GeneGenesSangerIntronsMutationsNucleotideWhole-genomeExomePrimersGeneticsPutativelyGenomic sequenceGenome sequencesAmino acid sequencesVariantsIntronAmplifyProteinsPromoterTBCELociTranscriptsAnalysisMRNAGenomesMethodsFindingsMutation detectionUpstreamHumansMotifCaptureNext-generationMicroarrayFoundResultsGeneticClinicalDataStrandSpecificCDNAIdentifyHumanReactionSNPs
Gene35
- I want to download the sequences of all the exons for each human gene. (biostars.org)
- my bed file is not in bed12 and each exon is in a separate row (though the name column of the bed file is the gene ID). (biostars.org)
- SimpleChIP ® Human DNAJB9 Exon 1 Primers contain a mix of forward and reverse PCR primers that are specific to exon 1 of the human DnaJ homolog subfamily B member 9 gene. (cellsignal.com)
- Spliced leader sequences are short sequences of non coding RNA, not found within a gene itself, that are attached to the 5' end of all, or a portion of, mRNAs transcribed in an organism. (wikipedia.org)
- Targeted Induced Local Lesions in Genomes (TILLING) is a reverse genetics approach to identify novel sequence variation in genomes, with the aims of investigating gene function and/or developing useful alleles for breeding. (pubchase.com)
- We included identification of copy number variations (CNVs) by whole exome sequencing (WES) using the CNV calling method ExomeDepth to detect gene alterations for which routine Sanger sequencing analysis is not suitable, such as large heterozygous deletions. (sunderland.ac.uk)
- We found heterozygous single- or multi-exon deletions in IL7R, a known disease gene for autosomal recessive T-B+NK+ SCID, in four families (seven patients). (sunderland.ac.uk)
- Comparatively short sequences (several hundred to several thousand base pairs, depending on thespecies) upstream or downstream of the transcription start site often play a major role in the regulation of gene expression. (biomedcentral.com)
- Subsequent screening for structural variants in candidate genes located in the same regions identified a homozygous deletion that includes exons 17 to 23 of the integrin beta 4 ( ITGB4 ), a gene that was previously associated with the same defect in humans. (biomedcentral.com)
- CNV analysis should routinely be applied in targeted NGS, and mutations in non-coding exons give reason to systematically include 5′-UTRs in disease gene or exome panels. (aku.edu)
- Identification of pathogenic variants in the Brazilian cohort with Familial hypercholesterolemia using exon-targeted gene sequencing. (bvsalud.org)
- Exon 8 of the mouse α 6 subunit gene was disrupted by homologous recombination. (jneurosci.org)
- Methods A next-generation sequencing (NGS) panel was created for the human TRPV1 gene and in addition, for the leukotriene receptors BLT1 and BLT2 recently described to modulate TRPV1 mediated sensitisation processes rendering the coding genes LTB4R and LTB4R2 important co-players in pharmacogenetic approaches involving TRPV1. (researchgate.net)
- However, researchers have found that DNA variations outside the exons can affect gene activity and protein production and lead to genetic disorders--variations that whole exome sequencing would miss. (medlineplus.gov)
- While many more genetic changes can be identified with whole exome and whole genome sequencing than with select gene sequencing, the significance of much of this information is unknown. (medlineplus.gov)
- Zheng's team now reports the central piece of the mechanism involved is a small piece of genetic sequence in Bak1, a pro-apoptotic gene whose activation leads to apoptosis. (prohealth.com)
- Bak1 expression is turned off when this small piece of genetic sequence, termed microexon, is spliced in the final Bak1 gene product. (prohealth.com)
- Let's consider a DNA sequence that contains a gene of interest. (genome.gov)
- When examining an unknown DNA sequence, one indication that it may be part of a gene is the presence of an open reading frame or ORF. (genome.gov)
- The presence of an ORF doesn't guarantee that the DNA sequence is part of a gene. (genome.gov)
- Likewise, codons for methionine do not always mark the start of a gene sequence. (genome.gov)
- Here we report a silent variant found in exon 2 in the F8 gene in a 47-year-old patient with a previous von Willebrand disease (VWD) type 1 diagnosis. (lu.se)
- A gene encoding a cotton ADP-glucose pyrophosphorylase (ADPGp) gene was isolated, sequenced and characterized. (usda.gov)
- The gene had a structure consisting of 8 introns and 9 exons, similar to other plant AGPGp genes. (usda.gov)
- The protein that the gene encoded also had many features similar to other plant ADPGp including a variable amino end, conserved catalytic sites and conserved amino acid sequences that control enzyme activity. (usda.gov)
- Analysis of the promoter region failed to identify a complete basal promoter but did identify sequences indicating that expression of this gene may respond to various phytohomones and light regimes. (usda.gov)
- A cotton gene encoding ADPGp small subunit was isolated and sequenced. (usda.gov)
- In silico sequence analysis of the promoter region of the gene identified motifs associated with light regulation, circadian response, endosperm expression, ethylene response and gibberellic acid response. (usda.gov)
- We report 8 patients from 7 Jordanian families, 6 of whom underwent genetic testing and were found to have a 12 bp (155-166 del) deletion within the tubulin-specific chaperone E ( TBCE gene) in exon 3 at 1q42-43. (who.int)
- These primers amplify approximately 495 bp of exons and introns at the 5' end of the β-tubulin gene. (cdc.gov)
- All affected persons had homozygous deletion of 12 bp (155-166del) in exon 3 of the TBCE gene. (who.int)
- Exons 2 (first coding exon), 3 (second cod- and Kuwait: the incidence in Saudi Ara- ing exon) and 12 of the TBCE gene were bia varies from 1:40 000 to 1:100 000 live chosen for the initial screening as they were births [ 6 ]. (who.int)
- The OCRL gene is located on Xq25-q26 and consists of 24 exons occupying 52kb. (medscape.com)
- Most mutations found are of missense type and located in the central region of the gene (exons 5 to 8). (lu.se)
- A 133 bp fragment, spanning exon 11 of the APC gene was amplified, and Rsal digestion of the PCR product defined the alleles as either homozygous 133 bp (Rsa(-/-)) or 87 and 46 bp (Rsa(+/+)) fragments, and heterozygous (Rsa(+/-)) exhibiting the three fragments. (who.int)
Genes25
- Because longer genes encode longer BPRPs, we investigated the possibility of exon sequencing BPRP alleles encoded by PRB1 and PRB2. (umich.edu)
- Of the 16 genes sequenced, 2 had a crossover between PRB1 and PRB2 and 4 had stop mutations. (umich.edu)
- This prevents bias associated with restriction site sequences that will be missing from some genes, as is seen in SAGE. (wikipedia.org)
- An oligonucleotide-based enrichment array covering ~2 Mbp of wheat coding sequence was used to carry out exon capture and sequencing on three mutagenised lines of wheat containing previously-identified mutations in the TaGA20ox1 homoeologous genes. (pubchase.com)
- To uncover "hidden mutations" such as copy number variations (CNVs) and mutations in non-coding regions, we extended the use of NGS data by quantitative readout for the exons of 55 RP and LCA genes in 126 patients, and by including non-coding 5′ exons. (aku.edu)
- We further explored the cancer-promoting mechanism of target genes in ESCC by sequencing of the genes' exons. (hindawi.com)
- Genes also include regulatory sequences. (livescience.com)
- Although scientists don't fully understand their function, regulatory sequences help turn genes on. (livescience.com)
- The NGS workflow was based on a custom AmpliSeq™ panel and designed for sequencing of human genes on an Ion PGM™ Sequencer. (researchgate.net)
- A cohort of 80 healthy subjects of Western European descent was screened to evaluate and validate the detection of exomic sequences of the coding genes with 25 base pair exon padding. (researchgate.net)
- This identified approximately 140 chromosome loci where nucleotides deviated from the reference sequence GRCh37 hg19 comprising the three genes TRPV1, LTB4R and LTB4R2. (researchgate.net)
- It is suitable for large-scale sequencing of TRPV1 and functionally related genes. (researchgate.net)
- Results from RNA sequencing revealed that AEG‑1 modulated neuronal morphology and development by regulating the expression of numerous genes, such as ubiquitin C, C‑X‑C motif chemokine ligand 1, MMP9, Notch1, neuropilin 1 and ephrin type‑A receptor 4. (spandidos-publications.com)
- GeneReviews compares whole genome sequencing, whole exome sequencing, and the sequencing of a selection of individual genes , particularly their use in the diagnosis of genetic conditions. (medlineplus.gov)
- One of the most important aspects of bioinformatics is identifying genes within a long DNA sequence. (genome.gov)
- Bioinformatics allows scientists to make educated guesses about where genes are located simply by analyzing sequence data using a computer (in silico). (genome.gov)
- Nevertheless, searching for ORFs identifies regions of the DNA sequence that might be parts of genes. (genome.gov)
- Most eukaryotic genes take the form of alternating exons and introns. (genome.gov)
- Genes are also characterized by specific control sequences that are recognized by enzymes involved with transcription and translation. (genome.gov)
- Emory Genetics Laboratory is now offering a clinical exome sequencing test with enhanced coverage of around 4,600 disease-associated genes that the lab hopes will bump up the diagnostic yield of exome testing, currently hovering between 25 and 30 percent. (genomeweb.com)
- At the Future of Genomic Medicine conference in San Diego, Calif., earlier this month, a number of clinical laboratory directors spoke of the problem of exome sequencing missing important disease-related genes, including even in protein-coding regions. (genomeweb.com)
- Due to these issues, EGL, CHOP, and LMM have had an ongoing collaboration to identify and curate a list of medically relevant genes, define precisely which of those exons were being missed by commercial capture kits and why, and develop approaches to target those areas. (genomeweb.com)
- This document describes some of the target genes and primers that can be used for DNA sequence-based identification of fungi and the PCR conditions with which to use those primers. (cdc.gov)
- In BASE, reporters also refer to Affymetrix Probeset ID but reporters can be used to describe genes, transcripts, exons or any other sequence entity of biological relevance. (lu.se)
- Reporters are used to represent genes, transcripts, exons and therefore come in their thousands. (lu.se)
Sanger6
- They should be considered if Sanger sequencing fails to detect homozygous or compound heterozygous IL7R SNVs or INDELs. (sunderland.ac.uk)
- RNA Sanger sequencing analysis was performed in AEG‑1‑deficient HT22 cells and wild‑type HT22 cells without knockout (KO). (spandidos-publications.com)
- The original sequencing technology, called Sanger sequencing (named after the scientist who developed it, Frederick Sanger), was a breakthrough that helped scientists determine the human genetic code, but it is time-consuming and expensive. (medlineplus.gov)
- The Sanger method has been automated to make it faster and is still used in laboratories today to sequence short pieces of DNA, but it would take years to sequence all of a person's DNA (known as the person's genome). (medlineplus.gov)
- Sanger sequencing of the patient's cDNA after nested polymerase chain reaction showed that the patient had both a normal transcript containing. (lu.se)
- For those areas, Emory supplements coverage with spike-in probes from Integrated DNA Technologies, or by doing Sanger sequencing to fill in those areas. (genomeweb.com)
Introns2
- RBM10 recognizes a diverse set of RNA motifs in introns and exons and regulates alternative splicing. (crick.ac.uk)
- Longer "nonsense" DNA, known as introns, flank the exons. (livescience.com)
Mutations6
- The proposed role of HRPT2 as a tumor suppressor was supported by mutation screening in 48 parathyroid adenomas with cystic features, which identified three somatic inactivating mutations, all located in exon 1. (nih.gov)
- Mutations of non-coding exon 1 of EYS revealed its contribution to disease. (aku.edu)
- Because most known mutations that cause disease occur in exons, whole exome sequencing is thought to be an efficient method to identify possible disease-causing mutations. (medlineplus.gov)
- In Kuwait, no precise data are the only exons where mutations have been available, although some reports have been previously reported [ 5 ]. (who.int)
- Sequence analysis detects mutations in 95% of males and 95% of female carriers. (medscape.com)
- Based on the TBX5, GATA4, and HEY2, often simultaneously incidence of somatic mutations described in the within the same patient.9-13 The observation of two previously published reports, our study was adequately or more somatic sequence variants in the same powered to replicate the previous studies. (cdc.gov)
Nucleotide3
- Nucleotide sequences contained numerous single nucleotide polymorphisms (SNPs), including amino acid to stop codon conversions and crossover events that deleted portions of PRB1 and/or PRB2. (umich.edu)
- Additional evaluation of HLA-A promoter and exon 1 sequences did not detect any unexpected single nucleotide polymorphisms that could suggest differential allelic expression. (cdc.gov)
- Nucleotide sequences located at the ends of EXONS and recognized in pre-messenger RNA by SPLICEOSOMES. (bvsalud.org)
Whole-genome7
- Therefore, to address the growing volumes of available genomic sequence, a number of algorithms that identify putative cis-regulatory modules and transcription factor binding sites using evolutionary comparisons, whole-genome data, and known descriptions of transcription factor binding sites, have been successfully developed. (biomedcentral.com)
- 1 Mb) were identified after analysing the whole-genome sequencing (WGS) data from the only case available for DNA sampling at the beginning of the study. (biomedcentral.com)
- What are whole exome sequencing and whole genome sequencing? (medlineplus.gov)
- Another method, called whole genome sequencing, determines the order of all the nucleotides in an individual's DNA and can determine variations in any part of the genome. (medlineplus.gov)
- In addition to being used in the clinic, whole exome and whole genome sequencing are valuable methods for researchers. (medlineplus.gov)
- The American College of Medical Genetics and Genomics has laid out their policies regarding whole exome and whole genome sequencing , including when these methods should be used, what results may arise, and what the results might indicate. (medlineplus.gov)
- The Medical College of Wisconsin's Howard Jacob said that of 25 patients subjected to both exome and whole-genome sequencing, exome sequencing missed 212 actionable variants, while whole-genome sequencing missed three. (genomeweb.com)
Exome8
- Methods: All exons of PRB1 and PRB2 from 8 healthy adults were sequenced using an Illumina HiSeq 2000 (100 bp paired sequencing readouts with TruSeq™ Exome Enrichment Kit). (umich.edu)
- Identification of Heterozygous Single- and Multi-exon Deletions in IL7R by Whole Exome Sequencing. (sunderland.ac.uk)
- Agilent provides a whole exome sequencing solution for genetics labs. (agilent.com)
- Together, all the exons in a genome are known as the exome, and the method of sequencing them is known as whole exome sequencing. (medlineplus.gov)
- Continued study of exome and genome sequences can help determine whether new genetic variations are associated with health conditions, which will aid disease diagnosis in the future. (medlineplus.gov)
- The Mount Sinai School of Medicine Genomics Core Facility describes the techniques used in whole exome sequencing . (medlineplus.gov)
- While clinical exome sequencing has had a number of high-profile successes in diagnosing children's unexplained disease and in some cases leading to dramatic clinical improvement in their conditions, the diagnostic rate has stalled at around 25 percent, in part because current exome capture kits do not cover the entire exome. (genomeweb.com)
- Off-the-shelf kits cover up to around 92 percent of the exome, but between 11 percent and 20 percent of the known clinically relevant exons are either poorly covered or not covered at all. (genomeweb.com)
Primers4
- SimpleChIP ® Human DNAJB9 Exon 1 Primers were tested on DNA isolated from cross-linked cells using the SimpleChIP ® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. (cellsignal.com)
- PCR product melting curves were obtained for real-time PCR reactions performed using SimpleChIP ® Human DNAJB9 Exon 1 Primers. (cellsignal.com)
- The cDNA produced from the mRNA is labeled using primers homologous to the spliced leader sequences of the organism. (wikipedia.org)
- Exons 2 and 3 of the HLA-B locus were amplified with locus specific primers and the amplified DNA was arrayed onto seven replicate nylon membranes and immobilized by UV cross-linking. (cdc.gov)
Genetics1
- Determining the order of DNA building blocks (nucleotides) in an individual's genetic code, called DNA sequencing, has advanced the study of genetics and is one technique used to test for genetic disorders. (medlineplus.gov)
Putatively1
- The results of this research rule out a possible role of putatively regulatory sequences for transacting elements, an activator and a repressor, because the expressions of c-myc were not affected by the exogenous elements of exon 1 or upstream sequence in NIH3T3 fibroblast cells. (e-crt.org)
Genomic sequence1
- While the genomic sequence of DcAOX2a was previously described, we characterize here the complete genomic sequence of DcAOX1 . (frontiersin.org)
Genome sequences1
- Sr61 orthologs are absent from current Thinopyrum elongatum and wheat pan genome sequences, contrasting with Sr26 where homologues are present. (nature.com)
Amino acid sequences1
- The migration of ATP7A appears to involve amino acid sequences in the carboxyl terminus, utilizing both clathrin-dependent and clathrin-independent endocytosis. (medscape.com)
Variants10
- Sequence reads were assembled to the human reference genome using Burrows-Wheeler Aligner software (BWA) and the Genome Analysis Tool Kit (GATK) which identifies genomic variants. (umich.edu)
- The identification of alternative splice variants, and possibly the relative quantities, containing a trans-spliced leader sequence is therefore possible. (wikipedia.org)
- Histone variants differ from the canonical histones in their amino acid sequence and have specific and fundamental functions that cannot be performed by canonical histones. (nature.com)
- Sequencing technology has been used to detect rare variants in many studies [ 7 - 10 ], with rare variants defined as 1%-5% frequency. (hindawi.com)
- One of the challenges of genetic testing in patients with hemophilia A is the interpretation of sequence variants. (lu.se)
- The Web site "www.artichokegenome.unito.it" hosts all the available genomic sequences, together with their structural/functional annotations and project information are presented to users via the open-source tool JBrowse, allowing the analysis of collinearity and the discovery of genomic variants, thus representing a one-stop resource for Cynara cardunculus genomics. (researchgate.net)
- Dr Michael Mitchell, Division of Cardiothoracic Surgery, defects (ASD, n = 13), ventricular septal defects (VSD, NKX2-5 sequence variants were found in .95% of Department of Surgery, Medical n = 5), and atrioventricular canal defects (AVCD, n = 10). (cdc.gov)
- Accepted 1 August 2008 the same methodology described in the previously that somatic sequence variants occur with high published reports. (cdc.gov)
- Two synonymous germline in a majority of these patients.10 13 The same sequence variants were identified in two separate investigators also found somatic sequence variants patients. (cdc.gov)
- The somatic NKX2-5 sequence variants were defects. (cdc.gov)
Intron1
- The intron sequences do not code for amino acids and contain internal stop codons. (genome.gov)
Amplify1
- Using Oligo software, Kuwait identified as KCS type 1 showed version 3.4, 3 polymerase chain reaction that they fulfilled the criteria for SSS, and (PCR) primer sets to amplify exons 2, 3, and might be considered as such. (who.int)
Proteins1
- With next-generation sequencing, it is now feasible to sequence large amounts of DNA, for instance all the pieces of an individual's DNA that provide instructions for making proteins. (medlineplus.gov)
Promoter1
- Sr26 and Sr61 are each validated by transgenic complementation using endogenous and/or heterologous promoter sequences. (nature.com)
TBCE1
- les analyses génétiques réalisées sur six d'entre eux ont révélé une délétion de 12 bp (155-166 del) dans l'exon 3 localisé en 1q42-43 dans le gène TBCE codant la protéine chaperon E spécifique de la tubuline. (who.int)
Loci1
- HLA-B27 testing was performed on these samples using a polymerase chain reaction (PCR) to specifically replicate the DNA sequences encoding the HLA loci of interest. (cdc.gov)
Transcripts2
- The issue you have with more sequences and exons is most likely due to alternative transcripts. (biostars.org)
- Unlike SAGE, detection and purification of transcripts from the 5' end of the messenger RNA require the presence of a trans-spliced leader sequence. (wikipedia.org)
Analysis7
- Two alternate techniques have been described that allow for 5' tag analysis in organisms that do not have trans-spliced leader sequences. (wikipedia.org)
- cDNA analysis showed that the deleterious effect of the latter mutation was skirted by deleting the premature termination codon containing exon 30. (nih.gov)
- Sequence chromatograms and segregation analysis of the p.S205X GRK1 variant. (molvis.org)
- StuI restriction analysis of the 552 bp PCR products of exon 1 in all family members. (molvis.org)
- Trio sequencing, which Hegde said the lab prefers to do because it facilitates analysis, will cost $6,700. (genomeweb.com)
- mean age 58 years) using single stranded conformation polymorphism and sequence analysis. (lu.se)
- PCR (polymerase chain reaction) technique has achieved increased importance for post-mortem DNA analysis in forensic cases 8 because of the millions of copies amplified from one specific sequence of DNA. (bvsalud.org)
MRNA2
- Sequence of chicken ovalbumin mRNA. (wikidata.org)
- CAGE utilizes biotinylated cap-trapper technology to maintain mRNA signal long enough to create and select full length cDNAs, which have adapter sequences ligated on the 5' end. (wikipedia.org)
Genomes1
- This is an actively progressing area, as more and more sequenced genomes appear. (biomedcentral.com)
Methods2
- We aimed to achieve a retrospective molecular diagnosis by applying state-of-the-art genomic sequencing methods to past patients with T-B+NK+ severe combined immunodeficiency (SCID). (sunderland.ac.uk)
- both methods rely on new technologies that allow rapid sequencing of large amounts of DNA. (medlineplus.gov)
Findings1
- Together, these findings explain RBM10's broad RNA specificity and suggest that RBM10 functions as a splicing regulator using two RNA-binding units with different specificities to promote exon skipping. (crick.ac.uk)
Mutation detection2
- This demonstrates the feasibility of using exon capture for genome re-sequencing as a method of mutation detection in polyploid wheat, but accurate mutation calling will require an improved genomic reference with more comprehensive coverage of homoeologues. (pubchase.com)
- Conclusions Results suggested that the NGS approach based on AmpliSeq™ libraries and Ion Personal Genome Machine (PGM) sequencing is a highly efficient mutation detection method. (researchgate.net)
Upstream2
- This research was designed to investigate the possible effect of exon 1 and its upstream sequences on c-myc expression. (e-crt.org)
- The transcriptional activities of exogenous plasmids and endogenous c-myc were studied for their expression after NIH3T3 cells were transiently transfected with plasmids containing exon 1 and upstream sequences of mouse c-myc that are putativelv regulatory sequences. (e-crt.org)
Humans1
- The team first compared neural tissues with non-neural tissues in both humans and mice to identify neural-specific exons. (prohealth.com)
Motif3
- We show that a two-domain RRM1-ZnF unit recognizes a GGA-centered motif enriched in RBM10 exonic sites with high affinity and specificity and test that the interaction with these exonic sequences promotes exon skipping. (crick.ac.uk)
- The potential application of our method includes annotation of new genomic sequences and motif discovery. (biomedcentral.com)
- Further analyses restricted to female GBM cases and controls revealed a second association with a specific HLA-B sequence motif corresponding to Bw4-80Ile (odds ratio = 2.71, p = 0.02). (cdc.gov)
Capture3
- Mutation Scanning in Wheat by Exon Capture and Next-Generation Sequencing. (pubchase.com)
- We performed a pilot-scale evaluation of TILLING in wheat by next-generation sequencing through exon capture. (pubchase.com)
- Either the kits have not been designed to capture those exons in the first place, or there is lost coverage due to sequence complexity even though the capture kit attempts to target them. (genomeweb.com)
Next-generation4
- Recently, targeted next-generation sequencing (NGS) has proven useful to overcome this limitation. (aku.edu)
- Next-generation sequencing (NGS) technology has provided researchers with opportunities to study the genome in unprecedented detail. (hindawi.com)
- These approaches are known as next-generation sequencing (or next-gen sequencing). (medlineplus.gov)
- Next-generation sequencing has sped up the process (taking only days to weeks to sequence a human genome) while reducing the cost. (medlineplus.gov)
Microarray1
Found3
- Some species contain only one spliced leader sequence found on all mRNAs. (wikipedia.org)
- We found that pooled sequencing data can introduce high levels of relative error rate (defined as error rate divided by targeted allele frequency) and that the error rate is more severe for low minor allele frequency SNPs than for high minor allele frequency SNPs. (hindawi.com)
- DNA for PCR and sequencing was extracted from 1 found in almost all disease-associated STEC strains. (cdc.gov)
Results2
- Results The amplicons covered approximately 97% of the target sequence. (researchgate.net)
- Results: One non-synonymous germline sequence variant iants as well as multiple haplotypes were observed was identified in one patient. (cdc.gov)
Genetic1
- The expression of Bak1 is turned off when a small piece of genetic sequence, called microexon, is spliced. (prohealth.com)
Clinical1
- Hegde, executive director of EGL, told Clinical Sequencing News that the laboratory began offering the Medical EmExome internally in January and is now expanding the service broadly. (genomeweb.com)
Data1
- In this study, we designed a rigorous simulation model to test the practicability of estimating allele frequency from pooled sequencing data. (hindawi.com)
Strand3
- A DNA sequence is a specific lineup of chemical base pairs along its strand. (livescience.com)
- The DNA strand that codes for the protein is called the sense strand because its sequence reads the same as that of the messenger RNA. (genome.gov)
- The program transcribes each DNA strand into its complementary RNA sequence and then translates the RNA sequence into an amino acid sequence. (genome.gov)
Specific4
- However, such an approach offers little information as to the specific function of the conserved sequences. (biomedcentral.com)
- One pathway, which has been extensively studied in yeast, is mainly guided by chromatin structure and the other, analyzed in detail in mice, is driven by the sequence-specific DNA-binding PR domain-containing protein 9 (PRDM9). (springer.com)
- There are two main reasons for no or low coverage of specific exons, Hegde said. (genomeweb.com)
- The cross-linked sample DNA was hybridized with sequence-specific oligonucleotide probes to identify HLA-B27 allele sequences. (cdc.gov)
CDNA2
- I also tried to download coding sequence and cDNA, but the length of both sequences is not consistent with 'official' BRCA2 length! (biostars.org)
- Both use their adapter sequence to prime from after the cDNA is created. (wikipedia.org)
Identify1
- The first step was to "identify the exons without probes. (genomeweb.com)
Human2
- pathogens with the closely related human pathogen, en- teropathogenic E. coli ( 4 , 5 ). (cdc.gov)
- An illustration of the decline in the cost of DNA sequencing , including that caused by the introduction of new technologies, is provided by the National Human Genome Research Institute. (medlineplus.gov)
Reaction1
- They are joined during the RNA SPLICING reaction, forming the junctions between exons. (bvsalud.org)
SNPs1
- After testing different mapping algorithms and settings, candidate SNPs were identified by mapping to the IWGSC wheat Chromosome Survey Sequences. (pubchase.com)