[Show more] their performance in the Transcriptional Activator-Facilitated Enhancer trap (TAFET) constructs to induce cell- or tissue-specific expression patterns in rice. (edu.au)
The GAL4/VP16 TAFET system was able to reveal varying levels of tissue specific expression patterns in rice in the vegetative and generative tissues, and the expression patterns were proven to be due to the activity of the GAL4/VP16 transcriptional activator. (edu.au)
Specifically, as they wanted to analyze this interaction in human cells, they decided to utilize nuclease-inactive dCas9-based transcriptional activators where endogenous gene activation can serve as a sensitive measure of dCas9 binding to target DNA. (genomeweb.com)
Transcriptional activator Islet-Brain 1 (IB1) is disclosed which is involved in the control of the GLUT2 and insulin genes by interacting with homologous cis-regulatory elements of the GLUT2 and insulin promoters, and to materials and methods deriving from this work. (justia.com)
Although the current generation of dCas9-based transcription activators is able to achieve up-regulation of some endogenous loci, the magnitude of transcriptional up-regulation achieved by individual single-guide RNAs (sgRNAs) 12 typically ranges from low to ineffective 8 - 11 . (cdc.gov)
Tetracycline-controlled transactivator2
To limit the effect of the transgenes to the embryonic stage, promoter/enhancers (P/Es) from cellularization-specifically-expressed genes drive the expression of the tetracycline-controlled transactivator ( tTA ). (biomedcentral.com)
rTg4510 mice ("r" for regulatable) are produced by crossing the 4510 responder line, carrying a human MAPT P301L cDNA downstream of a tetracycline operon-responsive element (TRE), to an activator line expressing a tetracycline-controlled transactivator (tTA) under control of the CaMKIIα promoter. (alzforum.org)
Transcription activators1
In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. (abnova.com)
Gene11
Most of the TAFET lines examined showed reporter gene expression in many tissues. (edu.au)
The researchers also showed that hp-sgRNAs increased the specificity of gene editing using five different Cas9 or Cas12a variants, demonstrating that RNA secondary structures can tune the activity of diverse CRISPR systems. (genomeweb.com)
Transfecting sgRNA variants and a dCas9-P300 transactivator into human cells, we observed that hp-sgRNAs can tune gene activation at the target locus, suggesting modulation of dCas9 binding. (genomeweb.com)
9 , 10 In addition, chemically modified Cas12a guided-RNA has also been shown to facilitate improved gene correction in mammalian cells through both viral-and non-viral methods compared to the wild-type guide RNA. (biorxiv.org)
Methylation of class II transactivator gene promoter IV is not associated with susceptibility to multiple sclerosis. (ox.ac.uk)
The MHC class II transactivator (MHC2TA) is the master controller of expression of class II genes, and methylation of the promoter of this gene has been previously been shown to alter its function. (ox.ac.uk)
Characterization of the chromosomal rearrangements shown in Figure 1 also revealed that two genetic elements (proximal and distal MES [maintenance elements for silencing]), about 70 kb apart in the Scr gene, must be in cis to maintain proper repression. (nih.gov)
Our study shows the role of the CTGF gene as a target of miR-18a, and identifies the function of HBV/HBX/miR-18a/CTGF as a key signaling pathway mediating HBV infection-induced HCC. (medscimonit.com)
The expressed heterologous transactivator then activates the expression of the lethal effector gene hid Ala 5 [ 7 ] and leads to complete embryonic lethality in D. melanogaster . (biomedcentral.com)
These dCas9-activator fusions targeted to the promoter region of endogenous genes can then modulate gene expression 7 - 11 . (cdc.gov)
Cyclic AMP receptor protein was originally identified as a catabolite gene activator protein. (bvsalud.org)
Protein5
The ZEBRA (Z EBV replication activator) protein is the major transcription factor of EBV, expressed upon EBV lytic cycle activation. (mdpi.com)
In response to interferon (IFN), this protein forms a complex with STAT1 and IFN regulatory factor family protein p48 (ISGF3G), in which this protein acts as a transactivator, but lacks the ability to bind DNA directly. (abnova.com)
Transcription adaptor P300/CBP (EP300/CREBBP) has been shown to interact specifically with this protein, which is thought to be involved in the process of blocking IFN-alpha response by adenovirus. (abnova.com)
Electrophoretic mobility shift assays were performed in order to examine nuclear factor (NF)-κB and activator protein (AP)-1 binding activities. (ersjournals.com)
Translocator protein (TSPO), an 18 kDa mitochondrial protein, has been shown to be increased via PET imaging studies in HIV+ patients, in association with cognitive dysfunction. (uab.edu)
Endogenous2
While these transgenic assays have been important in defining the structure of the cis -regulatory elements and identifying trans -acting factors that bind to them, their functions within the context of the endogenous genes remain poorly understood. (nih.gov)
In addition, shrinkage of the dentate gyrus occurs in the CaMKIIα-tTA activator line, although it is not yet known whether this is due to the expression of tTA or to the disruption of one or more endogenous mouse genes. (alzforum.org)
Genetic1
These results collectively demonstrate the potential of Cas9-based activators as a powerful genetic perturbation technology. (cdc.gov)
Promoter1
A fragment of the murine GLUT2 promoter has been cloned and shown to be glucose-responsive when transfected into differentiated insulin-producing cells or into hepatocytes (35,36,52). (justia.com)
Receptors1
Normal neuromuscular junction showing a presynaptic terminal with a motor nerve ending in an enlargement (bouton terminale): Synaptic cleft and postsynaptic membrane with multiple folds and embedded with several acetylcholine receptors. (medscape.com)
Transgene2
A first approach to cause reproductive sterility by transgene-based embryonic lethality without the need of radiation has been successfully shown in the non-pest insect Drosophila melanogaster [ 6 ]. (biomedcentral.com)
T2 mice were then crossed with the CaMKIIα-tTA activator line to create bi-transgenic mice (designated rT2/T2 when homozygous for the MAPT P301L transgene). (alzforum.org)
Insertions1
The transcription units are shown above the genomic DNA, while chromosomal aberrations are shown below (solid triangles indicate insertions of transposable elements and upward arrows indicate breakpoints of translocations and inversions). (nih.gov)
Catalase2
The supplementation of Trans-resveratrol attenuated the cardiac oxidative stress and increased the activity of catalase. (bvsalud.org)
A suplementação com trans-resveratrol atenuou o estresse oxidativo cardíaco e aumentou a atividade enzimática de defesa catalase. (bvsalud.org)
Bind1
GTI and GTIII have been previously shown to bind distinct, but ubiquitously expressed trans-acting factors. (justia.com)
Regulate2
The changes provide powerful assays for identifying the trans -acting factors that regulate the homeotic genes and the cis -acting sequences through which they act. (nih.gov)
It was subsequently shown to regulate several functions unrelated to catabolism, and to be both a negative and a positive regulator of transcription. (bvsalud.org)
Reporter2
When combined with a FRET-based reporter, a fluorophore connected to a quencher via a short oligonucleotide sequence, the presence of the target activator can be confirmed. (biorxiv.org)
This phenomenon has been efficiently harnessed by SHERLOCK (Specific High-sensitivity Enzymatic Reporter unLOCKing) and DETECTR (DNA Endonuclease Targeted CRISPR Trans Reporter) to reliably detect nucleic acids. (biorxiv.org)
Mitochondrial1
NAD+ has been shown to play an important role in mitochondrial functioning. (fitnessbrain.co.uk)
Expression2
Patterns shown often in the vascular bundle, pericycle layer of cells in the root suggest that the CaMV35S enhancers affected the expression patterns in numbers of lines. (edu.au)
These observations are critical since several experimental models of diabetes have shown that GLUT2 expression is dramatically reduced specifically in the pancreatic .beta. (justia.com)
Drosophila1
The trans -acting factors are also conserved between Drosophila and human and have important functions, not only in development but also in stem-cell maintenance and cancer. (nih.gov)
Cell3
and allowing bacterial endotoxin (a bacterial cell wall component and potent activator of the immune system) to leak into your body. (mindbodygreen.com)
The use of cell lines does, however, introduce a risk the immunoglobulin (Ig)-secreting plasma cell in the spleen, of obtaining cell line-specific features as a result of the trans- gut, or BM [1, 2]. (lu.se)
These results show that LMP1 , mour viruses classified by IARC in EBV-specific T-cell response. (who.int)
System1
The HIV-Trans-Activator of Transcription (Tat) is known to persist in the central nervous system (CNS) of chronically infected HIV+ individuals. (uab.edu)
Activity2
In its paper published in Nature Biotechnology , the team demonstrated that designed hp-sgRNAs can tune the activity of a transactivator based on Cas9 from Streptococcus pyogenes (SpCas9). (genomeweb.com)
Based on the crystal structure of LbCas12a/crRNA/dsDNA (PDB ID: 5XUS) 12 , we reasoned that crRNA extensions can influence the trans-cleavage activity by either activating or inhibiting the catalytic efficiency of Cas12a, allowing us to better understand crRNA design with tunable trans-cleavage activity. (biorxiv.org)
Target1
At the off-target sites, hp-sgRNAs showed decreases of 91 percent, 79 percent, and 67 percent [for SpCas9, SaCas9 and AsCas12a, respectively] relative to WT-sgRNAs, compared with decreases of 88 percent, 38 percent, and 0 percent for the ns-sgRNAs," the researchers wrote. (genomeweb.com)
Studies5
Recent studies based on the National Institutes of Health-sponsored National Emphysema Treatment Trial have shown that the progression of COPD is associated with thickening of the walls of the small conducting airways by a repair and remodelling process 2 . (ersjournals.com)
Case-control studies have shown that the E1A DNA from group C adenoviruses is present in greater amounts in the lungs of patients with COPD than in controls with normal lung function matched for age, sex and smoking history 5 , and that the excess inflammation in the lungs of smokers with severe emphysema is associated with increased numbers of alveolar epithelial cells expressing E1A 6 . (ersjournals.com)
Studies have shown that estrogen actually delays gastric emptying 1 , which can lead to constipation. (mindbodygreen.com)
Studies show that a daily dose of these beneficial bacteria can improve constipation by improving consistency, transit time, and stool caliber. (mindbodygreen.com)
In several preclinical studies, HIV-Tat has been shown to be neurotoxic and important for HIV pathogenesis. (uab.edu)
Domains1
Among the established custom DNA binding domains, Cas9 is most easily scaled to facilitate genome-scale perturbations 3 , 4 due to its simplicity of programming relative to zinc finger proteins and transcription activator-like effectors (TALEs). (cdc.gov)
Study2
The purpose of this study was to evaluate the influence of the vertical and horizontal dimensions of the construction bite in the dentoskeletal changes induced by Klammt Open Elastic Activator during the treatment of Class II division 1. (bvsalud.org)
This study investigated the effect of the trans-resveratrol supplementation on the cardiac oxidative stress in rats exposed to cigarette smoke. (bvsalud.org)
Connection1
This is a "connection" page, showing publications Daynna Wolff has written about Trans-Activators. (musc.edu)
Function1
Activator appliances change the function and the position of the mandible in order to transmit forces to the dentition and the basal bone1. (bvsalud.org)
Great2
All samples showed great true positive, true negative, and false discovery rates considering the database size containing all modified and unmodified nucleosides referenced in the literature. (cnrs.fr)
Trans-resveratrol is a polyphenol with great antioxidant action that reduces the oxidative stress. (bvsalud.org)
Months1
Animals were exposed to cigarette smoke and supplemented with trans-resveratrol (6.0 mg kg-1) for two months. (bvsalud.org)
Effects1
11 Though these modifications were employed to utilize the cis-cleavage aspect of the CRISPR/Cas systems, the effects of such alterations on the trans-cleavage remain unknown. (biorxiv.org)