Cell-bound proteinsAntibodyAlbuminConcentrationsAntibodiesClinicalIntracellularTIBCLigandsImmuneComplementSignificantly higherCharacterizeDiagnosisPrimarilyDecreasesLevelsMoleculesPolypeptideParametersReceptorsDegradationConjugateHemoglobinMutationsDeficiencySevereResiduesAcidFoundVariationsStudyRatioBloodSamplesTotalHealthyComplexRangeActivityGrowthSpecificBodyCells
Cell-bound proteins2
- The complement system consists of more than 35 soluble and cell-bound proteins, 12 of which are directly involved in the complement pathways. (dadamo.com)
- However, most drugs act as haptens, binding covalently to serum or cell-bound proteins, including peptides embedded in major histocompatibility complex (MHC) molecules. (msdmanuals.com)
Antibody8
- Western blot analysis of extracts from Jurkat cells, untreated or calyculin A-treated (0.1 µM for 30 minutes), using Phospho-(Ser) 14-3-3 Binding Motif Antibody. (cellsignal.com)
- Phospho-(Ser) 14-3-3 Binding Motif Antibody ELISA Assay: Signal-to-noise ratio of phospho- versus nonphospho-14-3-3 binding motif peptides. (cellsignal.com)
- Western blot analysis of extracts from 293 cells transfected with Bad or Bad Ser112 and/or Ser136 mutant fusion proteins, using Phospho-(Ser) 14-3-3 Binding Motif Antibody (top), Phospho-Bad (Ser136) Antibody #9295 (middle) or Phospho-Bad (Ser112) Antibody #9291 (bottom). (cellsignal.com)
- Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing staining of proteins containing phosphorylated 14-3-3 binding motifs, using Phospho-(Ser) 14-3-3 Binding Motif Antibody. (cellsignal.com)
- Cortisol in standards and samples compete with cortisol conjugated to horseradish peroxidase for the antibody binding sites on a microtitre plate. (salimetrics.com)
- It is noteworthy that all of them share some escape mutations already known to have emerged under the selection pressure of antibody exposure, both from immune serum or therapeutic antibody use in prolonged survival within immunocompromised individuals, and from the antibodies elicited by natural infection. (news-medical.net)
- Some protein and large polypeptide drugs (eg, insulin , therapeutic antibodies) can directly stimulate antibody production. (msdmanuals.com)
- The binding makes the protein-drug complex immunogenic, stimulating antidrug antibody production, T-cell responses against the drug, or both. (msdmanuals.com)
Albumin5
- Human serum albumin (HSA) binding of anticancer plant alkaloid ellipticine has been studied in conjunction with assessing its association to serum a1-acid-glycoprotein (AAG). (jascoinc.com)
- C(H)-C 6 H 4 OH- o , o -phen = 1,10-phenanthroline, and OAc = CH 3 COO − ) with human serum albumin (HSA) was studied using fluorescence quenching, synchronous and 3D fluorescence measurements and UV-vis spectroscopy. (rsc.org)
- Diclofenac is more than 99% bound to human serum proteins, primarily to albumin. (nih.gov)
- Characterization and comparison of methylene diphenyl diisocyanate haptenated human serum albumin and hemoglobin. (cdc.gov)
- The objective of the present study was to characterize hemoglobin (Hb) and human serum albumin (HSA) following conjugation to different molar concentrations of MDI. (cdc.gov)
Concentrations3
- Self-polymerization of MDI onto protein was observed on some amino acids at higher MDI concentrations. (cdc.gov)
- GO patients displayed significantly higher LBP serum concentrations than healthy controls. (frontiersin.org)
- 4 g daily unless serum concentrations of total sulfapyridine and the phenotype of the patient are known. (drugs.com)
Antibodies3
- Graves' disease (GD), characterized by TSH-receptor stimulating antibodies and increased thyroid hormone serum levels, is an autoimmune disease affecting roughly 3% of the general population ( 1 ). (frontiersin.org)
- The researchers used two anti-RBD antibodies, mAb102 and mAb48, that bind to the RBDs in the 'up' conformation and thereby prevent RBD-ACE2 binding. (news-medical.net)
- When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. (bdbiosciences.com)
Clinical7
- This study aimed to investigate whether serum indicators related to iron stores in the body are associated with clinical and endoscopic disease severity. (nature.com)
- Underweight, decreased serum iron and TIBC were independent risk factors for moderate clinical disease. (nature.com)
- Decreases in serum iron and TIBC were associated with the clinical activity of CD. (nature.com)
- The GH study group of the Korean Endocrine Society aims to establish the Korean reference ranges of serum IGF-I and insulin-like growth factor binding protein-3 (IGFBP-3) and assess the relationship between IGF-I and IGFBP-3 and clinical parameters. (e-enm.org)
- Since 1986, when tests for measuring PSA levels in serum were introduced into clinical practice, early diagnosis and management of prostate cancer has been revolutionized, and much has been learned about the strengths and weaknesses of these assays. (medscape.com)
- the clinical significance of nonhapten TCR binding is being determined. (msdmanuals.com)
- Demographic and that this particular family of angiogenic clinical data were collected during routine proteins plays an important role in placental obstetric visits. (who.int)
Intracellular1
- 1994). High-res- belongs to the family of intracellular lipid bind- olution crystal structures (Sacchetti ni et al. (lu.se)
TIBC3
- Combined detection of decreased serum iron and TIBC was helpful in differentiating severe patients. (nature.com)
- The total iron-binding capacity (TIBC) test looks at how well the iron moves through your body. (rochester.edu)
- The TIBC test is based on certain proteins, including transferrin, found in the blood. (rochester.edu)
Ligands2
- Intestinal permeability was assessed by measuring serum lipopolysaccharide-binding protein (LBP), zonulin, TLR5, and TLR9 ligands. (frontiersin.org)
- Within the MicroGO cohort, patients with high serum LBP levels also showed higher levels of zonulin and TLR5 and TLR9 ligands in their circulation. (frontiersin.org)
Immune3
- It is derived from many small plasma proteins that form the biochemical cascade of the immune system. (dadamo.com)
- CR1 on erythrocytes may bind circulating immune complexes (that had activated complement) and transport them to the liver where the immune complexes are partially degraded and thus become more soluble. (dadamo.com)
- The increased intestinal permeability was accompanied by augmented expression of genes marking immune cell infiltration and encoding key proteins for immune cell adhesion, antigen presentation, and cytokine signaling in the orbital tissue. (frontiersin.org)
Complement1
- It binds as a cofactor to C3b/C4b inactivator (C3bina), which then hydrolyzes the complement fragment C4b. (lu.se)
Significantly higher1
Characterize2
- Taking advantage of the ability of chiral protein environments to induce optical activity, circular dichroism (CD) spectroscopy was employed to characterize the alkaloid-protein non-covalent adducts. (jascoinc.com)
- MDI haptenation of proteins is central to dNCO immunological sensitization, however, the resultant protein conjugates are complex and difficult to characterize. (cdc.gov)
Diagnosis2
- Measuring serum IGF-I and IGFBP-3 is essential for diagnosis and monitoring of treatment responses in GH deficiency such as hypopituitarism, growth hormone-releasing hormone (GHRH) receptor mutation, or GH insensitivity [ 8 ] and GH excess such as acromegaly or gigantism [ 9 ]. (e-enm.org)
- ABSTRACT We evaluated the prognostic value of serum endostatin and vascular endothelial growth factor (VEGF) for diagnosis of pre-eclampsia. (who.int)
Primarily1
- Molecular docking studies indicated that all three complexes primarily bind to HSA in subdomain IIA with amino acid residues such as Arg218 and Lys199 which are located at the entrance of Sudlow's site I. The in vitro cytotoxicities of complexes 1-3 against HeLa cells showed promising anticancer activity (IC 50 = 3.5, 3.9 and 16.9 μM for 1 , 2 and 3 , respectively). (rsc.org)
Decreases1
- cangrelor decreases effects of clopidogrel by receptor binding competition. (medscape.com)
Levels7
- 3D fluorescence studies showed that the HSA structure was altered at the secondary and tertiary levels upon binding with the complexes. (rsc.org)
- Higher levels of IGFBP-3 increase the binding capacity of IGF-I, but also decrease the circulating IGF-I bioavailability [ 2 ]. (e-enm.org)
- Studies consistently report high correlations between serum and salivary cortisol, indicating that salivary cortisol levels reliably estimate serum cortisol levels. (salimetrics.com)
- Moreover, serum LBP levels significantly correlated with the abundance of specific Gram-negative gut bacteria, linking the gut to local orbital inflammation. (frontiersin.org)
- Rising levels of PSA in serum are associated with prostate cancer. (medscape.com)
- We determined VEGF and endostatin levels in the sera of 20 healthy, non-pregnant women and 64 pregnant women: 20 healthy, 20 with mild pre-eclampsia and 24 with severe pre-eclampsia. (who.int)
- Serum levels of these factors in non-pregnant women were similar to those in healthy pregnant women. (who.int)
Molecules1
- Haptens may also bind directly to class II MHC molecules, directly activating T cells. (msdmanuals.com)
Polypeptide1
- It exists as an ~460 kDa protein formed from 18 polypeptide chains comprised of three different subunits named C1qa, C1qb, and C1qc. (bdbiosciences.com)
Parameters1
- Therefore, we hypothesized that the indicators related to serum iron parameters may be candidates for evaluating disease severity. (nature.com)
Receptors1
- Adherent receptors mediate adherence of cells and other particles with bound C3b or C4b fragments and are known as CR1 to CR5. (dadamo.com)
Degradation1
- eg, penicillin itself is not antigenic, but its main degradation product, benzylpenicilloic acid, can combine with tissue proteins to form benzylpenicilloyl (BPO), a major antigenic determinant. (msdmanuals.com)
Conjugate2
- Bound cortisol enzyme conjugate is measured by the reaction of the horseradish peroxidase enzyme to the substrate tetramethylbenzidine (TMB). (salimetrics.com)
- The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. (bdbiosciences.com)
Hemoglobin1
- found that serum hepcidin was positively correlated with IL-6 and negatively correlated with hemoglobin in anemia of chronic disease (ACD) 11 .This suggested that iron stored in these inflammatory cells is an important source of hematopoiesis in CD patients with ACD. (nature.com)
Mutations1
- These show combinations of mutations in the spike region, including the receptor-binding domain (RBD) and N-terminal domain (NTD), as well as other genomic regions. (news-medical.net)
Deficiency1
Severe2
- The three variants were incubated with serum from a range of convalescent COVID-19 subjects, who had mild, moderate and severe-to-critical disease. (news-medical.net)
- Symptoms range from mild to severe and include rash, anaphylaxis, and serum sickness. (msdmanuals.com)
Residues1
- More cross-linking was observed at 0.5 mg/ml HSA than at 5mg/ml at 40:1 MDI: HSA It is concluded that MDI has a greater reactivity toward HSA than Hb with respect to the number of residues haptenated and amount of MDI bound per mole of protein. (cdc.gov)
Acid2
Found2
- This was found to confer a transmission advantage by stabilizing the conformation of the spike towards a state favorable for binding the host angiotensin-converting enzyme 2 (ACE2) receptor. (news-medical.net)
- some PSA escapes the prostate and can be found in the serum. (medscape.com)
Variations1
- At both time points, D614G and B.1.1.7 strains showed equal sensitivity to the sera, but with large inter-individual variations over two orders of magnitude. (news-medical.net)
Study1
- Thus, the aims of the current study are (1) to compare the differences in serum indicators related to iron stores in the body between CD patients and controls and (2) to investigate whether these indicators are associated with disease severity. (nature.com)
Ratio2
- MDI conjugation was observed to be dependent on the MDI: protein ratio and the concentration of protein. (cdc.gov)
- Greater binding to HSA than Hb was observed and MDI bound to only eight binding sites on Hb compared to twenty for HSA (at 40:1 molar ratio of MDI: protein). (cdc.gov)
Blood3
- In blood circulation, over 75% of IGF-I is bound to the insulin-like growth factor binding protein-3 (IGFBP-3). (e-enm.org)
- The serum iron test measures the amount of iron in your blood. (rochester.edu)
- This is the protein in your red blood cells that carries oxygen around your body. (rochester.edu)
Samples2
- Serum samples from 354 subjects (180 male, 174 female) were analyzed based on sex at 10-year intervals from 21 to 70 years. (e-enm.org)
- Salimetrics has not validated this kit for serum or plasma samples. (salimetrics.com)
Total1
- They showed that the level of serum iron and the total iron binding capacity were decreased in active CD patients due to impaired oral iron absorption 8 . (nature.com)
Healthy1
Complex1
- The C1 macromolecular complex, C1qC1r2C1s2, is bound together by Ca2+ ions. (bdbiosciences.com)
Range1
- Serum protein binding is constant over the concentration range (0.15-105 g/mL) achieved with recommended doses. (nih.gov)
Activity1
- Neutralizing activity was more potent in sera from those with critical disease relative to all others, but was maintained in all cases at six months. (news-medical.net)
Growth1
- IFABP and the binding thermodynamics (Richieri growth and differentiation (Glatz & van der et al. (lu.se)
Specific1
- Prostate-specific antigen (PSA) is a protein produced by normal prostate cells. (medscape.com)
Body1
- The central body of the alpha chain homopolymer supports tentacles, each with the binding site for C4b at the end. (lu.se)
Cells1
- Target cells for MAC action may be heterologous erythrocytes, nucleated cells (autologous or foreign), bacteria (Gram-negative, susceptible to serum), microscopic fungi, viruses with a surface envelope and virus-infected cells. (dadamo.com)