• Click molecule labels to explore molecular sequence information. (nih.gov)
  • Physical characterization of the protein (BsDHFR) indicates that it is a monomeric protein with a molecular mass of 18,694.6 Da (0.8), coincident with the mass of 18 694.67 Da calculated from the primary sequence. (duke.edu)
  • The T4 Gene 32 Protein is intended for molecular biology applications. (qiagen.com)
  • UQ's molecular clamp technology was designed to hold the spike protein in its original form to produce a useful subunit vaccine. (nature.com)
  • This was the impetus for building the molecular infrastructure to maintain a spike protein in its pre-fusion form. (nature.com)
  • These two conformations therefore act like a molecular switch to direct the fate of the viral RNA, and thus viral replication. (europa.eu)
  • A combination of molecular simulation with hydrogendeuterium-exchange mass spectrometry and electron paramagnetic resonance spectroscopy reveal an asymmetry across the membrane: ATP-induced conformational changes in the cytosolic cavity promote unfolded pre-protein structure, while the exterior cavity favours its formation. (elifesciences.org)
  • The methodology will include site-directed mutagenesis, covalent protein modifications, patch- clamp measurements, thermodynamic and kinetic analysis of dose-response curves and rates of transitions, videomicroscopy, and molecular modeling. (usda.gov)
  • Following these data, in collaboration with Dr. H. R. Guy (NIH), we built molecular models for the E. coli MscL in the closed, closed-expanded and open conformations and found that the channel can not be gated solely with gate that is placed within the transmembrane domain as was previously proposed (Spencer et al. (usda.gov)
  • Identifying the molecular states relevant to protein functioning is necessary for our understanding of biological processes. (biorxiv.org)
  • Small (~1-10 nm) protein-based components of the cell such as enzymes, MHC carriers, and ribosomes are self-assembling or require only modest assistance (e.g., molecular chaperones) to self-assemble. (nanomedicine.com)
  • Reversible mechanical denaturation of these smaller proteins, using diamondoid probe structure to displace water molecules (thus reducing hydrophobic forces) and by using specialized handling tools akin to functionalized AFM tips and molecular clamps, may be followed by precise nondestructive amino acid sequencing ( Chapter 20 ) to allow identification and diagnostic compositional analysis. (nanomedicine.com)
  • The course covers both the principles that determine the properties of proteins and the experimental methods that are used to study these properties in modern molecular protein science. (lu.se)
  • This is thought to make globular proteins stable and soluble in the watery environment of the cell. (anl.gov)
  • Resonance Group, Chemical of the globular proteins bovine pancreatic trypsin inhibitor (BPTI) and Center, Lund University ubiquitin in aqueous solution. (lu.se)
  • This difference was ascribed to the abscence, in ubiquitin, of highly ordered internal water molecules, which are known to be present in BPTI and in most other globular proteins. (lu.se)
  • Often these side chains appear at the surfaces on proteins to enable their solubility in water, and side chains with opposite charges form important electrostatic contacts called salt bridges that maintain structures within a single protein or between interfacing proteins. (wikipedia.org)
  • Many proteins bind metal into their structures specifically, and these interactions are commonly mediated by charged side chains such as aspartate, glutamate and histidine. (wikipedia.org)
  • Tetrapetide sequences and structures were expressed by letter codes. (medscimonit.com)
  • RESULTS: High values of the rho-coefficient extracted sequences of strong structural determinability and structures of high sequence selectivity. (medscimonit.com)
  • While other trimerization proteins, such as foldon from the T4-phage fibritin, were tried, the resulting structures were unstable and wouldn't keep well in storage. (nature.com)
  • Proteins from thermophilic organisms must retain their native structures under extreme conditions, where their homologues from mesophilic organisms denature. (berkeley.edu)
  • We proposed that a cell theoretically could in principle synthezise a big number of forms of [ 2 H]labeled macromolecules with somewhat different structures and conformations, so that a cell could easily select a preferable one from al these species in a course of adaptation to 2 H 2 O, that is the best suitable namely for that conditions. (o8ode.ru)
  • Called FARS-seq for short, it investigates the sequences and structures in HIV-1 that play an important role in dimerisation and genome packaging. (europa.eu)
  • Aided by FARS-seq, the team comprehensively identified sequences and structures within the 5′ untranslated region of HIV-1 messenger RNA that regulate the equilibrium between the two conformations. (europa.eu)
  • In general, immunobiologists have developed an integrated method for vaccine development based on analyzing protein sequences and structures of target viruses [ 10 ]. (biomedcentral.com)
  • However, only few protein structures have been currently resolved for the Iridoviridae family, none of which are associated with the outer capsid proteins. (biomedcentral.com)
  • Afterward, the protein molecule may be refolded back into its original minimum-energy conformation, possibly with the assistance of chaperone-like structures in some cases. (nanomedicine.com)
  • The challenges of crystallizing membrane proteins-and how they're being overcome Computer artwork of a G protein-coupled receptor in the lipid bilayer of a plasma membrane Medi-Mation Ltd / Photo Researchers Membrane proteins represent only a handful of the total number of protein structures defined to date. (the-scientist.com)
  • Membrane proteins represent only a handful of the total number of protein structures defined to date. (the-scientist.com)
  • X-ray crystallography-still the only general method for solving the atomic structures of proteins of any size-has been hampered by the extreme difficulty of preparing and crystallizing pure membrane proteins. (the-scientist.com)
  • Differences in the observed inhibitor conformation between the yeast and human structures are caused by differing residues in the binding pocket. (duke.edu)
  • Current hypotheses on protein folding suggest that folding is initiated by formation of secondary structures followed by association of the hydrophobic regions of the emerging structure to orient them toward the interior of the protein in a process termed hydrophobic collapse. (anl.gov)
  • The general aim of the course is to enable students to acquire an advanced understanding of proteins with an emphasis on their three-dimensional structures, the connection of structures to biological function and how these structures are produced. (lu.se)
  • The three S1 and S2 subunits form a fusion spike embedded in the viral membrane and held in a very particular conformation until it binds to a target cell. (nature.com)
  • 5 Another approach is to insert mutations that alter the protein sequence in a manner that stabilizes one particular conformation, and screen to find those that might rigidify otherwise flexible regions. (the-scientist.com)
  • Although ApoA-I amyloidosis is systemic, the different amyloidogenic variants show a preferential tissue accumulation that appears to correlate with the location of the mutation in the protein sequence and with the local extracellular microenvironment. (lu.se)
  • These diseases are caused by refolding of the cellular prion protein (PrP C ) into an infectious isoform (PrP Sc ) that catalytically templates its abnormal conformation onto additional molecules of PrP C ( Prusiner, 1998 ). (elifesciences.org)
  • However, when the adaptive process goes continuously during the many generation, the population of cells can use a special genetic mechanisms for the adaptation to 2 H 2 O. For example, mutations of geens can be resulted in amino acid replacements in molecules of proteins, which in turn could cause a formation of a new isoenzymes, and in the special cases - even the anomal working enzymes of a newer structure type. (o8ode.ru)
  • Yet these proteins, which represent nearly 40 percent of all known proteins, including receptors, channels, and signaling molecules, are essential for cell communication and their malfunctions are implicated in many diseases. (the-scientist.com)
  • Stevens collaborated with chemists to design new detergents, such as the cholate- based amphiphiles, that create smaller micelles, allowing the protein molecules to pack more closely and form better crystals. (the-scientist.com)
  • These observations demonstrate that the water relaxation dispersion in protein solutions is essentially due to a few structural water molecules buried within the protein matrix, but exchanging rapidly with the external water. (lu.se)
  • The water molecules residing at the surface of the two proteins were found to be highly mobile, with an average rotational correlation time of approximately 20 picoseconds. (lu.se)
  • We believe that the present results resolve the long-standing controversy regarding the mechanism behind the spin relaxation dispersion of water nuclei in protein solutions, thus establishing oxygen-17 relaxation as a powerful tool for studies of structurally and functionally important water molecules in proteins and other biomolecules. (lu.se)
  • Large macromolecules, including proteins and their complexes, very often adopt multiple conformations. (biorxiv.org)
  • Large macromolecules, including proteins and their complexes, are intrinsically flexible, and this flexibility is often linked with their function. (biorxiv.org)
  • Although over 500 amino acids exist in nature, by far the most important are the 22 α-amino acids incorporated into proteins. (wikipedia.org)
  • In the form of proteins, amino acid residues form the second-largest component (water being the largest) of human muscles and other tissues. (wikipedia.org)
  • Beyond their role as residues in proteins, amino acids participate in a number of processes such as neurotransmitter transport and biosynthesis. (wikipedia.org)
  • Proteins were found to yield amino acids after enzymatic digestion or acid hydrolysis. (wikipedia.org)
  • Rarely, D-amino acid residues are found in proteins, and are converted from the l-amino acid as a post-translational modification. (wikipedia.org)
  • somewhat similar sequences of about 110 amino acids each. (wikipedia.org)
  • The long-term goal of our research is to understand the structural and dynamic information encoded in the linear sequence of amino acids. (berkeley.edu)
  • We have found that although protein stability can be altered by single amino acid substitution, evolution for optimal function requires more subtle and delocalized mechanisms.Recent results implicate structure in the unfolded state as playing an important and novel role for the thermostability of these proteins. (berkeley.edu)
  • The mean score of a random protein conformation is estimated by a weighted sum of protein composition over the 20 standard amino acid residue types, where each weight corresponds to the expected change in the score by inserting a specific type of amino acid residue. (salilab.org)
  • Interested in the mechanism of a CLC chloride transporter protein, they tried to improve the data they obtained from their crystal by replacing a naturally occurring amino acid with a methionine at each of 30 sites in the protein. (the-scientist.com)
  • How does an amino acid sequence that is unfolded when it leaves the ribosome manage to fold properly into a highly ordered, lightning-fast enzyme or sturdy structural protein? (anl.gov)
  • However, previous work by this team of researchers from the University of Notre Dame and The University of Chicago has shown that, contrary to popular belief, intrinsically disordered proteins, which sample a variety of conformational forms the way the autotransporter proteins do, can remain quite extended in water even if they have high ratios of hydrophobic to charged amino acids. (anl.gov)
  • The first step was to analyze the impact of the sequence of the amino acids in the passenger domain on the folding process. (anl.gov)
  • They made different versions of the pertactin domain by swapping amino acids to change the clustering of hydrophobic amino acids but keep the overall sequence and hydrophobicity ratio the same. (anl.gov)
  • This finding suggests that spacing of hydrophobic amino acids must be under selective pressure in bacterial proteins to facilitate their virulence functions. (anl.gov)
  • Native or wild-type quaternary protein structure is usually born from a single translated protein sequence with one ordered conformation with downstream protein interactions. (medscape.com)
  • [ 4 ] It is also important to understand that the same polypeptide sequence can produce many different patterns of interresidue or intraresidue interactions. (medscape.com)
  • Pathogenic Signal Sequence Mutations in Progranulin Disrupt SRP Interactions Required for mRNA Stability. (academictree.org)
  • The study of MscL, a highly convenient model system, will give us basic understanding and the first example of what type of intramolecular interactions, sequence motifs, and conformations make membrane proteins sensitive to a physiologically relevant mechanical stimulation. (usda.gov)
  • Moreover, targeting protein functional motions bears a great potential to control and modulate proteins' activities and interactions in physio-pathological contexts. (biorxiv.org)
  • These techniques provide valuable insights on proteins' functioning and interactions with their environment. (biorxiv.org)
  • Protein folding, going from sequence to structure by mini- a quadratic Hamiltonian requires additional spin variables and mizing an energy function, represents a difficult optimization implementing interactions such as self-avoidance becomes problem. (lu.se)
  • We have obtained 50 trajectories for each protein and followed the free-energy profile and the order of folding of secondary structure elements between the last occurrence of the completely unfolded state and the first occurrence of the completely folded state. (nih.gov)
  • A new inhibited conformation of the KD is observed in a DFG-out conformation and with the glycine-rich loop adopting a structure that blocks ATP binding to the active site. (rcsb.org)
  • As such, they represent a useful system with which to study the role that turn sequences play in determining structure, stability, and folding of the protein. (rcsb.org)
  • Two turns related by the threefold structural symmetry, the beta4/beta5 and beta8/beta9 turns, were subjected to both sequence-swapping and poly-glycine substitution mutations, and the effects upon stability, folding, and structure were investigated. (rcsb.org)
  • The results indicate that the beta-turn structure at these positions is not determined by the turn sequence. (rcsb.org)
  • A tabular approach to the sequence-to-structure relation in proteins (tetrapeptide representation) for de novo protein design. (medscimonit.com)
  • The sequence-to-structure and structure-to-sequence relation is critical for predicting protein structure. (medscimonit.com)
  • CONCLUSIONS: The results revealed sequence-to-structure (and vice versa) correlation in early-stage folding. (medscimonit.com)
  • The T4 Gene 32 Protein has exhibited an ability to enhance the performance of several DNA synthesis-related activities in secondary-structure rich regions, including PCR amplification and DNA sequencing. (qiagen.com)
  • The bundle structure stabilizes the spike protein in its pre-fusion conformation. (nature.com)
  • By looking at the crystal structure of one D770_N771insNPG mutant, the group noted that the binding pocket is unaltered, but that the extra sequence creates a sort of wedge that keeps the protein in an active conformation. (genomeweb.com)
  • For instance, many proteins share the same native structure yet their cellular dynamics and function, in other words their energy landscapes, are different. (berkeley.edu)
  • 2016). Here, we show that ATP enhances this process by modulating secondary structure formation within the translocating protein. (elifesciences.org)
  • This ability to exploit structure within a pre-protein is an unexplored area of protein transport, which may apply to other protein transporters, such as those of the endoplasmic reticulum and mitochondria. (elifesciences.org)
  • B) Side and top views of the prefusion structure of the 2019-nCoV S protein with a single RBD in the up conformation. (forbes.com)
  • dihedral angle restraints will depend on the conformation of the equivalent disulfides in the template structure, as described in [ Šali & Overington, 1994 ]. (salilab.org)
  • Our method guaranties preservation of the protein structure during the transition and allows to access conformations that are unreachable with classical normal mode analysis. (biorxiv.org)
  • This allows us to produce a wide range of motions, some of them previously inaccessible, and to preserve the structure of the protein during the motion. (biorxiv.org)
  • Nevertheless, experimental protein structure determination remains a time consuming and costly process. (biorxiv.org)
  • One example comes from Raymond Stevens and colleagues at the Scripps Research Institute who discovered that lipids were essential for determining the structure of a G protein-coupled receptor (GPCR) that responds to adrenaline. (the-scientist.com)
  • They then tagged the methionines with the heavy element selenium, which helped them confirm the atomic structure of the protein with better precision. (the-scientist.com)
  • A secondary structure model of PutP from Escherichia coli suggests the protein contains 13 TMs with the N terminus located on the periplasmic side of the membrane and the C terminus facing the cytoplasm. (embl.de)
  • Folding a protein sequence into its minimum-energy structure represents such a problem. (lu.se)
  • Binding to a receptor triggers a very rapid structural change, with some parts of the protein flipping through 180°, pulling the viral membrane and the host membrane close together to allow them to fuse. (nature.com)
  • The trouble is that the final stable post-fusion form of the protein is not what is on the viral surface and so is not an appropriate form to stimulate a strong protective immune response in a vaccine. (nature.com)
  • In the second conformation, the RNA remains in the host cell to be translated into new viral proteins. (europa.eu)
  • To identify novel KSHV antiviral targets, we propose to identify virus-specific protein functions and protein conformations by employing highly specialized electron microscopy methods to directly visualize purified KSHV DNA replication proteins and viral DNA sequences during the formation of the DNA replication fork at the viral origins of replication. (nccu.edu)
  • AMP-activated protein kinase (AMPK) is a master metabolic regulator for controlling cellular energy homeostasis. (rcsb.org)
  • RNA-Seq analysis and the data from experimental models revealed that neutrophilic NCF1-dependent ROS promoted alcoholic hepatitis (AH) by inhibiting AMP-activated protein kinase (a key regulator of lipid metabolism) and microRNA-223 (a key antiinflammatory and antifibrotic microRNA). (jci.org)
  • BACKGROUND: : Inherited mutations in the CDKN2A tumor suppressor gene, which encodes the p16(INK4a) protein, and in the cyclin-dependent kinase 4 (CDK4) gene confer susceptibility to cutaneous malignant melanoma. (lu.se)
  • It had been shown that certain altered PrP proteins caused the death of brain cells by allowing excessive electrical charges to cross the membranes of the cell. (elifesciences.org)
  • Transport of proteins across membranes is a fundamental process, achieved in every cell by the 'Sec' translocon. (elifesciences.org)
  • The encapsulation and compartmentalisation of cells has necessitated the evolution of machineries that conduct proteins across membranes, including for protein secretion and organellar import. (elifesciences.org)
  • Because swabs from previous standard-of-care respiratory surveillance efforts should focus on whole-genome pathogen testing at Valleywise Health Medical Cen- sequencing to better understand the evolution of the ter, which serves Maricopa County, Arizona, USA. (cdc.gov)
  • The analysis of mutations now allows us to narrow the search for functionally important regions to the short N-terminal helix, two transmembrane domains of the protein, and the loop between them. (usda.gov)
  • The average hydropathy plot for SSSF proteins predicts 11 to 15 putative transmembrane domains (TMs) in alpha-helical conformation. (embl.de)
  • The backbone conformation has been experimentally recognized as responsible for the early-stage structural forms of a polypeptide. (medscimonit.com)
  • MATERIAL/METHODS: The polypeptide chains of all the proteins in the Protein Data Bank were transformed into their early-stage structural forms. (medscimonit.com)
  • I want to get the latest chemistry news from C&EN in my inbox every week. (acs.org)
  • Gp32 binds transiently and cooperatively to ssDNA template sequences as these entities are exposed by the processive helicase operating within the replication complex. (qiagen.com)
  • Most host antibodies target 6 an- consensus sequences using SAMtools version 1.17 tigenic sites (Ø-V) of the F protein ( 10 ). (cdc.gov)
  • Antibodies are heavy (~150 k Da ) proteins of about 10 nm in size, [7] arranged in three globular regions that roughly form a Y shape. (wikipedia.org)
  • That study showed that in naturally infected individuals the majority of potent neutralizing antibodies were those responding to the pre-fusion form of the protein, not the post-fusion form. (nature.com)
  • Rapid and comprehensive analysis of putative proteinous allergens ( allergenome ) by applying such a proteomic strategy … With allergenomics, we can not only detect and assign the putative allergens (proteins specifically interacting with IgE antibodies in a patient's blood) in a short time, but also analyze the quantitative and qualitative change of the antigens, depending on the surroundings and environmental conditions of an allergenic causative. (genomicglossaries.com)
  • These antibodies have been directed at the Spike protein on the virus's exterior. (forbes.com)
  • This is a favored target, as studies show that greater than ninety percent of naturally-occurring antibodies that neutralize SARS-CoV-2 are directed against the Spike protein. (forbes.com)
  • The native Gene 32 Protein from bacteriophage T4 (T4gp32) is a single-stranded DNA binding protein that is required for T4 DNA replication, recombination and repair. (qiagen.com)
  • The ability of T4 Gene 32 Protein to enhance the performance of several DNA synthesis-related activities is based on its essential function in the replication of bacteriophage T4. (qiagen.com)
  • This binding puts these ssDNA sequences into optimal conformations for interacting with DNA polymerases and other replication proteins. (qiagen.com)
  • however, a detailed understanding of effects of specific residues upon beta-turn stability and conformation is lacking. (rcsb.org)
  • Further structural modification was needed to achieve stability, whereas fusion proteins clamped using the gp41 fragments were stable on their own. (nature.com)
  • In this work we have studied the folding pathways for four pairs of homologous proteins from thermophilic and mesophilic organisms from two different structural classes (class a, all-alpha proteins and class d, alpha + beta proteins) using Monte Carlo simulations. (nih.gov)
  • We have observed that folding pathways of thermophilic and mesophilic proteins may be the same, partly the same, and different. (nih.gov)
  • And similarity or difference between the folding pathways of thermophilic and mesophilic proteins does not depend on the structural class to which these proteins belong. (nih.gov)
  • We have extended our analysis to investigate the energy landscape of thermophilic proteins. (berkeley.edu)
  • From experimentally validated target genes of these 86 miRNAs, pan-sensitive and pan-resistant genes with concordant mRNA and protein expression associated with in-vitro drug response to 19 NCCN-recommended breast cancer drugs were selected. (cdc.gov)
  • Some viruses possess an outer envelope, and the outer capsid is composed of major capsid protein (MCP), which appears to be highly conserved among the family and possesses surface binding sites interacting with the surfaces of host's cells [ 2 ]. (biomedcentral.com)
  • The same conformational change occurs when the spike protein is isolated or made by recombinant technology to produce a soluble subunit vaccine. (nature.com)
  • Chopping off the termini of proteins is a trick long-used to make soluble cytosolic proteins more amenable to crystallization. (the-scientist.com)
  • Structural analysis suggests that residues flanking the turn are a primary structural determinant of the conformation within the turn. (rcsb.org)
  • Genomic sequencing analysis of RSV in Arizona, USA, infections ( 2 ). (cdc.gov)
  • Additionally, BioID analysis indicated broad interaction between GATA4 and BET family of proteins, such as BRD4. (biomedcentral.com)
  • This hypothesis was borne out by analysis of other autotransporter sequences that also appear to have patterning based on spacing of hydrophobic clusters. (anl.gov)
  • Whole-exome sequencing was performed in eight individuals with HHCS from three different families, as well as one unaffected member from each family for trio analysis-a total of eleven individuals. (bvsalud.org)
  • METHODS: : The entire CDKN2A coding region and exon 2 of the CDK4 gene of an affected member of each of 52 families from southern Sweden with at least two cases of melanoma in first- or second-degree relatives were screened for mutations by use of polymerase chain reaction-single-strand conformation polymorphism analysis. (lu.se)
  • The DNA sequences of ospA , ospB , rrs-rrlA , and ospC ideal for deer and the small mammals that maintain the I. were determined for use in our phylogenetic analysis. (cdc.gov)
  • These fusion proteins are the basis of many subunit vaccines, a type in which a part of the pathogen being vaccinated against is injected to prompt the body to efficiently raise an immune response that can protect against the live virus. (nature.com)
  • The SARS-CoV-2 spike or fusion protein is a trimer: a group of three units, each consisting of a receptor-binding subunit, S1, and a subunit responsible for fusion, S2. (nature.com)
  • The UQ clamp that was developed consists of fragments of the fusion subunit of HIV's spike protein, gp41. (nature.com)
  • These proteins are synthesized in the bacterial cytoplasm and cross one membrane into the bacterial periplasm. (anl.gov)
  • Autotransporter proteins then remain in an unfolded state in the periplasm until they pass through the outer bacterial membrane, folding properly along the way. (anl.gov)
  • This highly specialized protein folding process has attracted the attention of a team of researchers who have used this bacterial system as a model to determine what allows these unique proteins to maintain their disordered state in the periplasm. (anl.gov)
  • showing the cavities through the channel, with the protein in grey surface, the pre-protein pore constrictions in red (SecY) or purple (SecA) mesh, and the SecY plug in red helix. (elifesciences.org)
  • Purity was assessed by comparing the aggregate mass of contaminant bands in the concentrated sample to the mass of the protein of interest band in the diluted sample. (qiagen.com)
  • Using a proteomic approach that coupled DNA affinity chromatography and mass spectrometry, the researchers identified a number of proteins that bind to HIV DNA, including IFI16. (genomeweb.com)
  • Our proposed voting mechanism based system provides a novel approach for in silico LE prediction prior to vaccine development, and it is especially powerful for analyzing antigen sequences with exclusive features between two clustered groups. (biomedcentral.com)
  • Folding pathways for proteins from both classes correlate with the calculated folding nuclei for these proteins. (nih.gov)
  • Amyloidosis results from the accumulation of pathogenic amyloids-most of which are aggregates of misfolded proteins-in a variety of tissues. (medscape.com)
  • An antibody ( Ab ), also known as an immunoglobulin ( Ig ), [1] is a large, Y-shaped protein used by the immune system to identify and neutralize foreign objects such as pathogenic bacteria and viruses . (wikipedia.org)
  • The system involves the study of what are termed autotransporter proteins, which pathogenic bacteria secrete as virulence factors for infection. (anl.gov)
  • We identified mutations within prefu- sion F-protein antigenic sites in both subtypes. (cdc.gov)
  • Many of the important antigenic sites on the spike protein become hidden or disappear because they literally change shape. (nature.com)
  • They discovered a new biosensing phenomenon called FLINC-Fluorescence fLuctuation INcrease by Contact-in which fluorescence fluctuations speed up when two fluorescent proteins are in close contact. (acs.org)
  • Zhang and coworkers discovered serendipitously that a fluorescent protein, Dronpa, significantly increases the rate of fluorescence fluctuations of another protein, TagRFP-T, when the two are in close proximity. (acs.org)
  • In addition to the native conformation, a protein sequence populates small fluctuations around the native state, partially unfolded forms and even the globally unfolded conformation. (berkeley.edu)
  • With 35,000 genes and hundreds of thousands of protein states to identify, correlate, and understand, it no longer suffices to rely on studies of one gene, gene product, or process at a time. (genomicglossaries.com)
  • Structurally based sequence alignment of DHFRs indicates the following levels of sequence identity and similarity for BsDHFR: 38 and 58% with Escherichia coli, 35 and 56% with Lactobacillus casei, and 23 and 40% with Thermotoga maritima, respectively. (duke.edu)
  • By coating these transiently exposed ssDNA sequences, gp32 also protects them from degradation by nucleases while they discharge their templating (and other) functions in association with the leading- and lagging-strand DNA polymerases. (qiagen.com)
  • Such non-native states on the energy landscape are thought to play a determining function in many cellular processes such as translocation, protein synthesis, degradation, signaling and allostery. (berkeley.edu)
  • The mRNA and protein expression of the 26 genes was associated with sensitivity or resistance to 18 NCCN-recommended drugs for treating breast cancer. (cdc.gov)
  • The pairs of residues on S1 and S2 predicted to be proximal in either closed or open conformations were mutated to Cys, and the effects of coupling were tested with patch-clamp and biochemically using Western blots. (usda.gov)
  • For both proteins, the oxygen-17 relaxation depended only very weakly on pD, showing that ionic residues do not perturb hydration water dynamics more than other surface residues. (lu.se)
  • Our use of different hydrogen exchange methods to study three different types of intermediates of E. coli ribonuclease H (the acid molten globule, the higher energy conformations of the native state and a kinetic folding intermediate) has been particularly powerful. (berkeley.edu)
  • In the wild-type protein these turns are of identical length, but exhibit different conformations. (rcsb.org)
  • Amyloid fibrils are protein polymers comprising identical monomer units (homopolymers). (medscape.com)
  • There were also some interesting data about different 'strains' of prions, all of which have identical sequences but different pathologies and host preferences. (bio.net)
  • In FLINC, one fluorescent protein, Dronpa, causes the fluorescence of another, TagRFP-T, to fluctuate when enzyme activity brings them into close proximity. (acs.org)
  • RSV) genomic sequences for 100 RSV-A and 27 RSV-B specimens collected during November 2022-April 2023 in Arizona, USA. (cdc.gov)
  • A) Five-week moving average of PCR-confirmed RSV detections in Arizona reported to the National Respiratory and tory-confirmed RSV cases increased beginning in Enteric Virus Surveillance System and RSV sequence counts by September 2022, peaked in mid-November 2022, and genotype obtained for specimens used in this study. (cdc.gov)
  • Chromosomal protein HMG-14. (unthsc.edu)
  • Dive into the research topics of 'Chromosomal protein HMG-14. (unthsc.edu)
  • A collection of different allotypes or allelic protein variants, a new type of protein library. (genomicglossaries.com)
  • Data indicated that ApoA-I variants exerted a cytotoxic effect in a time and cell-type-specific manner that seems to be due to protein accumulation in lysosomes. (lu.se)
  • While the binding did not to appear to affect protein conformations in solution, extended incubation of the amyloidogenic variants in the presence of different ECM components resulted in different aggregation propensity and aggregation patterns. (lu.se)
  • Figure, panel B). However, RSV vaccines are based on We tested 127 RSV-positive nasopharyngeal the prefusion conformation of the F protein. (cdc.gov)
  • 60 years of age, A and RSV-B reference sequences (GISAID acces- both based on the RSV prefusion F protein. (cdc.gov)
  • protein interaction has still not emerged. (lu.se)
  • Understanding the sequence determinants of the energy landscape is therefore fundamental to the biological process that proteins carry out as well as protein folding itself. (berkeley.edu)
  • The authors believe their work will provide important information toward understanding basic questions of protein folding and tests long-held theories about how this remarkable biological process works. (anl.gov)
  • In prokaryotes, SecYEG associates with the motor ATPase SecA to carry out translocation for pre-protein secretion. (elifesciences.org)
  • This paper explores how the protein folding process per se may be exploited to drive protein translocation. (elifesciences.org)
  • The protein must then fold during or after the translocation process. (elifesciences.org)
  • These proteins either arise from proteins expressed by cells at the deposition site (localized), or they precipitate systemically after production at a local site (systemic). (medscape.com)
  • However, there is no way of knowing in advance which species or expression method is going to yield a well-behaved protein. (the-scientist.com)