. (neuromics.com)

It is not generally necessary to deactivate the EK enzyme, however to prevent further degradation of the target protein after the reaction is finished, removal of enterokinase via affinity chromatography is recommended. (abmgood.com)

In rats, hepatic ketogenic capacity increases rapidly during postnatal development ( 4 ) or fasting ( 5 ) when the mRNA, protein, and activity of HMGCS2 increase in liver ( 6 - 8 ). (aacrjournals.org)
Administration of dibutyryl-cyclic AMP to adult rats increases mRNA, protein levels, and enzymatic activity of HMGCS2 in the liver ( 6, 9 ). (aacrjournals.org)

Additionally, recombinant SARS-CoV-2 Fc-fusion proteins are potential COVID-19 vaccine candidates. (invivogen.com)

They were named viral protein (VP) 2 and in Taiwan, we detected DNA in plasma of 3 mothers and their newborns with hydrops. (cdc.gov)
A particle according to any one of the preceding claims, wherein heterologous polypeptide sterically hinders fusion of an enveloped viral particle with an eukaryotic cell to which it is bound. (sumobrain.com)

The approximate protein concentration of Enterokinase G699 is 10ug/1ml. (abmgood.com)

Atacicept blocks activation of B cells by the tumor necrosis factor receptor superfamily member 13B (more commonly known as TACI), a transmembrane receptor protein found predominantly on the surface of B cells. (wikipedia.org)
Receptor that binds specifically to PDGFB and PDGFD and has a tyrosine-protein kinase activity. (abcam.com)

Studies showed that purified protein could be used for structural study using NMR spectroscopy. (a-star.edu.sg)
Conclusions: These results provide another strategy for obtaining a single TM domain for structural studies without using toxic chemical digestion or acid to remove the fusion tag. (a-star.edu.sg)

Furthermore, this cleavage rate was improved by removing a competing protease recognition motif from the fusion partner. (edu.au)
Enterokinase is used for site-specific cleavage of recombinant fusion proteins containing an accessible enterokinase recognition site for removal of affinity tags. (abmgood.com)
It is advisable to place the tag at the N-terminus of your protein in order to remove the recognition site along with the His Tag in full. (abmgood.com)

Proteinases which recognize these cleavage sites are used to cleave off the leader protein. (venomsupplies.com)
About 1 mg can be used to cleave 25 mg of protein). (venomsupplies.com)

Therefore, the risk of altering protein function, blocking other epitopes, or decreasing solubility is minimized. (sigmaaldrich.com)

Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. (rndsystems.com)

First, rigorous randomized controlled trials are needed to better assess the efficacy of existing methods of fusion. (medscape.com)

Site-specific and Quantitative N-glycan Heterogeneity Analysis of the Charge Isomers of an Anti-VEGF Recombinant Fusion Protein by High-resolution 2-dimensional Gel Electrophoresis and Mass Spectrometry. (electroforese.com)
Production of high-quality two-dimensional gel electrophoresis profile for marine medaka samples by using Trizol-based protein extraction approaches. (electroforese.com)

Anatomy10

Organisms11

Diseases1

Chemicals and Drugs41

Analytical, Diagnostic and Therapeutic Techniques and Equipment15

Phenomena and Processes28

Information Science3

Mutagenesis2

Purification8

Cleavage site8

Antibody9

Assay7

Cytokines3

Perdeuterated proteins for neutron-scattering1

Antibodies8

Antigens3

Immunoglobulin2

Sequence6

Peptides1

Hemophilia3

Soluble2

Peptide3

Coagulation2

Bone9

Radioiodinated proteins revealed1

Mice1

Fluorescence1

ELISA2

Amino acids1

Lumbar7

Receptors1

Vaccines1

Plasmid1

Bacterial1

Human6

Hemorrhage1

Signals1

Membrane3

Vectors1

Detection2

100ul1

Functional3

Lund Protein Production Platform1

Gene2

Cytoplasm1

Enzyme1

Rats2

Additionally1

Viral2

10ug1

Receptor2

Structural2

Recognition3

Cleave2

Epitopes1

Shelf Life1

Efficacy1

Electrophoresis2