• The immunogens included the intact pyruvate dehydrogenase complex (PDC) from bovine heart, human recombinant PDC-E2, and short synthetic peptides representing the immunodominant lipoic acid binding sequences of the 2-OADC enzymes. (edu.au)
  • The techniques for antibody analysis included immunofluorescence, immunoblotting on mitochondrial extracts, ELISAs using entire PDC, PDC-E2, or synthetic peptides, epitope mapping by peptide scanning on overlapping octameric peptides representing the human PDC-E2 sequence, affinity purification on PDC-E2, and inhibition in vitro by sera of the catalytic function of PDC. (edu.au)
  • Antibodies to cyclic peptides containing citrullinated sites were screened in sera from patients with RA by ELISA. (ox.ac.uk)
  • Two immunodominant peptides cTNC1 (VFLRRKNG-cit-ENFYQNW) and cTNC5 (EHSIQFAEMKL-cit-PSNF-cit-NLEG-cit-cit-KR) were identified. (ox.ac.uk)
  • Antibodies to both showed limited cross-reactivity with ACPA epitopes from α-enolase, vimentin and fibrinogen, and no reactivity with citrullinated fibrinogen peptides sharing sequence homology with FBG. (ox.ac.uk)
  • For the majority of these peptides, species-specific seroreactivity in mammalian sera has been reported in literature. (biomedcentral.com)
  • In field sera, signals were difficult to interpret as insufficient sera from experimentally infected chickens were available for evaluating the seroreactivity of all peptides. (biomedcentral.com)
  • The goal of this study is to investigate if it is possible to develop a serological test that, in chicken serum, can differentiate between antibodies against different Chlamydia species, particularly C. psittaci and C. gallinacea , using peptides as antigens. (biomedcentral.com)
  • Anti-THSD7A positive sera were further characterized by western blotting and slot blotting on THSD7A protein fragments and peptides. (manchester.ac.uk)
  • Peptides were synthesized and tested for in vivo immunogenicity by screening peripheral blood mononuclear cells from healthy volunteers (n = 64) and HAdV-infected stem cell recipients (n = 26) for memory T cells recognizing the candidate epitopes in the context of most common HLA alleles. (biomedcentral.com)
  • Functional CD8 + T cells recognizing seven epitopes were identified, among them four penton-derived and two hexon-derived peptides. (biomedcentral.com)
  • Interleukin-4-specific responses, both ex vivo and cultured to Der p 2 peptides, had a significant positive correlation with HDM-specific serum IgE. (ox.ac.uk)
  • The epitope profile of vaccinated individuals differed from convalescents, with a more diverse repertoire of immunodominant peptides, recognized by more than 30% of the cohort. (bvsalud.org)
  • The chip contains the sequences of 15 different capsid proteins converted into more than 5,000 overlapping peptides for high-resolution epitope data. (pepperprint.com)
  • Available with linear or cyclic constrained peptides for linear or conformational epitope mapping, the PEPperCHIP ® Pan-AAV Capsid Protein Microarray can be used to analyze patient and animal sera as well as research and diagnostic anti-AAV antibodies on the epitope level. (pepperprint.com)
  • furthermore, in conjunction with the usage of validated bioinformatic equipment the prediction may raise the probability of locating promising focuses on MS-275 (Entinostat) for immune reputation of SARS-CoV-2.30 Another technique for determining linear B cell epitopes is to display man made overlapping peptides using the elicited neutralizing antibodies in convalescent individuals. (giknet.org)
  • Thus, fragment C of spike protein was identified as an immunodominant antigen and could be used for serological detection of SARS-CoV infection. (nus.edu.sg)
  • Labsystems HIV Ag-Ab ELISA KIT is a qualitative immunoassay for detection of antigen & antibodies of HIV-1 & HIV-2 in Human Serum or Plasma. (trivitron.com)
  • Sandwich ELISA for detection of antibody against HIV 1 and HIV 2 along with detection of p24 antigen in Human Serum or Plasma. (trivitron.com)
  • However, the usefulness of existing simple immunoassay formats is limited by technical considerations such as sera antibodies to contaminants in insufficiently pure antigen, a problem likely exacerbated when antigen panels are screened to obtain clinically useful data. (biomedcentral.com)
  • We developed a novel and simple immunoprecipitation technology for identifying clinical sera containing antigen-specific antibodies and for generating quantitative antibody response profiles. (biomedcentral.com)
  • After mixing crude extracts, sera and protein A/G beads together and incubating, during which the Ruc-antigen fusion become immobilized on the A/G beads, antigen-specific antibody is quantitated by washing the beads and adding coelenterazine substrate and measuring light production. (biomedcentral.com)
  • Tests employing bacterial-produced proteins can produce high backgrounds because it is difficult to completely eliminate or block serum antibodies reactive with trace amounts of bacterial contaminants present in most antigen preparations, even in pharmaceutical grade preparations [ 3 ]. (biomedcentral.com)
  • Identification of an immunodominant region of the major house dust mite allergen Der p 2 presented by common human leucocyte antigen alleles. (ox.ac.uk)
  • AIM: To identify CD4(+) T-cell epitopes within Der p 2 recognized by commonly expressed human leucocyte antigen (HLA) alleles. (ox.ac.uk)
  • A 125-kD surface antigen of Entamoeba histolytica is recognized by 73% of immune sera from patients with amoebic liver abscesses. (silverchair.com)
  • Using pooled human immune sera a cDNA clone (lambda cM17) encoding this antigen (M17) has been isolated from a lambda gt11 expression library of the virulent stain E. histolytica HM1:IMSS. (silverchair.com)
  • In contrast to conservation of an immunodominant epitope recognized in pathogenic and nonpathogenic strains by monoclonal FA7 and human immune sera, amplification and sequence analysis of a 1,4000-bp fragment of this gene from a fresh nonpathogenic isolate by use of the PCR demonstrate regions of significant sequence divergence in this antigen. (silverchair.com)
  • The immunodominant antigen of P. brasiliensis is the glycoprotein gp43, which is recognized by approximately 100% of the sera from patients with PCM, making it an important tool for the diagnosis of disease. (fapesp.br)
  • A novel cELISA was established and optimized based on the strategy that 6B211 can compete with C-strain induced neutralizing antibodies in pig serum to bind capture antigen C-strain E2. (biomedcentral.com)
  • This assay allows screening of specific antibodies of any class with the sandwich Enzyme Immunoassay (EIA) method using immunodominant recombinant antigens Tp15, Tp17 and Tp47. (adaltis.net)
  • Recombinant Antigens representing immunodominant epitopes of Treponema pallidum (Tp15, Tp17 and Tp47) are coated onto wells of a microplate. (adaltis.net)
  • Serum or plasma specimens and Assay Diluent are added to these wells, followed by a solution of biotinylated antigens. (adaltis.net)
  • The Labsystems HIV Ag-Ab ELISA Kit is a 4th generation in vitro diagnostic immunoassay for the qualitative determination of antigens & antibodies to HIV-1, HIV-1 group O, and HIV-2 in human serum or plasma. (trivitron.com)
  • The results of competition experiments, preincubating positive sera with unmodified E. coli -produced antigens, varied dramatically. (biomedcentral.com)
  • Serum antimitochondrial antibody targets non-organ-specific mitochondrial antigens numerically classified as M1-M9. (medscape.com)
  • A total of 1,119 serum samples collected between March and July of 2020 from health employees and visitors to the University Hospital at the University of São Paulo were screened with the Elecsys® Anti-SARS-CoV-2 immunoassay (Elecsys) (Roche Diagnostics) and three in-house ELISAs that are based on different antigens: the Nucleoprotein (N-ELISA), the Receptor Binding Domain (RBD-ELISA), and a portion of the S1 protein (ΔS1-ELISA). (bvsalud.org)
  • Identify, profile, and compare AAV-specific IgG, IgA, and IgM responses in patient or animal sera for vaccine development, or screen viral antigens to find and characterize immunodominant epitopes for in-vitro diagnostics research. (pepperprint.com)
  • Custom peptide arrays will be constructed to identify immunodominant epitopes in human serum, which we will then optimize as an ELISA-based diagnostic for use in developing countries. (jcvi.org)
  • Moreover, the experimentally-induced antibodies in striking contrast to the natural autoantibodies showed preferential reactivity with PDC-E2 rather than with intact PDC, failed to inhibit in vitro the catalytic function of PDC, and, on peptide scanning, reacted with discrete epitopes, but at sites other than the lipoyl-binding region of PDC-E2. (edu.au)
  • We constructed IDEs-RFP plasmids and prepared anti-IDEs peptide mouse sera to identify IDEs through immunological tests. (biomedcentral.com)
  • A neutralization test in vitro showed that the peptide-specific mouse antisera of IDE4 (242-256 aa) and HN341-355, a reported neutralizing linear epitope, could partially neutralize avirulent LaSota as well as virulent strains at similar levels, suggesting that IDE4 might be a potential neutralizing linear epitope. (biomedcentral.com)
  • Among the methods for identifying epitopes, peptide scanning technology, such as pepscan, has been widely used due to its high sensitivity and rapidity [ 19 ]. (biomedcentral.com)
  • Identification of an immunodominant peptide from citrullinated tenascin-C as a major target for autoantibodies in rheumatoid arthritis. (ox.ac.uk)
  • The autoantibody response to one immunodominant cTNC peptide was then analysed in 101 pre-RA sera (median 7 years before onset) and two large independent RA cohorts. (ox.ac.uk)
  • METHODS: HLA-blocking antibodies, peptide pools and truncations were used in ELISpot assays to establish restricted T-cell epitopes. (ox.ac.uk)
  • Identification of such epitopes may be of future value in peptide immunotherapeutic approaches. (ox.ac.uk)
  • Methods: We used an RBD peptide microarray to identify IgG and IgA binding regions in serum of 71 COVID-19 convalescents and 18 vaccinated individuals. (bvsalud.org)
  • MS-275 (Entinostat) For instance, Poh et al reported that two IgG immunodominant areas which can be found near RBD with the fusion peptide, respectively, added to virus neutralization capacities of convalescent sera significantly. (giknet.org)
  • The presence of serum antimitochondrial antibodies (AMA) is a highly specific indication of primary biliary cirrhosis (PBC). (medscape.com)
  • Detectable serum antimitochondrial antibody (AMA) with a titer over 1:40 is used as laboratory testing support of the diagnosis of primary biliary cirrhosis (PBC). (medscape.com)
  • Even among asymptomatic patients, a positive serum antimitochondrial antibody result is highly suggestive of primary biliary cirrhosis or indicates of significant risk of developing active primary biliary cirrhosis in succeeding years. (medscape.com)
  • 8] In addition, 5%-10% of patients with primary biliary cirrhosis are reported to have no detectable level of serum antimitochondrial antibody. (medscape.com)
  • Among recipients of liver transplants for end-stage primary biliary cirrhosis, the serum antimitochondrial antibody level can decrease slightly and elevate back to pretransplant levels in the first year posttransplant. (medscape.com)
  • Nineteen candidate nonamers from hexon and penton proteins were identified by epitope binding prediction. (biomedcentral.com)
  • We show that 20-85% of these sera contain significant titers of antibodies against at least one of five frequently mutated and/or overexpressed tumor-associated proteins. (biomedcentral.com)
  • In addition, it could also reveal a possible encouragement technique to current vaccines through highlighting neutralizing epitopes that are underprivileged for antigenicity demonstration in indigenous S proteins. (giknet.org)
  • Identification of new immunodominant epitopes will facilitate and broaden immune assessment strategies to identify patients suitable for T-cell transfer. (biomedcentral.com)
  • Identification of a dominant epitope of glutamic acid decarboxylase (GAD-65) recognized by autoantibodies in stiff-man syndrome. (silverchair.com)
  • Introduction: Considering the likely need for the development of novel effective vaccines adapted to emerging relevant CoV-2 variants, the increasing knowledge of epitope recognition profile among convalescents and afterwards vaccinated with identification of immunodominant regions may provide important information. (bvsalud.org)
  • The fact that P. lutzii isolates belonging to different phylogenetic groups suggests that these approaches to identification of the fungus, and development of serological diagnosis based vaccines only in gp43 and its immunoprotective epitope P10 may not be effective for all isolates. (fapesp.br)
  • The immunodominant epitope for these autoantibodies is associated with the lipoyl-binding domain of the E2 subunit of the enzymes. (edu.au)
  • CONCLUSIONS: There are multiple citrullination sites in the FBG domain of tenascin-C. Among these, one epitope is recognised by autoantibodies that are detected years before disease onset, and which may serve as a useful biomarker to identify ACPA-positive patients with high sensitivity and specificity in established disease. (ox.ac.uk)
  • Nevertheless, because these strategies do not have the required functional sensitivity, possess a complex and manual workflow, and aren't universally available, Tg measurements with LCMS MS really should be restricted for TgAbpositive serum samples Therefore, there is certainly apparently Lys-Ile-Pro-Tyr-Ile-Leu SCD inhibitor 1 Nevertheless a require for any highly sensitive Tg immunoassay with significantly less interference by Tg autoantibodies. (adenosine-kinase.com)
  • Having said that, for the reason that these solutions usually do not possess the needed functional sensitivity, possess a complicated and manual workflow, and aren't universally readily available, Tg measurements with LCMS MS really should be restricted for TgAbpositive serum samples Thus, there is certainly apparently nevertheless a will need for a hugely sensitive Tg immunoassay with much less interference by Tg autoantibodies. (adenosine-kinase.com)
  • Our research provides potential epitope delivery and applicants technique for the introduction of a multi-epitope based SARS-COV-2 vaccine. (giknet.org)
  • The best multi-epitope of the possible immunogenic property was created by combining epitopes. (springeropen.com)
  • BACKGROUND: Better understanding of the relevance of the immune response to common environmental allergens, such as the major house dust mite (HDM) allergen Der p 2, requires characterization of constituent T-cell epitopes. (ox.ac.uk)
  • MASPpep encompasses B- and T-cell epitopes revealed by proteomics and immunoinformatics. (utep.edu)
  • Bioinformatics techniques are used to predict B cell and T cell epitopes from the new SARS-CoV-2 spike glycoprotein in order to build a unique multiple epitope vaccine. (springeropen.com)
  • Induction of binding Abs was measured by ELISA and neutralizing activity by PBMC and TZM-bl assays in all serum and vaginal samples. (europa.eu)
  • Potential cross-reactivity with well-established anticitrullinated protein antibody (ACPA) epitopes was tested by inhibition assays. (ox.ac.uk)
  • Hyperimmune mouse serum raised against purified, recombinant OmpP4 did not promote bactericidal killing of 35000HP or phagocytosis by J774A.1 mouse macrophages in serum bactericidal and phagocytosis assays, respectively. (biomedcentral.com)
  • Similar patterns were observed for spike protein-specific serum antibodies and T cells, but the magnitude of each of these responses was diminished relative to the control cohort. (nature.com)
  • Circulating antibodies and T cells are directed against an epitope located in the extracellular region of BPAG2 near the membrane, called the MCW-1 domain. (medscape.com)
  • This fragment was highly expressed, and the purified fragment C could detect all 33 SARS patient serum samples tested, collected from 7 to 60 days after the onset of fever, but had no reactivity with all 66 healthy human serum samples tested. (nus.edu.sg)
  • Reactivity to this sequence was lost following kallikrein protease cleavage within the predicted epitope. (manchester.ac.uk)
  • This reactivity is similar to that displayed by the monoclonal antibody GAD 6, suggesting the presence of a single immunodominant epitope (SMS-E1) in this region of GAD-65. (silverchair.com)
  • To study cellular immune reactivity to CII in patients with RA and in healthy control individuals and to correlate this reactivity to HLA class II genotypes and to the presence of antibodies to CII in serum. (biomedcentral.com)
  • The EIAgen Syphilis Ab Kit assay, is a third generation solid phase enzyme immunoassay for the qualitative detection of antibodies to Treponema pallidum (TP) in human sera or plasma (EDTA, Heparin Lithium, Sodium Citrate). (adaltis.net)
  • Sera from each period ( n = 185 and n = 693) were screened in an epitope blocking enzyme immunoassay for flavivirus antibody detection. (who.int)
  • A recombinant form of PDC is cloned and used to detect the presence of AMA in serum via EIA. (medscape.com)
  • Other specific initiatives were conducted such as evaluation of the sublingual route for immunization, investigation of the Principal Immunodominant Domain (PID) of gp41, and direct HIV inhibition with an Ag family which demonstrated in vitro antiviral activities comparable to the antiretroviral drug T20 (Fuzeon). (europa.eu)
  • However, its antigenic epitopes have not been fully elucidated. (biomedcentral.com)
  • Therefore, defining the linear epitopes of HN, especially neutralizing epitopes, will be useful for revealing its antigenic characterization. (biomedcentral.com)
  • These results will help elucidate the antigenic epitopes of the HN and facilitate the development of NDV vaccines. (biomedcentral.com)
  • However, the linear antigenic epitopes of HN have not been fully elucidated. (biomedcentral.com)
  • B-cell epitope prediction analysis and homology modelling revealed this sequence to be antigenic and surface available suggesting it is accessible for the antibody to bind. (manchester.ac.uk)
  • demonstrated that pemphigoid gestationis sera recognize five distinct epitopes within BP180 NC16A, four of which have been reported as major antigenic sites targeted by bullous pemphigoid antibodies. (medscape.com)
  • Fifty-one vaccine candidates were designed mainly to mimic fusion intermediate conformations of gp41 and in some instance to increase exposure of the 2F5/4E10 epitopes. (europa.eu)
  • The vaccine was safe and well tolerated, induced potent serum IgG & IgA responses waning post last vaccination, and low and intermittent mucosal IgG, but did not induce neutralizing activity neither in serum nor vaginal samples. (europa.eu)
  • In this study, we analyzed B-cell immunodominant epitopes (IDEs) of the HN protein from the vaccine strain LaSota using pepscan technology with LaSota-specific chicken hyperimmune antisera. (biomedcentral.com)
  • P10 is an epitope from gp43, and studies have shown that it can acts as a vaccine against PCM in mice. (fapesp.br)
  • Subsequently, we show the efficacy of the T. cruzi immunodominant glycotope Galα3LN, covalently linked to a carrier protein (human serum albumin, HSA), as a prophylactic vaccine candidate in the acute model of ChD. (utep.edu)
  • This research is entirely reliant on the development of epitope-based vaccines, and these in silico findings would represent a major step forward in the development of a vaccine that might eradicate SARS-CoV-2 in Bangladeshi patients. (springeropen.com)
  • PAL is conserved among H. ducreyi strains and contains a surface-exposed epitope defined by the monoclonal antibody 3B9 [ 8 ]. (biomedcentral.com)
  • We aimed at identifying further targets of protective anti-HAdV immune response and characterizing immunogenic epitopes. (biomedcentral.com)
  • We examined the relationship between peripheral blood levels of SARS-CoV-2 S (Spike protein)1/M (Membrane protein)-reactive IFN-γ-producing CD4 + and CD8 + T cells, serum levels of biomarkers of clinical severity, and mortality in critically ill COVID-19 patients. (nature.com)
  • Despite the fact that a global vaccination program is on the spot, novel lineages comprising a cluster of mutations in the receptor-binding domain and immunodominant areas of the N terminal domain of spike glycoprotein have developed, resulting in immunological escape variants with higher transmissibility and infectivity [ 4 ]. (springeropen.com)
  • The effect of the D614G change on the conformation of the spike as well as the identity and nature of immunodominant epitopes are still unknown. (umn.edu)
  • In the SARS-CoV spike, the equivalent D614 residue is embedded in an immunodominant epitope but the ability of this region in SARS-CoV-2 spike to elicit an antibody response is still not known. (umn.edu)
  • 38 Neutralisation tests or an epitope-blocking ELISA can help identify MVEV-specific antibodies. (mja.com.au)
  • With the commercial molecules synthesized on the basis of these designs, we tested 86 serum samples from 42 mother/newborn pairs and two congenitally infected newborns, by indirect ELISA. (frontiersin.org)
  • To find anti-THSD7A sera for this study, we screened 1843 sera from biopsy-proven MN patients by ELISA and identified 22 sera as anti-THSD7A positive representing 1.2% of MN cases. (manchester.ac.uk)
  • Twenty-six of these patients who had low levels of anti-CII in serum were randomly chosen, whereas 19 patients with high anti-CII levels were identified by enzyme-linked immunosorbent assay (ELISA)-screening of 400 RA sera. (biomedcentral.com)
  • Following HIV-1 infection, serum neutralizing antibody responses against the evolving autologous viral swarm are generated by the vast majority of infected subjects, usually within the first few months of infection [ 1 - 6 ]. (plos.org)
  • Five of six colon cancer sera tested gave responses that were statistically significantly greater than the average plus three standard deviations of 10 control sera. (biomedcentral.com)
  • The immunogenicity, antigenicity scores, and toxicity of these epitopes were evaluated and chosen based on their capacity to elicit an immune response. (springeropen.com)
  • All ten selected sera bound to the T28mer confirming this sequence as a dominant epitope in THSD7A. (manchester.ac.uk)
  • As the use of serological methods increases the window of detection, the goal of this study was to investigate if it is possible to develop a differentiating serological test for antibodies against Chlamydia species in chicken sera. (biomedcentral.com)
  • Patient or animal serum is incubated on the microarray and antibodies present in the sample bind their corresponding epitopes on the microarray surface. (pepperprint.com)
  • All patient sera contained antibodies that recognize strongly GAD-65, but also GAD-67, when tested by immunoprecipitation on brain extracts and by immunoprecipitation or immunocytochemistry on cells transfected with either the GAD-65 or the GAD-67 gene. (silverchair.com)
  • To gain insight into the potential threat for the population of Europe, we determined the neutralizing activity in intravenous immunoglobulin (IVIg) batches and individual serum samples from donors in the Netherlands against EV-71 strains isolated in Europe and in Asia. (cdc.gov)
  • These studies have mostly been conducted by using serum samples from Asian donors or animals immunized with Asian outbreak strains. (cdc.gov)
  • JCVI is currently working with collaborators in Colombia and Nicaragua to collect sera from patients suspected to harbor ZIKV and to sequence the viral genome from these patients. (jcvi.org)
  • cTNC5 antibodies were detected in 18% of pre-RA sera, and in 47% of 1985 Swedish patients with RA and 51% of 287 North American patients with RA. (ox.ac.uk)
  • We have characterized this technology with sera from patients having three different types of cancers. (biomedcentral.com)
  • 11] In addition, a minority of patients with fulminant hepatic failure from various causes, such as drug-induced liver damage and hepatitis A and B, are reported to have transiently detectable levels of serum antimitochondrial antibody that disappear within one year. (medscape.com)
  • The immunodominant tGPI-mucin α-Gal glycotope, the trisaccharide Galα1,3Galβ1,4GlcNAc (Galα3LN), elicits high levels of protective T. cruzi-specific anti-α-Gal antibodies in ChD patients, in both the acute and chronic phases. (utep.edu)
  • PATIENTS: 59 consecutive patients with chronic hepatitis C. MEASUREMENTS: Hepatitis C virus serotype was determined using a recently developed immunoenzymatic assay that detects antibodies directed to serotype-specific immunodominant epitopes. (ox.ac.uk)
  • Rheumatoid arthritis patients with elevated serum levels of immunoglobulin (Ig)G anti-CII antibodies had lower CII-induced IFN-γ production than patients with low anti-CII levels. (biomedcentral.com)
  • In certain cases, it shows total immunological escape in convalescent serum samples, raising concerns about the effectiveness and efficacy of currently available vaccinations [ 5 ]. (springeropen.com)
  • Over the upcoming year, JCVI anticipates screening both retrospective and prospective human serum samples for ZIKV, with the assumption that many of these samples are from individuals infected with other viral diseases (e.g. (jcvi.org)
  • All IVIg batches and 41%, 79%, and 65% of serum samples from children ≤5 years of age, women of childbearing age, and HIV-positive men, respectively, showed high neutralizing activity against a Dutch C1 strain, confirming widespread circulation of EV-71 in the Netherlands. (cdc.gov)
  • In the first season, testing for antibodies to specific alphaviruses was conducted on samples with sufficient sera ( n = 22). (who.int)
  • The serological diagnosis of syphilis is performed demonstrating the presence of significant levels of specific T. pallidum antibodies in the patient serum or plasma sample. (adaltis.net)
  • Amino acid sequence analysis showed that the neutralizing epitope and T cell epitope of PRRSV-1, and the decoy epitope region and hypervariable regions of PRRSV-2 had evolved under positive selection pressure. (researchsquare.com)
  • This region of BPAG2 is also an immunodominant epitope in bullous pemphigoid, a closely related autoimmune blistering disease. (medscape.com)
  • When tested by Western blotting, all patient sera selectively recognized GAD-65. (silverchair.com)
  • Surface binding and cap formation are observed with patient sera, purified monospecific antiserum, and mAb FA7. (silverchair.com)
  • Noteworthy, immunodominant regions of recognition by vaccinated coincide with mutation sites at Omicron BA.1, an important variant emerging after massive vaccination. (bvsalud.org)
  • The EIAgen Syphilis Kit is based on the 'sandwich' principle, a solid-phase enzyme-linked immunoassay technique, to measure anti- Treponema pallidum levels in serum or plasma. (adaltis.net)
  • Can be assayed with at least 20 µL serum/plasma or 10-20 µg/mL purified antibody. (pepperprint.com)
  • Nevertheless, results of the suspension array with field sera are supported by published data on the occurrence of C. gallinacea in Dutch layers, thereby demonstrating the proof of concept of multiplex serology for Chlamydial species in poultry. (biomedcentral.com)
  • This array format is characterized by the addition of a carrier protein or polypeptide forming glycoconjugate-based epitopes. (srcinhibitors.com)
  • for instance by applying bovine serum albumin as carrier protein Inhibitors,research,lifescience,medical to epoxide-derivatized slides [61]. (srcinhibitors.com)
  • The former encompasses the trisaccharide Galα1,3Galβ1,4GlcNAcα (Galα3LNα), an immunodominant glycotope found on the trypomastigote mucins, coupled to a maleimide-modified human serum albumin as carrier protein. (utep.edu)
  • From these 23 fragments, we identified a fragment of 259 amino acids (amino acids 441 to 700) that is a major immunodominant epitope. (nus.edu.sg)
  • All SMS sera strongly recognized a fragment contained between amino acid 475 and the COOH terminus (amino acid 585). (silverchair.com)
  • In addition, most SMS sera recognized at least one epitope (SMS-E2) in the NH2-terminal domain of GAD-65 (amino acids 1-95). (silverchair.com)
  • We tested sera by RT-PCR to detect dengue virus (DENV) and identify serotypes. (cdc.gov)
  • No correlation was found between SARS-CoV-2 IFN-γ T-cell counts, anti-RBD IgG concentrations and biomarker serum levels (Rho ≤ 0.3). (nature.com)
  • A signature motif in the N-terminus domain of THSD7A (T28mer) with sequence homology to the major PLA2R epitope (P28mer) was identified. (manchester.ac.uk)
  • Real time interaction analyses and antibodies off-rate could be reliably determined using bio-layer interferometry.A signature motif in the N-terminus domain of THSD7A (T28mer) with sequence homology to the major PLA2R epitope (P28mer) was identified. (manchester.ac.uk)
  • The HAdV-derived protein hexon has been recognized as a major immunodominant target across HAdV species. (biomedcentral.com)
  • From their crossinhibition patterns, these antibodies have been classified into 5 epitope groups, A to E, where group D is comprised by two subgroups. (adenosine-kinase.com)
  • In 1917, a 12-year-old with features suggestive of MVEV infection recovered after receiving immune serum. (mja.com.au)
  • The potential association between SARS-CoV-2-S-Receptor Binding Domain (RBD)-specific IgG levels in sera and mortality was also investigated. (nature.com)
  • Differing epitope selection of experimentally-induced and natural antibodies to a disease-specific a. (edu.au)
  • Differing epitope selection of experimentally-induced and natural antibodies to a disease-specific autoantigen, the E2 subunit of pyruvate dehydrogenase complex (PDC-E2). (edu.au)
  • Another platform utilizes an identified cancer-specific immunodominant glycopeptide epitope in MUC1 [64], a heavily glycosylated mucin known to be Inhibitors,research,lifescience,medical associated to several cancer types including breast and ovarian cancer. (srcinhibitors.com)