Secretion regulates loci genes bacterial. Medical search. Frequent questions

BipA, a ribosomal binding GTPase and prolific regulator of EPEC virulence, transcriptionally regulates Ler from an upstream position where it also regulates other genes. (wikipedia.org)
At present it is assumed that the relationship between an individual's susceptibility and bacterial virulence determines the balance between tolerance of invading pathogens and the mounting of an immune response, which in turn dictates the course of infection and subsequent recurrence. (ncl.ac.uk)
The bacterially produced tryptophan derivative indole is known to influence intestinal barrier function, and has also been shown to regulate virulence gene expression in EHEC. (grantome.com)
Accordingly, the specific aims of this application are: 1) In vitro mechanistic studies on virulence gene regulation by tryptophan derivatives. (grantome.com)
3) Serotonin regulation of bacterial virulence in the murine GI tract. (grantome.com)
Analysis of virulence related gene expression and computational simulations of pivotal proteins involved in pathogenesis demonstrate that myricetin downregulates the saeR global regulator and interacts with sortase A and α-hemolysin. (nature.com)
Unlike antibacterials that aim to inhibit cell growth, antivirulence therapies are based on the inhibition of bacterial virulence. (nature.com)
Additionally, genes conferring virulence and antibiotic resistance were identified amongst the plasmids. (frontiersin.org)
Depending on the genes it carries, it could act as a fitness factor to the host, or serve as a virulence factor by transforming the host into a pathogen. (frontiersin.org)
As with bopE and bopC, no roles were observed for the BsaN-regulated effector candidate loci BPSS1513-1514 in cell-based virulence assays. (mirnaarray.com)
BsaN orchestrates the temporal and spatial expression of virulence determinants during progression through the intracellular lifecycle, EPZ5676 mw promoting endosome escape and possibly evasion of autophagy through activation of T3SS3 effector loci, facilitating cell-cell spread by activation of T6SS1 and the bim intracellular motility loci, and suppressing cellular immunity via the action of the TssM ubiquitin hydrolase. (mirnaarray.com)
The gastric cancer phenotype was associated with the presence of babA and genes in the cag pathogenicity island, one of the major virulence determinants of H. pylori , as well as non-synonymous variations in several less well-studied genes. (biomedcentral.com)
2020 " Thiosulfinate Tolerance Is a Virulence Strategy of an Atypical Bacterial Pathogen of Onion. (baltruslab.com)

The locus of enterocyte effacement (LEE) in EHEC encodes a type three secretion system and effector proteins, all of which are essential for bacterial adherence to host cells. (nih.gov)
Through CpxA, both serotonin and indole decrease expression of the locus of enterocyte effacement (LEE) in EHEC, which encodes a type three secretion system (T3SS), effectors and an adhesin necessary for EHEC to form lesions on enterocytes leading to intestinal disease. (grantome.com)

We have studied how bacterial species such as the pathogen Salmonella enterica maintain a discrete number of flagellar per cell during cell growth and division. (ncl.ac.uk)
The resulting data provide new insights into how Zn2Cys6 TF genes regulate important traits during the infection cycle of this rice blast pathogen. (prelekara.sk)
Listeria monocytogenes is a Gram-positive, facultative intracellular bacterial pathogen that can cause severe disease in humans, other mammals and birds [ 1 ]. (biomedcentral.com)
Chlamydia trachomatis is an obligate intracellular human pathogen that exhibits stage-specific gene transcription throughout a biphasic developmental cycle. (plos.org)

Significant changes in gene expression of proteins that mediate oxidative metabolism, such as cytochrome d ubiquinol oxidase-encoding genes, cydA and cydB , were detected. (microbiologyresearch.org)
Finally, genes encoding putative regulatory proteins such as extracellular function (ECF) sigma factors as well as small proteins had elevated expression levels in the presence of oxygen. (microbiologyresearch.org)
More than half of all human genes are alternatively spliced, so it is expected that alternative pre- mRNA splicing will be responsible for the identification of more than 90 percent of all human proteins. (genomicglossaries.com)
Downstream genes involved in type VII secretion systems, cell wall synthesis (polyketide biosynthesis proteins), and iron uptake were changed in response to the intracellular environment of macrophages. (biomedcentral.com)
The ank gene product is one of several cell membrane proteins, including ectonucleoside triphosphate pyrophosphohydrolase enzymes and alkaline phosphatase, that regulate extracellular inorganic pyrophosphate levels and thereby regulate mineralization. (biomedcentral.com)
In the RT-qPCR, expression ratio demonstrated that the α-amylase gene of two different larval stages grown on both proteins treated leaves had significantly differentiated expression and was up-regulated in third instar larvae and down-regulated in fourth instar larvae compared to control. (potatobeetle.org)
Furthermore, the transcriptional responses of B5 at different temperature by using rice straw as sole carbon sources were also analyzed, based on which the mechanism of lignocellulose degradation at high temperature was revealed that B5 could resist the heat by up-regulating the heat shock proteins (HSP) and then secrete various Carbohydrate-Active enzymes (CAZymes) to realize energy balance. (researchsquare.com)

When compared with historic strains, recent isolates showed increased expression of flagellar genes and genes involved in lipopolysaccharide biosynthesis and decreased expression of polysaccharide capsule genes. (cdc.gov)

These results suggest that Esr41 plays important roles in the inverse regulation of LEE and flagellar gene expression. (nih.gov)
To also target low expressed essential genes, we included some variant steps that were expected to overcome the non-stringent regulation of the promoter carried by the expression vector used for the shotgun antisense libraries. (biomedcentral.com)
intriguing why these three genes are placed under BsaN/BicA regulation by the bacterium. (mirnaarray.com)
5 × 10-8) associated with chronic painful TMD, including loci near genes involved in the regulation of inflammatory and neuronal response. (iasp-pain.org)
By whole transcriptome analyses, we characterized glucosedependent and CcpA-dependent gene regulation in Clostridium difficile. (studyres.com)
About 18% of all C. difficile genes are regulated by glucose, for which 50% depend on CcpA for regulation. (studyres.com)
We further found via RNA-seq that GPF1 and CNF2 have similar mechanisms in gene expression regulation related to pathogenicity. (prelekara.sk)
These hormones regulate the body's growth, and are involved in cell to cell communication, control metabolic activity, sleep-wake homeostasis, and altered regulation or dysregulation of adaptive response in various physiologic and pathophysiologic states. (medscape.com)

The locus of enterocyte effacement-encoded regulator (Ler) is a regulatory protein that controls bacterial pathogenicity of enteropathogenic Escherichia coli (EPEC) and enterohemorrhagic Escherichia coli (EHEC). (wikipedia.org)
More specifically, Ler regulates the locus of enterocyte effacement (LEE) pathogenicity island genes, which are responsible for creating intestinal attachment and effacing lesions and subsequent diarrhea: LEE1, LEE2, and LEE3. (wikipedia.org)

Compared with reference strain Tohama I, all strains had increased expression and production of the type III secretion system apparatus. (cdc.gov)
Methods Bacterial strains and culture conditions Bacterial strains are listed in Table 3. (mirnaarray.com)
Here, we use comparative genomics approaches to understand how genetic variation amongst bacterial strains influences disease progression. (biomedcentral.com)
There are a number of challenges to applying GWAS to bacterial infections, including the difficulty of obtaining matched controls, multiple strain colonization and the possibility that causative strains may not be present when disease is detected. (biomedcentral.com)
By using RNA-seq data of prefrontal cortex (PFC) from 31 C57BL/6J X DBA/2J (BXD) recombinant inbred (RI) mouse strains and their parental strains under three chemical treatment groups saline control (CTL), diisopropylfluorophosphate (DFP), and corticosterone combined with diisopropylfluorophosphate (CORT+DFP), we identified Ccr6 as a candidate gene underlying individual differences in susceptibility to GWI. (cdc.gov)

Functional characterization of the identified regions found relationships between methylation at these loci and nearby genetic variation contributing to chronic painful TMD and with gene expression of proximal genes. (iasp-pain.org)
These findings reveal epigenetic contributions to chronic painful TMD through methylation of the genes FMOD, PM20D1, ZNF718, ZFP57, and RNF39, following the development of acute painful TMD. (iasp-pain.org)
The study also examined the genetic-epigenetic interactions with treatment response through differential methylation analysis, methylation quantitative trait loci, colocalization, and promoter-anchored chromatin interaction. (inra.fr)

Targeting of novel essential pathways is expected to play an important role in the discovery of new antibacterial agents against bacterial pathogens, such as Pseudomonas aeruginosa , that are difficult to control because of their ability to develop resistance, often multiple, to all current classes of clinical antibiotics. (biomedcentral.com)
Host Adaptation Predisposes Pseudomonas aeruginosa to Type VI Secretion System-Mediated Predation by the Burkholderia cepacia Complex. (wolfganglab.com)
The exopolysaccharide genes such as pilB, pslA, lecA, ureB and alginate genes are responsible for the formation of pseudomonas biofilms. (ukessays.com)

In Finland Yersinia is one of the main causes of bacterial diarrhea in addition of Salmonella and Campylobacter. (9pdf.co)

I also have a strong background in bacterial pathogenesis of both Animal and Plant Hosts. (ncl.ac.uk)
Although a number of bacteria and viruses have been show to influence serotonin signaling, no study has investigated the effect that serotonin has on bacterial pathogenesis. (grantome.com)
We analyzed the functions of these TF genes in development, pathogenesis, and stress responses under 9 conditions. (prelekara.sk)

When LEE expression is activated, flagellar gene expression is down-regulated because bacterial flagella induce the immune responses of host cells at the infection stage. (nih.gov)
We report here that a small regulatory RNA (sRNA), Esr41, mediates LEE repression and flagellar gene activation. (nih.gov)
In addition, Esr41 activated the expression of flagellar Class 3 genes by indirectly inducing the transcription of fliA, which encodes the sigma factor for flagellar synthesis. (nih.gov)
The flagellar filament is a major antigen recognized by hosts during bacterial infections. (ncl.ac.uk)
Our research focuses on the fundamental understanding of how bacteria regulate flagellar assembly, how a cell coordinates the assembly of unrelated molecular machines and how hosts recognise this important antigen. (ncl.ac.uk)
We complement our study on flagellar abundance with research to understanding how a filament is assembled from multiple flagellins, a trait maintained by many bacterial species. (ncl.ac.uk)
BsaN also suppresses other loci that are potentially counterproductive following intracellular localization, such as the fla1 flagellar motility and chemotaxis locus, which could lead to activation of cellular immunity pathways through PAMP recognition. (mirnaarray.com)

Based on their genomic organization, the transcription of these loci is likely driven from the promoter upstream of BPSS1512 tssM. (mirnaarray.com)

The identification of genes involved in replication, segregation, toxin-antitoxin systems and conjugation, would aid the design of drugs to prevent the survival or transmission of plasmids carrying pathogenic properties. (frontiersin.org)
Co-infection by different pathogenic bacterial species often increases the severity of the underlying condition, and are more difficult to treat [ 1 ]. (biomedcentral.com)

Multiple copies of esr41 abolished LEE expression by down-regulating the expression of ler and pch, which encode positive regulators of LEE. (nih.gov)
A significant number of bacterial species encode multiple copies of the filament building block - flagellin. (ncl.ac.uk)
The locus of the psl polysacchide genes encode the cells to adhere to a surface and thus maintaining the biofilm structure. (ukessays.com)

Translational gene-reporter fusion experiments revealed that Esr41 regulates ler expression at a post-transcriptional level, and pch transcription, probably via an unknown target of Esr41. (nih.gov)
We developed a high-throughput gene knockout system, and using this system, we obtained null mutants of 104 fungal-specific Zn2Cys6 transcription factor (TF) genes by screening 8741 primary transformants in M. oryzae. (prelekara.sk)
Systematic Analysis of ZnCys Transcription Factors Required for Development and Pathogenicity by High-Throughput Gene Knockout in the Rice Blast Fungus. (prelekara.sk)
Representative σ 66 -dependent gene transcription was repressed in the absence of rsbV1 or upon increased expression of RsbW, and increased upon elevated expression of RsbV1. (plos.org)

Plasmid stabilization/stability protein-encoding genes were observed in some plasmids but were not established for participating in plasmid segregation. (frontiersin.org)

This study also identified 43 growth-impairing inserts carrying multiple loci targeting 105 genes, of which 25 have homologs reported as essential in other bacteria. (biomedcentral.com)
The bacteria is gram negative, rod shaped and aerobic belongs to the bacterial family of pseudomonadanceae. (ukessays.com)
In both Gram-positive and Gram-negative bacteria, 'competence' for DNA uptake requires the coordinated action of rough-ly two dozen genes ( including some with regulatory func-tions ) organized within several operons. (asmblog.org)
For example, in vitro loss of TREM2 in microglia and macrophages leads to decreased phagocytosis of apoptotic neurons, cellular debris, and bacteria or bacterial products. (cisbio.jp)

We found that 61 Zn2Cys6 TF genes play indispensable and diversified roles in fungal development and pathogenicity. (prelekara.sk)

Following tissue damage, RNase T2 is secreted and participates in resistance against RNA viruses or functions as an alarm signaling molecule to regulate the host immune response and contributes to tissue remodeling and repair ( 12 , 13 ). (frontiersin.org)
The TREM family of receptors regulates the activity of various cell types in the immune system, including neutrophils, monocyte/macrophages, microglia, and dendritic cells. (cisbio.jp)
Microglia are strongly implicated in the genetics and neuropathology of late-onset AD and several AD-risk loci near immune genes have recently been identified. (cisbio.jp)
Herb-partitioned moxibustion may inhibit excessively activated autophagy and modulate the expression of immune-related factors by regulating the LKB1-mTOR-PI3KC signal transduction networks, thereby alleviating intestinal inflammation in CD rats. (hindawi.com)
Enrichment of these genes was seen in the immune, inflammation, cytokine, and neurological related categories. (cdc.gov)
The immune system consists of a large number of molecules and processes, and immunodeficiencies can therefore be caused by genetic alterations at many loci. (lu.se)

The see more secretion of HA-tagged BPSS1513 was not detected in in vitro secretion assays, although it is possible that the epitope tag could have interfered with secretion of BPSS1513, or that the assay was not performed at conditions buy SHP099 permissive for secretion. (mirnaarray.com)

Indeed, many of the same BsaN-regulated systems, namely T3SS and T6SS, are thought to act as "anti-predation determinants" that facilitate endosome escape and promote survival within bacteriovorus amoebae by manipulating eukaryotic pathways that are enough conserved from protists to humans [3]. (mirnaarray.com)

The intracellular distribution pattern suggests that RNase T2 may be involved in degrading exogenous or endogenous RNAs in lysosome and regulating mitochondrial RNA metabolism ( 6 - 8 ). (frontiersin.org)

Genome-wide meta-analysis increases to 71 the number of confirmed Crohn's disease susceptibility loci. (bioseek.eu)

During my career I have gained valuable experience working with a range of bacterial pathogens. (ncl.ac.uk)

In addition, metal transport was affected, and while iron-uptake genes such as the genes encoding the haemin uptake locus ( hmu ) were downregulated, expression of manganese transporter genes, such as feoB2 , was elevated in the presence of oxygen. (microbiologyresearch.org)

When differentiated human THP-1 cells were infected with MAP, the expression of various genes associated with stress responses and metabolism was altered in both host and MAP at 3 h post-infection. (biomedcentral.com)

The CcpA regulon comprises genes involved in sugar uptake, fermentation and amino acids metabolism, confirming the role of CcpA as a link between carbon and nitrogen pathways. (studyres.com)
The expression of genes encoding formate uptake transporter (PG0209) and formate tetrahydrofolate ligase ( fhs ) was drastically elevated, which indicates that formate metabolism plays a major role under aerobic conditions. (microbiologyresearch.org)
Notably, alterations in gene sets involved in arginine metabolism were observed in both the host and MAP, along with significant downregulation of NOS2 expression. (biomedcentral.com)

and 16 were "novel" essential genes with no homologs reported to have an essential role in other bacterial species. (biomedcentral.com)
Polymicrobial infections involve both detrimental and beneficial interactions between different bacterial species. (biomedcentral.com)
Antibiosis, which is defined by detrimental interactions between at least two species, usually involves the synthesis of antimicrobial molecules that inhibit growth or kill other bacterial species [ 18 ]. (biomedcentral.com)
The picture is si-milar in Vibrio - 'similar' refers to a great deal of protein homology among the numerous components in various species, despite vastly different regulatory circuits - for which the present model for competence involves 19+ genes, and, as core components: 1. (asmblog.org)

2020 " Bacterial dispersal and biogeography as underappreciated influences on phytobiomes. (baltruslab.com)

Fluorescence microscopy of chitin bead-co-lo-nizing cells of V. cholerae engineered to carry trans-lational fusions for the competen-ce protein ComEA-mCherry (red) and the T6SS sheath pro-tein VipA-sfGFP (green), both encod-ed at their native chromosomal locus. (asmblog.org)

Ler is regulated by many factors such as plasmid encoded regulator (Per), integration host factor, Fis, BipA, a positive regulatory loop involving GrlA, and quorum sensing mediated by luxS. (wikipedia.org)
Also, genes encoding gingipain protease secretion/maturation regulator ( porR ) and protease transporter ( porT ) had reduced expression in the presence of oxygen, which also correlated with reduced protease activities under aerobic conditions. (microbiologyresearch.org)

We aimed to identify novel essential genes in P. aeruginosa by shotgun antisense screening. (biomedcentral.com)
For the first time in P. aeruginosa , we applied regulated antisense RNA expression and showed the feasibility of this technology for the identification of novel essential genes. (biomedcentral.com)
Endogenous antisense RNAs function as regulators of gene expression by a variety of mechanisms. (genomicglossaries.com)
Synthetic antisense RNAs are used to effect the functioning of specific genes for investigative or therapeutic purposes. (genomicglossaries.com)
presents an opportunity to manipulate gene expression within the cells to treat various diseases, and acts as a powerful tool for studying gene function utilizing antisense agents to manage the diseases by regulating the expression of the specific factor that actually causes the particular disease. (genomicglossaries.com)
Highly specific and effective gene silencing of any disease can be achieved by an accurate knowledge of the target mRNA sequence and rational design of its complementary antisense agents for the downregulation of its protein message. (genomicglossaries.com)

Urinary tract infections (UTIs) are a common bacterial infection with symptoms that include urinary frequency, urgency to void, dysuria and abdominal pain. (ncl.ac.uk)
compared to some bacterial immunities, it is of relatively long duration, but this may be the result of infection-i. rather than being peculiar to virus infection per se, since it occurs also in bacterial i. after infections such as typhoid fever. (theodora.com)
These subtypes are clinically indistinguishable from one another, except for the fact that patients with GSD type Ib have altered neutrophil functions predisposing them to gram-positive bacterial infections. (medscape.com)

The genes responsible for the biofilm formation are quantified by comparing the planktonic and biofilm samples. (ukessays.com)

2001. Discrimination of bacterial lipoproteins by Toll-like receptor 6. (invivogen.com)

In addition, expression of M2 in primary murine B cells leads to secretion of high levels of IL-10 along with enhanced proliferation and survival. (prelekara.sk)

most other cases are caused by mutations in the PKD2 gene on chromosome 4, which codes for polycystin 2. (msdmanuals.com)
polycystin 2 may function as an ion channel, with mutations causing fluid secretion into cysts. (msdmanuals.com)
For practical purposes, depending on the enzyme activity and the presence of mutations in the G6Pase and T genes, respectively, GSD type I may be subdivided into 2 major forms. (medscape.com)
It is an autosomal recessive disorder in which there is an AGL gene mutations which causes deficiency in glycogen debranchinging enzyme and limited storage of dextrin. (medscape.com)

My expertise utilise bacterial genetics to study fundamental aspects of the bacterial life cycle and host-microbe interactions. (ncl.ac.uk)
My laboratory uses, bacterial genetics, protein biochemistry, structural biology, bioinformatics and microscopic techniques to validate our genetic findings. (ncl.ac.uk)

On the host side, upregulation of inflammatory cytokine genes was observed along with pattern recognition receptor genes. (biomedcentral.com)

Recently, we have shown that, besides their effect on genome structure and stability, ISs can affect expression profiles of neighboring genes by IS element-specific promoters ( 21 ). (cdc.gov)

AIM: We evaluated whether biomarkers in vaginal secretions and/or differences in the dominant bacterium in the vaginal microbiome predicted the risk for spontaneous preterm birth in primigravid women with a cervical length >25mm. (bvsalud.org)

Consistent with the latter observation, we show that IRF4 can regulate the IL-10 promoter in B cells. (prelekara.sk)

LEE1, 2, and 3 carry the information necessary for a type III secretion system. (wikipedia.org)

A biological process that is crucial for gene expression in most animal life, including humans. (genomicglossaries.com)

Because alternative RNA splicing allows different types of mRNA molecules to be created from a single gene, it generates the diversity of protein function and structure that is essential to complex organisms. (genomicglossaries.com)

Many functional GI disorders are associated with alterations in serotonin signaling, but the effect of serotonin signaling on bacterial-mediated GI disorders remains unknown. (grantome.com)
The impact is enhanced if coupled with the presence of functional genes for antibiotic resistance. (frontiersin.org)
2007) Genome-wide functional analysis of pathogenicity genes in the rice blast fungus. (prelekara.sk)
Gene expression and functional deficits underlie TREM2-knockout microglia responses in human models of AD. (cisbio.jp)

Replication genes were not identified in some plasmids, a situation that has led to the possibility of host interaction involvement. (frontiersin.org)

Microarray analysis revealed alteration in gene expression in the presence of 6 % oxygen. (microbiologyresearch.org)

Vasopressin promotes cell growth and fluid secretion via the cyclic AMP pathway, which leads to increase in the size and number of cysts in polycystic kidney disease. (msdmanuals.com)
It produces 2 hormones: vasopressin, which causes blood pressure to rise and regulates the amount of water in the body's cells, and oxytocin, which causes the uterus to contract during childbirth and lactation to begin. (medscape.com)

Rotation of the filament is achieved via the action of a universal joint known as the hook and a basal motor anchored into the bacterial cell envelope. (ncl.ac.uk)
MAP upregulates stress-responsive global gene regulators, such as two-component systems and sigma factors, in response to oxidative and cell wall stress. (biomedcentral.com)

We detected the potential link between observed effects and insertion sequence element-induced changes in gene context only for a few genes. (cdc.gov)

bored to see yet another post on horizontal gene transfer (HGT). (asmblog.org)
pisi PP1 highlights the importance of multiple modes of horizontal gene transfer during phytopathogen evolution. (baltruslab.com)

The expression and secretion of RNase T2 can be induced in response to a variety of tissue injury stimuli or oxidative stress ( 10 , 11 ). (frontiersin.org)

Anatomy52

Organisms18

Diseases5

Chemicals and Drugs83

Analytical, Diagnostic and Therapeutic Techniques and Equipment29

Phenomena and Processes75

Disciplines and Occupations2

Technology, Industry, Agriculture1

Information Science4

Health Care1

Virulence13

Encodes a type three2

Pathogen4

Proteins7

Biosynthesis1

Regulation8

Enterocyte effacement2

Strains5

Methylation3

Pseudomonas3

Salmonella1

Pathogenesis3

Flagellar7

Genomic1

Pathogenic2

Encode3

Transcription4

Protein-encod1

Bacteria4

Roles1

Immune6

Assays1

T3SS1

Intracellular1

Susceptibility1

Pathogens1

Iron-uptake1

Various genes1

Metabolism3

Species4

20201

T6SS1

Regulator2

Antisense6

Infections3

Biofilm formation1

20011

Murine1

Mutations4

Genetics2

Cytokine1

Promoters1

Bacterium1

Promoter1

Type1

Humans1

Organisms1

Functional4

Replication1

Analysis revealed1

Vasopressin2

Cell2

Changes in gene1

Horizontal gene tra2

Oxidative1