Sequence cloning molecular. Medical search. Frequent questions

The rat G-protein-coupled receptor kinase 6 (GRK6) cDNA was cloned from rat brain tissue by a combination of reverse-transcription polymerase chain reactions (RT-PCR), based on homology to the cloned human GRK6, and rapid amplification of cDNA ends (RACE-PCR). (nih.gov)
In this article, we report cloning of the rat apelin receptor cDNA. (nih.gov)
The sequence of the alpha 2 beta cDNA shows the presence of a single Arg residue between Gln 142 of the alpha 2 chain and Ileu 1 of the beta chain. (rti.org)
To aid in the examination of this phenomenon, the cDNA sequences encoding estrogen receptor-alpha (esr1), estrogen receptor-beta-a (esr2a) and ovarian aromatase (cyp19a1a) for the yellow perch were obtained. (usda.gov)
3] "Cloning and nucleotide sequencing of transthyretin (prealbumin) cDNA from rat choroid plexus and liver. (tcdb.org)

Scholars@Duke publication: Molecular cloning and analysis of functional envelope genes from human immunodeficiency virus type 1 sequence subtypes A through G. The WHO and NIAID Networks for HIV Isolation and Characterization. (duke.edu)
Molecular and functional characterization of a SCD 1b from European sea bass (Dicentrarchus labrax L. (researchgate.net)

Whole genome sequencing (WGS) is increasingly used for pathogen identification and surveillance. (eurosurveillance.org)
They were able to sequence the whole genome and confirm that the bacteria did not carry genetic markers for antibiotic resistance or evidence of genetic engineering in only 8.5 hours. (cdc.gov)
Genome-Wide Association Study of HIV Whole Genome Sequences Validated using Drug Resistance. (cdc.gov)
Bioinformatics tools and databases for whole genome sequence analysis of Mycobacterium tuberculosis. (cdc.gov)
Whole genome sequencing identifies in vivo acquisition of a blaCTX-M-27-encoding IncFII transmissible plasmid as the cause of ceftriaxone treatment failure for an invasive Salmonella enterica serovar Typhimurium infection. (cdc.gov)

Find out more at Oxford Genetics - Sigma's partner for cloning and expression vectors for molecular biology and synthetic biology applications. (sigmaaldrich.com)

Subsequent cloning and sequencing of gcsap fragments obtained from PCR with genomic DNA revealed a 73 bp intron beginning at nt 728. (microbiologyresearch.org)
Established in the mid 1970's, New England Biolabs, Inc. (NEB) is the industry leader in the discovery and production of enzymes for molecular biology applications and now offers the largest selection of recombinant and native enzymes for genomic research. (prnewswire.com)
It is well equipped with molecular and cellular biology technologies to conduct virus isolation, genomic sequencing, and cloning, through which determines the common virus strains circulating in the country. (who.int)
and (i i) to develop (epi)genomic powerful molecular and/or cell biology and close col aboration to create synergies methodologies, profiling strategies, and functional genomics tools, recent progress and better exploit and further expand bioinformatics tools and resources that in understanding of the cancer (epi) unique research tools and expertise. (who.int)

We obtained a clone of 2817 bp with an open reading frame of 1731 bp encoding for a protein of 576 amino acids that is 96.7% identical and 97.9% similar to its human counterpart. (nih.gov)
Comparison of the deduced amino acid sequences of the giant panda with those of these other species revealed that the RPS14 of giant panda is highly homologous with those of B. taurus, R. norvegicus and D. rerio (85.99, 99.34 and 99.34%, respectively), and is 100% identical with the others. (geneticsmr.com)
Analysis of their deduced amino acid sequences revealed several important differences from prototypic subtype B strains, including changes in the number and distribution of cysteine residues, substantial length differences in hypervariable regions, and premature truncations in the gp41 domain. (duke.edu)
One of these, derived from a patient with rapid CD4 cell decline, contained an amino acid substitution in a highly conserved endocytosis signal (Y721C), as mediated virus entry with very poor efficiency, although they did not contain sequence changes predicted to alter protein function. (duke.edu)
a ) Amino acid sequence comparison of human and mouse podocin. (jci.org)
Based on the deduced sequence, the mature enzyme contains 329 amino acids and shows approximately 40% identity to other fungal aspartic proteinases. (microbiologyresearch.org)
Amino acid sequence of rhizopuspepsin isozyme pl5. (microbiologyresearch.org)
With a few minor exceptions, the DNA sequence fits the previously published amino acid sequences. (rti.org)
The nucleotide and deduced amino acid sequences of preprochensinin-1 were not similar to any known amphibian skin defensive peptides. (bioone.org)
Four bioactive peptides were chemically synthesized according to the deduced amino acid sequences of six prepropeptides from R. chensinensis skin, and their antimicrobial, cytotoxic, and haemolytic properties were evaluated. (bioone.org)
The Open reading frame (ORF) of the AnpeNPV consists of 738 nucleotides encoding 245 amino acids with molecular masses of 29 kDa. (scirp.org)
Prediction of protein antigenic determinants from amino acid sequences. (scirp.org)

E. A. van Strien, D. Zuidema, R.W. Goldbach, Vlak, J. M. (1992) Nucleotide sequence and transcriptional analysis of the polyhedrin gene of Spodoptera exigua nuclear polyhedrosis vi-rus. (scirp.org)
Molecular cloning, nucleotide sequence, and gene expression in liver and brain. (tcdb.org)

Using a combination of next-generation sequencing, bioinformatics, and molecular cloning, CDC can identify the functions of the individual antibodies and what part of the influenza virus they target. (cdc.gov)

PCR (polymerase chain reaction) technique has achieved increased importance for post-mortem DNA analysis in forensic cases 8 because of the millions of copies amplified from one specific sequence of DNA. (bvsalud.org)

Monitoring the long-term molecular epidemiology of the pneumococcus and detection of potential 'vaccine escape' strains. (jcvi.org)
Thus, the molecular approach was applied for the first time, in this country, to investigate the molecular epidemiology of malaria. (biomedcentral.com)
Our objective was to characterize the molecular epidemiology and AMR mechanisms of this pathogen. (who.int)

We have sequenced the cloned DNA and have found no homology between the Na + /glucose co-transporter and either the mammalian facilitated glucose carrier or the bacterial sugar transport proteins. (nature.com)
In contrast, the major satellite DNA sequences that had a low sequence homology are present only in a few species. (edu.au)

The result of all these early efforts can be seen in the fact that molecular diagnostic tests are now on the market for most, if not all, of these genetic diseases. (genengnews.com)
Fatty acid desaturation is a highly complex and regulated process involving different molecular and genetic actors. (researchgate.net)
In addition, malaria strains, genetic polymorphism and the molecular phylogeny of Yemen Plasmodium isolates will be studied. (biomedcentral.com)
Modern genetic techniques have helped us hugely in characterizing these disorders and providing a molecular diagnosis in the face of nonspecific clinical data. (medscape.com)
Gene technology: basic and applied molecular genetic methods. (lu.se)
The lab consists of molecular tools that can be used for genetic modification of neurons. (lu.se)

Scholars@Duke publication: Molecular cloning of the gene sequences of a major apoprotein in avian very low density lipoproteins. (duke.edu)

Differentiation of Schistosoma haematobium from related species using cloned ribosomal RNA gene probes. (ajtmh.org)
Some of the enzymes which generate aromatic radicals that break down most prominent bacterial strains found were isolated and tax- the complex linkages present in lignin to compounds of lower onomically identified using 16S ribosomal RNA (rRNA) se- molecular weight. (lu.se)

Phylogenetic analyses of full-length env sequences indicated that the panel included members of all major sequence subtypes of HIV-1 group M (clades A to G), as well as an intersubtype recombinant (F/B) from an infected individual in Brazil. (duke.edu)
From the 86 positive blood samples, 13 Plasmodium falciparum and 4 Plasmodium vivax were selected and underwent cloning and, subsequently, sequencing and the sequences were subjected to phylogenetic analysis using the neighbor-joining and maximum parsimony methods. (biomedcentral.com)

These sequences are valuable molecular tools that can be used in future studies investigating estrogen mechanisms and actions, such as SSD, in yellow perch. (usda.gov)
The work package focusing on molecular studies generated new evidence about the changes effected by antibiotic therapy on commensal organisms or opportunistic pathogens in the oropharyngeal, nasal and gastro-intestinal flora and study AMR mechanisms and the dissemination of successful clones of fluoroquinolone-resistant, carbapenem-resistant or extended-spectrum beta-lactamase harboring Gram-negative bacteria, MRSA and fluoroquinolone-resistant viridans streptococci. (europa.eu)
My research focuses on the molecular mechanisms and biomarkers of rejection after allograft transplantation. (stanford.edu)
Molecular mechanisms for regulation of gene expression at different levels: remodeling of chromatin, initiation of transcription, nuclear transport and signalling, and RNA interference. (lu.se)

Therefore, multi-locus sequence typing (MLST)-defined clones could conceivably include strains from multiple PspA clades. (jcvi.org)

Subsequently a stable CHO cell line expressing the receptor fused at its C-terminal part with the enhanced green fluorescent protein (EGFP) was established, allowing to verify its cell surface distribution and to determine the affinity of various apelin and angiotensin fragments on the cloned receptor. (nih.gov)
The novel, proprietary DNA polymerase in the NEBuilder HiFi enzyme mix results in extremely accurate fragment assemblies and cloning junctions, and can assemble DNA fragments ranging from 100 bp to at least 19 kb. (prnewswire.com)
Scientists using fragments designed using other seamless cloning methods can easily assemble these with NEBuilder HiFi, due to compatibility of the fragments. (prnewswire.com)

Here we describe cloning of this co-transporter by a method new to membrane proteins. (nature.com)

In an effort to gain more understanding into the yellow perch E2 stimulated SSD, the cDNAs for genes that encode for hormones that are involved with the endocrine growth axis (GH, PRL, SL and IGF-I) have been recently cloned and characterized. (usda.gov)
In this current paper, we have cloned and sequenced the full length cDNAs for ER? (usda.gov)
In the present study, six cDNAs encoding amphibian skin antimicrobial peptide precursors from the Chinese brown frog Rana chensinensis , were cloned and identified as preprobrevinin-1CEa, preprobrevinin-1CEb, preprotemporin-1CEa, preprotemporin-1CEb, preprotemporin-1CEc, and preprochensinin-1. (bioone.org)

Nuclear genome sequence and gene expression of an intracellular fungal endophyte stimulating the growth of cranberry plants. (umontreal.ca)
The genome sequence of the multinucleocapsid nucleo-polyhedrovirus of the Chinese oak silkworm Antheraea pernyi. (scirp.org)
A Bacillus anthracis Genome Sequence from the Sverdlovsk 1979 Autopsy Specimens. (cdc.gov)

We report the molecular cloning of DNA complementary to human Hp mRNA. (rti.org)
report the molecular cloning of Painting of fourth (Pof), which encodes a protein that specifically paints chromosome 4 (Proc Natl Acad Sci USA 2001, 98:6273-6278). (the-scientist.com)

In summary, the overarching rationale of SATURN was to improve methodological standards and conduct research that will help to better understand the impact of antibiotic use on acquisition, selection and transmission of AMR in different environments, by combining analyses of molecular, individual patient-level and ecologic data. (europa.eu)
Also, SATURN had as mission to improve methodological standards and conduct research to better understand the impact of antibiotic use on acquisition, selection and transmission of antibiotic-resistant bacteria (ARB) in different environments, by combining state-of-the-art analyses of molecular, ecologic and individual patient-level data. (europa.eu)
Currently, with the application of molecular biology resources in human identification, it became possible to identify a person even without physical ante-mortem data or with deteriorated biological material in negligible amounts, which are actually relatively common conditions in forensic analyses 7,12,16,19 . (bvsalud.org)
Bioinformatic analyses of DNA- and protein sequences. (lu.se)

When it was fused with the N-terminally His-tagged protein, it gave rise to accumulation of an expected 22-kDa polypeptide, in good agreement with the predicted molecular weight. (geneticsmr.com)
During further processing, the 110-kd polypeptide is reduced to a molecular weight of 84 kd. (medscape.com)

To generate a more representative panel of genetically diverse envelope genes, we PCR amplified, cloned, and sequenced complete gp160 coding regions of 35 primary (peripheral blood mononuclear cell-propagated) HIV-1 isolates collected at major epicenters of the current AIDS pandemic. (duke.edu)
The Core serves as a centralized resource for cloning and sequencing of genes. (unc.edu)
In addition, long-read sequencing of one representative XDR ST235 isolate identified an integron carrying multiple resistance genes (including bla VIM-2), with differences in gene composition and synteny from the P. aeruginosa class 1 integrons described previously. (who.int)

The minor repetitive sequences are present in the genomes of the other seven Apodemus species. (edu.au)
The inotocin/receptor couple can also be found in the recently sequenced genome from the parasitic wasp Nasonia vitripennis but not in any other holometabolous insect with a completely sequenced genome (12 Drosophila species, the malaria mosquito Anopheles gambiae, the yellow fever mosquito Aedes aegypti, the silk worm Bombyx mori, and the honey bee Apis mellifera), suggesting that this neuropeptide system is confined to basal holometabolous insects. (lu.se)
Although malaria in Yemen is caused mainly by Plasmodium falciparum and Plasmodium vivax , there are no sequence data available on the two species. (biomedcentral.com)
This study was conducted to investigate the distribution of the Plasmodium species based on the molecular detection and to study the molecular phylogeny of these parasites. (biomedcentral.com)
Much work on molecular phylogeny has focused on the relationship between Plasmodium species and the origin of human malaria [ 5 - 7 ]. (biomedcentral.com)

A second gene-specific primer was designed and used in 5ʹ RACE PCR to clone the 5՛ region. (microbiologyresearch.org)

This analysis identified 20 clones, including 1 from each subtype (or recombinant), which expressed fully functional envelope glycoproteins. (duke.edu)
Molecular cloning and Western blot analysis of mouse podocin. (jci.org)
In: Expressed sequence tags - generation and analysis, Methods in Molecular Biology Vol. 533 (J. Parkinson, ed. (umontreal.ca)
W. B. Wang, S. Y. Zhu, L. Q. Wang, F. Yu, Shen W. D. (2005) Cloning and sequence analysis of the Antheraea pernyi nucleo-polyhedrovirus gp64 gene. (scirp.org)
S. Gomi, K. Majima, Maeda S. (1999) Sequence analysis of the genome of Bombyx mori nucleopolyhedrovirus. (scirp.org)
NEB continues to expand its product offerings into areas related to PCR, gene expression, sample preparation for next generation sequencing, synthetic biology, glycobiology, epigenetics and RNA analysis. (prnewswire.com)
Sequence alignment analysis showed 5-14.8% SNP in the partial sequences of the 18 s rRNA of P. falciparum . (biomedcentral.com)
After molecular analysis through PCR technique, it was verified that DNA amplification of the samples was not possible at any of the periods and temperatures analyzed. (bvsalud.org)
Methods for gene identification and analysis of gene structure: cloning, PCR, restriction mapping, in situ hybridisation, DNA sequencing. (lu.se)

As a Senior Molecular Biologist, you will be responsible for developing and implementing molecular biology techniques to support our research programs. (jobs.ac.uk)
Training in molecular biology techniques. (unc.edu)

Developing a framework to assess the costeffectiveness of COMPARE - a global platform for the exchange of sequence-based pathogen data. (eurosurveillance.org)

Experience with Illumina sequencing platforms and library preparation methods. (biospace.com)
My current research focus is biomarker discovery of graft rejection in solid organ transplant patients and studying natural killer cells in viral infection using omics methods including Mass Cytometry and Next Generation Sequencing. (stanford.edu)
We report the methods used for marker development, along with the primer sequences and optimized PCR and thermal cycling conditions. (mdpi.com)
In this review, the molecular methods and toolkits developed for S. cerevisiae are discussed in terms of their contributions to the required standardization efforts. (bvsalud.org)

The POF protein sequence contains a putative RNA-binding domain. (the-scientist.com)

The sequence data of mouse podocin are available from GenBank/EMBL/DDBJ under accession no. (jci.org)
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. (origene.com)

Contribute to the development of novel antibody sequencing approaches and workflows for both bulk and single cells. (biospace.com)
Sequencing these antibodies provides a high-resolution view of how the human immune system responds to an influenza vaccine, how each specific antibody functions, and what part of the influenza virus they target. (cdc.gov)

The Molecular Biology Core was established to provide expertise, equipment, training, and reagents to serve the molecular biology needs for the Marsico Lung Institute/UNC Cystic Fibrosis Center. (unc.edu)
NEB has supplied DNA assembly reagents for over 40 years and the new NEBuilder HiFi DNA Assembly Master Mix is the result of our commitment to producing high quality, innovative cloning reagents. (prnewswire.com)

The SATURN project had as aim to study the impact of antibiotic exposure on AMR with a multidisciplinary approach that bridges molecular, epidemiological, clinical and pharmacological research. (europa.eu)

They have complementary skills in the fields of molecular and cellular virology, immunology, and physiopathology of infectious diseases. (europa.eu)

The Homingbac bacu-lovirus cloning system: An alternative way to introduce foreign DNA into baculovirus genomes. (scirp.org)

In your first year, your modules give you an insight into various biological and chemical disciplines, including biochemistry, cell and molecular biology, microbiology and physiology. (kent.ac.uk)

The gcsap open reading frame encodes a protein with a 78 residue prepro-sequence typical of other fungal secreted aspartic proteinases. (microbiologyresearch.org)

The type II minor repetitive sequence also hybridized interspersedly in the non-centromeric regions. (edu.au)
repetitive sequence elements in the members had been in contact with bacterial genome have shown con- patients for several hours. (who.int)

IPSWICH, Mass. , Jan. 30, 2015 /PRNewswire/ -- New England Biolabs (NEB®) announces the introduction of the NEBuilder HiFi DNA Assembly Cloning Kit and Master Mix for one-step cloning and multiple DNA fragment assembly in as little as 15 minutes. (prnewswire.com)
The same reagent allows fast, virtually error-free standard cloning, as well as large, complex multi-fragment DNA assemblies. (prnewswire.com)

For reference most cloning programs will import a .gb (Genbank) file and will show all of the plasmids features automatically when downloaded and imported. (sigmaaldrich.com)
Plasmids are an important tool in molecular biology. (tmcnet.com)
High quality plasmids supported by fully-automated platform, 100% full insert sequence accuracy guaranteed. (genscript.com)

Molecular data have been an integral tool in the resolution of the evolutionary relationships and systematics of freshwater mussels, despite the limited number of nuclear markers available for Sanger sequencing. (mdpi.com)

The N-terminal sequence, 23 residues long, was used to design a gene-specific primer. (microbiologyresearch.org)

In: RNA Metabolism in Mitochondria, book series "Nucleic Acids and Molecular Biology" (eds J.Cruz-Reyes, MW. (umontreal.ca)

This will be coupled with next generation sequencing based approaches to understanding the consequences of perturbing immune cell function. (lu.se)

The Imprint Ultra Chromatin IP Kit is Sigma′s second generation chromatin immunoprecipitation (ChIP) kit developed for maximum sensitivity and optimum next-generation sequencing results. (sigmaaldrich.com)
The locus at chromosome 1q21 was identified by linkage mapping in 1998, but the gene has only recently been discovered due to difficulty with sequencing this highly repetitive region and was previously missed using next-generation sequencing. (medscape.com)

CDC has developed a process that allows scientists to sequence the antibodies from the human B cells that make up part of the immune response to influenza viruses. (cdc.gov)

In this work, we used ochratoxin A (OTA) as a model system to study the capacity of PD-IPCR in the detection of toxic small molecular weight compounds, especially mycotoxins. (cdc.gov)
This study provides a new idea for the ultrasensitive detection of mycotoxins and other toxic small molecular weight compounds. (cdc.gov)

Having trained in the disciplines of immunology, molecular biology, and biotechnology, I have always been interested in applying state-of-the-art technologies to study human diseases and conditions. (stanford.edu)

The technical platform includes a general molecular laboratory and a class2 cellular laboratory at A11 BMC Lund. (lu.se)

It's the most powerful molecular motor known. (lu.se)

The sequence shares 90% identity with the human APJ receptor and 31% with the rat AT(1A) angiotensin receptor. (nih.gov)
Here, we report that the recently sequenced genome from the red flour beetle Tribolium castaneum contains a gene coding for an oxytocin/vasopressin-like peptide, identical to the locust peptide, which we named inotocin (for insect oxytocin/vasopressin-like peptide) and a gene coding for an inotocin G protein-coupled receptor (GPCR). (lu.se)
Furthermore, we identified an oxytocin/vasopressin-like peptide and receptor in the recently sequenced genome from the water flea Daphnia pulex (Crustacea). (lu.se)
To our knowledge, this is the first report on the molecular cloning of an oxytocin/vasopressin-like receptor and its ligand from arthropods. (lu.se)

abstract = "Three novel families of repetitive DNA sequences were molecularly cloned from the Korean field mouse (Apodemus peninsulae) and characterized by chromosome in-situ hybridization and filter hybridization. (edu.au)

At the core of OmniAb is the ability to engineer animals to generate affinity matured human sequence antibodies for use in conventional and novel therapeutic formats. (biospace.com)

Biosciences' research excellence will be further enhanced by a new 'Institute of Biotechnology and Molecular Medicine' research facility. (kent.ac.uk)

has been identified as the 2nd most consensus (ERIC) sequences which · 60 environmental samples were tak- frequent organism causing ventilator- are common to Gram-negative enteric en throughout the ICU, including associated pneumonia, the 4th most bacteria [11,12]. (who.int)

Anatomy15

Organisms18

Chemicals and Drugs38

Analytical, Diagnostic and Therapeutic Techniques and Equipment23

Phenomena and Processes66

Technology, Industry, Agriculture1

Information Science4

Health Care1

CDNA5

Characterization2

Whole-genome5

Genetics1

Genomic4

Amino12

Nucleotide sequence2

Bioinformatics1

Polymerase Chain Re1

Epidemiology3

Homology2

Genetic6

Gene sequences1

Ribosomal2

Phylogenetic2

Mechanisms4

Strains1

Fragments3

Proteins1

CDNAs3

Genome sequence3

Report the molecular2

Analyses4

Polypeptide2

Genes3

Species5

RACE-PCR1

Analysis9

Biology techniques2

Pathogen1

Methods4

Protein sequence1

Accession2

Antibody2

Reagents2

Pharmacological1

Virology1

Genomes1

Cell and mol1

Encodes a protein1

Repetitive sequence2

Fragment2

Plasmids3

Data1

Primer1

Metabolism1

Approaches1

Next-generation2

Influenza1

Detection2

Immunology1

Laboratory1

Powerful molecular1

Receptor4

Abstract1

Human1

Biotechnology1

Organism1