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CapillariesElectrophoresis, CapillaryElectrophoresis, Polyacrylamide GelElectrophoresisElectrophoresis, Gel, Two-DimensionalCapillary ActionElectrophoresis, Gel, Pulsed-FieldElectrophoresis, Agar GelElectrophoresis, MicrochipMolecular WeightCapillary PermeabilityHemangioma, CapillaryCapillary ResistanceCapillary ElectrochromatographyElectrophoresis, DiscElectrophoresis, Cellulose AcetateBlood Protein ElectrophoresisElectrophoresis, Starch GelMolecular Sequence DataIsoelectric FocusingCapillary Leak SyndromeChromatography, High Pressure LiquidChromatography, GelAnxiety, SeparationChromatography, Ion ExchangeAmino AcidsDenaturing Gradient Gel ElectrophoresisKineticsCattleCell SeparationImmunomagnetic SeparationAmino Acid SequenceHydrogen-Ion ConcentrationChromatography, AffinityDNA, BacterialMicroscopy, ElectronBase SequenceMass SpectrometryIsoelectric PointCapillary FragilityChromatographyMicrocirculationSodium Dodecyl SulfatePolymerase Chain ReactionRetinal VesselsReproducibility of ResultsProteomicsRabbitsTime FactorsBacterial ProteinsChromatography, LiquidCentrifugation, Density GradientBacterial Typing TechniquesProteomeProteinsSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationMacromolecular SubstancesElectrophoresis, PaperDNATemperatureEscherichia coliImmunoelectrophoresisMaternal DeprivationImmunodiffusionSensitivity and SpecificityLimit of DetectionChromatography, GasMethodsDNA FingerprintingNeovascularization, PhysiologicSpecies SpecificityChemical FractionationChromatography, DEAE-CelluloseBuffersCapillary TubingPeptidesTrypsinSpectrometry, Mass, Electrospray IonizationSubstrate SpecificityEndothelium, VascularGlycoproteinsCells, CulturedSequence Analysis, DNAChemistryBlood ProteinsCarbohydratesPeptide FragmentsIndicators and ReagentsSpectrophotometry, UltravioletChemical PhenomenaKidney GlomerulusMutationErythrocytesCell LineCell MembraneCorrosion CastingLiverNailsDensitometryUltracentrifugation
Mass spectrometryElectrochromatographyHPLCAffinity capillary electrophoresisAnalytesCharacterizationChiral separationMicrofluidicIonicDetectionEnantiomericISSNSelectivityEnantiomersAdsorptionCyclodextrinsReagentsIsoelectricBufferChromatographicMethodsTemperatureCompoundsLiquidZoneMicrofluidicsAnalysisTechniquesSensitivityClinicalAssayAmpliconsFocusingSpecificityVoltageElectrodeAnalysesSystemProteinsMethodTechniqueDetectFieldApplicationIndependentlySegmentSpecific
Mass spectrometry9
  • Capillary electrophoresis-mass spectrometry (CE-MS) is an analytical chemistry technique formed by the combination of the liquid separation process of capillary electrophoresis with mass spectrometry. (wikipedia.org)
  • Understanding of CE, the interface setup, ionization technique and mass detection system is important to tackle problems while coupling capillary electrophoresis to mass spectrometry. (wikipedia.org)
  • The original interface between capillary zone electrophoresis and mass spectrometry was developed in 1987 by Richard D. Smith and coworkers at Pacific Northwest National Laboratory, and who also later were involved in development of interfaces with other CE variants, including capillary isotachophoresis and capillary isoelectric focusing. (wikipedia.org)
  • In this webinar, the benefits of integrating cutting-edge microfluidic technology with capillary electrophoresis (CE) and electrospray ionization (ESI) for enhanced mass spectrometry (MS) analysis is presented. (thermofisher.com)
  • Analytical study of different species by separation techniques coupled to optical detectors and mass spectrometry in order to perform quantitative determinations of these species in different real matrices, as well as the determination of their metabolites, enantiomers and speciation. (uclm.es)
  • Capillary electrophoresis connected to mass spectrometry proved to be innovative, as it had never before been used for the analysis of the phenolic fraction of honey and walnut. (news-medical.net)
  • The ability to couple mass spectrometry to other separation techniques such as capillary electrophoresis, GC, and HPLC makes it a versatile analytical tool for the simultaneous separation and identification of analytes. (sigmaaldrich.com)
  • The aim of the course is to enable students to acquire in depth theoretical and practical knowledge of common modern separation techniques, such as chromatography, mass spectrometry and capillary electrophoresis. (lu.se)
  • These presumptive positive samples can be analyzed by second-tier screening assays employing separations such as liquid chromatography-mass spectrometry (LC-MS/MS), which increases test specificity and drastically reduces false positive referrals. (cdc.gov)
Electrochromatography11
  • The thesis focuses on the development of new homogeneous gels for the separation of drug enantiomers, peptides, DNA and virus by electrophoresis and electrochromatography in capillaries and microchips. (dissertations.se)
  • i) the charge/size separation of cationic particles as cationic species by CE, (ii) an easy fabrication method of stationary phases in micrometer-sized capillaries, which enables high reproducible chiral separations in packed capillary electrochromatography, and (iii) high selective separation and high sensitive detection of proteins with multi-functional particles. (go.jp)
  • Capillary Electrochromatography" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (umassmed.edu)
  • This graph shows the total number of publications written about "Capillary Electrochromatography" by people in this website by year, and whether "Capillary Electrochromatography" was a major or minor topic of these publications. (umassmed.edu)
  • Below are the most recent publications written about "Capillary Electrochromatography" by people in Profiles. (umassmed.edu)
  • Researchers have synthesized a chiral metal-organic framework (MOF) called L-histidine-zeolitic imidazolate framework-67 (L-His-ZIF-67) for enantioseparation of drugs in capillary electrochromatography. (chromatographyonline.com)
  • This novel MOF was used as a chiral stationary phase in open-tubular capillary electrochromatography to achieve the enantioseparation of drugs, offering promising possibilities for pharmaceutical analysis. (chromatographyonline.com)
  • Electrochromatography is a hybrid separation technique that combines the principles of both chromatography and electrophoresis. (chromatographyonline.com)
  • Yingxiang Du, the lead author of the study, highlighted the significance of their research, stating that the work showcases the successful synthesis and application of a chiral metal-organic framework for enantioseparation in capillary electrochromatography. (chromatographyonline.com)
  • The utilization of the chiral MOF, L-His-ZIF-67, as a chiral stationary phase provides a novel avenue for achieving high-resolution enantioseparation in open-tubular capillary electrochromatography. (chromatographyonline.com)
  • Du, Y. A chiral metal-organic framework synthesized by the mixture of chiral and non-chiral organic ligands for enantioseparation of drugs by open-tubular capillary electrochromatography. (chromatographyonline.com)
HPLC1
  • The laboratory analyses were performed using the separation techniques of capillary electrophoresis and HPLC coupled to different types of detectors (UV-Vis, MS, NMR). (news-medical.net)
Affinity capillary electrophoresis2
  • Some of our current work involves the development of bead-based assays, chromatography on chips, novel materials for microfluidics, new valve actuators, affinity capillary electrophoresis (ACE) and surface plasmon resonance (SPR)-CE on chips. (calstatela.edu)
  • In order to better understand the interaction mechanism between the selected CD and the analytes, the classical affinity capillary electrophoresis method, although not fully satisfactory because of ionic strength variations within a series of mobility shift measurements, was applied to estimate complexation constants and complex mobilities. (unboundmedicine.com)
Analytes4
  • For loading the analytes, the capillary is firstly placed into sample vial. (wikipedia.org)
  • Analytes migrate from one end of capillary to other based on their charge, viscosity and size. (wikipedia.org)
  • The separation and detection of analytes can be improved with better interface. (wikipedia.org)
  • It involves the separation of analytes based on their differential partitioning between a stationary phase and a mobile phase under the influence of an electric field. (chromatographyonline.com)
Characterization2
  • The portable size ZipChip Interface directly mounts onto select models of Thermo Scientific mass spectrometers, and creates a seamless CE/ESI-MS workflow that offers fast CE separation, nano-spray level sensitivity, and HRAM spectrometry for the characterization of intact proteins, antibody drug conjugates (ADCs), antibody subunits, peptides, and metabolites. (thermofisher.com)
  • For establishment of separation methods with lipid nanotechnology, characterization methods for separation field, lipid self-assembled structures were described. (go.jp)
Chiral separation1
  • The successful synthesis and application of L-His-ZIF-67 in enantioseparation further expands the possibilities for chiral separation techniques, contributing to the advancement of pharmaceutical analysis. (chromatographyonline.com)
Microfluidic3
  • Integrate capillary electrophoresis (CE) and electrospray ionization (ESI) into a single microfluidic device to rapidly prepare, separate, and electrospray biological samples directly into your Thermo Scientific mass spectrometer . (thermofisher.com)
  • In microfluidics we are focused on developing new microfluidic devices (MDs) for use in chemical and biochemical separations. (calstatela.edu)
  • Analyses were carried out in positive mode using a commercially available microfluidic capillary electrophoresis (CE) system coupled to a high-resolution mass spectrometer (HRMS). (cdc.gov)
Ionic1
  • We introduce two new dedicated certified reference materials (CRMs) and their analysis by GC using an ionic liquid capillary column, providing unique selectivity & stability. (sigmaaldrich.com)
Detection3
  • The metabolomics instruments segment is further categorized into separation tools and detection tools. (marketsandmarkets.com)
  • Capillary zone electrophoresis was employed for the determination of pyruvate in human sweat using electrochemical detection with a carbon fiber microdisk bundle electrode at a constant potential of 1.60 V vs. saturated calomel electrode. (unboundmedicine.com)
  • Capillary gel electrophoresis generally allows higher resolution, higher detection sensitivity and easier integration with other separation and detection techniques. (bvsalud.org)
Enantiomeric1
ISSN1
  • A special issue of Separations (ISSN 2297-8739). (mdpi.com)
Selectivity1
  • Jimidar, M, Fernandes De Aguiar, P, Pintelon, S & Massart, D 1997, ' Selectivity optimization for the separation of chlorophenols in an irregularly shaped experimental region in capillary electrophoresis ', Unknown Journal . (vub.be)
Enantiomers3
  • Enantiomers of amino acids and peptides were derivatized with a fluorescent chiral reagent 4-(3-isothiocyanatopyrrolidin-1-yl)-7-nitro- 2,1,3-benzoxadiazole [R-(-)- or S-(+)-NBD-PyNCS] and the resulting diastereomeric derivatives separated by capillary electrophoresis (CE). (illinois.edu)
  • Separation of etodolac enantiomers by capillary electrophoresis. (uah.es)
  • Separation of etodolac enantiomers, which exhibit different biological activity and pharmacokinetic profiles, has been achieved using the randomly substituted (2-hydroxy)propyl-β-cyclodextrin (HP-β-CD) as chiral selector in capillary electrophoresis. (uah.es)
Adsorption1
  • Lectures and exercises (7.5 credits): In-depth theoretical treatment of chromatographic separation and underlying distribution and adsorption equilibria. (lu.se)
Cyclodextrins1
  • Specifically, cyclodextrins, which are popular chiral selectors used in capillary electrophoresis, are added to the TGF buffer and cause the two stereoisomers of a chiral molecule to focus at different points in the capillary. (nist.gov)
Reagents2
  • Sebia, the global leader in clinical electrophoresis equipment and reagents, offers both capillary separation and agarose gel technologies. (jspargo.com)
  • The advantages of CE lie mostly in the ease of sample preparation, low consumption of reagents, high separation performance, and very short periods of analysis. (cdc.gov)
Isoelectric1
Buffer4
  • The type and the concentration of the chiral selector added to the BGE, the buffer pH, the type of organic modifier and its concentration, the capillary temperature and the applied voltage played a very important role in the enantioresolution of the analysed compounds. (unich.it)
  • The effect on the enantioresolution of the buffer concentration and of the degree of substitution (DS) and concentration of the CD as well as of instrumental parameters, such as the capillary temperature and the separation voltage, were studied. (uah.es)
  • The optimum separation conditions are 3.6 x 10(-3) mol/L Na2HPO4-1.4 x 10(-3) mol/L NaH2PO4 (pH 7.2) for the buffer solution, and 18 kV for the separation voltage. (unboundmedicine.com)
  • The researchers optimized various separation conditions such as pH value, buffer concentration, and the proportion of organic modifier. (chromatographyonline.com)
Chromatographic1
  • The major disadvantage of the agarose support is its poor mechanical strength which excludes its use in high speed separations where small particles are needed in order to achieve improved chromatographic efficiency. (dissertations.se)
Methods5
Temperature1
  • Temperature gradient focusing (TGF) involves application of a temperature gradient across a microchannel or capillary. (nist.gov)
Compounds4
  • All the studied compounds were enantioresolved employing a capillary column packed with vancomycin stationary phase by nano-LC, and the resolution was strongly influenced by the concentration of the organic modifier and by the pH of the mobile phase. (unich.it)
  • They have used two new techniques, capillary electrophoresis and high resolution liquid chromatography, that have enabled them to identify and quantify a great part of the phenolic compounds contained in these foods. (news-medical.net)
  • A large number of these biocide compounds can be successfully analyzed using capillary electrophoresis (CE) under the proper conditions. (cdc.gov)
  • GC/MS analysis allows separation of compounds based on their retention time within a chromatography column and identification of each compound by the characteristic fragments into which a given chemical is broken following ionization of the compound. (medscape.com)
Liquid2
Zone3
  • Two original products, Eprex (epoetin alfa) and Dynepo (epoetin delta), and two biosimilar products, Binocrit (epoetin alfa) and Retacrit (epoetin zeta), were compared using (1) high performance size exclusion chromatography, (2) ELISA, (3) SDS-PAGE, (4) capillary zone electrophoresis and (5) in-vivo potency. (springer.com)
  • Because a limited amount of EPO products was available, we were restricted to the common assays used for quality control: high performance size exclusion chromatography (HP-SEC) to assess monomer and aggregate content, ELISA to determine EPO protein content, SDS-PAGE under non-reducing conditions to detect possible fragmentation and covalent protein aggregates, capillary zone electrophoresis (CZE) for isoform profiling and a normocythemic mouse assay to test for potency. (springer.com)
  • This type of separation media offers high resolution and small zone broadening. (dissertations.se)
Microfluidics1
Analysis6
  • CE-MS combines advantages of both CE and MS to provide high separation efficiency and molecular mass information in a single analysis. (wikipedia.org)
  • In this case several samples are loaded hydrodynamically to the separation capillary before the analysis and each sample segment is placed between background electrolyte spacers. (wikipedia.org)
  • Compared to untreated and commercial concentrationplates, the surface treated plates gave on average twice the signal to noisefor peptide analysis.Signal suppression and signal overlap in MS can obscure analyte signals.In Paper II, an automated system for coupling capillary electrophoresis toMALDI-MS was developed. (kth.se)
  • Additionally, because of the very small diameters of the capillaries used for separation, CE is often not suited for trace analysis. (cdc.gov)
  • The chiral MOF demonstrated excellent separation efficiency for five chiral drugs, offering promising possibilities for pharmaceutical analysis and quality control. (chromatographyonline.com)
  • The absence of separation prior to MS/MS analysis can lead to false positives and inconclusive results due to interferences by nominal isobars and isomers. (cdc.gov)
Techniques2
  • This research has shown the potential of these techniques for the separation, identification and quantification of the phenolic fraction of vegetable matrices, using appropriate methodologies for this purpose and in the case of olive oil, studying certain technical parameters that affect the phenolic profile. (news-medical.net)
  • Gas chromatography with capillary columns and different injection techniques. (lu.se)
Sensitivity1
  • Despite of its superior performances, CE still has major problems to be overcome such as non-specific interactions between capillary surfaces and samples, difficulty in fabricating stationary phases inside micrometer-sized capillaries, and its lower sensitivity. (go.jp)
Clinical1
  • Interpretation of hemoglobin electrophoresis results should be placed in the clinical context, including the family history and results of serum iron studies, red cell morphology, hemoglobin, hematocrit, and red cell indices (eg, mean corpuscular volume). (medscape.com)
Assay1
  • Therefore, many presumptive positive specimens require confirmation by a higher specificity second-tier assay employing separations, which require additional time and resources prior to patient follow-up. (cdc.gov)
Amplicons1
Focusing1
  • In this work, the capabilities of TGF have been extended to include focusing and separation of chiral molecules. (nist.gov)
Specificity1
  • The direct spectroscopy of the original food matrix without separation was not possible in the past, because the resulting spectra are difficult to interpret and often lack specificity. (pdf-archive.com)
Voltage1
  • In this method a high voltage is applied to the sample solution and molecules are loaded to the CE capillary by electromigration and electroosmotic flow of the sample. (wikipedia.org)
Electrode1
  • In the first CE-MS interface a stainless steel capillary sheath around the separation capillary terminus was used instead of terminus electrode in typical CE setup. (wikipedia.org)
Analyses1
  • Chromatography is a powerful technique, essential in chemical analyses and preparative separation in industry and research. (dissertations.se)
System1
  • Under the optimized conditions, the enantioseparation system demonstrated excellent separation efficiency, yielding high resolutions for five chiral drugs: esmolol (7.93), nefopam (3.03), salbutamol (2.42), scopolamine (1.08), and sotalol (0.81). (chromatographyonline.com)
Proteins1
  • Then, some our results of separation with lipid nanotechnology were introduced, especially emergence of chiral recognition in lipid bilayer by polymerization, the application of lipid self-assembled structures for flow separation systems, and membrane proteins separation in lipid nano-membrane. (go.jp)
Method2
  • Furthermore, the course aims to enable students to develop their ability to independently select and optimise a suitable separation technique/method and to present a coherent holistic view of the subject. (lu.se)
  • Alkaline and/or citrate agar electrophoresis is the commonly used method. (medscape.com)
Technique4
  • Since its introduction in 1987, new developments and applications have made CE-MS a powerful separation and identification technique. (wikipedia.org)
  • Capillary electrophoresis is a separation technique which uses high electric field to produce electroosmotic flow for separation of ions. (wikipedia.org)
  • The latter was achieved with partial filling technique using ultrashort separation zones (a few mm). (unboundmedicine.com)
  • Lipid nanotechnology" is a technique utilizing superior properties of lipid self-assembled structures for separation. (go.jp)
Detect1
Field3
  • To date, the development of a chiral L-His-ZIF-67 coated capillary column for capillary electrophoresis had not been reported in the field. (chromatographyonline.com)
  • Overview of capillary electrophoresis and field flow fractionation. (lu.se)
  • Separation of hemoglobins is based on variable rates of migration of charged hemoglobin molecules in an electrical field. (medscape.com)
Application1
  • The widespread use of separation tools in research activities, increase in funds for research projects, development of innovative technologies in these tools, and their extensive application in the drug discovery process are fueling the growth of this segment. (marketsandmarkets.com)
Independently1
  • Do literature search then perform electrophoresis and kinetics experiments independently or as a team in a safe manner and report and results using scientific writing. (uaeu.ac.ae)
Segment1
  • The separation tools segment accounted for the largest share of the metabolomics market in 2019. (marketsandmarkets.com)
Specific2
  • This review focuses on researches regarding separation sciences utilizing the specific properties of microscopic separation fields. (go.jp)
  • Through these experiments, they were able to preliminarily speculate on the specific interaction forces involved in the separation process. (chromatographyonline.com)