• Two original products, Eprex (epoetin alfa) and Dynepo (epoetin delta), and two biosimilar products, Binocrit (epoetin alfa) and Retacrit (epoetin zeta), were compared using (1) high performance size exclusion chromatography, (2) ELISA, (3) SDS-PAGE, (4) capillary zone electrophoresis and (5) in-vivo potency. (springer.com)
  • Because a limited amount of EPO products was available, we were restricted to the common assays used for quality control: high performance size exclusion chromatography (HP-SEC) to assess monomer and aggregate content, ELISA to determine EPO protein content, SDS-PAGE under non-reducing conditions to detect possible fragmentation and covalent protein aggregates, capillary zone electrophoresis (CZE) for isoform profiling and a normocythemic mouse assay to test for potency. (springer.com)
  • Capillary electrochromatography is a combination of two analytical techniques, high-performance liquid chromatography and capillary electrophoresis. (wikipedia.org)
  • Achieve better gas chromatography separations with an appropriate column. (sigmaaldrich.com)
  • Some of our current work involves the development of bead-based assays, chromatography on chips, novel materials for microfluidics, new valve actuators, affinity capillary electrophoresis (ACE) and surface plasmon resonance (SPR)-CE on chips. (calstatela.edu)
  • The high separation power and short analysis time obtained demonstrate for the first time that supercritical fluid chromatography is a fast and reliable technique for the analysis of lignin-derived phenols in complex environmental samples. (lu.se)
  • The aim of the course is to enable students to acquire in depth theoretical and practical knowledge of common modern separation techniques, such as chromatography, mass spectrometry and capillary electrophoresis. (lu.se)
  • Gas chromatography with capillary columns and different injection techniques. (lu.se)
  • These presumptive positive samples can be analyzed by second-tier screening assays employing separations such as liquid chromatography-mass spectrometry (LC-MS/MS), which increases test specificity and drastically reduces false positive referrals. (cdc.gov)
  • Separation is achieved by electrophoretic migration of solutes and differential partitioning. (wikipedia.org)
  • Separation of components in CEC is based on interactions between the stationary phase and differential electrophoretic migration of solutes. (wikipedia.org)
  • The analytes separate as they migrate due to their electrophoretic mobility, and are detected near the outlet end of the capillary. (wikipedia.org)
  • Yan, Xiaoyan, "Capillary electrophoretic separations of enzyme inhibitors with activity-based detection" (2009). (lsu.edu)
  • A capillary electrophoretic method for the separation of the aminoglutethimide (AGT) enantiomers using methylated-beta-cyclodextrin (M-beta-CD) as chiral selector is described. (unboundmedicine.com)
  • A technique for separating and detecting enzyme inhibitors was developed using capillary electrophoresis with an enzyme microreactor. (lsu.edu)
  • In 1983, the first special purpose fused silica capillary GC column was introduced. (sigmaaldrich.com)
  • Since then, an impressive list of special purpose fused silica capillary GC columns has followed. (sigmaaldrich.com)
  • Good chiral separation of the racemic mixture was achieved in less than 9 min with resolution factor Rs=2.1, using a fused-silica capillary and a background electrolyte (BGE) of tris-phosphate buffer solution (50 mmol L(-1), pH 3.0) containing 30 mgm L(-1) of M-beta-CD. (unboundmedicine.com)
  • In order to better understand the interaction mechanism between the selected CD and the analytes, the classical affinity capillary electrophoresis method, although not fully satisfactory because of ionic strength variations within a series of mobility shift measurements, was applied to estimate complexation constants and complex mobilities. (unboundmedicine.com)
  • The portable size ZipChip Interface directly mounts onto select models of Thermo Scientific mass spectrometers, and creates a seamless CE/ESI-MS workflow that offers fast CE separation, nano-spray level sensitivity, and HRAM spectrometry for the characterization of intact proteins, antibody drug conjugates (ADCs), antibody subunits, peptides, and metabolites. (thermofisher.com)
  • In chemical analysis, capillary electrochromatography (CEC) is a chromatographic technique in which the mobile phase is driven through the chromatographic bed by electro-osmosis. (wikipedia.org)
  • The GC column is the heart of a GC system, where chromatographic separation occurs. (sigmaaldrich.com)
  • Capillary Gas Chromatographic Separation of Urinary Organic Acids. (nist.gov)
  • This action transfers the propylated inorganic, methyl and ethyl Hg species to the head of a 30 m capillary GC column which, using He as the carrier gas, ramps the column temperature to 280°C. The order of chromatographic separation of the Hg species is based on increasing molecular weight: methylpropylmercury (derivatized methyl Hg), ethylpropylmercury (derivatized ethyl Hg), followed by dipropylmercury (derivatized inorganic Hg). (cdc.gov)
  • Lectures and exercises (7.5 credits): In-depth theoretical treatment of chromatographic separation and underlying distribution and adsorption equilibria. (lu.se)
  • We have developed a capillary electrophoresis method to characterize the QD surface ligand interactions with various surfactant systems. (edu.pl)
  • Capillary electrophoresis aims to separate analytes on the basis of their mass-to-charge ratio by passing a high voltage across ends of a capillary tube, which is filled with the analyte. (wikipedia.org)
  • The interactions between the analytes and the stationary phase and mobile phase lead to the separation of the analytes. (wikipedia.org)
  • Results indicated that both Stabilwax-DA capillary columns and Stabilwax capillary columns achieved baseline separation of analytes. (cdc.gov)
  • The type and the concentration of the chiral selector added to the BGE, the buffer pH, the type of organic modifier and its concentration, the capillary temperature and the applied voltage played a very important role in the enantioresolution of the analysed compounds. (unich.it)
  • The effect on the enantioresolution of the buffer concentration and of the degree of substitution (DS) and concentration of the CD as well as of instrumental parameters, such as the capillary temperature and the separation voltage, were studied. (uah.es)
  • The next phase of this work is devoted to the study on capillary induce phase transitions with an experimental focus on polymer solutions containing PNIPAM at the presence of hydrophobic surfaces (mesoporous silica) as a function of pH, temperature and chain length. (lu.se)
  • This work is performed using a combination of experiments and theories where a shift to the LCST (lower critical separation temperature) is presumed to occur, resulting in a capillary-induced decrease in the LCST. (lu.se)
  • Temperature gradient focusing (TGF) involves application of a temperature gradient across a microchannel or capillary. (nist.gov)
  • Several parameters affecting the separation were studied, including the type and concentration of chiral selector, buffer pH, voltage and temperature. (unboundmedicine.com)
  • In this webinar, the benefits of integrating cutting-edge microfluidic technology with capillary electrophoresis (CE) and electrospray ionization (ESI) for enhanced mass spectrometry (MS) analysis is presented. (thermofisher.com)
  • Analytical study of different species by separation techniques coupled to optical detectors and mass spectrometry in order to perform quantitative determinations of these species in different real matrices, as well as the determination of their metabolites, enantiomers and speciation. (uclm.es)
  • Separation of etodolac enantiomers, which exhibit different biological activity and pharmacokinetic profiles, has been achieved using the randomly substituted (2-hydroxy)propyl-β-cyclodextrin (HP-β-CD) as chiral selector in capillary electrophoresis. (uah.es)
  • In microfluidics we are focused on developing new microfluidic devices (MDs) for use in chemical and biochemical separations. (calstatela.edu)
  • All the studied compounds were enantioresolved employing a capillary column packed with vancomycin stationary phase by nano-LC, and the resolution was strongly influenced by the concentration of the organic modifier and by the pH of the mobile phase. (unich.it)
  • Enantiomers of amino acids and peptides were derivatized with a fluorescent chiral reagent 4-(3-isothiocyanatopyrrolidin-1-yl)-7-nitro- 2,1,3-benzoxadiazole [R-(-)- or S-(+)-NBD-PyNCS] and the resulting diastereomeric derivatives separated by capillary electrophoresis (CE). (illinois.edu)
  • Separation of etodolac enantiomers by capillary electrophoresis. (uah.es)
  • The on-column enzyme microreactor was constructed using one or two NdFeB magnets in two configurations to immobilize alkaline phosphatase-coated superparamagnetic beads with diameters of 2.8 µm inside a capillary before the detection window. (lsu.edu)
  • The method provides for complete baseline separation of the measured compounds while yielding a low limit of detection which is reproducible. (cdc.gov)
  • Capillary gel electrophoresis generally allows higher resolution, higher detection sensitivity and easier integration with other separation and detection techniques. (bvsalud.org)
  • Enzyme inhibition assays were performed by injecting a plug of inhibitor into a capillary filled with an alkaline phosphatase substrate, AttoPhos. (lsu.edu)
  • The source vial, destination vial and capillary are filled with an electrolyte such as an aqueous buffer solution. (wikipedia.org)
  • Specifically, cyclodextrins, which are popular chiral selectors used in capillary electrophoresis, are added to the TGF buffer and cause the two stereoisomers of a chiral molecule to focus at different points in the capillary. (nist.gov)
  • Electroosmosis is the motion of liquid induced by an applied potential across a porous material, capillary tube, membrane or any other fluid conduit. (wikipedia.org)
  • The relationship between the linear velocity u of the liquid in the capillary and the applied electric field is given by the Smoluchowski equation as u = ϵ r ϵ 0 ζ E η {\displaystyle u=\epsilon _{r}\epsilon _{0}\zeta E\eta } where ζ is the potential across the Stern layer (zeta potential), E is the electric field strength, and η is the viscosity of the solvent. (wikipedia.org)
  • Adhesion: liquid bridges and capillary wetting. (cornell.edu)
  • Jimidar, M, Fernandes De Aguiar, P, Pintelon, S & Massart, D 1997, ' Selectivity optimization for the separation of chlorophenols in an irregularly shaped experimental region in capillary electrophoresis ', Unknown Journal . (vub.be)
  • The capillaries/confined geometries are known to influence the phase diagram of polymer solutions where condensation of bulk solutions may occur close to the surfaces. (lu.se)
  • The following thesis provides an extensive study into the fabrication of polymer monolithic columns in capillary housing, their modification and subsequent characterisation. (dcu.ie)
  • The components of a capillary electrochromatograph are a sample vial, source and destination vials, a packed capillary, electrodes, a high voltage power supply, a detector, and a data output and handling device. (wikipedia.org)
  • Capillary columns for GC enantiomer separation. (mn-net.com)
  • Using thermally initiated co-monomer polymerisation, monolithic columns were fabricated within fused silica capillaries. (dcu.ie)
  • The nanospray system can be connected to syringe pumps in order to get a continuous nano-system equipped with capillary columns for separation on a column packed with C18 or other packing material of interest. (lu.se)
  • However, the resulting stepped gradient was not ideal for separations. (dcu.ie)
  • An alternative method for the analysis of phenol and o-, m-, and p- cresol by capillary GC/FID. (cdc.gov)
  • Furthermore, the course aims to enable students to develop their ability to independently select and optimise a suitable separation technique/method and to present a coherent holistic view of the subject. (lu.se)
  • The column was successfully applied to the separation of two metal cations. (dcu.ie)
  • Inhibitor zones electrophoresed through the capillary, passed through the enzyme microreactor, and were observed as negative peaks due to decreased product formation in the presence of the inhibitors. (lsu.edu)
  • The goal of this study was to improve peak capacities for inhibitor separations relative to previous work, which combined continuous engagement electrophoretically mediated microanalysis (EMMA) and transient engagement EMMA to study enzyme inhibition. (lsu.edu)
  • The absence of separation prior to MS/MS analysis can lead to false positives and inconclusive results due to interferences by nominal isobars and isomers. (cdc.gov)
  • Integrate capillary electrophoresis (CE) and electrospray ionization (ESI) into a single microfluidic device to rapidly prepare, separate, and electrospray biological samples directly into your Thermo Scientific mass spectrometer . (thermofisher.com)
  • The latter was achieved with partial filling technique using ultrashort separation zones (a few mm). (unboundmedicine.com)
  • Analyses were carried out in positive mode using a commercially available microfluidic capillary electrophoresis (CE) system coupled to a high-resolution mass spectrometer (HRMS). (cdc.gov)
  • Damage to cells in the basal layer of the epidermis results in separation of the epidermis from the dermis or, at high doses, in direct necrosis and sloughing of the epidermis. (msdmanuals.com)
  • A low-viscosity mobile phase consisting of compressed trial organic matter in geosciences because the composition carbon dioxide is used in SFC to achieve fast and efficient of its phenols is an important indicator of the type of original separation. (lu.se)
  • Full phase separation is prevented because RNA remains tethered to transcribed euchromatin through RNA polymerases. (nature.com)
  • Although it has recently been shown that heterochromatin segregates from euchromatin via the physical process of phase separation 15 , 16 , 17 , it remains unclear how the interspersed pattern that is characteristic of the internal organization of euchromatin is established and maintained. (nature.com)
  • A configuration where several phase domains persist and do not fuse, in these models, is described as microphase separation. (nature.com)
  • The capillary is packed with stationary phase. (wikipedia.org)
  • A large number of separations have been achieved, however, it is not possible to make a general prediction, which phase could solve a given separation task. (mn-net.com)
  • In this work, the capabilities of TGF have been extended to include focusing and separation of chiral molecules. (nist.gov)
  • The possibility to enhance resolution to infinite value in chiral capillary electrophoresis is attained as soon as the apparent mobility of one enantiomer becomes opposite to the other. (unboundmedicine.com)
  • Therefore, many presumptive positive specimens require confirmation by a higher specificity second-tier assay employing separations, which require additional time and resources prior to patient follow-up. (cdc.gov)