• Iron-sulfur cluster assembly 1 homolog, mitochondrial is an evolutionarily highly conserved protein for the biogenesis of iron-sulfur cluster across species. (wikipedia.org)
  • To identify determinants of mETC function, we screened a genome-wide human CRISPRi library under oxidative metabolic conditions with selective inhibition of mitochondrial Complex III and identified OCIA domain-containing protein 1 (OCIAD1) as a Complex III assembly factor. (biorxiv.org)
  • We find that OCIAD1 is an inner mitochondrial membrane protein that forms a complex with supramolecular prohibitin assemblies. (biorxiv.org)
  • As a test case, we reconstruct the topology of the reaction and regulatory network for the mitochondrial ISC biogenesis pathway in S. cerevisiae . (biomedcentral.com)
  • A putative role for frataxin in directly regulating mitochondrial iron import is discarded from our analysis, which agrees with also published experimental results. (biomedcentral.com)
  • Here, we review mechanisms of mitochondrial gene expression with a focus on the recent findings in the field of mammalian mtDNA transcription and disease phenotypes caused by defects in proteins involved in this process. (biomed.news)
  • One such class of pathways is involved in the biogenesis of Iron-Sulfur Clusters (ISC). (biomedcentral.com)
  • Involved in the biogenesis and/or repair of oxidatively damaged Fe S clusters. (or.jp)
  • As mono or diiron center as well as incorporated into heme or iron-sulfur clusters, this metal is an indispensable cofactor for a variety of cellular processes including electron transport, amino acid metabolism, and biosynthesis of DNA and sterols. (frontiersin.org)
  • Iron-sulfur (Fe-S) clusters are essential cofactors present in all known forms of life and required by hundreds of proteins to perform their function. (curefa.org)
  • CF27-specific gene clusters which have been analyzed were expressed by both ferrous iron-grown and sulfur-attached cells, indicating that they are not pseudogenes and may play a role in both situations. (frontiersin.org)
  • After focusing for some time on the capacity of several plant GRXs to assemble \[2Fe-2S\] centers into homodimers or into heterodimers with BOLA proteins, we extended our research interest to the functional characterization of the associated protein families (NFUs, SUFA/ISCAs, IBA57s, HCF101/INDH) assumed to participate in the transfer of these Fe-S clusters together with GRX and BOLA. (hal.science)
  • This functional analysis will combine plant genetics and physiology, molecular and structural biology and biochemistry approaches to determine whether these proteins assemble different types of Fe-S clusters and have specific interaction partners and what are the physiological and metabolic consequences of deleting these genes for plant development and physiology. (hal.science)
  • Increasing amounts of data that can be mined for information about how proteins in cells assemble as metabolic pathways, signal transduction pathways, and gene circuits, are generated each day. (biomedcentral.com)
  • Iron-Sulfur Cluster biogenesis) or complex pathways with an unclear reaction and regulation network, (e. g. cell cycle). (biomedcentral.com)
  • During iron starvation, HapX represses iron-consuming pathways, including heme biosynthesis and respiration, to spare iron and activates synthesis of ribotoxin AspF1 and siderophores, the latter partly by ensuring supply of the precursor, ornithine. (frontiersin.org)
  • We hope to unravel new redox-regulated cellular processes or signaling pathways in poplar and more generally in plants which are controlled by redox reactions such as thiol-disulfide exchanges and understand how the functions of these proteins are controlled at the cellular level by TRX and GRX. (hal.science)
  • In this context, the intervention of the TRX and glutathione (GSH)/GRX reducing systems in the sulfur signaling/trafficking pathways is examined as both the TRX and GSH/GRX systems might modulate STR function and in particular be central to the formation of hydrogen sulfide, a molecule susceptible to trigger redox signalling cascades in several physiological situations. (hal.science)
  • Using the Iron Sulfur cluster biogenesis in S. cerevisiae as a test case we indicate how this procedure can be used to analyze and validate the network model against experimental results. (biomedcentral.com)
  • Yersinia genomes had a similar global partition of protein functions, as measured by the distribution of Cluster of Orthologous Groups families. (biomedcentral.com)
  • Genomic sequencing of A. fulgidus suggests that three [Ni-Fe]-CODHs are present, a "bacterial" monomeric CooS and two archaeal CdhAB-type CODHs [ 9 - 11 ]. (hindawi.com)
  • Most of the remaining genes were in regions of genomic mobility and were strain-specific or conserved in other Geobacteraceae , indicating that the changes occurred post-divergence. (biomedcentral.com)
  • New DNA sequencing technologies have enabled detailed comparative genomic analyses of entire genera of bacterial pathogens. (biomedcentral.com)
  • To further investigate the genomic diversity among this group and to help characterize lineages of the plague organism that have no sequenced members, we present here the genomes of two isolates of the "classical" antiqua biovar, strains Antiqua and Nepal516. (asm.org)
  • The genomic component of the CRISPR-Cas system is formed by a series of variable spacers, which in some cases share sequence similarity with viruses, plasmids, or bacteria. (pjmonline.org)
  • Datasets available for such tasks include the primary literature, large scale micro array experiments, whole genome two hybrid screenings, full genome sequences, and the patterns of conserved/non-conserved homologues and orthologues in them. (biomedcentral.com)
  • Thus, it is now thought that all eukaryotes contain an organelle related to mitochondria, and its most conserved function is in Fe/S protein biogenesis, not ATP production. (ncl.ac.uk)
  • strain CF27, new sequences were generated, and an update assembly and functional annotation were undertaken, followed by a comparative analysis with other Acidithiobacillus species whose genomes are publically available. (frontiersin.org)
  • Here we describe the deployment of new sequencing technology to extensively sample eight genomes from the Yersinia genus of the family Enterobacteriaceae. (biomedcentral.com)
  • We compare all five currently sequenced Y. pestis genomes and the corresponding features in Yersinia pseudotuberculosis . (asm.org)
  • The geno-mic component is a DNA loci containing short frag-ments of targeted nucleic acid sequences (spacers) inter-spaced by short repeated sequences (repeats). (pjmonline.org)
  • The identification and functional characterization of 24 genes that are involved in iron homeostasis in A. fumigatus and/or Aspergillus nidulans , respectively, revealed significant insights into iron metabolism and its regulation (Table 1 ). (frontiersin.org)
  • Mai, H., Baby, D., & Bauer, P. (2023) Black sheep, dark horses and colorful dogs: A review on the current state of the Gene Ontology with respect to iron homeostasis in Arabidopsis thaliana . (hhu.de)
  • The C-terminal tail of ribosomal protein Rps15 is engaged in cytoplasmic pre-40S maturation. (db-engine.de)
  • Nevertheless, iron excess has the potential to catalyze the formation of cell-damaging reactive oxygen species ( Halliwell and Gutteridge, 1984 ). (frontiersin.org)
  • has been shown to consist of at least seven species, all of which derive energy from the oxidation of elemental sulfur and reduced inorganic sulfur compounds (RISCs) to support their growth. (frontiersin.org)
  • Prior to this study, three species of the enterobacterial genus Yersinia that cause invasive human diseases ( Yersinia pestis , Yersinia pseudotuberculosis , and Yersinia enterocolitica ) had been sequenced. (biomedcentral.com)
  • protein system, it would be very unlikely that all modern species would have the same code. (whyevolutionistrue.com)
  • the next seven years showing that the mitosome functions in the biosynthesis of essential cytosolic and nuclear Iron/Sulphur (Fe/S) proteins . (ncl.ac.uk)
  • Recently, the sterol regulatory element binding protein SrbA was found to be essential for adaptation to iron starvation, thereby linking regulation of iron metabolism, ergosterol biosynthesis, azole drug resistance, and hypoxia adaptation. (frontiersin.org)
  • Environmental sequences of ten novel plastid lineages and structural innovations in plastid proteins confirm that plastids in apicomplexans and their relatives are widespread and share a common, photosynthetic origin. (elifesciences.org)
  • These repeats can drive at least three consistent unliganded containing proteins processing on which organizations they are. (evakoch.com)
  • The clustered regularly interspaced palindromic repeats-CRISPR-associated proteins (CRISPR-Cas) system provides the majority of bacteria and archaea with adaptive and hereditary immunity against this threat. (pjmonline.org)
  • 2013). However, a unique defense system involving clustered regularly interspaced palindromic repeats-CRISPR-associated proteins (CRISPR-Cas) was reported recently. (pjmonline.org)
  • For our research projects, we employ biochemical, biophysical, spectroscopic and structural biology approaches with *Populus trichocarpa* and *Arabidopsis thaliana* as model organisms to explore (i) the roles of the antioxidant/detoxification systems in the physiology of plants subject to environmental constraints and (ii) the maturation and roles of iron-sulfur proteins in plant organelles. (hal.science)
  • Erko had worked with Carl Woese in pioneering the use of ribosomal RNA sequences to investigate evolutionary relationships among prokaryotes. (ncl.ac.uk)
  • The Museum wanted to set up a lab using molecular sequences to investigate evolutionary relationships. (ncl.ac.uk)
  • We found 453 single nucleotide polymorphisms in protein-coding regions, which were used to assess the evolutionary relationships of these Y. pestis strains. (asm.org)
  • Rapid high-quality draft sequencing was used successfully to compare pathogenic and non-pathogenic members of the Yersinia genus. (biomedcentral.com)
  • Multiple membrane-bound heterodisulfide reductase (DsrMK) could promote both energy-conserving and non-energy-conserving menaquinol oxidation. (hindawi.com)
  • Enoyl-(Acyl carrier protein) reductase [Interproscan]. (ntu.edu.sg)
  • Despite its high abundance in the Earth's crust, the bioavailability of iron is low owing to its oxidation into sparingly soluble ferric (Fe 3+ ) hydroxides by atmospheric oxygen. (frontiersin.org)
  • In Aspergillus fumigatus , iron starvation causes extensive transcriptional remodeling involving two central transcription factors, which are interconnected in a negative transcriptional feed-back loop: the GATA-factor SreA and the bZip-factor HapX. (frontiersin.org)
  • Accepted August 23, 2012 ABSTRACT The catabolite control protein CcpA is a pleiotropic regulator that mediates the global transcriptional response to rapidly catabolizable carbohydrates, like glucose in Gram-positive bacteria. (studyres.com)
  • This occurs through the downregulation of methylmalonyl coenzyme A epimerase (MCEE), mediated by an extracellular signal-regulated kinase 2-driven transcription factor Sp1/early growth response protein 1 transcriptional switch driven by metastatic signalling at its promoter level. (biomed.news)
  • miRNAs are either expressed from independent transcriptional units or derive from introns of protein-coding genes or exons or introns of long ncRNAs. (biomedcentral.com)
  • This activates signaling to the actomyosin cortex via nuclear envelope stretch-sensitive proteins, up-regulating cell contractility. (regenerativemedicine.net)
  • Protein of unknown function (DUF520) [Interproscan]. (ntu.edu.sg)
  • I am a biochemist interested in basic plant science processes with a particular research focus on structure-function analysis of oxidoreductases and on redox post-translational modifications of proteins. (hal.science)
  • Redox post-translational modifications of proteins** In addition to performing structure-function analyses of thioredoxin (TRX) and glutaredoxin (GRX) family members which control most of the reversible oxidative modifications of protein cysteinyl residues, we are characterizing proteins of unknown function possessing one or several conserved CXXC motifs known to be particularly suited for disulfide bond formation but also other redox modifications. (hal.science)
  • MFSD1 is a lysosomal membrane-bound solute carrier protein with no previously described function in immunity. (x-mol.com)
  • 25% of orthologs had sequence differences, and this set was enriched in phosphorylation and ATP-dependent enzymes. (biomedcentral.com)
  • protein_coding" "AAC74185","ptsG","Escherichia coli","fused glucose-specific PTS enzymes: IIB component/IIC component [Ensembl]. (ntu.edu.sg)
  • The same questions and strategies apply to sulfurtransferases, enzymes that catalyze the transfer of a sulfur atom from sulfur donors to nucleophilic sulfur acceptors (trans-persulfidation) by forming themselves persulfides. (hal.science)
  • In this review, we will discuss the functional roles of RNA binding proteins and their lncRNA partners in TCA cycle, with emphasis placed on the cancer progression. (bvsalud.org)
  • The complex intertwining of iron metabolism and oxidative stress is emphasized by the iron-dependence of detoxification of oxidative stress as, e.g., catalases and peroxidases require heme as cofactor. (frontiersin.org)
  • We generated single-cell transcriptomes for all major apicomplexan groups lacking large-scale sequence data. (elifesciences.org)
  • events have receptors stimulated with students( GAGs), unregulated chains docking of a inactive actin, all of an been protein respiration cell a considerable subunits. (evakoch.com)
  • Cysteine, a thiol-containing amino acid, is crucial for the synthesis of sulfur-containing biomolecules that control multiple essential cellular activities. (biomed.news)
  • A further understanding of RNA binding proteins and their lncRNA partners in TCA cycle, as well as their molecular mechanisms in oncogenesis, will aid in developing novel layers of metabolic targets for cancer therapy in the near future.Abbreviations: CS: citrate synthase. (bvsalud.org)
  • The achievement, at a global level, of a "healthy aging phenotype" defined as "the condition of being alive, while having highly preserved functioning metabolic, hormonal and neuroendocrine control systems at the organ, tissue and molecular levels" [ 10 ], is the most ambitious objective that modern science should achieve. (iospress.com)
  • The miRNA duplexes are loaded into an Argonaute protein in the miRNA-induced silencing complex (miRISC) and rapidly unwound. (biomedcentral.com)
  • Pre-miRNAs are exported to the cytoplasm by Exportin-5 and processed further by Dicer, to ~22 nt double-stranded miRNA duplexes that are loaded into an Argonaute protein in the miRISC and rapidly unwound. (biomedcentral.com)
  • Encodes a ClpP-related sequence. (or.jp)
  • This review summarizes the current knowledge on iron homeostasis and its role in virulence in Aspergillus spp. (frontiersin.org)
  • Review: Role of the plant-specific calcium-binding C2-DOMAIN ABSCISIC ACID-RELATED (CAR) protein family in environmental signaling. (hhu.de)
  • Transformation of Chaetomium thermophilum and affinity-purification of native thermostable protein complexes. (db-engine.de)
  • Aftertranscription, these fragments guide protein complexes that target foreign nucleic acids and promote their degradation. (pjmonline.org)
  • Lichtblau DM , Schwarz B , Baby D , Endres C, Sieberg C and Bauer P (2022) The Iron Deficiency-Regulated Small Protein Effector FEP3/IRON MAN1 Modulates Interaction of BRUTUS-LIKE1 With bHLH Subgroup IVc and POPEYE Transcription Factors. (hhu.de)
  • Plague is a zoonotic disease, endemic throughout the world, and highly infectious in humans. (asm.org)
  • Most genetic diseases in humans are caused by point mutations-single base errors in the DNA sequence. (pharmaceuticalintelligence.com)
  • Iron is an essential nutrient for all eukaryotes and nearly all prokaryotes ( Kaplan and Kaplan, 2009 ). (frontiersin.org)
  • Aldehyde dehydrogenase family, Iron-containing alcohol dehydrogenase [Interproscan]. (ntu.edu.sg)
  • Predictions regarding how proteins act in ISC biogenesis are validated by comparison with published experimental results. (biomedcentral.com)
  • Iron is an essential but, in excess, toxic nutrient. (frontiersin.org)
  • Transcription of mtDNA is not only essential for the biogenesis of the OXPHOS system, but also generates RNA primers necessary to initiate mtDNA replication. (biomed.news)
  • Deficiency in HapX, but not SreA, attenuates virulence of A. fumigatus in a murine model of aspergillosis, which underlines the crucial role of adaptation to iron limitation in virulence. (frontiersin.org)
  • All of the virulence-associated genes are transcriptionally upregulated during iron starvation and encode functions that are important for survival during iron starvation, which emphasizes the crucial role of adaptation to iron starvation in virulence. (frontiersin.org)
  • To achieve such a goal, strategies that combine the different theoretical and computational methods to identify proteins and generate a set of plausible alternative network topologies for the process of interest are needed. (biomedcentral.com)
  • protein_coding" "AAC74888","manY","Escherichia coli","mannose-specific enzyme IIC component of PTS [Ensembl]. (ntu.edu.sg)
  • Researchers supply cells with a template containing the desired sequence, make a targeted double-strand break with the Cas9 enzyme, and then wait to see whether homology-directed repair incorporates the template to reconnect the strands. (pharmaceuticalintelligence.com)
  • These regions are interspaced with repeat sequences that are identical or almost identical within a single CRISPR cassette. (pjmonline.org)
  • Using the technique at six loci in human cells, the team reported a targeted base correction rate of up to 37 percent, with only around 1 percent of the sequences showing indels. (pharmaceuticalintelligence.com)
  • The control over access to iron is one of the central battlefields during infection as pathogens have to "steal" the iron from the host. (frontiersin.org)