• Lastly, these potato events express a resistance protein (R-protein) found in wild Solanum species Solanum venturii and Solanum phureja (VNT1). (canada.ca)
  • it's thought that up to three-quarters of the world's insect species remain undescribed. (cosmosmagazine.com)
  • Pollination by the European honeybee alone is estimated to be worth $4-6 billion per year in Australia, and that's only one of the estimated one million documented species of insects living on Earth. (cosmosmagazine.com)
  • Of course, many native insect species like flies, moths and beetles help pollinate some of Australia's most iconic plants like Eucalyptus and tea trees. (cosmosmagazine.com)
  • What attracted me to entomology, the study of insects, is that there are so many species still waiting to be discovered to science, each potentially with their own important role in the ecosystem or answer to the next challenge that we might face. (cosmosmagazine.com)
  • Scientists estimate that we've only documented one quarter of the world's insect species, so it's no surprise that we are discovering new species in tropical rainforests or even our own backyard. (cosmosmagazine.com)
  • Part of my research was naming new species and using DNA sequencing to look back in time and reconstruct the global migration of horse flies throughout the world. (cosmosmagazine.com)
  • It was no surprise that my collaborator, Norman Woodley from the Smithsonian, and I discovered close to 150 new species already sitting in the CSIRO Australian National Insect Collection and state museums. (cosmosmagazine.com)
  • Now I'm working on mosquitoes, trying to improve the diagnostic capabilities of surveillance officers to more rapidly and accurately identify Australian species using high throughput genome sequencing of museum specimens. (cosmosmagazine.com)
  • The inotocin/receptor couple can also be found in the recently sequenced genome from the parasitic wasp Nasonia vitripennis but not in any other holometabolous insect with a completely sequenced genome (12 Drosophila species, the malaria mosquito Anopheles gambiae, the yellow fever mosquito Aedes aegypti, the silk worm Bombyx mori, and the honey bee Apis mellifera), suggesting that this neuropeptide system is confined to basal holometabolous insects. (lu.se)
  • Because it's genome has been sequenced, its genetics are easily manipulated, and it is easy to maintain in the lab, this species of yeast has been an invaluable resource in the understanding of fundamental cellular processes such as cell division and cell death. (jove.com)
  • To overcome the host plant defense response, M. phaseolina encodes a significant number of P450s, MFS type membrane transporters, glycosidases, transposases, and secondary metabolites in comparison to all sequenced ascomycete species. (biomedcentral.com)
  • Pairwise amino acid identities to EILV ranged between 67 and 88 % for the corresponding proteins, suggesting that TALV defines a proposed new alphavirus species. (microbiologyresearch.org)
  • Among the insects, only the dipterans ( Drosophila and various mosquito species) have been widely investigated for their immune responses towards diverse pathogens. (biomedcentral.com)
  • Several genes were present as members of gene families, as has also been observed in other insect species. (biomedcentral.com)
  • The reports on base sequences of spider silk protein genes have gained importance as the mechanical properties of silk fibers have been revealed. (bioone.org)
  • With the exception of late blight resistance, the other novel traits (i.e. reduced asparagine levels, lower levels of reducing sugars, and reduced black spot bruising) are achieved through the transcription of inverted repeat sequences containing small fragments of DNA from five different endogenous genes (i.e. (canada.ca)
  • Asn1 , Ppo5 , PhL , R1 , and VInv ), which results in the reduced level of mRNA transcripts (and subsequently expressed proteins) for those same genes using the RNA interference (RNAi) pathway. (canada.ca)
  • The sequencing effort identified more than 45,000 putative protein-coding genes in the Populus genome. (bioedonline.org)
  • The analysis uncovered transcripts coinciding with thousands of protein-coding genes, including 636 genes believed to code for secreted proteins found in insect saliva. (genomeweb.com)
  • The present study has identified many potential immune-related genes in A. mylitta some of which are vertebrate homologues and others are hitherto unreported putative defence proteins. (biomedcentral.com)
  • With reference to insect immunity, the ESTs have been obtained from baculovirus-infected B. mori cultured cells and pupae, but no large scale information on bacteria-induced immune genes is as yet available. (biomedcentral.com)
  • We resolve chromosomal sequences and infer sex chromosome-specific parasite genes and new potential drug targets. (nature.com)
  • The pQE-TriSystem vector contains CAG, T5, and p10 promoters that enable 6xHis-tagged protein expression in mammalian, E. coli , and baculovirus-infected insect cells, respectively (see figure pQE TriSystem. pQE TriSystem. "> pQE TriSystem ). (qiagen.com)
  • This review aims to link recent molecular data, often translated into amino acid sequences and predicted three dimensional structural motifs, to known mechanical properties. (bioone.org)
  • Methods: Thirty-two degenerate oligonucleotides DNA primers of 24 nucleotides each were synthesized based on the published amino acid sequences of peanut BBTI. (usda.gov)
  • Two pairs of degenerate primers were designed based Acalisib IC50 on the conserved amino acid sequences of chemosensory proteins from different Lepidoptera bugs. (woofahs.com)
  • PEPCF expresses proteins in bacteria, insect and mammalian cells and uses a variety of chromatographic and biophysical techniques for protein purification and characterization. (embl.org)
  • In case expression constructs and/or protocols are already present, you can immediately fill out our protein expression and purification request form and send the completed form back to us. (embl.org)
  • After we receive the sequence-verified expression constructs, we will first perform small scale expression and purification tests in which we assess the expression level, the solubility and the protein enrichment after a small scale affinity purification. (embl.org)
  • Once suitable conditions have been identified, we proceed to large scale expression (1-6 liter per construct) and protein purification using a variety of chromatographic techniques. (embl.org)
  • After harvesting the V1 virus, we perform small scale expression and purification tests, in which we assess the expression level, the solubility and the protein enrichment after a small scale affinity purification. (embl.org)
  • Finally, the video describes some of the many ways in which yeast cells are put to work in modern scientific research, including protein purification and the study of DNA repair mechanisms and other cellular processes related to Alzheimer's and Parkinson's diseases. (jove.com)
  • In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization). (antibodies-online.com)
  • In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. (antibodies-online.com)
  • Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. (antibodies-online.com)
  • Vector pQE-TriSystem constructs can be transformed into E. coli , used as a shuttle vector for recombinant protein expression in insect cells, or transfected into mammalian cells. (qiagen.com)
  • S. cerevisiae is an attractive model organism due to the fact that its genome has been sequenced, its genetics are easily manipulated, and it is very easy to maintain in the lab. (jove.com)
  • In addition, we use bioinformatic analysis across twelve Drosophila genomes to identify putative cis-regulatory sequences that may be capable of facilitating specific promoter-enhancer interactions at the bithorax complex and propose a model for their molecular function during development. (nih.gov)
  • Results: Three putative positive clones were isolated, purified, subcloned and sequenced. (usda.gov)
  • Of the four putative novel defence proteins (DFPs) annotated by PSI-BLAST three showed similarity to extracellular matrix proteins from vertebrates implicated in innate immunity, while the fourth was similar to, yet distinct from, the anti-microbial protein cecropin. (biomedcentral.com)
  • Sequence analysis revealed a partial cDNA clone of 643 bp with a start codon and with 96% and 93% homology with peanut allergens Ara h 4 and Ara h 3 cDNA clones, respectively. (usda.gov)
  • Although their specific function remains uncertain, OS-D-like members have been isolated from sensory organs (including the sensillum lymph in some cases), and a role in olfaction similar to that of the insect odorant binding proteins (OBP) has been suggested for some.We have identified 15 new OS-Dlike sequences: four from cDNA clones described herein and 11 from sequencedatabases. (gc.ca)
  • As described in Materials and Methods, we constructed a cDNA library from fat body tissues of E. coli - challenged A. mylitta larvae and randomly sequenced a large number of inserts from the library. (biomedcentral.com)
  • We offer services for DNA and cDNA (RNA-Seq) sequencing. (lu.se)
  • When a cell needs to make a particular protein, it first copies the instructions from the matching gene into a molecule known as a messenger RNA (or an mRNA for short). (elifesciences.org)
  • Use of Agrobacterium and baculoviruses for gene expression in plant and insect cells respectively. (up.ac.za)
  • Roughly 25.4% of the whole-insect genome appears to encode long interspersed nuclear elements (LINEs), and a total of 11,901 protein sequences predicted by automated gene finding exhibited homology with reference sequences in the NCBI NR and/or UniProtKB databases at the most stringent similarity cutoff level (i.e., the gold tier). (usda.gov)
  • The search led to a homozygous, missense mutation in the vacuole protein-sorting gene VPS11 that subsequently turned up in other children with leukoencephalopathy. (genomeweb.com)
  • Analysis of the insect os-d-like gene family. (gc.ca)
  • Here, we report that the recently sequenced genome from the red flour beetle Tribolium castaneum contains a gene coding for an oxytocin/vasopressin-like peptide, identical to the locust peptide, which we named inotocin (for insect oxytocin/vasopressin-like peptide) and a gene coding for an inotocin G protein-coupled. (lu.se)
  • Because many yeast proteins are similar in sequence and function to those found in other organisms, studies performed in yeast can help us to determine how a particular gene or protein functions in higher eukaryotes (including humans). (jove.com)
  • Gene editing techniques exploit the HDR to modify the DNA to the desired sequence. (europa.eu)
  • For use as a comparator with a previously published 865 Mb genome based on the LD652 pupal ovary-derived cell line, as well as whole-insect genome sequences obtained from the Asian gypsy moth subspecies L. dispar asiatica and L. dispar japonica, the whole-insect LDD genome was sequenced, assembled and annotated. (usda.gov)
  • We report here the whole-genome sequences of the human-infective Trichuris trichiura and the mouse laboratory model Trichuris muris . (nature.com)
  • Here we present high-quality genome sequences for T. trichiura and T. muris , the first duo of a major human STH and its mouse counterpart. (nature.com)
  • There is the T5 promoter/lac operator transcription-translation system for expression in E. coli, the p10 promoter for baculovirus-based expression in insect cells, and the CAG (CMV/actin/globin) promoter for expression in mammalian cells. (qiagen.com)
  • All pacifastin members that have been characterized at the molecular level are precursor peptides composed of an N-terminal signal sequence followed by a precursor domain and a variable number of inhibitor domains. (wikipedia.org)
  • Isolation and structural determination of three peptides from the insect Locusta migratoria. (wikipedia.org)
  • This is the first report that establishes the presence of ALPs in the neuroendocrine tissues of insects and further studies are necessary to reveal common functions of these peptides, e.g. as antimicrobial agents, ion channel modulators or classical neuropeptides. (uni-koeln.de)
  • The described presence of agatoxin-like peptides in the nervous system of the honey bee and other insects was overseen so far and is thus a remarkable addition to the very well studied neuropeptidome of insects. (uni-koeln.de)
  • Therefore, methods for extracting the fly hemolymph efficiently and in sufficient quantities are essential for isolating and characterizing immune proteins and peptides. (bvsalud.org)
  • As they report in PLOS Neglected Tropical Diseases , the researchers used RNA sequencing and mass spectrometry to profile transcripts and proteins in saliva samples from nymph and adult kissing bugs raised at a Brazilian insectarium. (genomeweb.com)
  • Developing the project will boost my career as new skills in structural biology, including protein electron microscopy, will complement my previous expertise in protein-protein interaction and crosslinking mass spectrometry studies. (europa.eu)
  • For baculovirus-mediated expression in insect cells, we require expression vectors compatible with the Bac-to-Bac system (for example pFastBac, MultiBac, biGBac or MacroBac vectors). (embl.org)
  • QIA express pQE vectors combine a powerful phage T5 promoter (recognized by E. coli RNA polymerase) with a double lac operator repression module to provide tightly regulated, high-level expression of recombinant proteins in E. coli . (qiagen.com)
  • The pQE vectors (see table and figure pQE Vectors. Numbered elements listed in table. "> pQE Vectors ) enable placement of the 6xHis tag at either the N- or C-terminus of the recombinant protein. (qiagen.com)
  • The infective forms of T cruzi are contained in the feces of the insect vectors and gain entry into its mammalian hosts through contamination. (medscape.com)
  • Epimastigotes are an extracellular and noninfective form of the parasite found in the midgut of insect vectors, where they multiply by binary fission. (medscape.com)
  • Very little is known about the endogenous function of pacifastin-like inhibitors except that they may play roles in arthropod immunity and in regulation of the physiological processes involved in insect reproduction. (wikipedia.org)
  • They also contribute substantially to understanding the molecular basis of nematode pathogenicity and insect anti-nematode immunity. (bvsalud.org)
  • The Plasmodium falciparum reticulocyte-binding protein homolog 5 (PfRH5) has recently emerged as a leading candidate antigen against the blood-stage human malaria parasite. (nature.com)
  • We find that the fission yeast homologues of Tristetraprolin/TTP and Pumilio/Puf (Zfs1 and Puf3) interact with Ccr4-Not via multiple regions within low-complexity sequences, suggestive of a multipartite interface that extends beyond previously defined interactions. (elifesciences.org)
  • Currently we do not offer protein expression in other host organisms, but we can purify proteins expressed in other host organisms (e.g. yeast, hybridoma cell lines, …) if you are able to provide us with the expression cell pellet (intracellular proteins) or with the cell culture supernatant (secreted proteins). (embl.org)
  • It's also a protein sub-unit vaccine, but, instead of baculoviruses and moth cells, the manufacturing process uses yeast, similar to the methods that have been used for decades for Hepatitis B vaccines. (livemint.com)
  • Interestingly, many proteins found in yeast share similar sequences with proteins from their fellow Eukaryotes. (jove.com)
  • By investigating the function of a given protein in yeast, researchers gain insight into the protein's function in higher eukaryotes, such as us, humans. (jove.com)
  • An expressed sequence tag (EST) library was constructed from mRNA obtained from fat bodies of A. mylitta larvae that had been challenged by infection with Escherichia coli cells. (biomedcentral.com)
  • We offer tools for genomics analysis, protein analysis, and antibody screening techniques to provide a comprehensive approach to antigen discovery. (sigmaaldrich.com)
  • Results indicated that the aquaporin protein was effective as an antigen in cattle vaccines to help prevent cattle fever tick infestations. (feedstuffs.com)
  • The assay, which utilizes a truncated 56 kDa recombinant HEV capsid protein expressed in insect cells as antigen, was performed as described previously (1), with modifications (2). (cdc.gov)
  • However, flow cytometry data for whole-insect LDD indicates it has a genome size more than 10% larger than its cell line counterpart. (usda.gov)
  • Insect OS-D-like proteins, also known as chemosensory (CSP) or sensory appendage proteins (SAP), are broadly expressed in various insect tissues, where they are thought to bind short to medium chain length fatty acids and their derivatives. (gc.ca)
  • Chemosensory proteins (CSPs) are a class of small (10C15 kDa) soluble proteins comprising 4 conserved cysteines which abundantly exist in the chemoreceptive organs and transmit chemical signals to nervous system [7]C[8]. (woofahs.com)
  • Next-generation sequencing confirmed the vector backbone was not incorporated into the plant and the presence of a single T-DNA integration site. (cbd.int)
  • The pQE TriSystem Vector allows for high-level expression of His-tagged proteins from a single vector containing three different expression systems. (qiagen.com)
  • Our results reveal an important role played by the melanization pathway in response to ZIKV infection, hence highlighting the importance of this pathway in insect host defense against viral pathogens and potential vector control strategies to alleviate ZIKV outbreaks. (bvsalud.org)
  • The epimastigote form of Trypanosoma cruzi is the multiplying stage of the parasite that grows in the gut of the insect vector and also in cell-free culture medium as shown here. (medscape.com)
  • Characterization (polymerase chain reaction, nucleic acid sequencing) and mutagenisis of cloned DNA fragments. (up.ac.za)
  • Functional and structural characterization of human adrenoleukodystrophy protein (ALDP). (elifesciences.org)
  • Sequencing of the whole-insect LDD genomes was also motivated by a desire to identify the genetic components associated with flight in Asian gypsy moth females, a characteristic which is absent in European gypsy moth females. (usda.gov)
  • A neighbor joining distance phenogram identified several protein similarity classes that were characterized by highly conserved sequence motifs, including (A) N-terminal YTTKYDN(V/I)(N/D)(L/V)DEIL, (B) central DGKELKXX(I/L)PDAL, and (C) C-terminal KYDP. (gc.ca)
  • The full genome was sequenced, and closest similarity was found to EILV. (microbiologyresearch.org)
  • Emily Anthes braves locusts, beetles, mealworms and more as she asks whether eating insects is the answer to feeding ever more humans and livestock. (newstatesman.com)
  • Infected insects take blood meals from humans and their domestic animals and deposit parasite-laden feces. (medscape.com)
  • The more mRNA copies it makes, the more protein it can produce. (elifesciences.org)
  • A simple way to control protein production is to raise or lower the number of these mRNA messages, and living cells have lots of ways to make this happen. (elifesciences.org)
  • For in vitro delivery of mRNA for applications such as stem cell induction, protein replacement, cancer immunotherapies, or vaccine modalities, synthetic mRNA has become a critical tool in diverse biopharmaceutical approaches. (sigmaaldrich.com)
  • Cotton ( Gossypium hirsutum ) modified to express the modified crystal protein mCry51Aa2 from Bacillus thuringiensis strain EG2934 to confer protection against Hemipteran and Thysanopteran insect pests, particularly tarnished plant bugs ( Lygus hesperus and Lygus lineolaris ), cotton fleahopper ( Pseudoalomoscelis seriatus ) and thrips ( Frankiniella spp. (cbd.int)
  • This modified protein contains eight amino acid substitutions(F46S, Y54H, S95A, F147S, Q149E, S167R, P219R, R273W) and a deletion of three amino acids (Δ196-198), which improve the effectiveness of the protein against the major Hemipteran cotton pests Lygus hesperus and Lygus lineolaris . (cbd.int)
  • This information will be used by scientific researchers and stakeholders or parties interested in controlling these invasive insect pests. (usda.gov)
  • The triggers differ from quitoes, storage pests, biting insects, canine and feline person to person, most likely due to a combination of fleas, butterfly larvae, bedbugs, horseflies, silverfish and environmental and genetic factors. (who.int)
  • ImmunoCAP positive sera to soy (n=16) were tested in IgE immunoblots and antigens were sequenced. (cdc.gov)
  • Most use lysates of epimastigotes as target antigens, but several are based on recombinant proteins. (medscape.com)
  • Preliminary studies can be carried out in bacterial expression systems, using the strong T5 promoter, which is recognized by E. coli polymerase, and allows efficient expression of proteins in any E. coli strain. (qiagen.com)
  • Inserts encoding proteins of interest are cloned into appropriate constructs and transformed into a suitable E. coli strain for expression. (qiagen.com)
  • Silks are composed principally of proteins with a predominance of alanine, serine and glycine and silk proteins are able to undergo irreversible transformations from soluble protein to insoluble fibres. (bioone.org)
  • However it has proved challenging to identify a heterologous expression platform that can produce a soluble protein-based vaccine in a manner compliant with current Good Manufacturing Practice (cGMP). (nature.com)
  • This work identifies Drosophila S2 cells as a clinically-relevant platform suited for the production of 'difficult-to-make' proteins from Plasmodium parasites, and identifies a PfRH5 sequence variant that can be used for clinical production of a non-glycosylated, soluble full-length protein vaccine immunogen. (nature.com)
  • Water-soluble proteins of royal jelly were fractionated and investigated for the proliferative and migratory effects on human epidermal keratinocytes (HaCaT) in an in vitro wound healing model. (biomedcentral.com)
  • We fractionated the water-soluble proteins of royal jelly and identified one fraction (Fraction 2) that induced both proliferative and migratory effects on a human epidermal keratinocyte cell line. (biomedcentral.com)
  • These proteins are often homologous, and their similar sequences indicate that the organisms share a common ancestor. (jove.com)
  • Insects are evolutionarily successful organisms and occupy almost all habitats in nature. (biomedcentral.com)
  • Several biological parameters of this insect were assessed under laboratory conditions (25 ± 2° C, 75 ± 5% relative humidity, and a photoperiod of 16:8 [L:D] h). (bioone.org)
  • In xenodiagnosis, 30-40 laboratory-reared insects are allowed to feed directly or indirectly on the blood of a person suspected to have Chagas disease. (medscape.com)
  • Plasmids purified from genomic DNA, proteins, ribosomes, and the bacterial cell wall are used in molecular biology research. (sigmaaldrich.com)
  • Predicted to enable DNA-binding transcription repressor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. (nih.gov)
  • These regulatory modules include promoters, insulators, silencers, enhancers, promoter targeting sequences and the recently identified promoter tethering element (PTE). (nih.gov)
  • Transcription of mCry51Aa2 coding sequence directed by the Arabidopsis thaliana heat shock protein 81-2 promoter (P- hsp81-2) , with enhanced activity due to the Figwort mosaic virus 35S enhancer (E-FMV) located adjacent to the promoter. (cbd.int)
  • Immunoblotting of leaf samples and enzyme-linked immunosorbent assay (ELISA) of leaf, pollen, root, and seed samples confirmed the expression of the protein. (cbd.int)
  • Using a two-color assay to simultaneously monitor poly(A) tail removal from different RNAs, we demonstrate that Puf3 can distinguish between RNAs of very similar sequence. (elifesciences.org)
  • A trypsin inhibitor assay reveals that Ara h 3 allergen protein has trypsin inhibitory activity. (usda.gov)
  • Known as "destabilising motifs", these sequences attract the attention of a group of proteins called Ccr4-Not. (elifesciences.org)
  • Spiders like a few other arachnids as well as many insects produce silk. (bioone.org)
  • For example, silk fibroin, a class of proteins produced by many insects and arachnids, is an archetypal elastomer with an unrivaled combination of strength and toughness. (techconnectworld.com)
  • RNA-binding proteins contribute to specificity by interacting with both Ccr4-Not and target mRNAs, but this is not fully understood. (elifesciences.org)
  • Its substrate specificity suggests that Tsp may contain a substrate recognition domain, which selectively binds to the nonpolar C-termini of substrate proteins, separate from its catalytic site. (embl.de)
  • Detection of the effector protein allows the potato plant to initiate its native immune response to resist infection by P . infestans . (canada.ca)
  • Furthermore, GSOs protected cells against GLU-induced apoptosis by reducing the expression of the mitochondrial apoptosis-associated Bcl-2 family effector proteins and protected cells from GLU-induced oxidative damage by increasing the nuclear translocation of Nrf2 and HO-1 expression. (sdbonline.org)
  • The proteins are subsequently secreted into the glandular lumen where they are stored until extrusion. (bioone.org)
  • Redzepi, who founded the Nordic Food Lab in 2008, became interested in serving insects at Noma and asked the researchers at the lab to explore the possibilities. (newstatesman.com)
  • The constituent proteins are normally synthesised in specialised glands where the epithelial cells are responsible for the biosynthesis. (bioone.org)
  • The first has been developed by Maryland-based Novavax Inc. It encodes genetic sequences from the spike protein into a virus that targets insects, then loads that virus into cultured moth cells. (livemint.com)
  • Human PRB1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade. (antibodies-online.com)
  • If expression in mammalian or insect cells is required - to obtain post-translational modifications, for example - the same construct can be used without the need for time-consuming subcloning procedures. (qiagen.com)
  • On the basis of high-throughput transcriptomics data, we identify whipworm proteins that are highly expressed in the anterior region of the parasite, that is, in intimate contact with the cytoplasm of host intestinal cells and the immune system. (nature.com)
  • A protein fraction, mainly containing major royal jelly proteins 2 (MRJP2), MRJP3 and MRJP7, stimulated proliferative and migratory activities in HaCaT cells without visible cytotoxicity. (biomedcentral.com)
  • China is already planting groves of genetically modified poplars that have been made to be resistant to insects. (bioedonline.org)
  • Reactivity towards CP4-EPSPS, a protein found in genetically-modified soy processed at the plant, was investigated. (cdc.gov)
  • What is the cellular localization of the protein? (embl.org)
  • Based on the structure, we performed integrative analysis of the cellular trafficking, protein thermostability, ATP hydrolysis, and the transport activity of representative mutations. (elifesciences.org)
  • The insect immune response is comprised of cellular and humoral components. (biomedcentral.com)
  • Using RNA sequencing data from whipworm-infected mice, we describe the regulated T helper 1 (T H 1)-like immune response of the chronically infected cecum in unprecedented detail. (nature.com)
  • Does the protein require interaction partners for stability (→ co-expression)? (embl.org)
  • After we receive the sequence-verified expression constructs, we will first generate the recombinant baculoviruses. (embl.org)
  • For protein expression we use either the Sf21 or the Hi5 insect cell lines. (embl.org)
  • As mentioned above, the late blight resistance trait is achieved through the expression of the VNT1 protein. (canada.ca)
  • The petitioner has provided information describing the methods used to develop Simplot Innate® potato events Gen2-W8, Gen2-X17, and Gen2-Y9 and the molecular biology data that characterize the genetic change, which results in reduced levels of free asparagine in tubers, lower levels of reducing sugars (i.e., glucose and fructose) in tubers, reduced expression of polyphenol oxidase 5 enzyme (Ppo5), and expression of the VNT1 protein. (canada.ca)
  • The antibodies induced by one protein variant were shown to be qualitatively similar to responses induced by other vaccine platforms. (nature.com)
  • While sequencing the cattle tick's genome, insect physiologist Felix D. Guerrero and his colleagues at the ARS Tick & Biting Fly Research Unit in Kerrville, Texas, identified several proteins that, when formulated as a cattle vaccine, could potentially kill cattle ticks. (feedstuffs.com)
  • One of the proteins, aquaporin, was developed into a recombinant tick aquaporin protein vaccine. (feedstuffs.com)
  • The ARS-patented aquaporin protein vaccine provides an alternative to chemicals to reduce the risk of tick infestation, ARS said, noting that it is exploring the possibility of producing a commercial aquaporin vaccine with a private company. (feedstuffs.com)
  • The DoE announced in January plans to sequence the soybean (Glycine max), a major source of biodiesel. (bioedonline.org)
  • The mRNAs can carry short sequences of genetic letters that can trigger their own destruction. (elifesciences.org)
  • Applications in protein engineering, diagnostics and synthesis of useful products. (up.ac.za)
  • Protein synthesis is effectively blocked in the presence of high levels of lac repressor and the stability of cytotoxic constructs is enhanced. (qiagen.com)
  • Along with mushrooms and molds, S. cerevisiae belongs to the Kingdom Fungi due to the presence of a cell wall made out of chitin, a polysaccharide polymer that's found not only in Fungi, but also in the exoskeletons of insects and crustaceans. (jove.com)
  • Other segments include fracking wastewater accumulation found in freshwater mussels' shells and a new protein sequencing method used in biological research. (dcmp.org)
  • PDZ domains can occur in one or multiple copies and are nearly always found in cytoplasmic proteins. (embl.de)
  • PDZ domain proteins are frequently associated with the plasma membrane, a compartment where high concentrations of phosphatidylinositol 4,5-bisphosphate (PIP2) are found. (embl.de)
  • [ 1 ] T cruzi mostly is found in blood-sucking triatomine insects (kissing bugs) and small mammals in a sylvatic cycle that is enzootic from the southern and southwestern United States to central Argentina and Chile. (medscape.com)
  • Two alternatively spliced transcript variants encoding the same protein have been described for MAP4K5. (thermofisher.com)
  • As with other parasites that infect both mammalian and insect hosts, the life cycle of T cruzi is complex (see image below). (medscape.com)
  • At least one month later, intestinal contents of the insects are extracted and examined microscopically for the presence of parasites. (medscape.com)
  • A genome sequence was recently reported for a gypsy moth cell line, which was derived from LDD ovary tissues over 40 years ago. (usda.gov)
  • Sequence analysis of two of these clones revealed a 96% and 93% similiarity with peanut allergens Ara h 4 and Ara h 3, respectively. (usda.gov)
  • Background: Peanut allergens and peanut trypsin inhibitors are seed storage proteins. (usda.gov)
  • The proteins present in bioactive fractions were characterised and quantified using Label-free protein quantification method. (biomedcentral.com)
  • SCOP: Structural Classification of Proteins and ASTRAL. (berkeley.edu)
  • SCOPe: Structural Classification of Proteins - extended. (berkeley.edu)
  • 2.2 Cloning and Sequence Analysis of CSPSlit Cloning and sequence analysis of NlFoxA Total RNA was isolated from four 2 nd day time brachypterous female adults of N. lugens using the Trizol kit (Invitrogen, USA). (woofahs.com)
  • Different groups are looking at sequencing the eucalyptus and peach trees among others. (bioedonline.org)