• Each Rapid HPLC DNA Oligo is quality controlled by electrospray ionization-mass spectrometry and capillary electrophoresis or analytical HPLC. (idtdna.com)
  • Purified oligos up to 60 bases that are provided with a purity guarantee receive QC by capillary electrophoresis (CE). (idtdna.com)
  • Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) is a method of separating molecules based on the difference of their molecular weight. (wikipedia.org)
  • After resolution of the proteins by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and fluorography, the radioactivity was associated with a major protein band with an apparent molecular mass of 60 kDa that was present in both microsomes and plasma membranes. (aspetjournals.org)
  • Proteolysis was not the main reason for differences in WBSF values between ES and NES muscles as judged by qualitative sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). (eurekamag.com)
  • By comparing the relative ratio of the distance traveled by each protein to the length of the gel (Rf) one can make conclusions about the relative molecular weight of the proteins, where the length of the gel is determined by the distance traveled by a small molecule like a tracking dye. (wikipedia.org)
  • Rotaviruses are segmented, double-stranded (ds) RNA G12 strains were further examined by polyacrylamide gel viruses that possess a triple-layered protein capsid. (cdc.gov)
  • Because a limited amount of EPO products was available, we were restricted to the common assays used for quality control: high performance size exclusion chromatography (HP-SEC) to assess monomer and aggregate content, ELISA to determine EPO protein content, SDS-PAGE under non-reducing conditions to detect possible fragmentation and covalent protein aggregates, capillary zone electrophoresis (CZE) for isoform profiling and a normocythemic mouse assay to test for potency. (springer.com)
  • Students then use denaturing and nondenaturing polyacrylamide gel electrophoresis to characterize the protein composition of their protein shakes. (bioone.org)
  • Short term cultured rat hepatocytes were used to further study the formation and nature of protein adducts after exposure to diclofenac. (aspetjournals.org)
  • Protein identification in Polyacrylamide Gel Electrophoresis (PAGE) requires post-electrophoretic steps like fixing, staining and destaining of the gel, which are time consuming and cumbersome. (iisertvm.ac.in)
  • Samples with equal amounts of protein (50 μg) were separated on 15% SDS-polyacrylamide gel electrophoresis transferred onto nitrocellulose membranes and probed with RhoA antisera (Cytoskeleton). (bio2009.org)
  • Polyacrylamide gel electrophoresis (PAGE) is a technique widely used in biochemistry, forensic chemistry, genetics, molecular biology and biotechnology to separate biological macromolecules, usually proteins or nucleic acids, according to their electrophoretic mobility. (wikipedia.org)
  • At the pH at which gel electrophoresis is carried out the SDS molecules are negatively charged and bind to proteins in a set ratio, approximately one molecule of SDS for every 2 amino acids. (wikipedia.org)
  • In most proteins, the binding of SDS to the polypeptide chains impart an even distribution of charge per unit mass, thereby resulting in a fractionation by approximate size during electrophoresis. (wikipedia.org)
  • Among rotavirus antigen-positive specimens, nization_monitoring/burden/rotavirus_estimates/en/index. (cdc.gov)
  • The electrophoresis (PAGE) of rotavirus dsRNA followed by 11 dsRNA segments, upon separation by electrophoresis, silver staining to determine the rotavirus electropherotype exhibit profi les that can be broadly categorized into long as previously described ( 10 ). (cdc.gov)
  • More recently, a novel inter-genogroup, G3P[8] reassortant variant with a short electropherotype, has emerged in Asia, Oceania and Europe, denoting an overall potential of unusual rotavirus strains. (scite.ai)
  • Native-PAGE keeps the oligomeric form intact and will show a band on the gel that is representative of the level of activity. (wikipedia.org)
  • Polyacrylamide gel electrophoresis (PAGE) separates full-length product from shorter species based on electric charge. (idtdna.com)
  • Two original products, Eprex (epoetin alfa) and Dynepo (epoetin delta), and two biosimilar products, Binocrit (epoetin alfa) and Retacrit (epoetin zeta), were compared using (1) high performance size exclusion chromatography, (2) ELISA, (3) SDS-PAGE, (4) capillary zone electrophoresis and (5) in-vivo potency. (springer.com)
  • subgroup ELISA to determine the VP6 subgroups and polyacrylamide gel electrophoresis (PAGE) to determine the electropherotypes present. (bvsalud.org)
  • Para a caracterização molecular dos genótipos G e P foram realizadas as reações de RT-PCR e a análise dos eletroferotipos por eletroforese em gel de poliacrilamida (PAGE). (rsbmt.org.br)
  • During a RVA surveillance in Brazil, G3P[8] strains were found displaying a short electropherotype pattern, which had not been detected before in this region. (scite.ai)
  • Thus, we found that characteristic equine‐like G3P[8] strains with a short RNA electropherotype are becoming highly prevalent in children and adults in Thailand. (scite.ai)
  • Results: The long electropherotypes (47.05) of four variations dominated over the short electropherotype (17.64). (bvsalud.org)
  • The short electropherotype was exhibited only by G2 genotype strains. (rsbmt.org.br)
  • Methods: Qligodeoxyribonucleotides (ODNs) were prepared with 111 In attached by diethylenetriaminepentaacetic acid (DTPA) at the 5' end or 3' end through a long chemical linker or to an internal nucleotide position through a short linker. (korea.ac.kr)
  • It is noteworthy that the equine-like G3P [8] strains with a short RNA profile that had been recently detected in several countries were predominant (65.3% to 89.8%) in various age groups across Thailand since 2015. (scite.ai)
  • 96.4%) of the G3P[8] strains exhibited a short RNA profile. (scite.ai)
  • Successful determination of the nucleotide sequences of the VP7 genes of 98 G3P[8] strains with a short RNA profile showed that they are all equine‐like G3P[8] strains. (scite.ai)
  • Enzymes hydrolyzing water-soluble short-chain triacylglycerols to corresponding fatty acids and glycerol are termed esterases (EC 3.1.1.1), and enzymes catalyzing the hydrolysis of carboxylic ester bonds at the lipid-water interface are termed lipases (EC 3.1.1.3). (iisertvm.ac.in)
  • Activity staining was done to prove that the biochemical activity of the enzymes was preserved after electrophoresis. (iisertvm.ac.in)
  • Short-chain dehydrogenases/reductases form a large, evolutionarily old family of NAD(P)(H)-dependent enzymes with over 60 genes found in the human genome. (ox.ac.uk)
  • This extends the previously recognized catalytic triad of Ser-Tyr-Lys residues to form a tetrad of Asn-Ser-Tyr-Lys in the majority of characterized short-chain dehydrogenases/reductase enzymes. (ox.ac.uk)
  • Molecular characterization of the G and P genotypes was performed using RT-PCR and electropherotyping analysis by polyacrylamide gel electrophoresis. (rsbmt.org.br)
  • The supplied adaptors are short synthetic oligonucleotide pre-annealed duplexes with 5' blunt end. (genelink.com)
  • Subsequent formation of DNA duplexes and triplexes was confirmed by gel electrophoresis. (korea.ac.kr)
  • Y. S. Zhu , Wu, H. , and Lai, J. H. , " Structure and polymorphism of novel X-chromosome short tandem repeat loci in a Chinese Han population " , vol. 14, pp. 15044-15049, 2015. (geneticsmr.com)
  • Recently, 5 novel X-chromosome short tandem repeat (X-STR) loci with high degrees of polymorphism were examined. (geneticsmr.com)
  • The 5 X-STR loci were successfully examined by polyacrylamide gel electrophoresis in a total of 200 unrelated Shaanxi Han individuals (100 males and 100 females). (geneticsmr.com)
  • The 111 In-induced breaks were assayed in denaturing polyacrylamide gel electrophoresis with a single-nucleotide resolution. (korea.ac.kr)
  • and short RNA patterns, termed electropherotypes. (cdc.gov)
  • Linkers are short synthetic palindromic sequences that self anneal to form blunt ended double stranded fragments. (genelink.com)
  • Polyacrylamide gel electrophoresis is a powerful tool used to analyze RNA samples. (wikipedia.org)
  • Samples were collected after written consent was giv- nization Program and, after March 2006, became available en by the parents. (cdc.gov)
  • Mass spectrometric analysis and deletion mutagenesis indicated that nsp10a may be short of about 70 amino acids in the N terminus of nsp10. (bvsalud.org)
  • 5 ]. Electrophoresis on polyacrylamide gel sions of 2 individuals who had been was carried out according to the methods immunized during the war. (who.int)
  • It is useful to make polyacrylamide gel via acrylamide hydration because pore size can be regulated. (wikipedia.org)
  • Due to the short size the Tm is low and thus even room temperature storage should be avoided. (genelink.com)
  • The greatest of the Greek warriors at Troy, Achilles deliberately chose a short life and a hero's death over a long life of average distinction. (learner.org)
  • For foreign short- or long-term guests the focus on colloid science in general, and the effort to keep an open attitude, provides a basis for a scientifically fruitful exchange. (lu.se)
  • Polyacrylamide gel with small pores helps to examine smaller molecules better since the small molecules can enter the pores and travel through the gel while large molecules get trapped at the pore openings. (wikipedia.org)
  • Linkers are short oligos that are supplied in an annealed form. (genelink.com)
  • As with all forms of gel electrophoresis, molecules may be run in their native state, preserving the molecules' higher-order structure. (wikipedia.org)
  • Critical residues for structure and catalysis in short-chain dehydrogenases/reductases. (ox.ac.uk)