• 5 Two methods of quick and simple plating of single HeLa cells that results in the growth and formation of colonies from individual cells were detailed in a paper that was published in 1956. (onclive.com)
  • While yeast mutants lacking β subunit have stable aggregated F(1) α subunit in the mitochondrial matrix, the human α subunit is completely degraded in cells deficient in F(1) β subunit. (staffs.ac.uk)
  • In cellular biology, stress granules are biomolecular condensates in the cytosol composed of proteins and RNAs that assemble into 0.1-2 μm membraneless organelles when the cell is under stress. (wikipedia.org)
  • The stress proteins that are the main component of stress granules in plant cells are molecular chaperones that sequester, protect, and possibly repair proteins that unfold during heat and other types of stress. (wikipedia.org)
  • The reactive oxygen species (ROS), glutathione (GSH), carbonyl content in proteins (CCP), DNA-protein crosslinks (DPC) and cell viability were measured. (scirp.org)
  • The Thermo Scientific 1-Step Human High-Yield In Vitro Translation (IVT) Kits enable the expression of functional proteins with 10 to 100 times greater yield per mL than other mammalian IVT systems. (thermofisher.com)
  • The 1-Step High-Yield IVT System uses modified HeLa cell extracts to take advantage of the robust human translation machinery and generate functional full-length proteins. (thermofisher.com)
  • The advantages of using the 1-Step Human High-Yield IVT Kits over traditional in vivo expression systems include the ability to express toxic or insoluble proteins, easily perform protein labeling with modified amino-acids and reduce the time and cost of expressing human proteins in tissue culture cells. (thermofisher.com)
  • Autophagy is generally considered as a cell survival/protection mechanism because it removes toxic or obsolete proteins and organelles and recycles the degradation products for use as sources for energy and metabolites in anabolic pathways 3 . (nature.com)
  • To investigate CBC's role in splicing, we used mass spectrometry to identify proteins that copurify with mammalian CBC. (iucc.ac.il)
  • Mammalian cells are mostly used in the biopharmaceutical industry for the production of recombinant therapeutic proteins. (medgadget.com)
  • 3,4 There is, however, one pressing issue with the discovery that has negatively impacted the relationship between the medical community and with patients of color: Lacks' cervical cancer cells were collected without her or her family's consent. (onclive.com)
  • Experience with the culture and transfection of mammalian cell lines such as HEK293, CHO, HeLa, Vero cells. (peccoud.org)
  • Based on these findings, investigators concluded that these methods would be useful for examining mammalian cell growth, physiology, genetics, and biochemistry in addition to examining how cells respond to drugs, viruses, and high-energy radiation. (onclive.com)
  • However, emerging evidence suggests that autophagy is a primary mechanism of cell death (autophagic cell death, ACD) and implicates ACD in several aspects of mammalian physiology, including tumor suppression and psychological disorders. (nature.com)
  • 4 ] in 1951 cell culturing has become one of the most widely used methods with exceptional contribution to the advances in almost all fields of contemporary biology - cell biology, genetics, cell biochemistry, physiology etc. (intechopen.com)
  • Studying single-cell physiology and responses to environmental triggers is crucial for increased understanding of how microbes in soil function and contribute to larger scale biogeochemical cycles. (lu.se)
  • In an effort to further understanding of the effects of radiofrequency (RF) radiation on somatic cell mitotic activity and germ cell function, cell suspensions were exposed to 27 megahertz (MHz) or 2,450MHz radiation under isothermal conditions in-vitro. (cdc.gov)
  • RNA interference-mediated depletion of HSPC117 inhibited maturation of intron-containing pre-tRNA both in vitro and in living cells. (nih.gov)
  • A defined synthetic mixture of amino acids, salts, carbohydrates, vitamins and serum was shown to support cells in vitro[ 3 ], thus unifying a major variable in cell culturing experiments and providing a possibility for rapid development of this novel method. (intechopen.com)
  • The bacteria-produced GM-CSF stimulated the maturation of bone marrow-derived dendritic cells (BMDCs), while IL-7 promoted the proliferation of spleen isolated T cells and inhibited cytotoxicity T cell apoptosis in vitro. (bvsalud.org)
  • These inhibitors inhibit NHEJ-catalyzed DSB repair and sensitize in vitro cancer cells to DSB-inducing agents. (termedia.pl)
  • BRSK2 expressed in insect cells specifically phosphorylates WEE1A, CDC25C and CDC25B in an in vitro assay. (signalchem.com)
  • Because NS is often observed during the mammalian CA test (in vitro), we examined the relationship between NS and CAs. (biomedcentral.com)
  • Non-Mammalian Cells (Bacteria, etc. (metabolon.com)
  • The first method utilizes irradiated, non-multiplying feeder cells to condition the medium, while the second method utilizes a similar, but gentler, strategy that involves plating bacteria on semisolid nutrient media. (onclive.com)
  • The bacteria experienced restricted levels of iron, magnesium, and phosphate in both host cell types, as shown by up-regulation of the sitABCD system, the mgtA gene, and genes of the phoBR regulon. (strath.ac.uk)
  • Moreover, the tumor-toxic GZMB+ CD8+ T cell and IFN-γ+ CD8+ T cell populations conspicuously increased with the treatment of engineered bacteria. (bvsalud.org)
  • We captured early stage interactions of invasive-bacteria and HeLa cells during the process of bacteria-mediated gene delivery and illustrate sufficient clarity can be obtained using this procedure to preserve and clearly visualize relevant cellular structures. (um.edu.my)
  • Using activity-guided purification of tRNA ligase from HeLa cell extracts, we identified HSPC117, a member of the UPF0027 (RtcB) family, as the essential subunit of a tRNA ligase complex. (nih.gov)
  • In the present study we investigated the efficacy of Organic extracts of Nigella sativa seed powder for its clonogenic inhibition and induction of apoptosis in HeLa cancer cell. (biomedcentral.com)
  • Methanolic, n-Hexane and chloroform extracts of Nigella sativa seedz effectively killed HeLa cells. (biomedcentral.com)
  • All three extracts induced apoptosis in HeLa cells. (biomedcentral.com)
  • Cell types included resting human peripheral lymphocytes, LN71 or RT2 glioma cells, Chinese-hamster-ovary cells, HeLa cells, and mouse spermatozoa. (cdc.gov)
  • 7] "Functional characterization of an organic cation transporter (hOCT1) in a transiently transfected human cell line (HeLa). (tcdb.org)
  • Lipofection was used to transiently transfect the hOCT1 plasmid DNA in a human cell line, HeLa. (drugbank.com)
  • Transiently transfected HeLa cells represent a useful tool in studying the interactions and kinetics of organic cations and other xenobiotics with hOCT1 and in understanding the molecular events involved in organic cation transport. (drugbank.com)
  • The RNP is small (approximately 7S) and contains most of the nonribosome-associated 5S RNA molecules in HeLa cells. (nih.gov)
  • Splicing of mammalian precursor transfer RNA (tRNA) molecules involves two enzymatic steps. (nih.gov)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • Viruses to be sequenced were amplifi ed in Vero cells, TAR, Inc., Madison, WI, USA) and translated into deduced and viral RNA was extracted from cell culture supernatant amino acid sequence. (cdc.gov)
  • Virus-mediated gene therapy has the potential to deliver exogenous genetic material into specific cell types to promote survival and counteract disease. (frontiersin.org)
  • Administration of gene therapy viruses into skeletal muscle, where distal terminals of motor and sensory neurons reside, has been shown to result in extensive transduction of cells within the spinal cord, brainstem, and sensory ganglia. (frontiersin.org)
  • Gene therapy viruses are non-replicating, but still hijack host cell machinery to express transgenes of interest in the nucleus. (frontiersin.org)
  • Deletion of Jarid2 leads to impaired orchestration of gene expression during cell lineage commitment. (sdbonline.org)
  • 6] "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (tcdb.org)
  • We have determined the complete gene expression profiles for S. flexneri infecting human epithelial HeLa cells and human macrophage-like U937 cells. (strath.ac.uk)
  • Comparison with the gene expression of intracellular Salmonella serovar Typhimurium, which resides within the Salmonella-containing vacuole, indicated that S. flexneri is exposed to oxidative stress in U937 cells. (strath.ac.uk)
  • the chorion gene loci in follicle cells (21 22 In pre-RC development the effective launching of multiple MCM complexes is necessary for the toleration of replication strains and activation of checkpoint pathways (23-25). (exposed-skin-care.net)
  • The cdc25 gene product is a tyrosine phosphatase that acts as an initiator of M-phase in eukaryotic cell cycles by activating p34 cdc2 . (biologists.com)
  • Cas9-expressing HeLa cells can be transduced or electroporated with sgRNA targeting a gene of interest to quickly generate knock-out cell pools or cell lines. (genprice.com)
  • Herein, we engineered attenuated Salmonella typhimurium VNP20009 with gene circuits to synthetize granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin 7 (IL-7) within tumors, which recruited dendritic cells (DCs) and enhanced T cell priming to elicit anti-tumor response. (bvsalud.org)
  • p53, a tumor suppressor gene, helps regulate the cell cycle. (biomedcentral.com)
  • Checkpoint kinase 2 (CHEK2), a cell cycle checkpoint regulator gene, codes for a kinase protein activated in response to radiation and other agents that cause breaks in the DNA. (termedia.pl)
  • Cell lines are used in a variety of research applications, including drug discovery and development, gene therapy, and tissue engineering. (medgadget.com)
  • L5178Y is often used in mouse lymphoma L5178Y cell Tk (thymidine kinase) gene mutation assays. (biomedcentral.com)
  • Effects of 27 MHz radiation on somatic and germ cells. (cdc.gov)
  • Genetics of somatic mammalian cells. (wikidata.org)
  • This theme seems to be common in bacterial infection, because the Ipa-Mxi-Spa-like type III secretion systems were also down-regulated during mammalian cell infection by Salmonella enterica serovar Typhimurium and Escherichia coli O157. (strath.ac.uk)
  • Therefore, in this review, we avoid a lengthy repetition of the description of autophagy and cell death processes and focus on the death-promoting roles of autophagy and the intertwined connection between autophagy and apoptosis. (nature.com)
  • Our results might reveal the system of apoptosis, induced with the TRBP-bound miRNAs through the connections of TRBP with LGP2, as an antiviral immune system in mammalian cells. (innovation-ecosystems-agora.com)
  • Our finding demonstrated which the crosstalk between RNA silencing and RLR signaling features to stimulate apoptosis as an antiviral immune system in mammalian cells. (innovation-ecosystems-agora.com)
  • Apoptosis an active physiological process resulting in cellular self-destruction of unwanted cells is absent in cancer cells. (biomedcentral.com)
  • It is also involved in cell cycle arrest and apoptosis [17]. (termedia.pl)
  • Glucose withdrawal induces cell death that can occur by apoptosis (in Bax, Bak-deficient mouse embryonic fibroblasts or HeLa cells) or by necrosis (in Rh4 rhabdomyosarcoma cells). (gla.ac.uk)
  • Inhibition of autophagy by chemical or genetic means by using 3-methyladenine, chloroquine, a dominant negative form of ATG4B or silencing Beclin-1, Atg7, or p62 indicated that macroautophagy does not protect cells undergoing necrosis or apoptosis upon glucose deprivation. (gla.ac.uk)
  • The outcomes indicated that publicity to Cu-NPs led to vital decreases within the relative uterine weight coefficients and will increase in inflammatory cell infiltration, mitochondrial swelling and vacuolization, shortened and lowered endometrial epithelial cell microvilli, and apoptosis. (caspase-14.com)
  • Glutamine mediates protein turnover via cellular mTOR (mammalian target of rapamycin) signalling. (sigmaaldrich.com)
  • They are assembled to segregate chromosomes to the forming daughter cells (Movie 1). (tu-dresden.de)
  • Among these is the isolation of the first line of murine stem cells [ 5 , 6 ] in 1981, followed by establishment of the first human embryonic stem cell lines by Thompson [ 7 ]. (intechopen.com)
  • DUGV is transmitted by further characterization of this virus, including growth kinet- ics in cell culture and full-length genome sequencing and ticks to vertebrates, including humans, and causes a mild genetic characterization, which show it to be distinct from febrile illness and thrombocytopenia ( 2 ). (cdc.gov)
  • The purpose of the present study was to develop a mammalian expression system for hOCT1 and to characterize the interactions of various compounds with the cloned transporter. (drugbank.com)
  • We tested the interaction of an array of organic cations and other compounds with hOCT1 by determining Ki values in inhibiting 14C-tetraethylammonium (TEA) transport in the transfected cells. (drugbank.com)
  • 14C-TEA uptake in hOCT1 plasmid DNA-transfected HeLa cells was trans-stimulated by unlabeled TEA and 1-methyl-4-phenyl-pyridinium. (drugbank.com)
  • Current knowledge of cellular behavior is mainly acquired by studies concerning homogenous populations of cells cultured as monolayers. (intechopen.com)
  • This is due to acute alterations in the tissues surrounding the tumor and in the environment as a result of treatment with anticancer therapeutics, which facilitate the drug resistance of tumor cells. (oncotarget.com)
  • Both the structure of the PGs and the GAG chains, and the expression of the enzymes involved in their biosynthesis and degradation, vary between normal cells and tumor cells. (lu.se)
  • Chromatin immunoprecipitation and live-cell imaging assays revealed decreased cotranscriptional accumulation of U4/U6·U5 snRNPs on active transcription units, consistent with a requirement for CBC in cotranscriptional spliceosome assembly. (iucc.ac.il)
  • The results indicated that porous spherical CaCO 3 microparticles may induce oxidative damage to Hela cells. (scirp.org)
  • NS measurements were carried out using compounds known to induce structural CAs in the mouse lymphoma L5178Y cell line. (biomedcentral.com)
  • 2017). My intention is to apply this approach now for a quantitative analysis of mitotic spindle structure in mammalian cells. (tu-dresden.de)
  • Overexpression of BRSK2 induces G2/M arrest in HeLa cells while small interfering RNA against BRSK2 partly abrogated UV-induced G2/M arrest. (signalchem.com)
  • This competitive binding inhibits the binding of 40 types of pre-miRNAs with TRBP in HeLa cells, aswell as the maturation of these miRNAs by Dicer/TRBP, and their following RNA silencing actions (15). (innovation-ecosystems-agora.com)
  • This double stranded break is repaired in mammalian cells either through Non-Homologous End Joining or Homologous Recombination. (genprice.com)
  • AT001] Basal Medium developed by Harry Eagle is a combination of essential nutrients in appropriate concentrations for monolayer cultivation of a wide variety of normal and transformed cells. (ols-bio.de)
  • This cell line, dubbed the HeLa cell line after Lacks' name, has since become one of the most widely used human cell lines in biologic research over the past half century, with the cell lines having been used in over 100,000 scientific publications on PubMed alone. (onclive.com)
  • Then, a survey is given about ATP1A2 mutations implicated in migraine cases as documented in the literature with focus on mutations that were described to completely destroy enzyme function, or lead to misfolded or mistargeted protein in particular model cell lines. (frontiersin.org)
  • Cell banks allow the storage and generation of detailed characterizations of cell lines with specific genomes for research use. (medgadget.com)
  • Growing importance of cell lines in research: Cell lines are widely used in basic and applied research, and their importance is expected to continue to grow in the coming years. (medgadget.com)
  • Rising demand for cell lines for producing vaccines to combat the COVID-19 outbreak is boding well for the growth of the mammalian cell banking market. (medgadget.com)
  • Shigella flexneri, the etiologic agent of bacillary dysentery, invades epithelial cells as well as macrophages and dendritic cells and escapes into the cytosol soon after invasion. (strath.ac.uk)
  • Although the research that emerged from the discovery of the HeLa cell line has helped to prevent 4.5 billion global infections and 10.3 million global deaths, the unethical and controversial nature of their discovery has raised issues with regard to privacy and consent in underrepresented patient populations. (onclive.com)
  • The discovery of the immortal, ever-replicating HeLa cell line in 1952 has led to several breakthroughs in biomedical research for countless diseases including cancer, AIDs, and polio. (onclive.com)
  • In honor of Black History Month, Markman looks back on the progress that has been made through the HeLa cell line, incidents that have contributed to a lack of trust in the medical community, and efforts that need to be made to ensure that the rights of underserved patient populations are maintained in clinical cancer trials. (onclive.com)
  • Since the establishment of the first cell line by Gey et al. (intechopen.com)
  • Human wild-type HeLa cell line ( ab255928 ). (abcam.com)
  • Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1. (abcam.com)
  • For example, in June 2019 , Selexis SA announced a partnership with Avacta Group Plc for cell line development. (medgadget.com)
  • In a short-term CA test using the fibroblast CHL cell line, the appearance of NS increased in a dose-dependent manner after exposure to six types of clastogens (mitomycin C, methyl methane sulfonate, 1-methyl-3-nitro-1-nitrosoguanidine, benzo[a]pyrene, cyclophosphamide monohydrate, and 9,10-dimethyl-2-benzanthracene), and a strong correlation was found between NS (%) and CAs (%) at each dosage. (biomedcentral.com)
  • Therefore, we hypothesized that clastogens cause NS in cultured mammalian cells, since the mouse lymphoma L5178Y cell line is known to have a similar sensitivity to clastogens. (biomedcentral.com)
  • In this study, the correlation between NS and CAs was examined using CA tests for clastogens in the Chinese hamster lung CHL/IU cell line. (biomedcentral.com)
  • The CHL cell line was obtained from the Health Science Research Resources Bank (HSRRB, Osaka, Japan). (biomedcentral.com)
  • This review focuses on an emerging topic, the functional involvement of AQPs in ROS membrane transport, with specific regard to the movement of hydrogen peroxide and NO into and out of cells, in both health and oxidative stress-induced diseases. (hindawi.com)
  • Nevertheless, the definition of autophagic cell death (ACD) has been neither universally understood nor unanimously accepted in the field 4 . (nature.com)
  • Half a century ago, Christian de Duve coined the term "autophagy" (literally, "self-eating" in Greek) to describe a process where the cell digests its cytoplasmic materials within lysosomes 1 . (nature.com)
  • Therefore, the relationship between autophagy and cell death remains unclear and warrants further study to harness autophagy for the treatment of various human diseases. (nature.com)
  • Starvation of glucose, an essential nutrient, is thought to promote autophagy in mammalian cells. (gla.ac.uk)
  • We thus aimed to determine the role of autophagy in cell death induced by glucose deprivation. (gla.ac.uk)
  • Because efficient loading of excess MCM complexes is usually thought to be required for cells to tolerate replication tension Cdt1- and SNF2H-mediated advertising of MCM loading could be biologically relevant for the legislation of DNA replication. (exposed-skin-care.net)
  • One of the older innovations to emerge from the discovery were methods for testing for cancerous cells that proved to be so reliable, they continue to be used by investigators in the modern age. (onclive.com)
  • L-glutamine is an essential amino acid that is a crucial component of culture media, and serves as a major energy source for cells in culture. (sigmaaldrich.com)
  • Qualified for use in cell culture applications, especially serum-free cell culture. (sigmaaldrich.com)
  • Components AND Strategies Cell culture Individual HeLa wild-type (WT), LGP2? (innovation-ecosystems-agora.com)
  • Hazara virus (HAZV) and Dugbe virus (DUGV), and par- man adrenal cortex carcinoma), HeLa (human cervical tial sequences are available for many other members of the adenocarcinoma), HUH-7 (human hepatocarcinoma), and C6/36 ( Aedes albopictus mosquito) cells in culture. (cdc.gov)
  • EXPERIMENTAL PROCEDURES Cell Culture and Synchronization HEK293T T98G and HeLa cells were produced in Dulbecco's altered Eagle's medium with 8% fetal calf serum. (exposed-skin-care.net)
  • 2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. (abcam.com)
  • 3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method. (abcam.com)
  • Based on cell count, seed cells in an appropriate cell culture flask at a density of 2x10 4 cells/cm 2 . (abcam.com)
  • Additionally, with the research community's ever-evolving needs, the demand for specialized cell culture has grown, and due to this, most manufacturers that are currently offering these services cannot depend solely on sales from catalog services. (medgadget.com)
  • Deletion of ATF4 in parental cancer cells significantly reduces colony formation and metastasis of NDCs, whereas overexpression of ATF4 activates the nonclassical NF-κB signaling pathway to promote chemotherapy-induced metastasis of NDCs. (bvsalud.org)
  • In addition, TSA-mediated cell death was reduced by the overexpression of HIF-1α but it was rescued by transfection with a HIF-1α mutant (K674R). (oncotarget.com)
  • However, patients suffered with tumors that had inadequate infiltrated immune cells only receive limited therapeutic benefits from ICB therapy. (bvsalud.org)
  • For cell cycle synchronization T98G cells were rendered quiescent by serum starvation for 48 h and then released into the cell cycle by serum stimulation. (exposed-skin-care.net)
  • Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose. (abcam.com)
  • The CHL cells were cultured in Eagle's MEM supplemented with 10 % (v/v) heat-inactivated calf serum. (biomedcentral.com)
  • The L5178Y cells were cultured in RPMI1640 supplemented with 10 % (v/v) heat-inactivated horse serum and sodium pyruvate (200 μg/ml) in a humidified incubator at 37 °C with 5 % CO 2 . (biomedcentral.com)
  • With increasing use of technology, rapid industrialization, and more research programs, growing economies such East Asia will experience increasing growth in the mammalian cell banking market. (medgadget.com)
  • 14] "The expression of the solute carriers NTCP and OCT-1 is regulated by cholesterol in HepG2 cells. (tcdb.org)
  • Throughout the years, ROS and NO have been widely considered to enter cells by freely diffusing through the cell membrane lipid bilayer and not via specific transporters or channels. (hindawi.com)
  • Instead of being translationally down-regulated, HeLa cells lacking F(1) degrade Atp6p, thereby preventing proton leakage across the inner membrane. (staffs.ac.uk)
  • For successful transgene expression, viruses administered into muscle must undergo a series of processes, including host cell interaction and internalization, intracellular sorting, long-range retrograde axonal transport, endosomal liberation, and nuclear import. (frontiersin.org)
  • Programmed cell death (PCD), as described by Lockshin and Williams 14 , is defined as controlled cell death evoked by intracellular systems. (nature.com)
  • Studies were also conducted on liposome permeability, and a mathematical model was used to calculate radiation energy absorption in mammalian cell models. (cdc.gov)
  • Numerous studies have evaluated nuclear swelling (NS), including in cultured mammalian cells (e.g. (biomedcentral.com)
  • The efficient DNeasy Blood & Tissue procedure enables high yields of total DNA from animal blood and tissue samples (see table Typical DNA yields from animal tissues using DNeasy Blood & Tissue Kits and figure DNA yields. DNA was purified from 100 µl whole blood using the DNeasy Blood & Tissue Kit. Goat : DNA was purified from 50 µl goat whole blood. Using more than 50 µl goat blood gave no significant increase in DNA yield. Chicken : DNA was purified from 5 µl chicken whole blood. Bird blood contains nucleated erythrocytes, giving higher DNA yields than mammalian blood. "> DNA yields ). (qiagen.com)
  • A central aspect is then the ability to optimize the functional properties with high spatial resolution, creating materials that are able to control the interaction with the biological surrounding at the nanoscale thus guiding the responses of biomolecules, cells and tissues. (european-mrs.com)
  • Virtually, engineered VNP20009 prefer to colonize in tumors, and inhibited tumor growth by enhancing DCs and T cell infiltration. (bvsalud.org)
  • In addition, silencing of Foxg1 significantly reduced the concentration of testosterone and estrogen in cultured primary granulosa cells (GCs) and TCs (P (bvsalud.org)
  • 2015) . Jarid2 methylation via the PRC2 complex regulates H3K27me3 deposition during cell differentiation . (sdbonline.org)
  • Jarid2 methylation is shown to be important to promote PRC2 activity at a locus devoid of H3K27me3 and for the correct deposition of this mark during cell differentiation. (sdbonline.org)
  • Mammalian cells produce PGs, which are involved in biological processes such as cell growth and differentiation. (lu.se)
  • This continuous-exchange cell-free (CECF) system enables protein expression in an overnight incubation of up to 750 µg/mL. (thermofisher.com)
  • The 1-Step Human High-Yield IVT Kits are cell-free protein expression systems that provide all of the essential components required for transcription and translation. (thermofisher.com)
  • Plasmids Mammalian expression vectors pCLMSCVhyg-T7-Cdt1 pcDNA3.1-3HA-Cdt1 and pcDNA3.1-zeo-FLAG-ORC1 and bacterial expression vector pGEX-6P1-Cdt1 were described previously (5 12 The pEGFP-C1 expression vector was purchased from Clontech. (exposed-skin-care.net)
  • Here we show normal expression of Atp6p in HeLa cells depleted of the F(1) β subunit. (staffs.ac.uk)
  • The 2 cell types possess volumes, nuclear cross-sections, plating efficiencies, and generation times which are identical within experimental error, but display widely different cross-sectional areas, suggesting that the basic change occurs in the cell surface," wrote the authors of the study. (onclive.com)
  • we obtained additional information nearly simultaneously through additional microgram-scale sampling during the respective cell assays. (biomedcentral.com)
  • In this study, we assessed the biological safety of porous spherical CaCO 3 microparticles on Hela cells. (scirp.org)
  • Scientists working in basic, translational, and clinical cancer metabolism research are invited to join the Academy in New York on April 17th to discuss the intersection between cell signaling and metabolism. (nyas.org)
  • Cancer Cell Metabolism: Unique Features Inform New Therapeutic Opportunities. (nyas.org)
  • Advanced Cell Technology (ACT), a stem cell biotechnology company in Worcester, Massachusetts, showed the potential for cloning to contribute to conservation efforts. (asu.edu)