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CentrifugationCentrifugation, Density GradientCentrifugation, ZonalCentrifugation, IsopycnicSubcellular FractionsCell FractionationMolecular WeightMetrizamideMicroscopy, ElectronElectrophoresis, Polyacrylamide GelCell MembraneKineticsChromatography, GelUltracentrifugationCell SeparationLiverPovidoneTritiumMicrosomesSolubilityCattleHypergravityChromatographyChemical FractionationFiltrationRabbitsDetergentsHydrogen-Ion ConcentrationSucroseMethodsCell LineTime FactorsOrganoidsMolecular Sequence DataChromatography, Ion ExchangeCells, CulturedRats, Inbred StrainsAmino AcidsCytosolNucleotidasesChromatography, DEAE-CelluloseMembranesPhospholipidsChromatography, AffinityLysosomesStroke VolumeMembrane ProteinsMacromolecular SubstancesOctoxynolTemperatureElectrophoresisCarbon IsotopesAcid PhosphataseChromatography, High Pressure LiquidProteinsCesiumCell NucleusSilicon DioxideCytoplasmCell-Free SystemEscherichia coliDNAChemical PrecipitationAmino Acid SequenceProtein BindingCytoplasmic GranulesMagnesiumSpermatozoaIntracellular MembranesImmunodiffusionErythrocytesPolyethylene GlycolsCarbohydratesDiatrizoateChemistry Techniques, AnalyticalSwineDialysisRNATissue ExtractsChemistryElectrophoresis, DiscEdetic AcidFreezingChemical PhenomenaPronaseHot TemperatureMitochondriaMicrosomes, LiverBiological TransportChromatography, Thin LayerOrganellesImmune SeraRibonucleasesTriiodobenzoic AcidsCulture MediaSuccinate DehydrogenasePhosphorus IsotopesMitochondria, LiverFluorescent Antibody TechniqueColloids
Differential centrifugationDensity gradientDairy centrifugeUses centrifugalParticlesCentrifugal forceDensitiesTechniqueCellsMethodSolutionElements
Differential centrifugation1
Density gradient3
  • The tube is first filled with different concentrations of sucrose or another solute establishing layers with different densities and viscosities, forming a density gradient, within which the particles to be separated are added. (wikipedia.org)
  • A longer distance of travel may allow better separation, such as in density gradient centrifugation. (universe84a.com)
  • Normally used for density-gradient centrifugation. (universe84a.com)
Dairy centrifuge2
  • Antonin Prandtl, an 1864 inventor of the dairy centrifuge that separates cream from milk, proposed the idea. (microbiologynote.com)
  • Later in 1864, Antonin Prandtl proposed the idea of a dairy centrifuge to separate cream from milk and his brother Alexander Prandtl became able to exhibit a working butter fat extraction machine in 1875. (universe84a.com)
Uses centrifugal2
  • A centrifuge uses centrifugal force in order to separate different components from a fluid. (microbiologynote.com)
  • Biological centrifugation is a technique that uses centrifugal forces to separate and purify a mixture of biological particles in a liquid medium. (universe84a.com)
Particles6
  • Rate-zonal centrifugation is a centrifugation technique employed to effectively separate particles of different sizes. (wikipedia.org)
  • These samples would be pooled and processed by using available technology to isolate virus particles en masse, recover viral nucleic acids, produce amplified shotgun libraries, carry out shotgun sequencing of the mixture of viral genomes, and reconstruct these genomes in silico with the techniques originally developed to sequence the entire human genome from random fragments. (cdc.gov)
  • Ultracentrifuges and high-speed centrifuges that can provide high accelerations are capable of separating fine particles down to the nanoscale and molecules with different masses. (microbiologynote.com)
  • Centrifugation is a process that involves the use of a centrifuge to separate particles from a solution based on their size, shape, density, or other physical properties. (microbiologynote.com)
  • Ultracentrifuges are used to separate very small particles or to measure the size, shape, and density of particles. (microbiologynote.com)
  • A centrifuge is a piece of equipment used to separate heavier particles from lighter ones by the action of centrifugal force. (universe84a.com)
Centrifugal force1
  • The centrifuge works on the principle of gravity and the generation of the centrifugal force to sediment different fractions. (universe84a.com)
Densities2
  • The density of the P9-specific membrane fraction was lower (approximately 1.13 g/cm 3 in sucrose) than the densities of the bacterial cytoplasmic and outer membrane fractions. (biomedcentral.com)
  • It can then separate fluids with different densities (e.g. (microbiologynote.com)
Technique1
  • Centrifugation is a widely used technique in research, industry, and clinical laboratories, and it has many applications, including the purification of biological samples, the separation of cells and organelles, the analysis of proteins and nucleic acids, and the preparation of diagnostic specimens. (microbiologynote.com)
Cells4
  • The use of conventional and zonal centrifugation to study the life cycle of mammalian cells. (wikidata.org)
  • High-speed centrifuges are used to separate cells and other biological materials, while low-speed centrifuges are used to separate non-biological materials such as suspensions of crystals or inks. (microbiologynote.com)
  • The distributions of lysosomal acid hydrolases in TDL fractionated by zonal centrifugation are similar to the distribution obtained for the cells. (rupress.org)
  • It is a key method for isolating and analyzing the cells, sub-cellular fractions, supra-molecule complexes, and isolated macro-molecules such as proteins and nucleic acids. (universe84a.com)
Method1
Solution2
  • The demerit of this, the pellet may fall back into the solution at end of centrifugation. (universe84a.com)
  • This evaluate summarizes ongoing aviation medicine inspections straight into medical and also simple research research upon RBC storage space consequences.Current findingsResearch straight into feasible unhealthy scientific effects of extented storage involving RBCs has discovered the particular contribution of varied RBC production elements (electronic.h. overnight keep, centrifugation pace, storage space solution), hardly ever previously noted. (coxsignal.com)
Elements1
  • Gas centrifuges can be used to separate isotopes, such as enriching nuclear fuel for fissile elements. (microbiologynote.com)