• Genes that confer resistance to antimicrobial drugs used in human or veterinary medicine are often mobilized on plasmids. (cdc.gov)
  • The ability and frequency with which antimicrobial resistance genes disseminate between bacteria in humans, the environment, and animals is still debated, and the role of plasmids in this movement between ecosystems, including the food chain, is also still contested, despite mounting evidence that it occurs ( 8,9 ). (cdc.gov)
  • Replication genes were not identified in some plasmids, a situation that has led to the possibility of host interaction involvement. (frontiersin.org)
  • Some plasmids were also void of par genes and intriguingly, repA gene was also not discovered in these plasmids. (frontiersin.org)
  • Plasmid stabilization/stability protein-encoding genes were observed in some plasmids but were not established for participating in plasmid segregation. (frontiersin.org)
  • Conjugation genes were identified portraying the conjugation ability amongst Pandoraea plasmids. (frontiersin.org)
  • Additionally, we found a shared region amongst some of the plasmids that consists of conjugation genes. (frontiersin.org)
  • The identification of genes involved in replication, segregation, toxin-antitoxin systems and conjugation, would aid the design of drugs to prevent the survival or transmission of plasmids carrying pathogenic properties. (frontiersin.org)
  • Additionally, genes conferring virulence and antibiotic resistance were identified amongst the plasmids. (frontiersin.org)
  • In an Aug. 8, 2019, patent filed by Moderna regarding RNA vaccines, the company states that the direct injection of naked plasmid DNA into a living host comes with "potential problems, including the possibility of insertional mutagenesis, which could lead to the activation of oncogenes or the inhibition of tumor suppressor genes. (zerohedge.com)
  • Some of the most clinically relevant resistance genes, such those encoding carbapenemases (ß-lactamase enzymes able to degrade carbapenem antibiotics), are carried on conjugative plasmids that spread across high-risk bacterial clones 5 , 6 . (nature.com)
  • AMR, through acquired genes located on transposons or conjugative plasmids, is the horizontal transmission of genes required for a given bacteria to withstand antibiotics. (mdpi.com)
  • In nature, plasmids often carry genes that benefit the survival of the organism and confer selective advantage such as antibiotic resistance. (wikipedia.org)
  • While chromosomes are large and contain all the essential genetic information for living under normal conditions, plasmids are usually very small and contain only additional genes that may be useful in certain situations or conditions. (wikipedia.org)
  • Smaller plasmids make use of the host replicative enzymes to make copies of themselves, while larger plasmids may carry genes specific for the replication of those plasmids. (wikipedia.org)
  • Many of the genes carried by a plasmid are beneficial for the host cells, for example: enabling the host cell to survive in an environment that would otherwise be lethal or restrictive for growth. (wikipedia.org)
  • By contrast, plasmids are usually excluded or analyzed with low-resolution techniques, despite being the primary vectors of antibiotic resistance genes across many key pathogens. (ox.ac.uk)
  • First, bla OXA-48-like genes have spread primarily via the single epidemic pOXA-48-like plasmid, which emerged recently in clinical settings and spread rapidly to numerous lineages. (ox.ac.uk)
  • Second, bla VIM and bla NDM genes have spread via transient associations of many diverse plasmids with numerous lineages. (ox.ac.uk)
  • Third, bla KPC genes have transmitted predominantly by stable association with one successful clonal lineage (ST258/512) yet have been mobilized among diverse plasmids within this lineage. (ox.ac.uk)
  • Although a high degree of similarity exists between the two sequenced Pseudomonads, 976 protein-encoding genes are unique to Pss B728a when compared with Pst DC3000, including large genomic islands likely to contribute to virulence and host specificity. (nih.gov)
  • The E. coli was found to contain the mcr-1 gene on a transmissible plasmid as well as genes conferring other types of antibiotic resistance. (genomeweb.com)
  • But if the mcr-1 gene is transferred and ends up on a resistance plasmid that contained genes encoding carbapenemases or 16s methylation , for example, it could be dire. (genomeweb.com)
  • The E. coli in question wound up having two plasmids with different complements of other antibiotic resistance genes, including extended-spectrum beta-lactamase genes, but Snesrud emphasized that it was not a pan-resistant strain. (genomeweb.com)
  • blaOXA-163 was located on a 71-kb IncN plasmid with other resistance genes. (ox.ac.uk)
  • The presence of blaOXA-48-like genes on epidemic plasmids in ST131 is of concern. (ox.ac.uk)
  • Prediction of the coding sequences of unidentified human genes. (nih.gov)
  • Many plasmid-encoded genes could be involved in several functions other than toxin production. (bgu.ac.il)
  • The T3SS imposes a significant cost on the bacterium, and strains which have lost the plasmid and/or genes encoding the T3SS grow faster than wild-type strains in the laboratory, and fail to bind the indicator dye Congo Red (CR). (ox.ac.uk)
  • The whole-genome sequence of strain K-4, isolated from grass silage in Thailand, which constitutes a chromosome and two plasmids, is 2,914,933 bp long, has a GC content of 37.5%, and contains 2,734 predicted protein-coding genes. (bvsalud.org)
  • lactamase encoding genes ( bla ) including bla NDM-1 and plasmid-mediated quinolone and aminoglycoside resistance determinants. (who.int)
  • The virulence of this bacterium results from the 32 Y pestis chromosomal genes and two Y pestis -specific plasmids, constituting the only new genetic material acquired since its evolution from its predecessor. (medscape.com)
  • Bacterial plasmids are key vectors of horizontal gene transfer, mediating the mobilization of genetic material from 1 bacterium to another. (cdc.gov)
  • therefore, dissemination has been attributed to conjugative plasmids rather than to clonal expansion of a bacterial host strain ( 13 ). (cdc.gov)
  • Plasmid persistence in bacterial populations is strongly influenced by the fitness effects associated with plasmid carriage. (nature.com)
  • However, plasmid fitness effects in wild-type bacterial hosts remain largely unexplored. (nature.com)
  • Moreover, genomic results showed a link between pOXA-48_K8 fitness effects and bacterial phylogeny, helping to explain plasmid epidemiology. (nature.com)
  • Incorporating our fitness results into a simple population dynamics model revealed a new set of conditions for plasmid stability in bacterial communities, with plasmid persistence increasing with bacterial diversity and becoming less dependent on conjugation. (nature.com)
  • These fitness costs make it difficult to explain how plasmids are maintained in bacterial populations over the long-term in the absence of selection for plasmid-encoded traits, a puzzle known as "the plasmid-paradox" 10 . (nature.com)
  • These examples do not necessarily replicate plasmid fitness effects in natural bacterial hosts, which remain largely unexplored. (nature.com)
  • To fully understand plasmid persistence in natural bacterial populations, it will be necessary to address these limitations. (nature.com)
  • In this study, we provide a detailed characterisation of the distribution of plasmid fitness effects in wild-type bacterial hosts. (nature.com)
  • This plasmid contains a constitutive bacterial promoter that does not require induction. (sigmaaldrich.com)
  • We also offer a range of other bacterial promoters that are compatible with this plasmid and are available on request. (sigmaaldrich.com)
  • The term's early usage included any bacterial genetic material that exists extrachromosomally for at least part of its replication cycle, but because that description includes bacterial viruses, the notion of plasmid was refined over time to comprise genetic elements that reproduce autonomously. (wikipedia.org)
  • A typical bacterial replicon may consist of a number of elements, such as the gene for plasmid-specific replication initiation protein (Rep), repeating units called iterons, DnaA boxes, and an adjacent AT-rich region. (wikipedia.org)
  • Highly conserved regions of amino acid sequence were also found between ORF-B and other bacterial plasmids. (elsevierpure.com)
  • We have several standard primers for the most common vectors and for bacterial 16S rDNA sequencing. (helsinki.fi)
  • The plasmid encodes a Type III secretion system (T3SS) on a 30 kb pathogenicity island (PAI), and is maintained in a bacterial population through a series of toxin:antitoxin (TA) systems which mediate post-segregational killing (PSK). (ox.ac.uk)
  • The FDA's 2009 "Guidance on Prophylactic DNA Vaccines: Analysis and Recommendations" states that concerns about plasmid DNA potentially integrating into the genome of the vaccine recipient and increasing the likelihood of issues like "malignant transformation, genomic instability, or cell growth dysregulation" were raised when DNA vaccines were initially introduced for clinical use. (zerohedge.com)
  • Comparison of the complete genome sequences of Pseudomonas syringae pv. (nih.gov)
  • The Pss B728a genome (6.1 Mb) contains a circular chromosome and no plasmid, whereas the Pst DC3000 genome is 6.5 mbp in size, composed of a circular chromosome and two plasmids. (nih.gov)
  • Over 375 repetitive extragenic palindromic sequences unique to Pss B728a when compared with Pst DC3000 are widely distributed throughout the chromosome except in 14 genomic islands, which generally had lower GC content than the genome as a whole. (nih.gov)
  • The complete genome sequence of Archeoglobus fulgidus is published. (jcvi.org)
  • Helicobacter pylori genome sequence published. (jcvi.org)
  • Twenty years ago, President Bill Clinton announced completion of what was arguably one of the greatest advances of the modern era: the first draft sequence of the human genome. (jcvi.org)
  • Both scientists made waves after discovering plasmid DNA in the mRNA COVID-19 injections, warning it could potentially alter the human genome. (theepochtimes.com)
  • Mr. McKernan, a former researcher and team leader for the Massachusetts Institute of Technology Human Genome Project, told The Epoch Times he suspects Pfizer didn't disclose the presence of the DNA sequence due to the association of SV40 with polio vaccines. (theepochtimes.com)
  • He said while there is no evidence the sequence is carcinogenic, he has concerns about its integration into the human genome. (theepochtimes.com)
  • Complete genome and plasmid sequences of three Canadian isolates of Salmonella enterica subsp. (pacb.com)
  • See how the University of Washington used HiFi sequencing to uncover a key finding about ALS and the human genome. (pacb.com)
  • Here, we report the fully assembled genome and plasmid sequences of three S. Heidelberg strains with phage types 9, 29, and 41. (pacb.com)
  • Comparative genomics of several strains of Erwinia amylovora, a plant pathogenic bacterium causal agent of fire blight disease, revealed that its diversity is primarily attributable to the flexible genome comprised of plasmids. (udg.edu)
  • Whole genome sequencing by UPHL, a regional labora- bers and to collect environmental samples. (cdc.gov)
  • The sequence was a 93,629- bp plasmid encoding a single antimicrobial drug resistance gene, bla CTX-M-14. (cdc.gov)
  • The plasmid contains a constitutive promoter (OXB20) derived from the region upstream of the E. coli RecA gene. (sigmaaldrich.com)
  • This host-to-host transfer of genetic material is one mechanism of horizontal gene transfer, and plasmids are considered part of the mobilome. (wikipedia.org)
  • Plasmids almost always carry at least one gene. (wikipedia.org)
  • Integrated chromosomal and plasmid sequence analyses reveal diverse modes of carbapenemase gene spread among Klebsiella pneumoniae. (ox.ac.uk)
  • Whether you're designing plasmids, gene constructs, or cloning vectors, sequence editor provides a platform to build and annotate your molecular designs efficiently. (scinote.net)
  • We have determined the nucleotide sequence of the C gene. (shengsci.com)
  • However, the two share different degrees of concern about the significance of an SV40 sequence-which is used as an enhancer to drive gene transcription during the vaccine manufacturing process-being present in the shots. (theepochtimes.com)
  • The Neisseria gonorrhoeae assay uses LCR TM (ligase chain reaction) amplification technology in the LCx Probe System for the direct, qualitative detection of a specific target nucleic acid sequence in the Opa gene of Neisseria gonorrhoeae. (cdc.gov)
  • The four oligonucleotide probes in the LCx assay recognize and hybridize to a specific target sequence within the Opa gene of Neisseria gonorrhoeae DNA. (cdc.gov)
  • The bla NDM-1 gene was encoded on plasmids that were easily transferable. (who.int)
  • however, some classes of plasmids encode the conjugative "sex" pilus necessary for their own transfer. (wikipedia.org)
  • From this information, PCRs identifying novel features of pCT were designed and applied to isolates from several countries, showing that the plasmid has disseminated worldwide in bacteria from humans and animals. (cdc.gov)
  • The plasmid spread to unrelated E. coli isolates within an index cattle farm and persisted within the environment. (cdc.gov)
  • In this study, we report the full sequence and analysis of pCT and demonstrate the spread of pCT-like plasmids in animal and human E. coli isolates from the United Kingdom, Europe, Australia, and Asia. (cdc.gov)
  • In this study, we determined the fitness effects of the major antibiotic resistance plasmid pOXA-48_K8 in wild-type, ecologically compatible enterobacterial isolates from the human gut microbiota. (nature.com)
  • Here, we used a combination of long- and short-read sequence data of Klebsiella pneumoniae isolates (n = 1,717) from a European survey to perform an integrated, continent-wide study of chromosomal and plasmid diversity. (ox.ac.uk)
  • About 3,000 isolates have been selectively sequenced for different projects, Snesrud said. (genomeweb.com)
  • The mcr-1 on transferrable plasmids can instead be passed on to "other strains and other species that they come in contact with in the environment," he said. (genomeweb.com)
  • A duplex PCR assay was developed to survey the prevalence of plasmid pEI70 and also that of pEA29, which had previously been described in several E. amylovora strains. (udg.edu)
  • Restriction analysis and hybridization demonstrated that this plasmid was identical in at least 13 strains. (udg.edu)
  • Curing E. amylovora strains of pEI70 reduced their aggressiveness on pear, and introducing pEI70 into low-aggressiveness strains lacking this plasmid increased symptoms development in this host. (udg.edu)
  • Plasmids are extra-chromosomal mobile genetic elements able to transfer between bacteria through conjugation 1 . (nature.com)
  • Later in 1968, it was decided that the term plasmid should be adopted as the term for extrachromosomal genetic element, and to distinguish it from viruses, the definition was narrowed to genetic elements that exist exclusively or predominantly outside of the chromosome and can replicate autonomously. (wikipedia.org)
  • With the introduction of the sequence editor tool in SciNote electronic lab notebook , you now have a powerful and intuitive tool at your disposal for editing, annotating, and visualizing genetic sequences. (scinote.net)
  • Understanding the genetic makeup of plasmids is crucial in molecular biology and genetic research. (prmac.com)
  • Genetic plasticity of the Shigella virulence plasmid is mediated by intra- and inter-molecular events between insertion sequences. (ox.ac.uk)
  • The use of genetic engineering and modern techniques such as transcriptomics, proteomics and sequence analysis are included in the course. (lu.se)
  • The entire 127,923-bp sequence of the toxin-encoding plasmid pBtoxis from Bacillus thuringiensis subsp. (bgu.ac.il)
  • Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms. (wikipedia.org)
  • Plasmids are transmitted from one bacterium to another (even of another species) mostly through conjugation. (wikipedia.org)
  • A few types of plasmids can also insert into the host chromosome, and these integrative plasmids are sometimes referred to as episomes in prokaryotes. (wikipedia.org)
  • The isolated DNA can be used directly in standard applications, including automated sequencing, PCR, restriction analysis, and cloning. (qiagen.com)
  • Researchers can perform restriction enzyme analyses, edit sequences, create plasmid maps, cut and ligate "in silico," analyze GC content, make homology plots, and select primer sites from a user-specified database. (the-scientist.com)
  • In order for plasmids to replicate independently within a cell, they must possess a stretch of DNA that can act as an origin of replication. (wikipedia.org)
  • The normal number of copies of plasmid that may be found in a single cell is called the plasmid copy number, and is determined by how the replication initiation is regulated and the size of the molecule. (wikipedia.org)
  • De novo assembly of genuine replication forks on an immobilized circular plasmid in Xenopus egg extracts. (shengsci.com)
  • We describe an improved model of DNA replication in Xenopus egg extracts, in which a circular plasmid immobilized on paramagnetic beads is used as a template. (shengsci.com)
  • This ORF encodes a putative protein which is 360 aa residues in length and is 37.8% homologous to the replication protein of plasmid pCA2.4 from Synechocystis sp. (elsevierpure.com)
  • strain PCC 6803, 35.8% homologous to an ORF from the Nostoc plasmid pGL2, and 33.2% homologous to the ORF of a plasmid from Lactobacillus plantarum, pC30il. (elsevierpure.com)
  • Plasmids are generally circular, but examples of linear plasmids are also known. (wikipedia.org)
  • These linear plasmids require specialized mechanisms to replicate their ends. (wikipedia.org)
  • DNA synthesis occurred on either circular or linear plasmids coupled to the beads, but only DNA synthesis on the circular plasmid was inhibited by geminin and a. (shengsci.com)
  • Acquisition of a single copy, large virulence plasmid, pINV, led to the emergence of Shigella spp. (ox.ac.uk)
  • However, a common virulence plasmid was not identified in Providencia species. (medscape.com)
  • The tool works with standard single letter nucleotide or protein codes including ambiguities and can match Prosite patterns in protein sequences. (nih.gov)
  • We report here the complete nucleotide sequence (2561 bp) of this plasmid. (elsevierpure.com)
  • For reference most cloning programs will import a .gb (Genbank) file and will show all of the plasmids features automatically when downloaded and imported. (sigmaaldrich.com)
  • As part of global surveillance and sequencing of carbapenem-resistant Escherichia coli , we identified a sequence type 131 strain harboring bla IMP-14 within a class 1 integron, itself nested within an ∼54-kb multidrug resistance region on an epidemic IncA/C 2 plasmid. (ox.ac.uk)
  • the strain had 5 plasmids. (bvsalud.org)
  • The reason for the observed defective assembly in E. coli is probably not some unidentified mutation in the plasmid DNA because the same plasmid preparation complemented a B. subtilis sdhCAB deletion strain [7]. (lu.se)
  • 5 , 6 ), plasmids with the ability to spread efficiently, or epidemic plasmids, also are believed to be responsible for disseminating CTX-M ESBLs ( 7 ). (cdc.gov)
  • Dive into the research topics of 'Complete Sequence and Molecular Epidemiology of IncK Epidemic Plasmid Encoding blaCTX-M-14. (birmingham.ac.uk)
  • Complete DNA sequences can be used as a platform to develop rapid epidemiologic tools to identify and trace the spread of plasmids in clinically relevant pathogens, thus facilitating a better understanding of their distribution and ability to transfer between bacteria of humans and animals. (cdc.gov)
  • Plasmids are one of the primary sources for extrachromosomal DNA in bacteria and are capable of self-transmission. (frontiersin.org)
  • A plasmid is a strand of circular DNA that is common to bacteria and specific parasites. (zerohedge.com)
  • Plasmids can also provide bacteria with the ability to fix nitrogen. (wikipedia.org)
  • In fact, as reported by Drancourt et al (2004), genotyping performed on bacteria derived from the remains of plague victims of the first two epidemics revealed sequences similar to that of Orientalis. (medscape.com)
  • After ingestion of infected blood, the bacteria survive in the midgut of the flea owing to a plasmid-encoded phospholipase D that protects them from digestive juices. (medscape.com)
  • You can start by importing sequence files in .gb, .fasta, .dna formats or Teselagen JSON files into SciNote. (scinote.net)
  • Search for the sequence within SciNote by the sequence file name or by the file format type (.json, .dna, .fasta, .gb). (scinote.net)
  • Sequence export - Export sequences as a .gb, .fasta or Teselagen JSON files. (scinote.net)
  • Second, studies tend to analyse the fitness effects of a single plasmid in a single bacterium. (nature.com)
  • The particular plasmid type that contains the resistance elements is important to the epidemiology. (genomeweb.com)
  • Sequencing read lengths of over 700 bases are easily achieved. (qiagen.com)
  • Pfizer and Moderna COVID-19 mRNA vaccines "are contaminated with plasmid DNA fragments which have not been removed during the current manufacturing processes," the doctor wrote. (zerohedge.com)
  • It isn't clear whether the presence of the SV40 sequence in the plasmid DNA of mRNA vaccines was identified by Pfizer when the company applied for approval from the FDA. (zerohedge.com)
  • He said that since the average length of the degraded DNA fragments found by Dr. Buckhaults was 100 base pairs, and the SV40 enhancer sequence is only 72 base pairs long, the chances of finding an intact, fully functional SV40 enhancer sequence in the mRNA vaccines is "relatively high, certainly not negligible. (theepochtimes.com)
  • This plasmid is designed to express tagged proteins in E. coli. (sigmaaldrich.com)
  • The plasmid pSP65 which has cloned the complete HBV sequence, was amplified by transformation of coli (DH5). (bvsalud.org)
  • The operon on plasmid in E. coli was found to result in the production of all three subunit polypeptides [7]. (lu.se)
  • Plasmids are an important tool in molecular biology. (tmcnet.com)
  • Dr. Buckhaults said on social media the SV40 enhancer sequence taken from SV40 is a "standard bit of molecular biology engineering to achieve high level expression of the Neo resistance marker" and that it's been "used for decades. (theepochtimes.com)
  • The miniF plasmid C protein: sequence, purification and DNA binding. (shengsci.com)
  • A plasmid is a small, extrachromosomal DNA molecule within a cell that is physically separated from chromosomal DNA and can replicate independently. (wikipedia.org)
  • This plasmid contains an Outer Membrane Protein T (OmpT) secretory signal peptide (SP) to allow proteins to be exported from the cytosol. (sigmaaldrich.com)
  • The complete sequences of 50 new cDNA clones which code for large proteins. (nih.gov)
  • In addition to the four known Cry and two known Cyt toxins, a third Cyt-type sequence was found with an additional C-terminal domain previously unseen in such proteins. (bgu.ac.il)
  • We report the structures of the first fully sequenced blaOXA-163 plasmid and of two other blaOXA-48 plasmids in this lineage. (ox.ac.uk)
  • Find regions of similarity between this sequence and other sequences using BLAST. (nih.gov)
  • How does whole plasmid sequencing compare to Sanger sequencing? (eurofinsgenomics.com)
  • Sanger sequencing and Oxford Nanopore sequencing (ONT) are both methods for determining the sequence of nucleotides in a piece of DNA. (eurofinsgenomics.com)
  • Typically Sanger is consider the most accurate method for short-read sequencing and NGS is better for long-read sequencing. (eurofinsgenomics.com)
  • Overall, the choice between Sanger sequencing and ONT will depend on the specific needs of the application. (eurofinsgenomics.com)
  • These results help to explain the high prevalence of plasmids in the greatly diverse natural microbial communities. (nature.com)
  • Together with SciNote, this feature-rich tool simplifies the management of DNA and protein sequences, offering seamless integration within your research workflow. (scinote.net)
  • Dr. Malone said that prior FDA guidance on DNA vaccine technology pointed to the presence of "highly active regulatory sequences as being of particular concern due to potential insertional mutagenesis (integration). (zerohedge.com)
  • To address the gap of knowledge on this, the complete sequences of eight plasmids from Pandoraea spp. (frontiersin.org)
  • we will provide a clean, complete consensus sequence for each plasmid. (eurofinsgenomics.com)
  • The complete genomic sequence of Pseudomonas syringae pv. (nih.gov)
  • Complete sequencing and analysis of pEN2701, a novel 13-kb plasmid from an endophytic Streptomyces sp. (edu.au)
  • Complete sequence and organization of pBtoxis, the toxin-coding plasmid of Bacillus thuringiensis subsp. (bgu.ac.il)
  • Dive into the research topics of 'Complete sequence and organization of pBtoxis, the toxin-coding plasmid of Bacillus thuringiensis subsp. (bgu.ac.il)
  • Third, most mathematical models of plasmid population biology study clonal or near-clonal populations. (nature.com)
  • To date, information on plasmid analysis in Pandoraea spp. (frontiersin.org)
  • You can send it and we can sequence it, but we cannot predict or promise the analysis outcome. (eurofinsgenomics.com)
  • Sequence-Analysis Beerware DNA sequence analysis software needn't be expensive. (the-scientist.com)
  • For a decade, molecular biologist Kjeld Olesen has spent much of his spare time developing pDRAW32, a free sequence-analysis software package for Windows PCs. (the-scientist.com)
  • DNA sequence analysis software needn't be expensive. (the-scientist.com)
  • In an email to The Epoch Times last month, Health Canada confirmed the presence of the SV40 DNA sequence in the Pfizer vaccine, which the company failed to disclose previously. (zerohedge.com)
  • In another email to The Epoch Times, the European Medicines Agency also confirmed that the Pfizer vaccine contains the SV40 sequence, which the company's partner BioNTech did not highlight in its application. (zerohedge.com)
  • The health regulator says Pfizer did not disclose the presence of the Simian Virus 40 (SV40) DNA sequence in its mRNA COVID-19 vaccine at the time of filing. (theepochtimes.com)
  • Health Canada has confirmed the presence of a Simian Virus 40 (SV40) DNA sequence in the Pfizer COVID-19 vaccine, which the manufacturer had not previously disclosed. (theepochtimes.com)
  • Health Canada expects sponsors to identify any biologically functional DNA sequences within a plasmid (such as an SV40 enhancer) at the time of submission," the agency said in an email to The Epoch Times. (theepochtimes.com)
  • Although the full DNA sequence of the Pfizer plasmid was provided at the time of initial filing, the sponsor did not specifically identify the SV40 sequence. (theepochtimes.com)
  • The regulator said that after scientists Kevin McKernan and Dr. Phillip J. Buckhaults publicly raised the presence of SV40 enhancers in the vaccines earlier this year, "it was possible for Health Canada to confirm the presence of the enhancer based on the plasmid DNA sequence submitted by Pfizer against the published SV40 enhancer sequence. (theepochtimes.com)
  • Dr. Buckhaults, professor of cancer genomics and director of the Cancer Genetics Lab at the University of South Carolina, has tried to alleviate concerns about the presence of the SV40 enhancers, saying they pose a "small [cancer] hazard, but so do all the other pieces of plasmid DNA. (theepochtimes.com)
  • It's important for the public to realize that the SV40 sequence in the vaccine is NOT the cancer-causing SV40 large T antigen, which would be a very significant cancer risk," Dr. Buckhaults told The Epoch Times via email. (theepochtimes.com)
  • Dr. Patrick Provost, a professor in the Department of Microbiology, Infectious Diseases and Immunology at the Faculty of Medicine at Laval University, said the SV40 enhancer sequence was used in the vaccines because it's a component of the plasmid used for amplification of the vaccine mRNA. (theepochtimes.com)
  • Dr. Provost said the SV40 enhancer could still be in the final product if the plasmid DNA was not sufficiently degraded by DNases, which are meant to cut large DNA pieces into smaller ones. (theepochtimes.com)
  • deletions occur through intra-molecular recombination events between insertion sequences (ISs) flanking the PAI. (ox.ac.uk)
  • The Chlamydia trachomatis assay uses LCR TM (ligase chain reaction) amplification technology in the LCx Probe System for the direct, qualitative detection of plasmid DNA of Chlamydia trachomatis. (cdc.gov)
  • The HBV sequence was then labeled for its utilization as a probe in the HBV DNA detection in serum of patients with HBsAg positive, with no evident physical symptoms of the disease. (bvsalud.org)
  • ORFs and protein translations - Translate DNA sequences directly to amino acid sequences. (scinote.net)
  • The LCx Chlamydia trachomatis assay uses the nucleic acid amplication method LCR to detect the presence of C. trachomatis plasmid DNA directly in clinical specimens. (cdc.gov)
  • Plasmid pEI70 was found widely dispersed across Europe with frequencies of 5-92%, but it was absent in E. amylovora analyzed populations from outside of Europe. (udg.edu)
  • Plasmids are considered replicons, units of DNA capable of replicating autonomously within a suitable host. (wikipedia.org)
  • Properties such as GC-content, repeats such as poly(A)-tails and loop structures affect sequencing reactions, so any information you can provide will help us select suitable reaction protocol. (helsinki.fi)
  • If you want to use your own primers, take good care that you design them suitable for sequencing. (helsinki.fi)
  • Isolated plasmid DNA has OD 260 /280 ratios of 1.7-1.9, indicating that the quality and purity is suitable for all sensitive applications. (qiagen.com)
  • C protein binds strongly to pifO, and weakly to sequences in the ori-1 region. (shengsci.com)
  • Design and test primers for this sequence using Primer-BLAST. (nih.gov)
  • You can also use the toolbar to hide or show cut sites, features, parts, and primers in your map, thereby customizing how you view your sequence based on your needs. (scinote.net)