• Structural studies of ribosomal RNA based on cross-analysis of comparative models and three-dimensional crystal structures (PhD thesis). (wikipedia.org)
  • Specific tertiary structure types are observed for transfer RNA (tRNA), ribosomal RNA (rRNA), small nuclear RNA (snRNA), certain introns, and ribozymes. (biosyn.com)
  • For ribosomal RNA (rRNA) depletion from human, mouse, or rat samples, use the NEBNext rRNA Depletion Kit (Human/Mouse/Rat) ( NEB #E6310 ). (neb.com)
  • The most commonly used molecular approach for exploring microbial diversity is based on the PCR amplification and sequencing of genes encoding small subunit ribosomal RNA (16S rRNA) directly from environmental sources [ 16 ]. (biomedcentral.com)
  • In bacteria , a single RNA polymerase produces the primary transcript precursors for all three major classes of RNA: messenger RNA (mRNA), ribosomal RNA (rRNA), and transfer RNA (tRNA). (basicmedicalkey.com)
  • Ribosomes are made mostly of rRNA (ribosomal ribonucleic acid), and bacterial cells contain more than 50 associated proteins, whereas plant and animal cells contain 80 associated proteins. (visiblebody.com)
  • Linezolid binds to the 23S ribosomal RNA of the 50S subunit of the bacterial ribosome and prevents the formation of a functional 70S initiation complex which is an essential component of the bacterial translation process. (com.bd)
  • Aminoglycosides (AG) are antibiotics that lower the accuracy of protein synthesis by targeting a highly conserved RNA helix of the ribosomal A-site. (st-andrews.ac.uk)
  • Our new NEBNext Ultra II RNA kits have streamlined, automatable workflows and are available for directional (strand-specific, using the "dUTP method") and non-directional library prep and are compatible with poly(A) mRNA enrichment or rRNA depletion. (neb.com)
  • For depletion of bacterial rRNA from bacterial samples, use the NEBNext rRNA Depletion Kit (Bacteria) ( NEB #E7850 ). (neb.com)
  • Herein we report the synthesis of 6-fluorosisomicin, the first 6-fluorinated aminoglycoside, which specifically interacts with the protozoal cytoplasmic rRNA A-site, but not the bacterial A-site, as evidenced by X-ray co-crystal structures. (st-andrews.ac.uk)
  • The cleared lysate is then loaded onto the anion-exchange tip where plasmid DNA selectively binds under appropriate low-salt and pH conditions. (qiagen.com)
  • Specific sequences on DNA, called promoters , determine where the RNA polymerase binds and how frequently it initiates transcription. (basicmedicalkey.com)
  • Here we show that staphylococcal phages produce a structured RNA transcribed from the terminase subunit genes, termed CBASS-activating bacteriophage RNA (cabRNA), which binds to a positively charged surface of the CdnE03 cyclase and promotes the synthesis of the cyclic dinucleotide cGAMP to activate the CBASS immune response. (bvsalud.org)
  • As the mammalian cyclase OAS1 also binds viral double-stranded RNA during the interferon response, our results reveal a conserved mechanism for the activation of innate antiviral defence pathways. (bvsalud.org)
  • A complementary RNA sequence that binds to a naturally occurring (sense) mRNA molecule, thus blocking its translation. (genomicglossaries.com)
  • RNA polymerase inhibition studies. (bjzblg.com)
  • 1 It strongly inhibits Escherichia coli RNA polymerase and plant chloroplast RNA polymerase. (bjzblg.com)
  • 4 Tagetin Inhibitor complements the activity of α-amanitin, a potent and selective inhibitor of eukaryotic RNA polymerase II. (bjzblg.com)
  • Only known specific inhibitor of RNA polymerase III from many nonplant eukaryotes. (bjzblg.com)
  • Specific inhibition of chloroplast RNA polymerase of many plants. (bjzblg.com)
  • One unit (30 pmol) of Tagetin Inhibitor results in 50% inhibition of 1 unit of E. coli RNA Polymerase Holoenzyme under standard assay conditions using phage T7 DNA as a template. (bjzblg.com)
  • Tagetin RNA Polymerase Inhibitor is provided in water at a concentration of 20 U/µl (600 µM). (bjzblg.com)
  • Tagetin Inhibitor is functionally tested with EPICENTRE's E. coli RNA Polymerase Holoenzyme and is free of detectable RNase and DNase activities. (bjzblg.com)
  • Tagetin Inhibitor activity on E. coli RNA Polymerase. (bjzblg.com)
  • Each gel lane shows products of a standard transcription reaction using a bacteriophage template, 1 U of E. coli RNA Polymerase Holoenzyme, and varying amounts of Tagetin Inhibitor. (bjzblg.com)
  • 50% inhibition of transcription is seen at 1 U Tagetin Inhibitor per unit of E.coli RNA Polymerase. (bjzblg.com)
  • 87(5): 1663-1667] Specifically, the investigators started by priming whole cerebellar RNA with a synthetic oligonucleotide containing a T7 RNA polymerase promoter sequence. (genomicglossaries.com)
  • After second- strand cDNA synthesis, T7 RNA polymerase was used to generate aRNA. (genomicglossaries.com)
  • Fidaxomicin is different, in that its actions are bactericidal, and it works by binding to the switch regions of RNA polymerase. (naplexstudyguide.com)
  • This was related to an increase in TF messenger RNA (mRNA) stability. (imperial.ac.uk)
  • Ribonucleoprotein complex immunoprecipitation and biotinylated RNA pull-down assays demonstrated that PARP-14 forms a complex with the mRNA-destabilizing protein tristetraprolin (TTP) and a conserved adenylate-uridylate-rich element in the TF mRNA 3′ untranslated region. (imperial.ac.uk)
  • m7G-Seq utilizes chemical reduction and deamination to selectively convert the m7G site into the basic site, and by reverse transcription enzyme to successfully detect the m7G signal within the mRNA (11). (beds.ac.uk)
  • Because alternative RNA splicing allows different types of mRNA molecules to be created from a single gene, it generates the diversity of protein function and structure that is essential to complex organisms. (genomicglossaries.com)
  • RNA-like oligonucleotides that are complementary to a portion of a target mRNA molecule. (genomicglossaries.com)
  • More specifically, antisense oligonucleotides that are useful as reagents for target validation , or as drugs, are engineered molecules that differ from natural RNA but that have a base sequence that is recognized as being complementary to a very specific mRNA sequence. (genomicglossaries.com)
  • The defect also acted as a molecular handle, which enabled the researchers to introduce a detection unit into the sensor, that can be adapted to a target molecule for the purpose of identifying a specific viral or bacterial protein. (photonics.com)
  • The interaction between the detection unit and a bacterial or viral molecule affects the fluorescence of the nanotubes, causing their brightness to increase or decrease. (photonics.com)
  • In neonates, the inflammation is usually caused by congenital viral, bacterial, or protozoal infections. (medscape.com)
  • In insects, we compared sequences of small and long RNAs, demonstrating that viral sequences are enriched in the small RNA fraction. (cnrs.fr)
  • We also noted that the small RNA size profile is a unique signature for each virus and can be used to identify novel viral sequences without known relatives in reference databases. (cnrs.fr)
  • We also show that the small RNA profile could be used to infer viral tropism for ovaries among other aspects of virus biology. (cnrs.fr)
  • Currently, there are three basic types of tests to determine if an individual has been infected with SARS-CoV-2: viral nucleic acid (RNA) detection, viral antigen detection, and detection of antibodies to the virus. (medscape.com)
  • It selectively inhibits bacterial protein synthesis via a mechanism of action different from that of other antibacterial agents. (com.bd)
  • Tetracyclines are a class of antibiotics that inhibit bacterial protein synthesis by binding to the 30s subunit of their ribosomes and preventing tRNA from binding. (osmosis.org)
  • Abundant RNA species such as rRNAs and certain mRNAs can interfere with detection and analysis of biologically relevant RNAs of interest. (neb.com)
  • The detection of RNA modifications is fundamental to the study of m7G modifications in the regulation of gene expression. (beds.ac.uk)
  • With SARS-CoV-2 as the model, the proposed method (RPA-TeaPNA-LFA) efficiently eliminated the false-positives, exhibiting a lowest detection concentration of 6.7 copies/µL of RNA and 90 copies/µL of virus. (bvsalud.org)
  • A 2019 study conducted in Puerto Rico showed that the 95th percentile for the time until the loss of Zika virus RNA detection was 54 days in serum, 34 days in urine, and 81 days in semen. (medscape.com)
  • The Acclaro reference libraries currently support detection of protein, phenol, guanidine HCl, and RNA in dsDNA samples. (thermofisher.com)
  • They also support detection of protein, phenol, guanidine isothiocyanate, and dsDNA in RNA samples. (thermofisher.com)
  • Additionally, our results suggest that virus detection utilizing small RNAs can also be applied to vertebrates, although not as efficiently as to plants and insects. (cnrs.fr)
  • Bacterial ribosomes are made up of a 50S subunit and a 30S subunit which combine to form a 70S ribosome. (osmosis.org)
  • glnA RNAs are found in the presumed 5' untranslated regions of genes encoding multiple classes of protein that are involved in nitrogen metabolism. (wikipedia.org)
  • It was demonstrated that glnA RNAs correspond to glutamine-binding riboswitches, i.e., they sense glutamine concentrations in order to measure overall nitrogen availability, and regulate the downstream genes appropriately. (wikipedia.org)
  • Genes are transcribed by enzymes called RNA polymerases that generate a single-stranded RNA identical in sequence (with the exception of U in place of T) to one of the strands of the double-stranded DNA. (basicmedicalkey.com)
  • Eukaryotic genes are transcribed in the nucleus by three different RNA polymerases , each principally responsible for one of the major classes of RNA. (basicmedicalkey.com)
  • This is measured by a different assay, Tn-seq, which takes advantage of transposon insertion to selectively inactivate genes in the bacterial genome. (michaelchimenti.com)
  • Synthetic antisense RNAs are used to effect the functioning of specific genes for investigative or therapeutic purposes. (genomicglossaries.com)
  • 3 With both eukaryotic and prokaryotic RNA polymerases, the degree of inhibition is template-dependent. (bjzblg.com)
  • RNA polymerases differ from DNA polymerases in that they can initiate the synthesis of new strands in the absence of a primer. (basicmedicalkey.com)
  • The transcribed region of a gene contains the template for synthesis of an RNA, which begins at the start point. (basicmedicalkey.com)
  • It is not really understood why this happens, but the authors theorize that having the bottom half of the pathway under transcriptional control allows the bacterial to control flux into Trp synthesis and other AA sub-pathways while always maintaining a stable supply of the starting point intermediates (the product of SP1374) through reversible, end product-regulated biosynthesis. (michaelchimenti.com)
  • Her team determined that Plasmodium species, including P. falciparum, one of five protozoa that cause malaria and the one that accounts for more than 90% of mortality associated with malaria, relies exclusively on pyrimidine biosynthesis to provide precursors for DNA and RNA synthesis, so blocking pyrimidine biosynthesis can selectively kill the parasite without damaging other critical cells. (asbmb.org)
  • Tetracyclines are antimicrobial antibiotics that inhibit bacterial ribosomes which are the organelles that make proteins. (osmosis.org)
  • The Phillips lab explores antimalarial compounds that selectively inhibit the DHODH parasite. (asbmb.org)
  • Transport RNA, or tRNA, carrying different amino acids can bind to these codons with their matching anticodons. (osmosis.org)
  • With advances in UV-Vis software, researchers can now selectively quantify nucleic acid absorbance in the presence of chemical and nucleic acid contaminants . (thermofisher.com)
  • Krishnamurthy SR, Janowski AB, Zhao G, Barouch D and Wang D. Hyperexpansion of RNA bacteriophage diversity. (wustl.edu)
  • Alfred Hershey and Martha Chase performed several experiments indicating that DNA was the genetic material in a bacterial virus called T2 bacteriophage. (micrordt.com)
  • Nucleic acids, such as antisense oligonucleotides (AONs) and small interfering RNAs (siRNAs) have enormous potential as therapeutics for life-threatening diseases because in principle, any problematic gene can be targeted with high efficacy and sequence-specificity. (grstiftung.ch)
  • We describe here a new class of caged morpholino oligonucleotides that can be activated by the bacterial nitroreductase NfsB. (cdc.gov)
  • This approach involves the intramolecular cross-linking of 5′ amine- and 3′ disulfide-modified MO oligonucleotides using appropriately functionalized tethers, generating macrocyclic structures that conformationally resist RNA hybridization. (cdc.gov)
  • Bioinformatic algorithms allowing predicting of biomolecular folding for proteins, peptides, and RNAs, even though sometimes successful, have all their limitations. (biosyn.com)
  • RNA molecules in solution may adopt secondary structures that are only partially determined by thermodynamics since RNA molecules can undergo conformational changes during interaction with other RNAs, RNA binding proteins or RNA binding peptides. (biosyn.com)
  • Since RNA-binding proteins (RBPs) are key players in the post-transcriptional regulation of gene expression precise knowledge of their binding sites is critical for determining their molecular function and for understanding their roles in cell development and disease. (biosyn.com)
  • RNA codon misreading [formation of toxic, non functional proteins]. (mindmeister.com)
  • Due to their structure, they can selectively bind to proteins," researcher Justus Metternich said. (photonics.com)
  • Since these proteins are different, we can created medications that selectively interfere with the bacterial ones. (osmosis.org)
  • 2 Plant nuclear RNA polymerases I, II, and III are much less sensitive to Tagetin Inhibitor. (bjzblg.com)
  • Phage-encoded RNA polymerases such as SP6 and T7 are also relatively insensitive. (bjzblg.com)
  • The research, published March 24 in PLOS Biology, has identified 122 new types of RNA bacteriophages in diverse ecological niches, providing an opportunity for scientists to define their contributions to ecology and potentially to exploit them as novel tools to fight bacterial infections, particularly those that are resistant to antibiotics. (wustl.edu)
  • RNA bacteriophages have been shown to infect gram-negative bacteria, which have become increasingly resistant to antibiotics and are the source of many infections in health-care settings. (wustl.edu)
  • It is effective against Gram-positive and Gram-negative bacterial infections. (canadianpillschoice.net)
  • Lincocin is an antibiotic used to treat a wide variety of bacterial infections. (canadianpillschoice.net)
  • Fidaxomicin is a narrow-spectrum drug that selectively inhibits Clostridium difficile infections. (naplexstudyguide.com)
  • Erythromycin is prescribed for treatment of bacterial infections. (world-family-health7.pw)
  • Cryo-electron microscopy reconstructions of an NSP2-RNA complex reveal a highly conserved acidic patch on the CTR, which is poised toward the bound RNA. (cam.ac.uk)
  • The fact that RNAs from both motifs selectively bind glutamine supports this hypothesis, but detailed structural data is not yet available. (wikipedia.org)
  • DNA purification procedure consists of four steps and utilizes spin minicolumns with membranes which efficiently and selectively bind nucleic acids. (blirt.eu)
  • Despite phage replication in these matrices, lysis of the bacteria was slower than phage propagation, and no reduction of the bacterial population was observed. (bvsalud.org)
  • Eventually, we'd like to manipulate that dynamic to use phages to selectively kill particular bacteria. (wustl.edu)
  • Over the last decades, a variety of RNA methods have been developed for the study of RNA-DNA, RNA-RNA, and RNA-protein interactions including RNA complexes with ligand molecules. (biosyn.com)
  • RNA molecules are functionally diverse and involved in many cellular processes such as catalysis, ligand binding, and protein recognition. (biosyn.com)
  • RNA molecules are structural flexible and can adopt different structures. (biosyn.com)
  • RNAs are single-stranded molecules and the unlinked monomer building blocks are known as nucleotides. (biosyn.com)
  • RNA molecules are generally folded into compact and defined tertiary structures. (biosyn.com)
  • Rotavirus genomes are distributed between 11 distinct RNA molecules, all of which must be selectively copackaged during virus assembly. (cam.ac.uk)
  • RNA molecules which hybridize to complementary sequences in either RNA or DNA altering the function of the latter. (genomicglossaries.com)
  • In molecular biology, generalized transduction is routinely used as a technique to manipulate and construct bacterial strains. (bvsalud.org)
  • The DNA strand that directs the sequence of nucleotides in the RNA by complementary base pairing is the template strand. (basicmedicalkey.com)
  • Interestingly, our data indicate that tethering of 200-250 nucleotides long sequence to either 5′ or 3′ ends of guide RNA did not significantly affect Cas9 cleavage activity. (nsf.gov)
  • Helixyte Green dsDNA Quantitation Assay Kit can be used for selectively detecting as little as 25 pg/ml of dsDNA in the presence of ssDNA, RNA, and free nucleotides. (aidstar-one.com)
  • High-throughput sequencing refers to the use of antibody immunoprecipitation or chemical methods to accurately locate the site of m7G modification in RNA. (beds.ac.uk)
  • CRISPR‐Cas9 has been shown to be a valuable tool in recent years, allowing researchers to precisely edit the genome using an RNA‐guided nuclease to initiate double‐strand breaks. (nsf.gov)
  • CRISPR-Cas12a is an RNA-guided, programmable genome editing enzyme found within bacterial adaptive immune pathways. (nsf.gov)
  • To investigate how the relative potency of cis- versus trans-DNase activity affects Cas12a-mediated genome editing, we first used structure-guided engineering to generate variants of Lachnospiraceae bacterium Cas12a that selectively disrupt trans-activity. (nsf.gov)
  • The advent of large-scale genome sequencing has helped scientists identify DNA bacteriophages in the human gut, skin and blood as well as in the environment, but few researchers have looked for RNA bacteriophages in those samples (doing so requires that RNA be isolated from the samples and then converted back to DNA before sequencing). (wustl.edu)
  • A new paper in Cell Reports utilizes RNA-seq and Tn-seq (the "tn" in tn-seq stands for transposon) to map the transcriptional and fitness changes in bacterial gene networks in response to stressors, like nutrient depletion and antibiotics. (michaelchimenti.com)
  • The discovery of AGs that selectively target the eukaryotic ribosome, but lack activity in prokaryotes, are promising as antiprotozoals for the treatment of neglected tropical diseases, and as therapies to read-through point-mutation genetic diseases. (st-andrews.ac.uk)
  • However, bacterial resistance to macrolides is relatively common, often caused by mutations within the ribosome that prevent the macrolide from binding. (naplexstudyguide.com)
  • RNA which contains an intron sequence that has an enzyme- like catalytic activity. (genomicglossaries.com)
  • These investigators selectively destroyed constituents in purified extracts of virulent pneumococci (S cells), using enzyme that would hydrolyze DNA, RNA, or protein. (micrordt.com)
  • NfsB is a dimeric flavoprotein enzyme that has broad electron acceptor specificity 15 and has been used in conjunction with prodrugs to selectively ablate cells in vertebrates. (cdc.gov)
  • The respective dispositions of 6-fluorosisomicin within the bacterial and protozoal A-sites reveal that the fluorine atom acts only as a hydrogen-bond acceptor to favorably interact with G1408 of the protozoal A-site. (st-andrews.ac.uk)
  • Shown is a micrograph image of RNA bacteriophages attached to part of the bacterium E. coli. (wustl.edu)
  • During the 1950s, S aureus and E coli were the most common bacterial pathogens among neonates in the United States. (medscape.com)
  • Bacterial organisms with increased antibiotic resistance have also emerged and have further complicated the management of neonatal sepsis. (medscape.com)
  • Researchers at Stanford University used such a method to produce amplified heterogeneous populations of RNA from limited quantities of cDNA. (genomicglossaries.com)
  • More recently, another group of researchers reported that they had developed a process for optimizing low- abundance RNA, by combining aRNA amplification with template- switching. (genomicglossaries.com)
  • The original proposal of a riboswitch function was based on the above evidence that glnA RNAs are cis-regulatory, as well as the moderate structural complexity in the three-stem junction of the glnA RNA motif that is comparable to the structures of other known riboswitches. (wikipedia.org)
  • A possible structural resemblance was observed between the glnA RNA motif and the Downstream-peptide motif. (wikipedia.org)
  • Structural basis of rotavirus RNA chaperone displacement and RNA annealing. (cam.ac.uk)
  • Unexpectedly, structural proteomics data revealed that the CTR does not directly interact with RNA, while accelerating RNA release from NSP2. (cam.ac.uk)
  • The antimicrobial wasp Polybia-MP1 peptide selectively kills bacterial cells! (biosyn.com)
  • Using this approach, neuroscientists can now selectively activate and de-activate specific neurons in the brain, allowing them to map brain circuits and investigate how disruption of those circuits causes disease. (mit.edu)
  • Few participants had detectable Zika virus RNA in saliva or vaginal secretions. (medscape.com)
  • Here, we developed a sequence-independent strategy based on virus-derived small RNAs produced by the host response, such as the RNA interference pathway. (cnrs.fr)
  • The characterization of RNA and RNA interactions is closely related to transcription, for example, gene expression levels are investigated within a biological context. (biosyn.com)
  • The transcriptional response measures changes in gene expression as measured by RNA-seq. (michaelchimenti.com)
  • With the accurate identification of m7G modifications within RNAs, their functional roles in the regulation of gene expression and different physiological functions have been revealed. (beds.ac.uk)
  • Epigenetics refers to heritable changes in gene expression without changing the nucleotide sequence of the gene, including DNA methylation, histone modifications, and RNA modifications. (beds.ac.uk)
  • In this project, we propose to develop a prodrug platform of a polynucleotide conjugate that will be activated selectively in the colon lumen by bacterial enzymes which are present in the gut flora. (grstiftung.ch)
  • A gene is a segment of DNA that functions as a unit to generate an RNA product or, through the processes of transcription and translation, a polypeptide chain. (basicmedicalkey.com)
  • Functional screening of PARP-14-deficient macrophages mice revealed that PARP-14 deficiency leads to increased TF expression and functional activity in macrophages after challenge with bacterial lipopolysaccharide. (imperial.ac.uk)
  • These results indicate that FOS selectively modulates bacterial pathogenicity by interfering with different signaling pathways. (ugr.es)
  • Several RNA depletion products are available to selectively degrade and remove unwanted RNAs from your sample. (neb.com)
  • Not only is m7G involved in the normal physiological metabolism of RNA, but recent research also shows that m7G and related regulators appear to be considerably dysregulated in tumors [ 12 ]. (beds.ac.uk)
  • For single cell and ultra-low input RNA, our separate template-switching-based workflow generates full length cDNAs, followed by conversion to sequence-ready libraries using the Ultra ™ II FS workflow, that incorporates enzymatic DNA fragmentation. (neb.com)
  • This likely occurs through sequence-specific RNA interactions facilitated by the RNA chaperone NSP2. (cam.ac.uk)
  • The mapping of RNA-protein or RNA-RNA interactions by protein pull-down or affinity pull-down methods allow studying RNA structures, as well as RNA-protein, and RNA-RNA interactions. (biosyn.com)
  • During transcription , a specific gene on the DNA is "read" and a copy is made called a messenger RNA, which is like a blueprint with instructions on what protein to build. (osmosis.org)
  • OPTR ) announced today that the U.S. Food and Drug Administration's (FDA) Anti-Infective Drugs Advisory Committee (AIDAC) recommended that the FDA approve Optimer's investigational antibiotic DIFICID™ (fidaxomicin) for the treatment of patients with Clostridium difficile infection (CDI), a bacterial infection in the lining of the gut that can cause severe diarrhea, colitis and in some cases death. (prnewswire.com)
  • How bacterial cyclases recognize phage infection is not known. (bvsalud.org)
  • Consistent with these in vitro findings, phage treatment did not reduce bacterial burdens in a neutropenic mouse S. aureus thigh infection model. (bvsalud.org)
  • Chlamydia trachomatis is the leading cause of both bacterial sexually transmitted infection and infection-derived blindness world-wide. (plos.org)
  • The combination of different biochemical methods with computational modelling allows scientists to gain insight into molecular processes in which RNA is involved. (biosyn.com)
  • RNA is a long, polymer of ribonucleoside monophosphate molecular units or building blocks joined together by phosphodiester linkages. (biosyn.com)
  • Molecular formula of common RNA modifications. (beds.ac.uk)
  • Lots of DNA bacteriophages have been identified, but there's an incredible lack of understanding about RNA bacteriophages," explained senior author David Wang, PhD, associate professor of molecular microbiology. (wustl.edu)
  • QIAfilter Cartridges , provided in QIAfilter, HiSpeed, and EndoFree Plasmid Kits, are special filter units designed to replace centrifugation following alkaline lysis of bacterial cells. (qiagen.com)
  • Our previous RNA-seq library preparation required a minimum input of 500 ng total RNA, but the high efficiency of the NEBNext Ultra II kit has allowed us to reduce this to either 100 ng for a standard pipeline, or down to just 5 ng for a low input option, enabling RNA-seq to be carried out from just 5,000-10,000 cells. (neb.com)
  • We selectively assess the editing efficiency of 56 LbCas12a variants in human cells, identifying an optimal LbCas12a variant (RVQ: G146R/R182V/E795Q) with the most robust editing activity. (nsf.gov)
  • After centrifugation, radioactivity in the supernatant (where the virus remained) versus the bacterial cells in the pellet was determined. (micrordt.com)
  • Applications such as bacterial culture growth, kinetics, and chemometric applications such as QC of liquid polymers are also supported. (thermofisher.com)
  • The primary transcripts are modified and trimmed to produce the mature RNAs. (basicmedicalkey.com)
  • The nucleus stores information in the form of DNA and controls the activities of the cell by selectively transcribing RNA, which exits via the nuclear pores. (visiblebody.com)
  • Do you have ever-decreasing amounts of input RNA? (neb.com)
  • To address these challenges, our next generation of RNA library prep kits have been reformulated at each step, resulting in several fold higher yields of high quality libraries, enabling use of lower input amounts and fewer PCR cycles. (neb.com)