Self renewal hematopoietic stem cells. Medical search. Frequent questions

We examined hematopoietic stem cells at four stages of differentiation, lineage-committed progenitors, and mature myeloid and lymphoid cells. (nih.gov)
The telomerase-expressing somatic cells observed in this study are not thought to be immortal, and expression was not correlated with cell cycle distribution or differentiation state. (nih.gov)
ESCs have the characteristic properties of a stem cell: self-renewal and differentiation. (wikipedia.org)
Role of insulin-like growth factors in endothelium differentiation ECs derived from stem cells are the beginning of vasculogenesis. (wikipedia.org)
It is well established that insulin-like growth factor (IGF) signaling is important for cell responses such as mitogenesis, cell growth, proliferation, angiogenesis, and differentiation. (wikipedia.org)
Differentiation is the process by which a cell becomes more specialized. (wikipedia.org)
Despite significant advances in understanding nephron segment patterning, many questions remain about the underlying genes and signaling pathways that orchestrate renal progenitor cell fate choices and regulate differentiation. (mdpi.com)
The colony-forming unit (CFU) assay is a clonal, in vitro culture assay that measures the growth and frequency of functionally viable HSPCs by assessing the proliferation and differentiation of individual progenitor cells, resulting in the formation of discrete colonies in a semi-solid methylcellulose medium (such as MethoCult™ ) when supplemented with appropriate cytokines. (stemcell.com)
Cell markers, or surface antigens, are molecules located on a cell's membrane used to identify specific cell types, their lineage, and their stage in the differentiation process according to the presence or absence of the expression of defined markers. (stemcell.com)
Aged hematopoietic stem cells (HSC) exhibit compromised reconstitution capacity and differentiation-bias towards myeloid lineage, however, the molecular mechanism behind it remains not fully understood. (haematologica.org)
Stem cells are defined by their capacity for self-renewal and multilineage differentiation, making them uniquely situated to treat a broad spectrum of human diseases. (articlecity.com)
Consequently, investigation of molecular heterogeneity in the differentiation potential of HSPC is vital to identify novel regulators that affect generation of specific cell types, especially RBC. (lu.se)
erythroid cells after in vitro differentiation, despite having similar frequencies of phenotypic HSC initially. (lu.se)
Melinda is highly proficient in the use of in vitro and in vivo assays for normal and malignant hematopoietic stem and progenitor cells, determination of self-renewal, expansion and lineage specific differentiation capabilities, and the use of induced pluripotent stem cells for the investigation of hematopoietic disorders and leukemic progression and the testing of targeted therapeutics. (edu.au)
Sequential rounds of division and differentiation give rise to an enormous amplification of cell numbers, necessary for the production of mature blood cells. (justia.com)
This process of division and differentiation is subject to regulation at many levels to control cell production. (justia.com)
My laboratory studies signal transduction pathways regulate hematopoietic stem cell (HSC) self-renewal and differentiation as well as drug resistance of hematological malignancies. (coriell.org)
A better understanding of the complex signaling network regulating the balance between HSC self-renewal and differentiation will allow us to manipulate HSCs to enhance their therapeutic efficacy. (coriell.org)
he aim of the Thomas laboratory is to understand how the balance between proliferation and differentiation of stem cells is maintained. (edu.au)
In this issue of Cancer Discovery , Dominguez and colleagues report that TET2 function is critical for germinal center exit and plasma cell differentiation, and its deficiency can lead to B-cell lymphoma phenotypes. (aacrjournals.org)
Overexpression of FLI1 in erythroblasts causes inhibition of differentiation and ultimately the development of pre-T cell lymphoblastic leukemia/lymphoma [ 11 ]. (biomedcentral.com)
Our research focuses on developmental pathways that regulate hematopoietic cell growth and differentiation and are disrupted in the course of neoplastic transformation, particularly in leukemias and lymphomas. (stanford.edu)
The modification has been shown to be critical to cell differentiation, animal development, and a range of biological signaling and stress response. (chicagobiomedicalconsortium.org)
Cellular RNA methylation level can have profound impacts on normal cell differentiation and cancer cell proliferation. (chicagobiomedicalconsortium.org)
Several genes involved in different aspects of stem cell activation are being studied, including some that transduce initial signals, and others that regulate self-renewal and differentiation. (chicagobiomedicalconsortium.org)
Steady-state hematopoietic stem cells' (HSCs) self-renewal and differentiation toward their mature progeny in the adult bone marrow is tightly regulated by cues from the microenvironment. (elsevierpure.com)
The adult bone marrow provides essential niches for hematopoietic stem cell (HSC) maintenance and differentiation, which are not fully understood. (elsevierpure.com)
Hematopoiesis, in particular the genetic control of hematopoietic cell proliferation and differentiation is also studied in human cell cultures and transgenic mouse model systems. (lu.se)
Stem and progenitor cell populations are often heterogeneous, which may reflect stem cell subsets that express subtly different properties, including different propensities for lineage selection upon differentiation, yet remain able to interconvert. (lu.se)
A key challenge is to understand how state, but must also afford flexibility in cell-fate choice to permit the different cell-fate options confronting stem and progenitor cell-type diversification and differentiation in response to cells are selected and coordinated such that adoption of a given intrinsic cues or extrinsic signals. (lu.se)
Evidence the fate of stem cells has broad ramifications for biomedical suggests that during development or differentiation, cells make science from elucidating the causes of cancer to the use of very precise transitions between apparently stable ``network stem cells in regenerative medicine. (lu.se)
The use of ASCs in regenerative medicine and tissue engineering research has important advantages in comparison with ESCs, since there are no ethical complications and the process of differentiation of these cells is better controlled. (bvsalud.org)
8,9 Because dental stem cells (DSCs) are easy to obtain and present a great potential of differentiation, there has been a growing interest in their use in regenerative medicine for treatment of various human diseases. (bvsalud.org)
Abnormal proliferation, clonal expansion, aberrant differentiation, and diminished apoptosis (programmed cell death) lead to replacement of normal blood elements with malignant cells. (msdmanuals.com)
Temporal multimodal single-cell profiling of native hematopoiesis illuminates altered differentiation trajectories with age. (lu.se)

In this study, we observed that the expression of pseudouridine (Ψ) synthase 10 is increased in aged hematopoietic stem and progenitor cells (HSPC) and enforced protein of Ψ synthase 10 (PUS10) recapitulates the phenotype of aged HSC, which is not achieved by its Ψ synthase activity. (haematologica.org)

In patients who underwent allogeneic hematopoietic stem cell transplantation (allo-HSCT), however, EFS and OS were not significantly different between FHL2 or iASPP high- and low-expression groups. (nature.com)
HSCs specifically are defined by their ability for self-renewal and to reconstitute the entire hematopoietic system following transplantation. (stemcell.com)
It is used both by hematopoietic researchers and clinical labs to assess the potency of transplantation units of cord blood (CB) and hematopoietic cellular therapy products. (stemcell.com)
Other uses of the CFU assay include studying the effects of stimulatory and inhibitory growth factors, screening novel compounds to predict potential toxicity to the hematopoietic system, and testing the effects of various in vitro manipulations (e.g. cell processing, cryopreservation, gene transduction, and transmission) on cellular products used in hematopoietic cell transplantation. (stemcell.com)
For example, because hematopoietic stem cells can reconstitute the entire blood system, bone marrow transplantation has long been used in the clinic to treat various diseases. (articlecity.com)
Similarly, the transplantation of other tissue-specific stem cells, such as stem cells isolated from epithelial and neural tissues, can treat mouse disease models and human patients in which epithelial and neural cells are damaged. (articlecity.com)
Therefore, hematopoietic stem cells mobilized into a subject's peripheral blood by the method of the invention is useful as a source of donor cells in bone marrow transplantation for the treatment of a variety of disorders, including cancer, anemia, autoimmunity and immunodeficiency. (justia.com)
Hematopoietic stem cell transplantation (HSCT) is a life-saving procedure for treatment of malignant and non-malignant disorders and is usually a last resort for those with no other treatment options available. (coriell.org)
However, sustained engraftment of the expanded cells was not observed after transplantation, suggesting ineffective expansion of hematopoietic stem cells with long-term repopulating activity (LTR-HSCs). (confex.com)
At optimal coating densities of Delta1, the frequency of LTR-HSCs measured by limiting dilution analysis 16 weeks after transplantation into NSG mice was 4.9- and 4.2-fold higher in hypoxic cultures (1 in 1,586 CD34+ cells) compared with uncultured cells (1 in 7,706) and the normoxia group (1 in 5,090), respectively. (confex.com)
Conversely, we observed no difference in expression of the homing CXCR4 receptor between cells cultured under normoxic and hypoxic conditions, indicating that hypoxia increased the absolute numbers of LTR-HSCs but not their homing potential after transplantation. (confex.com)
Her aim is to enhance understanding of these processes to generate blood stem cells in the lab, which would improve transplantation treatments for blood diseases like leukemia and aplastic anemia. (lu.se)
Treatment typically includes combination chemotherapy to achieve remission, intrathecal and systemic chemotherapy and/or corticosteroids for CNS prophylaxis, and sometimes cerebral irradiation for intracerebral leukemic infiltration, consolidation chemotherapy with or without stem cell transplantation, and maintenance chemotherapy for up to 3 years to avoid relapse. (msdmanuals.com)

Left panels show representative FACS plots of Lin - Kit hi BM cells gated for LSK and LK HSC progenitors. (jci.org)
02:14 progenitors that make red blood cells. (yale.edu)
One important aspect of this is to define the role of co-activators of transcription in stem and progenitors cells during embryonic development and in adults. (edu.au)

He leads a laboratory focused on investigating the mechanisms that underlie the development of blood cancers with a focus on studying the genetic pathways that control the proliferation, survival and self-renewal of normal or leukemic hematopoietic stem cells, and to identify novel therapeutic strategies for the treatment of myeloid malignant diseases. (lls.org)
Although HSCs are the most well-characterized tissue-specific stem cells, our understanding of the molecular pathways that control HSC maintenance and function is still limited. (coriell.org)
Cysteines are an amino acid which are involved in pathways that feed cancer cells and provide building blocks for cancer cell growth. (uclahealth.org)
In terminally differentiated cell fate is coupled to appropriate regulation of the alternative cells, transcriptional networks must be stable and irreversible, pathways. (lu.se)

These factors may regulate myeloid versus lymphoid balance with age, and can potentially mitigate the long-term deleterious effects of inflammation that lead to hematopoietic pathologies. (biorxiv.org)
The Goodell Lab focuses on the basic mechanisms that regulate hematopoietic stem (HSC) cells, and how their mis-regulation leads to leukemia development. (chicagobiomedicalconsortium.org)

We found that HSCs from Lnk-/- mice have an increased quiescent fraction, decelerated cell cycle kinetics, and enhanced resistance to repeat treatments with cytoablative 5-fluorouracil in vivo compared with WT HSCs. (jci.org)
Lnk -/- LSK HSCs show decelerated cell cycle kinetics. (jci.org)
Long-term hematopoietic stem cells (LT-HSCs) maintain hematopoietic output throughout an animal's lifespan. (biorxiv.org)
Here, we show that young and aged LT-HSCs respond differently to inflammatory stress, such that aged LT-HSCs produce a cell-intrinsic, myeloid-biased expression program. (biorxiv.org)
Using single-cell RNA-seq, we identify a myeloid-biased subset within the LT-HSC population (mLT-HSCs) that is much more common amongst aged LT-HSCs and is uniquely primed to respond to acute inflammatory challenge. (biorxiv.org)
Aged LT-HSCs demonstrate a cell-intrinsic myeloid bias during inflammatory challenge. (biorxiv.org)
HSCs make up a very small population of the hematopoietic system, however, these cells are invaluable as they have the potential to give rise to all mature blood and immune cell types and sustain life-long blood production. (stemcell.com)
CD34 is the most commonly used cell surface marker to identify human HSPCs as it is expressed on HSCs, in addition to both multipotent and more differentiated progenitor cells of individual blood cell lineages. (stemcell.com)
This puts the hematopoietic system under severe stress and pushes hematopoietic stem cells (HSCs) in the bone marrow to produce fresh cells and replenish the stable pool of differentiated blood cells in the body. (eurekalert.org)
Under normal conditions, HSCs kept dormant in the bone marrow to preserve their long-term self-renewal potential and prevent stem cell exhaustion. (eurekalert.org)
Indeed, HSCs express some receptors that could induce inflammation but they are primarily associated with immune cells and their role is to sense viral RNA. (eurekalert.org)
HSCs are able to self-renew and differentiate into all blood cell lineages. (coriell.org)
To corroborate these findings molecularly, we performed transcriptomic analyses and found significant upregulation of a distinct HSC gene expression signature in cells cultured with Delta1 in hypoxia (Fig. A). Collectively, these data show that hypoxia supports a superior ex vivo expansion of human HSCs with LTR activity compared with normoxia at optimized densities of Delta1. (confex.com)
To clarify how hypoxia improved Notch-mediated expansion of LTR-HSCs, we performed scRNA-seq of CD34+ cells treated with Delta1 under normoxic or hypoxic conditions. (confex.com)
Studies are designed to investigate whether the function and self-renewal capacity of Smurf2-deficient LT-HSC are enhanced, whether such enhanced function of HSCs provides benefit to mice during aging, and whether senescence plays a role in HSC self-renewal and regulation during aging. (umassmed.edu)
FLT3 ITD triggers the proliferation of the quiescent hematopoietic stem cell (HSC) pool but fails to directly transform HSCs. (biomedcentral.com)
Here, we review emerging evidence showing how HSCs and their progeny are regulated by an interdependent network of mesenchymal stromal cells, nerve fibers, the vasculature, and also other hematopoietic cells. (elsevierpure.com)

They are multipotent, which describes the ability to give rise to many cell types, whereas a pluripotent stem cell can give rise to all types. (wikipedia.org)
Important signaling factors for vasculogenesis are TGF-β, BMP4, and VEGF, all of which promote pluripotent stem cells to differentiate into mesoderm, endothelial progenitor cells, and then into mature endothelium. (wikipedia.org)
Oct-4 (POU5F1) is a transcription factor that is critically involved in the self-renewal of pluripotent stem cells, and its expression is commonly used as a marker for pluripotency. (stemcell.com)
It could also enable scientists to produce blood stem cells from pluripotent stem cells , which have the potential to create any cell type in the body. (medicalxpress.com)
Using pluripotent stem cells, they made blood stem cell-like cells that lacked the ability to self-renew and monitored which genes were not activated. (medicalxpress.com)
Induced pluripotent stem cells, which harness the power of genetic reprogramming - basically, the altering of a cell's DNA - to change the course of cellular development. (articlecity.com)
True stem cells are both pluripotent-that is they can give rise to all cell types-and capable of self-renewal. (justia.com)
Given the tremendous promise of regenerative medicine to enhance human health and treat disease, Congress included a provision in the 21st Century Cures Act to support a Regenerative Medicine Innovation Project ($30 million distributed over FY17 through FY20) for the funding of clinical research to further the field of regenerative medicine (RM) using adult stem cells, including autologous, non-autologous use as well as eligible induced pluripotent stem cells (iPSC). (nih.gov)
In reality, gene somatic cells to a pluripotent cell state by a handful of transcrip- expression is graded, making the potential gene expression tion factors (Takahashi and Yamanaka, 2006). (lu.se)
3 Embrionic stem cells (ESCs) are derived from the inner cell mass of the blastocyst and form all cell types, derived from the three germ layers, and are therefore pluripotent. (bvsalud.org)
Malignant transformation usually occurs at the pluripotent stem cell level, although it sometimes involves a committed stem cell with more limited capacity for self-renewal. (msdmanuals.com)

Mutations have also been found in the blood of elderly patients without hematopoietic cancers, indicating that TET2 loss alone is not sufficient to trigger leukemic transformation (reviewed in ref. 5 ). (aacrjournals.org)
Malignant transformation and uncontrolled proliferation of an abnormally differentiated, long-lived hematopoietic progenitor cell results in a high circulating number of blasts, replacement of normal marrow by malignant cells, and the potential for leukemic infiltration of the central nervous system (CNS) and testes. (msdmanuals.com)

In the February 1, 2011, issue of Genes and Development, Taoudi and colleagues (pp. 251-262) show an essential role for the ETS transcription factor ERG in the self-renewal of embryonic hematopoietic stem cells. (mdc-berlin.de)
To uncover what makes blood stem cells self-renew in a lab, the researchers analyzed the genes that turn off as human blood stem cells lose their ability to self-renew, noting which genes turned off when blood stem cells differentiate into specific blood cells such as white or red cells. (medicalxpress.com)
They then put the blood stem cells into laboratory dishes and observed which genes shut down. (medicalxpress.com)
Found within Wharton's jelly - which is easily harvested from what would otherwise be post-natal medical waste - are several distinct stem cell genes. (articlecity.com)
For example, the cell needs to switch off genes responsible for the energy-saving mode and turns on genes essential for increased metabolism or cell cycling. (eurekalert.org)
Specifically, we have been focusing on Polycomb-group (PcG) genes, which play an important role in supporting self-renewal. (karger.com)
This mechanism also applies to hematopoietic cells transformed by other HOX genes, including CDX2, which is highly expressed in a majority of acute myeloid leukemias, thus providing a molecular approach based on GSK-3 inhibitory strategies to target HOX-associated transcription in a broad spectrum of leukemias. (stanford.edu)
In addition, the generation of two GFP mutants with distinct excitation optima allows for detection of two distinct GFP genes within single cells. (umassmed.edu)
1 V(D)J recombination and the activity of genes of interest (using GFP as a reporter of gene expression) can be detected as intracellular fluorescence and are measured simultaneously in the same cell population, at the single cell level. (umassmed.edu)
explosion further, consider that a fictitious small genome with 2002) More recently and more dramatically, the potential for 260 genes would host the same number of combinations as cell state conversions is exemplified by the reprogramming of the number of atoms in the visible universe! (lu.se)
Inactivation of tumor suppressor genes, whose products normally provide negative control of cell proliferation, contributes to malignant transformation in various cell types. (medscape.com)
Like Rb protein, many of the proteins encoded by tumor suppressor genes act at specific points in the cell cycle. (medscape.com)
TP53 activates the expression of genes involved in apoptosis, cell cycle regulation (p21), and MDM2. (medscape.com)
Another important class of tumor suppressor genes involved in cell cycle control and in the generation of human cancers is the cyclin-dependent kinase (CDK) inhibitors. (medscape.com)

The role of apoptosis in the regulation of hematopoietic stem cells: Overexpression of Bcl-2 increases both their number and repopulation potential. (nature.com)
Our recent work identified a new connection between GSK3 and PPAR-δ/mitophagy pathway, which will provide new insights into the mechanisms of the regulation of HSC activity and may improve the therapy of many hematopoietic disorders. (coriell.org)
We are studying the role that normal chromatin structure plays in gene regulation in hematopoietic cells and how its disruption leads to altered development and cancer. (stanford.edu)
Using these 'senescence' mouse models, combined with different tumorigenesis models (for instance, p53 knockout, B cell lymphoma, skin cancer, colon cancer and liver cancer models), we hope to gain a better understanding of the regulation and contribution of senescence in tumor development. (umassmed.edu)
The mouse models we have generated to modulate senescence response in vivo are also used in our studies of senescence regulation in aging, with an emphasis on adult stem cells. (umassmed.edu)
The Division of Molecular Hematology (DMH) aims to generate new knowledge about the regulation of blood cell development by applying recent advances in stem cell technology, in order to dissect the molecular regulation of hematopoiesis. (lu.se)

Cell cycle gene expression networks discovered using systems biology: Significance in carcinogenesis. (genenetwork.org)
Although we've learned a lot about the biology of these cells over the years, one key challenge has remained: making human blood stem cells self-renew in the lab," she said. (medicalxpress.com)
If we think about the amount of blood stem cells needed to treat a patient, that's a significant number," said Mikkola, who is also a professor of molecular, cell and developmental biology in the UCLA College and a member of the UCLA Jonsson Comprehensive Cancer Center. (medicalxpress.com)
I am currently a Senior Scientist for Stem Cell Biology and Gene Engineering at the Coriell Institute for Medical Research. (coriell.org)
He received postdoctoral training at Stanford University School of Medicine, and joined the faculty in the Department of Cell Biology at University of Massachusetts Medical School in 2006. (umassmed.edu)
Cold Spring Harbor, NY -- Cold Spring Harbor Laboratory Press (CSHLP) announced the release of The Digital Cell: Cell Biology as a Data Science, available on its website in hardcover format. (cshlpress.com)
With degrees from the University of Helsinki, her interest in hematopoietic stem cell biology began during her post-doctoral training in Stefan Karlsson's laboratory here at Lund University (1998-2000), where she developed lentiviral gene transfer tools to manipulate stem cells. (lu.se)
Understanding cell-fate decisions in stem cell populations is a major goal of modern biology. (lu.se)

Oncogene iASPP enhances self-renewal of hematopoietic stem cells and facilitates their resistance to chemotherapy and irradiation. (nature.com)
Activation of OCT4 enhances ex vivo expansion of human cord blood hematopoietic stem and progenitor cells by regulating HOXB4 expression. (stemcell.com)
Work in knockout mouse models has shown that TET2 loss enhances hematopoietic stem cell (HSC) self-renewal, expanding the HSC pool (reviewed in refs. (aacrjournals.org)
Using a CRISPR Cas9-guided immunoprecipitation assay, we identify a circular RNA in the FLI1 promoter chromatin complex, consisting of FLI1 exons 4-2-3, referred to as FECR1.Overexpression of FECR1 enhances invasiveness of MDA-MB231 breast cancer cells. (biomedcentral.com)

To assess the role of an individual HDAC isoenzyme in physiology and tumor development, HDAC2-mutant mice were generated from a gene trap embryonic stem cell clone. (aacrjournals.org)
We discuss these properties with examples both from the hematopoietic and embryonic stem cell (ESC) systems. (lu.se)

Endothelial cells can be found throughout the whole vascular system and they also play a vital role in the movement of white blood cells ECs were first thought to arise from extraembryonic tissues because blood vessels were observed in the avian and mammalian embryos. (wikipedia.org)
The ability of the blood stem cells to regenerate was suppressed for at least one year after challenge with inflammation, suggesting that infection and inflammation may act as a prominent driver of age-associated functional decline in tissues. (eurekalert.org)
Overall, we observe that germline genetic variation shapes hematopoietic stem cell function, leading to CHIP through mechanisms that are specific to clonal hematopoiesis as well as shared mechanisms that lead to somatic mutations across tissues," co-senior authors Sekar Kathiresan and Pradeep Natarajan, both of the Broad, and their colleagues wrote in their paper. (genomeweb.com)
The subject's blood can be processed and used to repopulate the destroyed lymphohematopoietic system of a recipient and may in the future be utilized to repair a variety of non-hematopoietic tissues. (justia.com)
ASCs are present in a number of posnatal tissues and are responsible for normal tissue renewal as well as for regeneration and healing after injuries. (bvsalud.org)
Due to the ability to self-renew and to differentiate into cells that are found throughout the body, there is a great interest in using stem cells for the regeneration of injured tissues as well as to develop tissue-engineered implants and bio-hybrid organs, in order to restore tissue function. (bvsalud.org)
In human postnatal dental tissues, five main sources of DSCs have been identified: dental pulp stem cells (DPSCs), 11 stem cells from human exfoliated deciduous teeth (SHEDs), 12 periodontal ligament stem cells (PDLSCs), 13 dental follicle stem cells (DFSCs) 14 and stem cells from apical papilla (SCAPs). (bvsalud.org)

Not only is VEGF critical for mesoderm cells to become an EC, but also for EPCs to differentiate into mature endothelium. (wikipedia.org)
Stem cells can produce more stem cells (self-renewal) and also differentiate and proliferate, giving rise to red blood cells (erythrocytes), white blood cells (leukocytes), and platelets. (rarediseases.org)
Blood stem cells, also known as hematopoietic stem cells , are found in the bone marrow, where they self-renew as well as differentiate to create all types of blood cells. (medicalxpress.com)
When blood stem cells are removed from the bone marrow and placed in laboratory dishes, they quickly lose their ability to self-renew, and they either die or differentiate into other blood cell types. (medicalxpress.com)
They are able to differentiate into any cell of an organism and have the ability of self-renewal. (articlecity.com)
Stem cells (SCs) are undifferentiated cells with self-renewal ability and capacity to differentiate into specialized cell types. (bvsalud.org)

Cell Metabolism 2022, 34: 595-614.e14. (yalemedicine.org)

Endothelial stem cells (ESCs) are one of three types of stem cells found in bone marrow. (wikipedia.org)

The further specialized a cell is, the more differentiated it is and as a result it is considered to be more committed to a certain cellular lineage. (wikipedia.org)
Also making up a part of the hematopoietic system are short-term repopulating progenitor cells, which give rise to lineage-specific cell types. (stemcell.com)
Hematopoietic stem/progenitor cells (HSPC) are known to be functionally diverse in terms of their self-renewal potential and lineage output. (lu.se)
therefore, the phenotype was dependent on TET2 loss occurring earlier in the hematopoietic lineage. (aacrjournals.org)
The nature of the stem cell substates and their relationship to commitment to differ- entiate and lineage selection can be elucidated in terms of a landscape picture in which stable states can be defined mathematically as attractors. (lu.se)
This requirement strongly limits the number of solutions or entiation and lineage-specification, programmed cell death, and ``states'' for the system. (lu.se)
2008). Historically, this concept is highlighted by the experi- factors are key intrinsic regulators of these fate decisions and mental phenomenon of lineage reprogramming, for example, that fate choice involves modulating networks of transcription by the conversion of fibroblasts to muscles cells following trans- factors. (lu.se)
Similarly, GATA-1 has been shown to induce lineage switching expression values even if, for simplicity, we assume only ``on'' of committed cells in hematopoiesis, first in cell lines (Kulessa and ``off'' states for each gene. (lu.se)

Physiologic aging in both humans and mice leads to permanent changes in LT-HSC function, such as myeloid-biased hematopoietic output ( Akunuru and Geiger, 2016 ). (biorxiv.org)
The 2016 World Health Organization (WHO) classification of lymphoid neoplasms incorporates genetic data, clinical features, cell morphology, and immunophenotype, all of which have important implications for disease prognosis and management. (msdmanuals.com)

We further provide genetic evidence demonstrating that Lnk controls HSC quiescence and self-renewal, predominantly through Mpl. (jci.org)
This case highlights the importance of genetic counseling in patients with hematopoietic neoplasms with high clinical suspicion for carrying cancer susceptibility gene mutations, which require timely diagnosis and personalized management. (frontiersin.org)
Mikkola's goal, making blood stem cells self-renew in controlled laboratory conditions, would open up a host of new possibilities for treating many blood disorders-among them safer genetic engineering of patients' own blood stem cells. (medicalxpress.com)
Using a viral vector-a specially modified virus that can carry genetic information to a cell's nucleus without causing a disease-the team inserted an active MLLT3 gene into blood stem cells and observed that functional blood stem cells were able to multiply in number at least twelvefold in lab dishes. (medicalxpress.com)
An enlarged HSC pool increases the potential for successive genetic events that drive development of diverse hematopoietic malignancies. (aacrjournals.org)
In addition to these basic issues concerning leukemia pathogenesis, we are devising new diagnostic procedures for detecting and monitoring leukemia patients based on molecular genetic abnormalities in the malignant cells. (stanford.edu)

CD34 is a transmembrane phosphoglycoprotein that was first identified on HSPCs, but its expression is also present on other cell types, such as vascular tissue. (stemcell.com)

The transition of a cell from an inactive-quiescent to an active proliferative state means a massive reorganization of the genome. (eurekalert.org)
Stem cells represent a very small percentage of bone marrow cells, and are normally quiescent. (justia.com)

Balcerek J, Jiang J, Bersenev A , Song Y, Wu C, Tong W. 14-3-3 Regulates the Lnk/JAK2 Pathway In Hematopoietic Stem and Progenitor Cells. (yalemedicine.org)

Although hematopoietic stem cells (HSC) are the best characterized and the most clinically used adult stem cells, efforts are still needed to understand how to best ex vivo expand these cells. (stemcell.com)
Stem cells exist both in embryos and adult cells. (articlecity.com)
Adult stem cells, which are present in small amounts in adult tissue but less adaptable than embryonic stem cells, making their use in medical treatments more challenging. (articlecity.com)
We have shown that KAT6A (MOZ) is essential for the development of haematopoietic stem cells whereas KAT6B (QKF) has an essential role in adult neural stem cells. (edu.au)
We are currently investigating the function of the MYST family, particularly KAT6A and KAT6B, during embryonic development and in adult stem cell populations. (edu.au)
Human adult mobilized CD34+ cells were cultured for 21 days under normoxia (21% O 2 ) or hypoxia (2% O 2 ) in vessels coated with optimized concentrations of Delta1. (confex.com)
1,2 Regarding the origin, they can be classified as embryonic stem cells (ESCs) and adult stem cells (ASCs). (bvsalud.org)

Genomic studies have uncovered silencing TET2 mutations in B-cell and T-cell lymphomas ( 6-8 ), indicating a tumor suppressor role for TET2 in lymphoid malignancies. (aacrjournals.org)
The lab is investigating the role of DNA methyltransferase 3a (DNMT3A) in regulating stem cell function, and how mutations in DNMT3A lead to age-related clonal hematopoiesis and development of hematologic malignancies. (chicagobiomedicalconsortium.org)
A faculty member of the Department of Pediatrics at IU School of Medicine, Dr. Tran is developing a research program to study hematopoietic malignancies. (iu.edu)

The biological properties and clinical potential of stem cells elicit that are generated must not be unduly sensitive to small fluctu- continued scientific, commercial, and public interest. (lu.se)

Additional markers can be used to distinguish HSPC subsets within the CD34+ population and isolate HSPCs with different engraftment abilities and capacities to expand or generate mature blood cells in culture. (stemcell.com)

Dr. Hanna Mikkola, a member of the Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research at UCLA and senior author of the study, has studied blood stem cells for more than 20 years. (medicalxpress.com)

This study demonstrates that the developmental characteristic most consistently associated with telomerase expression is self-renewal potential. (nih.gov)
Progenitor stem cells are committed to differentiating along a particular cell developmental pathway. (wikipedia.org)
However, the results also pinpoint that transposable element RNA is used by the cells during developmental transitions. (eurekalert.org)
Boyer LA, Plath K, Zeitlinger J, Brambrink T, Medeiros LA, et al: Polycomb complexes repress developmental regulators in murine embryonic stem cells. (karger.com)
In addition, my lab studies the process of developmental committment to the B cell lineages, using 7-color flow cytometry on a state-of-the-art 3 laser FACS. (umassmed.edu)

Hematopoietic stem cells (HSC) give rise to all types of blood lineages, including red blood cells (RBC). (lu.se)
Our ongoing studies have found that LT-HSC population, which give rise to all lineages of blood cells, increases in Smurf2-deficient mice, suggesting a beneficial effect of Smurf2 deficiency. (umassmed.edu)

iASPP is a negative regulator of the apoptotic function of p53, and it can enhance the ability of hematopoietic stem cells to self-renew and resist chemo- and radiation therapy. (nature.com)
The researchers subsequently identified the cause of the dysfunctional hematopoiesis: Blood stem cells failed to self-renew as they were forced to divide in response to the inflammatory stimuli. (eurekalert.org)
UCLA scientists have discovered a link between a protein and the ability of human blood stem cells to self-renew. (medicalxpress.com)
In a study published today in the journal Nature , the team reports that activating the protein causes blood stem cells to self-renew at least twelvefold in laboratory conditions. (medicalxpress.com)
They found that the expression of a gene called MLLT3 was closely correlated with blood stem cells' potential to self-renew and that the protein generated by the MLLT3 gene provides blood stem cells with the instructions necessary to maintain its ability to self-renew. (medicalxpress.com)
Dr. Hanna Mikkola studies how blood stem cells are generated during embryonic development and how their ability to self-renew and give rise to differentiated blood cell types is orchestrated by cues from their micro-environment. (lu.se)

One sentence summary Murine hematopoietic stem cells display transcriptional heterogeneity that is quantitatively altered with age and leads to the age-dependent myeloid bias evident after inflammatory challenge. (biorxiv.org)
We demonstrate here that GSK-3 maintains the MLL leukemia stem cell transcriptional program by promoting the conditional association of CREB and its coactivators TORC and CBP with homedomain protein MEIS1, a critical component of the MLL-subordinate program, which in turn facilitates HOX-mediated transcription and transformation. (stanford.edu)

With this raw material, biomedical firms can create stem cell lines that, among other things, aid recuperation via the regeneration of tissue that has been lost or damaged. (articlecity.com)
This indicates that RNA sensing is probably not necessary for hematopoietic regeneration but helps to enhance blood regeneration after chemotherapy," say Thomas Clapes, Aikaterini Polyzou and Pia Prater. (eurekalert.org)
These findings help to better understand the molecular underpinnings of hematopoietic regeneration, especially after chemotherapy. (eurekalert.org)
However, cell proliferation is critical for renewal, repair and regeneration to maintain normal tissue homeostasis and functions. (umassmed.edu)
These cells have been studied for the treatment of periodontitis, bone repair, regeneration of the pulp after necrosis as well as the development of new teeth. (bvsalud.org)

NOD-SCID-Gamma (NSG) mice) and measuring the presence of human blood cells in the blood or bone marrow (BM) after extended periods of engraftment, e.g., 20 weeks or longer. (stemcell.com)
Multiplying blood stem cells in conditions outside the human body could greatly improve treatment options for blood cancers like leukemia and for many inherited blood diseases. (medicalxpress.com)
Other recent studies have identified small molecules-organic compounds that are often used to create pharmaceutical drugs-that help to multiply human blood stem cells in the laboratory. (medicalxpress.com)
Recombinant Human Wnt-3a (Catalog # 5036-WN) induces alkaline phosphatase production by the MC3T3-E1 mouse preosteoblast cell line. (novusbio.com)
Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. (novusbio.com)
This is because stem cells are, basically, the core building blocks of all human cells - which is why they are most prominently a part of human physiology during gestation in the womb. (articlecity.com)
Stem cells are unspecialized cells of the human body. (articlecity.com)
and human embryonic stem cells(hESCs). (sigmaaldrich.com)
Notch activation in human CD34+ hematopoietic stem/progenitor cells (HSPCs) by treatment with Delta1 ligand has enabled clinically relevant ex vivo expansion of short-term HSPCs. (confex.com)
OR14I1 is a receptor for the human cytomegalovirus pentameric complex and defines viral epithelial cell tropism. (umassmed.edu)
Couto P, Rotondi M, Bersenev A , Hewitt C, Nienow A, Verter F, Rafiq Q. Expansion of human mesenchymal stem/stromal cells (hMSCs) in bioreactors using microcarriers: lessons learnt and what the future holds. (yalemedicine.org)
Dysfunctional blood cell development can have fatal consequences on human health, with implications for several key areas of modern medicine, including amongst others the broad research areas of oncology and immunology. (lu.se)
Pyrimidoindole derivatives are agonists of human hematopoietic stem cell self-renewal. (bvsalud.org)

Blood stem cells in the bone marrow provide a lifelong replenishment of the different cell types making up the blood system. (eurekalert.org)
According to experts, the cause of this age-related loss of function of the hematopoietic system is a chronic low-grade inflammatory condition called inflammaging, that only develops in later life and impairs the function blood stem cells. (eurekalert.org)
However, the question that we wanted to answer was whether inflammation and infections in early life can permanently damage blood stem cells and thus promote aging of the blood system," says Mick Milsom of the German Cancer Research Center and the Stem Cell Institute HI-STEM. (eurekalert.org)
This observation in mice contradicts common doctrine: we had previously believed that, after inflammatory challenge, blood stem cells revert into a so-called dormant state that preserves their capacity for self-renewal,' says Milsom, explaining this surprising aspect of his work. (eurekalert.org)
The researchers wondered if maintaining the level of the MLLT3 protein in blood stem cells in lab dishes would be sufficient to improve their self-renewing abilities. (medicalxpress.com)
we also need to ensure that the lab-created blood stem cells can continue to function properly by making all blood cell types when transplanted. (medicalxpress.com)
Our method, which exposes blood stem cells to the small molecules and also inserts an active MLLT3 gene, created blood stem cells that integrated well into mouse bone marrow , efficiently produced all blood cell types and maintained their self-renewing ability. (medicalxpress.com)

The symptoms of acquired aplastic anemia occur as a consequence of the bone marrow failing to produce enough blood cells. (rarediseases.org)

Recently, evidence has been accumulating on the molecular mechanism underlying self-renewal of stem cells. (karger.com)
Dr. Qian is investigating the molecular mechanisms underlying the development of a dominant population of abnormal blood-forming stem cells in Myelodysplastic syndrome (MDS) patients, and searching for new effective therapeutic strategies for the treatment of early stages of MDS patients. (lls.org)
Here we review the currently known cellular and molecular components of the niche for hematopoietic stem cells and their progeny, highlight their heterogeneity and interdependency, and discuss some open questions in the field. (elsevierpure.com)

Under steady-state conditions, mice lacking the inhibitory adaptor protein Lnk harbor an expanded HSC pool with enhanced self-renewal. (jci.org)
No significant alteration of hematopoietic homeostasis and HSC function is observed in young Pus10-/- mice, while aged Pus10-/- mice exhibit mild alteration of hematopoietic homeostasis and HSC function. (haematologica.org)
Importantly, the inflammation in young mice led to persistent changes in the hematopoietic system resembling age-related changes often found in elderly people. (eurekalert.org)
When Mikkola's team used the small molecules, they observed that blood stem cell self-renewal improved in general, but the cells could not maintain proper MLLT3 levels, and they also did not function as well when transplanted into mice. (medicalxpress.com)
Bruggeman SWM, Valk-Lingbeek ME, van der Stoop PPM, Jacobs JJL, Kieboom K, et al: Ink4A and Arf differentially affect cell proliferation and neural stem cell self-renewal in Bmi1-deficient mice. (karger.com)
In aged mice, the self-renewal capacity of long-term hematopoietic stem cells (LT-HSC) diminishes. (umassmed.edu)

Cooperation between both Wnt/ß-catenin and PTEN/PI3K/Akt signaling promotes primitive hematopoietic stem cell self-renewal and expansion. (stowers.org)
Mediates ex vivo expansion of cord blood CD34+ hematopoietic stem and progenitor cells (Huang et al. (stemcell.com)
As people age, they can acquire somatic mutations that lead to clonal expansion within their population of regenerating hematopoietic stem cells, which in turn can lead to hematological cancer and coronary heart disease. (genomeweb.com)
We find that NFATC1 governs FLT3 ITD -driven precursor cell expansion and transformation, causing a fully penetrant lethal AML. (biomedcentral.com)

However, the cell-damaging effect of chemotherapy affects cancer cells but also in principle many other cell types, including cycling blood cells. (eurekalert.org)
Researchers from the MPI of Immunobiology and Epigenetics, together with colleagues from the University of Freiburg, Lyon, Oxford, and St Jude Children's Research Hospital in Memphis, now discovered that hematopoietic stem cells make use of RNA molecules from junk DNA sections to enhance their activation after chemotherapy. (eurekalert.org)
Hematopoietic stem cells respond to chemotherapy by starting proliferating. (eurekalert.org)
It is interesting to think that cells make use of transposable elements or other repetitive RNAs to finetune and adapt whenever they need to change their state for example after stress, like chemotherapy or even after physiological stress signals like development or aging," says Eirini Trompouki. (eurekalert.org)
They can also be used for increasing white blood cell survival and for chemotherapy. (justia.com)
This is defined by their ability to repopulate an individual whose hematopoietic system has been destroyed by radiation or chemotherapy. (justia.com)
After harvesting, the patient is given high dose chemotherapy or radiotherapy and the bone marrow function is reconstituted by infusion of the cells harvested earlier. (justia.com)
The use of high-dosage chemotherapy or radiotherapy for bone marrow ablation requires subsequent incorporation of hematopoietic stem cells into the patient, in which case prior harvesting of such cells is required. (justia.com)

In a series of assays, they found that individuals with this variant had decreased TET2 expression and that the reduction of TET2 activity was linked to increased self-renewal and proliferation of hematopoietic stem cells. (genomeweb.com)

CD4 + T cells contribute to the remodeling of the microenvironment required for sustained tumor regression upon oncogene inactivation. (nature.com)
Relationship between hematogenous tumor cell dissemination and cellular immunity in DCIS patients. (nature.com)
Many tumor cell lines and experimental tumors respond to HDAC inhibition. (aacrjournals.org)
The study is an interdisciplinary effort to generate safe and effective chimeric antigen receptor (CAR)-T cell therapy for cancer that will be done by doing a deep-dive into understanding the relationship between the metabolism and anti-tumor functions of CAR-T cells. (uclahealth.org)
These changes can be inherited and are, therefore, found in every cell, but more often, they are somatically acquired and restricted to tumor cells. (medscape.com)

In addition to its role in megakaryocyte production, signaling initiated by thrombopoietin (TPO) activation of its receptor, myeloproliferative leukemia virus protooncogene (c-Mpl, or Mpl), controls HSC homeostasis and self-renewal. (jci.org)
Recent study showed that iASPP could impact the proliferation and apoptosis of leukemia cells by interacting with FHL2 . (nature.com)
Jude CD, Gaudet JJ, Speck NA, Ernst P. Leukemia and hematopoietic stem cells: balancing proliferation and quiescence. (nature.com)
FHL2 interacts with iASPP and impacts the biological functions of leukemia cells. (nature.com)
We have reported that the expression of BMI1 , a member of PcG, in hematopoietic stem cells or progenitor cells predicts the prognosis of patients with MDS and progression to acute leukemia. (karger.com)

The department focuses on research that aims to develop gene therapy for hematopoietic cells using viral vectors. (lu.se)

We observed enhanced progenitor cell activity within the CD34+ cell population treated with Delta1 in hypoxia, but the benefits provided by low-oxygen cultures were most notable in the primitive HSC compartment. (confex.com)
3,4 The zygote and cells derived from the first two cellular divisions constitute the most primitive cells (totipotent cells) that are capable of forming the embryo and the embryonic annexes (e.g. placenta, amniotic membranes etc). (bvsalud.org)

02:47 and red blood cells in vitro. (yale.edu)

Resolving the Pathogenesis of Anaplastic Wilms Tumors through Spatial Mapping of Cancer Cell Evolution. (lu.se)

In acquired aplastic anemia, an almost complete absence of hematopoietic stem cells results in low levels of red and white blood cells and platelets (pancytopenia). (rarediseases.org)
Red and white blood cells and platelets are formed in the bone marrow. (rarediseases.org)
Red blood cells deliver oxygen to the body's organs, white blood cells help in fighting infections, and platelets form clots to stop bleeding. (rarediseases.org)
Other cell types derived from hematopoietic stem cells include platelets and erythrocytes. (justia.com)

Inflammatory exposure drives long-lived impairment of hematopoietic stem cell self-renewal activity and accelerated aging. (eurekalert.org)

Mesenchymal stem cells (MSCs) are ASCs, and were first described in 1966 by Friedenstein et al. (bvsalud.org)

The researchers homed in on a set of about two dozen likely causal variants, which they hypothesized affects a TET2 enhancer in hematopoietic stem cells. (genomeweb.com)
Since the initial discovery of the self-renewing properties of stem cells in the early 1960s - pioneered by a small team of researchers at the Ontario Cancer Institute [3] - the understanding of the power and diversity of stem cells has increased rapidly. (articlecity.com)

Anatomy50

Organisms14

Diseases27

Chemicals and Drugs56

Analytical, Diagnostic and Therapeutic Techniques and Equipment54

Psychiatry and Psychology3

Phenomena and Processes48

Disciplines and Occupations3

Anthropology, Education, Sociology and Social Phenomena2

Information Science3

Named Groups3

Health Care7

Differentiation35

Aged hematopoietic stem and progenitor cells1

Transplantation13

Progenitors3

Pathways4

Regulate2

HSCs17

Pluripotent11

Leukemic2

Genes14

Regulation6

Biology8

Enhances4

Embryonic stem2

Tissues7

Differentiate6

20221

Found in bone marrow1

Lineage8

20162

Genetic6

CD341

Quiescent2

Regulates1

Adult7

Malignancies3

Clinical1

HSPC1

Regenerative Medicine1

Developmental5

Lineages2

Self-renew6

Transcriptional2

Regeneration5

Human13

Blood stem cells7

Bone marrow failing to1

Molecular3

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Assays1

Tumor5

Leukemia5

Viral1

Primitive2

Vitro1

Tumors1

Platelets4

Long-lived1

Mesenchymal1

Researchers2