Sequenced protein expressed sequence tags. Medical search. Frequent questions

We identified zebrafish and Fugu TNFSF 13b homologues by genomic DNA database searches and determined that the predicted proteins share 68% and 76% sequence identity with trout TNFSF 13b. (usda.gov)
Three introns were identified in the rainbow trout, Atlantic salmon and zebrafish genomic sequences, and the intron locations correspond closely to those in the mammalian TNFSF 13b genes. (usda.gov)
We have catalogued the protein kinase complement of the human genome (the "kinome") using public and proprietary genomic, complementary DNA, and expressed sequence tag (EST) sequences. (nordiag.com)
We have shown that approximately 14% of the newly sequencedexpressed sequence tags map completely or partially to genomic regions where there are no annotated genes orsplice variants and therefore, imply that these are novel genetic structures. (sfu.ca)
The genomic organization of the human genes that encode protein prenyltransferases is shown in Figure 1 . (biomedcentral.com)
Therefore comparative genomic analysis of T . equi was undertaken to: 1) identify genes contributing to immune evasion and persistence in equid hosts, 2) identify genes involved in PBMC infection biology and 3) define the phylogenetic position of T . equi relative to sequenced apicomplexan parasites. (biomedcentral.com)
Comparative genomic analysis of T . equi revealed the phylogenetic positioning relative to seven apicomplexan parasites using deduced amino acid sequences from 150 genes placed it as a sister taxon to Theileria spp . (biomedcentral.com)
6% of the ESTs matched neither GenBank cDNA sequences nor the newly assembled chicken genomic DNA sequence, three ESTs aligned with sequences designated to be on the Z_random, while one matched a W chromosome sequence and could be useful in cataloguing functionally important genes on this sex chromosome. (westminster.ac.uk)
Here, we have described resources that may be useful in comparative and functional genomic analysis of genes expressed in an important organ, the pineal gland, in a model and agriculturally important organism. (westminster.ac.uk)
Several hundred partial genomic DNA sequences as nucleotide-binding-site leucine-rich repeat (NBS-LRR) resistance genes ( R ) have been identified, but a small portion with expressed transcripts has been found. (jipb.net)
Using information from most sequenced eukaryotic lineages, our phylogenetic analysis is based on full-length cDNA-, EST-derived-and genomic- PDZ and LIM domain sequences of over 25 species, ranging from yeast to humans. (princeton.edu)
Advances in genomic sequencing technology are transforming medical discovery, enabling researchers to conduct comprehensive explorations across whole genomes for individuals and populations. (cdc.gov)
Array comparative genomic hybridization (aCGH) is a type of microarray now routinely used to identify deleted or duplicated regions of DNA sequence in specific chromosomes on a genome-wide basis. (msdmanuals.com)
He also noted concern regarding delays in referring specimens to the laboratory for testing, and emphasized the need for improvement in timeliness of reporting and tracking of the viruses referred for genomic sequencing. (who.int)
He thanked the regional and global specialized laboratories for their efforts to provide timely results of the genomic sequencing of wild polioviruses, and for their help in planning and monitoring polio eradication activities in the Region. (who.int)

ESTs may be used to identify gene transcripts, and were instrumental in gene discovery and in gene-sequence determination. (wikipedia.org)
Because these clones consist of DNA that is complementary to mRNA, the ESTs represent portions of expressed genes. (wikipedia.org)
In this respect, ESTs have become a tool to refine the predicted transcripts for those genes, which leads to the prediction of their protein products and ultimately of their function. (wikipedia.org)
Because of the way ESTs are sequenced, many distinct expressed sequence tags are often partial sequences that correspond to the same mRNA of an organism. (wikipedia.org)
When the complete genome sequence of an organism is available and transcripts are annotated, it is possible to bypass contig assembly and directly match transcripts with ESTs. (wikipedia.org)
Sequencing of 16 M. persicae cDNA libraries generated 26,669 expressed sequence tags (ESTs). (nih.gov)
As a first step in pursuing this research, we report the generation and analysis of expressed sequence tags (ESTs) from the male accessory gland of H. erato and H. melpomene, species representative of the two mating systems present in the genus Heliconius. (ku.edu)
Results: We successfully sequenced 933 ESTs clustering into 371 unigenes from H. erato and 1033 ESTs clustering into 340 unigenes from H. melpomene. (ku.edu)
Conclusion: This collection of ESTs will serve as the foundation for the future identification and evolutionary analysis of male reproductive proteins in Heliconius butterflies. (ku.edu)
Automatic comparisons of data from expressed sequence tags (ESTs) with genes (for example using the program Acembly, for which the results are available from the NCBI AceView server [ 2 ]) shows that all the human protein prenyltransferase genes have multiple alternative splice variants. (biomedcentral.com)
Here, we describe the isolation and characterization of expressed sequence tags (ESTs) isolated from a chicken pineal gland cDNA library. (westminster.ac.uk)
Additionally, single nucleotide polymorphisms (SNPs) were identified and validated in 10 ESTs that showed 98% or higher sequence similarity to known chicken genes. (westminster.ac.uk)
We aimed to identify resistance gene analogs (RGAs) from peanut expressed sequence tags (ESTs) and to develop polymorphic markers. (jipb.net)
The protein sequences of 54 known R genes were used to identify homologs from peanut ESTs from public databases. (jipb.net)
A total of 1,053 ESTs corresponding to six different classes of known R genes were recovered, and assembled 156 contigs and 229 singletons as peanut-expressed RGAs. (jipb.net)

High-density oligonucleotide arrays were used to identify genes differentially expressed between high and low ERG expressers. (nih.gov)
One-third of the 471 genes predicted to encode transcription factors are differentially expressed during sexual development of S. commune . (nature.com)
Quantitative PCR and high-throughput methods, such as expressed sequence tag analysis, have been used for measuring gene expression and identifying subsets of elm genes that are differentially expressed in the presence of O. novo-ulmi . (sisef.it)
These analyses have also helped identify genes that were differentially expressed in DED fungi grown under defined experimental conditions. (sisef.it)

Among these tick salivary proteins are lipocalins, antiparallel beta-barrel proteins that sequester small molecules. (biomedcentral.com)

Here, we used a genomics/proteomics approach (including immunoblot experiments from pigs infected with T. suis ) to prioritize putative immunogenic excretory/secretory (E/S) proteins conserved across and specific to several gastrointestinal (GI) parasitic nematode species. (frontiersin.org)
In both species only a third of accessory gland unigenes were also found among genes expressed in wing tissue. (ku.edu)
About 25% of unigenes from both species encoded secreted proteins. (ku.edu)
To overcome the host plant defense response, M. phaseolina encodes a significant number of P450s, MFS type membrane transporters, glycosidases, transposases, and secondary metabolites in comparison to all sequenced ascomycete species. (biomedcentral.com)

The importance of S. commune as a model system is also exemplified by the fact that its recombinant DNA constructs will express in other mushroom-forming fungi 5 . (nature.com)
Monoclonal mouse Anti·His Antibodies (5xHis antibodies and 4xHis antibodies), like the RGS·His Antibody, BSA-free, Tetra·His Antibody, BSA-free and Penta·His Antibody, BSA-free are used to detect recombinant protein carrying His tags. (qiagen.com)
Although a recent study ( 22 ) reported enhanced avidity of H5N6 viruses for human-type receptors, recombinant clade 2.3.4.4 highly pathogenic influenza A virus H5 proteins from virus isolates in North America show a strict avian receptor-binding preference ( 23 ). (cdc.gov)
A cocktail of five recombinant proteins optimized for conserved GI nematode targets was used immunize pigs and test for active antibody responses in both the serum and intestinal ileal fluid of immunized pigs. (frontiersin.org)

Xpro is a relational database that contains all the eukaryotic protein-encoding DNA sequences contained in GenBank with associated data required for the analysis of eukaryotic gene architecture. (edu.au)
Three different protein prenyltransferases (farnesyltransferase and geranylgeranyltransferases I and II) catalyze the attachment of prenyl lipid anchors 15 or 20 carbons long to the carboxyl termini of a variety of eukaryotic proteins. (biomedcentral.com)
The chromosomal locations and number of exons from protein prenyltransferase genes in the major eukaryotic model organisms are shown in Table 2 . (biomedcentral.com)

EST approaches have largely been superseded by whole genome and transcriptome sequencing and metagenome sequencing. (wikipedia.org)
As we recently reported, analysis of 77 FHWT by whole genome sequencing (WGS, 58 patients) or whole exomic sequencing (WES,19 patients) identified 825 high-quality somatic, non-synonymous variants, with an average of 11 candidate mutations/case (range 2-42) (ref. 6 ). (nature.com)

Simple sequence repeats (SSRs) are highly variable features of all genomes. (biomedcentral.com)
Transcriptome analysis has facilitated the development of molecular markers such as simple sequence repeats (SSRs), single-nucleotide polymorphisms (SNPs) and potential intron polymorphisms (PIPs) that are being used to expedite the chickpea breeding programmes. (intechopen.com)
Twenty-eight simple sequence repeats (SSRs) were identified from 25 peanut expressed RGAs. (jipb.net)

This work alsodemonstrates the successful and efficient application of a next generation sequencing methodology.Results: We have generated over 30 million bases of novel expressed sequence tags from first larval stage wormsutilizing high-throughput sequencing technology. (sfu.ca)
Suchapplication of this new sequencing technique allows for high-throughput, genome-wide experimental verificationof known and novel transcripts. (sfu.ca)
We utilized a novel high throughput sequencing technology called 454 pyrosequencing which produced approximately 300,000 independent sequences. (usda.gov)
Aided by state-of-the-art, high-throughput computational methods, the current study simulated and analyzed the dynamics of competitive histamine binding at the tick-host interface using the available crystal structures of both the tick salivary lipocalin histamine-binding protein from Rhipicephalus appendiculatus and the human histamine receptor 1. (biomedcentral.com)

An antibody-protein array of putative immunogenic proteins was developed from a combined bioinformatic, experimental, and literature-based prioritization of homologous parasite proteins. (frontiersin.org)
Using this approach, we have generated a large amount of data forexpressed sequence tags, which provides an opportunity for the discovery of putative novel transcripts andalternative splice variants that could be developmentally specific to the first larval stage. (sfu.ca)
Each sequence from this database was used to search public protein databases to assign putative function to as many database entries as possible. (usda.gov)
Plasmodium parasites remodel their vertebrate host cells by translocating hundreds of proteins across an encasing membrane into the host cell cytosol via a putative export machinery termed PTEX (Plasmodium Translocon of EXported protein). (pberghei.eu)

This makes it difficult to write programs that can unambiguously determine that two EST libraries were sequenced from the same tissue. (wikipedia.org)
We have initiated study of the genes of the New World Screwworm, Cochliomyia hominivorax, by synthesizing and sequencing DNA libraries of expressed genes from embryos, 2nd instar larvae, adult males and adult females. (usda.gov)

Sequence analysis of the Daphnia pulex genome holds some surprises that could not have been anticipated from what was learned so far from other arthropod genomes. (biomedcentral.com)

Over the last two decades, several laboratories have used a wide range of techniques encompassing expressed sequence tag (EST) analysis, serial analysis of gene expression (SAGE), microarray and next-generation sequencing (NGS) technologies for analysing the chickpea transcriptomes. (intechopen.com)
These include previously unknown sequence data, a collection of expressed genes, molecular markers, and a DNA microarray that can be used to study aphid gene expression. (nih.gov)

In addition to the information found in the GenBank records, which includes properties such as sequence, position, length and description about introns, exons and protein-coding regions, Xpro provides annotations on the splice sites and intron phases. (edu.au)

As with other mitochondrial carrier family proteins, the ornithine carrier is composed of 300 amino acids that constitute 3 repeated motifs of approximately 100 amino acids each. (medscape.com)
The transporter was identified by probing a mammalian-expressed sequence tag database with 2 fungal mitochondrial ornithine carrier protein sequences. (medscape.com)
Mitochondrial and chloroplast proteins are therefore synthesized in the cytosol as preproteins with an NH 2 -terminal targeting sequence, which is both necessary and sufficient for directing the preproteins to the correct organelle. (silverchair.com)

Gene structures and chromosomal locations of human protein prenyltransferase subunit genes. (biomedcentral.com)
The precursor forms of prominent chloroplast proteins like the small subunit of stromal Rubisco (SSU), the thylakoid a/b binding protein (LHCP), or the thylakoid lumen localized subunits of the oxygen evolving complex of 23 kD and 33 kD (OE23, OE33) contain a phosphorylation site within the chloroplast targeting signal ( Waegemann and Soll 1996 ). (silverchair.com)
In the Fp subunit of SQR, and the closely related membrane-associated fumarate reductase of anaerobic organisms, FAD is covalently attached via an 8α-N(3)-histidyl linkage to a histidine (His) in a conserved sequence in the N-terminal part of the polypeptide. (lu.se)

Furthermore, Xpro validates intron positions using alignment information between the record's sequence and EST sequences found in dbEST. (edu.au)

In 1982, the idea of selecting random or semi-random clones from such a cDNA library for sequencing was explored by Greg Sutcliffe and coworkers. (wikipedia.org)
sequenced 178 clones from a rabbit muscle cDNA library. (wikipedia.org)
Anti·His Antibodies enable identification of positive expression clones by colony blotting (see figure Identification of positive expression clones by colony blotting. . "> "Identification of positive expression clones by colony blotting" ) and detection of His-tagged thioredoxin (see figure His-tagged thioredoxin detected by Penta·His Antibody in yeast cells. . "> "His-tagged thioredoxin detected by Penta·His Antibody in yeast cells" ). (qiagen.com)
Approximately 5,400 clones from each library were sequenced from both the 5' and 3' directions. (usda.gov)

Bortoluzzi S, Alessi FD, Romualdi C, Danieli GA: Differential expression of genes coding for ribosomal proteins in different human tissues. (karger.com)
Trout TNFSF 13b RNA is expressed in immune tissues including the spleen, anterior kidney, and peripheral blood leukocytes but not in the liver. (usda.gov)
Identification of intrahepatic cholangiocarcinoma related genes by comparison with normal liver tissues using expressed sequence tags. (nih.gov)

New insights into the genome have led to the emergence of proteomics , the study of the structure and function of an individual's entire set of expressed proteins. (cdc.gov)
Proteomics is highly linked to genomics, since the blueprint for each protein is inscribed on an organism's genes. (cdc.gov)
Some challenges for translating proteomics include technical issues in measuring proteomic markers, identifying clinically useful markers in patient samples, the need for large sample sizes to be able to distinguish protein variability among samples, and challenges in implementing strict protocols for sample handling. (cdc.gov)

They looked at more than 1100 base pairs of sequence from a chimpanzee, orangutan, macaque, and a dog, and they found that the coding sequence in humans has a small number of unexpected amino-acid-coding changes-changes that wouldn't be likely if the gene product was still being maintained by selection. (johnhawks.net)
The trout TNFSF 13b predicted protein shares 54% amino acid identity with human and chicken TNFSF 13b, and 51% identity with mouse TNFSF 13b. (usda.gov)
In particular, non-conservative amino acid substitutions in a highly expressed gut protease may be of adaptive significance for M. persicae feeding on different host plants. (nih.gov)
Importantly, the amino acid diversity of EMAs is distributed throughout the length of the proteins. (biomedcentral.com)

Expressedsequence tags, which map across intron-exon boundaries (around 300), indicate possible alternative splice sites,while expressed sequence tags, which map near the ends of known transcripts (around 600), suggest extensionof the coding or untranslated regions. (sfu.ca)

These motifs contain 2 hydrophobic alpha-helical segments connected by an extensive hydrophilic sequence, resulting in 6 transmembrane portions of the protein. (medscape.com)
There were 69 that encoded for NBS-LRR proteins, 191 that encoded for protein kinases, 82 that encoded for LRR-PK/transmembrane proteins, 28 that encoded for Toxin reductases, 11 that encoded for LRR-domain containing proteins and four that encoded for TM-domain containing proteins. (jipb.net)

DNA sequence and analysis of human chromosome 9. (nih.gov)

Concerted evolution refers to the pattern in which copies of multigene families show high intraspecific sequence homogeneity but high interspecific sequence diversity. (biomedcentral.com)

They may be represented in databases as either cDNA/mRNA sequence or as the reverse complement of the mRNA, the template strand. (wikipedia.org)
2014). Where annotations were not present or were different, the online Softberry server (http://linux1.softberry.com/berry.phtml) was used to predict mRNA and protein sequences using the FGNESH+ prediction algorithm (Solovyev et al. (molquest.com)

Comparison of cDNA data from three aphid lineages identified single nucleotide polymorphisms that can be used as genetic markers and, in some cases, may represent functional differences in the protein products. (nih.gov)
Recent advances in sequencing technology make it possible to comprehensively catalog genetic variation in population samples, creating a foundation for understanding human disease, ancestry and evolution. (nordiag.com)
Analysis of genetic diversity and population structure of peanut cultivars and breeding lines from China, India and the US using simple sequence repeat markers [J]. J Integr Plant Biol. (jipb.net)
Although amenable to genetic tagging, HSP101, PTEX150, EXP2 and PTEX88 could not be genetically deleted in P. berghei, in keeping with the obligatory role this complex is postulated to have in maintaining normal blood-stage growth. (pberghei.eu)
Next-generation sequencing technologies have dramatically changed the approach to genetic diagnosis. (msdmanuals.com)
This revolutionary and rapidly evolving technology has moved a significant portion of the technical aspects of genetic diagnosis to next-generation sequencing and has become the mainstay of genetic diagnosis. (msdmanuals.com)

In order to clarify the biological and pharmacological mechanisms of O. sinensis , we carried out the genome sequencing of H. sinensis, anamorph of O. sinensis , for the first time, described the annotation and gene expression, and analyzed the complete sequencing data (Li et al. (springeropen.com)

An EST results from one-shot sequencing of a cloned cDNA. (wikipedia.org)
However, the sheer volume of information generated by sequencing the exome or genome results in a variety of interpretive problems that complicate understanding of the results. (msdmanuals.com)

Single-gene knockout-coupled omics analysis identifies C9orf85 and CXorf38 as two uncharacterized human proteins associated with ZIP8 malfunction. (nih.gov)
Analysis of Resistance to Powdery Mildew in Wheat Based on Expressed Sequence Tags (EST) Technique [J]. J Integr Plant Biol. (jipb.net)
Until recently, however, functional analysis of the DED fungi was hampered by the lack of protocols for efficient gene knockout and by the unavailability of a full genome sequence. (sisef.it)

To gain deeper insight into the biological and pharmacological mechanisms, we sequenced the genome of H. sinensis . (springeropen.com)
The life cycle of a protein starts with a blueprint transcribed from a gene, followed by folding, which translates the blueprint to a three-dimensional structure that allows it to carry out its biological function, and ends with degradation. (cdc.gov)

In an effort to reduce the number of expressed sequence tags for downstream gene discovery analyses, several groups assembled expressed sequence tags into EST contigs. (wikipedia.org)
Some of these sequences could be assembled into larger composite sequences (termed contigs) based on overlapping sequence identity while other sequences had no overlapping sequence identity with any other members of the database and remained as unassembled singletons. (usda.gov)
Sequences were assembled into a database of assembled contigs and singletons and used to search public protein databases and annotate the sequences. (usda.gov)
The full database consists of 6,076 contigs and 58,221 singletons assembled from both the traditional EST and 454 sequences. (usda.gov)

The tagged DNA binds to its complementary DNA segment and can be detected by measuring the amount and type of fluorescence. (msdmanuals.com)

Each of the three variants detects a different His tag epitope. (qiagen.com)
Each mutation was verified by Sanger sequencing of PCR products spanning the variants ( Fig. 1 ). (nature.com)

The human coding sequence of MYH16 has a frameshift mutation that prevents translation of the functional product. (johnhawks.net)

In 1991, Adams and co-workers coined the term EST and initiated more systematic sequencing as a project (starting with 600 brain cDNAs). (wikipedia.org)

Hansell Stedman and collaborators at the University of Pennsylvania had collected sequence data for a gene called MYH16 . (johnhawks.net)
A framework for variation discovery and genotyping using next-generation DNA sequencing data. (nordiag.com)
A Better Way to Find RNA Virus Needles in the Proverbial Database Haystacks Researchers combed through more than 5,000 data sets of RNA sequences generated from diverse environmental samples around the world, resulting in a five-fold increase of RNA virus diversity. (doe.gov)
Three explanatory types of data can be obtained from the current study: (i) the dynamics of multiple binding sites, (ii) competition between two proteins for a ligand, and (iii) the intrinsic molecular components involved in the competition. (biomedcentral.com)

The α subunits of protein prenyltransferases consist of tetratricopeptide repeats and are part of the tetratricopeptide repeat superfamily [ 5 ], which also includes functionally diverse proteins involved in transcription, co-chaperoning, protein transport, cell-cycle control and phosphorylation. (biomedcentral.com)

As conventional alignment and phylogenetic procedures of full-length sequences fell short of elucidating the evolutionary history of these genes, we started to analyze the PDZ and LIM domain sequences themselves. (princeton.edu)

Genes contributing to the similarity include ribosomal protein (RP) genes as well as genes involved in transcription, translation and cell division. (karger.com)

Extensive analyses of transcriptome have been carried out in chickpea, which is the third most important legume valued as a source of dietary protein and micronutrients. (intechopen.com)

To expose any impact on export by ER retention of HSP101 masked during in vitro culture of P. falciparum blood stages, we tagged HSP101 and PTEX150 with kER in a transgenic P. berghei rodent malaria expressing DiCre and evaluated the impact of ER retrieval on parasite survival during mouse infection. (pberghei.eu)

For the sensitivity of the three Anti·His Antibodies in detecting this panel of proteins see the figure Sensitivity of anti·His antibodies. Detection of 6xHis-tagged proteins with Anti·His Antibodies (Tetra·His Antibody in the center). A: DHFR; B: DHFR; C: thioredoxin; D: TNF-α; E: TFIIA γ ; F: chaperonin; G DNA polymerase; H: TFIIAαß; for tag location and sequences detected see table 'Proteins detected with QIA express Anti·His Antibodies'. Indicated amounts of pure 6xHis-tagged protein were applied to a nitrocellulose membrane, and detection was carried out with the Anti·His primary antibody indicated diluted 1/2000, followed by chromogenic detection with AP-conjugated rabbit anti-mouse IgG and NBT/BCIP. "> "Sensitivity of QIA express Anti·His Antibodies" ). (qiagen.com)
If the membrane-anchor is missing or defective, Fp and Ip subunits accumulate as soluble proteins in the cytoplasm. (lu.se)

Streamlining Regulon Identification in Bacteria Regulons are a group of genes that can be turned on or off by the same regulatory protein. (doe.gov)
These expressed RGAs are the source for RGA-tagged marker development and identification of peanut resistance genes. (jipb.net)
Liu Z, Feng S, Pandey MK, Chen X, Culbreath AK, Varshney RK, Guo B (2013) Identification of expressed resistance gene analogs from peanut ( Arachis hypogaea L.) expressed sequence tags. (jipb.net)

By comparing the sequences of humans and other primates, Stedman's team estimated that MYH16 had lost its function in human ancestors around 2.4 million years ago. (johnhawks.net)
There are three different protein prenyltransferases in humans: farnesyltransferase (FT) and geranylgeranyltransferase 1 (GGT1) share the same motif (the CaaX box) around the cysteine in their substrates, and are thus called CaaX prenyltransferases, whereas geranylgeranyltransferase 2 (GGT2, also called Rab geranylgeranyltransferase) recognizes a different motif and is thus called a non-CaaX prenyltransferase [ 1 ]. (biomedcentral.com)

Furthermore, it can be cross-linked in intact chloroplasts to a high molecular weight complex containing both Toc and Tic subunits and a precursor protein. (silverchair.com)
To date, molecular dynamics simulations focus on protein-protein and protein-ligand interactions, but there are currently no studies for simultaneous ligand exploration between two competing proteins. (biomedcentral.com)
By cataloguing the molecular processes by which cells maintain and modify protein levels, proteomic studies offer another dimension of information that may help advance our understanding of health and disease. (cdc.gov)

In genetics, an expressed sequence tag (EST) is a short sub-sequence of a cDNA sequence. (wikipedia.org)
Overview of Genetics A gene, the basic unit of heredity, is a segment of DNA containing all the information necessary to synthesize a polypeptide (protein) or a functional RNA molecule. (msdmanuals.com)

For instance, cancer origin of a library is often mixed with the tissue name (e.g., the tissue name "glioblastoma" indicates that the EST library was sequenced from brain tissue and the disease condition is cancer). (wikipedia.org)

After overnight ex vivo culture to allow for tag switching, equal numbers of DMSO or rapamycin-treated parasites were IV injected into naïve mice. (pberghei.eu)

The mutant MLLT1 protein shows altered binding to acetylated histone tails. (nature.com)
The mutant expresses PTEX150 tagged C-terminal with a KDEL ER-retrieval sequence. (pberghei.eu)

The PDZ and LIM domain-containing protein family is encoded by a diverse group of genes whose phylogeny has currently not been analyzed. (princeton.edu)

The protein kinase complement of the human genome. (nordiag.com)
In 2014, scientists developed a draft map of the human proteome , which catalogued proteins encoded by over 17,000 human genes, or about 84% of all protein-coding genes in the human genome. (cdc.gov)

The resulting sequence is a relatively low-quality fragment whose length is limited by current technology to approximately 500 to 800 nucleotides. (wikipedia.org)
This technology involves breaking the entire genome into small segments, sequencing the segments, and then reassembling the sequences using intensive computational techniques to provide the base-by-base sequence of the entire genome or more limited regions, such as the expressed portion of the genome known as the exome. (msdmanuals.com)

METHODS: To identify expressed TNFSF genes, we searched a computer database of ~50,000 rainbow trout expressed sequence tags generated at the National Center for Cool and Cold Water Aquaculture. (usda.gov)

The Fp and Ip subunits are both very conserved with respect to composition and polypeptide sequence [1]. (lu.se)

Anatomy9

Organisms24

Chemicals and Drugs22

Analytical, Diagnostic and Therapeutic Techniques and Equipment23

Phenomena and Processes71

Disciplines and Occupations4

Technology, Industry, Agriculture5

Information Science13

Health Care2

Genomic15

ESTs15

Differentially expressed4

Salivary Proteins1

Species4

Recombinant4

Eukaryotic3

Whole genome2

Simple sequenc3

High-throughput4

Putative4

Libraries2

Genomes1

Microarray2

Exons1

Mitochondrial3

Subunit3

DbEST1

Clones4

Tissues3

Proteomics3

Amino4

Intron1

Transmembrane2

Chromosome1

Multigene1

MRNA2

Genetic6

Annotation1

Results2

Analysis3

Biological2

Contigs4

Complementary1

Variants2

Mutation1

CDNAs1

Data4

Functionally1

Phylogenetic1

Similarity1

Analyses1

Malaria1

Membrane2

Identification3

Humans2

Molecular3

Genetics2

Tissue1

Parasites1

Mutant2

Genes whose1

Human genome2

Technology2

Methods1

Polypeptide1